Starting /dee2/code/volunteer_pipeline.sh SRR7473351 current disk space = 1542972571648 free memory = 1603316456 SRR7473351 SRAfilesize 40e374ddf878f0e75a892819d132aa72 SRR7473351.sra SRR7473351.sra file validated SRR7473351 is paired end SRR7473351 is conventional basespace SRR7473351 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473351_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.571 34.0 34.0 34.0 33.0 34.0 2 33.61725 34.0 34.0 34.0 33.0 34.0 3 33.65 34.0 34.0 34.0 33.0 34.0 4 33.57375 34.0 34.0 34.0 33.0 34.0 5 33.6215 34.0 34.0 34.0 33.0 34.0 6 37.331 38.0 38.0 38.0 37.0 38.0 7 37.5735 38.0 38.0 38.0 38.0 38.0 8 37.6405 38.0 38.0 38.0 38.0 38.0 9 37.632 38.0 38.0 38.0 38.0 38.0 10-14 37.665350000000004 38.0 38.0 38.0 38.0 38.0 15-19 37.65665 38.0 38.0 38.0 38.0 38.0 20-24 37.70605 38.0 38.0 38.0 38.0 38.0 25-29 37.629000000000005 38.0 38.0 38.0 38.0 38.0 30-34 37.5646 38.0 38.0 38.0 37.8 38.0 35-39 37.4949 38.0 38.0 38.0 37.8 38.0 40-44 37.45934999999999 38.0 38.0 38.0 38.0 38.0 45-49 37.414 38.0 38.0 38.0 37.6 38.0 50-54 37.41775 38.0 38.0 38.0 37.4 38.0 55-59 37.18465 38.0 38.0 38.0 36.8 38.0 60-64 37.2392 38.0 38.0 38.0 37.0 38.0 65-69 36.98864999999999 38.0 38.0 38.0 35.6 38.0 70-74 37.2649 38.0 38.0 38.0 37.0 38.0 75-79 37.03385 38.0 38.0 38.0 37.0 38.0 80-84 37.00515 38.0 38.0 38.0 36.8 38.0 85-89 36.871249999999996 38.0 38.0 38.0 36.2 38.0 90-94 36.717600000000004 38.0 38.0 38.0 35.6 38.0 95-99 36.743449999999996 38.0 38.0 38.0 35.8 38.0 100-104 36.59555 38.0 38.0 38.0 35.2 38.0 105-109 36.521100000000004 38.0 38.0 38.0 34.8 38.0 110-114 36.4012 38.0 38.0 38.0 34.4 38.0 115-119 36.3465 38.0 38.0 38.0 34.2 38.0 120-124 36.096000000000004 38.0 38.0 38.0 33.8 38.0 125-129 35.64549999999999 38.0 37.0 38.0 32.2 38.0 130-134 35.43665 38.0 36.4 38.0 31.2 38.0 135-139 34.8367 38.0 35.4 38.0 27.8 38.0 140-144 34.754149999999996 38.0 35.6 38.0 29.4 38.0 145-149 34.26989999999999 38.0 34.6 38.0 27.2 38.0 150-151 29.617375000000003 35.5 27.0 38.0 11.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 1.0 13 2.0 14 4.0 15 5.0 16 3.0 17 3.0 18 11.0 19 15.0 20 4.0 21 1.0 22 4.0 23 7.0 24 9.0 25 11.0 26 9.0 27 16.0 28 19.0 29 24.0 30 31.0 31 45.0 32 44.0 33 65.0 34 97.0 35 161.0 36 531.0 37 2877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.1 12.25 11.275 37.375 2 25.35633908477119 13.653413353338333 34.15853963490873 26.831707926981746 3 22.325 18.6 29.425 29.65 4 23.400000000000002 27.400000000000002 24.075 25.124999999999996 5 26.400000000000002 28.1 25.424999999999997 20.075000000000003 6 19.8 29.5 28.475 22.225 7 15.65 20.349999999999998 44.3 19.7 8 18.825 22.2 29.975 28.999999999999996 9 19.175 20.45 32.7 27.675 10-14 21.884999999999998 24.41 25.635 28.07 15-19 22.28 24.855 27.41 25.455 20-24 22.075 24.55 27.169999999999998 26.205000000000002 25-29 20.575 24.955 28.634999999999998 25.835 30-34 20.32 25.130000000000003 27.305 27.245 35-39 21.395 25.035 27.744999999999997 25.825 40-44 22.295 24.595 26.915 26.195 45-49 23.025000000000002 24.275 27.365000000000002 25.335 50-54 22.56 25.365 26.55 25.525 55-59 20.835 25.025 28.16 25.979999999999997 60-64 20.974999999999998 24.625 28.544999999999998 25.855 65-69 21.19 25.009999999999998 27.47 26.33 70-74 21.4 25.82 26.650000000000002 26.13 75-79 21.044999999999998 26.75 26.035000000000004 26.169999999999998 80-84 21.18 26.165 26.72 25.935000000000002 85-89 21.255 25.64 26.11 26.995 90-94 21.84 24.75 26.665 26.745 95-99 22.040000000000003 25.385 26.99 25.585 100-104 22.175 25.7 25.89 26.235000000000003 105-109 21.45 25.369999999999997 26.525 26.655 110-114 20.905 25.605 26.11 27.38 115-119 21.525 26.284999999999997 26.064999999999998 26.125 120-124 21.61 26.400000000000002 25.355 26.634999999999998 125-129 22.575 25.385 27.105 24.935 130-134 21.55155155155155 27.107107107107108 26.09109109109109 25.25025025025025 135-139 20.858343337334933 26.57062825130052 26.830732292917165 25.740296118447382 140-144 22.634999999999998 26.450000000000003 24.93 25.985000000000003 145-149 22.785 26.195 25.080000000000002 25.94 150-151 21.575 25.95 25.587500000000002 26.887499999999996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 1.5 20 1.5 21 0.5 22 0.0 23 1.5 24 1.5 25 0.0 26 1.5 27 2.5 28 3.0 29 13.0 30 23.0 31 28.5 32 34.5 33 55.0 34 69.5 35 74.0 36 105.0 37 124.5 38 131.5 39 120.0 40 89.5 41 86.0 42 83.0 43 77.5 44 98.5 45 103.5 46 108.5 47 122.0 48 125.0 49 148.0 50 172.0 51 175.5 52 192.0 53 280.5 54 320.0 55 258.0 56 180.0 57 136.0 58 129.0 59 105.5 60 66.0 61 48.5 62 45.0 63 24.5 64 9.0 65 4.5 66 3.0 67 3.0 68 3.0 69 2.0 70 1.0 71 0.5 72 1.