Starting /dee2/code/volunteer_pipeline.sh SRR7473323 current disk space = 1543210078208 free memory = 1596485224 SRR7473323 SRAfilesize fc28d1834f23f30947777fdf5b82b233 SRR7473323.sra SRR7473323.sra file validated SRR7473323 is paired end SRR7473323 is conventional basespace SRR7473323 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473323_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.2615 34.0 33.0 34.0 32.0 34.0 2 33.285 34.0 33.0 34.0 31.0 34.0 3 33.20225 34.0 33.0 34.0 31.0 34.0 4 32.85625 34.0 33.0 34.0 32.0 34.0 5 33.231 34.0 33.0 34.0 32.0 34.0 6 36.35 38.0 37.0 38.0 33.0 38.0 7 37.03425 38.0 38.0 38.0 36.0 38.0 8 36.95925 38.0 38.0 38.0 35.0 38.0 9 37.16025 38.0 38.0 38.0 36.0 38.0 10-14 37.176100000000005 38.0 38.0 38.0 36.8 38.0 15-19 37.189 38.0 38.0 38.0 36.6 38.0 20-24 37.03 38.0 38.0 38.0 36.4 38.0 25-29 37.05305 38.0 38.0 38.0 36.2 38.0 30-34 36.87645 38.0 38.0 38.0 35.8 38.0 35-39 36.64085 38.0 38.0 38.0 34.4 38.0 40-44 35.09335 38.0 36.2 38.0 26.0 38.0 45-49 36.197900000000004 38.0 38.0 38.0 33.0 38.0 50-54 35.85265 38.0 37.8 38.0 30.0 38.0 55-59 36.1995 38.0 38.0 38.0 33.2 38.0 60-64 35.52795 38.0 37.4 38.0 29.4 38.0 65-69 34.699600000000004 38.0 36.6 38.0 20.4 38.0 70-74 33.1002 38.0 31.8 38.0 17.4 38.0 75-79 27.6101 38.0 14.8 38.0 2.0 38.0 80-84 27.34715 38.0 13.6 38.0 2.0 38.0 85-89 27.424650000000003 38.0 8.8 38.0 2.0 38.0 90-94 27.085649999999998 38.0 2.0 38.0 2.0 38.0 95-99 26.55035 38.0 2.0 38.0 2.0 38.0 100-104 26.106099999999998 38.0 2.0 38.0 2.0 38.0 105-109 25.3089 37.0 2.0 38.0 2.0 38.0 110-114 25.1323 36.8 2.0 38.0 2.0 38.0 115-119 24.706899999999997 36.2 2.0 38.0 2.0 38.0 120-124 24.51785 36.0 2.0 38.0 2.0 38.0 125-129 23.8558 35.2 2.0 38.0 2.0 38.0 130-134 23.141000000000002 34.0 2.0 38.0 2.0 38.0 135-139 22.6347 33.2 2.0 38.0 2.0 38.0 140-144 22.2327 33.0 2.0 38.0 2.0 38.0 145-149 21.28845 31.2 2.0 38.0 2.0 38.0 150-151 17.706375 15.5 2.0 35.5 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 2.0 7 0.0 8 5.0 9 5.0 10 14.0 11 11.0 12 21.0 13 32.0 14 68.0 15 83.0 16 72.0 17 107.0 18 288.0 19 333.0 20 31.0 21 45.0 22 59.0 23 62.0 24 45.0 25 29.0 26 40.0 27 58.0 28 61.0 29 42.0 30 43.0 31 55.0 32 51.0 33 69.0 34 92.0 35 189.0 36 517.0 37 1470.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.748433976447004 16.762716111250313 11.826609872212478 27.662240040090204 2 17.05 35.05 28.15 19.75 3 16.85 15.85 39.75 27.55 4 24.85 18.625 14.875 41.65 5 48.4 22.5 14.625 14.475 6 39.675 30.45 15.7 14.174999999999999 7 11.700000000000001 47.15 27.375 13.775 8 20.25 41.8 20.150000000000002 17.8 9 37.15 23.1 21.4 18.35 10-14 21.455 31.35 16.975 30.220000000000002 15-19 21.125 22.814999999999998 25.979999999999997 30.080000000000002 20-24 20.7 31.04 24.145 24.115000000000002 25-29 20.65 24.72 24.325 30.305 30-34 15.024999999999999 32.385000000000005 22.24 30.349999999999998 35-39 27.277727772777276 31.373137313731377 17.68176817681768 23.667366736673667 40-44 16.17566148151853 23.878357425098784 29.80543190116541 30.140549192217275 45-49 25.648847327099066 24.818722808421263 30.344551682752414 19.187878181727257 50-54 21.785 18.32 25.285000000000004 34.61 55-59 21.165 18.265 34.86 25.71 60-64 21.92 25.785000000000004 29.880000000000003 22.415 65-69 15.47 48.795 17.28 18.455 70-74 16.81 47.75 17.175 18.265 75-79 16.335 42.185 17.715 23.765 80-84 20.17600880044002 32.366618330916545 23.646182309115456 23.811190559527976 85-89 20.215 29.880000000000003 22.384999999999998 27.52 