Starting /dee2/code/volunteer_pipeline.sh SRR6941611 current disk space = 1551221907456 free memory = 1327933040 SRR6941611 SRAfilesize 06bc29829f19ff4820e70f110a4fc1ff SRR6941611.sra SRR6941611.sra file validated SRR6941611 is paired end SRR6941611 is conventional basespace SRR6941611 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941611_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61025 34.0 33.0 34.0 32.0 34.0 2 32.94 34.0 33.0 34.0 31.0 34.0 3 33.0715 34.0 33.0 34.0 32.0 34.0 4 33.3405 34.0 33.0 34.0 32.0 34.0 5 33.353 34.0 33.0 34.0 33.0 34.0 6 37.18525 38.0 38.0 38.0 36.0 38.0 7 37.524 38.0 38.0 38.0 37.0 38.0 8 37.615 38.0 38.0 38.0 38.0 38.0 9 37.63325 38.0 38.0 38.0 38.0 38.0 10-14 37.62975 38.0 38.0 38.0 38.0 38.0 15-19 37.5932 38.0 38.0 38.0 38.0 38.0 20-24 37.59895 38.0 38.0 38.0 38.0 38.0 25-29 37.601350000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.51475 38.0 38.0 38.0 38.0 38.0 35-39 37.562850000000005 38.0 38.0 38.0 38.0 38.0 40-44 37.54095 38.0 38.0 38.0 38.0 38.0 45-49 37.4681 38.0 38.0 38.0 38.0 38.0 50-54 37.5043 38.0 38.0 38.0 38.0 38.0 55-59 37.48875 38.0 38.0 38.0 37.8 38.0 60-64 37.4636 38.0 38.0 38.0 37.8 38.0 65-69 37.40195 38.0 38.0 38.0 37.2 38.0 70-74 37.272349999999996 38.0 38.0 38.0 37.0 38.0 75-79 37.313199999999995 38.0 38.0 38.0 36.8 38.0 80-84 37.32684999999999 38.0 38.0 38.0 37.0 38.0 85-89 37.24505 38.0 38.0 38.0 37.0 38.0 90-94 37.16375 38.0 38.0 38.0 36.4 38.0 95-99 37.117399999999996 38.0 38.0 38.0 36.0 38.0 100-104 37.1228 38.0 38.0 38.0 36.0 38.0 105-109 37.019349999999996 38.0 38.0 38.0 35.6 38.0 110-114 36.737 38.0 38.0 38.0 35.0 38.0 115-119 36.7059 38.0 38.0 38.0 35.0 38.0 120-124 36.806349999999995 38.0 38.0 38.0 35.0 38.0 125-129 36.6981 38.0 38.0 38.0 34.8 38.0 130-134 36.5369 38.0 38.0 38.0 34.2 38.0 135-139 36.3631 38.0 38.0 38.0 33.8 38.0 140-144 36.2002 38.0 38.0 38.0 33.6 38.0 145-149 36.01350000000001 38.0 38.0 38.0 33.0 38.0 150-151 32.23525 35.5 32.0 38.0 17.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 1.0 13 0.0 14 1.0 15 1.0 16 0.0 17 1.0 18 1.0 19 1.0 20 0.0 21 0.0 22 1.0 23 3.0 24 3.0 25 6.0 26 8.0 27 5.0 28 19.0 29 19.0 30 22.0 31 31.0 32 40.0 33 68.0 34 98.0 35 157.0 36 392.0 37 3120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.059149722735675 11.909162925798785 5.9941906522313175 34.03749669923422 2 23.525 12.225 32.875 31.374999999999996 3 20.375 19.425 26.575 33.625 4 24.65 28.225 22.35 24.775 5 25.174999999999997 32.65 20.775 21.4 6 19.325 32.45 23.65 24.575 7 16.2 21.925 41.525 20.349999999999998 8 19.425 20.75 30.075000000000003 29.75 9 18.3 21.0 31.874999999999996 28.825 10-14 22.255 25.71 24.625 27.41 15-19 22.86 24.485 25.525 27.13 20-24 22.605 24.47 26.119999999999997 26.805 25-29 23.09 24.38 25.735000000000003 26.795 30-34 22.939999999999998 25.5 24.965 26.595000000000002 35-39 22.455 24.995 26.205000000000002 26.345000000000002 40-44 22.71 23.990000000000002 26.07 27.229999999999997 45-49 22.16110805540277 24.426221311065554 26.936346817340866 26.47632381619081 50-54 22.37 24.175 25.490000000000002 27.965 55-59 22.0 24.665 26.200000000000003 27.134999999999998 60-64 22.02 24.065 26.765 27.150000000000002 65-69 22.56 24.605 25.705 27.13 70-74 23.32 25.03 24.3 27.35 75-79 22.93 25.074999999999996 25.5 26.495 80-84 22.985 24.69 25.490000000000002 26.834999999999997 85-89 22.935 24.275 25.525 27.265 90-94 22.66 25.03 24.740000000000002 27.57 95-99 22.52 25.369999999999997 25.165 26.945000000000004 100-104 22.78569642410603 25.516379094773693 24.79619904976244 26.901725431357836 105-109 22.715 24.515 25.990000000000002 26.779999999999998 110-114 22.330876084449123 25.139160523544458 25.089012587132036 27.44095080487438 115-119 22.43374580431842 25.725164069936373 24.597966033765843 27.24312409197936 120-124 22.67 25.509999999999998 23.66 28.16 125-129 23.24 25.34 23.665 27.755000000000003 130-134 23.599999999999998 25.36 