Starting /dee2/code/volunteer_pipeline.sh SRR7473324 current disk space = 1543171289088 free memory = 1596475440 SRR7473324 SRAfilesize 4f915e1a9b4d72a69c66dc3c1fbc1d5a SRR7473324.sra SRR7473324.sra file validated SRR7473324 is paired end SRR7473324 is conventional basespace SRR7473324 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473324_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.52475 34.0 34.0 34.0 33.0 34.0 2 33.5255 34.0 34.0 34.0 33.0 34.0 3 33.56525 34.0 34.0 34.0 33.0 34.0 4 33.53775 34.0 34.0 34.0 33.0 34.0 5 33.56575 34.0 34.0 34.0 33.0 34.0 6 37.3415 38.0 38.0 38.0 37.0 38.0 7 37.5495 38.0 38.0 38.0 37.0 38.0 8 37.53525 38.0 38.0 38.0 38.0 38.0 9 37.62575 38.0 38.0 38.0 38.0 38.0 10-14 37.529250000000005 38.0 38.0 38.0 38.0 38.0 15-19 37.587149999999994 38.0 38.0 38.0 38.0 38.0 20-24 37.60355 38.0 38.0 38.0 38.0 38.0 25-29 37.51255 38.0 38.0 38.0 38.0 38.0 30-34 37.372400000000006 38.0 38.0 38.0 37.4 38.0 35-39 37.484750000000005 38.0 38.0 38.0 38.0 38.0 40-44 37.142849999999996 38.0 38.0 38.0 36.8 38.0 45-49 37.27615 38.0 38.0 38.0 37.2 38.0 50-54 37.1348 38.0 38.0 38.0 36.8 38.0 55-59 37.08515 38.0 38.0 38.0 36.2 38.0 60-64 37.2491 38.0 38.0 38.0 37.0 38.0 65-69 37.05565 38.0 38.0 38.0 36.4 38.0 70-74 37.13835 38.0 38.0 38.0 37.0 38.0 75-79 36.88315 38.0 38.0 38.0 36.4 38.0 80-84 36.747600000000006 38.0 38.0 38.0 36.2 38.0 85-89 36.8057 38.0 38.0 38.0 36.0 38.0 90-94 36.703599999999994 38.0 38.0 38.0 36.0 38.0 95-99 36.672650000000004 38.0 38.0 38.0 35.6 38.0 100-104 36.59585 38.0 38.0 38.0 35.2 38.0 105-109 36.349000000000004 38.0 38.0 38.0 34.6 38.0 110-114 36.30985 38.0 38.0 38.0 34.2 38.0 115-119 36.20395 38.0 38.0 38.0 34.0 38.0 120-124 36.033550000000005 38.0 38.0 38.0 33.6 38.0 125-129 35.7143 38.0 38.0 38.0 33.0 38.0 130-134 35.5022 38.0 37.6 38.0 32.2 38.0 135-139 35.313550000000006 38.0 36.0 38.0 31.0 38.0 140-144 35.057750000000006 38.0 36.0 38.0 30.4 38.0 145-149 34.375099999999996 38.0 35.6 38.0 26.6 38.0 150-151 31.14375 36.5 31.0 38.0 12.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 1.0 9 2.0 10 0.0 11 1.0 12 2.0 13 1.0 14 5.0 15 8.0 16 3.0 17 5.0 18 11.0 19 15.0 20 4.0 21 4.0 22 5.0 23 6.0 24 4.0 25 16.0 26 7.0 27 14.0 28 18.0 29 29.0 30 27.0 31 40.0 32 57.0 33 78.0 34 85.0 35 176.0 36 439.0 37 2936.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.275000000000006 15.1 10.299999999999999 31.324999999999996 2 24.768576432324245 15.511633725293972 31.298473855391546 28.42131598699024 3 20.674999999999997 22.15 26.8 30.375000000000004 4 23.25 28.125 23.275000000000002 25.35 5 23.325000000000003 32.375 23.875 20.424999999999997 6 19.925 32.125 25.8 22.15 7 16.625 21.425 40.849999999999994 21.099999999999998 8 18.05 21.825 30.125 30.0 9 18.65 22.25 31.0 28.1 10-14 20.935000000000002 26.22 24.705 28.139999999999997 15-19 21.17 25.71 26.419999999999998 26.700000000000003 20-24 20.965 26.284999999999997 25.645 27.105 25-29 20.895 25.755 26.064999999999998 27.284999999999997 30-34 21.59 25.8 26.009999999999998 26.6 35-39 21.060000000000002 26.365 25.665 26.91 40-44 21.705 25.835 25.624999999999996 26.834999999999997 45-49 21.755 25.8 25.779999999999998 26.665 50-54 21.745 25.895000000000003 25.840000000000003 26.52 55-59 21.83 25.245 26.029999999999998 26.895000000000003 