Starting /dee2/code/volunteer_pipeline.sh SRR7473327 current disk space = 1543094296576 free memory = 1590216636 SRR7473327 SRAfilesize e893fe7091d77a76dde5fa2e0ee7bf06 SRR7473327.sra SRR7473327.sra file validated SRR7473327 is paired end SRR7473327 is conventional basespace SRR7473327 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473327_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.5155 34.0 34.0 34.0 33.0 34.0 2 33.51575 34.0 34.0 34.0 33.0 34.0 3 33.545 34.0 34.0 34.0 33.0 34.0 4 33.5265 34.0 34.0 34.0 33.0 34.0 5 33.5635 34.0 34.0 34.0 33.0 34.0 6 37.354 38.0 38.0 38.0 37.0 38.0 7 37.56625 38.0 38.0 38.0 38.0 38.0 8 37.5095 38.0 38.0 38.0 38.0 38.0 9 37.53675 38.0 38.0 38.0 38.0 38.0 10-14 37.530150000000006 38.0 38.0 38.0 38.0 38.0 15-19 37.6229 38.0 38.0 38.0 38.0 38.0 20-24 37.600199999999994 38.0 38.0 38.0 38.0 38.0 25-29 37.5191 38.0 38.0 38.0 38.0 38.0 30-34 37.39195 38.0 38.0 38.0 37.4 38.0 35-39 37.549800000000005 38.0 38.0 38.0 38.0 38.0 40-44 37.245000000000005 38.0 38.0 38.0 37.2 38.0 45-49 37.3232 38.0 38.0 38.0 37.4 38.0 50-54 37.205850000000005 38.0 38.0 38.0 37.0 38.0 55-59 37.13505 38.0 38.0 38.0 36.6 38.0 60-64 37.265699999999995 38.0 38.0 38.0 37.0 38.0 65-69 37.0958 38.0 38.0 38.0 36.6 38.0 70-74 37.22135 38.0 38.0 38.0 37.0 38.0 75-79 37.0558 38.0 38.0 38.0 37.0 38.0 80-84 36.861850000000004 38.0 38.0 38.0 36.2 38.0 85-89 36.9367 38.0 38.0 38.0 36.6 38.0 90-94 36.798950000000005 38.0 38.0 38.0 35.8 38.0 95-99 36.75775 38.0 38.0 38.0 36.0 38.0 100-104 36.66685 38.0 38.0 38.0 35.2 38.0 105-109 36.5114 38.0 38.0 38.0 34.8 38.0 110-114 36.45395 38.0 38.0 38.0 34.8 38.0 115-119 36.38385 38.0 38.0 38.0 34.2 38.0 120-124 36.17985 38.0 38.0 38.0 33.8 38.0 125-129 35.9838 38.0 38.0 38.0 33.4 38.0 130-134 35.706 38.0 37.4 38.0 32.6 38.0 135-139 35.46750000000001 38.0 36.4 38.0 31.6 38.0 140-144 35.31185000000001 38.0 36.0 38.0 31.2 38.0 145-149 34.67620000000001 38.0 35.6 38.0 28.8 38.0 150-151 31.336 36.5 31.0 38.0 14.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 1.0 9 0.0 10 0.0 11 1.0 12 0.0 13 0.0 14 4.0 15 3.0 16 7.0 17 2.0 18 5.0 19 17.0 20 5.0 21 4.0 22 5.0 23 5.0 24 6.0 25 11.0 26 20.0 27 10.0 28 19.0 29 21.0 30 28.0 31 44.0 32 42.0 33 55.0 34 83.0 35 173.0 36 462.0 37 2965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.625 10.725 11.924999999999999 38.725 2 23.936968484242122 15.507753876938468 30.71535767883942 29.839919959979987 3 23.0 19.0 24.425 33.575 4 24.775 27.250000000000004 22.275 25.7 5 25.424999999999997 29.425 24.625 20.525 6 21.675 30.2 26.025 22.1 7 15.475 21.9 42.475 20.150000000000002 8 19.25 22.75 29.875 28.125 9 21.25 21.725 32.225 24.8 10-14 22.03 26.02 25.95 26.0 15-19 22.040000000000003 24.834999999999997 27.255000000000003 25.869999999999997 20-24 22.14 25.45 26.529999999999998 25.88 25-29 21.485000000000003 25.590000000000003 27.205000000000002 25.72 30-34 21.235 25.755 26.355 26.655 35-39 21.33 24.94 28.365000000000002 25.365 40-44 22.095000000000002 25.09 26.950000000000003 25.865 45-49 22.07 25.485000000000003 26.77 25.674999999999997 50-54 22.439999999999998 26.235000000000003 25.419999999999998 25.905 55-59 21.935 24.965 27.115000000000002 25.985000000000003 60-64 21.54 25.255 27.66 25.545 65-69 21.495 25.75 27.389999999999997 25.365 70-74 21.39 26.215 26.16 26.235000000000003 75-79 21.52 26.07 25.869999999999997 26.540000000000003 80-84 21.925 25.955000000000002 26.02 26.1 85-89 22.285 25.759999999999998 26.064999999999998 25.89 90-94 22.53 25.245 25.929999999999996 26.295 95-99 22.48 25.619999999999997 26.38 25.52 100-104 22.25 26.1 