Starting /dee2/code/volunteer_pipeline.sh SRR7473343 current disk space = 1543103246336 free memory = 1600281912 SRR7473343 SRAfilesize a6979bfd4e02d4383d570e8f9a424cf5 SRR7473343.sra SRR7473343.sra file validated SRR7473343 is paired end SRR7473343 is conventional basespace SRR7473343 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473343_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.447 34.0 34.0 34.0 33.0 34.0 2 33.45075 34.0 34.0 34.0 33.0 34.0 3 33.44575 34.0 34.0 34.0 33.0 34.0 4 33.3445 34.0 34.0 34.0 33.0 34.0 5 33.47375 34.0 34.0 34.0 33.0 34.0 6 37.2145 38.0 38.0 38.0 36.0 38.0 7 37.5185 38.0 38.0 38.0 37.0 38.0 8 37.48775 38.0 38.0 38.0 38.0 38.0 9 37.52625 38.0 38.0 38.0 38.0 38.0 10-14 37.45845 38.0 38.0 38.0 37.8 38.0 15-19 37.54765 38.0 38.0 38.0 38.0 38.0 20-24 37.502250000000004 38.0 38.0 38.0 38.0 38.0 25-29 37.482600000000005 38.0 38.0 38.0 38.0 38.0 30-34 37.2706 38.0 38.0 38.0 37.2 38.0 35-39 37.4917 38.0 38.0 38.0 38.0 38.0 40-44 36.89975 38.0 38.0 38.0 35.8 38.0 45-49 37.2888 38.0 38.0 38.0 37.0 38.0 50-54 37.12095000000001 38.0 38.0 38.0 36.4 38.0 55-59 36.985749999999996 38.0 38.0 38.0 36.2 38.0 60-64 37.177249999999994 38.0 38.0 38.0 37.0 38.0 65-69 37.14444999999999 38.0 38.0 38.0 36.6 38.0 70-74 36.64015 38.0 38.0 38.0 35.6 38.0 75-79 34.23395000000001 38.0 38.0 38.0 19.4 38.0 80-84 33.958499999999994 38.0 38.0 38.0 15.0 38.0 85-89 33.96435 38.0 38.0 38.0 15.0 38.0 90-94 33.92975 38.0 38.0 38.0 14.8 38.0 95-99 33.85125000000001 38.0 38.0 38.0 14.0 38.0 100-104 33.716550000000005 38.0 38.0 38.0 13.6 38.0 105-109 33.52475 38.0 37.8 38.0 13.0 38.0 110-114 33.50735 38.0 37.8 38.0 13.0 38.0 115-119 33.41175 38.0 37.4 38.0 13.0 38.0 120-124 33.2269 38.0 36.4 38.0 2.0 38.0 125-129 32.975249999999996 38.0 36.0 38.0 2.0 38.0 130-134 32.82795 38.0 35.8 38.0 2.0 38.0 135-139 32.5708 38.0 35.0 38.0 2.0 38.0 140-144 32.44085 38.0 35.0 38.0 2.0 38.0 145-149 31.9594 38.0 34.8 38.0 2.0 38.0 150-151 28.796 36.0 18.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 1.0 12 5.0 13 4.0 14 24.0 15 16.0 16 24.0 17 31.0 18 93.0 19 167.0 20 9.0 21 12.0 22 2.0 23 8.0 24 8.0 25 10.0 26 13.0 27 12.0 28 15.0 29 22.0 30 20.0 31 36.0 32 40.0 33 65.0 34 72.0 35 139.0 36 362.0 37 2787.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.625000000000004 11.85 14.875 42.65 2 21.076345431789736 20.700876095118897 31.639549436795996 26.583229036295368 3 20.25 16.5 28.375 34.875 4 24.775 23.025000000000002 17.849999999999998 34.35 5 33.324999999999996 27.325 21.125 18.224999999999998 6 26.325 31.85 23.200000000000003 18.625 7 14.774999999999999 29.575000000000003 38.675 16.975 8 19.575 28.425 27.375 24.625 9 25.025 21.325 30.15 23.5 10-14 21.48 27.794999999999998 23.265 27.46 15-19 21.615000000000002 24.58 26.185000000000002 27.62 20-24 21.375 27.85 24.67 26.105 25-29 21.535 25.615 26.015 26.834999999999997 30-34 21.355 25.215 24.86 28.57 35-39 19.6 29.385 27.625 23.39 40-44 20.86 25.0 27.235 26.905 45-49 23.43 24.345 27.665 24.560000000000002 50-54 21.895 22.99 25.715 29.4 55-59 21.665 23.47 29.25 25.615 60-64 21.4 25.45 28.21 24.94 65-69 19.78 32.54 23.65 24.03 70-74 20.169999999999998 32.629999999999995 23.794999999999998 23.405 75-79 20.5 31.424999999999997 23.44 24.635 80-84 20.47 30.064999999999998 25.290000000000003 24.175 85-89 21.485000000000003 27.915 25.285000000000004 