Starting /dee2/code/volunteer_pipeline.sh SRR7473349 current disk space = 1543014801408 free memory = 1593403992 SRR7473349 SRAfilesize a3246609e52f9b664a1e56b717f6b60a SRR7473349.sra SRR7473349.sra file validated SRR7473349 is paired end SRR7473349 is conventional basespace SRR7473349 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473349_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.51975 34.0 34.0 34.0 33.0 34.0 2 33.57575 34.0 34.0 34.0 33.0 34.0 3 33.56075 34.0 34.0 34.0 33.0 34.0 4 33.55725 34.0 34.0 34.0 33.0 34.0 5 33.53875 34.0 34.0 34.0 33.0 34.0 6 37.349 38.0 38.0 38.0 37.0 38.0 7 37.5795 38.0 38.0 38.0 38.0 38.0 8 37.57475 38.0 38.0 38.0 38.0 38.0 9 37.557 38.0 38.0 38.0 38.0 38.0 10-14 37.5529 38.0 38.0 38.0 38.0 38.0 15-19 37.6422 38.0 38.0 38.0 38.0 38.0 20-24 37.601800000000004 38.0 38.0 38.0 38.0 38.0 25-29 37.5882 38.0 38.0 38.0 38.0 38.0 30-34 37.39635 38.0 38.0 38.0 37.4 38.0 35-39 37.55305 38.0 38.0 38.0 38.0 38.0 40-44 37.20255000000001 38.0 38.0 38.0 37.2 38.0 45-49 37.40565 38.0 38.0 38.0 37.8 38.0 50-54 37.26875 38.0 38.0 38.0 37.2 38.0 55-59 37.254949999999994 38.0 38.0 38.0 37.0 38.0 60-64 37.3608 38.0 38.0 38.0 37.6 38.0 65-69 37.1794 38.0 38.0 38.0 36.8 38.0 70-74 37.2658 38.0 38.0 38.0 37.4 38.0 75-79 36.83845 38.0 38.0 38.0 37.0 38.0 80-84 36.73425 38.0 38.0 38.0 36.4 38.0 85-89 36.7793 38.0 38.0 38.0 36.8 38.0 90-94 36.6846 38.0 38.0 38.0 36.4 38.0 95-99 36.67335 38.0 38.0 38.0 36.0 38.0 100-104 36.5972 38.0 38.0 38.0 35.8 38.0 105-109 36.4088 38.0 38.0 38.0 35.2 38.0 110-114 36.34085 38.0 38.0 38.0 35.0 38.0 115-119 36.28485 38.0 38.0 38.0 35.0 38.0 120-124 36.122499999999995 38.0 38.0 38.0 34.4 38.0 125-129 35.8786 38.0 38.0 38.0 33.4 38.0 130-134 35.695949999999996 38.0 38.0 38.0 33.0 38.0 135-139 35.577400000000004 38.0 37.8 38.0 32.8 38.0 140-144 35.2961 38.0 37.2 38.0 31.6 38.0 145-149 34.75005 38.0 36.0 38.0 29.2 38.0 150-151 31.543125 36.5 32.0 38.0 14.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 1.0 10 1.0 11 0.0 12 2.0 13 2.0 14 1.0 15 5.0 16 4.0 17 4.0 18 23.0 19 24.0 20 1.0 21 5.0 22 3.0 23 4.0 24 9.0 25 9.0 26 11.0 27 14.0 28 16.0 29 16.0 30 38.0 31 28.0 32 47.0 33 46.0 34 91.0 35 141.0 36 345.0 37 3106.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.075 12.075 11.25 39.6 2 23.655913978494624 16.22905726431608 33.383345836459114 26.731682920730183 3 21.425 20.275000000000002 26.700000000000003 31.6 4 23.95 28.575 21.425 26.05 5 25.0 31.2 23.375 20.424999999999997 6 20.549999999999997 33.875 26.025 19.55 7 14.875 23.7 42.825 18.6 8 18.85 24.75 30.275000000000002 26.125 9 18.95 22.6 32.95 25.5 10-14 20.815 27.88 25.905 25.4 15-19 21.485000000000003 26.395000000000003 26.669999999999998 25.45 20-24 20.415 27.36 27.089999999999996 25.135 25-29 20.560000000000002 26.88 27.08 25.480000000000004 30-34 20.885 28.04 26.090000000000003 24.985 35-39 21.62 26.884999999999998 27.01 24.485 40-44 21.529999999999998 27.045 26.695 24.73 45-49 21.515 27.26 26.795 24.43 50-54 21.2 27.08 26.755000000000003 24.965 55-59 20.9 26.395000000000003 27.134999999999998 25.569999999999997 60-64 20.599999999999998 26.63 27.565 25.205 65-69 20.515 27.63 26.915 24.94 70-74 21.16 28.444999999999997 