26.305 25.345000000000002 105-109 22.31 25.255 26.045 26.39 110-114 22.16610830541527 26.01130056502825 25.526276313815693 26.296314815740786 115-119 21.61 26.515 25.72 26.155 120-124 22.0 25.88 24.959999999999997 27.16 125-129 22.415 25.615 26.445 25.525 130-134 22.845 26.345000000000002 25.124999999999996 25.685000000000002 135-139 22.015 26.44 26.375 25.169999999999998 140-144 22.93 26.43 25.06 25.580000000000002 145-149 23.078462770216174 26.21597277822258 25.41533226581265 25.2902321857486 150-151 22.3625 25.25 26.474999999999998 25.912499999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 0.5 22 2.0 23 1.5 24 0.5 25 0.5 26 2.0 27 3.5 28 5.0 29 13.5 30 18.0 31 22.5 32 36.0 33 52.5 34 66.0 35 73.0 36 84.5 37 114.5 38 126.5 39 110.0 40 113.5 41 108.5 42 105.0 43 126.5 44 144.5 45 149.0 46 145.0 47 142.0 48 142.0 49 144.5 50 154.0 51 157.5 52 183.5 53 213.5 54 210.5 55 190.5 56 133.5 57 104.0 58 105.0 59 91.5 60 71.5 61 61.5 62 54.5 63 38.5 64 29.5 65 20.0 66 17.0 67 19.5 68 18.0 69 16.0 70 12.5 71 10.0 72 7.0 73 4.5 74 4.5 75 4.5 76 3.0 77 2.0 78 2.5 79 1.5 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.08 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.475 #Duplication Level Percentage of deduplicated Percentage of total 1 91.51798771570635 78.225 2 5.001462415911085 8.55 3 1.6964024568587306 4.35 4 0.9066978648727698 3.1 5 0.26323486399532026 1.125 6 0.17548990933021352 0.8999999999999999 7 0.14624159110851126 0.8750000000000001 8 0.058496636443404505 0.4 9 0.0 0.0 >10 0.23398654577361802 2.475 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTATATCTCGTATGC 19 0.475 TruSeq Adapter, Index 15 (97% over 37bp) GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 14 0.35000000000000003 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 12 0.3 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 11 0.27499999999999997 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 11 0.27499999999999997 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 11 0.27499999999999997 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 11 0.27499999999999997 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 10 0.25 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 8 0.2 No Hit CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 8 0.2 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 7 0.17500000000000002 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 7 0.17500000000000002 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 7 0.17500000000000002 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 7 0.17500000000000002 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 7 0.17500000000000002 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 6 0.15 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 6 0.15 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 6 0.15 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 6 0.15 No Hit CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC 6 0.15 No Hit GCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTA 6 0.15 No Hit CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC 5 0.125 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTATATCTCGTATGCC 5 0.125 TruSeq Adapter, Index 15 (97% over 36bp) GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 5 0.125 No Hit CCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCA 5 0.125 No Hit GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA 5 0.125 No Hit CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT 5 0.125 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 