Starting /dee2/code/volunteer_pipeline.sh SRR7473328 current disk space = 1543104851968 free memory = 1596607076 SRR7473328 SRAfilesize 293a6ad832a152a4eb90d65c7d255d32 SRR7473328.sra SRR7473328.sra file validated SRR7473328 is paired end SRR7473328 is conventional basespace SRR7473328 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473328_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.4425 34.0 34.0 34.0 33.0 34.0 2 33.48025 34.0 34.0 34.0 33.0 34.0 3 33.5045 34.0 34.0 34.0 33.0 34.0 4 33.52775 34.0 34.0 34.0 33.0 34.0 5 33.568 34.0 34.0 34.0 33.0 34.0 6 37.12525 38.0 37.0 38.0 36.0 38.0 7 37.40725 38.0 38.0 38.0 37.0 38.0 8 37.50825 38.0 38.0 38.0 38.0 38.0 9 37.60425 38.0 38.0 38.0 38.0 38.0 10-14 37.5938 38.0 38.0 38.0 38.0 38.0 15-19 37.634550000000004 38.0 38.0 38.0 37.8 38.0 20-24 37.58645 38.0 38.0 38.0 37.8 38.0 25-29 37.5228 38.0 38.0 38.0 38.0 38.0 30-34 37.4333 38.0 38.0 38.0 37.8 38.0 35-39 37.34920000000001 38.0 38.0 38.0 37.6 38.0 40-44 36.452 38.0 37.6 38.0 33.0 38.0 45-49 37.1854 38.0 38.0 38.0 36.8 38.0 50-54 37.202099999999994 38.0 38.0 38.0 37.0 38.0 55-59 37.2611 38.0 38.0 38.0 37.0 38.0 60-64 37.12455 38.0 38.0 38.0 36.4 38.0 65-69 36.9394 38.0 38.0 38.0 35.8 38.0 70-74 35.86280000000001 38.0 37.6 38.0 28.8 38.0 75-79 31.09345 38.0 35.8 38.0 2.0 38.0 80-84 30.9663 38.0 35.2 38.0 2.0 38.0 85-89 30.852300000000003 38.0 35.0 38.0 2.0 38.0 90-94 30.6005 38.0 34.2 38.0 2.0 38.0 95-99 30.36365 38.0 33.8 38.0 2.0 38.0 100-104 30.241799999999994 38.0 33.2 38.0 2.0 38.0 105-109 30.080450000000003 38.0 32.6 38.0 2.0 38.0 110-114 29.913599999999995 38.0 31.8 38.0 2.0 38.0 115-119 29.730499999999996 38.0 30.2 38.0 2.0 38.0 120-124 29.4921 38.0 29.2 38.0 2.0 38.0 125-129 29.198500000000003 38.0 27.2 38.0 2.0 38.0 130-134 28.692700000000002 38.0 23.8 38.0 2.0 38.0 135-139 28.315800000000003 38.0 22.2 38.0 2.0 38.0 140-144 27.8833 37.2 16.4 38.0 2.0 38.0 145-149 26.838750000000005 36.0 8.4 38.0 2.0 38.0 150-151 22.55775 30.5 2.0 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 0.0 11 0.0 12 2.0 13 3.0 14 5.0 15 11.0 16 13.0 17 40.0 18 166.0 19 450.0 20 23.0 21 23.0 22 8.0 23 11.0 24 15.0 25 21.0 26 14.0 27 23.0 28 21.0 29 34.0 30 31.0 31 45.0 32 53.0 33 81.0 34 110.0 35 191.0 36 584.0 37 2020.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.707653701380174 9.611041405269761 8.682559598494354 32.99874529485571 2 20.075000000000003 29.049999999999997 26.35 24.525 3 17.974999999999998 14.649999999999999 36.175000000000004 31.2 4 22.05 19.975 16.425 41.55 5 40.25 22.55 18.4 18.8 6 35.099999999999994 24.975 20.175 19.75 7 14.124999999999998 35.425000000000004 33.125 17.325 8 16.2 35.625 23.625 24.55 9 32.125 17.349999999999998 27.925 22.6 10-14 21.865000000000002 27.98 20.24 29.915000000000003 15-19 21.92 23.755000000000003 25.174999999999997 29.15 20-24 22.175 27.584999999999997 24.145 26.095000000000002 25-29 21.645 24.165 24.6 29.59 30-34 22.235 23.294999999999998 24.64 29.830000000000002 35-39 22.355 27.415 24.615000000000002 25.615 40-44 18.795675242767043 23.19551506657323 28.416257883672042 29.59255180698769 45-49 25.52127606380319 23.186159307965397 28.41142057102855 22.88114405720286 50-54 21.89 19.994999999999997 24.595 33.52 55-59 21.945 20.16 31.7 26.195 60-64 21.775 23.11 28.634999999999998 