Starting /dee2/code/volunteer_pipeline.sh SRR6941621 current disk space = 1551121166336 free memory = 1351425316 SRR6941621 SRAfilesize 038184831652ac2d9685528c4be06400 SRR6941621.sra SRR6941621.sra file validated SRR6941621 is paired end SRR6941621 is conventional basespace SRR6941621 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941621_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48 34.0 33.0 34.0 31.0 34.0 2 32.88625 34.0 33.0 34.0 31.0 34.0 3 33.08975 34.0 33.0 34.0 32.0 34.0 4 33.3285 34.0 33.0 34.0 32.0 34.0 5 33.38375 34.0 33.0 34.0 33.0 34.0 6 37.21775 38.0 38.0 38.0 36.0 38.0 7 37.50125 38.0 38.0 38.0 37.0 38.0 8 37.5855 38.0 38.0 38.0 38.0 38.0 9 37.54675 38.0 38.0 38.0 38.0 38.0 10-14 37.596199999999996 38.0 38.0 38.0 38.0 38.0 15-19 37.62795 38.0 38.0 38.0 38.0 38.0 20-24 37.58815 38.0 38.0 38.0 38.0 38.0 25-29 37.55795 38.0 38.0 38.0 38.0 38.0 30-34 37.50045 38.0 38.0 38.0 37.8 38.0 35-39 37.5509 38.0 38.0 38.0 38.0 38.0 40-44 37.530049999999996 38.0 38.0 38.0 37.8 38.0 45-49 37.469800000000006 38.0 38.0 38.0 37.4 38.0 50-54 37.558550000000004 38.0 38.0 38.0 38.0 38.0 55-59 37.520950000000006 38.0 38.0 38.0 38.0 38.0 60-64 37.4559 38.0 38.0 38.0 37.6 38.0 65-69 37.40205 38.0 38.0 38.0 37.0 38.0 70-74 37.2579 38.0 38.0 38.0 37.0 38.0 75-79 37.33715 38.0 38.0 38.0 37.0 38.0 80-84 37.3085 38.0 38.0 38.0 37.0 38.0 85-89 37.2045 38.0 38.0 38.0 37.0 38.0 90-94 37.212300000000006 38.0 38.0 38.0 36.6 38.0 95-99 37.1708 38.0 38.0 38.0 36.0 38.0 100-104 37.16225 38.0 38.0 38.0 36.0 38.0 105-109 36.99145 38.0 38.0 38.0 35.6 38.0 110-114 36.602549999999994 38.0 38.0 38.0 34.8 38.0 115-119 36.60385 38.0 38.0 38.0 34.6 38.0 120-124 36.800349999999995 38.0 38.0 38.0 35.0 38.0 125-129 36.655150000000006 38.0 38.0 38.0 35.0 38.0 130-134 36.479850000000006 38.0 38.0 38.0 34.0 38.0 135-139 36.316649999999996 38.0 38.0 38.0 33.8 38.0 140-144 36.1874 38.0 38.0 38.0 33.2 38.0 145-149 35.92 38.0 38.0 38.0 33.0 38.0 150-151 32.34425 36.0 32.5 38.0 17.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 1.0 16 0.0 17 1.0 18 1.0 19 1.0 20 1.0 21 0.0 22 5.0 23 1.0 24 5.0 25 3.0 26 10.0 27 9.0 28 19.0 29 13.0 30 23.0 31 44.0 32 34.0 33 63.0 34 105.0 35 173.0 36 381.0 37 3106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.69250985545335 10.170827858081472 8.567674113009199 47.56898817345598 2 22.05551387846962 14.253563390847713 35.20880220055014 28.482120530132534 3 19.3 18.975 24.6 37.125 4 25.275 27.35 22.6 24.775 5 24.3 29.975 23.599999999999998 22.125 6 20.8 32.15 23.95 23.1 7 16.025 20.974999999999998 40.699999999999996 22.3 8 20.075000000000003 19.85 30.4 29.675 9 19.075 19.525000000000002 32.300000000000004 29.099999999999998 10-14 22.395 25.669999999999998 24.825 27.11 15-19 22.355 23.54 26.58 27.525 20-24 22.925 24.55 25.814999999999998 26.71 25-29 23.015 23.965 26.005 27.015 30-34 22.93 24.39 25.91 26.77 35-39 22.365 24.305 26.340000000000003 26.99 40-44 22.814999999999998 23.64 26.415 27.13 45-49 21.241062053102656 23.971198559928 27.281364068203413 27.50637531876594 50-54 21.95 23.7 25.935000000000002 28.415000000000003 55-59 21.88 24.335 26.13 27.655 60-64 22.355 24.085 27.07 26.490000000000002 65-69 22.975 24.365000000000002 24.665 27.994999999999997 70-74 23.805 24.41 24.89 26.895000000000003 75-79 22.53 24.455 26.1 26.915 80-84 23.3 24.395 25.27 27.034999999999997 85-89 21.765 24.43 26.1 27.705000000000002 90-94 22.939999999999998 25.1 24.295 27.665 95-99 22.415 25.25 25.014999999999997 27.32 100-104 22.680670167541887 25.166291572893222 25.166291572893222 26.98674668667167 105-109 23.135 24.34 25.44 27.084999999999997 110-114 22.62388913993071 24.617161219059096 25.601245167444898 27.157704473565296 115-119 22.35707121364092 25.305917753259777 25.020060180541627 27.316950852557675 120-124 22.35 25.779999999999998 23.565 28.305000000000003 125-129 22.650000000000002 24.675 23.835 28.84 130-134 23.48 25.305 23.825 27.389999999999997 135-139 23.89 24.87 24.92 26.32 140-144 23.79 25.380000000000003 