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.1 135-139 0.04 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.875 #Duplication Level Percentage of deduplicated Percentage of total 1 78.292220113852 51.575 2 11.157495256166982 14.7 3 4.554079696394687 9.0 4 2.239089184060721 5.8999999999999995 5 1.1005692599620494 3.6249999999999996 6 0.9867172675521821 3.9 7 0.5313092979127134 2.45 8 0.22770398481973433 1.2 9 0.22770398481973433 1.35 >10 0.683111954459203 6.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 31 0.775 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC 22 0.5499999999999999 TruSeq Adapter, Index 2 (97% over 37bp) CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 19 0.475 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 15 0.375 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 15 0.375 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 15 0.375 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 14 0.35000000000000003 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 14 0.35000000000000003 No Hit AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA 13 0.325 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 12 0.3 No Hit GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC 11 0.27499999999999997 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCC 11 0.27499999999999997 TruSeq Adapter, Index 2 (97% over 36bp) GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC 10 0.25 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 10 0.25 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 10 0.25 No Hit CGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCT 10 0.25 No Hit GTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATC 10 0.25 No Hit GTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCAC 10 0.25 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 9 0.22499999999999998 No Hit CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA 9 0.22499999999999998 No Hit CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC 9 0.22499999999999998 No Hit GCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGA 9 0.22499999999999998 No Hit GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG 9 0.22499999999999998 No Hit CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCT 9 0.22499999999999998 No Hit CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA 8 0.2 No Hit GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC 8 0.2 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 8 0.2 No Hit GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT 8 0.2 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 8 0.2 No Hit GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT 8 0.2 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 7 0.17500000000000002 No Hit GGGAGAACCAGCTATCTCCCGGTTTGATTGGCCTTTCACCCCCAGCCACA 7 0.17500000000000002 No Hit GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC 7 0.17500000000000002 No Hit CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC 7 0.17500000000000002 No Hit CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT 7 0.17500000000000002 No Hit GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTA 7 0.17500000000000002 No Hit GCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCT 7 0.17500000000000002 No Hit CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT 7 0.17500000000000002 No Hit GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC 7 0.17500000000000002 