26.479999999999997 65-69 18.41 37.724999999999994 20.76 23.105 70-74 18.915000000000003 38.135000000000005 20.635 22.314999999999998 75-79 19.535 35.885 20.995 23.585 80-84 20.990000000000002 31.31 23.455000000000002 24.245 85-89 22.040000000000003 27.47 23.31 27.18 90-94 21.931579473842152 25.66269880964289 25.59767930379114 26.808042412723815 95-99 21.59847764034253 26.325804997746506 25.870098652912016 26.205618708998944 100-104 20.685000000000002 32.7 22.785 23.830000000000002 105-109 19.8 34.5 21.11 24.59 110-114 20.69 33.455 21.310000000000002 24.545 115-119 20.715 31.724999999999998 21.595 25.965 120-124 21.065 30.880000000000003 21.94 26.115 125-129 22.229462182346747 29.517317427697858 22.80587439226104 25.447345997694352 130-134 21.474955952680595 29.755852001006794 22.92977598791845 25.83941605839416 135-139 21.91511856215073 29.889744751548104 22.332980919297185 25.862155767003976 140-144 22.86531800612419 29.576828472466243 21.956729079865468 25.601124441544098 145-149 22.959645768340543 28.9121465230955 22.038844721747004 26.089362986816944 150-151 21.664149043303123 29.99748237663645 22.192849949647535 26.14551863041289 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.5 22 1.0 23 1.0 24 0.5 25 0.5 26 1.5 27 1.0 28 1.0 29 1.5 30 4.5 31 9.0 32 12.0 33 17.0 34 24.5 35 42.5 36 61.5 37 86.0 38 107.0 39 137.5 40 175.5 41 180.5 42 175.5 43 175.5 44 171.5 45 165.5 46 148.5 47 131.5 48 135.5 49 140.0 50 141.5 51 142.5 52 147.0 53 167.5 54 187.5 55 184.0 56 151.0 57 130.5 58 113.5 59 83.0 60 76.5 61 63.0 62 43.5 63 36.5 64 30.0 65 26.5 66 25.0 67 24.0 68 19.0 69 15.0 70 18.0 71 18.0 72 11.5 73 6.0 74 5.5 75 5.5 76 4.0 77 4.5 78 3.0 79 1.0 80 1.0 81 1.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.11 45-49 0.005 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.03 95-99 0.155 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.245 130-134 0.675 135-139 0.685 140-144 0.395 145-149 0.63 150-151 0.7000000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.25 #Duplication Level Percentage of deduplicated Percentage of total 1 93.06397306397307 69.1 2 3.939393939393939 5.8500000000000005 3 1.4478114478114479 3.225 4 0.7070707070707071 2.1 5 0.3703703703703704 1.375 6 0.16835016835016833 0.75 7 0.10101010101010101 0.525 8 0.03367003367003367 0.2 9 0.06734006734006734 0.44999999999999996 >10 0.06734006734006734 0.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.03367003367003367 15.9 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC 636 15.9 TruSeq Adapter, Index 9 (100% over 50bp) CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 11 0.27499999999999997 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 10 0.25 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 9 0.22499999999999998 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 9 0.22499999999999998 No Hit GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 8 0.2 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 7 0.17500000000000002 No Hit CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC 7 0.17500000000000002 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC 7 0.17500000000000002 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 6 0.15 No Hit GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC 6 0.15 TruSeq Adapter, Index 9 (98% over 50bp) GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA 6 0.15 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 6 0.15 No Hit CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT 5 0.125 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 5 0.125 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 5 0.125 No Hit GATCGGAAGACACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC 5 0.125 TruSeq Adapter, Index 9 (100% over 40bp) CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 5 0.125 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 5 0.125 No Hit CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCA 5 0.125 No Hit GGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGAT 5 0.125 No Hit ACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTC 5 0.125 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 5 0.125 No Hit CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.375 0.0 0.0 0.0 0.0 90-91 0.45 0.0 0.0 0.0 0.0 92-93 0.6625 0.0 0.0 0.0 0.0 94-95 0.8125 0.0 0.0 0.0 0.0 96-97 1.025 0.0 0.0 0.0 0.0 98-99 1.2625000000000002 0.0 0.0 0.0 0.0 100-101 1.35 0.0 0.0 0.0 0.0 102-103 1.525 0.0 0.0 0.0 0.0 104-105 1.8 0.0 0.0 0.0 0.0 106-107 2.1375 0.0 0.0 0.0 0.0 108-109 2.375 0.0 0.0 0.0 0.0 110-111 2.7125 0.0 0.0 0.0 0.0 112-113 3.0125 0.0 0.0 0.0 0.0 114-115 3.5875000000000004 0.0 0.0 0.0 0.0 116-117 4.075 0.0 0.0 0.0 0.0 118-119 4.525 0.0 0.0 0.0 0.0 120-121 4.8875 0.0 0.0 0.0 0.0 122-123 5.25 0.0 0.0 0.0 0.0 124-125 5.7375 0.0 0.0 0.0 0.0 126-127 6.2875 0.0 0.0 0.0 0.0 128-129 6.9 0.0 0.0 0.0 0.0 130-131 7.4125 0.0 0.0 0.0 0.0 132-133 7.949999999999999 0.0 0.0 0.0 0.0 134-135 8.5625 0.0 0.0 0.0 0.0 136-137 9.1875 0.0 0.0 0.0 0.0 138-139 9.837499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCAC 105 0.0 82.58572 8 AAGAGCA 110 0.0 78.83182 7 GAAGAGC 110 0.0 78.83182 6 TCGGAAG 115 0.0 75.40435 3 GAGCACA 115 0.0 75.40435 9 CGGAAGA 115 0.0 75.40435 4 ATCGGAA 115 0.0 75.40435 2 GGAAGAG 115 0.0 75.40435 5 GATCGGA 125 0.0 70.25013 1 CACGAGC 20 0.006031041 28.905 30-34 TACATAT 20 0.006031041 28.905 50-54 GACTGAT 20 0.006031041 28.905 15-19 CGTGCCT 20 0.006031041 28.905 60-64 TCACCTA 20 0.006031041 28.905 45-49 TATGCCG 65 4.3655746E-11 26.68154 45-49 GAAAAAA 65 4.3655746E-11 26.68154 60-64 ATGCCGT 65 4.3655746E-11 26.68154 45-49 GTCTTCT 65 4.3655746E-11 26.68154 50-54 CGTATGC 65 4.3655746E-11 26.68154 40-44 GCTTGAA 65 4.3655746E-11 26.68154 55-59 >>END_MODULE SRR7473328 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473328_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2615 33.0 33.0 34.0 32.0 34.0 2 32.304 34.0 33.0 34.0 32.0 34.0 3 32.40475 34.0 33.0 34.0 32.0 34.0 4 32.35775 34.0 33.0 34.0 32.0 34.0 5 32.488 34.0 33.0 34.0 32.0 34.0 6 36.219 38.0 38.0 38.0 36.0 38.0 7 36.2 38.0 38.0 38.0 35.0 38.0 8 36.3955 38.0 38.0 38.0 35.0 38.0 9 36.5205 38.0 38.0 38.0 36.0 38.0 10-14 36.498450000000005 38.0 38.0 38.0 36.2 38.0 15-19 36.27845 38.0 38.0 38.0 35.8 38.0 20-24 36.054500000000004 38.0 38.0 38.0 35.6 38.0 25-29 36.0812 38.0 38.0 38.0 35.6 38.0 30-34 35.98065 38.0 38.0 38.0 34.6 38.0 35-39 35.8597 38.0 38.0 38.0 34.2 38.0 40-44 35.891099999999994 38.0 38.0 38.0 34.4 38.0 45-49 35.343 38.0 38.0 38.0 30.6 38.0 50-54 35.4434 38.0 37.8 38.0 