Starting /dee2/code/volunteer_pipeline.sh SRR7473322 current disk space = 1543221321728 free memory = 1597198704 SRR7473322 SRAfilesize f7a47818d8e618469f07293bac287ab2 SRR7473322.sra SRR7473322.sra file validated SRR7473322 is paired end SRR7473322 is conventional basespace SRR7473322 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473322_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.568 34.0 34.0 34.0 33.0 34.0 2 33.566 34.0 34.0 34.0 33.0 34.0 3 33.59425 34.0 34.0 34.0 33.0 34.0 4 33.51425 34.0 34.0 34.0 33.0 34.0 5 33.57575 34.0 34.0 34.0 33.0 34.0 6 37.34575 38.0 38.0 38.0 37.0 38.0 7 37.54825 38.0 38.0 38.0 37.0 38.0 8 37.57925 38.0 38.0 38.0 38.0 38.0 9 37.60875 38.0 38.0 38.0 38.0 38.0 10-14 37.54225 38.0 38.0 38.0 38.0 38.0 15-19 37.60655 38.0 38.0 38.0 38.0 38.0 20-24 37.595600000000005 38.0 38.0 38.0 38.0 38.0 25-29 37.54285 38.0 38.0 38.0 38.0 38.0 30-34 37.44985 38.0 38.0 38.0 37.8 38.0 35-39 37.36745 38.0 38.0 38.0 37.6 38.0 40-44 37.2305 38.0 38.0 38.0 37.0 38.0 45-49 37.254650000000005 38.0 38.0 38.0 37.0 38.0 50-54 37.220949999999995 38.0 38.0 38.0 37.0 38.0 55-59 37.05665 38.0 38.0 38.0 36.2 38.0 60-64 37.0787 38.0 38.0 38.0 36.2 38.0 65-69 36.77505 38.0 38.0 38.0 35.2 38.0 70-74 36.8643 38.0 38.0 38.0 36.2 38.0 75-79 36.0586 38.0 38.0 38.0 35.4 38.0 80-84 36.01035 38.0 38.0 38.0 35.0 38.0 85-89 35.95285 38.0 38.0 38.0 34.8 38.0 90-94 35.7248 38.0 38.0 38.0 34.0 38.0 95-99 35.77755 38.0 38.0 38.0 34.0 38.0 100-104 35.61095 38.0 38.0 38.0 33.4 38.0 105-109 35.50145 38.0 38.0 38.0 32.8 38.0 110-114 35.36415 38.0 38.0 38.0 32.6 38.0 115-119 35.2719 38.0 38.0 38.0 32.0 38.0 120-124 35.04 38.0 37.4 38.0 31.0 38.0 125-129 34.5985 38.0 36.0 38.0 27.4 38.0 130-134 34.2618 38.0 35.6 38.0 24.6 38.0 135-139 33.62595 38.0 34.4 38.0 21.0 38.0 140-144 33.50655 38.0 33.6 38.0 19.8 38.0 145-149 32.995250000000006 38.0 33.0 38.0 13.8 38.0 150-151 28.576375 35.0 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 1.0 8 1.0 9 1.0 10 1.0 11 2.0 12 0.0 13 10.0 14 7.0 15 10.0 16 12.0 17 5.0 18 51.0 19 39.0 20 8.0 21 7.0 22 5.0 23 7.0 24 9.0 25 10.0 26 9.0 27 19.0 28 19.0 29 31.0 30 27.0 31 47.0 32 61.0 33 81.0 34 114.0 35 192.0 36 525.0 37 2688.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.575 14.2 10.975 28.249999999999996 2 23.7 17.8 30.975 27.525 3 22.075 21.725 29.599999999999998 26.6 4 23.65 28.199999999999996 24.775 23.375 5 25.0 30.025000000000002 24.675 20.3 6 20.474999999999998 31.974999999999998 27.700000000000003 19.85 7 15.4 22.85 41.949999999999996 19.8 8 18.725 23.95 28.725 28.599999999999998 9 20.599999999999998 21.75 30.075000000000003 27.575 10-14 21.875 26.229999999999997 25.005 26.889999999999997 15-19 21.2 25.074999999999996 27.61 26.115 20-24 20.990000000000002 26.355 27.38 25.275 25-29 21.125 26.39 27.034999999999997 25.45 30-34 20.54 26.705000000000002 26.36 26.395000000000003 35-39 22.105 25.705 26.965 25.224999999999998 40-44 22.009999999999998 24.985 27.485 25.52 45-49 22.23 24.86 27.915 24.995 50-54 21.915000000000003 25.380000000000003 26.69 26.015 55-59 21.2 25.424999999999997 27.805000000000003 25.569999999999997 60-64 21.105 25.430000000000003 27.894999999999996 