No Hit GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG 7 0.17500000000000002 No Hit CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG 7 0.17500000000000002 No Hit CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT 7 0.17500000000000002 No Hit CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC 7 0.17500000000000002 No Hit CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC 7 0.17500000000000002 No Hit GCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAA 6 0.15 No Hit ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG 6 0.15 No Hit GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTA 6 0.15 No Hit CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAG 6 0.15 No Hit GGCAAAAACAGGCAAGCCGGTGATTTTATCTACAGGAATGTCTGATATTG 6 0.15 No Hit GTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGA 6 0.15 No Hit GGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTAC 6 0.15 No Hit GCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGC 6 0.15 No Hit GGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGAC 6 0.15 No Hit CCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTAC 6 0.15 No Hit CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT 6 0.15 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 6 0.15 No Hit GGCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAACATTAGTC 6 0.15 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 6 0.15 No Hit GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC 6 0.15 No Hit CTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 6 0.15 No Hit GGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATGGATGCAGGGAT 6 0.15 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 6 0.15 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 6 0.15 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 6 0.15 No Hit CGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCCC 6 0.15 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 6 0.15 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 6 0.15 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 6 0.15 No Hit GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA 6 0.15 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 6 0.15 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 5 0.125 No Hit CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG 5 0.125 No Hit GTTTGATTGGCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAA 5 0.125 No Hit GCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGTG 5 0.125 No Hit GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG 5 0.125 No Hit GTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGG 5 0.125 No Hit CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT 5 0.125 No Hit CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC 5 0.125 No Hit GGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTT 5 0.125 No Hit ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA 5 0.125 No Hit GCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGG 5 0.125 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 5 0.125 No Hit CAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAA 5 0.125 No Hit CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG 5 0.125 No Hit CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT 5 0.125 No Hit CAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTAC 5 0.125 No Hit GGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGG 5 0.125 No Hit GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGA 5 0.125 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 5 0.125 No Hit TGGGAATACTCGCCCCAGTAGTTTCTGTTGCCTTAGGAGCGGATGTTATT 5 0.125 No Hit ATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG 5 0.125 No Hit GCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACAC 5 0.125 No Hit CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTA 5 0.125 No Hit GCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAG 5 0.125 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 5 0.125 No Hit ATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGC 5 0.125 No Hit CTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTG 5 0.125 No Hit CGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTT 5 0.125 No Hit GCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2125 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.7250000000000001 0.0 0.0 0.0 0.0 90-91 0.875 0.0 0.0 0.0 0.0 92-93 1.125 0.0 0.0 0.0 0.0 94-95 1.425 0.0 0.0 0.0 0.0 96-97 1.6875 0.0 0.0 0.0 0.0 98-99 1.9625 0.0 0.0 0.0 0.0 100-101 2.2750000000000004 0.0 0.0 0.0 0.0 102-103 2.5999999999999996 0.0 0.0 0.0 0.0 104-105 2.8875 0.0 0.0 0.0 0.0 106-107 3.2875 0.0 0.0 0.0 0.0 108-109 3.625 0.0 0.0 0.0 0.0 110-111 3.9375 0.0 0.0 0.0 0.0 112-113 4.35 0.0 0.0 0.0 0.0 114-115 4.975 0.0 0.0 0.0 0.0 116-117 5.6625 0.0 0.0 0.0 0.0 118-119 6.225 0.0 0.0 0.0 0.0 120-121 6.85 0.0 0.0 0.0 0.0 122-123 7.5875 0.0 0.0 0.0 0.0 124-125 8.175 0.0 0.0 0.0 0.0 126-127 8.7 0.0 0.0 0.0 0.0 128-129 9.1375 0.0 0.0 0.0 0.0 130-131 9.774999999999999 0.0 0.0 0.0 0.0 132-133 10.4375 0.0 0.0 0.0 0.0 134-135 11.175 0.0 0.0 0.0 0.0 136-137 12.25 0.0 0.0 0.0 0.0 138-139 13.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGTG 10 0.006843168 144.91249 2 AGGATGA 10 0.006843168 144.91249 4 GATGAGT 10 0.006843168 144.91249 6 GAGTCCC 10 0.006843168 144.91249 9 GTCTCCC 10 0.006843168 144.91249 8 GCCGTGT 10 0.006843168 144.91249 1 TGAGTCC 10 0.006843168 144.91249 8 TGTGTCT 10 0.006843168 144.91249 5 ATGAGTC 10 0.006843168 144.91249 7 >>END_MODULE SRR7473351 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473351_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.13925 34.0 33.0 34.0 33.0 34.0 2 33.14575 34.0 33.0 34.0 33.0 34.0 3 33.1025 34.0 33.0 34.0 33.0 34.0 4 32.97725 34.0 33.0 34.0 33.0 34.0 5 33.0485 34.0 33.0 34.0 33.0 34.0 6 36.65425 38.0 38.0 38.0 36.0 38.0 7 36.9345 38.0 38.0 38.0 37.0 38.0 8 36.96075 38.0 38.0 38.0 37.0 38.0 9 37.05875 38.0 38.0 38.0 37.0 38.0 10-14 37.06805 38.0 38.0 38.0 37.4 38.0 15-19 37.0333 38.0 38.0 38.0 37.4 38.0 20-24 37.035399999999996 38.0 38.0 38.0 37.4 38.0 25-29 37.1228 38.0 38.0 38.0 38.0 38.0 30-34 37.165299999999995 38.0 38.0 38.0 38.0 38.0 35-39 37.10105 38.0 38.0 38.0 38.0 38.0 40-44 37.089800000000004 38.0 38.0 38.0 38.0 38.0 45-49 36.99085 38.0 38.0 38.0 37.2 38.0 50-54 37.083650000000006 38.0 38.0 38.0 37.6 38.0 55-59 37.11855 38.0 38.0 38.0 38.0 38.0 60-64 37.0747 38.0 38.0 38.0 37.4 38.0 65-69 36.94955 38.0 38.0 38.0 37.0 38.0 70-74 36.7372 38.0 38.0 38.0 37.0 38.0 75-79 36.7161 38.0 38.0 38.0 37.0 38.0 80-84 36.6594 38.0 38.0 38.0 37.0 38.0 85-89 36.5055 38.0 38.0 38.0 36.0 38.0 90-94 36.460300000000004 38.0 38.0 38.0 35.6 38.0 95-99 36.40105 38.0 38.0 38.0 35.2 38.0 100-104 36.22165 38.0 38.0 38.0 34.8 38.0 105-109 36.05649999999999 38.0 38.0 38.0 34.4 38.0 110-114 35.573750000000004 38.0 38.0 38.0 32.4 38.0 115-119 35.64614999999999 38.0 38.0 38.0 33.2 38.0 120-124 35.0828 38.0 36.4 38.0 28.2 38.0 125-129 35.24114999999999 38.0 37.0 38.0 31.2 38.0 130-134 35.00755 38.0 36.2 38.0 31.0 38.0 135-139 34.76035 38.0 36.0 38.0 28.8 38.0 140-144 34.07025 38.0 35.6 38.0 25.2 38.0 145-149 33.01645 38.0 33.4 38.0 15.6 38.0 150-151 27.850625 34.0 17.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 5.0 4 3.0 5 1.0 6 1.0 7 2.0 8 4.0 9 1.0 10 7.0 11 6.0 12 4.0 13 3.0 14 2.0 15 4.0 16 4.0 17 25.0 18 5.0 19 6.0 20 4.0 21 8.0 22 7.0 23 7.0 24 9.0 25 9.0 26 14.0 27 30.0 28 16.0 29 25.0 30 21.0 31 39.0 32 49.0 33 75.0 34 106.0 35 179.0 36 518.0 37 2786.