5 0.125 No Hit GCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCT 5 0.125 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.7250000000000001 0.0 0.0 0.0 0.0 90-91 0.9625 0.0 0.0 0.0 0.0 92-93 1.1625 0.0 0.0 0.0 0.0 94-95 1.3625 0.0 0.0 0.0 0.0 96-97 1.6125 0.0 0.0 0.0 0.0 98-99 1.9125 0.0 0.0 0.0 0.0 100-101 2.0625 0.0 0.0 0.0 0.0 102-103 2.25 0.0 0.0 0.0 0.0 104-105 2.6375 0.0 0.0 0.0 0.0 106-107 3.2 0.0 0.0 0.0 0.0 108-109 3.5625 0.0 0.0 0.0 0.0 110-111 4.0 0.0 0.0 0.0 0.0 112-113 4.449999999999999 0.0 0.0 0.0 0.0 114-115 4.9875 0.0 0.0 0.0 0.0 116-117 5.5 0.0 0.0 0.0 0.0 118-119 6.074999999999999 0.0 0.0 0.0 0.0 120-121 6.55 0.0 0.0 0.0 0.0 122-123 7.0375 0.0 0.0 0.0 0.0 124-125 7.5 0.0 0.0 0.0 0.0 126-127 8.05 0.0 0.0 0.0 0.0 128-129 8.5625 0.0 0.0 0.0 0.0 130-131 9.087499999999999 0.0 0.0 0.0 0.0 132-133 9.462499999999999 0.0 0.0 0.0 0.0 134-135 10.0 0.0 0.0 0.0 0.0 136-137 10.600000000000001 0.0 0.0 0.0 0.0 138-139 11.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCAGAT 10 0.0065874006 146.74684 145 >>END_MODULE SRR7473327 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473327_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.1395 34.0 33.0 34.0 33.0 34.0 2 33.17325 34.0 33.0 34.0 33.0 34.0 3 33.112 34.0 33.0 34.0 33.0 34.0 4 33.119 34.0 33.0 34.0 33.0 34.0 5 33.129 34.0 33.0 34.0 33.0 34.0 6 37.1025 38.0 38.0 38.0 37.0 38.0 7 37.07725 38.0 38.0 38.0 37.0 38.0 8 37.05975 38.0 38.0 38.0 37.0 38.0 9 37.2045 38.0 38.0 38.0 37.0 38.0 10-14 37.13185 38.0 38.0 38.0 37.0 38.0 15-19 37.177949999999996 38.0 38.0 38.0 37.8 38.0 20-24 37.17895 38.0 38.0 38.0 38.0 38.0 25-29 37.2436 38.0 38.0 38.0 38.0 38.0 30-34 37.302350000000004 38.0 38.0 38.0 38.0 38.0 35-39 37.07725000000001 38.0 38.0 38.0 37.2 38.0 40-44 37.124399999999994 38.0 38.0 38.0 37.0 38.0 45-49 37.139849999999996 38.0 38.0 38.0 37.2 38.0 50-54 37.18185 38.0 38.0 38.0 37.4 38.0 55-59 37.161500000000004 38.0 38.0 38.0 37.4 38.0 60-64 37.1416 38.0 38.0 38.0 37.2 38.0 65-69 36.9712 38.0 38.0 38.0 37.0 38.0 70-74 36.81345 38.0 38.0 38.0 36.8 38.0 75-79 36.79705 38.0 38.0 38.0 36.6 38.0 80-84 36.70865 38.0 38.0 38.0 36.2 38.0 85-89 36.6865 38.0 38.0 38.0 36.0 38.0 90-94 36.55265 38.0 38.0 38.0 35.4 38.0 95-99 36.5233 38.0 38.0 38.0 35.4 38.0 100-104 36.3879 38.0 38.0 38.0 35.0 38.0 105-109 36.26035 38.0 38.0 38.0 34.4 38.0 110-114 35.985549999999996 38.0 38.0 38.0 34.0 38.0 115-119 35.56285 38.0 37.4 38.0 31.6 38.0 120-124 35.814049999999995 38.0 38.0 38.0 33.2 38.0 125-129 35.63595 38.0 37.8 38.0 32.8 38.0 130-134 35.22605 38.0 36.2 38.0 31.2 38.0 135-139 35.00285000000001 38.0 36.2 38.0 30.0 38.0 140-144 34.43305 38.0 35.6 38.0 27.2 38.0 145-149 33.73295 38.0 34.2 38.0 22.0 38.0 150-151 29.463625 35.5 27.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 3.0 4 1.0 5 1.0 6 0.0 7 2.0 8 1.0 9 0.0 10 0.0 11 5.0 12 5.0 13 7.0 14 1.0 15 2.0 16 4.0 17 16.0 18 9.0 19 4.0 20 10.0 21 6.0 22 12.0 23 12.0 24 10.0 25 16.0 26 14.0 27 16.0 28 23.0 29 26.0 30 36.0 31 38.0 32 55.0 33 62.0 34 102.0 35 160.0 36 461.0 37 2874.