Starting /dee2/code/volunteer_pipeline.sh SRR6941620 current disk space = 1551049736192 free memory = 1598459852 SRR6941620 SRAfilesize f00e1e5b5af8dd9615d5c8a1c500cad5 SRR6941620.sra SRR6941620.sra file validated SRR6941620 is paired end SRR6941620 is conventional basespace SRR6941620 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941620_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69875 34.0 33.0 34.0 32.0 34.0 2 33.06125 34.0 33.0 34.0 32.0 34.0 3 33.15625 34.0 33.0 34.0 32.0 34.0 4 33.3225 34.0 33.0 34.0 33.0 34.0 5 33.32175 34.0 33.0 34.0 33.0 34.0 6 37.26125 38.0 38.0 38.0 36.0 38.0 7 37.5095 38.0 38.0 38.0 37.0 38.0 8 37.61975 38.0 38.0 38.0 38.0 38.0 9 37.66 38.0 38.0 38.0 38.0 38.0 10-14 37.627700000000004 38.0 38.0 38.0 38.0 38.0 15-19 37.6001 38.0 38.0 38.0 38.0 38.0 20-24 37.571200000000005 38.0 38.0 38.0 38.0 38.0 25-29 37.58945 38.0 38.0 38.0 38.0 38.0 30-34 37.50245 38.0 38.0 38.0 38.0 38.0 35-39 37.557 38.0 38.0 38.0 38.0 38.0 40-44 37.5158 38.0 38.0 38.0 37.8 38.0 45-49 37.470600000000005 38.0 38.0 38.0 37.8 38.0 50-54 37.53635 38.0 38.0 38.0 38.0 38.0 55-59 37.4096 38.0 38.0 38.0 37.4 38.0 60-64 37.38575 38.0 38.0 38.0 37.0 38.0 65-69 37.3796 38.0 38.0 38.0 37.0 38.0 70-74 37.29235 38.0 38.0 38.0 37.0 38.0 75-79 37.29035 38.0 38.0 38.0 37.0 38.0 80-84 37.29815 38.0 38.0 38.0 37.0 38.0 85-89 37.232099999999996 38.0 38.0 38.0 36.6 38.0 90-94 37.19875 38.0 38.0 38.0 36.4 38.0 95-99 37.1176 38.0 38.0 38.0 36.0 38.0 100-104 37.065200000000004 38.0 38.0 38.0 36.0 38.0 105-109 37.0113 38.0 38.0 38.0 35.6 38.0 110-114 36.58905 38.0 38.0 38.0 34.8 38.0 115-119 36.58935 38.0 38.0 38.0 34.6 38.0 120-124 36.71445 38.0 38.0 38.0 35.0 38.0 125-129 36.67255 38.0 38.0 38.0 34.8 38.0 130-134 36.468399999999995 38.0 38.0 38.0 34.0 38.0 135-139 36.335699999999996 38.0 38.0 38.0 34.0 38.0 140-144 36.1715 38.0 38.0 38.0 33.2 38.0 145-149 35.81515 38.0 37.2 38.0 32.2 38.0 150-151 32.285125 35.5 32.0 38.0 16.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 0.0 19 2.0 20 2.0 21 0.0 22 3.0 23 2.0 24 3.0 25 9.0 26 8.0 27 8.0 28 15.0 29 26.0 30 26.0 31 37.0 32 38.0 33 65.0 34 95.0 35 191.0 36 381.0 37 3087.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.39483394833948 12.308908803373749 9.040590405904059 45.25566684238271 2 23.45 15.1 34.425 27.025 3 20.125 21.675 25.124999999999996 33.074999999999996 4 26.224999999999998 28.325 22.075 23.375 5 24.325 31.45 23.525 20.7 6 19.15 32.9 26.625 21.325 7 15.775 22.775000000000002 42.0 19.45 8 18.625 20.674999999999997 30.275000000000002 30.425 9 18.175 19.425 33.45 28.95 10-14 21.224999999999998 27.065 24.37 27.339999999999996 15-19 21.945 25.025 26.66 26.369999999999997 20-24 22.105 26.145000000000003 26.419999999999998 25.330000000000002 25-29 22.505 24.785 26.52 26.19 30-34 22.53 26.595000000000002 25.695 25.180000000000003 35-39 21.98 25.895000000000003 26.405 25.72 40-44 22.54 24.959999999999997 26.41 26.090000000000003 45-49 20.412041204120413 26.612661266126615 27.222722272227223 25.752575257525752 50-54 22.05 25.424999999999997 25.814999999999998 26.71 55-59 22.03 25.275 26.3 26.395000000000003 60-64 21.775 25.655 26.32 26.25 65-69 22.075 25.979999999999997 25.64 26.305 70-74 23.705000000000002 25.314999999999998 24.525 26.455000000000002 75-79 22.615 26.0 25.290000000000003 26.095000000000002 80-84 22.665 26.465 25.115 25.755 85-89 22.105 25.974999999999998 26.445 25.474999999999998 90-94 21.985 26.13 24.97 26.915 95-99 22.005 26.205000000000002 24.95 26.840000000000003 100-104 22.021606481944584 26.402920876262883 25.31259377813344 26.2628788636591 105-109 22.439999999999998 25.705 26.029999999999998 25.825 110-114 22.159119465246015 26.541689701965122 26.109463738251996 25.189727094536863 115-119 21.83296526801847 26.124272234491063 25.3864685806063 26.65629391688416 