23.855 27.185 135-139 23.14 26.035000000000004 24.095 26.729999999999997 140-144 23.585 26.14 23.845 26.43 145-149 22.53 25.480000000000004 24.82 27.169999999999998 150-151 21.9 26.687499999999996 23.825 27.5875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.5 22 3.5 23 4.5 24 2.5 25 2.0 26 8.0 27 11.0 28 8.0 29 6.5 30 11.5 31 15.0 32 14.0 33 16.5 34 20.0 35 27.5 36 62.0 37 107.0 38 102.0 39 90.5 40 122.0 41 133.0 42 112.0 43 120.0 44 129.0 45 122.0 46 109.5 47 97.0 48 103.0 49 105.5 50 117.5 51 138.0 52 147.0 53 158.0 54 195.0 55 279.0 56 285.0 57 208.5 58 197.0 59 175.5 60 113.5 61 77.5 62 52.0 63 37.0 64 30.0 65 19.5 66 13.5 67 12.0 68 10.5 69 7.0 70 7.5 71 9.0 72 12.0 73 11.0 74 4.0 75 3.0 76 4.5 77 2.5 78 2.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.005 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.025 105-109 0.0 110-114 0.295 115-119 0.19499999999999998 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.65 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21019108280255 57.375 2 9.660297239915074 13.65 3 4.847841472045293 10.274999999999999 4 1.6631280962491155 4.7 5 0.9200283085633405 3.25 6 0.5661712668082095 2.4 7 0.28308563340410475 1.4000000000000001 8 0.17692852087756544 1.0 9 0.17692852087756544 1.125 >10 0.4953998584571833 4.825 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 26 0.65 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 17 0.42500000000000004 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 17 0.42500000000000004 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 16 0.4 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 15 0.375 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 14 0.35000000000000003 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 13 0.325 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 13 0.325 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 11 0.27499999999999997 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 11 0.27499999999999997 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 10 0.25 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 10 0.25 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 10 0.25 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 10 0.25 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 9 0.22499999999999998 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 9 0.22499999999999998 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 9 0.22499999999999998 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 9 0.22499999999999998 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 9 0.22499999999999998 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 8 0.2 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 8 0.2 No Hit GTCGCCCAGGGCATAAGGGGCATGATGACTTGGCCTCATCCTCTCCTTCC 8 0.2 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 8 0.2 No Hit GTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGC 8 0.2 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 7 0.17500000000000002 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit GGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGG 7 0.17500000000000002 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 7 0.17500000000000002 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 7 0.17500000000000002 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 7 0.17500000000000002 No Hit GGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTG 7 0.17500000000000002 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 6 0.15 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 6 0.15 No Hit CCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGA 6 0.15 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 6 0.15 No Hit ACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAAC 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 6 0.15 No Hit CCTTGGGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAAC 6 0.15 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit GCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCTCCTTTTGCTCCTC 6 0.15 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 6 0.15 No Hit CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG 6 0.15 No Hit GGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCCTG 6 0.15 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 6 0.15 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 6 0.15 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 6 0.15 No Hit CCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTG 5 0.125 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CCTTGGCCCGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGT 5 0.125 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 5 0.125 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 5 0.125 No Hit GTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCA 5 0.125 No Hit GAGCACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTAT 5 0.125 No Hit CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAT 5 0.125 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 5 0.125 No Hit AGGCGGGATACTTAACGCGTTAGCTACAGCACTGCACGGGTCGAGTCGCA 5 0.125 No Hit CAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTGGC 5 0.125 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 5 0.125 No Hit GTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGA 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit CCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGC 5 0.125 No Hit GCCTGCAATCCGAACTGAGGACGGGTTTTTGGAGTTAGCTCACCCTCGCG 5 0.125 No Hit GTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCA 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 5 0.125 No Hit GCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGG 5 0.125 No Hit GTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCA 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 5 0.125 No Hit CCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGA 5 0.125 No Hit GTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGTTGACC 5 0.125 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.0875 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.1375 0.0 0.0 0.0 0.0 86-87 0.2375 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.5 0.0 0.0 0.0 0.0 94-95 0.6375 0.0 0.0 0.0 0.0 96-97 0.8125 0.0 0.0 0.0 0.0 98-99 0.975 0.0 0.0 0.0 0.0 100-101 1.1875 0.0 0.0 0.0 0.0 102-103 1.6625 0.0 0.0 0.0 0.0 104-105 1.9125 0.0 0.0 0.0 0.0 106-107 2.175 0.0 0.0 0.0 0.0 108-109 2.4625 0.0 0.0 0.0 0.0 110-111 2.825 0.0 0.0 0.0 0.0 112-113 3.35 0.0 0.0 0.0 0.0 114-115 3.975 0.0 0.0 0.0 0.0 116-117 4.449999999999999 0.0 0.0 0.0 0.0 118-119 4.949999999999999 0.0 0.0 0.0 0.0 120-121 5.4125 0.0 0.0 0.0 0.0 122-123 5.9375 0.0 0.0 0.0 0.0 124-125 6.6375 0.0 0.0 0.0 0.0 126-127 7.1875 0.0 0.0 0.0 0.0 128-129 7.699999999999999 0.0 0.0 0.0 0.0 130-131 8.425 0.0 0.0 0.0 0.0 132-133 9.075 0.0 0.0 0.0 0.0 134-135 9.8375 0.0 0.0 0.0 0.0 136-137 10.7 0.0 0.0 0.0 0.0 138-139 11.45 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCGTC 10 0.006836113 144.9625 145 >>END_MODULE SRR6941611 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941611_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.1265 34.0 33.0 34.0 33.0 34.0 2 33.28775 34.0 33.0 34.0 33.0 34.0 3 33.28525 34.0 33.0 34.0 33.0 34.0 4 33.20875 34.0 33.0 34.0 33.0 34.0 5 33.2775 34.0 33.0 34.0 33.0 34.0 6 37.4345 38.0 38.0 38.0 38.0 38.0 7 37.46525 38.0 38.0 38.0 38.0 38.0 8 37.445 38.0 38.0 38.0 38.0 38.0 9 37.4445 38.0 38.0 38.0 38.0 38.0 10-14 37.406400000000005 38.0 38.0 38.0 38.0 38.0 15-19 37.39235 38.0 38.0 38.0 38.0 38.0 20-24 37.339749999999995 38.0 38.0 38.0 37.8 38.0 25-29 37.34025 38.0 38.0 38.0 37.8 38.0 30-34 37.34505 38.0 38.0 38.0 37.8 38.0 35-39 37.3238 38.0 38.0 38.0 37.6 38.0 40-44 37.3073 38.0 38.0 38.0 37.4 38.0 45-49 37.3344 38.0 38.0 38.0 37.8 38.0 50-54 37.266549999999995 