Starting /dee2/code/volunteer_pipeline.sh SRR6941610 current disk space = 1551128182784 free memory = 1602304280 SRR6941610 SRAfilesize 2388ed8377dc8f7dd5f1524d9dc52c61 SRR6941610.sra SRR6941610.sra file validated SRR6941610 is paired end SRR6941610 is conventional basespace SRR6941610 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941610_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.89475 25.0 18.0 31.0 18.0 32.0 2 30.59675 31.0 29.0 33.0 27.0 33.0 3 31.52475 33.0 31.0 33.0 27.0 33.0 4 32.29125 33.0 33.0 33.0 31.0 34.0 5 32.99925 33.0 33.0 34.0 32.0 34.0 6 37.08025 38.0 37.0 38.0 36.0 38.0 7 37.447 38.0 38.0 38.0 37.0 38.0 8 37.573 38.0 38.0 38.0 38.0 38.0 9 37.66625 38.0 38.0 38.0 38.0 38.0 10-14 37.5206 38.0 38.0 38.0 37.8 38.0 15-19 37.3326 38.0 38.0 38.0 36.8 38.0 20-24 37.44735000000001 38.0 38.0 38.0 37.0 38.0 25-29 37.5421 38.0 38.0 38.0 37.8 38.0 30-34 37.56490000000001 38.0 38.0 38.0 38.0 38.0 35-39 37.5452 38.0 38.0 38.0 37.6 38.0 40-44 37.4793 38.0 38.0 38.0 37.4 38.0 45-49 37.459199999999996 38.0 38.0 38.0 37.4 38.0 50-54 37.26205 38.0 38.0 38.0 36.6 38.0 55-59 37.150549999999996 38.0 38.0 38.0 36.0 38.0 60-64 37.2919 38.0 38.0 38.0 36.8 38.0 65-69 37.2291 38.0 38.0 38.0 36.6 38.0 70-74 37.2411 38.0 38.0 38.0 36.2 38.0 75-79 37.196000000000005 38.0 38.0 38.0 36.0 38.0 80-84 37.2803 38.0 38.0 38.0 36.6 38.0 85-89 37.1534 38.0 38.0 38.0 36.2 38.0 90-94 35.88335 38.0 36.6 38.0 29.8 38.0 95-99 36.594849999999994 38.0 37.8 38.0 33.8 38.0 100-104 36.981449999999995 38.0 38.0 38.0 35.6 38.0 105-109 36.846799999999995 38.0 38.0 38.0 35.0 38.0 110-114 36.8112 38.0 38.0 38.0 35.0 38.0 115-119 36.58815 38.0 38.0 38.0 34.4 38.0 120-124 36.5243 38.0 38.0 38.0 34.2 38.0 125-129 36.3093 38.0 38.0 38.0 33.8 38.0 130-134 36.388 38.0 38.0 38.0 33.8 38.0 135-139 36.03589999999999 38.0 37.4 38.0 33.0 38.0 140-144 35.88695 38.0 36.4 38.0 33.0 38.0 145-149 35.3707 38.0 36.0 38.0 31.6 38.0 150-151 32.478875 37.0 33.5 38.0 15.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 2.0 18 0.0 19 3.0 20 2.0 21 2.0 22 4.0 23 0.0 24 4.0 25 6.0 26 8.0 27 10.0 28 12.0 29 21.0 30 33.0 31 38.0 32 62.0 33 105.0 34 146.0 35 238.0 36 616.0 37 2687.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.66712141882674 12.551159618008187 8.10368349249659 31.678035470668487 2 23.411705852926463 15.982991495747875 31.065532766383193 29.539769884942473 3 20.724999999999998 22.650000000000002 26.75 29.875 4 24.875 29.375 22.375 23.375 5 22.875 34.375 23.150000000000002 19.6 6 19.125 35.449999999999996 23.7 21.725 7 14.95 25.3 40.9 18.85 8 18.75 23.5 29.5 28.249999999999996 9 18.05 22.3 31.900000000000002 27.750000000000004 10-14 21.165 29.485 23.885 25.465 15-19 22.314999999999998 26.86 25.635 25.19 20-24 20.89 28.27 26.064999999999998 24.775 25-29 22.865 27.27 25.355 24.51 30-34 22.18 28.73 24.52 24.57 35-39 22.57 27.54 25.759999999999998 24.13 40-44 21.425 26.855 26.229999999999997 25.490000000000002 45-49 21.67 27.595 26.834999999999997 23.9 50-54 21.905 26.55 25.85 25.695 55-59 21.745 27.175 25.624999999999996 25.455 60-64 21.095 27.36 26.625 24.92 65-69 21.665 27.55 25.09 25.695 70-74 22.96 27.66 24.075 25.305 75-79 22.0 27.665 25.115 25.22 80-84 22.975 26.900000000000002 25.11 25.014999999999997 85-89 22.189999999999998 26.889999999999997 26.06 24.86 90-94 21.215 28.560000000000002 24.525 25.7 95-99 21.495 28.275 24.21 26.02 100-104 21.725 28.68 23.91 25.685000000000002 105-109 21.97 27.405 25.5 25.124999999999996 110-114 22.015 27.01 25.585 25.39 115-119 21.905 28.225 24.23 25.64 120-124 21.099999999999998 28.83 22.725 27.345000000000002 125-129 21.965 28.185 