Starting /dee2/code/volunteer_pipeline.sh SRR6941608 current disk space = 1551148720128 free memory = 1603754868 SRR6941608 SRAfilesize a78912aef42dc0bc38c105dc97f0ca53 SRR6941608.sra SRR6941608.sra file validated SRR6941608 is paired end SRR6941608 is conventional basespace SRR6941608 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941608_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.66075 25.0 18.0 33.0 18.0 33.0 2 25.55075 27.0 18.0 31.0 18.0 33.0 3 29.2175 30.0 27.0 33.0 25.0 33.0 4 31.64575 33.0 31.0 33.0 29.0 33.0 5 32.4855 33.0 33.0 33.0 32.0 33.0 6 36.31775 38.0 36.0 38.0 34.0 38.0 7 36.655 38.0 37.0 38.0 34.0 38.0 8 37.26125 38.0 38.0 38.0 36.0 38.0 9 37.506 38.0 38.0 38.0 37.0 38.0 10-14 37.4475 38.0 38.0 38.0 37.2 38.0 15-19 37.31425 38.0 38.0 38.0 36.8 38.0 20-24 37.4757 38.0 38.0 38.0 37.4 38.0 25-29 37.53085 38.0 38.0 38.0 37.8 38.0 30-34 37.56975 38.0 38.0 38.0 38.0 38.0 35-39 37.515299999999996 38.0 38.0 38.0 38.0 38.0 40-44 37.4457 38.0 38.0 38.0 37.6 38.0 45-49 37.45075 38.0 38.0 38.0 37.4 38.0 50-54 37.3072 38.0 38.0 38.0 36.8 38.0 55-59 37.1575 38.0 38.0 38.0 36.4 38.0 60-64 37.26559999999999 38.0 38.0 38.0 36.6 38.0 65-69 37.2496 38.0 38.0 38.0 36.8 38.0 70-74 37.26545 38.0 38.0 38.0 36.8 38.0 75-79 37.212900000000005 38.0 38.0 38.0 36.0 38.0 80-84 37.233799999999995 38.0 38.0 38.0 36.4 38.0 85-89 37.12105 38.0 38.0 38.0 36.0 38.0 90-94 36.01195 38.0 36.8 38.0 30.0 38.0 95-99 36.653099999999995 38.0 37.8 38.0 34.4 38.0 100-104 37.04555 38.0 38.0 38.0 35.8 38.0 105-109 36.93545 38.0 38.0 38.0 35.4 38.0 110-114 36.91595 38.0 38.0 38.0 35.0 38.0 115-119 36.72215 38.0 38.0 38.0 34.8 38.0 120-124 36.54865 38.0 38.0 38.0 34.2 38.0 125-129 36.4773 38.0 38.0 38.0 34.0 38.0 130-134 36.52165000000001 38.0 38.0 38.0 34.2 38.0 135-139 36.201 38.0 38.0 38.0 33.6 38.0 140-144 36.019 38.0 37.6 38.0 33.0 38.0 145-149 35.45175 38.0 36.2 38.0 32.2 38.0 150-151 32.69225 37.0 33.5 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 0.0 16 1.0 17 0.0 18 1.0 19 0.0 20 3.0 21 2.0 22 3.0 23 5.0 24 3.0 25 5.0 26 10.0 27 10.0 28 21.0 29 27.0 30 26.0 31 47.0 32 56.0 33 85.0 34 140.0 35 208.0 36 597.0 37 2748.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.84473897754936 13.253989721395726 7.330267784690289 37.57100351636462 2 23.911955977988995 14.7823911955978 30.190095047523762 31.115557778889446 3 20.225 21.575 28.825 29.375 4 23.1 31.724999999999998 23.175 22.0 5 23.325000000000003 35.199999999999996 23.724999999999998 17.75 6 18.375 38.475 22.650000000000002 20.5 7 14.224999999999998 26.5 42.925000000000004 16.35 8 18.625 26.724999999999998 28.95 25.7 9 16.650000000000002 23.9 32.975 26.474999999999998 10-14 19.900000000000002 31.790000000000003 24.215 24.095 15-19 21.505 28.310000000000002 25.650000000000002 24.535 20-24 20.195 29.715000000000003 26.119999999999997 23.97 25-29 22.305 29.53 25.915 22.25 30-34 22.040000000000003 30.214999999999996 24.785 22.96 35-39 21.235 29.630000000000003 25.865 23.27 40-44 20.01 28.925 26.805 24.26 45-49 20.325 29.625 27.22 22.830000000000002 50-54 20.979999999999997 28.67 26.724999999999998 23.625 55-59 20.375 29.94 25.480000000000004 24.205 60-64 20.29 29.32 26.634999999999998 23.755000000000003 65-69 21.055 29.060000000000002 25.19 24.695 70-74 21.759999999999998 29.145 24.785 24.310000000000002 75-79 20.615 29.505 25.669999999999998 24.21 80-84 22.185 28.96 24.77 24.085 85-89 21.495 28.384999999999998 26.435 23.685000000000002 90-94 20.95 29.659999999999997 25.405 23.985 95-99 20.9 29.49 24.855 24.755 100-104 20.775 30.365 24.45 24.41 105-109 21.759999999999998 28.475 25.39 24.375 110-114 20.8 28.999999999999996 25.88 24.32 115-119 20.785 29.909999999999997 24.505 24.8 120-124 20.395 30.19 23.5 25.915 125-129 21.345 30.064999999999998 23.98 24.610000000000003 130-134 22.134999999999998 30.795 22.535 24.535 135-139 22.345000000000002 29.79 24.785 