38.0 38.0 38.0 37.4 38.0 55-59 37.252050000000004 38.0 38.0 38.0 37.0 38.0 60-64 37.142700000000005 38.0 38.0 38.0 37.0 38.0 65-69 37.155049999999996 38.0 38.0 38.0 37.0 38.0 70-74 37.11215 38.0 38.0 38.0 36.6 38.0 75-79 37.059799999999996 38.0 38.0 38.0 36.8 38.0 80-84 37.0993 38.0 38.0 38.0 36.6 38.0 85-89 37.0322 38.0 38.0 38.0 36.0 38.0 90-94 36.962650000000004 38.0 38.0 38.0 35.8 38.0 95-99 36.820049999999995 38.0 38.0 38.0 35.0 38.0 100-104 36.76755 38.0 38.0 38.0 35.0 38.0 105-109 36.54745 38.0 38.0 38.0 34.4 38.0 110-114 36.168150000000004 38.0 38.0 38.0 33.6 38.0 115-119 36.05694999999999 38.0 37.8 38.0 33.4 38.0 120-124 36.10545 38.0 38.0 38.0 33.6 38.0 125-129 36.1235 38.0 38.0 38.0 33.4 38.0 130-134 36.007400000000004 38.0 38.0 38.0 32.8 38.0 135-139 35.77565 38.0 37.8 38.0 31.8 38.0 140-144 35.4499 38.0 36.0 38.0 31.0 38.0 145-149 34.5647 38.0 35.8 38.0 28.2 38.0 150-151 29.7475 35.5 27.0 38.0 12.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 0.0 5 0.0 6 1.0 7 2.0 8 0.0 9 3.0 10 0.0 11 1.0 12 0.0 13 2.0 14 0.0 15 2.0 16 0.0 17 1.0 18 4.0 19 2.0 20 3.0 21 2.0 22 4.0 23 6.0 24 7.0 25 7.0 26 13.0 27 18.0 28 15.0 29 25.0 30 37.0 31 36.0 32 55.0 33 78.0 34 119.0 35 192.0 36 477.0 37 2879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.325 18.975 9.049999999999999 22.650000000000002 2 30.0 19.1 29.65 21.25 3 24.0 21.175 33.925 20.9 4 26.674999999999997 31.45 22.225 19.650000000000002 5 29.599999999999998 32.775 19.6 18.025 6 25.775 33.225 20.45 20.549999999999997 7 22.725 19.35 36.375 21.55 8 24.175 21.45 25.0 29.375 9 26.05 22.45 26.474999999999998 25.025 10-14 28.439999999999998 24.415 24.015 23.13 15-19 27.61 25.635 24.695 22.06 20-24 27.815 24.63 25.295 22.259999999999998 25-29 27.375 25.71 24.385 22.53 30-34 27.825 25.885 24.5 21.790000000000003 35-39 27.26 26.479999999999997 24.515 21.745 40-44 27.43 26.32 24.325 21.925 45-49 27.339999999999996 26.700000000000003 23.9 22.06 50-54 27.605 25.380000000000003 24.465 22.55 55-59 26.88 26.009999999999998 24.654999999999998 22.455 60-64 27.950000000000003 25.25 25.014999999999997 21.785 65-69 28.185 25.15 24.19 22.475 70-74 28.525 24.665 25.0 21.81 75-79 28.235 24.635 24.675 22.455 80-84 27.97 24.925 24.625 22.48 85-89 27.71 25.44 24.13 22.720000000000002 90-94 27.889999999999997 25.56 23.66 22.89 95-99 26.77 25.25 25.165 22.814999999999998 100-104 27.735 25.97 24.59 21.705 105-109 28.691434571728585 23.771188559427973 25.796289814490724 21.741087054352718 110-114 27.35636781839092 26.09630481524076 24.606230311515574 21.941097054852744 115-119 28.29 25.605 24.515 21.59 120-124 27.689999999999998 26.375 23.465 22.470000000000002 125-129 27.87 26.05 23.305 22.775000000000002 130-134 27.98 26.375 23.735 21.91 135-139 27.805000000000003 25.525 24.435000000000002 22.235 140-144 29.146457322866144 25.36126806340317 24.46122306115306 21.03105155257763 145-149 28.634295144271643 26.929039355903384 23.678551782767414 20.758113717057558 150-151 29.889944972486244 26.013006503251624 23.54927463731866 20.54777388694347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 2.0 23 2.0 24 1.5 25 2.0 26 5.0 27 9.5 28 9.5 29 9.0 30 15.5 31 19.0 32 14.5 33 21.5 34 39.5 35 50.5 36 57.0 37 76.0 38 91.5 39 93.0 40 115.5 41 125.5 42 116.5 43 135.5 44 143.0 45 120.5 46 107.5 47 95.0 48 90.0 49 100.0 50 99.0 51 108.0 52 109.5 53 147.5 54 231.5 55 267.5 56 254.5 57 199.0 58 167.0 59 172.0 60 141.0 61 95.5 62 76.0 63 59.0 64 36.0 65 25.5 66 16.0 67 20.5 68 26.0 69 15.0 70 13.5 71 13.0 72 7.5 73 5.5 74 4.0 75 4.0 76 4.5 77 4.5 78 3.5 79 2.5 80 0.