25.314999999999998 90-94 21.59 26.14 26.490000000000002 25.779999999999998 95-99 21.61 26.495 26.674999999999997 25.22 100-104 20.77 30.380000000000003 24.335 24.515 105-109 20.895 31.685000000000002 23.1 24.32 110-114 21.531076553827692 31.1715585779289 22.846142307115354 24.451222561128056 115-119 21.740000000000002 30.915 23.07 24.275 120-124 21.325 29.975 23.655 25.045 125-129 22.405 29.04 23.865 24.69 130-134 21.525 29.759999999999998 24.315 24.4 135-139 21.475 29.475 24.355 24.695 140-144 21.884999999999998 29.654999999999998 23.465 24.995 145-149 21.667751138695632 28.820261274338055 24.385604885129386 25.12638270183693 150-151 21.1625 29.525000000000002 24.3125 25.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 1.0 3 0.0 4 1.5 5 1.5 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 1.0 26 3.0 27 7.0 28 8.5 29 8.5 30 14.5 31 27.5 32 45.0 33 55.0 34 73.0 35 99.0 36 108.5 37 129.5 38 144.0 39 153.5 40 147.0 41 129.5 42 138.0 43 136.5 44 137.5 45 139.5 46 139.5 47 153.0 48 148.5 49 123.0 50 126.0 51 136.0 52 135.0 53 149.5 54 181.0 55 170.5 56 123.5 57 106.0 58 93.5 59 75.0 60 58.0 61 48.5 62 50.0 63 45.0 64 33.0 65 24.5 66 23.0 67 25.0 68 24.0 69 21.0 70 15.5 71 12.0 72 10.5 73 10.5 74 8.0 75 5.0 76 4.0 77 2.5 78 1.5 79 1.0 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.105 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.95 #Duplication Level Percentage of deduplicated Percentage of total 1 94.43120905300775 79.27499999999999 2 3.7224538415723645 6.25 3 0.9231685527099465 2.325 4 0.2680166765932102 0.8999999999999999 5 0.1786777843954735 0.75 6 0.14889815366289458 0.75 7 0.08933889219773675 0.525 8 0.08933889219773675 0.6 9 0.05955926146515784 0.44999999999999996 >10 0.05955926146515784 1.375 >50 0.0 0.0 >100 0.02977963073257892 6.800000000000001 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATATAATCTCGTATGC 272 6.800000000000001 TruSeq Adapter, Index 9 (97% over 36bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATATAATCTCGTATGCC 43 1.075 TruSeq Adapter, Index 9 (97% over 35bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATATAAACTCGTATGCC 12 0.3 TruSeq Adapter, Index 9 (97% over 35bp) CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 9 0.22499999999999998 No Hit CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 9 0.22499999999999998 No Hit CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC 8 0.2 No Hit CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG 8 0.2 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 8 0.2 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 7 0.17500000000000002 No Hit CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG 7 0.17500000000000002 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 7 0.17500000000000002 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 6 0.15 No Hit GAAGAGCACACGTCTGAACTCCAGTCACGATATAATCTCGTATGCCGTCT 6 0.15 TruSeq Adapter, Index 9 (96% over 31bp) CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA 6 0.15 No Hit TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG 6 0.15 No Hit GCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTT 6 0.15 No Hit CCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCC 5 0.125 No Hit CTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCAT 5 0.125 No Hit CCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTGTCGG 5 