90-94 19.71767532662562 26.200130149672123 26.27521649897382 27.80697802472844 95-99 20.32450896669513 29.28618074044306 24.23268197116592 26.156628321695884 100-104 19.755 37.925 20.815 21.505 105-109 17.854999999999997 39.58 20.765 21.8 110-114 18.715 38.525 19.830000000000002 22.93 115-119 18.77 35.225 22.775000000000002 23.23 120-124 19.139999999999997 34.33 22.32 24.21 125-129 19.47490192133588 37.15923951312745 20.837943868826073 22.527914696710592 130-134 18.157455630277592 39.18187793902007 19.568185265712696 23.092481164989636 135-139 19.23251933869255 37.52970322058749 19.70777086809242 23.530006572627535 140-144 19.861874275344054 35.44890860513183 19.816504511770933 24.87271260775319 145-149 20.861127956337175 34.82918940772185 19.749343036183546 24.560339599757427 150-151 19.66469179377285 35.547712088743225 20.67313752678684 24.114458590697087 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 1.0 26 1.5 27 2.5 28 5.0 29 7.0 30 10.0 31 15.0 32 22.5 33 25.5 34 31.0 35 45.5 36 69.0 37 102.5 38 130.5 39 145.5 40 186.5 41 223.0 42 225.5 43 225.0 44 217.0 45 206.0 46 194.0 47 197.5 48 212.5 49 192.0 50 161.5 51 147.0 52 135.0 53 131.5 54 112.0 55 87.0 56 72.0 57 64.0 58 56.0 59 45.5 60 43.0 61 34.5 62 28.5 63 29.5 64 23.5 65 14.0 66 14.0 67 19.0 68 16.5 69 12.0 70 11.5 71 11.0 72 7.5 73 5.5 74 5.0 75 3.5 76 5.0 77 6.0 78 2.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.01 40-44 0.034999999999999996 45-49 0.015 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.005 85-89 0.0 90-94 0.11499999999999999 95-99 0.46499999999999997 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.59 130-134 1.115 135-139 1.105 140-144 0.815 145-149 1.06 150-151 0.8375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.125 #Duplication Level Percentage of deduplicated Percentage of total 1 95.3072625698324 63.975 2 3.4636871508379885 4.65 3 0.33519553072625696 0.675 4 0.37243947858473 1.0 5 0.11173184357541899 0.375 6 0.0 0.0 7 0.11173184357541899 0.525 8 0.037243947858472994 0.2 9 0.037243947858472994 0.22499999999999998 >10 0.14897579143389197 2.225 >50 0.0 0.0 >100 0.037243947858472994 4.8 >500 0.037243947858472994 21.349999999999998 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATCTCGTATGC 854 21.349999999999998 TruSeq Adapter, Index 10 (97% over 37bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATCTCGTATGCC 192 4.8 TruSeq Adapter, Index 10 (97% over 36bp) AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATCTCGTATG 42 1.05 TruSeq Adapter, Index 10 (97% over 37bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAAACTCGTATGCC 18 0.44999999999999996 TruSeq Adapter, Index 10 (97% over 36bp) TCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATCTCGTATGCCG 15 0.375 TruSeq Adapter, Index 10 (97% over 35bp) CGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATCTCGTATGCCGT 14 0.35000000000000003 TruSeq Adapter, Index 10 (97% over 34bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATATCGTATGC 9 0.22499999999999998 TruSeq Adapter, Index 10 (97% over 37bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATATCGTATGCC 8 0.2 TruSeq Adapter, Index 10 (97% over 36bp) GAAGAGCACACGTCTGAACTCCAGTCACAAGCGAATCTCGTATGCCGTCT 7 0.17500000000000002 TruSeq Adapter, Index 10 (96% over 32bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCCCAAGCGAATCTCGTATGCC 7 0.17500000000000002 TruSeq Adapter, Index 1 (96% over 33bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAACCTCGTATGC 7 0.17500000000000002 