23.080000000000002 140-144 22.59 29.494999999999997 24.44 23.474999999999998 145-149 21.490000000000002 28.78 24.245 25.485000000000003 150-151 20.4625 30.6375 23.525 25.374999999999996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 2.0 21 3.5 22 3.0 23 2.0 24 2.0 25 6.5 26 9.5 27 10.0 28 14.0 29 20.0 30 23.5 31 25.0 32 26.5 33 35.5 34 41.5 35 47.0 36 104.5 37 212.5 38 222.0 39 186.0 40 225.5 41 254.5 42 225.5 43 208.0 44 209.0 45 203.0 46 169.0 47 121.5 48 121.0 49 102.0 50 82.0 51 86.0 52 74.5 53 69.5 54 94.5 55 126.0 56 125.5 57 98.5 58 75.5 59 70.0 60 60.0 61 35.5 62 25.0 63 21.5 64 21.0 65 22.0 66 16.0 67 10.5 68 7.5 69 5.5 70 4.0 71 6.5 72 8.0 73 5.0 74 4.0 75 4.0 76 2.0 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 7.575 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.75 #Duplication Level Percentage of deduplicated Percentage of total 1 85.85365853658537 61.6 2 7.700348432055749 11.05 3 2.0905923344947737 4.5 4 1.4285714285714286 4.1000000000000005 5 0.8362369337979094 3.0 6 0.5226480836236934 2.25 7 0.313588850174216 1.575 8 0.3832752613240418 2.1999999999999997 9 0.13937282229965156 0.8999999999999999 >10 0.7317073170731708 8.825 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 49 1.225 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 28 0.7000000000000001 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 24 0.6 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 23 0.575 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 21 0.525 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 21 0.525 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 18 0.44999999999999996 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 16 0.4 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 16 0.4 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 13 0.325 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 13 0.325 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 13 0.325 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 12 0.3 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 12 0.3 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 11 0.27499999999999997 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 11 0.27499999999999997 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 11 0.27499999999999997 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 11 0.27499999999999997 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 10 0.25 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 10 0.25 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 10 0.25 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 9 0.22499999999999998 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 9 0.22499999999999998 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 9 0.22499999999999998 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 8 0.2 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 8 0.2 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 8 0.2 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 8 0.2 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 8 0.2 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 8 0.2 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 8 0.2 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 8 0.2 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 7 0.17500000000000002 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 7 0.17500000000000002 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 7 0.17500000000000002 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 7 0.17500000000000002 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 7 0.17500000000000002 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 7 0.17500000000000002 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 7 0.17500000000000002 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 7 