23.73 26.119999999999997 130-134 22.54 28.655 22.759999999999998 26.045 135-139 22.85 28.415000000000003 23.775 24.959999999999997 140-144 23.11 28.235 23.945 24.709999999999997 145-149 21.775 27.284999999999997 24.365000000000002 26.575 150-151 21.099999999999998 28.762500000000003 23.425 26.7125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 0.5 20 1.0 21 1.5 22 1.5 23 1.0 24 0.5 25 2.0 26 6.5 27 8.0 28 10.0 29 12.0 30 13.0 31 16.0 32 21.5 33 26.5 34 32.5 35 45.0 36 89.0 37 171.0 38 180.0 39 164.5 40 198.0 41 204.0 42 168.0 43 156.5 44 172.0 45 174.0 46 143.5 47 115.0 48 120.5 49 108.0 50 112.0 51 107.5 52 85.5 53 100.5 54 139.5 55 184.5 56 181.5 57 131.5 58 122.5 59 110.0 60 74.5 61 53.5 62 37.5 63 33.5 64 30.5 65 26.0 66 17.5 67 12.5 68 11.0 69 7.5 70 10.0 71 9.5 72 5.0 73 4.5 74 5.5 75 6.0 76 6.0 77 4.5 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 8.375 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.2 #Duplication Level Percentage of deduplicated Percentage of total 1 83.67275280898876 59.575 2 8.883426966292134 12.65 3 2.8089887640449436 6.0 4 1.6853932584269662 4.8 5 0.9480337078651686 3.375 6 0.526685393258427 2.25 7 0.49157303370786515 2.45 8 0.24578651685393257 1.4000000000000001 9 0.21067415730337077 1.35 >10 0.526685393258427 6.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 42 1.05 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 22 0.5499999999999999 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 22 0.5499999999999999 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 22 0.5499999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 21 0.525 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 15 0.375 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 15 0.375 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 14 0.35000000000000003 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 13 0.325 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 10 0.25 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 10 0.25 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 10 0.25 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 10 0.25 No Hit GTGCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTG 10 0.25 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 10 0.25 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 9 0.22499999999999998 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 9 0.22499999999999998 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 9 0.22499999999999998 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 9 0.22499999999999998 No Hit GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT 9 0.22499999999999998 No Hit GGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTC 9 0.22499999999999998 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 8 0.2 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 8 0.2 No Hit GGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGTGCTA 8 0.2 No Hit GGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGAT 8 0.2 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 8 0.2 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 8 0.2 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 8 0.2 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 7 0.17500000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 7 0.17500000000000002 No Hit GGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTC 7 0.17500000000000002 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 7 0.17500000000000002 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 7 0.17500000000000002 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 