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.625 19.05 13.825000000000001 30.5 2 29.914957478739368 23.386693346673336 26.813406703351678 19.884942471235618 3 23.317488116087066 24.943707780835627 27.32049036777583 24.418313735301474 4 27.7027027027027 31.431431431431434 20.72072072072072 20.145145145145147 5 27.62071553665249 33.90042531898924 18.764073054791094 19.714786089567177 6 26.0 34.125 19.650000000000002 20.225 7 21.3 20.175 34.825 23.7 8 24.125 25.35 23.525 27.0 9 25.525 23.849999999999998 25.974999999999998 24.65 10-14 26.849027354103118 26.35895384307646 22.508376256438467 24.283642546381955 15-19 27.129069360404063 25.81387208081212 23.978596789518427 23.07846176926539 20-24 27.986399319965997 25.94129706485324 23.14615730786539 22.926146307315364 25-29 26.510302060412084 26.400280056011205 24.26985397079416 22.819563912782556 30-34 27.295 26.55 23.880000000000003 22.275 35-39 26.632663266326634 24.877487748774875 24.752475247524753 23.737373737373737 40-44 27.92639631981599 26.131306565328266 23.92119605980299 22.021101055052753 45-49 27.56413462019303 25.47882182327349 24.773716057408613 22.183327499124868 50-54 25.717571757175715 25.64756475647565 26.05260526052605 22.58225822582258 55-59 26.182618261826185 26.607660766076606 25.577557755775576 21.632163216321633 60-64 25.637691307392217 26.43292987896369 25.472641792537758 22.45673702110633 65-69 26.023011505752873 26.578289144572288 25.152576288144076 22.246123061530763 70-74 26.327632763276327 26.167616761676165 25.66756675667567 21.837183718371836 75-79 25.861293064653236 26.466323316165806 24.766238311915593 22.906145307265362 80-84 26.262626262626267 26.487648764876486 24.827482748274825 22.422242224222423 85-89 26.040000000000003 26.740000000000002 25.25 21.97 90-94 26.4252850570114 26.210242048409683 25.495099019803963 21.869373874774954 95-99 25.695 27.73 24.955 21.62 100-104 26.865 27.235 23.990000000000002 21.91 105-109 25.85887883182477 27.959193879081862 24.048607291093663 22.1333199979997 110-114 26.714042638374536 27.724952457211486 23.926533880492443 21.634471023921527 115-119 26.814859317112244 28.42695504155402 24.121357765094622 20.63682787623911 120-124 25.954083929375283 27.664682638923622 24.57860251087881 21.80263092082229 125-129 26.125 27.944999999999997 24.165 21.765 130-134 26.275 27.644999999999996 24.654999999999998 21.425 135-139 26.915 27.334999999999997 24.375 21.375 140-144 26.735 27.200000000000003 24.64 21.425 145-149 27.32 27.435 24.365000000000002 20.880000000000003 150-151 28.299999999999997 27.5125 24.2 19.9875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.5 20 1.0 21 1.5 22 2.0 23 2.0 24 2.0 25 1.0 26 2.5 27 5.0 28 10.0 29 12.0 30 13.5 31 23.5 32 25.5 33 26.0 34 48.0 35 68.5 36 78.5 37 89.0 38 93.5 39 103.0 40 120.5 41 116.0 42 105.0 43 103.0 44 111.5 45 120.5 46 114.5 47 121.0 48 141.0 49 170.0 50 170.0 51 154.0 52 183.5 53 221.0 54 229.5 55 212.5 56 175.0 57 137.5 58 118.0 59 97.5 60 70.0 61 58.5 62 56.5 63 48.5 64 32.0 65 24.5 66 26.5 67 29.5 68 27.0 69 18.5 70 15.0 71 13.5 72 13.0 73 12.0 74 8.5 75 5.0 76 3.0 77 3.0 78 1.5 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.075 4 0.1 5 0.075 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.015 20-24 0.005 25-29 0.02 30-34 0.0 35-39 0.01 40-44 0.005 45-49 0.015 50-54 0.01 55-59 0.01 60-64 0.03 65-69 0.05 70-74 0.01 75-79 0.005 80-84 0.01 85-89 0.0 90-94 0.02 95-99 0.0 100-104 0.0 105-109 0.015 110-114 0.09 115-119 0.13 120-124 0.034999999999999996 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.625 #Duplication Level Percentage of deduplicated Percentage of total 1 90.55306427503737 75.725 2 5.680119581464873 9.5 3 1.763826606875934 4.425 4 0.6278026905829596 2.1 5 0.35874439461883406 1.5 6 0.38863976083707025 1.95 7 0.32884902840059793 1.925 8 0.08968609865470852 0.6 9 0.05979073243647235 0.44999999999999996 >10 0.14947683109118087 1.825 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 20 0.5 Illumina Single End PCR Primer 1 (100% over 50bp) CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 19 0.475 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 