31.2 38.0 55-59 35.755799999999994 38.0 38.0 38.0 33.8 38.0 60-64 35.817750000000004 38.0 38.0 38.0 34.0 38.0 65-69 33.65405 38.0 36.6 38.0 21.0 38.0 70-74 30.351549999999996 38.0 34.2 38.0 2.0 38.0 75-79 30.307049999999997 38.0 34.4 38.0 2.0 38.0 80-84 30.217599999999997 38.0 34.0 38.0 2.0 38.0 85-89 30.15625 38.0 34.0 38.0 2.0 38.0 90-94 29.956449999999997 38.0 33.2 38.0 2.0 38.0 95-99 29.672250000000002 38.0 32.2 38.0 2.0 38.0 100-104 29.13725 38.0 26.6 38.0 2.0 38.0 105-109 29.0482 38.0 26.2 38.0 2.0 38.0 110-114 28.812450000000002 38.0 23.0 38.0 2.0 38.0 115-119 28.6286 38.0 20.6 38.0 2.0 38.0 120-124 28.568899999999996 38.0 21.0 38.0 2.0 38.0 125-129 28.293349999999997 38.0 18.6 38.0 2.0 38.0 130-134 27.929949999999998 38.0 13.0 38.0 2.0 38.0 135-139 27.4724 38.0 12.2 38.0 2.0 38.0 140-144 26.788800000000002 37.6 3.8 38.0 2.0 38.0 145-149 25.5433 36.0 2.0 38.0 2.0 38.0 150-151 20.8405 26.5 2.0 35.5 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61.0 3 30.0 4 19.0 5 9.0 6 0.0 7 3.0 8 5.0 9 2.0 10 2.0 11 8.0 12 7.0 13 9.0 14 28.0 15 44.0 16 73.0 17 467.0 18 30.0 19 23.0 20 15.0 21 11.0 22 11.0 23 19.0 24 19.0 25 30.0 26 17.0 27 17.0 28 21.0 29 37.0 30 36.0 31 38.0 32 56.0 33 76.0 34 110.0 35 193.0 36 516.0 37 1958.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.82224479431183 13.941086846114779 11.858811579481971 24.377856780091417 2 26.891823260538345 35.44946673438293 21.254443880142205 16.404266124936516 3 22.100989596549102 20.223293580309566 37.249429078913984 20.426287744227352 4 24.829157175398635 25.841559098962286 15.439129334345736 33.89015439129334 5 41.92161820480405 26.49810366624526 15.06953223767383 16.510745891276866 6 38.296787353391124 29.39826619071902 15.272819989801123 17.03212646608873 7 19.959370238699847 32.960893854748605 27.882173692229557 19.19756221432199 8 22.741039878849065 35.436648157496215 18.702675416456334 23.119636547198386 9 39.500882278800105 19.813461053692965 19.737837156541467 20.947819510965466 10-14 30.31202818319074 24.559637644690486 21.12229491696024 24.00603925515853 15-19 30.02286004572009 21.341122682245363 26.04013208026416 22.595885191770385 20-24 33.85118560915781 27.718724448078497 19.061733442354864 19.368356500408833 25-29 29.347051033920753 31.053274931241724 19.44076601813181 20.158908016705716 30-34 30.15387750942627 24.844593906043006 25.66493427086518 19.336594313665543 35-39 24.018581857164737 24.743478482821992 25.208024911940374 26.029914748072898 40-44 36.85175751400917 20.825267447784004 22.699949057564954 19.623025980641877 45-49 27.114911921343708 21.51782056534207 22.823637853338795 28.543629659975423 50-54 26.384497705252425 24.660887302396738 25.991840897501273 22.962774094849568 55-59 23.37728452883979 31.400498905462506 26.23835463014814 18.98386193554956 60-64 23.169672508046798 37.65391099984673 19.8640985030399 19.31231798906657 65-69 23.715965346534652 37.85581683168317 19.425536303630363 19.002681518151814 70-74 24.829238454480578 35.548985625446015 20.25180956264655 19.36996635742685 75-79 25.49469604243166 33.6750305997552 20.506935944512446 20.323337413300692 80-84 26.56329694991329 31.93920228501479 21.350606957053962 20.146893808017953 85-89 26.992928727679704 29.76039070051381 22.348272879890114 20.898407691916365 90-94 