Starting /dee2/code/volunteer_pipeline.sh SRR7473350 current disk space = 1542992158720 free memory = 1592760568 SRR7473350 SRAfilesize 0c024c3f2bd9da54c2f212e11b532906 SRR7473350.sra SRR7473350.sra file validated SRR7473350 is paired end SRR7473350 is conventional basespace SRR7473350 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473350_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.51175 34.0 34.0 34.0 33.0 34.0 2 33.5305 34.0 34.0 34.0 33.0 34.0 3 33.57725 34.0 34.0 34.0 33.0 34.0 4 33.59825 34.0 34.0 34.0 33.0 34.0 5 33.541 34.0 34.0 34.0 33.0 34.0 6 37.39875 38.0 38.0 38.0 37.0 38.0 7 37.5965 38.0 38.0 38.0 38.0 38.0 8 37.64275 38.0 38.0 38.0 38.0 38.0 9 37.66975 38.0 38.0 38.0 38.0 38.0 10-14 37.57340000000001 38.0 38.0 38.0 38.0 38.0 15-19 37.6586 38.0 38.0 38.0 38.0 38.0 20-24 37.68705 38.0 38.0 38.0 38.0 38.0 25-29 37.60000000000001 38.0 38.0 38.0 38.0 38.0 30-34 37.43955 38.0 38.0 38.0 37.4 38.0 35-39 37.62605 38.0 38.0 38.0 38.0 38.0 40-44 37.306349999999995 38.0 38.0 38.0 37.2 38.0 45-49 37.404999999999994 38.0 38.0 38.0 37.6 38.0 50-54 37.36175 38.0 38.0 38.0 37.2 38.0 55-59 37.264300000000006 38.0 38.0 38.0 36.6 38.0 60-64 37.42895 38.0 38.0 38.0 37.6 38.0 65-69 37.22044999999999 38.0 38.0 38.0 36.8 38.0 70-74 37.3699 38.0 38.0 38.0 37.2 38.0 75-79 37.0856 38.0 38.0 38.0 37.0 38.0 80-84 36.9704 38.0 38.0 38.0 36.4 38.0 85-89 37.0226 38.0 38.0 38.0 36.8 38.0 90-94 36.93045000000001 38.0 38.0 38.0 36.0 38.0 95-99 36.86225 38.0 38.0 38.0 36.0 38.0 100-104 36.77425 38.0 38.0 38.0 35.8 38.0 105-109 36.54625 38.0 38.0 38.0 35.0 38.0 110-114 36.529700000000005 38.0 38.0 38.0 35.0 38.0 115-119 36.435700000000004 38.0 38.0 38.0 34.6 38.0 120-124 36.277750000000005 38.0 38.0 38.0 34.0 38.0 125-129 35.993649999999995 38.0 38.0 38.0 33.6 38.0 130-134 35.7633 38.0 37.6 38.0 32.6 38.0 135-139 35.5839 38.0 36.8 38.0 32.2 38.0 140-144 35.3678 38.0 36.0 38.0 31.6 38.0 145-149 34.86705 38.0 35.8 38.0 29.8 38.0 150-151 31.530875 36.5 31.5 38.0 15.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 0.0 15 1.0 16 3.0 17 6.0 18 7.0 19 22.0 20 4.0 21 5.0 22 5.0 23 4.0 24 2.0 25 7.0 26 14.0 27 12.0 28 13.0 29 15.0 30 21.0 31 23.0 32 52.0 33 67.0 34 101.0 35 179.0 36 444.0 37 2991.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.475 11.475 9.725 42.325 2 24.88744372186093 14.732366183091546 31.6408204102051 28.73936968484242 3 21.7 17.8 24.2 36.3 4 26.3 25.75 19.5 28.449999999999996 5 28.825 28.475 21.475 21.224999999999998 6 21.775 30.75 25.124999999999996 22.35 7 16.675 20.75 40.9 21.675 8 20.875 21.475 27.800000000000004 29.849999999999998 9 18.224999999999998 21.15 32.824999999999996 27.800000000000004 10-14 22.485 24.0 24.7 28.815 15-19 22.770000000000003 24.279999999999998 24.83 28.12 20-24 23.05 24.169999999999998 25.169999999999998 27.61 25-29 22.105 24.645 25.130000000000003 28.12 30-34 22.535 24.205 24.77 28.49 35-39 22.884999999999998 23.76 25.955000000000002 27.400000000000002 40-44 23.315 23.485 25.05 28.15 45-49 23.31 23.28 25.814999999999998 27.595 50-54 23.225 23.74 24.805 28.23 55-59 22.745 23.44 25.165 28.65 60-64 23.28 22.875 26.290000000000003 27.555000000000003 65-69 23.25 23.565 25.009999999999998 28.175 70-74 22.939999999999998 24.474999999999998 25.324999999999996 27.26 75-79 22.725 24.25 24.305 28.720000000000002 80-84 23.665 24.13 24.775 27.43 85-89 23.34 24.675 23.565 28.42 90-94 23.935000000000002 23.215 24.965 27.884999999999998 95-99 23.599999999999998 24.035 24.345 28.02 100-104 23.855 24.635 24.05 27.46 105-109 23.61 23.44 24.365000000000002 28.585 110-114 24.235 24.39 23.875 27.500000000000004 115-119 23.580000000000002 24.595 23.515 