0.125 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 5 0.125 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 5 0.125 No Hit CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.075 0.0 0.0 0.0 0.0 2 0.075 0.0 0.0 0.0 0.0 3 0.075 0.0 0.0 0.0 0.0 4 0.075 0.0 0.0 0.0 0.0 5 0.075 0.0 0.0 0.0 0.025 6 0.075 0.0 0.0 0.0 0.025 7 0.075 0.0 0.0 0.0 0.025 8 0.075 0.0 0.0 0.0 0.025 9 0.075 0.0 0.0 0.0 0.025 10-11 0.075 0.0 0.0 0.0 0.025 12-13 0.075 0.0 0.0 0.0 0.025 14-15 0.075 0.0 0.0 0.0 0.025 16-17 0.075 0.0 0.0 0.0 0.025 18-19 0.075 0.0 0.0 0.0 0.025 20-21 0.075 0.0 0.0 0.0 0.025 22-23 0.075 0.0 0.0 0.0 0.025 24-25 0.075 0.0 0.0 0.0 0.025 26-27 0.075 0.0 0.0 0.0 0.025 28-29 0.075 0.0 0.0 0.0 0.025 30-31 0.075 0.0 0.0 0.0 0.025 32-33 0.075 0.0 0.0 0.0 0.025 34-35 0.075 0.0 0.0 0.0 0.025 36-37 0.075 0.0 0.0 0.0 0.025 38-39 0.075 0.0 0.0 0.0 0.025 40-41 0.075 0.0 0.0 0.0 0.025 42-43 0.1 0.0 0.0 0.0 0.025 44-45 0.1 0.0 0.0 0.0 0.025 46-47 0.1 0.0 0.0 0.0 0.025 48-49 0.1 0.0 0.0 0.0 0.025 50-51 0.125 0.0 0.0 0.0 0.025 52-53 0.125 0.0 0.0 0.0 0.025 54-55 0.125 0.0 0.0 0.0 0.025 56-57 0.16249999999999998 0.0 0.0 0.0 0.025 58-59 0.175 0.0 0.0 0.0 0.025 60-61 0.175 0.0 0.0 0.0 0.025 62-63 0.2 0.0 0.0 0.0 0.025 64-65 0.25 0.0 0.0 0.0 0.025 66-67 0.275 0.0 0.0 0.0 0.025 68-69 0.2875 0.0 0.0 0.0 0.025 70-71 0.325 0.0 0.0 0.0 0.025 72-73 0.3375 0.0 0.0 0.0 0.025 74-75 0.425 0.0 0.0 0.0 0.025 76-77 0.55 0.0 0.0 0.0 0.025 78-79 0.65 0.0 0.0 0.0 0.025 80-81 0.675 0.0 0.0 0.0 0.025 82-83 0.7875000000000001 0.0 0.0 0.0 0.025 84-85 0.8999999999999999 0.0 0.0 0.0 0.025 86-87 0.975 0.0 0.0 0.0 0.025 88-89 1.0875 0.0 0.0 0.0 0.025 90-91 1.275 0.0 0.0 0.0 0.025 92-93 1.6124999999999998 0.0 0.0 0.0 0.025 94-95 1.9249999999999998 0.0 0.0 0.0 0.025 96-97 2.25 0.0 0.0 0.0 0.025 98-99 2.5625 0.0 0.0 0.0 0.025 100-101 2.9 0.0 0.0 0.0 0.025 102-103 3.2 0.0 0.0 0.0 0.025 104-105 3.6875 0.0 0.0 0.0 0.025 106-107 4.225 0.0 0.0 0.0 0.025 108-109 4.875 0.0 0.0 0.0 0.025 110-111 5.6125 0.0 0.0 0.0 0.025 112-113 6.2625 0.0 0.0 0.0 0.025 114-115 6.7125 0.0 0.0 0.0 0.025 116-117 7.3625 0.0 0.0 0.0 0.025 118-119 7.800000000000001 0.0 0.0 0.0 0.025 120-121 8.3875 0.0 0.0 0.0 0.025 122-123 8.962499999999999 0.0 0.0 0.0 0.025 124-125 9.5625 0.0 0.0 0.0 0.025 126-127 10.4125 0.0 0.0 0.0 0.025 128-129 10.975 0.0 0.0 0.0 0.025 130-131 11.725000000000001 0.0 0.0 0.0 0.025 132-133 12.875 0.0 0.0 0.0 0.025 134-135 13.7125 0.0 0.0 0.0 0.025 136-137 14.3875 0.0 0.0 0.0 0.025 138-139 15.087499999999999 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCCTTC 10 0.006830828 145.0 2 ACATTTC 10 0.006830828 145.0 6 GATCGGA 105 2.9547027E-6 41.42857 1 TCGGAAG 105 2.9547027E-6 41.42857 3 CGGAAGA 105 2.9547027E-6 41.42857 4 ATCGGAA 105 2.9547027E-6 41.42857 2 GAAGAGC 110 4.0695504E-6 39.545456 6 GAGCACA 110 4.0695504E-6 39.545456 9 AGAGCAC 110 4.0695504E-6 39.545456 8 GGAAGAG 110 4.0695504E-6 39.545456 5 AAGAGCA 115 5.5244836E-6 37.826088 7 TGCCGTC 45 8.383813E-7 25.777777 45-49 TATGCCG 45 8.383813E-7 25.777777 45-49 ATGCCGT 45 8.383813E-7 25.777777 45-49 TTGAAAA 45 8.383813E-7 25.777777 60-64 GCCGTCT 45 8.383813E-7 25.777777 45-49 CGTCTTC 45 8.383813E-7 25.777777 50-54 GAAAAAA 50 2.0994885E-6 23.199999 60-64 GTCTTCT 50 2.0994885E-6 23.199999 50-54 TCGTATG 50 2.0994885E-6 23.199999 40-44 >>END_MODULE SRR7473343 