27.691051933476174 29.971431486583 22.07937965513723 20.25813692480359 95-99 26.602151644618317 29.881093323724713 21.87676944458743 21.639985587069543 100-104 26.819343732067402 30.11111690750691 22.379884188011896 20.689655172413794 105-109 26.012193215569802 31.139596685946536 21.489239747798447 21.358970350685215 110-114 26.889178617992176 30.503259452411996 21.814863102998697 20.79269882659713 115-119 26.599501454092234 30.473618612380555 21.93601994183631 20.990859991690904 120-124 26.60088288756167 30.27784990911452 22.041028304336535 21.080238898987275 125-129 26.46676713610144 30.353894922829085 21.91446240191238 21.264875539157096 130-134 26.710334788937406 30.11021002287378 21.89124558120191 21.2882096069869 135-139 27.467411545623836 29.500310366232153 22.01531140078626 21.016966687357748 140-144 27.547540305911532 29.066763125258372 22.075237701529556 21.31045886730054 145-149 27.329968257272206 29.666441171879065 21.793203934016756 21.21038663683197 150-151 28.750981932443047 30.125687352710134 20.686043466876143 20.437287247970673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14.0 1 11.0 2 5.5 3 5.5 4 7.0 5 5.5 6 4.5 7 3.0 8 2.0 9 1.5 10 2.0 11 4.0 12 3.5 13 1.5 14 3.0 15 3.5 16 2.0 17 1.5 18 1.0 19 0.5 20 1.0 21 2.0 22 2.5 23 2.0 24 1.0 25 2.0 26 2.5 27 3.0 28 4.5 29 6.5 30 9.5 31 15.0 32 20.0 33 32.0 34 54.5 35 76.0 36 86.5 37 88.5 38 102.0 39 110.0 40 112.0 41 124.5 42 127.5 43 132.0 44 131.0 45 126.5 46 125.0 47 139.0 48 143.0 49 138.5 50 155.5 51 155.5 52 152.0 53 177.5 54 180.0 55 172.0 56 161.0 57 121.5 58 96.0 59 85.0 60 85.5 61 81.0 62 70.5 63 54.5 64 33.0 65 24.0 66 27.0 67 32.0 68 30.5 69 21.0 70 15.0 71 16.0 72 15.0 73 9.5 74 8.0 75 6.5 76 4.5 77 5.5 78 5.0 79 2.0 80 1.0 81 1.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.55 2 1.55 3 1.4749999999999999 4 1.225 5 1.125 6 1.95 7 1.55 8 0.95 9 0.8250000000000001 10-14 0.65 15-19 1.575 20-24 2.16 25-29 1.83 30-34 1.87 35-39 2.0549999999999997 40-44 1.8499999999999999 45-49 2.36 50-54 1.95 55-59 1.7850000000000001 60-64 2.1350000000000002 65-69 3.04 70-74 1.91 75-79 1.96 80-84 1.97 85-89 1.7149999999999999 90-94 1.9900000000000002 95-99 2.8649999999999998 100-104 4.154999999999999 105-109 4.045 110-114 4.125 115-119 3.7199999999999998 120-124 3.7249999999999996 125-129 3.785 130-134 3.82 135-139 3.34 140-144 3.2399999999999998 145-149 3.9149999999999996 150-151 4.5249999999999995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.64999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 93.82022471910112 70.975 2 3.866490416391276 5.8500000000000005 3 1.0244547257105088 2.325 4 0.39656311962987445 1.2 5 0.3304692663582287 1.25 6 0.19828155981493722 0.8999999999999999 7 0.033046926635822864 0.17500000000000002 8 0.19828155981493722 1.2 9 0.033046926635822864 0.22499999999999998 >10 0.06609385327164573 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.033046926635822864 15.4 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 616 15.4 Illumina Single End PCR Primer 1 (100% over 50bp) GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 10 0.25 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 10 0.25 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 9 0.22499999999999998 No Hit CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 