60-64 21.6 24.745 26.76 26.895000000000003 65-69 21.25 25.195 25.929999999999996 27.625 70-74 21.5 26.815 24.795 26.889999999999997 75-79 21.66 26.25 24.87 27.22 80-84 21.435000000000002 26.43 25.535000000000004 26.6 85-89 21.84 25.495 25.080000000000002 27.584999999999997 90-94 22.05 24.92 25.629999999999995 27.400000000000002 95-99 22.235 25.759999999999998 25.119999999999997 26.884999999999998 100-104 22.485 26.545 24.55 26.419999999999998 105-109 22.245 25.89 24.065 27.800000000000004 110-114 22.577257725772576 25.38753875387539 24.412441244124413 27.622762276227625 115-119 22.5 24.965 24.975 27.560000000000002 120-124 22.475 25.929999999999996 23.880000000000003 27.715 125-129 22.975 25.39 25.31 26.325 130-134 21.92 26.224999999999998 25.03 26.825 135-139 22.38 26.375 24.05 27.195000000000004 140-144 23.14 26.119999999999997 23.72 27.02 145-149 22.520899033888973 26.07999199078941 24.257896581068227 27.14121239425339 150-151 22.3625 25.5 24.337500000000002 27.800000000000004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.5 2 2.5 3 1.5 4 0.5 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.5 23 1.0 24 1.5 25 1.5 26 2.5 27 5.0 28 4.0 29 4.5 30 6.5 31 7.0 32 17.0 33 24.5 34 31.0 35 49.5 36 63.0 37 81.0 38 103.0 39 107.0 40 117.0 41 141.5 42 152.0 43 157.0 44 171.5 45 163.0 46 145.0 47 143.0 48 131.0 49 134.0 50 155.0 51 162.0 52 169.5 53 204.5 54 233.0 55 232.5 56 175.5 57 121.5 58 117.0 59 100.0 60 80.0 61 57.5 62 43.5 63 34.5 64 25.5 65 20.5 66 15.0 67 12.0 68 11.0 69 10.5 70 8.5 71 10.0 72 8.0 73 4.5 74 4.5 75 3.0 76 2.0 77 1.5 78 1.5 79 1.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.01 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.11499999999999999 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.5 #Duplication Level Percentage of deduplicated Percentage of total 1 92.97687861271676 80.425 2 4.017341040462428 6.950000000000001 3 1.1271676300578035 2.9250000000000003 4 0.5491329479768786 1.9 5 0.6069364161849711 2.625 6 0.26011560693641617 1.35 7 0.20231213872832368 1.225 8 0.028901734104046246 0.2 9 0.028901734104046246 0.22499999999999998 >10 0.20231213872832368 2.175 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC 20 0.5 TruSeq Adapter, Index 4 (100% over 50bp) GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 16 0.4 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 11 0.27499999999999997 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 10 0.25 No Hit GTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAGCC 10 0.25 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 10 0.25 No Hit GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGA 10 0.25 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 9 0.22499999999999998 No Hit AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA 8 0.2 No Hit GTTTGATTGGCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAA 7 0.17500000000000002 No Hit GGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGAC 7 0.17500000000000002 No Hit GCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACAC 7 0.17500000000000002 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 7 0.17500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 7 0.17500000000000002 No Hit GGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTAC 7 0.17500000000000002 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 7 0.17500000000000002 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG 6 0.15 No Hit GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTA 6 0.15 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 6 0.15 No Hit GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT 6 0.15 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 6 0.15 No Hit CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC 6 0.15 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 6 0.15 No Hit GGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGA 6 0.15 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC 6 0.15 No Hit CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT 5 0.125 No Hit GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCT 5 0.125 No Hit CCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTAC 5 0.125 No Hit GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT 5 0.125 No Hit GCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCCACATCGTTTC 5 0.125 No Hit GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG 5 0.125 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 5 0.125 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 5 0.125 No Hit GTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATC 5 0.125 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 5 0.125 No Hit CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT 5 0.125 No Hit GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC 5 0.125 No Hit GCCGTACTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACGC 5 0.125 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 5 0.125 No Hit ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC 5 0.125 No Hit GGGAGAACCAGCTATCTCCCGGTTTGATTGGCCTTTCACCCCCAGCCACA 5 0.125 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 5 0.125 No Hit GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC 5 0.125 No Hit GTCGGTTTGGGGTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCT 5 0.125 No Hit GCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTA 5 0.125 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.07500000000000001 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.325 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.7124999999999999 0.0 0.0 0.0 0.0 86-87 0.9 0.0 0.0 0.0 0.0 88-89 1.1 0.0 0.0 0.0 0.0 90-91 1.2000000000000002 0.0 0.0 0.0 0.0 92-93 1.4375 0.0 0.0 0.0 0.0 94-95 1.7374999999999998 0.0 0.0 0.0 0.0 96-97 2.1624999999999996 0.0 0.0 0.0 0.0 98-99 2.5374999999999996 0.0 0.0 0.0 0.0 100-101 2.9625 0.0 0.0 0.0 0.0 102-103 3.4875 0.0 0.0 0.0 0.0 104-105 3.8625 0.0 0.0 0.0 0.0 106-107 4.4 0.0 0.0 0.0 0.0 108-109 4.875 0.0 0.0 0.0 0.0 110-111 5.3125 0.0 0.0 0.0 0.0 112-113 5.887499999999999 0.0 0.0 0.0 0.0 114-115 6.6 0.0 0.0 0.0 0.0 116-117 7.375 0.0 0.0 0.0 0.0 118-119 7.949999999999999 0.0 0.0 0.0 0.0 120-121 8.537500000000001 0.0 0.0 0.0 0.0 122-123 9.025 0.0 0.0 0.0 0.0 124-125 9.7 0.0 0.0 0.0 0.0 126-127 10.3875 0.0 0.0 0.0 0.0 128-129 11.1 0.0 0.0 0.0 0.0 130-131 11.7375 0.0 0.0 0.0 0.0 132-133 12.3375 0.0 0.0 0.0 0.0 134-135 13.6625 0.0 0.0 0.0 0.0 136-137 14.7875 0.0 0.0 0.0 0.0 138-139 15.5375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGGGA 10 0.006830828 