25.52 24.875 75-79 20.695 28.28 25.924999999999997 25.1 80-84 21.04 27.944999999999997 25.6 25.415 85-89 21.990000000000002 26.69 25.61 25.71 90-94 21.755 26.435 26.484999999999996 25.324999999999996 95-99 21.9 27.01 26.13 24.959999999999997 100-104 20.96 27.805000000000003 25.929999999999996 25.305 105-109 21.845 27.505000000000003 25.635 25.014999999999997 110-114 21.806090304515227 27.92639631981599 24.781239061953098 25.486274313715683 115-119 21.535 28.415000000000003 24.91 25.14 120-124 21.45 27.495000000000005 24.995 26.06 125-129 22.33 26.810000000000002 25.465 25.395 130-134 22.755 27.474999999999998 25.180000000000003 24.59 135-139 22.355 26.995 25.264999999999997 25.385 140-144 23.415 26.215 24.84 25.53 145-149 23.322987585102123 26.677012414897877 25.26531838205847 24.73468161794153 150-151 23.525 25.924999999999997 24.8125 25.7375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.5 20 1.5 21 1.5 22 1.5 23 1.0 24 1.5 25 3.5 26 4.0 27 3.5 28 9.0 29 16.0 30 27.0 31 36.0 32 46.5 33 66.0 34 75.0 35 84.0 36 103.0 37 121.5 38 138.0 39 138.0 40 148.5 41 162.0 42 145.0 43 131.5 44 150.0 45 166.0 46 162.0 47 153.5 48 140.0 49 131.5 50 134.0 51 132.0 52 153.5 53 189.5 54 181.0 55 166.0 56 136.5 57 108.5 58 89.0 59 75.0 60 58.0 61 38.5 62 39.5 63 28.5 64 16.5 65 14.0 66 13.5 67 9.5 68 8.0 69 9.0 70 5.0 71 1.5 72 4.5 73 6.0 74 4.5 75 4.0 76 2.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.12 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.425 #Duplication Level Percentage of deduplicated Percentage of total 1 91.49551634365056 79.07499999999999 2 5.698582586057275 9.85 3 1.3306334972519527 3.45 4 0.6363899334683252 2.1999999999999997 5 0.37604859704946486 1.625 6 0.17356089094590688 0.8999999999999999 7 0.08678044547295344 0.525 8 0.02892681515765114 0.2 9 0.05785363031530228 0.44999999999999996 >10 0.11570726063060456 1.725 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC 38 0.95 TruSeq Adapter, Index 7 (97% over 36bp) GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 11 0.27499999999999997 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 10 0.25 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 10 0.25 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 9 0.22499999999999998 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 9 0.22499999999999998 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 8 0.2 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC 7 0.17500000000000002 TruSeq Adapter, Index 7 (97% over 35bp) GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 7 0.17500000000000002 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 7 0.17500000000000002 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 6 0.15 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 6 0.15 No Hit GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG 6 0.15 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 6 0.15 No Hit CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT 6 0.15 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 6 0.15 No Hit GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC 5 0.125 No Hit CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGT 5 0.125 No Hit CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC 5 0.125 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 5 0.125 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 5 0.125 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 5 0.125 No Hit GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC 5 0.125 No Hit CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT 5 0.125 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 5 0.125 No Hit CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT 5 0.125 No Hit GCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTT 5 0.125 No Hit CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCA 5 0.125 No Hit CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.1 0.0 0.0 0.0 0.0 2 0.1 0.0 0.0 0.0 0.0 3 0.1 0.0 0.0 0.0 0.0 4 0.1 0.0 0.0 0.0 0.0 5 0.1 0.0 0.0 0.0 0.0 6 0.1 0.0 0.0 0.0 0.0 7 0.1 0.0 0.0 0.0 0.0 8 0.1 0.0 0.0 0.0 0.0 9 0.1 0.0 0.0 0.0 0.0 10-11 0.1 0.0 0.0 0.0 0.0 12-13 0.1 0.0 0.0 0.0 0.0 14-15 0.1 0.0 0.0 0.0 0.0 16-17 0.1 0.0 0.0 0.0 0.0 18-19 0.1 0.0 0.0 0.0 0.0 20-21 0.1 0.0 0.0 0.0 0.0 22-23 0.1 0.0 0.0 0.0 0.0 24-25 0.1 0.0 0.0 0.0 0.0 26-27 0.1 0.0 0.0 0.0 0.0 28-29 0.1 0.0 0.0 0.0 0.0 30-31 0.1 0.0 0.0 0.0 0.0 32-33 0.1 0.0 0.0 0.0 0.0 34-35 0.1 0.0 0.0 0.0 0.0 36-37 0.1 0.0 0.0 0.0 0.0 38-39 0.1 0.0 0.0 0.0 0.0 40-41 0.1 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1125 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.2375 0.0 0.0 0.0 0.0 74-75 0.3125 0.0 0.0 0.0 0.0 76-77 0.3625 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.525 0.0 0.0 0.0 0.0 82-83 0.5875 0.0 0.0 0.0 0.0 84-85 0.65 0.0 0.0 0.0 0.0 86-87 0.7625 0.0 0.0 0.0 0.0 88-89 0.85 0.0 0.0 0.0 0.0 90-91 1.1124999999999998 0.0 0.0 0.0 0.0 92-93 1.3125 0.0 0.0 0.0 0.0 94-95 1.7 0.0 0.0 0.0 0.0 96-97 1.9749999999999999 0.0 0.0 0.0 0.0 98-99 2.6375 0.0 0.0 0.0 0.0 100-101 3.0625 0.0 0.0 0.0 0.0 102-103 3.5875000000000004 0.0 0.0 0.0 0.0 104-105 4.05 0.0 0.0 0.0 0.0 106-107 4.625 0.0 0.0 0.0 0.0 108-109 5.25 0.0 0.0 0.0 0.0 110-111 5.875 0.0 0.0 0.0 0.0 112-113 6.2875 0.0 0.0 0.0 0.0 114-115 6.85 0.0 0.0 0.0 0.0 116-117 7.5375 0.0 0.0 0.0 0.0 118-119 8.0875 0.0 0.0 0.0 0.0 120-121 8.85 0.0 0.0 0.0 0.0 122-123 9.4625 0.0 0.0 0.0 0.0 124-125 9.975000000000001 0.0 0.0 0.0 0.0 126-127 10.662500000000001 0.0 0.0 0.0 0.0 128-129 11.325 0.0 0.0 0.0 0.0 130-131 12.0875 0.0 0.0 0.0 0.0 132-133 12.6 0.0 0.0 0.0 0.0 134-135 13.575 0.0 0.0 0.0 0.0 136-137 14.524999999999999 0.0 0.0 0.0 0.0 138-139 15.399999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGCCC 10 0.006830828 145.0 145 CCCTCTT 10 0.006830828 145.0 1 TCTTTAC 10 0.006830828 145.0 5 CTTCTAA 10 0.006830828 145.0 5 CTGGGCA 20 0.00593511 29.0 125-129 TATGCCG 20 0.00593511 29.0 45-49 TTCCAGT 20 0.00593511 29.0 40-44 AAAAAAA 480 3.2937627E-5 5.4375 65-69 >>END_MODULE SRR7473349 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473349_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.0795 34.0 33.0 34.0 32.0 34.0 2 33.20375 34.0 33.0 34.0 33.0 34.0 3 33.08225 34.0 33.0 34.0 33.0 34.0 4 33.00125 34.0 33.0 34.0 33.0 34.0 5 33.102 34.0 33.0 34.0 33.0 34.0 6 37.1755 38.0 38.0 38.0 37.0 38.0 7 37.06175 38.0 38.0 38.0 37.0 38.0 8 