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473343_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.97 34.0 33.0 34.0 32.0 34.0 2 33.08175 34.0 33.0 34.0 33.0 34.0 3 32.86775 34.0 33.0 34.0 33.0 34.0 4 32.75625 34.0 33.0 34.0 32.0 34.0 5 32.92475 34.0 33.0 34.0 33.0 34.0 6 36.77975 38.0 38.0 38.0 37.0 38.0 7 36.86325 38.0 38.0 38.0 36.0 38.0 8 36.7705 38.0 38.0 38.0 37.0 38.0 9 36.9265 38.0 38.0 38.0 37.0 38.0 10-14 36.79785 38.0 38.0 38.0 37.0 38.0 15-19 36.87445 38.0 38.0 38.0 37.0 38.0 20-24 36.9276 38.0 38.0 38.0 37.0 38.0 25-29 36.87545 38.0 38.0 38.0 37.0 38.0 30-34 36.82155 38.0 38.0 38.0 36.8 38.0 35-39 36.6134 38.0 38.0 38.0 36.2 38.0 40-44 36.7606 38.0 38.0 38.0 36.6 38.0 45-49 36.545700000000004 38.0 38.0 38.0 35.8 38.0 50-54 36.60405000000001 38.0 38.0 38.0 36.2 38.0 55-59 36.7826 38.0 38.0 38.0 36.6 38.0 60-64 36.967499999999994 38.0 38.0 38.0 37.0 38.0 65-69 35.826100000000004 38.0 38.0 38.0 31.0 38.0 70-74 33.891650000000006 38.0 38.0 38.0 14.6 38.0 75-79 33.869150000000005 38.0 38.0 38.0 14.0 38.0 80-84 33.73480000000001 38.0 38.0 38.0 2.0 38.0 85-89 33.66285 38.0 38.0 38.0 2.0 38.0 90-94 33.5449 38.0 38.0 38.0 2.0 38.0 95-99 33.573949999999996 38.0 38.0 38.0 2.0 38.0 100-104 33.441649999999996 38.0 38.0 38.0 2.0 38.0 105-109 33.23819999999999 38.0 37.6 38.0 2.0 38.0 110-114 32.969049999999996 38.0 36.2 38.0 2.0 38.0 115-119 32.60265 38.0 35.4 38.0 2.0 38.0 120-124 32.8647 38.0 36.0 38.0 2.0 38.0 125-129 32.782349999999994 38.0 35.8 38.0 2.0 38.0 130-134 32.37185 38.0 35.0 38.0 2.0 38.0 135-139 32.137950000000004 38.0 34.6 38.0 2.0 38.0 140-144 31.517149999999997 38.0 33.0 38.0 2.0 38.0 145-149 30.84395 38.0 32.4 38.0 2.0 38.0 150-151 26.716749999999998 34.5 16.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 5.0 4 2.0 5 1.0 6 2.0 7 1.0 8 2.0 9 3.0 10 6.0 11 45.0 12 19.0 13 10.0 14 4.0 15 11.0 16 33.0 17 218.0 18 20.0 19 8.0 20 9.0 21 3.0 22 8.0 23 7.0 24 9.0 25 15.0 26 10.0 27 12.0 28 12.0 29 27.0 30 32.0 31 36.0 32 46.0 33 61.0 34 94.0 35 159.0 36 441.0 37 2604.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.625000000000004 16.45 17.4 35.525 2 26.18272841051314 27.584480600750936 27.008760951188986 19.22403003754693 3 23.71056584877316 22.684026039058587 30.47070605908863 23.13470205307962 4 27.64838467317806 25.469571750563485 18.883045329326322 27.998998246932132 5 37.00550826239359 27.315973960941413 18.102153229844767 17.57636454682023 6 29.65 34.300000000000004 17.275 18.775 7 20.9 25.55 33.175 20.375 8 24.731182795698924 28.382095523880967 21.180295073768445 25.70642660665166 9 29.849999999999998 23.525 23.775 22.85 10-14 28.24694816890134 25.890534320592355 21.59795877526516 24.264558735241145 15-19 28.235647129425885 24.28985797159432 24.71994398879776 22.754550910182036 20-24 30.406081216243248 27.0754150830166 21.624324864972994 20.894178835767153 25-29 27.976190476190478 29.056622649059623 22.008803521408563 20.958383353341336 30-34 28.671433571678584 25.971298564928247 24.856242812140607 20.501025051252565 35-39 25.18503700740148 24.42988597719544 25.880176035207043 24.50490098019604 40-44 31.83295823955989 23.510877719429857 23.815953988497125 20.84021005251313 45-49 27.167225251363114 22.905307388324747 23.590615777099693 26.336851583212447 50-54 26.072821846553968 25.327598279483844 26.297889366810047 22.301690507152145 55-59 24.18225467640292 28.868660598179453 