120-124 21.825 26.875 23.535 27.765 125-129 22.525000000000002 26.525 23.974999999999998 26.974999999999998 130-134 22.595000000000002 26.705000000000002 23.56 27.139999999999997 135-139 23.544999999999998 26.674999999999997 24.5 25.28 140-144 23.535 26.575 24.525 25.365 145-149 22.215 26.395000000000003 23.995 27.395000000000003 150-151 21.6 26.737499999999997 23.925 27.737499999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 2.5 23 2.5 24 3.0 25 5.0 26 5.0 27 5.5 28 9.0 29 11.0 30 11.5 31 18.5 32 23.5 33 18.0 34 15.5 35 27.0 36 67.5 37 144.5 38 145.5 39 116.0 40 152.5 41 167.5 42 153.5 43 155.5 44 154.5 45 137.0 46 111.0 47 101.5 48 97.0 49 89.0 50 115.5 51 131.0 52 126.5 53 140.5 54 175.5 55 237.0 56 256.5 57 200.5 58 169.0 59 147.5 60 111.0 61 77.5 62 43.5 63 32.0 64 25.0 65 13.0 66 10.0 67 10.0 68 6.5 69 4.0 70 4.0 71 3.0 72 2.0 73 2.0 74 0.5 75 0.5 76 0.5 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.1499999999999995 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.01 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.03 105-109 0.0 110-114 0.515 115-119 0.38 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.325 #Duplication Level Percentage of deduplicated Percentage of total 1 80.41237113402062 56.55 2 10.913615357269817 15.35 3 4.301457518663349 9.075 4 1.8130110202630643 5.1 5 0.6398862424457874 2.25 6 0.7109847138286527 3.0 7 0.3199431212228937 1.575 8 0.28439388553146105 1.6 9 0.21329541414859582 1.35 >10 0.39104159260575894 4.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 38 0.95 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 22 0.5499999999999999 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 15 0.375 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 14 0.35000000000000003 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 13 0.325 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 12 0.3 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 12 0.3 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 10 0.25 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 10 0.25 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 10 0.25 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 10 0.25 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 9 0.22499999999999998 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 9 0.22499999999999998 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 9 0.22499999999999998 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 9 0.22499999999999998 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 9 0.22499999999999998 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 9 0.22499999999999998 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 8 0.2 No Hit CGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCC 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GGCTGATCATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTAT 8 0.2 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 8 0.2 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 8 0.2 No Hit CTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTT 8 0.2 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 8 0.2 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 7 0.17500000000000002 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 7 0.17500000000000002 No Hit AGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAG 7 0.17500000000000002 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 7 0.17500000000000002 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 7 0.17500000000000002 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 7 0.17500000000000002 No Hit GGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACCGTTT 7 0.17500000000000002 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 7 0.17500000000000002 No Hit CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTC 7 0.17500000000000002 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 6 0.15 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 6 0.15 No Hit ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGG 6 0.15 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 6 0.15 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit CAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCA 6 0.15 No Hit CTTCAAACATGGCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCC 6 0.15 No Hit GGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTG 6 0.15 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 6 0.15 No Hit GCGTTAGCTACAGCACTGCACGGGTCGAGTCGCACAGCACCTAGTATCCA 6 0.15 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 6 0.15 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 6 0.15 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 6 0.15 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 6 0.15 No Hit GTCTATTTCACCGAGCCTCTCTCCGAGACAGTGCCCAGATCGTTACGCCT 6 0.15 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit AAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAG 5 0.125 No Hit CCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTA 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 5 0.125 No Hit ATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAA 5 0.125 No Hit CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAT 5 0.125 No Hit CCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTAGCCC 5 0.125 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 5 0.125 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit CTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATG 5 0.125 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 5 0.125 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 5 0.125 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 5 0.125 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 5 0.125 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0375 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2625 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 1.05 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.675 0.0 0.0 0.0 0.0 100-101 1.9375 0.0 0.0 0.0 0.0 102-103 2.25 0.0 0.0 0.0 0.0 104-105 2.65 0.0 0.0 0.0 0.0 106-107 3.0125 0.0 0.0 0.0 0.0 108-109 3.3625 0.0 0.0 0.0 0.0 110-111 3.75 0.0 0.0 0.0 0.0 112-113 4.175 0.0 0.0 0.0 0.0 114-115 4.5875 0.0 0.0 0.0 0.0 116-117 5.1375 0.0 0.0 0.0 0.0 118-119 5.625 0.0 0.0 0.0 0.0 120-121 6.1 0.0 0.0 0.0 0.0 122-123 6.775 0.0 0.0 0.0 0.0 124-125 7.550000000000001 0.0 0.0 0.0 0.0 126-127 8.1125 0.0 0.0 0.0 0.0 128-129 8.65 0.0 0.0 0.0 0.0 130-131 9.3 0.0 0.0 0.0 0.0 132-133 10.162500000000001 0.0 0.0 0.0 0.0 134-135 10.925 0.0 0.0 0.0 0.0 136-137 11.625 0.0 0.0 0.0 0.0 138-139 12.4375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTCT 10 0.00661466 146.54431 1 CTGCCTT 10 0.006871484 144.71251 8 >>END_MODULE SRR6941620 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941620_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.185 34.0 33.0 34.0 33.0 34.0 2 33.3365 34.0 33.0 34.0 33.0 34.0 3 33.27475 34.0 33.0 34.0 33.0 34.0 4 33.23275 34.0 33.0 34.0 33.0 34.0 5 33.28275 34.0 33.0 34.0 33.0 34.0 6 37.39 38.0 38.0 38.0 38.0 38.0 7 37.43 38.0 38.0 38.0 37.0 38.0 8 37.40475 38.0 38.0 38.0 38.0 38.0 9 37.42975 38.0 38.0 38.0 38.0 38.0 10-14 37.41365 38.0 38.0 38.0 38.0 38.0 15-19 37.4199 38.0 38.0 38.0 38.0 38.0 20-24 37.42795 38.0 38.0 38.0 38.0 38.0 25-29 37.3556 38.0 38.0 38.0 37.2 38.0 30-34 37.364 38.0 38.0 38.0 37.4 38.0 35-39 37.362199999999994 38.0 38.0 38.0 37.2 38.0 40-44 37.34205000000001 38.0 38.0 38.0 37.4 38.0 45-49 37.337300000000006 38.0 38.0 38.0 37.4 38.0 50-54 37.3284 38.0 38.0 38.0 37.2 38.0 55-59 37.285799999999995 38.0 38.0 38.0 37.0 