Starting /dee2/code/volunteer_pipeline.sh SRR6941609 current disk space = 1551161946112 free memory = 1602323528 SRR6941609 SRAfilesize 03ca8e8dc71c07796205a153046ff83c SRR6941609.sra SRR6941609.sra file validated SRR6941609 is paired end SRR6941609 is conventional basespace SRR6941609 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941609_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4835 34.0 33.0 34.0 32.0 34.0 2 33.09625 34.0 33.0 34.0 32.0 34.0 3 33.1845 34.0 33.0 34.0 32.0 34.0 4 33.359 34.0 33.0 34.0 33.0 34.0 5 33.45725 34.0 33.0 34.0 33.0 34.0 6 37.28325 38.0 38.0 38.0 36.0 38.0 7 37.587 38.0 38.0 38.0 37.0 38.0 8 37.6175 38.0 38.0 38.0 38.0 38.0 9 37.617 38.0 38.0 38.0 38.0 38.0 10-14 37.657349999999994 38.0 38.0 38.0 38.0 38.0 15-19 37.691649999999996 38.0 38.0 38.0 38.0 38.0 20-24 37.665200000000006 38.0 38.0 38.0 38.0 38.0 25-29 37.64065 38.0 38.0 38.0 38.0 38.0 30-34 37.501400000000004 38.0 38.0 38.0 38.0 38.0 35-39 37.54905 38.0 38.0 38.0 38.0 38.0 40-44 37.57215 38.0 38.0 38.0 38.0 38.0 45-49 37.595150000000004 38.0 38.0 38.0 38.0 38.0 50-54 37.53939999999999 38.0 38.0 38.0 38.0 38.0 55-59 37.5128 38.0 38.0 38.0 38.0 38.0 60-64 37.50175 38.0 38.0 38.0 37.8 38.0 65-69 37.3688 38.0 38.0 38.0 37.2 38.0 70-74 37.331849999999996 38.0 38.0 38.0 37.0 38.0 75-79 37.43855 38.0 38.0 38.0 37.0 38.0 80-84 37.3697 38.0 38.0 38.0 37.0 38.0 85-89 37.1576 38.0 38.0 38.0 36.4 38.0 90-94 37.21915 38.0 38.0 38.0 36.8 38.0 95-99 37.10515 38.0 38.0 38.0 35.8 38.0 100-104 37.154900000000005 38.0 38.0 38.0 36.0 38.0 105-109 36.989050000000006 38.0 38.0 38.0 35.4 38.0 110-114 36.5042 38.0 38.0 38.0 34.4 38.0 115-119 36.4798 38.0 38.0 38.0 34.4 38.0 120-124 36.54925 38.0 38.0 38.0 34.4 38.0 125-129 36.645300000000006 38.0 38.0 38.0 34.8 38.0 130-134 36.45605 38.0 38.0 38.0 34.0 38.0 135-139 36.2105 38.0 38.0 38.0 33.4 38.0 140-144 36.002 38.0 37.8 38.0 32.8 38.0 145-149 35.6123 38.0 36.6 38.0 31.6 38.0 150-151 31.876625 35.5 32.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 1.0 17 0.0 18 0.0 19 1.0 20 0.0 21 0.0 22 5.0 23 3.0 24 5.0 25 7.0 26 7.0 27 10.0 28 11.0 29 20.0 30 31.0 31 27.0 32 55.0 33 54.0 34 80.0 35 177.0 36 458.0 37 3045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33953735708588 12.097846317468758 6.806700345652753 37.75591597979261 2 22.55563890972743 13.50337584396099 33.9584896224056 29.982495623905976 3 20.724999999999998 19.5 26.775 33.0 4 26.3 26.75 21.6 25.35 5 24.775 31.8 22.925 20.5 6 19.075 32.025 24.0 24.9 7 16.025 22.075 41.825 20.075000000000003 8 17.05 22.3 31.6 29.049999999999997 9 18.75 19.400000000000002 32.525 29.325000000000003 10-14 22.37 26.505000000000003 23.805 27.32 15-19 22.31 24.779999999999998 26.064999999999998 26.845000000000002 20-24 22.61 25.900000000000002 26.095000000000002 25.395 25-29 23.150000000000002 24.21 25.83 26.810000000000002 30-34 22.78 25.66 25.36 26.200000000000003 35-39 22.777277727772777 24.942494249424943 25.962596259625965 26.31763176317632 40-44 22.433973589435773 24.879951980792317 25.910364145658267 26.775710284113647 45-49 21.12 25.080000000000002 27.839999999999996 25.96 50-54 22.338935574229694 24.2547018807523 26.08543417366947 27.32092837134854 55-59 22.07051762940735 25.351337834458615 25.916479119779943 26.66166541635409 60-64 22.27834175126269 24.418662799419913 25.978896834525177 27.32409861479222 65-69 22.525000000000002 24.779999999999998 25.005 27.689999999999998 70-74 23.474999999999998 24.85 24.52 27.155 75-79 22.805 25.645 25.224999999999998 26.325 80-84 23.365 25.169999999999998 25.155 26.31 85-89 22.64 24.385 26.32 26.655 90-94 22.156107805390267 26.126306315315766 24.89624481224061 26.82134106705335 95-99 21.94 25.365 25.03 27.665 100-104 22.977637700735404 25.218870378708292 24.753614487968385 27.04987743258792 105-109 22.99 25.009999999999998 25.419999999999998 26.58 110-114 22.369015924046817 25.297633998091122 26.337469231928466 25.995880845933588 115-119 22.276830491474424 25.792377131394183 24.633901705115345 27.29689067201605 120-124 22.359066786822872 25.818564133373386 23.5656353259237 28.25673375388004 125-129 22.906872061618486 25.932779833950185 24.097229168750626 