24.345 26.484999999999996 145-149 22.84 24.725 24.33 28.105000000000004 150-151 22.3375 26.087500000000002 24.087500000000002 27.487499999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.5 22 1.0 23 0.5 24 1.5 25 4.0 26 4.0 27 3.5 28 6.5 29 8.5 30 9.5 31 10.5 32 16.0 33 17.5 34 17.0 35 26.0 36 54.5 37 103.5 38 102.0 39 86.0 40 116.5 41 122.5 42 110.0 43 110.0 44 107.5 45 123.0 46 126.5 47 95.0 48 89.5 49 109.5 50 136.0 51 152.5 52 157.5 53 181.0 54 220.0 55 281.5 56 302.5 57 232.0 58 183.5 59 161.0 60 122.5 61 84.5 62 51.0 63 40.0 64 31.0 65 21.0 66 13.0 67 7.0 68 4.0 69 4.0 70 6.5 71 6.5 72 4.5 73 2.0 74 0.0 75 2.5 76 3.5 77 1.0 78 0.5 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.875 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.005 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.025 105-109 0.0 110-114 0.415 115-119 0.3 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.75 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21146953405018 55.25 2 11.362007168458781 15.85 3 4.623655913978494 9.675 4 2.3655913978494625 6.6000000000000005 5 0.9318996415770611 3.25 6 0.5734767025089605 2.4 7 0.17921146953405018 0.8750000000000001 8 0.17921146953405018 1.0 9 0.14336917562724014 0.8999999999999999 >10 0.43010752688172044 4.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 36 0.8999999999999999 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 15 0.375 No Hit GTTTACGGCTAGGACTACTGGGGTCTCTAATCCCATTTGCTCCCCTAGCT 15 0.375 No Hit CTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGT 13 0.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 13 0.325 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 12 0.3 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 12 0.3 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 11 0.27499999999999997 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 11 0.27499999999999997 No Hit CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGC 10 0.25 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 10 0.25 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 10 0.25 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 9 0.22499999999999998 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 9 0.22499999999999998 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 9 0.22499999999999998 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 9 0.22499999999999998 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 8 0.2 No Hit GGCATGATGACTTGGCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCG 8 0.2 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit CCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCA 8 0.2 No Hit CCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTA 7 0.17500000000000002 No Hit CCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAACCTTGGGTTTT 7 0.17500000000000002 No Hit CCTGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAACTGAGGACGGGT 7 0.17500000000000002 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 7 0.17500000000000002 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 7 0.17500000000000002 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 6 0.15 No Hit GGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACC 6 0.15 No Hit CGTTGAGCGACGGCCCTTCCACTCGGCACCGTCGGATCACTAAGGCCGAC 6 0.15 No Hit CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA 6 0.15 No Hit CGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGAC 6 0.15 No Hit CTCTCCTTCCTCCGGCTTAACACCGGCGGTCTGTTCAGGGTTCCAAACTC 6 0.15 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 6 0.15 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 6 0.15 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 6 0.15 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 6 0.15 No Hit CATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTC 6 0.15 No Hit CCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCAT 6 0.15 No Hit GCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGT 6 0.15 