25.569999999999997 65-69 20.45 27.57 26.375 25.605 70-74 20.415 28.68 25.31 25.595000000000002 75-79 21.09 27.98 25.1 25.83 80-84 21.215 27.26 26.035000000000004 25.490000000000002 85-89 22.45 26.534999999999997 25.145 25.869999999999997 90-94 21.715 26.39 25.905 25.990000000000002 95-99 22.36 25.740000000000002 25.990000000000002 25.91 100-104 22.145 27.744999999999997 24.745 25.365 105-109 21.195 27.24 25.169999999999998 26.395000000000003 110-114 21.97 27.455000000000002 24.98 25.595000000000002 115-119 21.6 27.665 24.775 25.96 120-124 21.759999999999998 27.555000000000003 24.275 26.41 125-129 22.45 26.91 25.36 25.28 130-134 22.400760875006256 27.591730490063572 25.00375431746508 25.00375431746508 135-139 22.014309301045678 27.46785410516836 25.20138089758343 25.31645569620253 140-144 22.73 27.395000000000003 24.29 25.585 145-149 22.415 27.200000000000003 24.64 25.745 150-151 21.95 27.075 25.5375 25.4375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 1.5 2 0.0 3 0.0 4 0.5 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.5 19 2.0 20 1.5 21 0.5 22 1.5 23 3.0 24 2.0 25 2.0 26 4.0 27 4.5 28 9.5 29 16.0 30 24.5 31 32.5 32 35.0 33 48.0 34 60.5 35 71.0 36 84.0 37 98.0 38 117.0 39 123.0 40 132.0 41 150.0 42 153.5 43 144.5 44 146.5 45 146.5 46 148.0 47 148.0 48 142.0 49 148.0 50 150.5 51 157.5 52 168.5 53 193.5 54 197.0 55 176.5 56 144.0 57 111.5 58 99.0 59 83.0 60 62.0 61 52.0 62 41.0 63 23.5 64 19.5 65 13.5 66 12.5 67 13.0 68 12.0 69 11.0 70 9.5 71 10.0 72 9.0 73 7.5 74 5.0 75 4.0 76 2.5 77 1.5 78 1.5 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.11499999999999999 135-139 0.065 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.35 #Duplication Level Percentage of deduplicated Percentage of total 1 89.98178506375227 74.1 2 6.52701882210079 10.75 3 1.7304189435336976 4.275 4 0.607164541590771 2.0 5 0.5768063145112325 2.375 6 0.12143290831815423 0.6 7 0.030358227079538558 0.17500000000000002 8 0.12143290831815423 0.8 9 0.12143290831815423 0.8999999999999999 >10 0.15179113539769276 1.8499999999999999 >50 0.030358227079538558 2.175 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 87 2.175 TruSeq Adapter, Index 5 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC 24 0.6 TruSeq Adapter, Index 5 (100% over 50bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 16 0.4 No Hit GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 13 0.325 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 11 0.27499999999999997 No Hit GCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCT 10 0.25 No Hit GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT 9 0.22499999999999998 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 9 0.22499999999999998 No Hit GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG 9 0.22499999999999998 No Hit CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 9 0.22499999999999998 No Hit GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC 8 0.2 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 8 0.2 No Hit GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT 8 0.2 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 8 0.2 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 