27.063118935680702 130-134 23.63590897724431 25.98649662415604 23.335833958489623 27.04176044011003 135-139 23.74 25.990000000000002 24.4 25.869999999999997 140-144 23.44 25.674999999999997 24.345 26.540000000000003 145-149 22.78 25.505 24.645 27.07 150-151 21.775 26.2625 24.0625 27.900000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 1.0 22 2.5 23 1.5 24 1.0 25 2.5 26 3.0 27 5.5 28 7.5 29 11.5 30 13.5 31 11.0 32 14.5 33 16.5 34 22.0 35 30.0 36 56.5 37 119.5 38 132.5 39 113.5 40 133.0 41 154.5 42 127.0 43 121.0 44 127.0 45 119.0 46 123.5 47 103.5 48 94.0 49 94.0 50 118.5 51 139.0 52 145.0 53 164.0 54 191.5 55 263.0 56 279.5 57 201.5 58 178.5 59 162.0 60 109.0 61 70.5 62 43.5 63 32.0 64 21.0 65 21.0 66 17.0 67 10.5 68 11.0 69 7.5 70 6.0 71 10.5 72 12.0 73 6.0 74 5.0 75 5.0 76 3.0 77 2.5 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.975 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.01 40-44 0.04 45-49 0.0 50-54 0.04 55-59 0.025 60-64 0.015 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.055 105-109 0.0 110-114 0.46499999999999997 115-119 0.3 120-124 0.13 125-129 0.03 130-134 0.025 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.875 #Duplication Level Percentage of deduplicated Percentage of total 1 81.68157423971377 57.074999999999996 2 9.552772808586763 13.350000000000001 3 3.6493738819320214 7.6499999999999995 4 1.8604651162790697 5.2 5 1.2880143112701252 4.5 6 0.6082289803220036 2.55 7 0.6082289803220036 2.9749999999999996 8 0.17889087656529518 1.0 9 0.2146690518783542 1.35 >10 0.35778175313059035 4.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 43 1.075 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 22 0.5499999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 20 0.5 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 17 0.42500000000000004 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 17 0.42500000000000004 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 13 0.325 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 11 0.27499999999999997 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 11 0.27499999999999997 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 10 0.25 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 10 0.25 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 9 0.22499999999999998 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 9 0.22499999999999998 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 9 0.22499999999999998 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 9 0.22499999999999998 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 9 0.22499999999999998 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 9 0.22499999999999998 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 8 0.2 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 8 0.2 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 7 0.17500000000000002 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 7 0.17500000000000002 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 7 0.17500000000000002 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 7 0.17500000000000002 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 7 0.17500000000000002 No Hit ATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCT 7 0.17500000000000002 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 7 0.17500000000000002 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 7 0.17500000000000002 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 7 0.17500000000000002 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 7 0.17500000000000002 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 7 0.17500000000000002 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 7 0.17500000000000002 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 7 0.17500000000000002 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 6 0.15 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 6 0.15 No Hit CGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGAT 6 0.15 No Hit GTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATC 6 0.15 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 6 0.15 No Hit CACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCT 6 0.15 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 6 0.15 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 6 0.15 No Hit CTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACC 6 0.15 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 6 0.15 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 6 0.15 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 6 0.15 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit AGCACGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCC 6 0.15 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 6 0.15 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit CGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGC 5 0.125 No Hit GTTAGCTACAGCACTGCACGGGTCGAGTCGCACAGCACCTAGTATCCATC 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 5 0.125 No Hit GTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAA 5 0.125 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 5 0.125 No Hit ACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC 5 0.125 No Hit ATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCG 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit CGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 5 0.125 No Hit CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAT 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit GTCGCAAGCCTAGCCTTAGGCATCCCCCTCCTTACGGTTAAGGGTAATGA 5 0.125 No Hit CTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCAT 5 0.125 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 5 0.125 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC 5 0.125 No Hit GCCGACCTTGACCCCTGTTATTTTGGGGTCATATCTAGTATTCAGAGTTT 5 0.125 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 5 0.125 No Hit CAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTGGC 5 0.125 No Hit GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 5 0.125 No Hit GCACCTAGTATCCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCC 5 0.125 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 5 0.125 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 5 0.125 No Hit CCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGC 5 0.125 No Hit CCGCATTAATGGGCGAACAGCCCAACCCTTGGAACCACCTACAGCTCCAG 5 0.125 No Hit CATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGA 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit CTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCC 5 0.125 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 5 0.125 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 5 0.125 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 5 0.125 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 5 0.125 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.32499999999999996 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.55 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.8125 0.0 0.0 0.0 0.0 98-99 1.025 0.0 0.0 0.0 0.0 100-101 1.25 0.0 0.0 0.0 0.0 102-103 1.4625 0.0 0.0 0.0 0.0 104-105 1.6375 0.0 0.0 0.0 0.0 106-107 1.7999999999999998 0.0 0.0 0.0 0.0 108-109 2.0875 0.0 0.0 0.0 0.0 110-111 2.4625000000000004 0.0 0.0 0.0 0.0 112-113 2.9124999999999996 0.0 0.0 0.0 0.0 114-115 3.3 0.0 0.0 0.0 0.0 116-117 3.8875 0.0 0.0 0.0 0.0 118-119 4.3875 0.0 0.0 0.0 0.0 120-121 4.887499999999999 0.0 0.0 0.0 0.0 122-123 5.5625 0.0 0.0 0.0 0.0 124-125 6.0875 0.0 0.0 0.0 0.0 126-127 6.7875 0.0 0.0 0.0 0.0 128-129 7.3125 0.0 0.0 0.0 0.0 130-131 7.95 0.0 0.0 0.0 0.0 132-133 8.8125 0.0 0.0 0.0 0.0 134-135 9.5 0.0 0.0 0.0 0.0 136-137 9.9875 0.0 0.0 0.0 0.0 138-139 10.6125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGTAT 10 0.0068910434 144.575 145 GCTGGAG 10 0.0068910434 144.575 8 CTGGAGT 10 0.0068910434 144.575 9 >>END_MODULE SRR6941609 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941609_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.21575 34.0 33.0 34.0 33.0 34.0 2 33.2355 34.0 33.0 34.0 33.0 34.0 3 33.293 34.0 33.0 34.0 33.0 34.0 4 33.27075 34.0 33.0 34.0 33.0 34.0 5 33.2905 34.0 33.0 34.0 33.0 34.0 6 37.4145 38.0 38.0 38.0 38.0 38.0 7 37.48975 38.0 38.0 38.0 38.0 38.0 8 37.44475 38.0 38.0 38.0 38.0 38.0 9 37.38325 38.0 38.0 38.0 37.0 38.0 