145.0 9 GTACAAG 10 0.006830828 145.0 1 GGCCCGG 10 0.006830828 145.0 7 AAGGCCC 10 0.006830828 145.0 5 ACAAGGC 10 0.006830828 145.0 3 CAAGGCC 10 0.006830828 145.0 4 TTGTATG 20 3.5877043E-4 108.75 145 AAAAAAA 50 5.60876E-5 20.3 75-79 >>END_MODULE SRR7473324 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473324_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.11125 34.0 33.0 34.0 32.0 34.0 2 33.15675 34.0 33.0 34.0 33.0 34.0 3 33.03375 34.0 33.0 34.0 33.0 34.0 4 33.043 34.0 33.0 34.0 33.0 34.0 5 33.043 34.0 33.0 34.0 33.0 34.0 6 37.04875 38.0 38.0 38.0 37.0 38.0 7 36.9085 38.0 38.0 38.0 37.0 38.0 8 36.98175 38.0 38.0 38.0 37.0 38.0 9 37.07675 38.0 38.0 38.0 37.0 38.0 10-14 37.042899999999996 38.0 38.0 38.0 37.2 38.0 15-19 37.080600000000004 38.0 38.0 38.0 37.6 38.0 20-24 37.08425 38.0 38.0 38.0 37.6 38.0 25-29 37.10385 38.0 38.0 38.0 37.6 38.0 30-34 37.147000000000006 38.0 38.0 38.0 38.0 38.0 35-39 36.96635 38.0 38.0 38.0 37.2 38.0 40-44 36.960449999999994 38.0 38.0 38.0 37.2 38.0 45-49 37.0114 38.0 38.0 38.0 37.0 38.0 50-54 37.01845 38.0 38.0 38.0 37.0 38.0 55-59 37.02695 38.0 38.0 38.0 37.0 38.0 60-64 37.032599999999995 38.0 38.0 38.0 37.0 38.0 65-69 36.7911 38.0 38.0 38.0 36.6 38.0 70-74 36.68025 38.0 38.0 38.0 36.6 38.0 75-79 36.68005 38.0 38.0 38.0 36.8 38.0 80-84 36.5768 38.0 38.0 38.0 36.0 38.0 85-89 36.521550000000005 38.0 38.0 38.0 36.0 38.0 90-94 36.36285 38.0 38.0 38.0 35.0 38.0 95-99 36.3798 38.0 38.0 38.0 35.0 38.0 100-104 36.25605 38.0 38.0 38.0 35.0 38.0 105-109 36.056650000000005 38.0 38.0 38.0 34.4 38.0 110-114 35.826350000000005 38.0 38.0 38.0 34.0 38.0 115-119 35.32085 38.0 37.4 38.0 30.2 38.0 120-124 35.560500000000005 38.0 38.0 38.0 33.0 38.0 125-129 35.43085 38.0 37.6 38.0 32.2 38.0 130-134 34.893800000000006 38.0 36.0 38.0 29.2 38.0 135-139 34.63715 38.0 36.0 38.0 27.6 38.0 140-144 34.0101 38.0 34.8 38.0 24.6 38.0 145-149 33.36795 38.0 33.4 38.0 18.6 38.0 150-151 29.042625 35.5 24.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 3.0 4 4.0 5 0.0 6 3.0 7 6.0 8 1.0 9 2.0 10 2.0 11 8.0 12 7.0 13 2.0 14 1.0 15 8.0 16 2.0 17 23.0 18 7.0 19 8.0 20 11.0 21 2.0 22 8.0 23 8.0 24 7.0 25 19.0 26 14.0 27 17.0 28 19.0 29 37.0 30 30.0 31 31.0 32 45.0 33 74.0 34 109.0 35 182.0 36 469.0 37 2820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.725 18.5 10.8 22.975 2 31.74881160870653 21.641230923192396 25.794345759319487 20.815611708781585 3 26.207759699624532 24.330413016270338 27.80976220275344 21.65206508135169 4 29.236545682102626 31.23904881101377 19.799749687108886 19.72465581977472 5 29.429429429429426 33.408408408408405 18.593593593593592 18.56856856856857 6 26.025 34.725 18.025 21.224999999999998 7 22.650000000000002 19.7 33.725 23.925 8 25.656414103525883 23.10577644411103 21.50537634408602 29.732433108277068 9 25.7 23.275000000000002 23.974999999999998 27.05 10-14 28.30132052821128 25.350140056022408 21.97378951580632 24.374749899959983 15-19 28.118435530659198 24.75742722816845 23.562068620586178 23.562068620586178 20-24 28.422842284228423 25.06250625062506 23.587358735873586 22.927292729272928 25-29 27.673301990597178 26.472941882564772 23.592077623286986 22.261678503551067 30-34 28.451422571128553 25.93629681484074 23.396169808490423 22.216110805540275 35-39 27.47186796699175 