8 0.2 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 8 0.2 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 8 0.2 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 8 0.2 No Hit CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG 8 0.2 No Hit CGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAG 8 0.2 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7 0.17500000000000002 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 6 0.15 No Hit GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 6 0.15 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 6 0.15 No Hit CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAG 6 0.15 No Hit TGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGG 6 0.15 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 6 0.15 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 5 0.125 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG 5 0.125 Illumina Single End PCR Primer 1 (98% over 50bp) GGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATG 5 0.125 No Hit GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG 5 0.125 No Hit GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG 5 0.125 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCCG 5 0.125 Illumina Single End PCR Primer 1 (98% over 50bp) CGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGA 5 0.125 No Hit NNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5 0.125 No Hit GGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC 5 0.125 No Hit CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.6000000000000001 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.95 0.0 0.0 0.0 0.0 98-99 1.1625 0.0 0.0 0.0 0.0 100-101 1.25 0.0 0.0 0.0 0.0 102-103 1.4 0.0 0.0 0.0 0.0 104-105 1.65 0.0 0.0 0.0 0.0 106-107 1.9375 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.4749999999999996 0.0 0.0 0.0 0.0 112-113 2.7249999999999996 0.0 0.0 0.0 0.0 114-115 3.3125 0.0 0.0 0.0 0.0 116-117 3.8 0.0 0.0 0.0 0.0 118-119 4.2125 0.0 0.0 0.0 0.0 120-121 4.55 0.0 0.0 0.0 0.0 122-123 4.9 0.0 0.0 0.0 0.0 124-125 5.3375 0.0 0.0 0.0 0.0 126-127 5.875 0.0 0.0 0.0 0.0 128-129 6.425 0.0 0.0 0.0 0.0 130-131 6.9375 0.0 0.0 0.0 0.0 132-133 7.4625 0.0 0.0 0.0 0.0 134-135 8.0125 0.0 0.0 0.0 0.0 136-137 8.4875 0.0 0.0 0.0 0.0 138-139 9.1125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCAGTT 10 0.006856144 144.79747 1 GAGCGTC 85 0.0 85.17498 9 AGAGCGT 90 0.0 80.44304 8 TCGGAAG 95 0.0 76.2092 3 CGGAAGA 100 0.0 72.398735 4 ATCGGAA 100 0.0 72.398735 2 AAGAGCG 105 0.0 68.95118 7 GAAGAGC 105 0.0 68.95118 6 GATCGGA 110 0.0 65.81703 1 GGAAGAG 120 0.0 60.332275 5 TTAAAAA 55 6.039045E-9 26.326813 55-59 CATTAAA 50 7.1466275E-8 26.063543 50-54 TCGCCGT 50 7.1466275E-8 26.063543 45-49 ATTAAAA 50 7.1466275E-8 26.063543 55-59 CGTATCA 45 8.477582E-7 25.741772 45-49 TGGTCGC 60 1.5250407E-8 24.13291 40-44 GTCGCCG 60 1.5250407E-8 24.13291 40-44 TAAAAAA 60 1.5250407E-8 24.13291 55-59 GGTCGCC 60 1.5250407E-8 24.13291 40-44 CCGTATC 55 1.7979255E-7 23.694132 45-49 >>END_MODULE Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029688 spots for SRR7473328.sra Written 1029688 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra Read 1029681 spots for SRR7473328.sra Written 1029681 spots for SRR7473328.sra SRR ids: ['SRR7473328.