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 6 0.15 No Hit CTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGG 5 0.125 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit CACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAA 5 0.125 No Hit CCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCAC 5 0.125 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 5 0.125 No Hit CTCCTTTATCACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGC 5 0.125 No Hit GGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTGGCACAGAGTTAG 5 0.125 No Hit CCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCT 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATA 5 0.125 No Hit CTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCT 5 0.125 No Hit GCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCC 5 0.125 No Hit GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC 5 0.125 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 5 0.125 No Hit CTTGGGCGGATTTCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAACC 5 0.125 No Hit GTCCGTCTGGCCCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAG 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit CATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAAT 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 5 0.125 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit CCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGA 5 0.125 No Hit CATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGA 5 0.125 No Hit GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.5249999999999999 0.0 0.0 0.0 0.0 88-89 0.7 0.0 0.0 0.0 0.0 90-91 0.7875000000000001 0.0 0.0 0.0 0.0 92-93 0.925 0.0 0.0 0.0 0.0 94-95 1.1124999999999998 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.6125 0.0 0.0 0.0 0.0 100-101 2.0125 0.0 0.0 0.0 0.0 102-103 2.4375 0.0 0.0 0.0 0.0 104-105 2.6125 0.0 0.0 0.0 0.0 106-107 3.025 0.0 0.0 0.0 0.0 108-109 3.3125 0.0 0.0 0.0 0.0 110-111 3.8625000000000003 0.0 0.0 0.0 0.0 112-113 4.362500000000001 0.0 0.0 0.0 0.0 114-115 4.762499999999999 0.0 0.0 0.0 0.0 116-117 5.3625 0.0 0.0 0.0 0.0 118-119 5.875 0.0 0.0 0.0 0.0 120-121 6.387499999999999 0.0 0.0 0.0 0.0 122-123 6.975 0.0 0.0 0.0 0.0 124-125 7.6 0.0 0.0 0.0 0.0 126-127 8.1375 0.0 0.0 0.0 0.0 128-129 8.6875 0.0 0.0 0.0 0.0 130-131 9.2 0.0 0.0 0.0 0.0 132-133 9.7625 0.0 0.0 0.0 0.0 134-135 10.3125 0.0 0.0 0.0 0.0 136-137 10.9875 0.0 0.0 0.0 0.0 138-139 11.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCACG 10 0.0059115817 152.07895 1 GCCACGC 10 0.006905315 144.475 2 TGCACGT 20 0.0060412474 28.895 75-79 >>END_MODULE SRR6941621 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR6941621_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.13225 34.0 33.0 34.0 33.0 34.0 2 33.296 34.0 33.0 34.0 33.0 34.0 3 33.2735 34.0 33.0 34.0 33.0 34.0 4 33.21375 34.0 33.0 34.0 33.0 34.0 5 33.299 34.0 33.0 34.0 33.0 34.0 6 37.393 38.0 38.0 38.0 37.0 38.0 7 37.45 38.0 38.0 38.0 38.0 38.0 8 37.44475 38.0 38.0 38.0 38.0 38.0 9 37.42525 38.0 38.0 38.0 38.0 38.0 10-14 37.37245 38.0 38.0 38.0 37.6 38.0 15-19 37.41705 38.0 38.0 38.0 38.0 38.0 20-24 37.3803 38.0 38.0 38.0 38.0 38.0 25-29 37.3358 38.0 38.0 38.0 37.6 38.0 30-34 37.37669999999999 38.0 38.0 38.0 37.8 38.0 35-39 37.3697 38.0 38.0 38.0 38.0 38.0 40-44 37.3406 38.0 38.0 38.0 37.2 38.0 45-49 37.33305 38.0 38.0 38.0 37.8 38.0 50-54 37.3135 38.0 38.0 38.0 37.2 38.0 55-59 37.24294999999999 38.0 38.0 38.0 37.0 38.0 60-64 37.13539999999999 38.0 38.0 38.0 37.0 38.0 65-69 37.1771 38.0 38.0 38.0 37.0 38.0 70-74 37.134299999999996 38.0 38.0 38.0 36.8 38.0 75-79 37.14015 38.0 38.0 38.0 36.4 38.0 80-84 37.11255 38.0 38.0 38.0 36.6 38.0 85-89 37.03144999999999 38.0 38.0 38.0 36.0 38.0 90-94 37.00865 38.0 38.0 38.0 36.0 38.0 95-99 36.91265 38.0 38.0 38.0 35.4 38.0 100-104 36.7401 38.0 38.0 38.0 35.0 38.0 105-109 36.5758 38.0 38.0 38.0 34.8 38.0 110-114 36.1436 38.0 38.0 38.0 33.6 38.0 115-119 36.06825 38.0 38.0 38.0 33.4 38.0 120-124 36.05655 38.0 38.0 38.0 33.4 38.0 125-129 36.10535 38.0 38.0 38.0 33.6 38.0 130-134 35.98825000000001 38.0 38.0 38.0 32.6 38.0 135-139 35.79765 38.0 37.8 38.0 31.4 38.0 140-144 35.5545 38.0 36.6 38.0 31.2 38.0 145-149 34.52905 38.0 35.6 38.0 27.8 38.0 150-151 29.842750000000002 35.5 27.5 38.0 14.