12 0.3 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 11 0.27499999999999997 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 11 0.27499999999999997 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 9 0.22499999999999998 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 9 0.22499999999999998 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 8 0.2 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 8 0.2 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 8 0.2 No Hit AATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCA 7 0.17500000000000002 No Hit TGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCC 7 0.17500000000000002 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 7 0.17500000000000002 No Hit CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT 7 0.17500000000000002 No Hit CCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT 7 0.17500000000000002 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 7 0.17500000000000002 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 7 0.17500000000000002 No Hit GTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGG 7 0.17500000000000002 No Hit GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG 7 0.17500000000000002 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 7 0.17500000000000002 No Hit GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC 7 0.17500000000000002 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 6 0.15 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 6 0.15 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 6 0.15 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 6 0.15 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 6 0.15 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 6 0.15 No Hit AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA 6 0.15 No Hit GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGAT 6 0.15 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 6 0.15 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 6 0.15 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 6 0.15 No Hit CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC 6 0.15 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 6 0.15 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 5 0.125 No Hit GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT 5 0.125 No Hit CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG 5 0.125 No Hit CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG 5 0.125 No Hit AAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA 5 0.125 No Hit AGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGC 5 0.125 No Hit CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT 5 0.125 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 5 0.125 No Hit CTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATTAACCA 5 0.125 No Hit GGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATG 5 0.125 No Hit GGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGA 5 0.125 No Hit GGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.5375 0.0 0.0 0.0 0.0 88-89 0.7 0.0 0.0 0.0 0.0 90-91 0.9375 0.0 0.0 0.0 0.0 92-93 1.1375 0.0 0.0 0.0 0.0 94-95 1.325 0.0 0.0 0.0 0.0 96-97 1.5499999999999998 0.0 0.0 0.0 0.0 98-99 1.825 0.0 0.0 0.0 0.0 100-101 1.9625 0.0 0.0 0.0 0.0 102-103 2.1375 0.0 0.0 0.0 0.0 104-105 2.5125 0.0 0.0 0.0 0.0 106-107 3.05 0.0 0.0 0.0 0.0 108-109 3.375 0.0 0.0 0.0 0.0 110-111 3.825 0.0 0.0 0.0 0.0 112-113 4.2625 0.0 0.0 0.0 0.0 114-115 4.7875 0.0 0.0 0.0 0.0 116-117 5.325 0.0 0.0 0.0 0.0 118-119 5.9 0.0 0.0 0.0 0.0 120-121 6.375 0.0 0.0 0.0 0.0 122-123 6.8625 0.0 0.0 0.0 0.0 124-125 7.324999999999999 0.0 0.0 0.0 0.0 126-127 7.875 0.0 0.0 0.0 0.0 128-129 8.4 0.0 0.0 0.0 0.0 130-131 8.9375 0.0 0.0 0.0 0.0 132-133 9.3875 0.0 0.0 0.0 0.0 134-135 9.9 0.0 0.0 0.0 0.0 136-137 10.5 0.0 0.0 0.0 0.0 138-139 11.