10-14 37.4285 38.0 38.0 38.0 38.0 38.0 15-19 37.38699999999999 38.0 38.0 38.0 38.0 38.0 20-24 37.36495000000001 38.0 38.0 38.0 38.0 38.0 25-29 37.26805 38.0 38.0 38.0 37.2 38.0 30-34 37.373000000000005 38.0 38.0 38.0 38.0 38.0 35-39 37.352349999999994 38.0 38.0 38.0 37.6 38.0 40-44 37.3562 38.0 38.0 38.0 37.8 38.0 45-49 37.3412 38.0 38.0 38.0 37.4 38.0 50-54 37.28975 38.0 38.0 38.0 37.2 38.0 55-59 37.26925 38.0 38.0 38.0 37.2 38.0 60-64 37.1514 38.0 38.0 38.0 37.0 38.0 65-69 37.10979999999999 38.0 38.0 38.0 37.0 38.0 70-74 37.138099999999994 38.0 38.0 38.0 37.0 38.0 75-79 37.106500000000004 38.0 38.0 38.0 36.4 38.0 80-84 37.00575 38.0 38.0 38.0 36.2 38.0 85-89 37.00295 38.0 38.0 38.0 36.0 38.0 90-94 36.95504999999999 38.0 38.0 38.0 36.0 38.0 95-99 36.850350000000006 38.0 38.0 38.0 35.6 38.0 100-104 36.5351 38.0 38.0 38.0 34.8 38.0 105-109 36.35195 38.0 38.0 38.0 34.4 38.0 110-114 35.8673 38.0 38.0 38.0 32.8 38.0 115-119 35.7572 38.0 37.2 38.0 32.0 38.0 120-124 35.9199 38.0 37.4 38.0 32.8 38.0 125-129 35.89405000000001 38.0 37.2 38.0 33.0 38.0 130-134 36.020300000000006 38.0 38.0 38.0 33.0 38.0 135-139 35.62215 38.0 36.2 38.0 31.0 38.0 140-144 35.038599999999995 38.0 35.8 38.0 31.0 38.0 145-149 34.09155 38.0 34.2 38.0 25.6 38.0 150-151 29.791375000000002 35.5 27.0 38.0 11.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 1.0 5 2.0 6 1.0 7 1.0 8 3.0 9 2.0 10 1.0 11 0.0 12 0.0 13 0.0 14 2.0 15 2.0 16 2.0 17 0.0 18 5.0 19 3.0 20 8.0 21 3.0 22 2.0 23 3.0 24 6.0 25 8.0 26 14.0 27 23.0 28 15.0 29 22.0 30 32.0 31 41.0 32 58.0 33 62.0 34 131.0 35 208.0 36 531.0 37 2801.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.4 16.975 10.35 24.275 2 30.475 18.65 30.525000000000002 20.349999999999998 3 22.775000000000002 21.275 33.875 22.075 4 26.775 31.275 23.05 18.9 5 29.25 32.35 20.974999999999998 17.424999999999997 6 23.974999999999998 35.175 21.224999999999998 19.625 7 21.65 19.35 36.7 22.3 8 24.325 22.7 26.8 26.174999999999997 9 26.875 23.025000000000002 27.150000000000002 22.95 10-14 27.87 24.610000000000003 24.16 23.36 15-19 27.71 24.85 25.495 21.945 20-24 27.800000000000004 24.325 26.240000000000002 21.634999999999998 25-29 27.128138441532464 25.81774532359708 24.592377713313994 22.46173852155647 30-34 27.376368818440923 26.01130056502825 24.381219060953047 22.23111155557778 35-39 27.054058108716305 26.26393959093864 24.948742311346702 21.73325998899835 40-44 27.38 26.040000000000003 24.88 21.7 45-49 27.155 26.695 24.695 21.455 50-54 27.245 25.14 25.21 22.405 55-59 26.8 25.785000000000004 24.965 22.45 60-64 27.69638481924096 24.586229311465573 25.731286564328215 21.986099304965247 65-69 27.793338001400418 24.852455736721016 25.14254276282885 22.211663499049713 70-74 28.053416024807444 24.93748124437331 25.122536761028307 21.886565969790936 75-79 28.144999999999996 24.425 25.295 22.134999999999998 80-84 27.5320256204964 25.15012009607686 25.830664531625303 21.48718975180144 85-89 28.192048012003003 25.35633908477119 24.431107776944234 22.02050512628157 90-94 27.71 25.575 24.240000000000002 22.475 95-99 28.08 24.275 25.445 22.2 100-104 27.35915140598419 26.628640048033624 24.657260082057437 21.354948463924746 105-109 28.100746081818638 24.720845225577087 25.61714486004707 21.56126383255721 110-114 27.229999999999997 25.080000000000002 25.395 22.295 115-119 28.050000000000004 25.474999999999998 24.455 22.02 120-124 27.55 26.415 23.575 22.46 125-129 27.522752275227525 26.017601760176017 23.85238523852385 22.607260726072607 130-134 27.38732429593317 26.166775048771946 24.260917412835774 22.184983242459104 135-139 27.33273327332733 25.69256925692569 24.372437243724374 22.6022602260226 140-144 28.626450580232092 25.38015206082433 24.59483793517407 21.39855942376951 145-149 27.909536675672967 25.642950065045532 24.64725307715401 21.80026018212749 150-151 29.694541812719077 24.98748122183275 24.161241862794192 21.156735102653982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 0.0 21 1.0 22 2.0 23 3.0 24 3.5 25 4.0 26 6.0 27 7.5 28 7.5 29 10.0 30 16.5 31 17.0 32 17.5 33 26.5 34 33.0 35 37.5 36 54.0 37 81.5 38 96.0 39 120.0 40 139.0 41 127.0 42 117.0 43 144.0 44 157.0 45 117.5 46 114.5 47 119.0 48 102.5 49 95.5 50 99.5 51 123.0 52 126.0 53 142.5 54 221.0 55 267.5 56 222.5 57 160.0 58 149.0 59 148.0 60 122.0 61 100.5 62 85.5 63 58.0 64 34.5 65 23.5 66 15.0 67 20.0 68 21.5 69 14.0 70 9.5 71 10.0 72 8.5 73 8.0 74 9.0 75 6.0 76 5.0 77 4.0 78 2.5 79 1.5 80 1.