28.310000000000002 120-124 23.36 24.77 24.165 27.705000000000002 125-129 23.695 23.799999999999997 24.615000000000002 27.889999999999997 130-134 23.625 24.695 24.060000000000002 27.62 135-139 23.525 25.1 23.974999999999998 27.400000000000002 140-144 24.490000000000002 24.51 23.405 27.595 145-149 23.78402722177742 24.239391513210567 24.649719775820657 27.32686148919135 150-151 23.974999999999998 23.65 24.675 27.700000000000003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 0.5 23 0.5 24 1.0 25 2.0 26 2.0 27 1.5 28 2.5 29 4.5 30 9.5 31 13.0 32 12.0 33 19.0 34 29.5 35 37.5 36 53.0 37 57.5 38 57.0 39 70.5 40 86.0 41 104.0 42 108.5 43 114.0 44 136.0 45 139.0 46 131.0 47 131.5 48 127.0 49 127.0 50 144.5 51 151.0 52 188.0 53 231.0 54 213.5 55 190.0 56 152.5 57 132.5 58 141.5 59 124.5 60 102.5 61 92.5 62 76.0 63 62.5 64 51.0 65 44.5 66 48.5 67 49.5 68 44.5 69 37.0 70 34.0 71 22.0 72 15.5 73 18.0 74 19.0 75 14.5 76 8.0 77 4.5 78 2.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.08 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.375 #Duplication Level Percentage of deduplicated Percentage of total 1 92.96671490593343 80.30000000000001 2 4.341534008683068 7.5 3 1.0709117221418234 2.775 4 0.40520984081041966 1.4000000000000001 5 0.31837916063675836 1.375 6 0.37626628075253254 1.95 7 0.11577424023154848 0.7000000000000001 8 0.05788712011577424 0.4 9 0.08683068017366136 0.675 >10 0.26049204052098407 2.9250000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTTGATCTCGTATGC 30 0.75 TruSeq Adapter, Index 1 (97% over 36bp) CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 13 0.325 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 12 0.3 No Hit GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 11 0.27499999999999997 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 11 0.27499999999999997 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 10 0.25 No Hit GTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATC 10 0.25 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 10 0.25 No Hit CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 10 0.25 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 9 0.22499999999999998 No Hit TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG 9 0.22499999999999998 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 9 0.22499999999999998 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 8 0.2 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 8 0.2 No Hit GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA 7 0.17500000000000002 No Hit GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG 7 0.17500000000000002 No Hit GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC 7 0.17500000000000002 No Hit CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCA 7 0.17500000000000002 No Hit CCGGAGTTTATATTACATGCCCCTCTCCCAACGATAGTTGTAGTACTCTT 6 0.15 No Hit CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA 6 0.15 No Hit GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC 6 0.15 No Hit GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT 6 0.15 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 6 0.15 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 6 0.15 No Hit CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 6 0.15 No Hit CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA 6 0.15 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 6 0.15 No Hit CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG 6 0.15 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 6 0.15 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 6 0.15 No Hit CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT 6 0.15 No Hit GTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCC 5 0.125 No Hit CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT 5 0.125 No Hit CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG 5 0.125 No Hit ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA 5 0.125 No Hit GATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCT 5 0.125 No Hit GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC 5 0.125 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 5 0.125 No Hit GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG 5 0.125 No Hit CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT 5 0.125 No Hit GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC 5 0.125 No Hit AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.2625 0.0 0.0 0.0 0.0 78-79 0.2875 0.0 0.0 0.0 0.0 80-81 0.32499999999999996 0.0 0.0 0.0 0.0 82-83 0.4125 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.6625 0.0 0.0 0.0 0.0 88-89 0.825 0.0 0.0 0.0 0.0 90-91 1.0 0.0 0.0 0.0 0.0 92-93 1.2 0.0 0.0 0.0 0.0 94-95 1.3875000000000002 0.0 0.0 0.0 0.0 96-97 1.7375 0.0 0.0 0.0 0.0 98-99 2.0250000000000004 0.0 0.0 0.0 0.0 100-101 2.4 0.0 0.0 0.0 0.0 102-103 2.7125 0.0 0.0 0.0 0.0 104-105 3.0625 0.0 0.0 0.0 0.0 106-107 3.55 0.0 0.0 0.0 0.0 108-109 4.05 0.0 0.0 0.0 0.0 110-111 4.55 0.0 0.0 0.0 0.0 112-113 5.1125 0.0 0.0 0.0 0.0 114-115 5.65 0.0 0.0 0.0 0.0 116-117 6.4625 0.0 0.0 0.0 0.0 118-119 6.9 0.0 0.0 0.0 0.0 120-121 7.4375 0.0 0.0 0.0 0.0 122-123 7.9125000000000005 0.0 0.0 0.0 0.0 124-125 8.6125 0.0 0.0 0.0 0.0 126-127 9.275 0.0 0.0 0.0 0.0 128-129 10.0625 0.0 0.0 0.0 0.0 130-131 10.75 0.0 0.0 0.0 0.0 132-133 11.3625 0.0 0.0 0.0 0.0 134-135 11.9625 0.0 0.0 0.0 0.0 136-137 12.725 0.0 0.0 0.0 0.0 138-139 13.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCT 10 0.006830828 145.0 8 ATTAACG 10 0.006830828 145.0 6 GATTAAC 10 0.006830828 145.0 5 ATGTACA 10 0.006830828 145.0 6 AATGTAC 10 0.006830828 145.0 5 CCGATTA 10 0.006830828 145.0 3 ACTTTAT 20 0.00593511 29.0 95-99 TCCAATC 20 0.00593511 29.0 75-79 AAAAAAA 280 3.2634474E-5 7.25 65-69 >>END_MODULE SRR7473350 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473350_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.19275 34.0 33.0 34.0 33.0 34.0 2 33.22925 34.0 33.0 34.0 33.0 34.0 3 33.122 34.0 33.0 34.0 33.0 34.0 4 33.1085 34.0 33.0 34.0 33.0 34.0 5 33.20725 34.0 33.0 34.0 33.0 34.0 6 37.3005 38.0 38.0 38.0 37.0 38.0 7 37.153 38.0 38.0 38.0 37.0 38.0 8 37.29625 38.0 38.0 38.0 37.0 38.0 9 37.317 38.0 38.0 38.0 38.0 38.0 10-14 37.255449999999996 38.0 38.0 38.0 37.6 38.0 15-19 37.33135 38.0 38.0 38.0 38.0 38.0 20-24 37.3877 38.0 38.0 38.0 38.0 38.0 25-29 37.370400000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.4197 38.0 38.0 38.0 38.0 38.0 35-39 37.21305 38.0 38.0 38.0 37.2 38.0 40-44 37.269099999999995 38.0 38.0 38.0 37.6 38.0 45-49 37.29315 38.0 38.0 38.0 37.8 38.0 50-54 37.30565 38.0 38.0 38.0 37.8 38.0 55-59 37.3099 38.0 38.0 38.0 38.0 38.0 60-64 37.2678 38.0 38.0 38.0 37.4 38.0 65-69 37.0216 38.0 38.0 38.0 37.0 38.0 70-74 36.977199999999996 38.0 38.0 38.0 37.0 38.0 75-79 36.90259999999999 38.0 38.0 38.0 36.8 38.0 80-84 36.8161 38.0 38.0 38.0 36.0 38.0 85-89 36.7469 38.0 38.0 38.0 36.0 38.0 90-94 36.615449999999996 38.0 38.0 38.0 35.6 38.0 95-99 36.57915 38.0 38.0 38.0 35.4 38.0 100-104 36.50695 38.0 38.0 38.0 35.0 38.0 105-109 36.2889 38.0 38.0 38.0 34.4 38.0 110-114 35.9773 38.0 38.0 38.0 33.8 38.0 115-119 35.5713 38.0 37.4 38.0 31.6 38.0 120-124 35.8192 38.0 38.0 38.0 33.2 38.0 125-129 35.700300000000006 38.0 37.6 38.0 32.6 38.0 130-134 35.3387 38.0 36.0 38.0 31.2 38.0 135-139 35.0301 38.0 35.8 38.0 30.0 38.0 140-144 34.29365 38.0 34.4 38.0 26.0 38.0 145-149 33.6611 38.0 33.2 38.0 22.2 38.0 150-151 29.163874999999997 35.5 24.