25.45136284071018 24.241060265066267 22.835708927231806 40-44 27.754163124468672 25.978896834525177 24.4436665499825 21.823273491023652 45-49 27.70692673168292 25.751437859464865 24.47611902975744 22.065516379094774 50-54 27.67691922980745 25.561390347586897 24.406101525381345 22.355588897224308 55-59 27.730546109221844 25.140028005601124 25.250050010002 21.879375875175036 60-64 27.297283777699967 26.076734530538744 24.711120004001803 21.91486168775949 65-69 28.117493995196156 26.090872698158527 24.419535628502803 21.372097678142516 70-74 27.738321496448936 26.57297189156747 23.94218265479644 21.746523957187154 75-79 27.444116617492625 25.748862329349404 24.393659048857327 22.413362004300645 80-84 27.68192048012003 26.076519129782444 24.976244061015255 21.26531632908227 85-89 27.97 25.94 24.355 21.735 90-94 27.38821646493948 25.782734820446134 25.10253075922777 21.726517955386615 95-99 27.2013600680034 26.82134106705335 24.501225061253063 21.476073803690184 100-104 27.884999999999998 26.155 24.64 21.32 105-109 26.763028908672602 26.868060418125438 24.147244173251973 22.221666499949986 110-114 28.2689227072487 26.426712054465355 24.078894673608332 21.22547056467761 115-119 28.11795924498072 26.520803084163617 24.543133229860313 20.818104440995345 120-124 28.109054527263634 26.718359179589797 23.871935967983994 21.300650325162582 125-129 28.205000000000002 26.865 24.169999999999998 20.76 130-134 27.765 26.935 23.65 21.65 135-139 28.7 26.755000000000003 23.835 20.71 140-144 29.244999999999997 26.919999999999998 23.125 20.71 145-149 29.185 26.57 23.775 20.47 150-151 29.012500000000003 26.424999999999997 23.45 21.1125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.5 16 1.0 17 1.0 18 0.5 19 0.5 20 0.5 21 0.5 22 0.5 23 0.0 24 0.0 25 0.5 26 1.0 27 1.0 28 2.0 29 4.5 30 5.5 31 8.5 32 10.0 33 13.0 34 23.0 35 25.5 36 32.0 37 43.5 38 61.0 39 86.5 40 100.0 41 115.5 42 129.0 43 129.5 44 130.0 45 142.0 46 151.0 47 149.0 48 161.0 49 176.0 50 181.0 51 166.0 52 198.5 53 249.5 54 247.5 55 225.0 56 190.0 57 149.5 58 114.5 59 91.5 60 72.0 61 68.5 62 65.0 63 44.0 64 37.5 65 33.0 66 20.0 67 23.5 68 26.0 69 24.0 70 17.5 71 13.5 72 11.5 73 7.0 74 5.5 75 3.0 76 2.0 77 2.5 78 2.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.125 4 0.125 5 0.1 6 0.0 7 0.0 8 0.025 9 0.0 10-14 0.04 15-19 0.03 20-24 0.01 25-29 0.03 30-34 0.005 35-39 0.025 40-44 0.015 45-49 0.025 50-54 0.025 55-59 0.02 60-64 0.045 65-69 0.08 70-74 0.03 75-79 0.015 80-84 0.025 85-89 0.0 90-94 0.03 95-99 0.005 100-104 0.0 105-109 0.03 110-114 0.12 115-119 0.135 120-124 0.05 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.05000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 93.36588167719701 81.27499999999999 2 3.963239517518667 6.9 3 1.2923607122343481 3.375 4 0.43078690407811604 1.5 5 0.2871912693854107 1.25 6 0.14359563469270534 0.75 7 0.14359563469270534 0.8750000000000001 8 0.14359563469270534 1.0 9 0.0 0.0 >10 0.22975301550832855 3.