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8w4v_219 SRR7473328.sra spots: 20593627 blocks: [[1, 1029681], [1029682, 2059362], [2059363, 3089043], [3089044, 4118724], [4118725, 5148405], [5148406, 6178086], [6178087, 7207767], [7207768, 8237448], [8237449, 9267129], [9267130, 10296810], [10296811, 11326491], [11326492, 12356172], [12356173, 13385853], [13385854, 14415534], [14415535, 15445215], [15445216, 16474896], [16474897, 17504577], [17504578, 18534258], [18534259, 19563939], [19563940, 20593627]] SRR7473328 file size 6956804 SRR7473328 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473328 SRR7473328_1.fastq SRR7473328_2.fastq Input file: SRR7473328_1.fastq Paired file: SRR7473328_2.fastq trimmed: SRR7473328-trimmed-pair1.fastq, SRR7473328-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 13:32:42 2024 >> started Sat Dec 7 13:33:06 2024 >> done (23.889s) 20593627 read pairs processed; of these: 56894 ( 0.28%) short read pairs filtered out after trimming by size control 3731660 (18.12%) empty read pairs filtered out after trimming by size control 16805073 (81.60%) read pairs available; of these: 10079671 (59.98%) trimmed read pairs available after processing 6725402 (40.02%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 14 0.00% 20 10 0.00% 21 15 0.00% 22 20 0.00% 23 12 0.00% 24 25 0.00% 25 14 0.00% 26 16 0.00% 27 15 0.00% 28 23 0.00% 29 30 0.00% 30 26 0.00% 31 42 0.00% 32 31 0.00% 33 31 0.00% 34 48 0.00% 35 52 0.00% 36 64 0.00% 37 72 0.00% 38 72 0.00% 39 63 0.00% 40 83 0.00% 41 91 0.00% 42 139 0.00% 43 207 0.00% 44 370 0.00% 45 747 0.00% 46 1043 0.01% 47 1016 0.01% 48 895 0.01% 49 924 0.01% 50 853 0.01% 51 877 0.01% 52 1026 0.01% 53 1045 0.01% 54 996 0.01% 55 1203 0.01% 56 1125 0.01% 57 1171 0.01% 58 1169 0.01% 59 1159 0.01% 60 1252 0.01% 61 1347 0.01% 62 1230 0.01% 63 1374 0.01% 64 1389 0.01% 65 1672 0.01% 66 1941 0.01% 67 2579 0.02% 68 3741 0.02% 69 9432 0.06% 70 14909 0.09% 71 9765 0.06% 72 7159 0.04% 73 5900 0.04% 74 5008 0.03% 75 4636 0.03% 76 4439 0.03% 77 4893 0.03% 78 5261 0.03% 79 6018 0.04% 80 6532 0.04% 81 7012 0.04% 82 8013 0.05% 83 9374 0.06% 84 11843 0.07% 85 12339 0.07% 86 13972 0.08% 87 14915 0.09% 88 17440 0.10% 89 17773 0.11% 90 18483 0.11% 91 19912 0.12% 92 19215 0.11% 93 23618 0.14% 94 24176 0.14% 95 27337 0.16% 96 26409 0.16% 97 27632 0.16% 98 27650 0.16% 99 29561 0.18% 100 32942 0.20% 101 30436 0.18% 102 31941 0.19% 103 32991 0.20% 104 35050 0.21% 105 40137 0.24% 106 38419 0.23% 107 37421 0.22% 108 40218 0.24% 109 47670 0.28% 110 48793 0.29% 111 44195 0.26% 112 45189 0.27% 113 53132 0.32% 114 48166 0.29% 115 52272 0.31% 116 54594 0.32% 117 52447 0.31% 118 54810 0.33% 119 55803 0.33% 120 59883 0.36% 121 57501 0.34% 122 61424 0.37% 123 63886 0.38% 124 65728 0.39% 125 65119 0.39% 126 65873 0.39% 127 68582 0.41% 128 70035 0.42% 129 72255 0.43% 130 76240 0.45% 131 77891 0.46% 132 80880 0.48% 133 85194 0.51% 134 92055 0.55% 135 94926 0.56% 136 96102 0.57% 137 105232 0.63% 138 110044 0.65% 139 114997 0.68% 140 119658 0.71% 141 133068 0.79% 142 141249 0.84% 143 155063 0.92% 144 174254 1.04% 145 203498 1.21% 146 246685 1.47% 147 325858 1.94% 148 494748 2.94% 149 951399 5.66% 150 4199726 24.99% 151 6725402 40.02% 16805073 reads passed initial QC criterion=sequence-density