7 0.17500000000000002 No Hit GTTTGATTGGCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAA 6 0.15 No Hit GGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTA 6 0.15 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 6 0.15 No Hit GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC 6 0.15 No Hit GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT 5 0.125 No Hit GTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCC 5 0.125 No Hit GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTA 5 0.125 No Hit GCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCC 5 0.125 No Hit GTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGA 5 0.125 No Hit GCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGA 5 0.125 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 5 0.125 No Hit GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC 5 0.125 No Hit GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA 5 0.125 No Hit ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC 5 0.125 No Hit CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAG 5 0.125 No Hit CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC 5 0.125 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 5 0.125 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 5 0.125 No Hit GCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTT 5 0.125 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 5 0.125 No Hit AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA 5 0.125 No Hit GATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATG 5 0.125 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGCC 5 0.125 TruSeq Adapter, Index 5 (98% over 50bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.11249999999999999 0.0 0.0 0.0 0.0 60-61 0.1875 0.0 0.0 0.0 0.0 62-63 0.21250000000000002 0.0 0.0 0.0 0.0 64-65 0.275 0.0 0.0 0.0 0.0 66-67 0.275 0.0 0.0 0.0 0.0 68-69 0.2875 0.0 0.0 0.0 0.0 70-71 0.3125 0.0 0.0 0.0 0.0 72-73 0.325 0.0 0.0 0.0 0.0 74-75 0.3875 0.0 0.0 0.0 0.0 76-77 0.4 0.0 0.0 0.0 0.0 78-79 0.44999999999999996 0.0 0.0 0.0 0.0 80-81 0.55 0.0 0.0 0.0 0.0 82-83 0.6875 0.0 0.0 0.0 0.0 84-85 0.775 0.0 0.0 0.0 0.0 86-87 0.9 0.0 0.0 0.0 0.0 88-89 1.0125 0.0 0.0 0.0 0.0 90-91 1.25 0.0 0.0 0.0 0.0 92-93 1.5625 0.0 0.0 0.0 0.0 94-95 2.0374999999999996 0.0 0.0 0.0 0.0 96-97 2.45 0.0 0.0 0.0 0.0 98-99 2.8 0.0 0.0 0.0 0.0 100-101 3.2125 0.0 0.0 0.0 0.0 102-103 3.7 0.0 0.0 0.0 0.0 104-105 4.2125 0.0 0.0 0.0 0.0 106-107 4.7625 0.0 0.0 0.0 0.0 108-109 5.2 0.0 0.0 0.0 0.0 110-111 5.862500000000001 0.0 0.0 0.0 0.0 112-113 6.2 0.0 0.0 0.0 0.0 114-115 6.574999999999999 0.0 0.0 0.0 0.0 116-117 7.1625 0.0 0.0 0.0 0.0 118-119 7.6875 0.0 0.0 0.0 0.0 120-121 8.175 0.0 0.0 0.0 0.0 122-123 8.8125 0.0 0.0 0.0 0.0 124-125 9.5375 0.0 0.0 0.0 0.0 126-127 10.5 0.0 0.0 0.0 0.0 128-129 11.1125 0.0 0.0 0.0 0.0 130-131 11.712499999999999 0.0 0.0 0.0 0.0 132-133 12.287500000000001 0.0 0.0 0.0 0.0 134-135 13.05 0.0 0.0 0.0 0.0 136-137 13.75 0.0 0.0 0.0 0.0 138-139 14.