38.0 60-64 37.204899999999995 38.0 38.0 38.0 37.0 38.0 65-69 37.20365 38.0 38.0 38.0 36.8 38.0 70-74 37.1413 38.0 38.0 38.0 36.8 38.0 75-79 37.1331 38.0 38.0 38.0 36.6 38.0 80-84 37.086499999999994 38.0 38.0 38.0 36.4 38.0 85-89 37.06269999999999 38.0 38.0 38.0 36.0 38.0 90-94 37.018249999999995 38.0 38.0 38.0 35.8 38.0 95-99 36.946000000000005 38.0 38.0 38.0 35.8 38.0 100-104 36.74524999999999 38.0 38.0 38.0 35.0 38.0 105-109 36.68079999999999 38.0 38.0 38.0 34.8 38.0 110-114 36.17395 38.0 38.0 38.0 33.4 38.0 115-119 36.133849999999995 38.0 37.8 38.0 33.2 38.0 120-124 36.1426 38.0 37.8 38.0 33.4 38.0 125-129 36.08685 38.0 38.0 38.0 33.2 38.0 130-134 35.9154 38.0 37.6 38.0 32.4 38.0 135-139 35.64015 38.0 36.2 38.0 31.0 38.0 140-144 35.25535000000001 38.0 36.0 38.0 31.0 38.0 145-149 34.1512 38.0 35.2 38.0 26.2 38.0 150-151 29.051125 35.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 0.0 12 0.0 13 3.0 14 2.0 15 0.0 16 0.0 17 1.0 18 1.0 19 0.0 20 0.0 21 5.0 22 6.0 23 5.0 24 5.0 25 12.0 26 17.0 27 17.0 28 28.0 29 28.0 30 30.0 31 54.0 32 59.0 33 81.0 34 126.0 35 217.0 36 462.0 37 2835.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.3 15.2 14.424999999999999 33.074999999999996 2 31.6 17.474999999999998 32.05 18.875 3 22.85 24.099999999999998 30.575000000000003 22.475 4 26.525 32.225 21.875 19.375 5 27.725 32.45 21.9 17.925 6 24.025 33.625 22.625 19.725 7 19.55 18.55 39.15 22.75 8 25.05 22.25 27.250000000000004 25.45 9 25.924999999999997 22.275 28.175 23.625 10-14 27.12 24.82 25.485000000000003 22.575 15-19 26.889999999999997 25.285000000000004 26.44 21.385 20-24 27.055 24.785 26.640000000000004 21.52 25-29 26.86 25.69 25.919999999999998 21.529999999999998 30-34 27.165 25.869999999999997 25.724999999999998 21.240000000000002 35-39 26.6 26.58 25.19 21.63 40-44 26.545 26.595000000000002 25.095 21.765 45-49 25.674999999999997 26.729999999999997 25.105 22.49 50-54 26.919999999999998 25.255 25.785000000000004 22.040000000000003 55-59 26.284999999999997 26.135 25.924999999999997 21.654999999999998 60-64 26.83 25.575 26.515 21.08 65-69 27.87 25.435000000000002 25.430000000000003 21.265 70-74 27.67 25.21 26.195 20.925 75-79 27.125 25.385 25.355 22.134999999999998 80-84 27.0 25.595000000000002 25.835 21.57 85-89 27.155 25.8 24.89 22.155 90-94 27.155 25.88 25.490000000000002 21.475 95-99 27.150000000000002 25.235000000000003 25.6 22.015 100-104 27.165 26.195 26.045 20.595 105-109 27.732773277327734 24.81248124812481 25.917591759175917 21.537153715371538 110-114 25.791289564478227 25.616280814040703 26.466323316165806 22.126106305315265 115-119 28.01 26.029999999999998 24.985 20.974999999999998 120-124 27.43 26.779999999999998 24.13 21.66 125-129 27.560000000000002 26.145000000000003 24.075 22.220000000000002 130-134 27.445000000000004 26.729999999999997 24.395 21.43 135-139 27.500000000000004 25.900000000000002 25.585 21.015 140-144 28.23641182059103 26.21131056552828 24.65623281164058 20.896044802240112 145-149 27.778333500050017 26.54296288886666 25.012503751125337 20.666199859957988 150-151 28.380237648530333 25.465916197623518 25.528455284552848 20.62539086929331 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 2.0 23 3.0 24 5.5 25 7.0 26 6.5 27 6.0 28 7.0 29 9.0 30 13.5 31 14.5 32 15.5 33 23.5 34 35.5 35 47.0 36 61.5 37 91.5 38 118.0 39 124.0 40 148.0 41 167.5 42 158.0 43 154.5 44 160.5 45 145.0 46 118.0 47 116.5 48 105.5 49 94.5 50 96.0 51 103.0 52 113.0 53 151.5 54 225.5 55 259.0 56 211.0 57 159.5 58 150.0 59 136.5 60 112.0 61 89.0 62 72.5 63 44.0 64 22.5 65 16.0 66 8.5 67 13.0 68 21.0 69 15.0 70 6.0 71 4.5 72 4.0 73 2.5 74 2.0 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.01 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.03 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2403676210675 56.75 2 11.417462000706964 16.150000000000002 3 3.9589961117002477 8.4 4 1.7674089784376106 5.0 5 0.9190526687875575 3.25 6 0.5655708731000354 2.4 7 0.3534817956875221 1.7500000000000002 8 0.2827854365500177 1.6 9 0.14139271827500885 0.8999999999999999 >10 0.3534817956875221 3.