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.005 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.22500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35208259166964 55.725 2 11.890352438590245 16.7 3 4.307582769668921 9.075 4 1.957992168031328 5.5 5 1.0323958704165184 3.6249999999999996 6 0.4983980064079744 2.1 7 0.3203987184051264 1.575 8 0.1423994304022784 0.8 9 0.10679957280170879 0.675 >10 0.3915984336062656 4.2250000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 34 0.8500000000000001 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 20 0.5 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 18 0.44999999999999996 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 16 0.4 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 16 0.4 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 13 0.325 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 12 0.3 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 10 0.25 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 10 0.25 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 10 0.25 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 10 0.25 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 9 0.22499999999999998 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 9 0.22499999999999998 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 9 0.22499999999999998 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 8 0.2 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 8 0.2 No Hit GGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGGA 7 0.17500000000000002 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 7 0.17500000000000002 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 7 0.17500000000000002 No Hit GTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCG 7 0.17500000000000002 No Hit GCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCC 7 0.17500000000000002 No Hit GGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAA 6 0.15 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 6 0.15 No Hit GTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGA 6 0.15 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 6 0.15 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 6 0.15 No Hit GGGGTAGAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGC 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit AATGCATTGAGATCGGAAAGAACACCAACGGCGAAAGCACTCTGCTGGGC 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit GTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGA 6 0.15 No Hit TCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTG 6 0.15 No Hit AGAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGG 6 0.15 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 6 0.15 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 5 0.125 No Hit GTTGTGGTTAGGGGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTC 5 0.125 No Hit CGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAACCT 5 0.125 No Hit GTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGG 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit CTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATC 5 0.125 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 5 0.125 No Hit GTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGA 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 5 0.125 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 5 0.125 No Hit GTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTA 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 5 0.125 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 5 0.125 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 5 0.125 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 5 0.125 No Hit GACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCC 5 0.125 No Hit GTTCGGTCCATATCCGGTGTGGGCGTTAGAGCATTGAGAGGACCTTTCCC 5 0.125 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 5 0.125 No Hit GGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCG 5 0.125 No Hit GCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAA 5 0.125 No Hit GAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAGCGCTGCAGCGCTGAA 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.0875 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.1375 