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTTCT 10 0.006830828 145.0 4 >>END_MODULE Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 873003 spots for SRR7473327.sra Written 873003 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra Read 872986 spots for SRR7473327.sra Written 872986 spots for SRR7473327.sra SRR ids: ['SRR7473327.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ixbjli6i SRR7473327.sra spots: 17459737 blocks: [[1, 872986], [872987, 1745972], [1745973, 2618958], [2618959, 3491944], [3491945, 4364930], [4364931, 5237916], [5237917, 6110902], [6110903, 6983888], [6983889, 7856874], [7856875, 8729860], [8729861, 9602846], [9602847, 10475832], [10475833, 11348818], [11348819, 12221804], [12221805, 13094790], [13094791, 13967776], [13967777, 14840762], [14840763, 15713748], [15713749, 16586734], [16586735, 17459737]] SRR7473327 file size 5894831 SRR7473327 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473327 SRR7473327_1.fastq SRR7473327_2.fastq Input file: SRR7473327_1.fastq Paired file: SRR7473327_2.fastq trimmed: SRR7473327-trimmed-pair1.fastq, SRR7473327-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 13:32:39 2024 >> started Sat Dec 7 13:33:00 2024 >> done (21.281s) 17459737 read pairs processed; of these: 40924 ( 0.23%) short read pairs filtered out after trimming by size control 112419 ( 0.64%) empty read pairs filtered out after trimming by size control 17306394 (99.12%) read pairs available; of these: 8120521 (46.92%) trimmed read pairs available after processing 9185873 (53.08%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 10 0.00% 20 13 0.00% 21 18 0.00% 22 23 0.00% 23 17 0.00% 24 17 0.00% 25 15 0.00% 26 22 0.00% 27 30 0.00% 28 29 0.00% 29 34 0.00% 30 47 0.00% 31 46 0.00% 32 39 0.00% 33 47 0.00% 34 55 0.00% 35 56 0.00% 36 51 0.00% 37 59 0.00% 38 63 0.00% 39 94 0.00% 40 89 0.00% 41 134 0.00% 42 126 0.00% 43 112 0.00% 44 125 0.00% 45 163 0.00% 46 187 0.00% 47 197 0.00% 48 230 0.00% 49 246 0.00% 50 249 0.00% 51 282 0.00% 52 346 0.00% 53 315 0.00% 54 375 0.00% 55 364 0.00% 56 406 0.00% 57 465 0.00% 58 498 0.00% 59 548 0.00% 60 657 0.00% 61 704 0.00% 62 780 0.00% 63 953 0.01% 64 1069 0.01% 65 1328 0.01% 66 1589 0.01% 67 1995 0.01% 68 4365 0.03% 69 18225 0.11% 70 27268 0.16% 71 11036 0.06% 72 5610 0.03% 73 4727 0.03% 74 4359 0.03% 75 4139 0.02% 76 3982 0.02% 77 4342 0.03% 78 4497 0.03% 79 5303 0.03% 80 5561 0.03% 81 6055 0.03% 82 7184 0.04% 83 8339 0.05% 84 11705 0.07% 85 12946 0.07% 86 14281 0.08% 87 15348 0.09% 88 17441 0.10% 89 17158 0.10% 90 18633 0.11% 91 18807 0.11% 92 18588 0.11% 93 22556 0.13% 94 22807 0.13% 95 26541 0.15% 96 25748 0.15% 97 26725 0.15% 98 25913 0.15% 99 26817 0.15% 100 30169 0.17% 101 28454 0.16% 102 30347 0.18% 103 30630 0.18% 104 33418 0.19% 105 38087 0.22% 106 36834 0.21% 107 36063 0.21% 108 38765 0.22% 109 45152 0.26% 110 46880 0.27% 111 41743 0.24% 112 42526 0.25% 113 52515 0.30% 114 47614 0.28% 115 51734 0.30% 116 53967 0.31% 117 50722 0.29% 118 51389 0.30% 119 51909 0.30% 120 54479 0.31% 121 51463 0.30% 122 56271 0.33% 123 58777 0.34% 124 59229 0.34% 125 59598 0.34% 126 60312 0.35% 127 62045 0.36% 128 61834 0.36% 129 63476 0.37% 130 66890 0.39% 131 65729 0.38% 132 68440 0.40% 133 71949 0.42% 134 75422 0.44% 135 78131 0.45% 136 79118 