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGACC 10 0.006830828 145.0 1 CCGACCC 10 0.006830828 145.0 2 AGATGCC 25 8.7132835E-4 87.0 6 >>END_MODULE SRR7473322 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473322_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.9705 34.0 33.0 34.0 32.0 34.0 2 33.001 34.0 33.0 34.0 32.0 34.0 3 32.946 34.0 33.0 34.0 32.0 34.0 4 32.6325 34.0 33.0 34.0 32.0 34.0 5 32.85725 34.0 33.0 34.0 32.0 34.0 6 36.32125 38.0 38.0 38.0 35.0 38.0 7 36.80275 38.0 38.0 38.0 36.0 38.0 8 36.7095 38.0 38.0 38.0 36.0 38.0 9 36.74175 38.0 38.0 38.0 36.0 38.0 10-14 36.73995 38.0 38.0 38.0 36.8 38.0 15-19 36.7285 38.0 38.0 38.0 36.8 38.0 20-24 36.7295 38.0 38.0 38.0 36.4 38.0 25-29 36.78959999999999 38.0 38.0 38.0 37.0 38.0 30-34 36.706 38.0 38.0 38.0 37.0 38.0 35-39 36.681799999999996 38.0 38.0 38.0 36.8 38.0 40-44 36.64725 38.0 38.0 38.0 37.0 38.0 45-49 36.52835 38.0 38.0 38.0 35.8 38.0 50-54 36.568349999999995 38.0 38.0 38.0 36.6 38.0 55-59 36.689750000000004 38.0 38.0 38.0 37.0 38.0 60-64 36.690149999999996 38.0 38.0 38.0 36.8 38.0 65-69 36.27285 38.0 38.0 38.0 35.6 38.0 70-74 35.57985 38.0 38.0 38.0 34.2 38.0 75-79 35.547399999999996 38.0 38.0 38.0 34.2 38.0 80-84 35.5079 38.0 38.0 38.0 34.0 38.0 85-89 35.38405 38.0 38.0 38.0 33.6 38.0 90-94 35.241499999999995 38.0 38.0 38.0 32.8 38.0 95-99 35.13165 38.0 38.0 38.0 32.0 38.0 100-104 35.0093 38.0 38.0 38.0 31.2 38.0 105-109 34.7865 38.0 38.0 38.0 29.0 38.0 110-114 34.30735 38.0 36.8 38.0 23.2 38.0 115-119 34.40124999999999 38.0 37.0 38.0 25.2 38.0 120-124 33.79445 38.0 35.8 38.0 22.0 38.0 125-129 33.83385 38.0 35.8 38.0 20.6 38.0 130-134 33.4965 38.0 35.0 38.0 18.6 38.0 135-139 33.20235 38.0 34.6 38.0 13.6 38.0 140-144 32.39365 38.0 33.0 38.0 11.8 38.0 145-149 31.3807 38.0 33.0 38.0 2.0 38.0 150-151 26.25175 33.0 17.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 9.0 4 7.0 5 5.0 6 6.0 7 2.0 8 1.0 9 6.0 10 17.0 11 18.0 12 5.0 13 5.0 14 7.0 15 13.0 16 16.0 17 73.0 18 9.0 19 5.0 20 9.0 21 4.0 22 8.0 23 7.0 24 11.0 25 18.0 26 20.0 27 14.0 28 26.0 29 34.0 30 31.0 31 43.0 32 59.0 33 88.0 34 114.0 35 196.0 36 568.0 37 2525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.425 21.6 11.05 20.925 2 29.849999999999998 22.575 26.75 20.825 3 24.60615153788447 24.50612653163291 29.982495623905976 20.905226306576644 4 27.650000000000002 31.7 19.825 20.825 5 30.582645661415352 32.65816454113528 18.02950737684421 18.72968242060515 6 25.650000000000002 34.775 19.625 19.950000000000003 7 22.525000000000002 22.55 32.574999999999996 22.35 8 24.45 25.55 22.425 27.575 9 26.075 24.7 23.925 25.3 10-14 27.42 25.85 22.36 24.37 15-19 27.089999999999996 25.53 24.84 22.54 20-24 28.325 26.395000000000003 23.535 21.745 25-29 27.195000000000004 27.560000000000002 23.405 21.84 30-34 28.560000000000002 26.290000000000003 24.135 21.015 35-39 25.81 25.330000000000002 25.435000000000002 23.425 40-44 28.78 25.825 23.91 21.485000000000003 45-49 26.645000000000003 24.955 25.545 22.855 50-54 25.485000000000003 25.995 25.979999999999997 22.54 55-59 25.345000000000002 26.915 25.945 21.795 60-64 25.0 28.060000000000002 24.955 21.985 65-69 25.91 28.505000000000003 24.785 20.8 70-74 26.334999999999997 27.794999999999998 24.77 21.099999999999998 75-79 25.585 27.515 24.79 22.11 80-84 26.490000000000002 27.339999999999996 25.169999999999998 21.0 85-89 26.025 