0.17500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 7 0.17500000000000002 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 6 0.15 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 6 0.15 No Hit CAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGG 6 0.15 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 6 0.15 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 6 0.15 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 6 0.15 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 6 0.15 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 6 0.15 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGAT 5 0.125 No Hit AAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAAC 5 0.125 No Hit AGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit ATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCG 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit GGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGC 5 0.125 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 5 0.125 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 5 0.125 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 5 0.125 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 5 0.125 No Hit ATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 5 0.125 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 5 0.125 No Hit NTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit NCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit GCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTCTGTTCAGGGTT 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.25 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.6375 0.0 0.0 0.0 0.0 86-87 0.7875 0.0 0.0 0.0 0.0 88-89 0.9874999999999999 0.0 0.0 0.0 0.0 90-91 1.2625000000000002 0.0 0.0 0.0 0.0 92-93 1.55 0.0 0.0 0.0 0.0 94-95 1.8250000000000002 0.0 0.0 0.0 0.0 96-97 2.0875 0.0 0.0 0.0 0.0 98-99 2.2750000000000004 0.0 0.0 0.0 0.0 100-101 2.6375 0.0 0.0 0.0 0.0 102-103 3.0 0.0 0.0 0.0 0.0 104-105 3.35 0.0 0.0 0.0 0.0 106-107 3.725 0.0 0.0 0.0 0.0 108-109 4.45 0.0 0.0 0.0 0.0 110-111 4.925 0.0 0.0 0.0 0.0 112-113 5.6125 0.0 0.0 0.0 0.0 114-115 6.3375 0.0 0.0 0.0 0.0 116-117 6.925 0.0 0.0 0.0 0.0 118-119 7.5125 0.0 0.0 0.0 0.0 120-121 8.2 0.0 0.0 0.0 0.0 122-123 8.9875 0.0 0.0 0.0 0.0 124-125 9.725000000000001 0.0 0.0 0.0 0.0 126-127 10.4875 0.0 0.0 0.0 0.0 128-129 11.1875 0.0 0.0 0.0 0.0 130-131 11.95 0.0 0.0 0.0 0.0 132-133 12.712499999999999 0.0 0.0 0.0 0.0 134-135 13.412500000000001 0.0 0.0 0.0 0.0 136-137 14.3 0.0 0.0 0.0 0.0 138-139 14.9125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR6941608 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941608_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.03 33.0 33.0 34.0 32.0 34.0 2 33.0735 34.0 33.0 34.0 33.0 34.0 3 33.104 34.0 33.0 34.0 33.0 34.0 4 33.11725 34.0 33.0 34.0 33.0 34.0 5 33.11375 34.0 33.0 34.0 33.0 34.0 6 37.324 38.0 38.0 38.0 37.0 38.0 7 37.26075 38.0 38.0 38.0 37.0 38.0 8 37.3145 38.0 38.0 38.0 37.0 38.0 9 37.2665 38.0 38.0 38.0 37.0 38.0 10-14 36.9452 38.0 38.0 38.0 36.0 38.0 15-19 37.13555 38.0 38.0 38.0 36.8 38.0 20-24 37.2348 38.0 38.0 38.0 37.0 38.0 25-29 37.1841 38.0 38.0 38.0 37.0 38.0 30-34 36.799699999999994 38.0 38.0 38.0 36.2 38.0 35-39 36.67685 38.0 38.0 38.0 35.2 38.0 40-44 37.0486 38.0 38.0 38.0 36.8 38.0 45-49 37.10485 38.0 38.0 38.0 37.0 38.0 50-54 37.1128 38.0 38.0 38.0 36.8 38.0 55-59 36.9739 38.0 38.0 38.0 36.4 38.0 60-64 37.045550000000006 38.0 38.0 38.0 36.8 38.0 65-69 36.92100000000001 38.0 38.0 38.0 36.4 38.0 70-74 36.7536 38.0 38.0 38.0 35.4 38.0 75-79 36.876549999999995 38.0 38.0 38.0 36.0 38.0 80-84 36.6302 38.0 38.0 38.0 35.2 38.0 85-89 36.35615 38.0 37.8 38.0 34.0 38.0 90-94 36.746750000000006 38.0 38.0 38.0 35.4 38.0 95-99 36.5983 38.0 38.0 38.0 35.0 38.0 100-104 35.7718 38.0 37.2 38.0 30.4 38.0 105-109 35.93300000000001 38.0 37.2 38.0 32.4 38.0 110-114 36.33370000000001 38.0 38.0 38.0 34.0 38.0 115-119 36.26485 38.0 38.0 38.0 34.0 38.0 120-124 35.89975 38.0 37.6 38.0 32.8 38.0 125-129 35.62865000000001 38.0 37.0 38.0 32.2 38.0 130-134 35.489 38.0 36.4 38.0 31.8 38.0 135-139 31.583550000000002 36.6 25.2 38.0 20.2 38.0 140-144 33.1721 37.0 31.2 38.0 22.6 38.0 145-149 34.22345 38.0 35.2 38.0 27.2 38.0 150-151 29.593625000000003 35.5 28.0 38.0 11.