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 1.0 5 1.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 3.0 12 1.0 13 2.0 14 6.0 15 5.0 16 6.0 17 26.0 18 4.0 19 3.0 20 7.0 21 9.0 22 5.0 23 7.0 24 6.0 25 5.0 26 5.0 27 16.0 28 21.0 29 31.0 30 19.0 31 38.0 32 65.0 33 85.0 34 132.0 35 178.0 36 537.0 37 2769.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.125 15.55 12.775 33.550000000000004 2 31.506506506506504 21.896896896896898 24.84984984984985 21.746746746746748 3 24.67434869739479 25.250501002004004 24.9749498997996 25.100200400801604 4 27.432296890672013 32.422266800401204 18.029087261785357 22.116349047141423 5 32.014028056112224 31.2875751503006 16.30761523046092 20.390781563126254 6 25.900000000000002 35.375 17.724999999999998 21.0 7 22.825 17.675 33.550000000000004 25.95 8 26.85 22.425 21.8 28.925 9 26.35 23.625 23.825 26.200000000000003 10-14 28.13703425856464 24.566141535383846 21.410352588147035 25.886471617904476 15-19 28.555711142228446 24.48989797959592 22.089417883576715 24.86497299459892 20-24 28.942894289428946 24.56745674567457 21.907190719071906 24.582458245824583 25-29 27.794728154854198 25.698994648126845 21.892662431851146 24.61361476516781 30-34 28.689999999999998 25.790000000000003 21.81 23.71 35-39 27.934190128519276 25.12876931539731 22.348352252837923 24.588688303245487 40-44 28.972897289728973 24.097409740974097 22.967296729672967 23.962396239623963 45-49 28.49569913982797 24.97999599919984 22.634526905381076 23.889777955591118 50-54 28.175635127025405 24.60992198439688 22.984596919383876 24.22984596919384 55-59 28.055611122224445 24.95999199839968 23.504700940188037 23.479695939187835 60-64 27.452353559101596 25.39642839277675 22.71021959881947 24.440998449302185 65-69 28.54282424788507 25.274065174951193 23.141612854783 23.04149772238074 70-74 28.304245636845526 25.0087513126969 22.93844076611492 23.74856228434265 75-79 28.22641132056603 25.011250562528126 22.50612530626531 24.25621281064053 80-84 29.02580516103221 24.81996399279856 22.774554910982197 23.379675935187038 85-89 29.275000000000002 24.88 22.365 23.48 90-94 28.680038013304653 24.908718051317962 22.832991547041466 23.57825238833592 95-99 28.360000000000003 25.679999999999996 22.900000000000002 23.06 100-104 28.799999999999997 25.165 22.545 23.49 105-109 28.118435530659198 26.297889366810047 22.321696508952684 23.26197859357807 110-114 28.94723655860099 25.379566067044145 23.039535000250538 22.633662374104325 115-119 29.34155759490497 26.588435885863298 21.829396720324958 22.240609798906775 120-124 28.851638729046787 25.208906680010006 23.137353014761068 22.80210157618214 125-129 29.125 25.724999999999998 22.75 22.400000000000002 130-134 28.694999999999997 25.735000000000003 22.97 22.6 135-139 29.26 25.740000000000002 22.555 22.445 140-144 30.130000000000003 25.445 22.07 22.355 145-149 29.7 26.69 22.045 21.565 150-151 29.45 26.9125 22.0125 21.625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.5 22 1.0 23 3.5 24 3.5 25 0.5 26 2.0 27 3.5 28 6.0 29 9.0 30 8.0 31 8.5 32 10.0 33 14.5 34 18.5 35 26.0 36 35.0 37 39.5 38 42.0 39 50.0 40 64.5 41 70.5 42 86.5 43 102.5 44 113.0 45 112.5 46 122.0 47 131.5 48 140.5 49 158.0 50 159.0 51 157.0 52 171.5 53 222.0 54 240.0 55 213.0 56 163.5 57 120.0 58 114.0 59 110.0 60 98.0 61 105.0 62 113.0 63 86.0 64 66.5 65 59.5 66 52.0 67 55.5 68 56.0 69 52.0 70 47.0 71 42.0 72 33.0 73 20.0 74 16.0 75 16.0 76 12.0 77 7.0 78 2.5 79 2.0 80 2.