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 25 0.625 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 20 0.5 Illumina Single End PCR Primer 1 (100% over 50bp) GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 15 0.375 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 14 0.35000000000000003 No Hit CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 13 0.325 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 13 0.325 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 13 0.325 No Hit ACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA 10 0.25 No Hit GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTG 8 0.2 No Hit CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT 8 0.2 No Hit GTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTT 8 0.2 No Hit GGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATG 8 0.2 No Hit GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC 8 0.2 No Hit GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA 7 0.17500000000000002 No Hit GGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATG 7 0.17500000000000002 No Hit GCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAAC 7 0.17500000000000002 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 7 0.17500000000000002 No Hit AGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAAC 7 0.17500000000000002 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 6 0.15 No Hit GTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA 6 0.15 No Hit GGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGA 6 0.15 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 6 0.15 No Hit AGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTG 6 0.15 No Hit AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGG 5 0.125 No Hit CCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT 5 0.125 No Hit GTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGAC 5 0.125 No Hit GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGAT 5 0.125 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 5 0.125 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 5 0.125 No Hit CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAG 5 0.125 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC 5 0.125 No Hit GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGC 5 0.125 No Hit CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.325 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.7 0.0 0.0 0.0 0.0 86-87 0.875 0.0 0.0 0.0 0.0 88-89 1.1 0.0 0.0 0.0 0.0 90-91 1.2000000000000002 0.0 0.0 0.0 0.0 92-93 1.4 0.0 0.0 0.0 0.0 94-95 1.725 0.0 0.0 0.0 0.0 96-97 2.175 0.0 0.0 0.0 0.0 98-99 2.5625 0.0 0.0 0.0 0.0 100-101 2.975 0.0 0.0 0.0 0.0 102-103 3.5250000000000004 0.0 0.0 0.0 0.0 104-105 3.9375 0.0 0.0 0.0 0.0 106-107 4.475 0.0 0.0 0.0 0.0 108-109 4.925 0.0 0.0 0.0 0.0 110-111 5.375 0.0 0.0 0.0 0.0 112-113 5.9375 0.0 0.0 0.0 0.0 114-115 6.6375 0.0 0.0 0.0 0.0 116-117 7.3375 0.0 0.0 0.0 0.0 118-119 7.85 0.0 0.0 0.0 0.0 120-121 8.3875 0.0 0.0 0.0 0.0 122-123 8.9 0.0 0.0 0.0 0.0 124-125 9.625 0.0 0.0 0.0 0.0 126-127 10.3625 0.0 0.0 0.0 0.0 128-129 11.05 0.0 0.0 0.0 0.0 130-131 11.6625 0.0 0.0 0.0 0.0 132-133 12.25 0.0 0.0 0.0 0.0 134-135 13.525 0.0 0.0 0.0 0.0 136-137 14.5875 0.0 0.0 0.0 0.0 138-139 15.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACGCT 10 0.006830828 145.0 9 TCAGATT 10 0.006830828 145.0 2 CAGATTG 10 0.006830828 145.0 3 TTGAACG 10 0.006830828 145.0 7 TGAACGC 10 0.006830828 145.0 8 AGATTGA 10 0.006830828 145.0 4 >>END_MODULE Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881938 spots for SRR7473324.sra Written 881938 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra Read 881928 spots for SRR7473324.sra Written 881928 spots for SRR7473324.sra SRR ids: ['SRR7473324.