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 6.0 4 3.0 5 1.0 6 1.0 7 1.0 8 1.0 9 2.0 10 1.0 11 1.0 12 3.0 13 0.0 14 3.0 15 2.0 16 5.0 17 2.0 18 3.0 19 4.0 20 2.0 21 6.0 22 8.0 23 4.0 24 5.0 25 12.0 26 18.0 27 34.0 28 23.0 29 23.0 30 35.0 31 48.0 32 90.0 33 93.0 34 132.0 35 284.0 36 760.0 37 2376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.575 21.0 15.024999999999999 22.400000000000002 2 28.214107053526767 19.984992496248125 33.59179589794897 18.209104552276138 3 20.445445445445447 21.82182182182182 38.83883883883884 18.893893893893893 4 24.568426319739807 31.923942957217914 25.794345759319487 17.713284963722792 5 26.99524643482612 32.49937453089817 23.067300475356518 17.43807855891919 6 21.625 34.075 25.074999999999996 19.225 7 18.475 19.675 42.9 18.95 8 21.25 23.0 29.875 25.874999999999996 9 21.525 22.125 33.0 23.35 10-14 25.230000000000004 25.505 28.73 20.535 15-19 24.15 25.14 30.025000000000002 20.685000000000002 20-24 25.11 24.565 29.830000000000002 20.495 25-29 24.41744174417442 25.012501250125013 29.572957295729573 20.997099709971 30-34 24.64492898579716 25.11002200440088 30.136027205441092 20.10902180436087 35-39 25.10625531276564 25.321266063303167 28.921446072303613 20.651032551627583 40-44 24.795 25.569999999999997 28.96 20.674999999999997 45-49 24.234846969393878 26.295259051810362 28.605721144228845 20.86417283456691 50-54 24.292429242924293 25.327532753275328 28.972897289728973 21.407140714071407 55-59 23.330000000000002 26.040000000000003 29.244999999999997 21.385 60-64 24.087408740874086 25.502550255025504 29.312931293129314 21.097109710971097 65-69 24.08222466740022 25.677703310993298 28.683605081524455 21.556466940082025 70-74 24.801200300075017 25.916479119779943 28.84721180295074 20.4351087771943 75-79 25.02125106255313 24.8162408120406 29.35146757337867 20.811040552027603 80-84 24.66746674667467 25.28752875287529 29.63796379637964 20.407040704070408 85-89 24.82 26.16 28.299999999999997 20.72 90-94 24.52 25.424999999999997 28.599999999999998 21.455 95-99 24.37 25.45 29.28 20.9 100-104 25.285000000000004 25.845000000000002 28.64 20.23 105-109 25.585 24.975 29.03 20.41 110-114 25.317531753175317 25.452545254525454 29.17291729172917 20.05700570057006 115-119 25.687568756875688 26.117611761176118 28.007800780078007 20.187018701870187 120-124 25.97759775977598 26.267626762676265 27.102710271027103 20.652065206520653 125-129 26.366318315915795 26.46132306615331 27.181359067953398 19.9909995499775 130-134 26.325 26.57 27.560000000000002 19.545 135-139 26.015 26.150000000000002 27.92 19.915 140-144 27.591379568978446 25.726286314315715 28.026401320066004 18.65593279663983 145-149 27.200000000000003 25.840000000000003 27.965 18.995 150-151 26.625 26.2875 28.825 18.2625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 0.5 20 2.0 21 2.0 22 3.0 23 7.0 24 7.5 25 5.5 26 9.0 27 14.5 28 16.0 29 20.0 30 29.5 31 36.5 32 38.0 33 43.5 34 56.0 35 70.5 36 100.0 37 129.0 38 156.5 39 195.0 40 219.5 41 218.5 42 211.0 43 217.0 44 227.5 45 195.5 46 164.5 47 151.0 48 108.0 49 92.5 50 94.0 51 97.5 52 88.5 53 75.0 54 104.0 55 126.0 56 114.5 57 92.0 58 74.5 59 67.5 60 64.5 61 56.5 62 43.0 63 31.0 64 24.0 65 18.0 66 14.5 67 12.0 68 7.5 69 7.5 70 8.0 71 5.5 72 4.0 73 4.5 74 4.0 75 2.5 76 3.5 77 3.5 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.1 4 0.075 5 0.075 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.02 35-39 0.005 40-44 0.0 45-49 0.02 50-54 0.01 55-59 0.0 60-64 0.01 65-69 0.03 70-74 0.025 75-79 0.005 80-84 0.01 