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.8 18.9 11.275 29.025000000000002 2 30.675 23.599999999999998 26.724999999999998 19.0 3 24.706176544136035 24.93123280820205 28.157039259814955 22.20555138784696 4 26.65666416604151 33.13328332083021 22.48062015503876 17.72943235808952 5 28.95723930982746 33.458364591147784 18.37959489872468 19.204801200300075 6 25.15 37.95 17.875 19.025 7 21.475 20.525 35.375 22.625 8 26.950000000000003 23.599999999999998 21.825 27.625 9 24.5 24.075 25.074999999999996 26.35 10-14 26.815 26.71 22.06 24.415 15-19 28.139999999999997 26.165 22.97 22.725 20-24 28.785 26.525 22.470000000000002 22.220000000000002 25-29 27.0 27.205000000000002 23.805 21.990000000000002 30-34 28.585 26.965 23.16 21.29 35-39 27.61 25.81 24.08 22.5 40-44 28.244999999999997 26.135 24.48 21.14 45-49 26.97 26.085 25.319999999999997 21.625 50-54 25.069999999999997 25.88 26.669999999999998 22.38 55-59 25.845000000000002 26.740000000000002 26.290000000000003 21.125 60-64 25.82 26.845000000000002 25.650000000000002 21.685 65-69 26.705000000000002 26.85 25.855 20.59 70-74 26.46 28.139999999999997 24.834999999999997 20.565 75-79 25.245 27.095000000000002 24.965 22.695 80-84 25.990000000000002 27.87 25.6 20.54 85-89 25.85 27.575 25.080000000000002 21.495 90-94 26.590000000000003 27.42 24.775 21.215 95-99 25.685000000000002 28.83 25.05 20.435 100-104 26.974999999999998 27.800000000000004 24.335 20.89 105-109 26.025 29.654999999999998 22.965 21.355 110-114 27.10771077107711 29.86798679867987 22.662266226622663 20.362036203620363 115-119 26.5489823473521 30.1045156773516 23.65854878231735 19.687953192978945 120-124 26.69 29.075 23.365 20.87 125-129 25.96 28.999999999999996 24.375 20.665 130-134 26.075 28.084999999999997 23.995 21.845 135-139 26.735 28.15 24.5 20.615 140-144 27.544999999999998 28.325 23.830000000000002 20.3 145-149 28.09 28.175 23.474999999999998 20.26 150-151 26.787499999999998 29.212500000000002 24.3625 19.6375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 0.5 23 1.0 24 0.5 25 0.5 26 0.5 27 2.5 28 5.0 29 8.5 30 15.0 31 25.0 32 30.5 33 33.0 34 67.5 35 78.0 36 80.0 37 118.0 38 131.5 39 119.5 40 126.0 41 102.0 42 57.5 43 53.0 44 66.0 45 68.0 46 69.5 47 105.5 48 152.5 49 168.0 50 174.5 51 164.5 52 198.0 53 299.5 54 330.0 55 295.5 56 235.5 57 169.0 58 120.0 59 90.5 60 62.0 61 48.0 62 51.0 63 36.0 64 13.5 65 5.0 66 5.5 67 2.5 68 2.5 69 2.0 70 1.0 71 2.0 72 1.0 73 0.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.025 4 0.025 5 0.025 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.01 115-119 0.015 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.25 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08812260536398 50.3 2 13.448275862068964 17.549999999999997 3 4.444444444444445 8.7 4 1.6091954022988506 4.2 5 1.1111111111111112 3.6249999999999996 6 0.45977011494252873 1.7999999999999998 7 0.2681992337164751 1.225 8 0.2681992337164751 1.4000000000000001 9 0.42145593869731796 2.475 >10 0.8812260536398467 8.725 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 36 0.8999999999999999 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 24 0.6 Illumina Single End PCR Primer 1 (100% over 50bp) GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 23 0.575 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 21 0.525 No Hit CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 20 0.5 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 20 0.5 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 17 0.42500000000000004 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 16 0.4 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 14 0.35000000000000003 No Hit GGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGA 13 0.325 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 12 0.3 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 12 0.3 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 12 0.3 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 12 0.3 No Hit GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 11 0.27499999999999997 No Hit GTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTT 11 0.27499999999999997 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 11 0.27499999999999997 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 11 0.27499999999999997 No Hit ATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCAT 11 0.27499999999999997 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 11 0.27499999999999997 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 11 0.27499999999999997 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 