0.0 0.0 0.0 0.0 86-87 0.2375 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.5 0.0 0.0 0.0 0.0 94-95 0.625 0.0 0.0 0.0 0.0 96-97 0.7875 0.0 0.0 0.0 0.0 98-99 0.9624999999999999 0.0 0.0 0.0 0.0 100-101 1.1875 0.0 0.0 0.0 0.0 102-103 1.6375 0.0 0.0 0.0 0.0 104-105 1.8624999999999998 0.0 0.0 0.0 0.0 106-107 2.125 0.0 0.0 0.0 0.0 108-109 2.4000000000000004 0.0 0.0 0.0 0.0 110-111 2.75 0.0 0.0 0.0 0.0 112-113 3.275 0.0 0.0 0.0 0.0 114-115 3.9 0.0 0.0 0.0 0.0 116-117 4.3875 0.0 0.0 0.0 0.0 118-119 4.9 0.0 0.0 0.0 0.0 120-121 5.3625 0.0 0.0 0.0 0.0 122-123 5.887499999999999 0.0 0.0 0.0 0.0 124-125 6.6375 0.0 0.0 0.0 0.0 126-127 7.1625 0.0 0.0 0.0 0.0 128-129 7.65 0.0 0.0 0.0 0.0 130-131 8.375 0.0 0.0 0.0 0.0 132-133 9.024999999999999 0.0 0.0 0.0 0.0 134-135 9.8 0.0 0.0 0.0 0.0 136-137 10.7 0.0 0.0 0.0 0.0 138-139 11.4375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATATT 10 0.006830828 145.0 4 ATCTATA 10 0.006830828 145.0 2 TTATTTC 10 0.006830828 145.0 9 TCTATAT 10 0.006830828 145.0 3 TATCTAT 10 0.006830828 145.0 1 CCTTGCA 20 0.00593511 29.0 40-44 CTTGCAA 20 0.00593511 29.0 40-44 >>END_MODULE Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956276 spots for SRR6941611.sra Written 956276 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra Read 956260 spots for SRR6941611.sra Written 956260 spots for SRR6941611.sra SRR ids: ['SRR6941611.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_t1ltfqx_ SRR6941611.sra spots: 19125216 blocks: [[1, 956260], [956261, 1912520], [1912521, 2868780], [2868781, 3825040], [3825041, 4781300], [4781301, 5737560], [5737561, 6693820], [6693821, 7650080], [7650081, 8606340], [8606341, 9562600], [9562601, 10518860], [10518861, 11475120], [11475121, 12431380], [12431381, 13387640], [13387641, 14343900], [14343901, 15300160], [15300161, 16256420], [16256421, 17212680], [17212681, 18168940], [18168941, 19125216]] SRR6941611 file size 6459207 SRR6941611 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941611 SRR6941611_1.fastq SRR6941611_2.fastq Input file: SRR6941611_1.fastq Paired file: SRR6941611_2.fastq trimmed: SRR6941611-trimmed-pair1.fastq, SRR6941611-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 13:08:12 2024 >> started Fri Dec 6 13:08:35 2024 >> done (22.347s) 19125216 read pairs processed; of these: 9893 ( 0.05%) short read pairs filtered out after trimming by size control 7167 ( 0.04%) empty read pairs filtered out after trimming by size control 19108156 (99.91%) read pairs available; of these: 9229663 (48.30%) trimmed read pairs available after processing 9878493 (51.70%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 2 0.00% 20 3 0.00% 21 4 0.00% 22 4 0.00% 23 1 0.00% 24 4 0.00% 25 5 0.00% 26 4 0.00% 27 21 0.00% 28 8 0.00% 29 6 0.00% 30 2 0.00% 31 8 0.00% 32 9 0.00% 33 12 0.00% 34 5 0.00% 35 12 0.00% 36 10 0.00% 37 9 0.00% 38 12 0.00% 39 8 0.00% 40 17 0.00% 41 16 0.00% 42 9 0.00% 43 19 0.00% 44 26 0.00% 45 37 0.00% 46 28 0.00% 47 36 0.00% 48 43 0.00% 49 50 0.00% 50 71 0.00% 51 83 0.00% 52 96 0.00% 53 104 0.00% 54 129 0.00% 55 122 0.00% 56 140 0.00% 57 175 0.00% 58 187 0.00% 59 232 0.00% 60 284 0.00% 61 335 0.00% 62 430 0.00% 63 519 0.00% 64 547 0.00% 65 687 0.00% 66 768 0.00% 67 791 0.00% 68 1014 0.01% 69 1048 0.01% 70 1240 0.01% 71 1370 0.01% 72 1781 0.01% 73 2099 0.01% 74 2120 0.01% 75 2430 0.01% 76 2870 0.02% 77 3119 0.02% 78 3592 0.02% 79 4302 0.02% 80 4786 0.03% 81 5361 0.03% 82 6091 0.03% 83 7334 0.04% 84 7889 0.04% 85 9430 0.05% 86 10012 0.05% 87 10916 0.06% 88 12722 0.07% 89 13228 0.07% 90 14368 0.08% 91 15432 0.08% 92 17442 0.09% 93 18498 0.10% 94 19660 0.10% 95 22479 0.12% 96 22644 0.12% 97 25343 0.13% 98 25556 0.13% 99 27374 0.14% 100 28426 0.15% 101 31647 0.17% 102 32103 0.17% 103 32187 0.17% 104 35139 0.18% 105 35669 0.19% 106 36793 0.19% 107 38624 0.20% 108 43830 0.23% 109 45499 0.24% 110 44779 0.23% 111 46024 0.24% 112 47374 0.25% 113 46955 