TruSeq Adapter, Index 10 (97% over 37bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAACCTCGTATGCC 5 0.125 TruSeq Adapter, Index 10 (97% over 36bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGAAACTCGTATGC 5 0.125 TruSeq Adapter, Index 10 (97% over 37bp) AGAGCACACGTCTGAACTCCAGTCACAAGCGAATCTCGTATGCCGTCTTC 5 0.125 TruSeq Adapter, Index 10 (96% over 30bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1 0.0 0.0 0.0 0.0 2 1.1 0.0 0.0 0.0 0.0 3 1.1 0.0 0.0 0.0 0.0 4 1.1 0.0 0.0 0.0 0.0 5 1.1 0.0 0.0 0.0 0.0 6 1.1 0.0 0.0 0.0 0.0 7 1.1 0.0 0.0 0.0 0.0 8 1.1 0.0 0.0 0.0 0.0 9 1.1 0.0 0.0 0.0 0.0 10-11 1.15 0.0 0.0 0.0 0.0 12-13 1.2125 0.0 0.0 0.0 0.0 14-15 1.25 0.0 0.0 0.0 0.0 16-17 1.275 0.0 0.0 0.0 0.0 18-19 1.325 0.0 0.0 0.0 0.0 20-21 1.3375 0.0 0.0 0.0 0.0 22-23 1.375 0.0 0.0 0.0 0.0 24-25 1.375 0.0 0.0 0.0 0.0 26-27 1.375 0.0 0.0 0.0 0.0 28-29 1.375 0.0 0.0 0.0 0.0 30-31 1.375 0.0 0.0 0.0 0.0 32-33 1.375 0.0 0.0 0.0 0.0 34-35 1.375 0.0 0.0 0.0 0.0 36-37 1.375 0.0 0.0 0.0 0.0 38-39 1.375 0.0 0.0 0.0 0.0 40-41 1.375 0.0 0.0 0.0 0.0 42-43 1.375 0.0 0.0 0.0 0.0 44-45 1.375 0.0 0.0 0.0 0.0 46-47 1.375 0.0 0.0 0.0 0.0 48-49 1.3875 0.0 0.0 0.0 0.0 50-51 1.4125 0.0 0.0 0.0 0.0 52-53 1.4625 0.0 0.0 0.0 0.0 54-55 1.475 0.0 0.0 0.0 0.0 56-57 1.4875 0.0 0.0 0.0 0.0 58-59 1.625 0.0 0.0 0.0 0.0 60-61 1.875 0.0 0.0 0.0 0.0 62-63 1.9125 0.0 0.0 0.0 0.0 64-65 1.925 0.0 0.0 0.0 0.0 66-67 1.9625 0.0 0.0 0.0 0.0 68-69 2.05 0.0 0.0 0.0 0.0 70-71 2.0875000000000004 0.0 0.0 0.0 0.0 72-73 2.1875 0.0 0.0 0.0 0.0 74-75 2.2 0.0 0.0 0.0 0.0 76-77 2.2375 0.0 0.0 0.0 0.0 78-79 2.3125 0.0 0.0 0.0 0.0 80-81 2.4625000000000004 0.0 0.0 0.0 0.0 82-83 2.625 0.0 0.0 0.0 0.0 84-85 2.7375 0.0 0.0 0.0 0.0 86-87 2.8 0.0 0.0 0.0 0.0 88-89 2.95 0.0 0.0 0.0 0.0 90-91 3.05 0.0 0.0 0.0 0.0 92-93 3.2750000000000004 0.0 0.0 0.0 0.0 94-95 3.4000000000000004 0.0 0.0 0.0 0.0 96-97 3.6500000000000004 0.0 0.0 0.0 0.0 98-99 3.9000000000000004 0.0 0.0 0.0 0.0 100-101 4.137499999999999 0.0 0.0 0.0 0.0 102-103 4.5 0.0 0.0 0.0 0.0 104-105 4.75 0.0 0.0 0.0 0.0 106-107 5.15 0.0 0.0 0.0 0.0 108-109 5.4 0.0 0.0 0.0 0.0 110-111 5.7875 0.0 0.0 0.0 0.0 112-113 6.1 0.0 0.0 0.0 0.0 114-115 6.550000000000001 0.0 0.0 0.0 0.0 116-117 7.0 0.0 0.0 0.0 0.0 118-119 7.324999999999999 0.0 0.0 0.0 0.0 120-121 7.5875 0.0 0.0 0.0 0.0 122-123 7.925 0.0 0.0 0.0 0.0 124-125 8.1375 0.0 0.0 0.0 0.0 126-127 8.462499999999999 0.0 0.0 0.0 0.0 128-129 9.025 0.0 0.0 0.0 0.0 130-131 9.45 0.0 0.0 0.0 0.0 132-133 10.0875 0.0 0.0 0.0 0.0 134-135 10.6375 0.0 0.0 0.0 0.0 136-137 11.325 0.0 0.0 0.0 0.0 138-139 11.837499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGGTT 10 0.006901744 144.5 1 GATCGGA 140 0.0 82.57143 1 TCGGAAG 160 0.0 72.25 3 ATCGGAA 160 0.0 72.25 2 GGAAGAG 165 0.0 70.06061 5 CGGAAGA 170 0.0 68.0 4 GAGCACA 175 0.0 66.057144 9 AGAGCAC 180 0.0 64.22222 8 GAAGAGC 185 0.0 62.48649 6 AAGAGCA 195 0.0 59.282047 7 TGCTTGA 150 0.0 21.193333 55-59 GCTTGAA 150 0.0 21.193333 55-59 TGCCGTC 160 0.0 20.771875 45-49 CAAGCGA 165 0.0 20.142424 30-34 GAATCTC 155 0.0 19.57742 35-39 TCGTATG 155 0.0 19.57742 40-44 CGTATGC 150 0.0 19.266666 40-44 ACTCCAG 175 0.0 18.991428 20-24 AGTCACA 175 0.0 18.991428 25-29 GAAAAAA 160 0.0 18.965626 60-64 >>END_MODULE SRR7473323 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473323_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.06025 33.0 32.0 34.0 18.0 34.0 2 30.784 33.0 32.0 34.0 27.0 34.0 3 29.90075 33.0 32.0 34.0 18.0 34.0 4 30.186 