26.427928378513556 20.52115634690407 60-64 24.14569470155601 32.30099564717066 22.46460199129434 21.088707659978986 65-69 24.903639185062822 32.32717625269059 22.621014166291236 20.148170395955347 70-74 24.918721552543392 31.015855549442307 23.488220877307057 20.577202020707247 75-79 25.107510751075107 31.01810181018102 23.087308730873087 20.787078707870787 80-84 26.679337768218875 29.985494923223126 22.567898764567598 20.767268543990397 85-89 26.555 28.465 23.935000000000002 21.044999999999998 90-94 26.863431715857928 28.264132066033014 23.89194597298649 20.98049024512256 95-99 26.376318815940795 29.10145507275364 23.811190559527976 20.71103555177759 100-104 26.365 28.9 23.69 21.044999999999998 105-109 25.807903951975987 29.359679839919963 23.231615807903953 21.6008004002001 110-114 26.456298522414222 29.812171299774604 22.865013774104685 20.866516403706488 115-119 26.81765796462394 29.819111088841012 22.999448814952146 20.3637821315829 120-124 26.98023517638229 29.727295471603703 22.191643732799598 21.10082561921441 125-129 26.424999999999997 29.79 23.39 20.395 130-134 27.6 29.78 22.475 20.145 135-139 27.400000000000002 28.82 22.994999999999997 20.785 140-144 27.900000000000002 28.89 23.125 20.085 145-149 27.71 28.9 23.005 20.385 150-151 27.450000000000003 30.65 22.675 19.225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 1.0 21 1.5 22 1.0 23 1.0 24 1.0 25 1.5 26 2.5 27 4.5 28 7.0 29 11.0 30 20.0 31 21.5 32 25.5 33 34.0 34 55.0 35 82.0 36 94.0 37 83.5 38 83.5 39 112.5 40 140.0 41 138.0 42 134.0 43 150.5 44 138.0 45 125.5 46 138.0 47 147.5 48 152.5 49 158.5 50 153.0 51 136.0 52 137.0 53 159.5 54 170.0 55 164.5 56 140.0 57 119.0 58 114.5 59 91.5 60 74.5 61 70.0 62 68.0 63 54.5 64 34.5 65 29.0 66 28.5 67 35.0 68 32.5 69 23.0 70 18.5 71 17.5 72 16.5 73 14.5 74 11.0 75 5.5 76 3.0 77 2.5 78 1.5 79 2.5 80 2.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.15 4 0.17500000000000002 5 0.15 6 0.0 7 0.0 8 0.025 9 0.0 10-14 0.06 15-19 0.02 20-24 0.02 25-29 0.04 30-34 0.005 35-39 0.02 40-44 0.025 45-49 0.045 50-54 0.03 55-59 0.03 60-64 0.065 65-69 0.11499999999999999 70-74 0.034999999999999996 75-79 0.01 80-84 0.034999999999999996 85-89 0.0 90-94 0.05 95-99 0.005 100-104 0.0 105-109 0.05 110-114 0.17500000000000002 115-119 0.215 120-124 0.075 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.95 #Duplication Level Percentage of deduplicated Percentage of total 1 94.32018834608593 80.125 2 3.8552089464390815 6.550000000000001 3 1.088875809299588 2.775 4 0.3237198351971748 1.0999999999999999 5 0.11771630370806356 0.5 6 0.11771630370806356 0.6 7 0.0 0.0 8 0.08828722778104767 0.6 9 0.02942907592701589 0.22499999999999998 >10 0.02942907592701589 0.4 >50 0.0 0.0 >100 0.02942907592701589 7.124999999999999 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 285 7.124999999999999 Illumina Single End PCR Primer 1 (100% over 50bp) CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 16 0.4 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 9 0.22499999999999998 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 8 0.2 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 8 0.2 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 8 0.2 No Hit CCTCTTGTTAGCTATCTACGTACGTGTACGATGGCTCCCACAGTGATGTC 6 0.15 No Hit CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG 6 0.15 