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.03 30-34 0.005 35-39 0.015 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.005 65-69 0.03 70-74 0.03 75-79 0.0 80-84 0.08 85-89 0.025 90-94 0.0 95-99 0.0 100-104 0.06999999999999999 105-109 0.145 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.01 130-134 0.045 135-139 0.01 140-144 0.04 145-149 0.06999999999999999 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.525 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53973390866594 55.300000000000004 2 11.542610571736784 16.05 3 4.4228694714131604 9.225 4 1.6181229773462782 4.5 5 0.8270406328658756 2.875 6 1.0068320747932398 4.2 7 0.35958288385472853 1.7500000000000002 8 0.0719165767709457 0.4 9 0.0719165767709457 0.44999999999999996 >10 0.5393743257820928 5.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 26 0.65 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 25 0.625 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 17 0.42500000000000004 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 16 0.4 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 14 0.35000000000000003 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 14 0.35000000000000003 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 12 0.3 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 12 0.3 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 12 0.3 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 11 0.27499999999999997 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 11 0.27499999999999997 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 10 0.25 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 10 0.25 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 10 0.25 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 8 0.2 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 7 0.17500000000000002 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 7 0.17500000000000002 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 7 0.17500000000000002 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 7 0.17500000000000002 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 7 0.17500000000000002 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 7 0.17500000000000002 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 7 0.17500000000000002 No Hit GTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGT 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 6 0.15 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 6 0.15 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 6 0.15 No Hit GGCGATTGTCACTGCTTATGGACCCGAACCTGGGTGATCTATCCATGACC 6 0.15 No Hit CTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGC 6 0.15 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit GGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAG 6 0.15 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 6 0.15 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 6 0.15 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 6 0.15 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 6 0.15 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 6 0.15 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 6 0.15 No Hit CAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGT 6 0.15 No Hit CAGAGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCC 6 0.15 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit GGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGA 5 0.125 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 5 0.125 No Hit GGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGC 5 0.125 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 5 0.125 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 5 0.125 No Hit GGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTG 5 0.125 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 5 0.125 No Hit GGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGG 5 0.125 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 5 0.125 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 5 0.125 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 5 0.125 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 5 0.125 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit GTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTG 5 0.125 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 5 0.125 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.32499999999999996 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.55 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.8125 0.0 0.0 0.0 0.0 98-99 1.075 0.0 0.0 0.0 0.0 100-101 1.2999999999999998 0.0 0.0 0.0 0.0 102-103 1.5375 0.0 0.0 0.0 0.0 104-105 1.7 0.0 0.0 0.0 0.0 106-107 1.85 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.5374999999999996 0.0 0.0 0.0 0.0 112-113 3.0125 0.0 0.0 0.0 0.0 114-115 3.4124999999999996 0.0 0.0 0.0 0.0 116-117 3.9749999999999996 0.0 0.0 0.0 0.0 118-119 4.475 0.0 0.0 0.0 0.0 120-121 5.0 0.0 0.0 0.0 0.0 122-123 5.6875 0.0 0.0 0.0 0.0 124-125 6.2375 0.0 0.0 0.0 0.0 126-127 6.9 0.0 0.0 0.0 0.0 128-129 7.4 0.0 0.0 0.0 0.0 130-131 8.024999999999999 0.0 0.0 0.0 0.0 132-133 8.8875 0.0 0.0 0.0 0.0 134-135 9.5625 0.0 0.0 0.0 0.0 136-137 10.037500000000001 0.0 0.0 0.0 0.0 138-139 10.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGTG 10 0.006830828 145.0 145 TTTTTTT 25 4.977651E-4 29.0 55-59 >>END_MODULE Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113197 spots for SRR6941609.sra Written 1113197 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra Read 1113186 spots for SRR6941609.sra Written 1113186 spots for SRR6941609.sra SRR ids: ['SRR6941609.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zzsr2wwp SRR6941609.sra spots: 22263731 blocks: [[1, 1113186], [1113187, 2226372], [2226373, 3339558], [3339559, 4452744], [4452745, 5565930], [5565931, 6679116], [6679117, 7792302], [7792303, 8905488], [8905489, 10018674], [10018675, 11131860], [11131861, 12245046], [12245047, 13358232], [13358233, 14471418], [14471419, 15584604], [15584605, 16697790], [16697791, 17810976], [17810977, 18924162], [18924163, 20037348], [20037349, 21150534], [21150535, 22263731]] SRR6941609 file size 7522747 SRR6941609 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941609 SRR6941609_1.fastq SRR6941609_2.fastq Input file: SRR6941609_1.fastq Paired file: SRR6941609_2.fastq trimmed: SRR6941609-trimmed-pair1.fastq, SRR6941609-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 13:01:05 2024 >> started Fri Dec 6 13:01:32 2024 >> done (26.374s) 22263731 read pairs processed; of these: 13845 ( 0.06%) short read pairs filtered out after trimming by size control 13603 ( 0.06%) empty read pairs filtered out after trimming by size control 22236283 (99.88%) read pairs available; of these: 10770263 (48.44%) trimmed read pairs available after processing 11466020 (51.56%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 2 0.00% 20 2 0.00% 21 3 0.00% 22 4 0.00% 23 3 0.00% 24 7 0.00% 25 7 0.00% 26 8 0.00% 27 8 0.00% 28 13 0.00% 29 4 0.00% 30 9 0.00% 31 7 0.00% 32 12 0.00% 33 14 0.00% 34 15 0.00% 35 20 0.00% 36 12 0.00% 37 17 0.00% 38 21 0.00% 39 28 0.00% 40 24 0.00% 41 30 0.00% 42 25 0.00% 43 33 0.00% 44 36 0.00% 45 39 0.00% 46 44 0.00% 47 50 0.00% 48 63 0.00% 49 79 0.00% 50 91 0.00% 51 114 0.00% 52 142 0.00% 53 148 0.00% 54 166 0.00% 55 165 0.00% 56 189 0.00% 57 251 0.00% 58 246 0.00% 59 324 0.00% 60 321 0.00% 61 414 0.00% 62 509 0.00% 63 562 0.00% 64 606 0.00% 65 740 0.00% 66 743 0.00% 67 899 0.00% 68 1025 0.00% 69 1050 0.00% 70 1284 0.01% 71 1396 0.01% 72 1684 0.01% 73 2006 0.01% 74 2032 0.01% 75 2378 0.01% 76 2750 0.01% 77 3080 0.01% 78 3459 0.02% 79 4134 0.02% 80 4590 0.02% 81 5182 0.02% 82 5582 0.03% 83 6680 0.03% 84 7458 0.03% 85 9136 0.04% 86 9414 0.04% 87 10385 0.05% 88 12410 0.06% 89 12812 0.06% 90 14023 0.06% 91 14940 0.07% 92 17023 0.08% 93 17861 0.08% 94 18663 0.08% 95 21999 0.10% 96 22393 0.10% 97 24909 0.11% 98 25898 0.12% 99 27896 0.13% 100 29014 0.13% 101 32182 0.14% 102 32117 0.14% 103 33305 0.15% 104 36308 0.16% 105 37638 0.17% 106 38541 