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.2 4 0.3 5 0.2 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.025 15-19 0.02 20-24 0.01 25-29 0.034999999999999996 30-34 0.0 35-39 0.015 40-44 0.01 45-49 0.02 50-54 0.02 55-59 0.02 60-64 0.045 65-69 0.11499999999999999 70-74 0.015 75-79 0.005 80-84 0.02 85-89 0.0 90-94 0.034999999999999996 95-99 0.0 100-104 0.0 105-109 0.03 110-114 0.215 115-119 0.295 120-124 0.075 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.7 #Duplication Level Percentage of deduplicated Percentage of total 1 93.70011402508553 82.175 2 4.161915621436716 7.3 3 0.9692132269099202 2.55 4 0.31356898517673887 1.0999999999999999 5 0.17103762827822122 0.75 6 0.19954389965792474 1.05 7 0.14253135689851767 0.8750000000000001 8 0.08551881413911061 0.6 9 0.05701254275940707 0.44999999999999996 >10 0.19954389965792474 3.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 30 0.75 Illumina Single End PCR Primer 1 (100% over 50bp) CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 28 0.7000000000000001 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 16 0.4 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 16 0.4 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 15 0.375 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 11 0.27499999999999997 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 10 0.25 No Hit GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG 9 0.22499999999999998 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 9 0.22499999999999998 No Hit CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT 8 0.2 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 8 0.2 No Hit GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG 8 0.2 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 7 0.17500000000000002 No Hit GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGAT 7 0.17500000000000002 No Hit CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT 7 0.17500000000000002 No Hit CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT 7 0.17500000000000002 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 7 0.17500000000000002 No Hit GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA 6 0.15 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 6 0.15 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 6 0.15 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 6 0.15 No Hit AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG 6 0.15 No Hit CGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGG 6 0.15 No Hit CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG 6 0.15 No Hit GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 5 0.125 No Hit GTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATC 5 0.125 No Hit GCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTGAGGCGTGATGACGA 5 0.125 No Hit ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG 5 0.125 No Hit GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGC 5 0.125 No Hit ACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.2625 0.0 0.0 0.0 0.0 78-79 0.2875 0.0 0.0 0.0 0.0 80-81 0.32499999999999996 0.0 0.0 0.0 0.0 82-83 0.4125 0.0 0.0 0.0 0.0 84-85 0.5 0.0 0.0 0.0 0.0 86-87 0.6375 0.0 0.0 0.0 0.0 88-89 0.8 0.0 0.0 0.0 0.0 90-91 0.975 0.0 0.0 0.0 0.0 92-93 1.2125 0.0 0.0 0.0 0.0 94-95 1.4125 0.0 0.0 0.0 0.0 96-97 1.7875 0.0 0.0 0.0 0.0 98-99 2.075 0.0 0.0 0.0 0.0 100-101 2.425 0.0 0.0 0.0 0.0 102-103 2.7125 0.0 0.0 0.0 0.0 104-105 3.0375 0.0 0.0 0.0 0.0 106-107 3.525 0.0 0.0 0.0 0.0 108-109 4.025 0.0 0.0 0.0 0.0 110-111 4.5125 0.0 0.0 0.0 0.0 112-113 5.074999999999999 0.0 0.0 0.0 0.0 114-115 5.5625 0.0 0.0 0.0 0.0 116-117 6.362500000000001 0.0 0.0 0.0 0.0 118-119 6.800000000000001 0.0 0.0 0.0 0.0 120-121 