28.005000000000003 24.6 21.37 90-94 26.400000000000002 27.725 24.91 20.965 95-99 25.71 28.095 24.87 21.325 100-104 27.034999999999997 27.785 24.33 20.849999999999998 105-109 26.185000000000002 28.645 23.735 21.435000000000002 110-114 26.580316063212646 28.465693138627724 23.73974794958992 21.214242848569715 115-119 26.762676267626762 29.332933293329333 23.392339233923394 20.512051205120514 120-124 26.21 28.610000000000003 24.19 20.990000000000002 125-129 26.44 28.665000000000003 24.45 20.445 130-134 26.525 28.144999999999996 24.305 21.025 135-139 26.905 28.025 24.55 20.52 140-144 27.075 27.095000000000002 24.755 21.075 145-149 28.04 27.54 24.03 20.39 150-151 27.700000000000003 28.8625 24.0 19.4375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.5 22 2.0 23 1.0 24 1.0 25 4.5 26 5.5 27 8.0 28 12.5 29 13.5 30 18.5 31 26.0 32 25.5 33 30.5 34 48.0 35 52.5 36 71.0 37 94.0 38 103.5 39 117.5 40 135.0 41 127.5 42 112.5 43 114.0 44 116.0 45 118.0 46 123.5 47 129.5 48 145.0 49 160.5 50 159.5 51 157.5 52 174.0 53 226.5 54 232.0 55 201.5 56 178.5 57 133.5 58 101.0 59 90.5 60 72.5 61 51.5 62 53.5 63 46.0 64 28.0 65 26.5 66 25.0 67 21.5 68 17.5 69 14.5 70 13.0 71 11.5 72 8.0 73 6.5 74 7.0 75 4.5 76 4.0 77 4.0 78 3.5 79 3.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.025 4 0.0 5 0.025 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.02 115-119 0.01 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.55 #Duplication Level Percentage of deduplicated Percentage of total 1 91.35248354278875 76.325 2 5.475763016157989 9.15 3 1.526032315978456 3.8249999999999997 4 0.5984440454817475 2.0 5 0.3590664272890485 1.5 6 0.26929982046678635 1.35 7 0.029922202274087373 0.17500000000000002 8 0.059844404548174746 0.4 9 0.029922202274087373 0.22499999999999998 >10 0.26929982046678635 2.875 >50 0.029922202274087373 2.175 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 87 2.175 Illumina Single End PCR Primer 1 (100% over 50bp) GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 20 0.5 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 16 0.4 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 15 0.375 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 12 0.3 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 11 0.27499999999999997 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 11 0.27499999999999997 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 10 0.25 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 10 0.25 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 10 0.25 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 9 0.22499999999999998 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 8 0.2 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 8 0.2 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 7 0.17500000000000002 No Hit CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 6 0.15 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 6 0.15 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 6 0.15 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 6 0.15 No Hit GGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATG 6 0.15 No Hit GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGC 6 0.15 No Hit ACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA 6 0.15 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 6 0.15 No Hit GCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGT 6 0.15 No Hit AATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCA 5 0.125 No Hit GTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTT 5 0.125 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 5 0.125 No Hit AAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA 5 0.125 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 5 0.125 No Hit GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC 5 0.125 No Hit AGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCAAAA 5 0.125 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 5 0.125 No Hit GGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTA 5 0.125 No Hit GGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCG 5 0.125 No Hit GTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA 5 0.125 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.16249999999999998 0.0 0.0 0.0 0.0 62-63 0.1875 0.0 0.0 0.0 0.0 64-65 0.25 0.0 0.0 0.0 0.0 66-67 0.25 0.0 0.0 0.0 0.0 68-69 0.2625 0.0 0.0 0.0 0.0 70-71 0.2875 0.0 0.0 0.0 0.0 72-73 0.3 0.0 0.0 0.0 0.0 74-75 0.3625 0.0 0.0 0.0 0.0 76-77 0.375 0.0 0.0 0.0 0.0 78-79 0.42500000000000004 0.0 0.0 0.0 0.0 80-81 0.5125 0.0 0.0 0.0 0.0 82-83 0.6625000000000001 0.0 0.0 0.0 0.0 84-85 0.725 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 0.9125 0.0 0.0 0.0 0.0 90-91 1.1749999999999998 0.0 0.0 0.0 0.0 92-93 1.5125 0.0 0.0 0.0 0.0 94-95 1.975 0.0 0.0 0.0 0.0 96-97 2.45 0.0 0.0 0.0 0.0 98-99 2.7750000000000004 0.0 0.0 0.0 0.0 100-101 3.15 0.0 0.0 0.0 0.0 102-103 3.6375 0.0 0.0 0.0 0.0 104-105 4.15 0.0 0.0 0.0 0.0 106-107 4.65 0.0 0.0 0.0 0.0 108-109 5.125 0.0 0.0 0.0 0.0 110-111 5.75 0.0 0.0 0.0 0.0 112-113 6.075 0.0 0.0 0.0 0.0 114-115 6.4125 0.0 0.0 0.0 0.0 116-117 6.975 0.0 0.0 0.0 0.0 118-119 7.5 0.0 0.0 0.0 0.0 120-121 7.95 0.0 0.0 0.0 0.0 122-123 8.600000000000001 0.0 0.0 0.0 0.0 124-125 9.35 0.0 0.0 0.0 0.0 126-127 10.2875 0.0 0.0 0.0 0.0 128-129 10.8375 0.0 0.0 0.0 0.0 130-131 11.425 0.0 0.0 0.0 0.0 132-133 11.975 0.0 0.0 0.0 0.0 134-135 12.825 0.0 0.0 0.0 0.0 136-137 13.5125 0.0 0.0 0.0 0.0 138-139 14.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCCCA 10 0.006830828 145.0 6 CCCAGAG 10 0.006830828 145.0 9 GGGGGGG 20 0.00593511 29.0 100-104 >>END_MODULE Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922378 spots for SRR7473322.sra Written 922378 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra Read 922372 spots for SRR7473322.sra Written 922372 spots for SRR7473322.sra SRR ids: ['SRR7473322.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_of_5eo_r SRR7473322.sra spots: 18447446 blocks: [[1, 922372], [922373, 1844744], [1844745, 2767116], [2767117, 3689488], [3689489, 4611860], [4611861, 5534232], [5534233, 6456604], [6456605, 7378976], [7378977, 8301348], [8301349, 9223720], [9223721, 10146092], [10146093, 11068464], [11068465, 11990836], [11990837, 12913208], [12913209, 13835580], [13835581, 14757952], [14757953, 15680324], [15680325, 16602696], [16602697, 