7 0.17500000000000002 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 7 0.17500000000000002 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 7 0.17500000000000002 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 7 0.17500000000000002 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 7 0.17500000000000002 No Hit ATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATAT 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 6 0.15 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 6 0.15 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 6 0.15 No Hit GGATAGATCACCCAGGTTCGGGTCCATAAGCAGTGACAATCGCCCTATGA 6 0.15 No Hit TCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTC 6 0.15 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 6 0.15 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 6 0.15 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 6 0.15 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit CATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTAC 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 6 0.15 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 5 0.125 No Hit CCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTA 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 5 0.125 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit TTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAG 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 5 0.125 No Hit CAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAG 5 0.125 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 5 0.125 No Hit GCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAACATGTGAAA 5 0.125 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 5 0.125 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 5 0.125 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 5 0.125 No Hit GCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTG 5 0.125 No Hit GTTCAGGGTTCCAAACTCATAGTGGCAACTAAACACGAGGGTTGCGCTCG 5 0.125 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 5 0.125 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 5 0.125 No Hit GAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAG 5 0.125 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 5 0.125 No Hit CTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAAT 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 5 0.125 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1375 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.2625 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.3875 0.0 0.0 0.0 0.0 82-83 0.48750000000000004 0.0 0.0 0.0 0.0 84-85 0.5874999999999999 0.0 0.0 0.0 0.0 86-87 0.75 0.0 0.0 0.0 0.0 88-89 0.8875 0.0 0.0 0.0 0.0 90-91 1.1375000000000002 0.0 0.0 0.0 0.0 92-93 1.4 0.0 0.0 0.0 0.0 94-95 1.6125 0.0 0.0 0.0 0.0 96-97 1.9749999999999999 0.0 0.0 0.0 0.0 98-99 2.2375 0.0 0.0 0.0 0.0 100-101 2.6 0.0 0.0 0.0 0.0 102-103 3.1 0.0 0.0 0.0 0.0 104-105 3.5125 0.0 0.0 0.0 0.0 106-107 3.9375 0.0 0.0 0.0 0.0 108-109 4.4375 0.0 0.0 0.0 0.0 110-111 5.0125 0.0 0.0 0.0 0.0 112-113 5.475 0.0 0.0 0.0 0.0 114-115 5.9375 0.0 0.0 0.0 0.0 116-117 6.5125 0.0 0.0 0.0 0.0 118-119 7.112500000000001 0.0 0.0 0.0 0.0 120-121 7.7125 0.0 0.0 0.0 0.0 122-123 8.45 0.0 0.0 0.0 0.0 124-125 9.25 0.0 0.0 0.0 0.0 126-127 9.8875 0.0 0.0 0.0 0.0 128-129 10.675 0.0 0.0 0.0 0.0 130-131 11.412500000000001 0.0 0.0 0.0 0.0 132-133 12.0375 0.0 0.0 0.0 0.0 134-135 13.0 0.0 0.0 0.0 0.0 136-137 13.875 0.0 0.0 0.0 0.0 138-139 14.