37.17475 38.0 38.0 38.0 37.0 38.0 9 37.22825 38.0 38.0 38.0 37.0 38.0 10-14 37.13304999999999 38.0 38.0 38.0 37.2 38.0 15-19 37.18814999999999 38.0 38.0 38.0 37.4 38.0 20-24 37.23694999999999 38.0 38.0 38.0 37.8 38.0 25-29 37.224849999999996 38.0 38.0 38.0 37.6 38.0 30-34 37.28765 38.0 38.0 38.0 38.0 38.0 35-39 37.0863 38.0 38.0 38.0 37.2 38.0 40-44 37.18485 38.0 38.0 38.0 37.4 38.0 45-49 37.16945 38.0 38.0 38.0 37.4 38.0 50-54 37.21995 38.0 38.0 38.0 37.6 38.0 55-59 37.2471 38.0 38.0 38.0 37.6 38.0 60-64 37.205799999999996 38.0 38.0 38.0 37.6 38.0 65-69 36.9072 38.0 38.0 38.0 36.8 38.0 70-74 36.61129999999999 38.0 38.0 38.0 36.8 38.0 75-79 36.63895 38.0 38.0 38.0 36.6 38.0 80-84 36.57315 38.0 38.0 38.0 36.0 38.0 85-89 36.48115 38.0 38.0 38.0 36.0 38.0 90-94 36.33755000000001 38.0 38.0 38.0 35.4 38.0 95-99 36.3408 38.0 38.0 38.0 35.0 38.0 100-104 36.197250000000004 38.0 38.0 38.0 34.6 38.0 105-109 36.08945 38.0 38.0 38.0 34.4 38.0 110-114 35.75055 38.0 38.0 38.0 33.4 38.0 115-119 35.376149999999996 38.0 37.4 38.0 30.8 38.0 120-124 35.659549999999996 38.0 38.0 38.0 33.0 38.0 125-129 35.4428 38.0 37.6 38.0 31.4 38.0 130-134 35.08105 38.0 36.2 38.0 30.0 38.0 135-139 34.84905 38.0 36.0 38.0 29.4 38.0 140-144 34.17415 38.0 35.2 38.0 25.8 38.0 145-149 33.4841 38.0 34.0 38.0 20.6 38.0 150-151 28.885875 35.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 2.0 4 1.0 5 0.0 6 0.0 7 0.0 8 2.0 9 1.0 10 1.0 11 9.0 12 2.0 13 0.0 14 5.0 15 5.0 16 8.0 17 40.0 18 8.0 19 1.0 20 5.0 21 8.0 22 4.0 23 6.0 24 9.0 25 15.0 26 19.0 27 20.0 28 20.0 29 25.0 30 34.0 31 43.0 32 46.0 33 69.0 34 110.0 35 191.0 36 450.0 37 2833.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.824999999999996 17.7 14.325 30.15 2 30.582645661415352 22.83070767691923 28.432108027006752 18.154538634658664 3 24.730913642052567 24.60575719649562 27.459324155193993 23.204005006257823 4 27.45490981963928 31.462925851703403 20.265531062124246 20.816633266533067 5 29.604604604604607 32.38238238238238 19.61961961961962 18.393393393393392 6 25.324999999999996 35.05 20.175 19.45 7 21.65 20.575 36.875 20.9 8 25.05 24.05 24.025 26.875 9 24.625 24.125 26.724999999999998 24.525 10-14 27.224999999999998 26.185000000000002 22.615 23.974999999999998 15-19 27.26 25.61 24.224999999999998 22.905 20-24 26.87 26.575 24.05 22.505 25-29 26.561328066403323 27.061353067653382 24.466223311165557 21.911095554777738 30-34 26.695 27.395000000000003 24.67 21.240000000000002 35-39 26.229999999999997 26.08 25.305 22.384999999999998 40-44 27.376368818440923 26.276313815690784 24.956247812390618 21.391069553477674 45-49 26.072607260726073 25.807580758075808 25.532553255325535 22.587258725872587 50-54 25.974999999999998 25.795 26.69 21.54 55-59 25.374999999999996 26.290000000000003 26.584999999999997 21.75 60-64 24.56745674567457 27.382738273827385 26.357635763576358 21.69216921692169 65-69 25.927963981991 26.858429214607305 25.6128064032016 21.6008004002001 70-74 25.224999999999998 27.465 25.52 21.790000000000003 75-79 25.275 27.200000000000003 25.855 21.67 80-84 26.07630381519076 27.2063603180159 25.646282314115705 21.071053552677636 85-89 25.145 27.310000000000002 26.040000000000003 21.505 90-94 26.036301815090756 