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 1.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.0 14 2.0 15 1.0 16 2.0 17 1.0 18 2.0 19 3.0 20 4.0 21 3.0 22 6.0 23 7.0 24 4.0 25 15.0 26 14.0 27 18.0 28 25.0 29 15.0 30 34.0 31 42.0 32 56.0 33 68.0 34 132.0 35 167.0 36 467.0 37 2902.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.45 14.875 11.825 32.85 2 31.75 18.325 31.075000000000003 18.85 3 24.4 22.225 30.0 23.375 4 28.449999999999996 28.625 23.075000000000003 19.85 5 29.025000000000002 30.099999999999998 22.15 18.725 6 24.224999999999998 34.35 20.599999999999998 20.825 7 22.15 19.650000000000002 35.375 22.825 8 25.3 21.825 26.85 26.025 9 28.799999999999997 21.575 26.825 22.8 10-14 28.165000000000003 25.21 23.705000000000002 22.919999999999998 15-19 27.93 25.835 24.36 21.875 20-24 27.615000000000002 25.314999999999998 24.990000000000002 22.08 25-29 28.305000000000003 26.040000000000003 23.715 21.94 30-34 27.76 26.695 24.125 21.42 35-39 28.065 26.455000000000002 23.825 21.654999999999998 40-44 28.205000000000002 25.505 24.349999999999998 21.94 45-49 26.790000000000003 26.86 23.775 22.575 50-54 28.27 25.295 24.415 22.02 55-59 26.700000000000003 26.450000000000003 24.85 22.0 60-64 27.87 24.015 25.345000000000002 22.770000000000003 65-69 29.07 25.324999999999996 23.630000000000003 21.975 70-74 28.22 24.635 25.035 22.11 75-79 28.23 24.709999999999997 24.104999999999997 22.955000000000002 80-84 27.415 25.635 24.985 21.965 85-89 28.01 25.080000000000002 24.224999999999998 22.685 90-94 28.37 25.88 23.544999999999998 22.205 95-99 27.97 24.93 24.169999999999998 22.93 100-104 28.044999999999998 26.245 24.65 21.060000000000002 105-109 29.04145207260363 24.64623231161558 25.09125456272814 21.221061053052654 110-114 27.794999999999998 25.05 25.1 22.055 115-119 28.349999999999998 25.955000000000002 23.9 21.795 120-124 28.62 26.090000000000003 22.875 22.415 125-129 28.285 26.445 22.78 22.49 130-134 28.299999999999997 26.615 23.155 21.93 135-139 28.015 26.174999999999997 23.865 21.945 140-144 29.24 25.75 23.785 21.224999999999998 145-149 28.432843284328435 26.1976197619762 23.647364736473648 21.722172217221722 150-151 28.99112389048631 25.053131641455185 23.85298162270284 22.10276284535567 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 0.5 22 0.0 23 1.0 24 2.0 25 3.0 26 5.5 27 5.5 28 7.5 29 14.0 30 14.0 31 9.5 32 11.0 33 19.0 34 32.5 35 41.5 36 43.0 37 74.0 38 99.5 39 106.5 40 117.0 41 110.0 42 105.5 43 113.5 44 130.0 45 126.0 46 113.0 47 114.5 48 97.5 49 88.0 50 112.5 51 124.0 52 115.0 53 154.0 54 238.5 55 312.0 56 279.5 57 181.5 58 150.0 59 162.0 60 140.0 61 106.5 62 92.5 63 56.0 64 30.0 65 23.5 66 16.5 67 24.5 68 26.5 69 14.0 70 8.0 71 4.0 72 3.0 73 5.0 74 4.5 75 2.5 76 2.5 77 2.0 78 1.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.005 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.01 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.825 #Duplication Level Percentage of deduplicated Percentage of total 1 78.98317221625493 55.15 2 12.567132116004295 17.549999999999997 3 4.22484783387039 8.85 4 1.8259935553168638 5.1 5 0.8592910848549946 3.0 6 0.5728607232366631 2.4 7 0.35803795202291444 1.7500000000000002 8 0.2506265664160401 1.4000000000000001 9 0.0 0.0 >10 0.35803795202291444 4.