33.0 32.0 34.0 15.0 34.0 5 30.094 33.0 32.0 34.0 15.0 34.0 6 33.541 38.0 36.0 38.0 16.0 38.0 7 34.34775 38.0 37.0 38.0 26.0 38.0 8 34.3105 38.0 37.0 38.0 16.0 38.0 9 33.98925 38.0 36.0 38.0 16.0 38.0 10-14 33.686150000000005 38.0 36.8 38.0 15.6 38.0 15-19 33.10035 38.0 36.2 38.0 14.8 38.0 20-24 33.21745 38.0 36.6 38.0 14.8 38.0 25-29 32.9405 38.0 36.2 38.0 15.6 38.0 30-34 32.56085 38.0 35.2 38.0 15.0 38.0 35-39 32.4011 38.0 35.0 38.0 15.2 38.0 40-44 32.7014 38.0 35.4 38.0 14.2 38.0 45-49 32.04765 38.0 33.4 38.0 14.0 38.0 50-54 32.0944 38.0 33.0 38.0 14.2 38.0 55-59 32.4705 38.0 35.0 38.0 15.0 38.0 60-64 33.0582 38.0 36.2 38.0 14.2 38.0 65-69 30.2623 38.0 25.0 38.0 2.0 38.0 70-74 25.46105 38.0 2.0 38.0 2.0 38.0 75-79 24.874550000000003 38.0 2.0 38.0 2.0 38.0 80-84 24.73605 38.0 2.0 38.0 2.0 38.0 85-89 24.578899999999997 38.0 2.0 38.0 2.0 38.0 90-94 24.375 38.0 2.0 38.0 2.0 38.0 95-99 23.9572 37.8 2.0 38.0 2.0 38.0 100-104 23.463150000000002 37.0 2.0 38.0 2.0 38.0 105-109 23.30245 36.4 2.0 38.0 2.0 38.0 110-114 23.06255 36.0 2.0 38.0 2.0 38.0 115-119 22.916549999999997 35.4 2.0 38.0 2.0 38.0 120-124 22.812 35.0 2.0 38.0 2.0 38.0 125-129 22.47335 33.4 2.0 38.0 2.0 38.0 130-134 21.998600000000003 33.0 2.0 38.0 2.0 38.0 135-139 21.5303 33.0 2.0 38.0 2.0 38.0 140-144 21.014249999999997 30.6 2.0 38.0 2.0 38.0 145-149 20.081049999999998 27.4 2.0 38.0 2.0 38.0 150-151 16.274125 2.0 2.0 34.5 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 167.0 3 77.0 4 27.0 5 24.0 6 18.0 7 5.0 8 8.0 9 15.0 10 48.0 11 127.0 12 85.0 13 34.0 14 56.0 15 63.0 16 120.0 17 512.0 18 47.0 19 32.0 20 27.0 21 18.0 22 23.0 23 16.0 24 26.0 25 31.0 26 18.0 27 16.0 28 18.0 29 33.0 30 34.0 31 39.0 32 55.0 33 66.0 34 81.0 35 156.0 36 442.0 37 1436.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.10913253625032 22.360722462477742 13.7624014245739 22.76774357669804 2 21.041931385006354 41.905972045743326 22.744599745870396 14.307496823379923 3 25.609756097560975 16.920731707317074 38.03353658536585 19.4359756097561 4 22.34069560802234 20.436659050520436 14.876872302614878 42.345773038842346 5 46.370558375634516 24.18781725888325 14.847715736040609 14.593908629441623 6 47.11881693013768 24.604793472718 14.227434982151962 14.04895461499235 7 17.03534197813374 43.630816170861934 24.10373760488177 15.230104246122552 8 19.893697798025816 44.82409516578081 16.856492027334852 18.425715008858518 9 43.86541866936504 21.376169997470278 18.239311915001267 16.51909941816342 10-14 31.640585562847047 22.86723876829884 19.989904088844018 25.502271580010095 15-19 31.59233077353405 20.22580481106647 27.116920103748154 21.064944311651324 20-24 36.60144076023093 29.09109487559393 18.11168446329127 16.19577990088387 25-29 30.57948116813618 34.070638601498395 18.791091177819684 16.55878905254574 30-34 32.73718536635076 24.747783552430448 26.266177519616836 16.248853561601955 35-39 22.463361078486443 23.106776285553796 28.146862074248073 26.283000561711688 40-44 41.912139435327695 18.097033941494242 23.845683416573234 16.145143206604832 45-49 27.817045687359148 17.634706002868263 22.346855152632656 32.20139315713993 50-54 24.696707105719238 23.42236721378326 28.963197063920887 22.917728616576614 55-59 18.603465851172274 33.94495412844037 30.152905198776757 17.2986748216106 60-64 18.643721595261194 48.21018230097533 17.459020579073687 15.687075524689782 65-69 19.78700416730977 47.389000360137885 17.374080362195812 15.449915110356535 70-74 22.290784843694222 