0.25% 114 51964 0.27% 115 54937 0.29% 116 56129 0.29% 117 54504 0.29% 118 56246 0.29% 119 56007 0.29% 120 60983 0.32% 121 64436 0.34% 122 67161 0.35% 123 70847 0.37% 124 68928 0.36% 125 73910 0.39% 126 73856 0.39% 127 74296 0.39% 128 73305 0.38% 129 78380 0.41% 130 74837 0.39% 131 79399 0.42% 132 83077 0.43% 133 81841 0.43% 134 85570 0.45% 135 86419 0.45% 136 91845 0.48% 137 91058 0.48% 138 98798 0.52% 139 102644 0.54% 140 104212 0.55% 141 118836 0.62% 142 124549 0.65% 143 133466 0.70% 144 150809 0.79% 145 178503 0.93% 146 205970 1.08% 147 259366 1.36% 148 380318 1.99% 149 690754 3.61% 150 4096770 21.44% 151 9878493 51.70% 19108156 reads passed initial QC criterion=sequence-density sequence-density=2.20 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=30 prefix-density=2.17 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=32.39 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=1.1 sequence=TTTGACATCCCCATGCCGCCACACCACAGGGGGGGGACATGGGGGCGTCAAAAAAGGGATCCTATCACTTATCAACTAATTTGTTCCGACCTAGGATAATAAGCTCATGAGCTTGGTCTTACTTCACCCTAAACGAAAGAAGACTTCCATATCCAAGTTTAGCTCAGACGTAGCTGCCTTCTTTTTGGGCGTGAAGCAGTGTCAAACCAAAATACCCAATAAGCATAAGCATTAGCTCTCCCTGAAAAGGAGGTGATCCAGCCGCACCTTCCAGTACGGCTACCTTGTT criterion=sequence-density sequence-density=0.78 sequence-density-rank=1 fanout-score=2.33 fanout-score-rank=30 prefix-density=0.76 prefix-fanout=2.3 sequence=ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=36 fanout-score=50.30 fanout-score-rank=1 prefix-density=1.00 prefix-fanout=1.2 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941611 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 13:09:25 Started mapping on | Dec 06 13:09:26 Finished on | Dec 06 13:11:02 Mapping speed, Million of reads per hour | 716.56 Number of input reads | 19108156 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 8636017 Uniquely mapped reads % | 45.20% Average mapped length | 294.28 Number of splices: Total | 1272881 Number of splices: Annotated (sjdb) | 1139320 Number of splices: GT/AG | 1205979 Number of splices: GC/AG | 15026 Number of splices: AT/AC | 3799 Number of splices: Non-canonical | 48077 Mismatch rate per base, % | 0.17% Deletion rate per base | 0.01% Deletion average length | 1.60 Insertion rate per base | 0.01% Insertion average length | 1.74 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7295810 % of reads mapped to multiple loci | 38.18% Number of reads mapped to too many loci | 406906 % of reads mapped to too many loci | 2.13% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.25% % of reads unmapped: other | 11.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3181606 3181606 3181606 N_multimapping 7295810 7295810 7295810 N_noFeature 5173182 8432925 5254051 N_ambiguous 251967 3909 133746 UnstrandedReadsAssigned:3210868 PositiveStrandReadsAssigned:199183 NegativeStrandReadsAssigned:3248220 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941611 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941611-trimmed-pair1.fastq SRR6941611-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,108,156 reads, 6,592,214 reads pseudoaligned [quant] estimated average fragment length: 210.338 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,075 rounds 52973 SRR6941611.ke.tsv 35125 SRR6941611.se.tsv 88098 total ==> SRR6941611.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 727.18 0 0 PNS24247 1044 834.662 4.08704 0.435754 PNS24249 1928 1718.66 6.73888 0.348932 PNS24246 1044 834.662 4.08704 0.435754 PNS24248 1044 834.662 4.08704 0.435754 PNS24244 1471 1261.66 0 0 PNS24243 293 115.032 0 0 KQK14069 1603 1393.66 503.322 32.139 KQK14071 474 274.06 2.42415 0.787149 ==> SRR6941611.se.tsv <== BRADI_1g14170v3 563 BRADI_1g53295v3 9 BRADI_1g59795v3 13 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 23 BRADI_1g74790v3 9 BRADI_1g09890v3 1 BRADI_1g77505v3 19 BRADI_1g48960v3 0 SRR6941611 completed mapping pipeline successfully