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 36 0.8999999999999999 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 34 0.8500000000000001 No Hit CCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAAC 21 0.525 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 19 0.475 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 19 0.475 No Hit GTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGT 15 0.375 No Hit CTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCC 14 0.35000000000000003 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 12 0.3 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 12 0.3 No Hit GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 10 0.25 No Hit CAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGG 8 0.2 No Hit CACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGG 8 0.2 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 8 0.2 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 8 0.2 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 8 0.2 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 8 0.2 No Hit CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGT 8 0.2 No Hit GAACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGT 7 0.17500000000000002 No Hit GAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGTT 7 0.17500000000000002 No Hit GGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGGTGCT 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 7 0.17500000000000002 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 7 0.17500000000000002 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 7 0.17500000000000002 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 7 0.17500000000000002 No Hit CTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGC 6 0.15 No Hit CTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCG 6 0.15 No Hit GCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAG 6 0.15 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 6 0.15 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 6 0.15 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit GACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCG 6 0.15 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit ACCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGC 6 0.15 No Hit AGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAG 6 0.15 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 6 0.15 No Hit GGGGAATTTTCCGCAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGA 6 0.15 No Hit GCTTAACACATGCAAGTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGG 6 0.15 No Hit CTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGG 6 0.15 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGT 5 0.125 No Hit GCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTCGTCAAC 5 0.125 No Hit GGATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGG 5 0.125 No Hit TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGT 5 0.125 No Hit CTGAGGAATAAGCATCGGCTAACTCTGTGCCAGCAGCCGCGGTAAGACAG 5 0.125 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 5 0.125 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 5 0.125 No Hit GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGT 5 0.125 No Hit GCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGC 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit CTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGT 5 0.125 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 5 0.125 No Hit TGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCA 5 0.125 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 5 0.125 No Hit CAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 5 0.125 No Hit CGGCGAGCGAAATGGGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAG 5 0.125 No Hit GCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGG 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit GCATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAG 5 0.125 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 5 0.125 No Hit GTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.5249999999999999 0.0 0.0 0.0 0.0 88-89 0.7 0.0 0.0 0.0 0.0 90-91 0.7875000000000001 0.0 0.0 0.0 0.0 92-93 0.925 0.0 0.0 0.0 0.0 94-95 1.125 0.0 0.0 0.0 0.0 96-97 1.35 0.0 0.0 0.0 0.0 98-99 1.6375 0.0 0.0 0.0 0.0 100-101 2.0625 0.0 0.0 0.0 0.0 102-103 2.4875 0.0 0.0 0.0 0.0 104-105 2.6625 0.0 0.0 0.0 0.0 106-107 3.075 0.0 0.0 0.0 0.0 108-109 3.3625 