42.79157529705747 17.752052628894894 17.16558723035341 75-79 24.524743896845216 36.399775750471434 20.35064471739463 18.724835635288724 80-84 26.783893985728845 34.918450560652396 21.30479102956167 16.992864424057085 85-89 25.721117159281683 35.32075087754998 21.478353767105865 17.47977819606247 90-94 27.385870951287938 30.986993114001532 23.218566692170363 18.408569242540167 95-99 25.29829253240074 32.812178564081464 23.097099362271138 18.79242954124666 100-104 27.476606200010455 31.564640075278373 22.191437085054105 18.767316639657064 105-109 25.79164275653399 32.860347435964314 20.992227033230737 20.35578277427096 110-114 27.338204592901878 31.033402922755744 21.758872651356995 19.869519832985386 115-119 23.353759867054425 32.182176983797255 24.350851682592438 20.113211466555878 120-124 22.928578854350764 35.85092005405968 21.545898742072982 19.674602349516583 125-129 23.02689766401332 37.55787940273659 20.69091098277925 18.72431195047084 130-134 24.020194659865716 35.78826835996461 21.136730338833082 19.054806641336594 135-139 25.60818139558907 34.083983265327205 21.068126646350912 19.239708692732812 140-144 24.791301659280634 34.2110687416263 20.998660208183036 19.99896939091003 145-149 26.084014366769036 33.387121961376295 21.128520118682005 19.40034355317266 150-151 26.45169747017958 33.713461790536115 22.047450517761174 17.787390221523136 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 34.0 1 19.0 2 4.0 3 6.0 4 5.5 5 2.5 6 4.0 7 3.5 8 2.0 9 2.5 10 1.0 11 0.0 12 0.5 13 1.5 14 2.0 15 1.0 16 1.0 17 1.0 18 0.5 19 1.5 20 1.5 21 0.5 22 1.0 23 1.5 24 1.0 25 1.0 26 1.5 27 3.0 28 7.0 29 8.5 30 10.0 31 20.0 32 35.5 33 52.5 34 71.5 35 90.5 36 118.0 37 140.5 38 153.0 39 158.0 40 149.5 41 147.0 42 148.5 43 156.5 44 160.5 45 151.0 46 154.5 47 156.0 48 148.0 49 150.0 50 145.5 51 135.5 52 131.5 53 133.5 54 131.5 55 117.0 56 104.0 57 95.5 58 75.0 59 62.0 60 51.5 61 43.5 62 44.5 63 39.5 64 28.0 65 23.5 66 26.0 67 24.0 68 24.5 69 18.0 70 13.0 71 14.0 72 12.5 73 8.5 74 5.0 75 3.0 76 4.5 77 4.5 78 3.0 79 2.0 80 0.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.725 2 1.625 3 1.6 4 1.525 5 1.5 6 1.95 7 1.675 8 1.225 9 1.175 10-14 0.95 15-19 1.685 20-24 2.1350000000000002 25-29 1.8950000000000002 30-34 1.87 35-39 2.085 40-44 1.8900000000000001 45-49 2.3800000000000003 50-54 1.91 55-59 1.9 60-64 2.085 65-69 2.815 70-74 1.955 75-79 1.8950000000000002 80-84 1.9 85-89 1.7149999999999999 90-94 1.975 95-99 2.78 100-104 4.3549999999999995 105-109 4.154999999999999 110-114 4.2 115-119 3.7199999999999998 120-124 3.81 125-129 3.895 130-134 3.9350000000000005 135-139 3.195 140-144 2.97 145-149 3.945 150-151 4.6375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.52499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 95.92850049652432 72.45 2 2.4495200264812977 3.6999999999999997 3 0.7944389275074478 1.7999999999999998 4 0.3972194637537239 1.2 5 0.09930486593843098 0.375 6 0.033101621979477 0.15 7 0.033101621979477 0.17500000000000002 8 0.033101621979477 0.2 9 0.0 0.0 >10 0.19860973187686196 3.