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_i1u8c9oh SRR7473324.sra spots: 17638570 blocks: [[1, 881928], [881929, 1763856], [1763857, 2645784], [2645785, 3527712], [3527713, 4409640], [4409641, 5291568], [5291569, 6173496], [6173497, 7055424], [7055425, 7937352], [7937353, 8819280], [8819281, 9701208], [9701209, 10583136], [10583137, 11465064], [11465065, 12346992], [12346993, 13228920], [13228921, 14110848], [14110849, 14992776], [14992777, 15874704], [15874705, 16756632], [16756633, 17638570]] SRR7473324 file size 5955432 SRR7473324 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473324 SRR7473324_1.fastq SRR7473324_2.fastq Input file: SRR7473324_1.fastq Paired file: SRR7473324_2.fastq trimmed: SRR7473324-trimmed-pair1.fastq, SRR7473324-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 13:21:20 2024 >> started Sat Dec 7 13:21:42 2024 >> done (22.570s) 17638570 read pairs processed; of these: 46816 ( 0.27%) short read pairs filtered out after trimming by size control 152260 ( 0.86%) empty read pairs filtered out after trimming by size control 17439494 (98.87%) read pairs available; of these: 8635924 (49.52%) trimmed read pairs available after processing 8803570 (50.48%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 26 0.00% 20 18 0.00% 21 17 0.00% 22 27 0.00% 23 29 0.00% 24 39 0.00% 25 30 0.00% 26 26 0.00% 27 36 0.00% 28 34 0.00% 29 37 0.00% 30 54 0.00% 31 45 0.00% 32 41 0.00% 33 62 0.00% 34 63 0.00% 35 63 0.00% 36 84 0.00% 37 68 0.00% 38 87 0.00% 39 99 0.00% 40 121 0.00% 41 120 0.00% 42 140 0.00% 43 134 0.00% 44 183 0.00% 45 184 0.00% 46 226 0.00% 47 271 0.00% 48 321 0.00% 49 318 0.00% 50 380 0.00% 51 389 0.00% 52 457 0.00% 53 484 0.00% 54 514 0.00% 55 546 0.00% 56 632 0.00% 57 639 0.00% 58 717 0.00% 59 863 0.00% 60 1058 0.01% 61 1281 0.01% 62 1411 0.01% 63 1584 0.01% 64 1751 0.01% 65 2270 0.01% 66 2218 0.01% 67 2709 0.02% 68 4236 0.02% 69 17182 0.10% 70 20940 0.12% 71 11202 0.06% 72 7426 0.04% 73 6713 0.04% 74 6199 0.04% 75 6215 0.04% 76 6095 0.03% 77 6419 0.04% 78 6912 0.04% 79 7976 0.05% 80 8941 0.05% 81 9946 0.06% 82 11893 0.07% 83 14186 0.08% 84 18093 0.10% 85 19102 0.11% 86 19679 0.11% 87 19791 0.11% 88 20981 0.12% 89 21336 0.12% 90 23153 0.13% 91 26997 0.15% 92 25850 0.15% 93 32213 0.18% 94 33551 0.19% 95 35798 0.21% 96 34958 0.20% 97 33244 0.19% 98 32611 0.19% 99 33207 0.19% 100 38248 0.22% 101 36434 0.21% 102 39795 0.23% 103 42277 0.24% 104 46411 0.27% 105 51418 0.29% 106 47601 0.27% 107 45615 0.26% 108 48115 0.28% 109 58454 0.34% 110 57509 0.33% 111 48880 0.28% 112 52934 0.30% 113 64902 0.37% 114 59787 0.34% 115 64313 0.37% 116 64374 0.37% 117 59086 0.34% 118 61082 0.35% 119 59106 0.34% 120 62302 0.36% 121 60263 0.35% 122 64206 0.37% 123 68429 0.39% 124 74669 0.43% 125 74915 0.43% 126 73582 0.42% 127 74569 0.43% 128 72510 0.42% 129 73070 0.42% 130 74284 0.43% 131 74689 0.43% 132 77819 0.45% 133 83986 0.48% 134 89004 0.51% 135 97046 0.56% 136 97652 0.56% 137 101808 0.58% 138 100549 0.58% 139 99170 0.57% 140 99714 0.57% 141 112198 0.64% 142 111836 0.64% 143 121026 0.69% 144 134117 0.77% 145 152055 0.87% 146 178675 