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 6 0.15 No Hit GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA 6 0.15 Illumina Single End PCR Primer 1 (100% over 50bp) GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 5 0.125 No Hit CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT 5 0.125 No Hit GCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGT 5 0.125 No Hit ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG 5 0.125 Illumina Single End PCR Primer 1 (97% over 49bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.075 0.0 0.0 0.0 0.0 2 0.075 0.0 0.0 0.0 0.0 3 0.075 0.0 0.0 0.0 0.0 4 0.075 0.0 0.0 0.0 0.0 5 0.075 0.0 0.0 0.0 0.0 6 0.075 0.0 0.0 0.0 0.0 7 0.075 0.0 0.0 0.0 0.0 8 0.075 0.0 0.0 0.0 0.0 9 0.075 0.0 0.0 0.0 0.0 10-11 0.075 0.0 0.0 0.0 0.0 12-13 0.075 0.0 0.0 0.0 0.0 14-15 0.075 0.0 0.0 0.0 0.0 16-17 0.075 0.0 0.0 0.0 0.0 18-19 0.075 0.0 0.0 0.0 0.0 20-21 0.075 0.0 0.0 0.0 0.0 22-23 0.075 0.0 0.0 0.0 0.0 24-25 0.075 0.0 0.0 0.0 0.0 26-27 0.075 0.0 0.0 0.0 0.0 28-29 0.075 0.0 0.0 0.0 0.0 30-31 0.075 0.0 0.0 0.0 0.0 32-33 0.075 0.0 0.0 0.0 0.0 34-35 0.075 0.0 0.0 0.0 0.0 36-37 0.075 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.16249999999999998 0.0 0.0 0.0 0.0 58-59 0.175 0.0 0.0 0.0 0.0 60-61 0.175 0.0 0.0 0.0 0.0 62-63 0.2 0.0 0.0 0.0 0.0 64-65 0.25 0.0 0.0 0.0 0.0 66-67 0.275 0.0 0.0 0.0 0.0 68-69 0.2875 0.0 0.0 0.0 0.0 70-71 0.325 0.0 0.0 0.0 0.0 72-73 0.3375 0.0 0.0 0.0 0.0 74-75 0.425 0.0 0.0 0.0 0.0 76-77 0.55 0.0 0.0 0.0 0.0 78-79 0.65 0.0 0.0 0.0 0.0 80-81 0.675 0.0 0.0 0.0 0.0 82-83 0.7875000000000001 0.0 0.0 0.0 0.0 84-85 0.8999999999999999 0.0 0.0 0.0 0.0 86-87 0.975 0.0 0.0 0.0 0.0 88-89 1.0875 0.0 0.0 0.0 0.0 90-91 1.275 0.0 0.0 0.0 0.0 92-93 1.6124999999999998 0.0 0.0 0.0 0.0 94-95 1.9249999999999998 0.0 0.0 0.0 0.0 96-97 2.25 0.0 0.0 0.0 0.0 98-99 2.5375 0.0 0.0 0.0 0.0 100-101 2.875 0.0 0.0 0.0 0.0 102-103 3.1875 0.0 0.0 0.0 0.0 104-105 3.6875 0.0 0.0 0.0 0.0 106-107 4.225 0.0 0.0 0.0 0.0 108-109 4.862500000000001 0.0 0.0 0.0 0.0 110-111 5.5875 0.0 0.0 0.0 0.0 112-113 6.1875 0.0 0.0 0.0 0.0 114-115 6.6375 0.0 0.0 0.0 0.0 116-117 7.2625 0.0 0.0 0.0 0.0 118-119 7.7125 0.0 0.0 0.0 0.0 120-121 8.3 0.0 0.0 0.0 0.0 122-123 8.8875 0.0 0.0 0.0 0.0 124-125 9.4875 0.0 0.0 0.0 0.0 126-127 10.375 0.0 0.0 0.0 0.0 128-129 10.95 0.0 0.0 0.0 0.0 130-131 11.7 0.0 0.0 0.0 0.0 132-133 12.8 0.0 0.0 0.0 0.0 134-135 13.625 0.0 0.0 0.0 0.0 136-137 14.274999999999999 0.0 0.0 0.0 0.0 138-139 14.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGTC 95 1.4826383E-6 45.789474 9 GATCGGA 100 2.1114429E-6 43.5 1 CGGAAGA 120 1.3968383E-7 42.291668 4 AAGAGCG 105 2.9547027E-6 41.42857 7 AGAGCGT 105 2.9547027E-6 41.42857 8 TCGGAAG 110 4.0695504E-6 39.545456 3 ATCGGAA 110 4.0695504E-6 39.545456 2 GAAGAGC 115 5.5244836E-6 37.826088 6 GGAAGAG 120 7.400906E-6 36.25 5 CCGTATC 40 9.990927E-6 25.375 45-49 CGTATCA 40 9.990927E-6 25.375 45-49 TATCATT 40 9.990927E-6 25.375 50-54 GCCGTAT 40 9.990927E-6 25.375 45-49 TCATTAA 40 9.990927E-6 25.375 50-54 GTCGCCG 45 2.4877938E-5 22.555553 40-44 TCGGTGG 45 2.4877938E-5 22.555553 35-39 CATTAAA 45 2.4877938E-5 22.555553 50-54 ATCATTA 45 2.4877938E-5 22.555553 50-54 CGCCGTA 45 2.4877938E-5 22.555553 45-49 GTATCAT 40 2.9585467E-4 21.75 50-54 >>END_MODULE Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra Read 987333 spots for SRR7473343.sra Written 987333 spots for SRR7473343.sra SRR ids: ['SRR7473343.