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.033101621979477 16.8 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 672 16.8 Illumina Single End PCR Primer 1 (100% over 50bp) AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 34 0.8500000000000001 Illumina Single End PCR Primer 1 (100% over 50bp) AAGCAGAAGACGGCATACGAGATTCGCTTGTGACTGGAGTTCAGACGTGT 25 0.625 Illumina PCR Primer Index 4 (96% over 26bp) NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 25 0.625 No Hit GAGCGGAAGAGCGTCGTGGAGGGAAAGAGGGGAGAGCTCGGTGGTCGCCG 15 0.375 No Hit GATCGGAAGAGCGTCGTGGAGGGAAAGAGGGGAGAGCTCGGTGGTCGCCG 14 0.35000000000000003 Illumina Single End PCR Primer 1 (96% over 29bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCCG 13 0.325 Illumina Single End PCR Primer 1 (98% over 50bp) GAGCGGAAGAGCGCCGTGGAGGGAAAGAGGGGAGAGCTCGGTGGTCGCCG 8 0.2 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 7 0.17500000000000002 No Hit AAGCAGAAGACGGCATACGAGGTTAGCTTGTGACTGGAGTTCAGACGTGT 6 0.15 TruSeq Adapter, Index 10 (96% over 26bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGGAGATCTCGGTGGTCGCCG 5 0.125 Illumina Single End PCR Primer 1 (96% over 31bp) AAGCAGAAGACGGCATACGAGATTCGCTTGGATCGGAAGAGCGTCGTGTA 5 0.125 Illumina PCR Primer Index 4 (96% over 26bp) CGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.975 0.0 0.0 0.0 0.0 2 0.975 0.0 0.0 0.0 0.0 3 0.975 0.0 0.0 0.0 0.0 4 0.975 0.0 0.0 0.0 0.0 5 0.975 0.0 0.0 0.0 0.0 6 0.975 0.0 0.0 0.0 0.0 7 0.975 0.0 0.0 0.0 0.0 8 0.975 0.0 0.0 0.0 0.0 9 0.975 0.0 0.0 0.0 0.0 10-11 0.975 0.0 0.0 0.0 0.0 12-13 0.975 0.0 0.0 0.0 0.0 14-15 1.0 0.0 0.0 0.0 0.0 16-17 1.0 0.0 0.0 0.0 0.0 18-19 1.0 0.0 0.0 0.0 0.0 20-21 1.0 0.0 0.0 0.0 0.0 22-23 1.025 0.0 0.0 0.0 0.0 24-25 1.025 0.0 0.0 0.0 0.0 26-27 1.025 0.0 0.0 0.0 0.0 28-29 1.025 0.0 0.0 0.0 0.0 30-31 1.025 0.0 0.0 0.0 0.0 32-33 1.0875 0.0 0.0 0.0 0.0 34-35 1.1 0.0 0.0 0.0 0.0 36-37 1.15 0.0 0.0 0.0 0.0 38-39 1.2 0.0 0.0 0.0 0.0 40-41 1.225 0.0 0.0 0.0 0.0 42-43 1.25 0.0 0.0 0.0 0.0 44-45 1.2625 0.0 0.0 0.0 0.0 46-47 1.275 0.0 0.0 0.0 0.0 48-49 1.275 0.0 0.0 0.0 0.0 50-51 1.275 0.0 0.0 0.0 0.0 52-53 1.2875 0.0 0.0 0.0 0.0 54-55 1.3 0.0 0.0 0.0 0.0 56-57 1.3625 0.0 0.0 0.0 0.0 58-59 1.4500000000000002 0.0 0.0 0.0 0.0 60-61 1.55 0.0 0.0 0.0 0.0 62-63 1.7125 0.0 0.0 0.0 0.0 64-65 1.775 0.0 0.0 0.0 0.0 66-67 1.7875 0.0 0.0 0.0 0.0 68-69 1.9 0.0 0.0 0.0 0.0 70-71 1.9375 0.0 0.0 0.0 0.0 72-73 2.0125 0.0 0.0 0.0 0.0 74-75 2.025 0.0 0.0 0.0 0.0 76-77 2.0374999999999996 0.0 0.0 0.0 0.0 78-79 2.1125 0.0 0.0 0.0 0.0 80-81 2.2625 0.0 0.0 0.0 0.0 82-83 2.425 0.0 0.0 0.0 0.0 84-85 2.5625 0.0 0.0 0.0 0.0 86-87 2.625 0.0 0.0 0.0 0.0 88-89 2.7750000000000004 0.0 0.0 0.0 0.0 90-91 2.875 0.0 0.0 0.0 0.0 92-93 3.0625 0.0 0.0 0.0 0.0 94-95 3.1875 0.0 0.0 0.0 0.0 96-97 3.4000000000000004 0.0 0.0 0.0 0.0 98-99 3.6125 0.0 0.0 0.0 0.0 100-101 3.8125 0.0 0.0 0.0 0.0 102-103 4.175 0.0 0.0 0.0 0.0 104-105 4.4375 0.0 0.0 0.0 0.0 106-107 4.8125 0.0 0.0 0.0 0.0 108-109 5.0625 0.0 0.0 0.0 0.0 110-111 5.4125 0.0 0.0 0.0 0.0 112-113 5.699999999999999 0.0 0.0 0.0 0.0 114-115 6.15 0.0 0.0 0.0 0.0 116-117 6.6 0.0 0.0 0.0 0.0 118-119 6.925000000000001 0.0 0.0 0.0 0.0 120-121 7.1875 0.0 0.0 0.0 0.0 122-123 7.525 0.0 0.0 0.0 0.0 124-125 7.7125 0.0 0.0 0.0 0.0 126-127 8.025 0.0 0.0 0.0 0.0 128-129 8.575 0.0 0.0 0.0 0.0 130-131 9.087499999999999 0.0 0.0 0.0 0.0 132-133 9.7375 0.0 0.0 0.0 0.0 134-135 10.3375 0.0 0.0 0.0 0.0 136-137 11.024999999999999 0.0 0.0 0.0 0.0 138-139 11.525 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACGG 20 1.8853316E-6 144.71054 7 GAGAGCG 10 0.0068580587 144.71054 6 CGGGAGA 15 1.14563474E-4 144.71053 3 TCGGGAG 15 1.14563474E-4 144.71053 2 GACGGCA 20 2.0156458E-6 142.83118 9 AGACGGC 20 2.0156458E-6 142.83118 8 AGAAGAC 20 3.6018665E-4 108.5329 5 ATCGGGA 20 3.6018665E-4 108.5329 1 GAAGACG 30 1.4163583E-5 96.47368 6 GATCGGA 140 0.0 72.35526 1 GCAGAAG 30 0.0018044587 72.35526 3 