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 31 0.775 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 22 0.5499999999999999 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 17 0.42500000000000004 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 14 0.35000000000000003 No Hit CCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCG 13 0.325 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 12 0.3 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 12 0.3 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 11 0.27499999999999997 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 10 0.25 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 10 0.25 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 9 0.22499999999999998 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 9 0.22499999999999998 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 8 0.2 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 8 0.2 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 8 0.2 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 8 0.2 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 8 0.2 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 8 0.2 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 8 0.2 No Hit GTCAAATCCCAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTG 8 0.2 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 7 0.17500000000000002 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 7 0.17500000000000002 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 6 0.15 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 6 0.15 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 6 0.15 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 6 0.15 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 6 0.15 No Hit CTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTT 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit TGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTA 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 6 0.15 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 6 0.15 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 5 0.125 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 5 0.125 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 5 0.125 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 5 0.125 No Hit TGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTT 5 0.125 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 5 0.125 No Hit CAGGGCTCAACCCTGGACAGGCGGTGGAAACTACCAAGCTGGAGTACGGT 5 0.125 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 5 0.125 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAG 5 0.125 No Hit CGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCAT 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit GGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACG 5 0.125 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 5 0.125 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 5 0.125 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 5 0.125 No Hit GGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAG 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0375 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2625 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 0.8125 0.0 0.0 0.0 0.0 94-95 1.05 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.65 0.0 0.0 0.0 0.0 100-101 1.9125 0.0 0.0 0.0 0.0 102-103 2.225 0.0 0.0 0.0 0.0 104-105 2.625 0.0 0.0 0.0 0.0 106-107 3.0125 0.0 0.0 0.0 0.0 108-109 3.375 0.0 0.0 0.0 0.0 