0.0 0.0 0.0 0.0 110-111 3.9125 0.0 0.0 0.0 0.0 112-113 4.4625 0.0 0.0 0.0 0.0 114-115 4.887499999999999 0.0 0.0 0.0 0.0 116-117 5.4875 0.0 0.0 0.0 0.0 118-119 6.0 0.0 0.0 0.0 0.0 120-121 6.5375 0.0 0.0 0.0 0.0 122-123 7.15 0.0 0.0 0.0 0.0 124-125 7.775 0.0 0.0 0.0 0.0 126-127 8.3125 0.0 0.0 0.0 0.0 128-129 8.85 0.0 0.0 0.0 0.0 130-131 9.3625 0.0 0.0 0.0 0.0 132-133 9.912500000000001 0.0 0.0 0.0 0.0 134-135 10.4875 0.0 0.0 0.0 0.0 136-137 11.1375 0.0 0.0 0.0 0.0 138-139 11.725000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCCAG 10 0.0068502324 144.8625 3 CCAGACA 10 0.0068502324 144.8625 6 TCCAGAC 10 0.0068502324 144.8625 5 GACATAG 10 0.0068502324 144.8625 9 GTCCAGA 10 0.0068502324 144.8625 4 AGGTCCA 10 0.0068502324 144.8625 2 CAGACAT 10 0.0068502324 144.8625 7 CAGGTCC 10 0.0068502324 144.8625 1 AGACATA 10 0.0068502324 144.8625 8 >>END_MODULE Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972585 spots for SRR6941621.sra Written 972585 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra Read 972566 spots for SRR6941621.sra Written 972566 spots for SRR6941621.sra SRR ids: ['SRR6941621.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jti5rs1o SRR6941621.sra spots: 19451339 blocks: [[1, 972566], [972567, 1945132], [1945133, 2917698], [2917699, 3890264], [3890265, 4862830], [4862831, 5835396], [5835397, 6807962], [6807963, 7780528], [7780529, 8753094], [8753095, 9725660], [9725661, 10698226], [10698227, 11670792], [11670793, 12643358], [12643359, 13615924], [13615925, 14588490], [14588491, 15561056], [15561057, 16533622], [16533623, 17506188], [17506189, 18478754], [18478755, 19451339]] SRR6941621 file size 6569720 SRR6941621 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR6941621 SRR6941621_1.fastq SRR6941621_2.fastq Input file: SRR6941621_1.fastq Paired file: SRR6941621_2.fastq trimmed: SRR6941621-trimmed-pair1.fastq, SRR6941621-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 13:33:19 2024 >> started Fri Dec 6 13:33:42 2024 >> done (22.590s) 19451339 read pairs processed; of these: 6807 ( 0.03%) short read pairs filtered out after trimming by size control 8903 ( 0.05%) empty read pairs filtered out after trimming by size control 19435629 (99.92%) read pairs available; of these: 9167021 (47.17%) trimmed read pairs available after processing 10268608 (52.83%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 4 0.00% 20 6 0.00% 21 3 0.00% 22 4 0.00% 23 6 0.00% 24 6 0.00% 25 8 0.00% 26 4 0.00% 27 11 0.00% 28 12 0.00% 29 15 0.00% 30 11 0.00% 31 11 0.00% 32 24 0.00% 33 15 0.00% 34 21 0.00% 35 24 0.00% 36 30 0.00% 37 28 0.00% 38 23 0.00% 39 34 0.00% 40 37 0.00% 41 51 0.00% 42 51 0.00% 43 56 0.00% 44 64 0.00% 45 58 0.00% 46 64 0.00% 47 78 0.00% 48 80 0.00% 49 100 0.00% 50 111 0.00% 51 137 0.00% 52 205 0.00% 53 197 0.00% 54 195 0.00% 55 229 0.00% 56 269 0.00% 57 309 0.00% 58 399 0.00% 59 376 0.00% 60 453 0.00% 61 618 0.00% 62 715 0.00% 63 770 0.00% 64 856 0.00% 65 1002 0.01% 66 1051 0.01% 67 1211 0.01% 68 1452 0.01% 69 1473 0.01% 70 1712 0.01% 71 1928 0.01% 72 2360 0.01% 73 2650 0.01% 74 2789 0.01% 75 3318 0.02% 76 3578 0.02% 77 3959 0.02% 78 4572 0.02% 79 5233 0.03% 80 6038 0.03% 81 6417 0.03% 82 7218 0.04% 83 8089 0.04% 84 8811 0.05% 85 10900 0.06% 86 11385 0.06% 87 11817 0.06% 88 13742 0.07% 89 14339 0.07% 90 15811 0.08% 91 16714 0.09% 92 19259 0.10% 93 20242 0.10% 94 21135 0.11% 95 24038 0.12% 96 23840 0.12% 97 26234 0.13% 98 27556 0.14% 99 29663 0.15% 100 29788 0.15% 101 33705 0.17% 102 33745 0.17% 103 34197 0.18% 104 35971 0.19% 105 36818 0.19% 106 38445 0.20% 107 41026 0.21% 108 43628 0.22% 109 45918 0.24% 110 45600 0.23% 111 46682 0.24% 112 48022 0.25% 113 47609 0.24% 114 50568 0.26% 115 54582 0.28% 116 55748 0.29% 117 54298 0.28% 118 55346 0.28% 119 55190 0.28% 120 59560 0.31% 121 61289 0.32% 122 63008 0.32% 123 66976 0.34% 124 66340 0.34% 125 71512 0.37% 126 69605 0.36% 127 72103 0.37% 128 71580 0.37% 129 74572 0.38% 130 73003 0.38% 131 76881 0.40% 132 77693 0.40% 133 79033 0.41% 