17525068], [17525069, 18447446]] SRR7473322 file size 6229533 SRR7473322 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473322 SRR7473322_1.fastq SRR7473322_2.fastq Input file: SRR7473322_1.fastq Paired file: SRR7473322_2.fastq trimmed: SRR7473322-trimmed-pair1.fastq, SRR7473322-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 13:19:22 2024 >> started Sat Dec 7 13:19:44 2024 >> done (22.619s) 18447446 read pairs processed; of these: 81433 ( 0.44%) short read pairs filtered out after trimming by size control 477984 ( 2.59%) empty read pairs filtered out after trimming by size control 17888029 (96.97%) read pairs available; of these: 9765358 (54.59%) trimmed read pairs available after processing 8122671 (45.41%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 51 0.00% 20 30 0.00% 21 33 0.00% 22 44 0.00% 23 47 0.00% 24 32 0.00% 25 27 0.00% 26 40 0.00% 27 88 0.00% 28 75 0.00% 29 77 0.00% 30 101 0.00% 31 87 0.00% 32 81 0.00% 33 88 0.00% 34 124 0.00% 35 135 0.00% 36 129 0.00% 37 135 0.00% 38 210 0.00% 39 241 0.00% 40 299 0.00% 41 324 0.00% 42 358 0.00% 43 372 0.00% 44 492 0.00% 45 612 0.00% 46 722 0.00% 47 796 0.00% 48 880 0.00% 49 1145 0.01% 50 1517 0.01% 51 1542 0.01% 52 1622 0.01% 53 1624 0.01% 54 1626 0.01% 55 1756 0.01% 56 2254 0.01% 57 2386 0.01% 58 2082 0.01% 59 1748 0.01% 60 2039 0.01% 61 2408 0.01% 62 2470 0.01% 63 2841 0.02% 64 3304 0.02% 65 4638 0.03% 66 5079 0.03% 67 7345 0.04% 68 12680 0.07% 69 57473 0.32% 70 93308 0.52% 71 32968 0.18% 72 17126 0.10% 73 13705 0.08% 74 11858 0.07% 75 11169 0.06% 76 10322 0.06% 77 10801 0.06% 78 11189 0.06% 79 12622 0.07% 80 13315 0.07% 81 14314 0.08% 82 17429 0.10% 83 19803 0.11% 84 24873 0.14% 85 25921 0.14% 86 27629 0.15% 87 28113 0.16% 88 29896 0.17% 89 29466 0.16% 90 32078 0.18% 91 35534 0.20% 92 34654 0.19% 93 41528 0.23% 94 43237 0.24% 95 44650 0.25% 96 44433 0.25% 97 40810 0.23% 98 39713 0.22% 99 41017 0.23% 100 46716 0.26% 101 42990 0.24% 102 45759 0.26% 103 48928 0.27% 104 52336 0.29% 105 58197 0.33% 106 53334 0.30% 107 50556 0.28% 108 53500 0.30% 109 64932 0.36% 110 63811 0.36% 111 54450 0.30% 112 57339 0.32% 113 70605 0.39% 114 64707 0.36% 115 69682 0.39% 116 67213 0.38% 117 62431 0.35% 118 63678 0.36% 119 61295 0.34% 120 65195 0.36% 121 63765 0.36% 122 66863 0.37% 123 71716 0.40% 124 76896 0.43% 125 74446 0.42% 126 73579 0.41% 127 72741 0.41% 128 72333 0.40% 129 73883 0.41% 130 75568 0.42% 131 75272 0.42% 132 79500 0.44% 133 85303 0.48% 134 89422 0.50% 135 94610 0.53% 136 95906 0.54% 137 100214 0.56% 138 102153 0.57% 139 101862 0.57% 140 102478 0.57% 141 114001 0.64% 142 117772 0.66% 143 128842 0.72% 144 141858 0.79% 145 162823 0.91% 146 192684 1.08% 147 242571 1.36% 148 351728 1.97% 149 693092 3.87% 150 3774016 21.10% 151 8122671 45.41% 17888029 reads passed initial QC criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=5.52 fanout-score-rank=17 prefix-density=1.63 prefix-fanout=1.8 sequence=TCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=27.48 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=5.5 sequence=TTTTTTTTTATGTGCCTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAAAAAGTGGCAAAAACAGGCAAGCCGGTGATTTTATCTACAGGAATGTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATGGATGCAGGGATATTATTTTATTGCATTGTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTGACCATACATTGGGAATACTCGCCCCAGTAGTTTCTGTTGCCTTAGGAGCGGATGTTATTGAGAAGCACTTTACCTTAGATAAAAATATGGAAGGTCCTGATCATGCTTTGTCAGCAGACCCAGAAGAATTTAAGGAAATGGTTAATAACATAAGATTAGTTGAAAAAATGCTTGGAAGTGGGGAAAAGATACCAATGCCTTCTGAAAGAGACGTTATTGTTGAAGC criterion=sequence-density sequence-density=2.51 sequence-density-rank=1 fanout-score=2.35 fanout-score-rank=29 prefix-density=2.69 prefix-fanout=2.2 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.17 sequence-density-rank=24 fanout-score=17.33 fanout-score-rank=1 prefix-density=2.53 prefix-fanout=1.2 sequence=AACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAGCGATTCAGCGCTATTTGCGCCAAGAACTGTTAACGTCTTGAATTCTGG SRR7473322 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 13:21:29 Started mapping on | Dec 07 13:21:29 Finished on | Dec 07 13:46:52 Mapping speed, Million of reads per hour | 42.28 Number of input reads | 17888029 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 8367220 Uniquely mapped reads % | 46.78% Average mapped length | 289.14 Number of splices: Total | 8039388 Number of splices: Annotated (sjdb) | 7422564 Number of splices: GT/AG | 7930728 Number of splices: GC/AG | 94517 Number of splices: AT/AC | 4973 Number of splices: Non-canonical | 9170 Mismatch rate per base, % | 0.13% Deletion rate per base | 0.01% Deletion average length | 1.46 Insertion rate per base | 0.01% Insertion average length | 1.82 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 162831 % of reads mapped to multiple loci | 0.91% Number of reads mapped to too many loci | 10535 % of reads mapped to too many loci | 0.06% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 50.24% % of reads unmapped: other | 2.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9379548 9379548 9379548 N_multimapping 162831 162831 162831 N_noFeature 363842 8052373 461134 N_ambiguous 263121 1769 45717 UnstrandedReadsAssigned:7740257 PositiveStrandReadsAssigned:313078 NegativeStrandReadsAssigned:7860369 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=140 echo kmer=135 SRR7473322 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473322-trimmed-pair1.fastq SRR7473322-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,888,029 reads, 8,079,199 reads pseudoaligned [quant] estimated average fragment length: 240.835 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,051 rounds 52973 SRR7473322.ke.tsv 35125 SRR7473322.se.tsv 88098 total ==> SRR7473322.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 696.733 5.55199e-05 1.10849e-05 PNS24247 1044 804.165 18.6966 3.23419 PNS24249 1928 1688.17 11.082 0.913176 PNS24246 1044 804.165 18.6966 3.23419 PNS24248 1044 804.165 18.6966 3.23419 PNS24244 1471 1231.17 32.8282 3.7092 PNS24243 293 105.113 0 0 KQK14069 1603 1363.17 4177.6 426.312 KQK14071 474 251.435 123.839 68.5145 ==> SRR7473322.se.tsv <== BRADI_1g14170v3 4673 BRADI_1g53295v3 32 BRADI_1g59795v3 323 BRADI_1g07683v3 0 BRADI_1g00485v3 30 BRADI_1g20270v3 1032 BRADI_1g74790v3 75 BRADI_1g09890v3 0 BRADI_1g77505v3 381 BRADI_1g48960v3 0 SRR7473322 completed mapping pipeline successfully