110-111 3.775 0.0 0.0 0.0 0.0 112-113 4.2125 0.0 0.0 0.0 0.0 114-115 4.65 0.0 0.0 0.0 0.0 116-117 5.2125 0.0 0.0 0.0 0.0 118-119 5.699999999999999 0.0 0.0 0.0 0.0 120-121 6.1875 0.0 0.0 0.0 0.0 122-123 6.875 0.0 0.0 0.0 0.0 124-125 7.6625 0.0 0.0 0.0 0.0 126-127 8.2125 0.0 0.0 0.0 0.0 128-129 8.75 0.0 0.0 0.0 0.0 130-131 9.425 0.0 0.0 0.0 0.0 132-133 10.287500000000001 0.0 0.0 0.0 0.0 134-135 11.0375 0.0 0.0 0.0 0.0 136-137 11.6875 0.0 0.0 0.0 0.0 138-139 12.5125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGGAG 10 0.006830828 145.0 1 GGCTCAA 10 0.006830828 145.0 5 CTTGCGG 15 1.1411342E-4 145.0 1 TTGCGGT 15 1.1411342E-4 145.0 2 GGTGGAT 15 1.1411342E-4 145.0 6 GTGGATA 15 1.1411342E-4 145.0 7 TGCGGTG 15 1.1411342E-4 145.0 3 TATGGTC 10 0.006830828 145.0 9 GCGGTGG 20 3.5877043E-4 108.75 4 GGATACC 20 3.5877043E-4 108.75 9 AGAGACA 20 3.5877043E-4 108.75 145 CGGTGGA 30 0.0017973486 72.5 5 TGGATAC 35 0.0033124194 62.14286 8 >>END_MODULE Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049617 spots for SRR6941620.sra Written 1049617 spots for SRR6941620.sra Read 1049628 spots for SRR6941620.sra Written 1049628 spots for SRR6941620.sra SRR ids: ['SRR6941620.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hbugwj5r SRR6941620.sra spots: 20992351 blocks: [[1, 1049617], [1049618, 2099234], [2099235, 3148851], [3148852, 4198468], [4198469, 5248085], [5248086, 6297702], [6297703, 7347319], [7347320, 8396936], [8396937, 9446553], [9446554, 10496170], [10496171, 11545787], [11545788, 12595404], [12595405, 13645021], [13645022, 14694638], [14694639, 15744255], [15744256, 16793872], [16793873, 17843489], [17843490, 18893106], [18893107, 19942723], [19942724, 20992351]] SRR6941620 file size 7091918 SRR6941620 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941620 SRR6941620_1.fastq SRR6941620_2.fastq Input file: SRR6941620_1.fastq Paired file: SRR6941620_2.fastq trimmed: SRR6941620-trimmed-pair1.fastq, SRR6941620-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 13:28:59 2024 >> started Fri Dec 6 13:29:22 2024 >> done (22.648s) 20992351 read pairs processed; of these: 9421 ( 0.04%) short read pairs filtered out after trimming by size control 10959 ( 0.05%) empty read pairs filtered out after trimming by size control 20971971 (99.90%) read pairs available; of these: 10165206 (48.47%) trimmed read pairs available after processing 10806765 (51.53%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 1 0.00% 20 4 0.00% 21 4 0.00% 22 3 0.00% 23 4 0.00% 24 3 0.00% 25 3 0.00% 26 14 0.00% 27 13 0.00% 28 8 0.00% 29 5 0.00% 30 10 0.00% 31 13 0.00% 32 7 0.00% 33 11 0.00% 34 15 0.00% 35 24 0.00% 36 31 0.00% 37 21 0.00% 38 29 0.00% 39 27 0.00% 40 31 0.00% 41 31 0.00% 42 51 0.00% 43 56 0.00% 44 59 0.00% 45 61 0.00% 46 71 0.00% 47 87 0.00% 48 88 0.00% 49 130 0.00% 50 135 0.00% 51 164 0.00% 52 229 0.00% 53 208 0.00% 54 268 0.00% 55 293 0.00% 56 313 0.00% 57 377 0.00% 58 421 0.00% 59 531 0.00% 60 558 0.00% 61 735 0.00% 62 883 0.00% 63 975 0.00% 64 1112 0.01% 65 1305 0.01% 66 1395 0.01% 67 1610 0.01% 68 1818 0.01% 69 1952 0.01% 70 2319 0.01% 71 2577 0.01% 72 3187 0.02% 73 3595 0.02% 74 3815 0.02% 75 4510 0.02% 76 4950 0.02% 77 5509 0.03% 78 6087 0.03% 79 6975 0.03% 80 8063 0.04% 81 8628 0.04% 82 9806 0.05% 83 10898 0.05% 84 11890 0.06% 85 14158 0.07% 86 14651 0.07% 87 15795 0.08% 88 17897 0.09% 89 18785 0.09% 90 20130 0.10% 91 21131 0.10% 92 24061 0.11% 93 25141 0.12% 94 25801 0.12% 95 29296 0.14% 96 28749 0.14% 97 31314 0.15% 98 32862 0.16% 99 34909 0.17% 100 35382 0.17% 101 39019 0.19% 102 38546 0.18% 103 38682 0.18% 104 41267 0.20% 105 42103 0.20% 106 44070 0.21% 107 46333 0.22% 108 49026 0.23% 109 49872 0.24% 110 49468 0.24% 111 50862 0.24% 112 51868 0.25% 113 52478 0.25% 114 56095 0.27% 115 60151 0.29% 116 62083 0.30% 117 59622 0.28% 118 61056 