0.17% 107 41408 0.19% 108 45706 0.21% 109 47119 0.21% 110 47754 0.21% 111 49124 0.22% 112 51396 0.23% 113 50741 0.23% 114 55650 0.25% 115 58536 0.26% 116 60010 0.27% 117 58900 0.26% 118 61798 0.28% 119 61710 0.28% 120 65681 0.30% 121 70202 0.32% 122 74593 0.34% 123 76949 0.35% 124 75845 0.34% 125 81697 0.37% 126 79583 0.36% 127 81871 0.37% 128 81101 0.36% 129 86341 0.39% 130 82662 0.37% 131 87709 0.39% 132 90885 0.41% 133 90193 0.41% 134 94864 0.43% 135 95747 0.43% 136 101718 0.46% 137 101499 0.46% 138 110013 0.49% 139 116009 0.52% 140 118502 0.53% 141 135246 0.61% 142 138711 0.62% 143 151014 0.68% 144 170663 0.77% 145 203444 0.91% 146 236785 1.06% 147 306299 1.38% 148 444303 2.00% 149 861726 3.88% 150 5006648 22.52% 151 11466020 51.56% 22236283 reads passed initial QC criterion=sequence-density sequence-density=1.42 sequence-density-rank=1 fanout-score=3.19 fanout-score-rank=23 prefix-density=2.37 prefix-fanout=1.9 sequence=ATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTGGACTTTAGCCATCTAGGGTGCGGCACTCAACCGCTTCGCCTAGCAGCACAACGCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGTTGACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTTGCTACGCCCTTCCTCGTCTCTGGGTGCCTAGGTATCCACCGCAAGCCTTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=98.22 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=2.6 sequence=CGACTTCACCCCAGTCGAAGACCCCACCGTGGTATGCGCCAATAAGACCACCAAAGGCCTTTGTGGCACTAGTGGTACACAGAAGTCATGGGTGATCATTGGTCCGATGCTTCGGGCGAAACCAATTCCCAGGGTGTGACGGGC criterion=sequence-density sequence-density=0.79 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=32 prefix-density=0.78 prefix-fanout=1.9 sequence=CAAGTGTTGGATT criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=59.45 fanout-score-rank=1 prefix-density=1.04 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941609 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 13:02:19 Started mapping on | Dec 06 13:02:20 Finished on | Dec 06 13:04:08 Mapping speed, Million of reads per hour | 741.21 Number of input reads | 22236283 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 10318053 Uniquely mapped reads % | 46.40% Average mapped length | 294.82 Number of splices: Total | 1344293 Number of splices: Annotated (sjdb) | 1194213 Number of splices: GT/AG | 1265549 Number of splices: GC/AG | 15983 Number of splices: AT/AC | 4386 Number of splices: Non-canonical | 58375 Mismatch rate per base, % | 0.19% Deletion rate per base | 0.01% Deletion average length | 1.70 Insertion rate per base | 0.01% Insertion average length | 1.96 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8227777 % of reads mapped to multiple loci | 37.00% Number of reads mapped to too many loci | 480700 % of reads mapped to too many loci | 2.16% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.08% % of reads unmapped: other | 11.35% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3697378 3697378 3697378 N_multimapping 8227777 8227777 8227777 N_noFeature 6324002 10083350 6421070 N_ambiguous 277760 5447 142560 UnstrandedReadsAssigned:3716291 PositiveStrandReadsAssigned:229256 NegativeStrandReadsAssigned:3754423 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941609 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941609-trimmed-pair1.fastq SRR6941609-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,236,283 reads, 7,480,384 reads pseudoaligned [quant] estimated average fragment length: 213.522 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,031 rounds 52973 SRR6941609.ke.tsv 35125 SRR6941609.se.tsv 88098 total ==> SRR6941609.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 723.871 0 0 PNS24247 1044 831.478 1.51256 0.136825 PNS24249 1928 1715.48 16.4623 0.721788 PNS24246 1044 831.478 1.51256 0.136825 PNS24248 1044 831.478 1.51256 0.136825 PNS24244 1471 1258.48 0 0 PNS24243 293 112.429 0 0 KQK14069 1603 1390.48 280.98 15.199 KQK14071 474 270.127 7.09966 1.97685 ==> SRR6941609.se.tsv <== BRADI_1g14170v3 359 BRADI_1g53295v3 8 BRADI_1g59795v3 17 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 19 BRADI_1g74790v3 18 BRADI_1g09890v3 0 BRADI_1g77505v3 11 BRADI_1g48960v3 0 SRR6941609 completed mapping pipeline successfully