7.3125 0.0 0.0 0.0 0.0 122-123 7.7625 0.0 0.0 0.0 0.0 124-125 8.4375 0.0 0.0 0.0 0.0 126-127 9.125 0.0 0.0 0.0 0.0 128-129 9.875 0.0 0.0 0.0 0.0 130-131 10.575 0.0 0.0 0.0 0.0 132-133 11.1875 0.0 0.0 0.0 0.0 134-135 11.8125 0.0 0.0 0.0 0.0 136-137 12.6125 0.0 0.0 0.0 0.0 138-139 13.399999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGCAG 10 0.006843168 144.91249 145 AAAAAAA 155 2.8928934E-4 9.349194 60-64 >>END_MODULE Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812296 spots for SRR7473350.sra Written 812296 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra Read 812290 spots for SRR7473350.sra Written 812290 spots for SRR7473350.sra SRR ids: ['SRR7473350.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_p6qtkoww SRR7473350.sra spots: 16245806 blocks: [[1, 812290], [812291, 1624580], [1624581, 2436870], [2436871, 3249160], [3249161, 4061450], [4061451, 4873740], [4873741, 5686030], [5686031, 6498320], [6498321, 7310610], [7310611, 8122900], [8122901, 8935190], [8935191, 9747480], [9747481, 10559770], [10559771, 11372060], [11372061, 12184350], [12184351, 12996640], [12996641, 13808930], [13808931, 14621220], [14621221, 15433510], [15433511, 16245806]] SRR7473350 file size 5483470 SRR7473350 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473350 SRR7473350_1.fastq SRR7473350_2.fastq Input file: SRR7473350_1.fastq Paired file: SRR7473350_2.fastq trimmed: SRR7473350-trimmed-pair1.fastq, SRR7473350-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 14:31:09 2024 >> started Sat Dec 7 14:31:27 2024 >> done (18.484s) 16245806 read pairs processed; of these: 20156 ( 0.12%) short read pairs filtered out after trimming by size control 133334 ( 0.82%) empty read pairs filtered out after trimming by size control 16092316 (99.06%) read pairs available; of these: 7968038 (49.51%) trimmed read pairs available after processing 8124278 (50.49%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 19 0.00% 20 7 0.00% 21 13 0.00% 22 11 0.00% 23 13 0.00% 24 17 0.00% 25 15 0.00% 26 16 0.00% 27 26 0.00% 28 30 0.00% 29 50 0.00% 30 44 0.00% 31 46 0.00% 32 57 0.00% 33 60 0.00% 34 43 0.00% 35 71 0.00% 36 51 0.00% 37 51 0.00% 38 71 0.00% 39 58 0.00% 40 92 0.00% 41 94 0.00% 42 117 0.00% 43 89 0.00% 44 138 0.00% 45 146 0.00% 46 190 0.00% 47 175 0.00% 48 206 0.00% 49 253 0.00% 50 287 0.00% 51 296 0.00% 52 380 0.00% 53 385 0.00% 54 331 0.00% 55 393 0.00% 56 419 0.00% 57 502 0.00% 58 543 0.00% 59 607 0.00% 60 724 0.00% 61 842 0.01% 62 989 0.01% 63 1022 0.01% 64 1223 0.01% 65 1506 0.01% 66 1492 0.01% 67 1721 0.01% 68 2823 0.02% 69 11060 0.07% 70 14580 0.09% 71 5927 0.04% 72 4055 0.03% 73 4140 0.03% 74 4046 0.03% 75 4035 0.03% 76 4187 0.03% 77 4490 0.03% 78 5134 0.03% 79 5764 0.04% 80 6294 0.04% 81 7115 0.04% 82 8162 0.05% 83 9498 0.06% 84 12330 0.08% 85 13228 0.08% 86 13894 0.09% 87 14510 0.09% 88 16861 0.10% 89 17171 0.11% 90 18447 0.11% 91 19447 0.12% 92 19396 0.12% 93 24319 0.15% 94 24540 0.15% 95 28861 0.18% 96 28025 0.17% 97 29479 0.18% 98 29000 0.18% 99 29185 0.18% 100 32489 0.20% 101 30914 0.19% 102 33264 0.21% 103 33706 0.21% 104 36534 0.23% 105 41466 0.26% 106 39220 0.24% 107 38864 0.24% 108 41478 0.26% 109 48195 0.30% 110 50424 0.31% 111 44432 0.28% 112 45219 0.28% 113 54684 0.34% 114 49354 0.31% 115 54646 0.34% 116 56864 0.35% 117 53531 0.33% 118 54346 0.34% 119 54314 0.34% 120 57334 0.36% 121 54294 0.34% 122 59285 0.37% 123 63653 0.40% 124 63163 0.39% 125 63363 0.39% 126 63904 0.40% 127 66540 0.41% 128 66304 0.41% 129 66803 0.42% 130 69816 0.43% 131 69701 0.43% 132 71409 0.44% 133 75002 0.47% 134 80577 0.50% 135 82421 0.51% 136 82694 0.51% 137 90857 0.56% 138 91615 0.57% 