0.29% 119 60311 0.29% 120 64341 0.31% 121 66770 0.32% 122 69658 0.33% 123 72650 0.35% 124 72268 0.34% 125 78797 0.38% 126 74699 0.36% 127 77985 0.37% 128 77986 0.37% 129 80482 0.38% 130 78318 0.37% 131 81980 0.39% 132 84661 0.40% 133 85107 0.41% 134 88540 0.42% 135 89542 0.43% 136 94005 0.45% 137 95921 0.46% 138 102563 0.49% 139 107242 0.51% 140 109858 0.52% 141 125749 0.60% 142 129341 0.62% 143 142365 0.68% 144 158026 0.75% 145 188334 0.90% 146 219221 1.05% 147 275415 1.31% 148 410625 1.96% 149 752266 3.59% 150 4558508 21.74% 151 10806765 51.53% 20971971 reads passed initial QC criterion=sequence-density sequence-density=1.12 sequence-density-rank=1 fanout-score=2.46 fanout-score-rank=24 prefix-density=1.15 prefix-fanout=2.4 sequence=GTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGTTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTGCGACCCCCTTTTGTGAGGGGGCACCCCTTCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=302.85 fanout-score-rank=1 prefix-density=2.26 prefix-fanout=1.0 sequence=GTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCG criterion=sequence-density sequence-density=0.78 sequence-density-rank=1 fanout-score=2.31 fanout-score-rank=29 prefix-density=0.77 prefix-fanout=2.3 sequence=ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTCGGATTCTAACCTTGTGTCAGACCCGCGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=109.36 fanout-score-rank=1 prefix-density=0.99 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941620 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 13:29:54 Started mapping on | Dec 06 13:29:54 Finished on | Dec 06 13:32:09 Mapping speed, Million of reads per hour | 559.25 Number of input reads | 20971971 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 10143678 Uniquely mapped reads % | 48.37% Average mapped length | 294.11 Number of splices: Total | 1403926 Number of splices: Annotated (sjdb) | 1261926 Number of splices: GT/AG | 1335609 Number of splices: GC/AG | 16362 Number of splices: AT/AC | 4989 Number of splices: Non-canonical | 46966 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.02% Deletion average length | 1.94 Insertion rate per base | 0.01% Insertion average length | 2.30 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7810334 % of reads mapped to multiple loci | 37.24% Number of reads mapped to too many loci | 398761 % of reads mapped to too many loci | 1.90% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.69% % of reads unmapped: other | 9.80% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3022947 3022947 3022947 N_multimapping 7810334 7810334 7810334 N_noFeature 5949802 9901394 6047656 N_ambiguous 278558 4258 137213 UnstrandedReadsAssigned:3915318 PositiveStrandReadsAssigned:238026 NegativeStrandReadsAssigned:3958809 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941620 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941620-trimmed-pair1.fastq SRR6941620-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,971,971 reads, 7,467,199 reads pseudoaligned [quant] estimated average fragment length: 209.924 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,240 rounds 52973 SRR6941620.ke.tsv 35125 SRR6941620.se.tsv 88098 total ==> SRR6941620.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 727.482 0 0 PNS24247 1044 835.076 4.97581 0.483182 PNS24249 1928 1719.08 0 0 PNS24246 1044 835.076 4.97581 0.483182 PNS24248 1044 835.076 4.97581 0.483182 PNS24244 1471 1262.08 16.0726 1.0327 PNS24243 293 115.132 0 0 KQK14069 1603 1394.08 136.913 7.96401 KQK14071 474 274.258 0 0 ==> SRR6941620.se.tsv <== BRADI_1g14170v3 196 BRADI_1g53295v3 20 BRADI_1g59795v3 5 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 29 BRADI_1g74790v3 14 BRADI_1g09890v3 0 BRADI_1g77505v3 13 BRADI_1g48960v3 0 SRR6941620 completed mapping pipeline successfully