CAGAAGA 30 0.0018044587 72.35526 4 AAGCAGA 30 0.0018044587 72.35526 1 CGGAAGA 165 0.0 70.16268 4 AAGAGCG 155 0.0 70.021225 7 TCGGAAG 145 0.0 69.86025 3 ATCGGAA 145 0.0 69.86025 2 GAGCGTC 145 0.0 68.95298 9 AGAGCGT 145 0.0 68.95298 8 GAAGAGC 160 0.0 67.83306 6 >>END_MODULE Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227827 spots for SRR7473323.sra Written 1227827 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra Read 1227816 spots for SRR7473323.sra Written 1227816 spots for SRR7473323.sra SRR ids: ['SRR7473323.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jgubabol SRR7473323.sra spots: 24556331 blocks: [[1, 1227816], [1227817, 2455632], [2455633, 3683448], [3683449, 4911264], [4911265, 6139080], [6139081, 7366896], [7366897, 8594712], [8594713, 9822528], [9822529, 11050344], [11050345, 12278160], [12278161, 13505976], [13505977, 14733792], [14733793, 15961608], [15961609, 17189424], [17189425, 18417240], [18417241, 19645056], [19645057, 20872872], [20872873, 22100688], [22100689, 23328504], [23328505, 24556331]] SRR7473323 file size 8299634 SRR7473323 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473323 SRR7473323_1.fastq SRR7473323_2.fastq Input file: SRR7473323_1.fastq Paired file: SRR7473323_2.fastq trimmed: SRR7473323-trimmed-pair1.fastq, SRR7473323-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 13:20:42 2024 >> started Sat Dec 7 13:21:25 2024 >> done (42.718s) 24556331 read pairs processed; of these: 499212 ( 2.03%) short read pairs filtered out after trimming by size control 6310442 (25.70%) empty read pairs filtered out after trimming by size control 17746677 (72.27%) read pairs available; of these: 12135584 (68.38%) trimmed read pairs available after processing 5611093 (31.62%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 748 0.00% 19 387 0.00% 20 485 0.00% 21 1442 0.01% 22 806 0.00% 23 999 0.01% 24 1193 0.01% 25 867 0.00% 26 901 0.01% 27 893 0.01% 28 1186 0.01% 29 58563 0.33% 30 3385 0.02% 31 8350 0.05% 32 10024 0.06% 33 12843 0.07% 34 10808 0.06% 35 16603 0.09% 36 2792 0.02% 37 6365 0.04% 38 9522 0.05% 39 10679 0.06% 40 18306 0.10% 41 12185 0.07% 42 4451 0.03% 43 2802 0.02% 44 5175 0.03% 45 5522 0.03% 46 7476 0.04% 47 7245 0.04% 48 9956 0.06% 49 10971 0.06% 50 13985 0.08% 51 14617 0.08% 52 20788 0.12% 53 13418 0.08% 54 11609 0.07% 55 23583 0.13% 56 28682 0.16% 57 24922 0.14% 58 36994 0.21% 59 37642 0.21% 60 51262 0.29% 61 49538 0.28% 62 15461 0.09% 63 11108 0.06% 64 13617 0.08% 65 20803 0.12% 66 23855 0.13% 67 39410 0.22% 68 108463 0.61% 69 363718 2.05% 70 404049 2.28% 71 269963 1.52% 72 152809 0.86% 73 84206 0.47% 74 50712 0.29% 75 36310 0.20% 76 29739 0.17% 77 28011 0.16% 78 26563 0.15% 79 26295 0.15% 80 27304 0.15% 81 28884 0.16% 82 30481 0.17% 83 34681 0.20% 84 35264 0.20% 85 31368 0.18% 86 31815 0.18% 87 31351 0.18% 88 32586 0.18% 89 33057 0.19% 90 34864 0.20% 91 35577 0.20% 92 36510 0.21% 93 39669 0.22% 94 40565 0.23% 95 43783 0.25% 96 44207 0.25% 97 44600 0.25% 98 45372 0.26% 99 45898 0.26% 100 46767 0.26% 101 43356 0.24% 102 44375 0.25% 103 45427 0.26% 104 47310 0.27% 105 48579 0.27% 106 47434 0.27% 107 47896 0.27% 108 50372 0.28% 109 53743 0.30% 110 53551 0.30% 111 51943 0.29% 112 53730 0.30% 113 56395 0.32% 114 55943 0.32% 115 60276 0.34% 116 58926 0.33% 117 58136 0.33% 118 58113 0.33% 119 59478 0.34% 120 60991 0.34% 121 61334 0.35% 122 64292 0.36% 123 66862 0.38% 124 67549 0.38% 125 67586 0.38% 126 67955 0.38% 127 70413 0.40% 128 72390 0.41% 129 74767 