134 82267 0.42% 135 83737 0.43% 136 87727 0.45% 137 88645 0.46% 138 95002 0.49% 139 98615 0.51% 140 101432 0.52% 141 113741 0.59% 142 118256 0.61% 143 130380 0.67% 144 145083 0.75% 145 170470 0.88% 146 199800 1.03% 147 250686 1.29% 148 370554 1.91% 149 674255 3.47% 150 4118014 21.19% 151 10268608 52.83% 19435629 reads passed initial QC criterion=sequence-density sequence-density=1.49 sequence-density-rank=1 fanout-score=3.26 fanout-score-rank=17 prefix-density=2.51 prefix-fanout=1.9 sequence=ATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTGGACTTTAGCCATCTAGGGTGCGGCACTCAACCGCTTCGCCTAGCAGCACAACGCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAAAGGTTGACCTATTTGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTTGCTACGCCCTTCCTCGTCTCTGGGTGCCTAGGTATCCACCGCAAGCCTTTCCTCTTTTGAACCTCGCCATTAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=30 fanout-score=10.67 fanout-score-rank=1 prefix-density=0.66 prefix-fanout=1.2 sequence=GCATCGCCGGCCCCCATCCGCTTCCCTCCCGGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTCGCTATCGG criterion=sequence-density sequence-density=1.18 sequence-density-rank=1 fanout-score=5.98 fanout-score-rank=5 prefix-density=4.20 prefix-fanout=1.7 sequence=TGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGCAGCTAGCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=30 fanout-score=69.00 fanout-score-rank=1 prefix-density=1.15 prefix-fanout=1.1 sequence=AGAAGGGGTGCCCCCTCACAAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGTAAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGAACTGATGACAGGGAAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAAC SRR6941621 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 13:34:21 Started mapping on | Dec 06 13:34:21 Finished on | Dec 06 13:35:53 Mapping speed, Million of reads per hour | 760.52 Number of input reads | 19435629 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 8408305 Uniquely mapped reads % | 43.26% Average mapped length | 294.81 Number of splices: Total | 1019665 Number of splices: Annotated (sjdb) | 914760 Number of splices: GT/AG | 968462 Number of splices: GC/AG | 11995 Number of splices: AT/AC | 3821 Number of splices: Non-canonical | 35387 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.02% Deletion average length | 1.87 Insertion rate per base | 0.01% Insertion average length | 2.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 7166915 % of reads mapped to multiple loci | 36.88% Number of reads mapped to too many loci | 546363 % of reads mapped to too many loci | 2.81% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.28% % of reads unmapped: other | 14.77% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3863945 3863945 3863945 N_multimapping 7166915 7166915 7166915 N_noFeature 5377632 8232011 5445755 N_ambiguous 211394 3183 105638 UnstrandedReadsAssigned:2819279 PositiveStrandReadsAssigned:173111 NegativeStrandReadsAssigned:2856912 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=149 echo kmer=145 SRR6941621 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR6941621-trimmed-pair1.fastq SRR6941621-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,435,629 reads, 5,826,556 reads pseudoaligned [quant] estimated average fragment length: 206.506 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 961 rounds 52973 SRR6941621.ke.tsv 35125 SRR6941621.se.tsv 88098 total ==> SRR6941621.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 730.859 0 0 PNS24247 1044 838.494 2.9342 0.315737 PNS24249 1928 1722.49 0 0 PNS24246 1044 838.494 2.9342 0.315737 PNS24248 1044 838.494 2.9342 0.315737 PNS24244 1471 1265.49 8.19739 0.584455 PNS24243 293 115.536 0 0 KQK14069 1603 1397.49 150.96 9.74644 KQK14071 474 276.249 4.69035 1.53193 ==> SRR6941621.se.tsv <== BRADI_1g14170v3 239 BRADI_1g53295v3 19 BRADI_1g59795v3 6 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 34 BRADI_1g74790v3 4 BRADI_1g09890v3 0 BRADI_1g77505v3 12 BRADI_1g48960v3 0 SRR6941621 completed mapping pipeline successfully