26.93134656732837 26.061303065153258 20.97104855242762 95-99 26.07 27.52 25.430000000000003 20.979999999999997 100-104 26.290000000000003 27.87 25.25 20.59 105-109 26.201310065503275 28.34141707085354 24.90624531226561 20.55102755137757 110-114 26.068889556423354 27.816161009312108 25.08761389806749 21.027335536197057 115-119 26.35798757265985 28.517739025856887 25.400881940268587 19.723391461214675 120-124 26.45425899064673 28.15485419896964 25.06377232031211 20.327114490071523 125-129 26.119999999999997 28.23 25.72 19.93 130-134 26.505000000000003 27.400000000000002 25.45 20.645 135-139 26.745 27.375 25.735000000000003 20.145 140-144 27.455000000000002 27.51 25.335 19.7 145-149 28.01 27.435 24.54 20.015 150-151 27.700000000000003 27.6 24.975 19.725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 2.0 22 2.5 23 1.5 24 2.5 25 4.0 26 3.0 27 3.5 28 6.0 29 11.5 30 19.0 31 23.0 32 23.5 33 32.5 34 54.5 35 67.0 36 79.5 37 102.0 38 122.0 39 115.5 40 128.0 41 139.0 42 115.0 43 113.0 44 139.0 45 150.0 46 141.5 47 156.0 48 167.0 49 168.5 50 152.0 51 141.0 52 167.0 53 193.5 54 192.5 55 189.0 56 174.5 57 121.5 58 90.0 59 92.0 60 77.0 61 55.0 62 54.5 63 47.5 64 30.0 65 24.0 66 19.5 67 17.5 68 16.5 69 14.5 70 8.5 71 5.0 72 10.0 73 7.0 74 2.0 75 2.0 76 2.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.125 4 0.2 5 0.1 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.005 30-34 0.0 35-39 0.0 40-44 0.005 45-49 0.01 50-54 0.0 55-59 0.0 60-64 0.01 65-69 0.05 70-74 0.0 75-79 0.0 80-84 0.005 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.005 110-114 0.13 115-119 0.22 120-124 0.034999999999999996 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.05000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 91.63277164439279 78.85 2 5.520046484601976 9.5 3 1.3945380592678676 3.5999999999999996 4 0.6682161533991865 2.3 5 0.2905287623474724 1.25 6 0.08715862870424172 0.44999999999999996 7 0.08715862870424172 0.525 8 0.11621150493898895 0.8 9 0.058105752469494475 0.44999999999999996 >10 0.1452643811737362 2.275 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 47 1.175 Illumina Single End PCR Primer 1 (100% over 50bp) CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 14 0.35000000000000003 No Hit GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 10 0.25 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 10 0.25 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 10 0.25 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 9 0.22499999999999998 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 9 0.22499999999999998 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 8 0.2 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 8 0.2 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 8 0.2 No Hit CGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAG 8 0.2 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 7 0.17500000000000002 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 7 0.17500000000000002 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 7 0.17500000000000002 No Hit CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG 6 0.15 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 6 0.15 No