0.42% 130 77957 0.44% 131 77428 0.44% 132 79480 0.45% 133 81651 0.46% 134 85718 0.48% 135 87537 0.49% 136 90232 0.51% 137 96461 0.54% 138 101465 0.57% 139 105962 0.60% 140 111010 0.63% 141 119759 0.67% 142 130243 0.73% 143 144292 0.81% 144 160808 0.91% 145 187906 1.06% 146 228524 1.29% 147 306060 1.72% 148 462147 2.60% 149 891803 5.03% 150 3789354 21.35% 151 5611093 31.62% 17746677 reads passed initial QC criterion=sequence-density sequence-density=0.60 sequence-density-rank=1 fanout-score=22.85 fanout-score-rank=8 prefix-density=13.34 prefix-fanout=1.0 sequence=TCACAAGCGAAACTCGTATGCCG criterion=fanout-score sequence-density=0.03 sequence-density-rank=27 fanout-score=97.38 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=12.4 sequence=CGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=1.08 sequence-density-rank=1 fanout-score=2.72 fanout-score-rank=27 prefix-density=1.21 prefix-fanout=2.4 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.14 sequence-density-rank=29 fanout-score=60.63 fanout-score-rank=1 prefix-density=8.11 prefix-fanout=1.1 sequence=ATCTCGGTGGTGGCCGTATCATT SRR7473323 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 13:22:59 Started mapping on | Dec 07 13:22:59 Finished on | Dec 07 13:34:59 Mapping speed, Million of reads per hour | 88.73 Number of input reads | 17746677 Average input read length | 255 UNIQUE READS: Uniquely mapped reads number | 9039212 Uniquely mapped reads % | 50.93% Average mapped length | 282.46 Number of splices: Total | 7582611 Number of splices: Annotated (sjdb) | 7076518 Number of splices: GT/AG | 7484186 Number of splices: GC/AG | 85021 Number of splices: AT/AC | 6012 Number of splices: Non-canonical | 7392 Mismatch rate per base, % | 0.18% Deletion rate per base | 0.00% Deletion average length | 1.44 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 91522 % of reads mapped to multiple loci | 0.52% Number of reads mapped to too many loci | 21345 % of reads mapped to too many loci | 0.12% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 47.52% % of reads unmapped: other | 0.90% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8630954 8630954 8630954 N_multimapping 91522 91522 91522 N_noFeature 276762 8744387 365660 N_ambiguous 249961 1561 45318 UnstrandedReadsAssigned:8512489 PositiveStrandReadsAssigned:293264 NegativeStrandReadsAssigned:8628234 Dataset is classified negative stranded MeadianReadLen=142 20thPercentileLength=109 echo kmer=105 SRR7473323 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473323-trimmed-pair1.fastq SRR7473323-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,746,677 reads, 10,600,713 reads pseudoaligned [quant] estimated average fragment length: 217.572 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,120 rounds 52973 SRR7473323.ke.tsv 35125 SRR7473323.se.tsv 88098 total ==> SRR7473323.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 719.71 0 0 PNS24247 1044 827.428 11.3981 1.63612 PNS24249 1928 1711.43 58.1229 4.03368 PNS24246 1044 827.428 11.3981 1.63612 PNS24248 1044 827.428 11.3981 1.63612 PNS24244 1471 1254.43 177.683 16.8233 PNS24243 293 112.68 0 0 KQK14069 1603 1386.43 6954.82 595.801 KQK14071 474 267.005 48.1536 21.4201 ==> SRR7473323.se.tsv <== BRADI_1g14170v3 5717 BRADI_1g53295v3 21 BRADI_1g59795v3 271 BRADI_1g07683v3 0 BRADI_1g00485v3 50 BRADI_1g20270v3 556 BRADI_1g74790v3 25 BRADI_1g09890v3 2 BRADI_1g77505v3 227 BRADI_1g48960v3 0 SRR7473323 completed mapping pipeline successfully