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_d9fxukaj SRR7473343.sra spots: 19746660 blocks: [[1, 987333], [987334, 1974666], [1974667, 2961999], [2962000, 3949332], [3949333, 4936665], [4936666, 5923998], [5923999, 6911331], [6911332, 7898664], [7898665, 8885997], [8885998, 9873330], [9873331, 10860663], [10860664, 11847996], [11847997, 12835329], [12835330, 13822662], [13822663, 14809995], [14809996, 15797328], [15797329, 16784661], [16784662, 17771994], [17771995, 18759327], [18759328, 19746660]] SRR7473343 file size 6669794 SRR7473343 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473343 SRR7473343_1.fastq SRR7473343_2.fastq Input file: SRR7473343_1.fastq Paired file: SRR7473343_2.fastq trimmed: SRR7473343-trimmed-pair1.fastq, SRR7473343-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 14:14:09 2024 >> started Sat Dec 7 14:14:38 2024 >> done (28.713s) 19746660 read pairs processed; of these: 40056 ( 0.20%) short read pairs filtered out after trimming by size control 1484982 ( 7.52%) empty read pairs filtered out after trimming by size control 18221622 (92.28%) read pairs available; of these: 9151796 (50.22%) trimmed read pairs available after processing 9069826 (49.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 20 0.00% 19 25 0.00% 20 38 0.00% 21 32 0.00% 22 26 0.00% 23 27 0.00% 24 31 0.00% 25 21 0.00% 26 25 0.00% 27 40 0.00% 28 40 0.00% 29 52 0.00% 30 59 0.00% 31 91 0.00% 32 101 0.00% 33 93 0.00% 34 97 0.00% 35 118 0.00% 36 142 0.00% 37 151 0.00% 38 163 0.00% 39 193 0.00% 40 228 0.00% 41 259 0.00% 42 323 0.00% 43 338 0.00% 44 482 0.00% 45 702 0.00% 46 832 0.00% 47 851 0.00% 48 917 0.01% 49 1019 0.01% 50 1011 0.01% 51 1078 0.01% 52 1156 0.01% 53 1143 0.01% 54 1229 0.01% 55 1291 0.01% 56 1418 0.01% 57 1462 0.01% 58 1683 0.01% 59 1602 0.01% 60 1825 0.01% 61 2017 0.01% 62 2143 0.01% 63 2338 0.01% 64 2816 0.02% 65 7196 0.04% 66 7480 0.04% 67 7329 0.04% 68 12227 0.07% 69 79539 0.44% 70 149260 0.82% 71 94601 0.52% 72 46960 0.26% 73 28188 0.15% 74 21475 0.12% 75 17741 0.10% 76 14329 0.08% 77 13033 0.07% 78 12193 0.07% 79 11648 0.06% 80 11636 0.06% 81 12151 0.07% 82 13365 0.07% 83 14207 0.08% 84 17144 0.09% 85 19268 0.11% 86 20011 0.11% 87 22223 0.12% 88 25228 0.14% 89 25868 0.14% 90 27516 0.15% 91 27307 0.15% 92 28104 0.15% 93 34011 0.19% 94 32485 0.18% 95 36346 0.20% 96 36798 0.20% 97 39147 0.21% 98 39163 0.21% 99 40022 0.22% 100 41897 0.23% 101 40902 0.22% 102 43090 0.24% 103 43008 0.24% 104 46029 0.25% 105 50306 0.28% 106 48567 0.27% 107 48861 0.27% 108 50968 0.28% 109 54824 0.30% 110 56721 0.31% 111 53889 0.30% 112 54348 0.30% 113 58300 0.32% 114 58491 0.32% 115 63006 0.35% 116 63775 0.35% 117 60969 0.33% 118 62167 0.34% 119 63636 0.35% 120 65568 0.36% 121 63907 0.35% 122 69178 0.38% 123 72389 0.40% 124 68289 0.37% 125 69818 0.38% 126 70986 0.39% 127 74677 0.41% 128 73996 0.41% 129 75108 0.41% 130 78321 0.43% 131 78581 0.43% 132 80724 0.44% 133 82369 0.45% 134 88197 0.48% 135 86896 0.48% 136 88025 0.48% 137 95599 0.52% 138 100033 0.55% 139 104301 0.57% 140 104358 0.57% 141 109090 0.60% 142 114641 0.63% 143 123050 0.68% 144 131873 0.72% 145 145183 0.80% 146 166705 0.91% 147 205950 1.13% 148 295288 1.62% 149 571512 3.14% 150 3456483 18.97% 151 9069826 49.78% 18221622 reads passed initial QC criterion=sequence-density