139 93885 0.58% 140 94155 0.59% 141 102608 0.64% 142 104812 0.65% 143 112339 0.70% 144 123716 0.77% 145 137790 0.86% 146 159847 0.99% 147 200070 1.24% 148 287662 1.79% 149 558540 3.47% 150 3303987 20.53% 151 8124278 50.49% 16092316 reads passed initial QC criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=2.54 fanout-score-rank=25 prefix-density=0.69 prefix-fanout=2.4 sequence=GGTGTTGTCGAAGCCGATGATGCGGACATAGGCGTCAGGGTACTCCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=17.26 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=1.8 sequence=GCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACCGGGCAGTGCCATTGGCATGACAACCCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCTACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACTCTTGGGCGGTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATTCAGAACCACCGGATCACTAT criterion=sequence-density sequence-density=2.79 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=33 prefix-density=2.82 prefix-fanout=2.0 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.14 sequence-density-rank=28 fanout-score=23.19 fanout-score-rank=1 prefix-density=2.62 prefix-fanout=1.2 sequence=GGCGAGGCCGTCTGGTTCAAGGCCGGCTCCCAGATCTTCAGCGAGGGCGGCCTCGACTACCTTGGCAACCCGAGCCTCGTCCATGCCCAGAGCAT SRR7473350 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 14:32:47 Started mapping on | Dec 07 14:32:47 Finished on | Dec 07 14:48:32 Mapping speed, Million of reads per hour | 61.30 Number of input reads | 16092316 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 10863839 Uniquely mapped reads % | 67.51% Average mapped length | 292.15 Number of splices: Total | 10002397 Number of splices: Annotated (sjdb) | 9433320 Number of splices: GT/AG | 9874734 Number of splices: GC/AG | 112025 Number of splices: AT/AC | 4028 Number of splices: Non-canonical | 11610 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.01% Deletion average length | 1.44 Insertion rate per base | 0.01% Insertion average length | 1.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 156828 % of reads mapped to multiple loci | 0.97% Number of reads mapped to too many loci | 19111 % of reads mapped to too many loci | 0.12% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 29.10% % of reads unmapped: other | 2.29% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5080186 5080186 5080186 N_multimapping 156828 156828 156828 N_noFeature 376377 10504547 490735 N_ambiguous 285439 1517 40645 UnstrandedReadsAssigned:10202023 PositiveStrandReadsAssigned:357775 NegativeStrandReadsAssigned:10332459 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=146 echo kmer=141 SRR7473350 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473350-trimmed-pair1.fastq SRR7473350-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,092,316 reads, 10,406,560 reads pseudoaligned [quant] estimated average fragment length: 240.127 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,043 rounds 52973 SRR7473350.ke.tsv 35125 SRR7473350.se.tsv 88098 total ==> SRR7473350.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 697.185 11.8813 1.86482 PNS24247 1044 804.873 14.9482 2.03228 PNS24249 1928 1688.87 29.4813 1.91016 PNS24246 1044 804.873 14.9482 2.03228 PNS24248 1044 804.873 14.9482 2.03228 PNS24244 1471 1231.87 63.7928 5.66666 PNS24243 293 101.618 0 0 KQK14069 1603 1363.87 441.721 35.4401 KQK14071 474 249.548 10.3609 4.54323 ==> SRR7473350.se.tsv <== BRADI_1g14170v3 491 BRADI_1g53295v3 21 BRADI_1g59795v3 470 BRADI_1g07683v3 0 BRADI_1g00485v3 16 BRADI_1g20270v3 1089 BRADI_1g74790v3 102 BRADI_1g09890v3 7 BRADI_1g77505v3 242 BRADI_1g48960v3 0 SRR7473350 completed mapping pipeline successfully