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTTGC 10 0.006841402 144.925 145 GGAAGAG 75 0.0013168867 38.646667 145 AGATCGG 105 8.4627354E-5 12.422144 140-144 >>END_MODULE SRR6941610 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941610_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92225 33.0 33.0 34.0 32.0 34.0 2 33.0365 34.0 33.0 34.0 33.0 34.0 3 33.0615 34.0 33.0 34.0 33.0 34.0 4 33.03425 34.0 33.0 34.0 33.0 34.0 5 32.97325 34.0 33.0 34.0 33.0 34.0 6 37.139 38.0 38.0 38.0 37.0 38.0 7 37.14875 38.0 38.0 38.0 37.0 38.0 8 37.11925 38.0 38.0 38.0 37.0 38.0 9 37.03975 38.0 38.0 38.0 37.0 38.0 10-14 36.7453 38.0 38.0 38.0 35.4 38.0 15-19 36.985 38.0 38.0 38.0 36.6 38.0 20-24 37.095549999999996 38.0 38.0 38.0 37.0 38.0 25-29 37.023900000000005 38.0 38.0 38.0 36.8 38.0 30-34 36.563550000000006 38.0 38.0 38.0 35.2 38.0 35-39 36.54135 38.0 38.0 38.0 34.8 38.0 40-44 36.9464 38.0 38.0 38.0 36.8 38.0 45-49 37.038349999999994 38.0 38.0 38.0 36.8 38.0 50-54 37.004949999999994 38.0 38.0 38.0 37.0 38.0 55-59 36.8829 38.0 38.0 38.0 36.4 38.0 60-64 36.9544 38.0 38.0 38.0 36.4 38.0 65-69 36.831 38.0 38.0 38.0 36.2 38.0 70-74 36.6327 38.0 38.0 38.0 34.8 38.0 75-79 36.77295 38.0 38.0 38.0 36.0 38.0 80-84 36.503 38.0 38.0 38.0 34.6 38.0 85-89 36.2284 38.0 37.8 38.0 33.4 38.0 90-94 36.6798 38.0 38.0 38.0 35.4 38.0 95-99 36.4458 38.0 38.0 38.0 34.4 38.0 100-104 35.5958 38.0 37.0 38.0 30.2 38.0 105-109 35.804300000000005 38.0 37.0 38.0 32.2 38.0 110-114 36.28505 38.0 38.0 38.0 34.0 38.0 115-119 36.3106 38.0 38.0 38.0 34.0 38.0 120-124 35.8902 38.0 37.6 38.0 32.6 38.0 125-129 35.556 38.0 36.4 38.0 31.6 38.0 130-134 35.5646 38.0 36.2 38.0 32.0 38.0 135-139 31.58415 36.4 25.2 38.0 20.2 38.0 140-144 33.325450000000004 37.2 31.2 38.0 24.6 38.0 145-149 34.508050000000004 38.0 35.6 38.0 29.0 38.0 150-151 29.90025 35.5 28.0 38.0 13.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20.0 3 7.0 4 2.0 5 4.0 6 0.0 7 2.0 8 1.0 9 2.0 10 0.0 11 3.0 12 2.0 13 0.0 14 1.0 15 2.0 16 3.0 17 0.0 18 2.0 19 2.0 20 3.0 21 2.0 22 8.0 23 9.0 24 4.0 25 13.0 26 9.0 27 15.0 28 27.0 29 36.0 30 30.0 31 61.0 32 79.0 33 98.0 34 156.0 35 303.0 36 729.0 37 2365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.949999999999996 17.125 11.35 20.575 2 30.773079809857396 16.86264698523893 31.39854891168376 20.965724293219914 3 22.183274912368553 21.832749123685527 35.25287931897847 20.73109664496745 4 26.126126126126124 30.53053053053053 24.74974974974975 18.593593593593592 5 26.95195195195195 32.63263263263263 22.52252252252252 17.892892892892892 6 22.45 35.05 23.474999999999998 19.025 7 19.475 19.0 40.025 21.5 8 23.175 20.849999999999998 28.549999999999997 27.425 9 23.0 22.650000000000002 30.0 24.349999999999998 10-14 26.450000000000003 24.535 27.48 21.535 15-19 25.569999999999997 24.965 28.23 21.235 20-24 26.029999999999998 24.255 28.345 21.37 25-29 26.17761776177618 25.14251425142514 26.86768676867687 21.81218121812181 30-34 26.390278055611123 24.6999399879976 28.065613122624526 20.844168833766755 35-39 26.00630031501575 24.996249812490625 27.336366818340917 21.66108305415271 40-44 25.6 25.929999999999996 27.205000000000002 21.265 45-49 25.53510702140428 26.16023204640928 26.59531906381276 21.709341868373674 50-54 25.92759275927593 24.56745674567457 27.88278827882788 21.62216221622162 55-59 25.39 25.36 27.275 21.975 60-64 25.412541254125415 24.887488748874887 27.662766276627664 22.037203720372037 65-69 26.29288786635991 24.667400220066018 27.853356006802038 21.186355906772032 70-74 26.346586646661663 24.38609652413103 27.396849212303074 21.870467616904225 75-79 26.621331066553328 24.47122356117806 27.191359567978402 21.716085804290213 80-84 25.82258225822582 24.57245724572457 27.85778577857786 21.747174717471747 