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.01 115-119 0.01 120-124 0.01 125-129 0.005 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.95 #Duplication Level Percentage of deduplicated Percentage of total 1 82.62861169837915 58.62500000000001 2 9.372797744890768 13.3 3 3.5236081747709656 7.5 4 1.656095842142354 4.7 5 0.8456659619450317 3.0 6 0.4580690627202256 1.95 7 0.42283298097251587 2.1 8 0.1761804087385483 1.0 9 0.14094432699083861 0.8999999999999999 >10 0.7751937984496124 6.925000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 23 0.575 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 18 0.44999999999999996 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 16 0.4 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 16 0.4 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 15 0.375 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 15 0.375 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 13 0.325 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 12 0.3 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 11 0.27499999999999997 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 11 0.27499999999999997 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 11 0.27499999999999997 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 11 0.27499999999999997 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 11 0.27499999999999997 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 11 0.27499999999999997 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 11 0.27499999999999997 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 11 0.27499999999999997 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 11 0.27499999999999997 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 10 0.25 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 10 0.25 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 10 0.25 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 10 0.25 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 10 0.25 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 9 0.22499999999999998 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 9 0.22499999999999998 No Hit ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC 9 0.22499999999999998 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 8 0.2 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 8 0.2 No Hit TGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACT 8 0.2 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 7 0.17500000000000002 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 7 0.17500000000000002 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 7 0.17500000000000002 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 7 0.17500000000000002 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 7 0.17500000000000002 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 6 0.15 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 6 0.15 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 6 0.15 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 6 0.15 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 6 0.15 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit TCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTG 6 0.15 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 6 0.15 No Hit TCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAAC 5 0.125 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 5 0.125 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit GCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTAT 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 5 0.125 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 5 0.125 No Hit TATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGG 5 0.125 No Hit TGACAATCTCACAAGGATGAAATACCAGTAATTTTTTATTTACTGGTTGA 5 0.125 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit GAAGAAATGATGAAGAGAGCTGTTTTTGCGAGAGAATTAGGTGTTCCTAT 5 0.125 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.25 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.6375 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 1.0625 0.0 0.0 0.0 0.0 90-91 1.3624999999999998 0.0 0.0 0.0 0.0 92-93 1.65 0.0 0.0 0.0 0.0 94-95 1.8875000000000002 0.0 0.0 0.0 0.0 96-97 2.1125 0.0 0.0 0.0 0.0 98-99 2.3 0.0 0.0 0.0 0.0 100-101 2.6125 0.0 0.0 0.0 0.0 102-103 2.9749999999999996 0.0 0.0 0.0 0.0 104-105 3.3375000000000004 0.0 0.0 0.0 0.0 106-107 3.725 0.0 0.0 0.0 0.0 108-109 4.4375 0.0 0.0 0.0 0.0 110-111 4.95 0.0 0.0 0.0 0.0 112-113 5.6375 0.0 0.0 0.0 0.0 114-115 6.3375 0.0 0.0 0.0 0.0 116-117 6.925 0.0 0.0 0.0 0.0 118-119 7.5375 0.0 0.0 0.0 0.0 120-121 8.2 0.0 0.0 0.0 0.0 122-123 8.9625 0.0 0.0 0.0 0.0 124-125 9.65 0.0 0.0 0.0 0.0 126-127 10.3125 0.0 0.0 0.0 0.0 128-129 10.875 0.0 0.0 0.0 0.0 130-131 11.4875 0.0 0.0 0.0 0.0 132-133 12.1125 0.0 0.0 0.0 0.0 134-135 12.600000000000001 0.0 0.0 0.0 0.0 136-137 13.225000000000001 0.0 0.0 0.0 0.0 138-139 13.850000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGCTT 10 0.006830828 145.0 8 CCTTACC 10 0.006830828 145.0 1 TACCAGG 10 0.006830828 145.0 4 GGGCTTG 10 0.006830828 145.0 9 TGTAGGA 40 0.0076550315 18.125 135-139 >>END_MODULE Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161558 spots for SRR6941608.sra Written 1161558 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra Read 1161547 spots for SRR6941608.sra Written 1161547 spots for SRR6941608.sra SRR ids: ['SRR6941608.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9jecz15k SRR6941608.sra spots: 23230951 blocks: [[1, 1161547], [1161548, 2323094], [2323095, 3484641], [3484642, 4646188], [4646189, 5807735], [5807736, 6969282], [6969283, 8130829], [8130830, 9292376], [9292377, 10453923], [10453924, 11615470], [11615471, 12777017], [12777018, 13938564], [13938565, 15100111], [15100112, 16261658], [16261659, 17423205], [17423206, 18584752], [18584753, 19746299], [19746300, 20907846], [20907847, 22069393], [22069394, 23230951]] SRR6941608 file size 7850506 SRR6941608 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941608 SRR6941608_1.fastq SRR6941608_2.fastq Input file: SRR6941608_1.fastq Paired file: SRR6941608_2.fastq trimmed: SRR6941608-trimmed-pair1.fastq, SRR6941608-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 13:04:23 2024 >> started Fri Dec 6 13:04:52 2024 >> done (29.153s) 23230951 read pairs processed; of these: 39765 ( 0.17%) short read pairs filtered out after trimming by size control 34973 ( 0.15%) empty read pairs filtered out after trimming by size control 23156213 (99.68%) read pairs available; of these: 10176049 (43.95%) trimmed read pairs available after processing 12980164 (56.05%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 4 0.00% 21 2 0.00% 22 2 0.00% 23 3 0.00% 24 4 0.00% 25 3 0.00% 26 3 0.00% 27 26 0.00% 28 5 0.00% 29 8 0.00% 30 5 0.00% 31 7 0.00% 32 11 0.00% 33 6 0.00% 34 12 0.00% 35 14 0.00% 36 5 0.00% 37 10 0.00% 38 10 0.00% 39 31 0.00% 40 31 0.00% 41 43 0.00% 42 55 0.00% 43 47 0.00% 44 51 0.00% 45 61 0.00% 46 62 0.00% 47 86 0.00% 48 95 0.00% 49 127 0.00% 50 183 0.00% 51 195 0.00% 52 221 0.00% 53 271 0.00% 54 256 0.00% 55 385 0.00% 56 423 0.00% 57 426 0.00% 58 558 0.00% 59 660 0.00% 60 724 0.00% 61 918 0.00% 62 1184 0.01% 63 1333 0.01% 64 1444 0.01% 65 1598 0.01% 66 1824 0.01% 67 1843 0.01% 68 2375 0.01% 69 2580 0.01% 70 3099 0.01% 71 3508 0.02% 72 4462 0.02% 73 4851 0.02% 74 5093 0.02% 75 5955 0.03% 76 6455 0.03% 77 7273 0.03% 78 7945 0.03% 79 9331 0.04% 80 10289 0.04% 81 11952 0.05% 82 13228 0.06% 83 14581 0.06% 84 17291 0.07% 85 20778 0.09% 86 21146 0.09% 87 22589 0.10% 88 25885 0.11% 89 26142 0.11% 90 29526 0.13% 91 29030 0.13% 92 34145 0.15% 93 35213 0.15% 94 37609 0.16% 95 42298 0.18% 96 40362 0.17% 97 41760 0.18% 98 43963 0.19% 99 45934 0.20% 100 47221 0.20% 101 