Hit CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG 6 0.15 No Hit GGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGG 5 0.125 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 5 0.125 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 5 0.125 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 5 0.125 No Hit CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG 5 0.125 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 5 0.125 No Hit CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG 5 0.125 No Hit CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC 5 0.125 No Hit GGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGA 5 0.125 No Hit CGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.1 0.0 0.0 0.0 0.0 2 0.1 0.0 0.0 0.0 0.0 3 0.1 0.0 0.0 0.0 0.0 4 0.1 0.0 0.0 0.0 0.0 5 0.1 0.0 0.0 0.0 0.0 6 0.1 0.0 0.0 0.0 0.0 7 0.1 0.0 0.0 0.0 0.0 8 0.1 0.0 0.0 0.0 0.0 9 0.1 0.0 0.0 0.0 0.0 10-11 0.1 0.0 0.0 0.0 0.0 12-13 0.1 0.0 0.0 0.0 0.0 14-15 0.1 0.0 0.0 0.0 0.0 16-17 0.1 0.0 0.0 0.0 0.0 18-19 0.1 0.0 0.0 0.0 0.0 20-21 0.1 0.0 0.0 0.0 0.0 22-23 0.1 0.0 0.0 0.0 0.0 24-25 0.1 0.0 0.0 0.0 0.0 26-27 0.1 0.0 0.0 0.0 0.0 28-29 0.1 0.0 0.0 0.0 0.0 30-31 0.1 0.0 0.0 0.0 0.0 32-33 0.1 0.0 0.0 0.0 0.0 34-35 0.1 0.0 0.0 0.0 0.0 36-37 0.1 0.0 0.0 0.0 0.0 38-39 0.1 0.0 0.0 0.0 0.0 40-41 0.1 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1125 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.2375 0.0 0.0 0.0 0.0 74-75 0.3125 0.0 0.0 0.0 0.0 76-77 0.3625 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.525 0.0 0.0 0.0 0.0 82-83 0.5875 0.0 0.0 0.0 0.0 84-85 0.6625 0.0 0.0 0.0 0.0 86-87 0.7875000000000001 0.0 0.0 0.0 0.0 88-89 0.875 0.0 0.0 0.0 0.0 90-91 1.1375000000000002 0.0 0.0 0.0 0.0 92-93 1.3375 0.0 0.0 0.0 0.0 94-95 1.725 0.0 0.0 0.0 0.0 96-97 2.0 0.0 0.0 0.0 0.0 98-99 2.65 0.0 0.0 0.0 0.0 100-101 3.0625 0.0 0.0 0.0 0.0 102-103 3.5375 0.0 0.0 0.0 0.0 104-105 3.95 0.0 0.0 0.0 0.0 106-107 4.5125 0.0 0.0 0.0 0.0 108-109 5.0875 0.0 0.0 0.0 0.0 110-111 5.6875 0.0 0.0 0.0 0.0 112-113 6.074999999999999 0.0 0.0 0.0 0.0 114-115 6.6375 0.0 0.0 0.0 0.0 116-117 7.4 0.0 0.0 0.0 0.0 118-119 7.9375 0.0 0.0 0.0 0.0 120-121 8.7 0.0 0.0 0.0 0.0 122-123 9.2875 0.0 0.0 0.0 0.0 124-125 9.7875 0.0 0.0 0.0 0.0 126-127 10.462499999999999 0.0 0.0 0.0 0.0 128-129 11.1 0.0 0.0 0.0 0.0 130-131 11.8375 0.0 0.0 0.0 0.0 132-133 12.337499999999999 0.0 0.0 0.0 0.0 134-135 13.2625 0.0 0.0 0.0 0.0 136-137 14.175 0.0 0.0 0.0 0.0 138-139 15.024999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATC 20 0.00593511 29.0 45-49 CGTATCA 20 0.00593511 29.0 45-49 TATCATT 20 0.00593511 29.0 50-54 ATCATTA 20 0.00593511 29.0 50-54 GCCGTAT 20 0.00593511 29.0 45-49 CGCCGTA 20 0.00593511 29.0 45-49 TTAAAAA 20 0.00593511 29.0 55-59 AAAAAAA 355 3.0067895E-9 8.169014 60-64 >>END_MODULE Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921772 spots for SRR7473349.sra Written 921772 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra Read 921759 spots for SRR7473349.sra Written 921759 spots for SRR7473349.sra SRR ids: ['SRR7473349.