85-89 26.25 24.945 26.66 22.145 90-94 25.405 25.415 26.435 22.745 95-99 26.055 24.425 27.735 21.785 100-104 26.015 25.045 28.09 20.849999999999998 105-109 27.355 24.21 27.334999999999997 21.099999999999998 110-114 25.74757475747575 25.032503250325032 27.61776177617762 21.602160216021602 115-119 27.09270927092709 24.917491749174918 26.762676267626762 21.227122712271225 120-124 26.33763376337634 26.147614761476145 25.802580258025802 21.71217121712171 125-129 25.816290814540725 26.97134856742837 25.806290314515728 21.406070303515175 130-134 26.57 26.13 26.26 21.04 135-139 26.224999999999998 26.150000000000002 26.77 20.855 140-144 27.78138906945347 26.01630081504075 26.856342817140856 19.34596729836492 145-149 26.905 26.36 26.479999999999997 20.255000000000003 150-151 27.6875 25.25 26.6 20.4625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 2.5 23 2.5 24 3.0 25 7.5 26 9.0 27 10.0 28 12.5 29 14.5 30 16.0 31 18.0 32 20.5 33 26.0 34 39.0 35 46.5 36 68.0 37 113.0 38 145.5 39 170.0 40 199.0 41 190.0 42 157.0 43 173.5 44 191.0 45 165.0 46 151.5 47 131.0 48 108.5 49 99.0 50 96.0 51 101.5 52 79.0 53 98.0 54 159.0 55 181.0 56 154.0 57 115.5 58 118.5 59 127.0 60 99.0 61 72.5 62 64.5 63 48.0 64 30.0 65 20.0 66 14.5 67 17.5 68 23.0 69 17.5 70 10.5 71 10.5 72 9.5 73 6.5 74 3.0 75 2.5 76 6.0 77 9.0 78 7.0 79 2.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.15 4 0.1 5 0.1 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.01 30-34 0.02 35-39 0.005 40-44 0.0 45-49 0.02 50-54 0.01 55-59 0.0 60-64 0.01 65-69 0.03 70-74 0.025 75-79 0.005 80-84 0.01 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.01 115-119 0.01 120-124 0.01 125-129 0.005 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.95 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21827411167513 56.00000000000001 2 9.572153734590284 13.200000000000001 3 4.894851341551849 10.125 4 1.4503263234227701 4.0 5 0.9427121102248005 3.25 6 0.47135605511240025 1.95 7 0.36258158085569253 1.7500000000000002 8 0.25380710659898476 1.4000000000000001 9 0.10877447425670776 0.675 >10 0.7251631617113851 7.6499999999999995 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 25 0.625 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 23 0.575 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 21 0.525 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 20 0.5 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 20 0.5 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 19 0.475 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 17 0.42500000000000004 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 17 0.42500000000000004 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 15 0.375 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 14 0.35000000000000003 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 13 0.325 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 13 0.325 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 12 0.3 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 12 0.3 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 12 0.3 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 11 0.27499999999999997 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 11 0.27499999999999997 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 11 0.27499999999999997 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 10 0.25 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 10 0.25 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 9 0.22499999999999998 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 9 0.22499999999999998 