49938 0.22% 102 54264 0.23% 103 52945 0.23% 104 58823 0.25% 105 61184 0.26% 106 60451 0.26% 107 62932 0.27% 108 67046 0.29% 109 66873 0.29% 110 68780 0.30% 111 70932 0.31% 112 73957 0.32% 113 72319 0.31% 114 82815 0.36% 115 84031 0.36% 116 86222 0.37% 117 86203 0.37% 118 83199 0.36% 119 83690 0.36% 120 84518 0.36% 121 88946 0.38% 122 103049 0.45% 123 100091 0.43% 124 101237 0.44% 125 105231 0.45% 126 100849 0.44% 127 102344 0.44% 128 100238 0.43% 129 107292 0.46% 130 98776 0.43% 131 106539 0.46% 132 115380 0.50% 133 104105 0.45% 134 114438 0.49% 135 108859 0.47% 136 113212 0.49% 137 110946 0.48% 138 118874 0.51% 139 121758 0.53% 140 121456 0.52% 141 139245 0.60% 142 133226 0.58% 143 142841 0.62% 144 152926 0.66% 145 180085 0.78% 146 195761 0.85% 147 228656 0.99% 148 321335 1.39% 149 583498 2.52% 150 3854590 16.65% 151 12980164 56.05% 23156213 reads passed initial QC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.40 fanout-score-rank=26 prefix-density=0.41 prefix-fanout=2.4 sequence=GTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGTTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=39 fanout-score=54.90 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=5.5 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=2.92 fanout-score-rank=20 prefix-density=0.83 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGGA criterion=fanout-score sequence-density=0.02 sequence-density-rank=30 fanout-score=21.58 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=6.5 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR6941608 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 13:05:27 Started mapping on | Dec 06 13:05:27 Finished on | Dec 06 13:07:30 Mapping speed, Million of reads per hour | 677.74 Number of input reads | 23156213 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 13184621 Uniquely mapped reads % | 56.94% Average mapped length | 292.15 Number of splices: Total | 2534467 Number of splices: Annotated (sjdb) | 2289117 Number of splices: GT/AG | 2419369 Number of splices: GC/AG | 29376 Number of splices: AT/AC | 9244 Number of splices: Non-canonical | 76478 Mismatch rate per base, % | 0.18% Deletion rate per base | 0.01% Deletion average length | 1.55 Insertion rate per base | 0.01% Insertion average length | 1.76 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8447986 % of reads mapped to multiple loci | 36.48% Number of reads mapped to too many loci | 111698 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.77% % of reads unmapped: other | 2.33% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1537874 1537874 1537874 N_multimapping 8447986 8447986 8447986 N_noFeature 5856230 12747212 6031216 N_ambiguous 496528 8309 240065 UnstrandedReadsAssigned:6831863 PositiveStrandReadsAssigned:429100 NegativeStrandReadsAssigned:6913340 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR6941608 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941608-trimmed-pair1.fastq SRR6941608-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,156,213 reads, 12,056,578 reads pseudoaligned [quant] estimated average fragment length: 213.592 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,113 rounds 52973 SRR6941608.ke.tsv 35125 SRR6941608.se.tsv 88098 total ==> SRR6941608.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 724.064 0 0 PNS24247 1044 831.408 7.21938 0.663161 PNS24249 1928 1715.41 10.0827 0.448893 PNS24246 1044 831.408 7.21938 0.663161 PNS24248 1044 831.408 7.21938 0.663161 PNS24244 1471 1258.41 11.2592 0.68331 PNS24243 293 118.794 0 0 KQK14069 1603 1390.41 736.212 40.4384 KQK14071 474 272.542 27.2747 7.64293 ==> SRR6941608.se.tsv <== BRADI_1g14170v3 900 BRADI_1g53295v3 18 BRADI_1g59795v3 18 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 49 BRADI_1g74790v3 22 BRADI_1g09890v3 0 BRADI_1g77505v3 32 BRADI_1g48960v3 0 SRR6941608 completed mapping pipeline successfully