sequence-density=0.50 sequence-density-rank=1 fanout-score=4.39 fanout-score-rank=16 prefix-density=1.53 prefix-fanout=1.4 sequence=CACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=69.43 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=6.6 sequence=CTTGATGAAAATGGTATTATAATTATATAGTTGATGTCTTTTGGTCACAAGATGACCAAATTACGCATCACAAGTACAACCCCACGTCAGAAAATGGTAGAAACTTCTATTGCTTATTACAAATTCACATCGAGCCATCCGGCATGCAGTACTGGAAAATAGCGAGTACATATACTCCATGGCATCGCATCCACATCAATGGATCGATCTGTAGGGTCATCTCCATATCTGTATGTATAAGTATACGTTGTATGTATAGGAGTTAACCGGATGAGAGGACTTAGAGCTCCCATGTGTCGAACTTGCCGGAGACGAAGTCGTAGTGGCCGCCCACG criterion=sequence-density sequence-density=2.00 sequence-density-rank=1 fanout-score=2.22 fanout-score-rank=31 prefix-density=2.10 prefix-fanout=2.1 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.17 sequence-density-rank=22 fanout-score=11.83 fanout-score-rank=1 prefix-density=1.97 prefix-fanout=1.0 sequence=ATGTCTGGGAAGCTGCCTGATGG SRR7473343 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 14:15:51 Started mapping on | Dec 07 14:15:51 Finished on | Dec 07 14:31:35 Mapping speed, Million of reads per hour | 69.49 Number of input reads | 18221622 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 11787005 Uniquely mapped reads % | 64.69% Average mapped length | 290.43 Number of splices: Total | 11543909 Number of splices: Annotated (sjdb) | 10734698 Number of splices: GT/AG | 11393813 Number of splices: GC/AG | 131886 Number of splices: AT/AC | 6978 Number of splices: Non-canonical | 11232 Mismatch rate per base, % | 0.11% Deletion rate per base | 0.01% Deletion average length | 1.43 Insertion rate per base | 0.01% Insertion average length | 1.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 172189 % of reads mapped to multiple loci | 0.94% Number of reads mapped to too many loci | 16953 % of reads mapped to too many loci | 0.09% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 32.58% % of reads unmapped: other | 1.69% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6272435 6272435 6272435 N_multimapping 172189 172189 172189 N_noFeature 436007 11358077 557315 N_ambiguous 366730 1627 59785 UnstrandedReadsAssigned:10984268 PositiveStrandReadsAssigned:427301 NegativeStrandReadsAssigned:11169905 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=141 echo kmer=137 SRR7473343 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473343-trimmed-pair1.fastq SRR7473343-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,221,622 reads, 11,317,371 reads pseudoaligned [quant] estimated average fragment length: 234.174 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,100 rounds 52973 SRR7473343.ke.tsv 35125 SRR7473343.se.tsv 88098 total ==> SRR7473343.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 703.29 0 0 PNS24247 1044 810.826 6.43021 0.788843 PNS24249 1928 1694.83 7.06797 0.414823 PNS24246 1044 810.826 6.43021 0.788843 PNS24248 1044 810.826 6.43021 0.788843 PNS24244 1471 1237.83 109.641 8.81065 PNS24243 293 104.167 0 0 KQK14069 1603 1369.83 4342.39 315.324 KQK14071 474 253.875 135.492 53.0867 ==> SRR7473343.se.tsv <== BRADI_1g14170v3 4964 BRADI_1g53295v3 23 BRADI_1g59795v3 433 BRADI_1g07683v3 0 BRADI_1g00485v3 59 BRADI_1g20270v3 1457 BRADI_1g74790v3 178 BRADI_1g09890v3 1 BRADI_1g77505v3 436 BRADI_1g48960v3 0 SRR7473343 completed mapping pipeline successfully