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit ATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGAC 8 0.2 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 8 0.2 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 8 0.2 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 8 0.2 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 8 0.2 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 7 0.17500000000000002 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 7 0.17500000000000002 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 7 0.17500000000000002 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 7 0.17500000000000002 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 7 0.17500000000000002 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit GCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACAC 7 0.17500000000000002 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 6 0.15 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 6 0.15 No Hit GTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGA 6 0.15 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 6 0.15 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 6 0.15 No Hit GGGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTT 6 0.15 No Hit GCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGG 6 0.15 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 6 0.15 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 5 0.125 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 5 0.125 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 5 0.125 No Hit AGAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTG 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 5 0.125 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 5 0.125 No Hit GTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCT 5 0.125 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit CCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGG 5 0.125 No Hit GTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATG 5 0.125 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 5 0.125 No Hit GACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCC 5 0.125 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 5 0.125 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 5 0.125 No Hit GCCTGTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTA 5 0.125 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 5 0.125 No Hit GGGTAGAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGCG 5 0.125 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 5 0.125 No Hit GGCTTTTCAAGTCCGCCGTCAAATCCCAGGGCTCAACCCTGGACAGGCGG 5 0.125 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1375 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.2625 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.3875 0.0 0.0 0.0 0.0 82-83 0.48750000000000004 0.0 0.0 0.0 0.0 84-85 0.5874999999999999 0.0 0.0 0.0 0.0 86-87 0.75 0.0 0.0 0.0 0.0 88-89 0.925 0.0 0.0 0.0 0.0 90-91 1.1625 0.0 0.0 0.0 0.0 92-93 1.425 0.0 0.0 0.0 0.0 94-95 1.6375 0.0 0.0 0.0 0.0 96-97 1.9375 0.0 0.0 0.0 0.0 98-99 2.1375 0.0 0.0 0.0 0.0 100-101 2.5 0.0 0.0 0.0 0.0 102-103 2.925 0.0 0.0 0.0 0.0 104-105 3.2874999999999996 0.0 0.0 0.0 0.0 106-107 3.7125000000000004 0.0 0.0 0.0 0.0 108-109 4.2 0.0 0.0 0.0 0.0 110-111 4.7625 0.0 0.0 0.0 0.0 112-113 5.225 0.0 0.0 0.0 0.0 114-115 5.675000000000001 0.0 0.0 0.0 0.0 116-117 6.25 0.0 0.0 0.0 0.0 118-119 6.9125 0.0 0.0 0.0 0.0 120-121 7.5125 0.0 0.0 0.0 0.0 122-123 8.225000000000001 0.0 0.0 0.0 0.0 124-125 8.975 0.0 0.0 0.0 0.0 126-127 9.5125 0.0 0.0 0.0 0.0 128-129 10.100000000000001 0.0 0.0 0.0 0.0 130-131 10.625 0.0 0.0 0.0 0.0 132-133 11.0625 0.0 0.0 0.0 0.0 134-135 11.7375 0.0 0.0 0.0 0.0 136-137 12.45 0.0 0.0 0.0 0.0 138-139 13.