sequence-density=0.87 sequence-density-rank=1 fanout-score=4.11 fanout-score-rank=18 prefix-density=1.21 prefix-fanout=2.9 sequence=CTGTCTCACGACG criterion=fanout-score sequence-density=0.12 sequence-density-rank=37 fanout-score=24.08 fanout-score-rank=1 prefix-density=2.77 prefix-fanout=1.0 sequence=GCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTG criterion=sequence-density sequence-density=2.60 sequence-density-rank=1 fanout-score=2.17 fanout-score-rank=30 prefix-density=2.70 prefix-fanout=2.1 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=227.52 fanout-score-rank=1 prefix-density=2.98 prefix-fanout=1.0 sequence=AAGAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTCGAGCACTCGACCGAAGATGTCTTGCTGCGGAGGAAACTGCAACTGCGGGTCATCCTGCAAGTGCGGCAGCGGCTGCAACGGCTGCAACATGTACCCTGAAGCCGAGGTCCAGACCTCCAGCCTCCTCGTCGTCGCCACCGCCGCCCACAAGGCGAGCTCCGGCGGGATGGAGATGGCCGCGGAGAACGGCGGCTGCGGCTGCAGCACCTGCAAG SRR7473328 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 13:34:13 Started mapping on | Dec 07 13:34:13 Finished on | Dec 07 13:46:38 Mapping speed, Million of reads per hour | 81.21 Number of input reads | 16805073 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 10682571 Uniquely mapped reads % | 63.57% Average mapped length | 291.51 Number of splices: Total | 9150960 Number of splices: Annotated (sjdb) | 8615424 Number of splices: GT/AG | 9030397 Number of splices: GC/AG | 105064 Number of splices: AT/AC | 4521 Number of splices: Non-canonical | 10978 Mismatch rate per base, % | 0.17% Deletion rate per base | 0.01% Deletion average length | 1.47 Insertion rate per base | 0.01% Insertion average length | 1.59 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 500358 % of reads mapped to multiple loci | 2.98% Number of reads mapped to too many loci | 84550 % of reads mapped to too many loci | 0.50% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 28.08% % of reads unmapped: other | 4.87% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5633645 5633645 5633645 N_multimapping 500358 500358 500358 N_noFeature 548960 10300344 684834 N_ambiguous 283671 1771 37892 UnstrandedReadsAssigned:9849940 PositiveStrandReadsAssigned:380456 NegativeStrandReadsAssigned:9959845 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=144 echo kmer=139 SRR7473328 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473328-trimmed-pair1.fastq SRR7473328-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,805,073 reads, 10,302,835 reads pseudoaligned [quant] estimated average fragment length: 243.842 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,223 rounds 52973 SRR7473328.ke.tsv 35125 SRR7473328.se.tsv 88098 total ==> SRR7473328.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 693.625 55.2607 9.36039 PNS24247 1044 801.158 0 0 PNS24249 1928 1685.16 73.3412 5.1134 PNS24246 1044 801.158 0 0 PNS24248 1044 801.158 0 0 PNS24244 1471 1228.16 144.398 13.8137 PNS24243 293 99.3554 1 1.18253 KQK14069 1603 1360.16 219.104 18.9262 KQK14071 474 245.867 0 0 ==> SRR7473328.se.tsv <== BRADI_1g14170v3 227 BRADI_1g53295v3 10 BRADI_1g59795v3 34 BRADI_1g07683v3 0 BRADI_1g00485v3 9 BRADI_1g20270v3 275 BRADI_1g74790v3 137 BRADI_1g09890v3 0 BRADI_1g77505v3 76 BRADI_1g48960v3 0 SRR7473328 completed mapping pipeline successfully