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_u2akyu05 SRR7473349.sra spots: 18435193 blocks: [[1, 921759], [921760, 1843518], [1843519, 2765277], [2765278, 3687036], [3687037, 4608795], [4608796, 5530554], [5530555, 6452313], [6452314, 7374072], [7374073, 8295831], [8295832, 9217590], [9217591, 10139349], [10139350, 11061108], [11061109, 11982867], [11982868, 12904626], [12904627, 13826385], [13826386, 14748144], [14748145, 15669903], [15669904, 16591662], [16591663, 17513421], [17513422, 18435193]] SRR7473349 file size 6225381 SRR7473349 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473349 SRR7473349_1.fastq SRR7473349_2.fastq Input file: SRR7473349_1.fastq Paired file: SRR7473349_2.fastq trimmed: SRR7473349-trimmed-pair1.fastq, SRR7473349-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 14:31:19 2024 >> started Sat Dec 7 14:31:39 2024 >> done (20.723s) 18435193 read pairs processed; of these: 23987 ( 0.13%) short read pairs filtered out after trimming by size control 243953 ( 1.32%) empty read pairs filtered out after trimming by size control 18167253 (98.55%) read pairs available; of these: 8718980 (47.99%) trimmed read pairs available after processing 9448273 (52.01%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 15 0.00% 20 10 0.00% 21 12 0.00% 22 16 0.00% 23 13 0.00% 24 17 0.00% 25 17 0.00% 26 24 0.00% 27 41 0.00% 28 24 0.00% 29 46 0.00% 30 38 0.00% 31 56 0.00% 32 41 0.00% 33 45 0.00% 34 37 0.00% 35 67 0.00% 36 44 0.00% 37 64 0.00% 38 82 0.00% 39 105 0.00% 40 124 0.00% 41 146 0.00% 42 137 0.00% 43 128 0.00% 44 134 0.00% 45 188 0.00% 46 189 0.00% 47 240 0.00% 48 293 0.00% 49 297 0.00% 50 393 0.00% 51 407 0.00% 52 492 0.00% 53 493 0.00% 54 493 0.00% 55 486 0.00% 56 513 0.00% 57 612 0.00% 58 713 0.00% 59 819 0.00% 60 890 0.00% 61 1124 0.01% 62 1285 0.01% 63 1397 0.01% 64 1455 0.01% 65 1768 0.01% 66 2030 0.01% 67 2648 0.01% 68 4535 0.02% 69 25661 0.14% 70 33204 0.18% 71 9732 0.05% 72 6277 0.03% 73 6070 0.03% 74 5974 0.03% 75 5827 0.03% 76 5712 0.03% 77 6358 0.03% 78 6688 0.04% 79 7744 0.04% 80 8292 0.05% 81 9172 0.05% 82 10269 0.06% 83 11967 0.07% 84 15752 0.09% 85 16582 0.09% 86 17443 0.10% 87 18689 0.10% 88 20842 0.11% 89 21539 0.12% 90 23328 0.13% 91 24095 0.13% 92 25186 0.14% 93 29883 0.16% 94 30540 0.17% 95 34402 0.19% 96 34006 0.19% 97 35198 0.19% 98 33705 0.19% 99 35146 0.19% 100 39054 0.21% 101 37464 0.21% 102 40537 0.22% 103 41672 0.23% 104 45124 0.25% 105 49644 0.27% 106 48140 0.26% 107 47793 0.26% 108 50823 0.28% 109 56416 0.31% 110 58551 0.32% 111 53108 0.29% 112 55745 0.31% 113 64511 0.36% 114 60974 0.34% 115 66227 0.36% 116 67940 0.37% 117 65050 0.36% 118 65588 0.36% 119 65538 0.36% 120 67890 0.37% 121 65490 0.36% 122 71759 0.39% 123 76058 0.42% 124 76610 0.42% 125 77386 0.43% 126 78074 0.43% 127 79850 0.44% 128 78351 0.43% 129 79263 0.44% 130 81732 0.45% 131 83304 0.46% 132 85002 0.47% 133 87927 0.48% 134 93840 0.52% 135 95308 0.52% 136 95252 0.52% 137 103113 0.57% 138 104611 0.58% 139 106372 0.59% 140 106075 0.58% 141 114569 0.63% 142 117322 0.65% 143 124574 0.69% 144 135523 0.75% 145 149604 0.82% 146 170734 0.94% 147 207967 1.14% 148 286429 1.58% 149 542527 2.99% 150 3396027 18.69% 151 9448273 52.01% 18167253 reads passed initial QC criterion=sequence-density sequence-density=0.66 