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAAGAG 100 0.005388326 29.0 145 GATCGGA 105 0.0011959262 13.809524 145 AGATCGG 105 5.384163E-6 13.809524 140-144 ATCGGAA 95 0.007278115 10.684211 140-144 >>END_MODULE Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206466 spots for SRR6941610.sra Written 1206466 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra Read 1206456 spots for SRR6941610.sra Written 1206456 spots for SRR6941610.sra SRR ids: ['SRR6941610.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cnzrtdwz SRR6941610.sra spots: 24129130 blocks: [[1, 1206456], [1206457, 2412912], [2412913, 3619368], [3619369, 4825824], [4825825, 6032280], [6032281, 7238736], [7238737, 8445192], [8445193, 9651648], [9651649, 10858104], [10858105, 12064560], [12064561, 13271016], [13271017, 14477472], [14477473, 15683928], [15683929, 16890384], [16890385, 18096840], [18096841, 19303296], [19303297, 20509752], [20509753, 21716208], [21716209, 22922664], [22922665, 24129130]] SRR6941610 file size 8154870 SRR6941610 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941610 SRR6941610_1.fastq SRR6941610_2.fastq Input file: SRR6941610_1.fastq Paired file: SRR6941610_2.fastq trimmed: SRR6941610-trimmed-pair1.fastq, SRR6941610-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 13:05:08 2024 >> started Fri Dec 6 13:05:40 2024 >> done (31.681s) 24129130 read pairs processed; of these: 52901 ( 0.22%) short read pairs filtered out after trimming by size control 39079 ( 0.16%) empty read pairs filtered out after trimming by size control 24037150 (99.62%) read pairs available; of these: 10646304 (44.29%) trimmed read pairs available after processing 13390846 (55.71%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 0 0.00% 20 3 0.00% 21 2 0.00% 22 4 0.00% 23 4 0.00% 24 2 0.00% 25 8 0.00% 26 10 0.00% 27 43 0.00% 28 9 0.00% 29 8 0.00% 30 5 0.00% 31 10 0.00% 32 14 0.00% 33 8 0.00% 34 16 0.00% 35 12 0.00% 36 17 0.00% 37 10 0.00% 38 33 0.00% 39 26 0.00% 40 33 0.00% 41 44 0.00% 42 48 0.00% 43 53 0.00% 44 41 0.00% 45 62 0.00% 46 81 0.00% 47 101 0.00% 48 109 0.00% 49 134 0.00% 50 186 0.00% 51 211 0.00% 52 276 0.00% 53 275 0.00% 54 308 0.00% 55 404 0.00% 56 392 0.00% 57 471 0.00% 58 556 0.00% 59 620 0.00% 60 790 0.00% 61 959 0.00% 62 1254 0.01% 63 1372 0.01% 64 1606 0.01% 65 1696 0.01% 66 1893 0.01% 67 2104 0.01% 68 2516 0.01% 69 2799 0.01% 70 3215 0.01% 71 3716 0.02% 72 4582 0.02% 73 5160 0.02% 74 5448 0.02% 75 6218 0.03% 76 7010 0.03% 77 7633 0.03% 78 8610 0.04% 79 9930 0.04% 80 11359 0.05% 81 12696 0.05% 82 14069 0.06% 83 15859 0.07% 84 18563 0.08% 85 22386 0.09% 86 23079 0.10% 87 24752 0.10% 88 28412 0.12% 89 28827 0.12% 90 31233 0.13% 91 31223 0.13% 92 36669 0.15% 93 37099 0.15% 94 39557 0.16% 95 43837 0.18% 96 42438 0.18% 97 44508 0.19% 98 46560 0.19% 99 48995 0.20% 100 50942 0.21% 101 53302 0.22% 102 55796 0.23% 103 55576 0.23% 104 60575 0.25% 105 61654 0.26% 106 60937 0.25% 107 64627 0.27% 108 69810 0.29% 109 68662 0.29% 110 71308 0.30% 111 72907 0.30% 112 76366 0.32% 113 74933 0.31% 114 86114 0.36% 115 88242 0.37% 116 88849 0.37% 117 86359 0.36% 118 86744 0.36% 119 84750 0.35% 120 87551 0.36% 121 92823 0.39% 122 108883 0.45% 123 106635 0.44% 124 106402 0.44% 125 111646 0.46% 126 105419 0.44% 127 108853 0.45% 128 105955 0.44% 129 112564 0.47% 130 103409 0.43% 131 111514 0.46% 132 117914 0.49% 133 110479 0.46% 134 120191 0.50% 135 114752 0.48% 136 119420 0.50% 137 117270 0.49% 138 129327 0.54% 139 130048 0.54% 140 129520 