0.46% 137 87098 0.50% 138 88335 0.51% 139 91900 0.53% 140 91459 0.53% 141 102013 0.59% 142 105481 0.61% 143 111258 0.64% 144 123176 0.71% 145 139376 0.81% 146 164940 0.95% 147 208710 1.21% 148 300980 1.74% 149 591300 3.42% 150 3520083 20.34% 151 9185873 53.08% 17306394 reads passed initial QC criterion=sequence-density sequence-density=0.55 sequence-density-rank=1 fanout-score=6.60 fanout-score-rank=7 prefix-density=2.06 prefix-fanout=1.8 sequence=CACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=10.59 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=2.0 sequence=GCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACCCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTAT criterion=sequence-density sequence-density=2.57 sequence-density-rank=1 fanout-score=2.33 fanout-score-rank=29 prefix-density=2.75 prefix-fanout=2.2 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=71.08 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=1.1 sequence=TTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTTGGTGTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCCTATTGTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGAATTTGTGGAGTGAGAGGATTAAAGATAGGGAAGTTGTTGAAGTTATTGGCTGTGAGAGAGTGCCATTGATGAA SRR7473327 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 13:34:37 Started mapping on | Dec 07 13:34:37 Finished on | Dec 07 13:59:59 Mapping speed, Million of reads per hour | 40.93 Number of input reads | 17306394 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 8337681 Uniquely mapped reads % | 48.18% Average mapped length | 293.06 Number of splices: Total | 8796638 Number of splices: Annotated (sjdb) | 8224761 Number of splices: GT/AG | 8680945 Number of splices: GC/AG | 101993 Number of splices: AT/AC | 5232 Number of splices: Non-canonical | 8468 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.01% Deletion average length | 1.45 Insertion rate per base | 0.01% Insertion average length | 1.50 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 124347 % of reads mapped to multiple loci | 0.72% Number of reads mapped to too many loci | 10841 % of reads mapped to too many loci | 0.06% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 49.27% % of reads unmapped: other | 1.77% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8858356 8858356 8858356 N_multimapping 124347 124347 124347 N_noFeature 336540 8038527 438433 N_ambiguous 235090 1455 38029 UnstrandedReadsAssigned:7766051 PositiveStrandReadsAssigned:297699 NegativeStrandReadsAssigned:7861219 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR7473327 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473327-trimmed-pair1.fastq SRR7473327-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,306,394 reads, 8,036,029 reads pseudoaligned [quant] estimated average fragment length: 253.939 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,085 rounds 52973 SRR7473327.ke.tsv 35125 SRR7473327.se.tsv 88098 total ==> SRR7473327.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 683.731 0 0 PNS24247 1044 791.061 13.251 2.51742 PNS24249 1928 1675.06 28.0399 2.51572 PNS24246 1044 791.061 13.251 2.51742 PNS24248 1044 791.061 13.251 2.51742 PNS24244 1471 1218.06 30.2072 3.727 PNS24243 293 98.4583 0 0 KQK14069 1603 1350.06 392.356 43.6762 KQK14071 474 242.097 14.849 9.21781 ==> SRR7473327.se.tsv <== BRADI_1g14170v3 448 BRADI_1g53295v3 23 BRADI_1g59795v3 311 BRADI_1g07683v3 0 BRADI_1g00485v3 11 BRADI_1g20270v3 1284 BRADI_1g74790v3 82 BRADI_1g09890v3 2 BRADI_1g77505v3 233 BRADI_1g48960v3 0 SRR7473327 completed mapping pipeline successfully