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=41 prefix-density=0.64 prefix-fanout=2.0 sequence=TTCAAATGTACA criterion=fanout-score sequence-density=0.01 sequence-density-rank=42 fanout-score=32.74 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=5.9 sequence=TTTTTTTTTCGCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGCCGAGCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGC criterion=sequence-density sequence-density=0.60 sequence-density-rank=1 fanout-score=3.15 fanout-score-rank=22 prefix-density=1.89 prefix-fanout=1.0 sequence=GGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTG criterion=fanout-score sequence-density=0.04 sequence-density-rank=31 fanout-score=23.97 fanout-score-rank=1 prefix-density=1.02 prefix-fanout=1.0 sequence=CGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG SRR7473349 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 14:33:12 Started mapping on | Dec 07 14:33:12 Finished on | Dec 07 14:55:39 Mapping speed, Million of reads per hour | 48.55 Number of input reads | 18167253 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 10015736 Uniquely mapped reads % | 55.13% Average mapped length | 289.23 Number of splices: Total | 7231562 Number of splices: Annotated (sjdb) | 6661657 Number of splices: GT/AG | 7135098 Number of splices: GC/AG | 85108 Number of splices: AT/AC | 3643 Number of splices: Non-canonical | 7713 Mismatch rate per base, % | 0.11% Deletion rate per base | 0.01% Deletion average length | 1.43 Insertion rate per base | 0.01% Insertion average length | 1.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 150072 % of reads mapped to multiple loci | 0.83% Number of reads mapped to too many loci | 11173 % of reads mapped to too many loci | 0.06% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 42.40% % of reads unmapped: other | 1.58% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8009659 8009659 8009659 N_multimapping 150072 150072 150072 N_noFeature 358089 9648386 441456 N_ambiguous 326617 4795 43782 UnstrandedReadsAssigned:9331030 PositiveStrandReadsAssigned:362555 NegativeStrandReadsAssigned:9530498 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=144 echo kmer=139 SRR7473349 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473349-trimmed-pair1.fastq SRR7473349-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,167,253 reads, 9,692,049 reads pseudoaligned [quant] estimated average fragment length: 218.679 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,036 rounds 52973 SRR7473349.ke.tsv 35125 SRR7473349.se.tsv 88098 total ==> SRR7473349.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 718.647 0 0 PNS24247 1044 826.321 1.51481 0.235219 PNS24249 1928 1710.32 3.7425 0.280767 PNS24246 1044 826.321 1.51481 0.235219 PNS24248 1044 826.321 1.51481 0.235219 PNS24244 1471 1253.32 118.713 12.1534 PNS24243 293 109.719 0 0 KQK14069 1603 1385.32 2226.55 206.226 KQK14071 474 266.941 58.2288 27.9888 ==> SRR7473349.se.tsv <== BRADI_1g14170v3 2523 BRADI_1g53295v3 18 BRADI_1g59795v3 403 BRADI_1g07683v3 0 BRADI_1g00485v3 33 BRADI_1g20270v3 1675 BRADI_1g74790v3 77 BRADI_1g09890v3 1 BRADI_1g77505v3 332 BRADI_1g48960v3 0 SRR7473349 completed mapping pipeline successfully