0.54% 141 150730 0.63% 142 143092 0.60% 143 151581 0.63% 144 161680 0.67% 145 191012 0.79% 146 207358 0.86% 147 244281 1.02% 148 340389 1.42% 149 615317 2.56% 150 3986551 16.58% 151 13390846 55.71% 24037150 reads passed initial QC criterion=sequence-density sequence-density=1.64 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=28 prefix-density=1.62 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=146.62 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=6.4 sequence=TTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTTCGTTACGAGCTTGTACACAGGCTTCTAAAGCCACTCGATTAGCTGCTGCACCAGGTGCATTTCCCCAAGGATGTCCTAAAGTTCCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAAGCTACTGGTATAACACCTGGCATGGATACCCAGTCCTGAGTGAAAAAGATACCGCGAGCACGATCTTTTTCAATAAAATCGTCGCGCAATAAATCAACAAAACCTAAAGTGATTTCGCGTTCCCCTTCTAACTTACCTACTACTGTACCGGCGTGGATATG criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=30 prefix-density=0.43 prefix-fanout=2.3 sequence=ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=45 fanout-score=665.00 fanout-score-rank=1 prefix-density=3.71 prefix-fanout=1.0 sequence=AAGTAATGCAACTATGAATCTCATGGAGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAGGAGGGGCTCGCGTCTGATTAGCTAGTTGGTGAGGCAATAGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAGAGGATGC SRR6941610 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 13:06:22 Started mapping on | Dec 06 13:06:22 Finished on | Dec 06 13:08:53 Mapping speed, Million of reads per hour | 573.07 Number of input reads | 24037150 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 12800892 Uniquely mapped reads % | 53.25% Average mapped length | 291.69 Number of splices: Total | 2270155 Number of splices: Annotated (sjdb) | 2027377 Number of splices: GT/AG | 2140142 Number of splices: GC/AG | 27604 Number of splices: AT/AC | 7361 Number of splices: Non-canonical | 95048 Mismatch rate per base, % | 0.29% Deletion rate per base | 0.02% Deletion average length | 1.98 Insertion rate per base | 0.02% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8067200 % of reads mapped to multiple loci | 33.56% Number of reads mapped to too many loci | 353721 % of reads mapped to too many loci | 1.47% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.58% % of reads unmapped: other | 7.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3189069 3189069 3189069 N_multimapping 8067200 8067200 8067200 N_noFeature 6344981 12449930 6504843 N_ambiguous 367312 4068 180251 UnstrandedReadsAssigned:6088599 PositiveStrandReadsAssigned:346894 NegativeStrandReadsAssigned:6115798 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR6941610 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941610-trimmed-pair1.fastq SRR6941610-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,037,150 reads, 10,226,195 reads pseudoaligned [quant] estimated average fragment length: 206.518 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,050 rounds 52973 SRR6941610.ke.tsv 35125 SRR6941610.se.tsv 88098 total ==> SRR6941610.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 730.821 0 0 PNS24247 1044 838.482 16.5252 1.53217 PNS24249 1928 1722.48 25.6405 1.15725 PNS24246 1044 838.482 16.5252 1.53217 PNS24248 1044 838.482 16.5252 1.53217 PNS24244 1471 1265.48 11.7839 0.723915 PNS24243 293 120.072 0 0 KQK14069 1603 1397.48 3029.44 168.527 KQK14071 474 277.923 87.9577 24.6038 ==> SRR6941610.se.tsv <== BRADI_1g14170v3 3697 BRADI_1g53295v3 43 BRADI_1g59795v3 65 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 53 BRADI_1g74790v3 8 BRADI_1g09890v3 0 BRADI_1g77505v3 62 BRADI_1g48960v3 0 SRR6941610 completed mapping pipeline successfully