1.02% 147 221186 1.27% 148 308517 1.77% 149 580555 3.33% 150 3369221 19.32% 151 8803570 50.48% 17439494 reads passed initial QC criterion=sequence-density sequence-density=0.64 sequence-density-rank=1 fanout-score=1.76 fanout-score-rank=39 prefix-density=1.10 prefix-fanout=1.0 sequence=TAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=21.36 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=3.8 sequence=GCAAAGAACGCTTCTTTAAGGTAAGGAGGTGATCCAACCGCAGGTTCCCCTACGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGC criterion=sequence-density sequence-density=2.86 sequence-density-rank=1 fanout-score=2.08 fanout-score-rank=34 prefix-density=2.93 prefix-fanout=2.0 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.08 sequence-density-rank=36 fanout-score=43.71 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=12.8 sequence=GTGGAGAAGAAGGACCCAACCGGCGCCAAGGTCACCAAGGC SRR7473324 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 13:22:57 Started mapping on | Dec 07 13:22:58 Finished on | Dec 07 13:37:36 Mapping speed, Million of reads per hour | 71.51 Number of input reads | 17439494 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 11276484 Uniquely mapped reads % | 64.66% Average mapped length | 290.16 Number of splices: Total | 9277410 Number of splices: Annotated (sjdb) | 8729428 Number of splices: GT/AG | 9152835 Number of splices: GC/AG | 108275 Number of splices: AT/AC | 6287 Number of splices: Non-canonical | 10013 Mismatch rate per base, % | 0.13% Deletion rate per base | 0.01% Deletion average length | 1.47 Insertion rate per base | 0.00% Insertion average length | 1.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 176011 % of reads mapped to multiple loci | 1.01% Number of reads mapped to too many loci | 28886 % of reads mapped to too many loci | 0.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 31.43% % of reads unmapped: other | 2.73% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6010619 6010619 6010619 N_multimapping 176011 176011 176011 N_noFeature 299589 10887120 416783 N_ambiguous 301520 1315 30205 UnstrandedReadsAssigned:10675375 PositiveStrandReadsAssigned:388049 NegativeStrandReadsAssigned:10829496 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=144 echo kmer=139 SRR7473324 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473324-trimmed-pair1.fastq SRR7473324-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,439,494 reads, 11,047,817 reads pseudoaligned [quant] estimated average fragment length: 236.459 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,078 rounds 52973 SRR7473324.ke.tsv 35125 SRR7473324.se.tsv 88098 total ==> SRR7473324.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 700.848 0 0 PNS24247 1044 808.541 9.98336 1.44118 PNS24249 1928 1692.54 10.2226 0.704962 PNS24246 1044 808.541 9.98336 1.44118 PNS24248 1044 808.541 9.98336 1.44118 PNS24244 1471 1235.54 217.827 20.5777 PNS24243 293 104.937 0 0 KQK14069 1603 1367.54 210.241 17.944 KQK14071 474 253.608 1.93581 0.890929 ==> SRR7473324.se.tsv <== BRADI_1g14170v3 229 BRADI_1g53295v3 10 BRADI_1g59795v3 147 BRADI_1g07683v3 0 BRADI_1g00485v3 30 BRADI_1g20270v3 920 BRADI_1g74790v3 51 BRADI_1g09890v3 0 BRADI_1g77505v3 228 BRADI_1g48960v3 1 SRR7473324 completed mapping pipeline successfully