10 0.25 No Hit CGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTT 10 0.25 No Hit AATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCA 9 0.22499999999999998 No Hit GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA 9 0.22499999999999998 No Hit GGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATG 9 0.22499999999999998 No Hit GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG 9 0.22499999999999998 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 9 0.22499999999999998 No Hit CCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT 9 0.22499999999999998 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 9 0.22499999999999998 No Hit ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC 9 0.22499999999999998 No Hit CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT 9 0.22499999999999998 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 9 0.22499999999999998 No Hit CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG 9 0.22499999999999998 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 8 0.2 No Hit ATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGT 8 0.2 No Hit CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT 8 0.2 No Hit CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT 8 0.2 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 8 0.2 No Hit AAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGT 8 0.2 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 8 0.2 No Hit GTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTT 7 0.17500000000000002 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 7 0.17500000000000002 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 7 0.17500000000000002 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 7 0.17500000000000002 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 7 0.17500000000000002 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 7 0.17500000000000002 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 7 0.17500000000000002 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 6 0.15 No Hit CTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCC 6 0.15 No Hit GATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACT 6 0.15 No Hit GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT 6 0.15 No Hit GTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCA 6 0.15 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC 6 0.15 No Hit GTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTC 6 0.15 No Hit CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT 6 0.15 No Hit ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC 6 0.15 No Hit GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTT 6 0.15 No Hit GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG 6 0.15 No Hit AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC 6 0.15 No Hit GACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC 5 0.125 No Hit GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC 5 0.125 No Hit CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT 5 0.125 No Hit ATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGC 5 0.125 No Hit GCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA 5 0.125 No Hit AGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTT 5 0.125 No Hit GGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCT 5 0.125 No Hit CTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG 5 0.125 No Hit CTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATTAACCA 5 0.125 No Hit GTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGG 5 0.125 No Hit CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG 5 0.125 No Hit GGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGAC 5 0.125 No Hit GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGC 5 0.125 No Hit GGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAAC 5 0.125 No Hit AGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTT 5 0.125 No Hit GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA 5 0.125 No Hit CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG 5 0.125 No Hit AAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA 5 0.125 No Hit AGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAA 5 0.125 No Hit ACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA 5 0.125 No Hit ATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGT 5 0.125 No Hit AGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCA 5 0.125 No Hit GGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGC 5 0.125 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 5 0.125 No Hit GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC 5 0.125 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 5 0.125 No Hit GAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGG 5 0.125 No Hit CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT 5 0.125 No Hit CCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.037500000000000006 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.0875 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2125 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.425 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.7250000000000001 0.0 0.0 0.0 0.0 90-91 0.875 0.0 0.0 0.0 0.0 92-93 1.1124999999999998 0.0 0.0 0.0 0.0 94-95 1.4 0.0 0.0 0.0 0.0 96-97 1.65 0.0 0.0 0.0 0.0 98-99 1.9125 0.0 0.0 0.0 0.0 100-101 2.2625 0.0 0.0 0.0 0.0 102-103 2.5875 0.0 0.0 0.0 0.0 104-105 2.8625 0.0 0.0 0.0 0.0 106-107 3.2625 0.0 0.0 0.0 0.0 108-109 3.6125 0.0 0.0 0.0 0.0 110-111 3.9375 0.0 0.0 0.0 0.0 112-113 4.3375 0.0 0.0 0.0 0.0 114-115 4.95 0.0 0.0 0.0 0.0 116-117 5.6 0.0 0.0 0.0 0.0 118-119 6.1 0.0 0.0 0.0 0.0 120-121 6.6375 0.0 0.0 0.0 0.0 122-123 7.4125 0.0 0.0 0.0 0.0 124-125 8.025 0.0 0.0 0.0 0.0 126-127 8.5375 0.0 0.0 0.0 0.0 128-129 8.925 0.0 0.0 0.0 0.0 130-131 9.5875 0.0 0.0 0.0 0.0 132-133 10.3 0.0 0.0 0.0 0.0 134-135 11.075 0.0 0.0 0.0 0.0 136-137 12.0 0.0 0.0 0.0 0.0 138-139 12.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCCA 10 0.006830828 145.0 8 GGAGACT 10 0.006830828 145.0 4 AAAGGAG 10 0.006830828 145.0 1 GACTGCC 10 0.006830828 145.0 7 CTGCCAG 10 0.006830828 145.0 9 AGACTGC 10 0.006830828 145.0 6 GAGACTG 10 0.006830828 145.0 5 >>END_MODULE Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931486 spots for SRR7473351.sra Written 931486 spots for SRR7473351.sra Read 931495 spots for SRR7473351.sra Written 931495 spots for SRR7473351.sra SRR ids: ['SRR7473351.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_qxfcr5t1 SRR7473351.sra spots: 18629729 blocks: [[1, 931486], [931487, 1862972], [1862973, 2794458], [2794459, 3725944], [3725945, 4657430], [4657431, 5588916], [5588917, 6520402], [6520403, 7451888], [7451889, 8383374], [8383375, 9314860], [9314861, 10246346], [10246347, 11177832], [11177833, 12109318], [12109319, 13040804], [13040805, 13972290], [13972291, 14903776], [14903777, 15835262], [15835263, 16766748], [16766749, 17698234], [17698235, 18629729]] SRR7473351 file size 6291303 SRR7473351 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473351 SRR7473351_1.fastq SRR7473351_2.fastq Input file: SRR7473351_1.fastq Paired file: SRR7473351_2.fastq trimmed: SRR7473351-trimmed-pair1.fastq, SRR7473351-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 14:32:07 2024 >> started Sat Dec 7 14:32:38 2024 >> done (30.988s) 18629729 read pairs processed; of these: 48793 ( 0.26%) short read pairs filtered out after trimming by size control 221770 ( 1.19%) empty read pairs filtered out after trimming by size control 18359166 (98.55%) read pairs available; of these: 9492894 (51.71%) trimmed read pairs available after processing 8866272 (48.29%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 11 0.00% 20 6 0.00% 21 12 0.00% 22 18 0.00% 23 24 0.00% 24 29 0.00% 25 21 0.00% 26 26 0.00% 27 33 0.00% 28 31 0.00% 29 33 0.00% 30 44 0.00% 31 52 0.00% 32 41 0.00% 33 64 0.00% 34 45 0.00% 35 73 0.00% 36 68 0.00% 37 65 0.00% 38 81 0.00% 39 99 0.00% 40 127 0.00% 41 135 0.00% 42 146 0.00% 43 158 0.00% 44 213 0.00% 45 307 0.00% 46 243 0.00% 47 308 0.00% 48 292 0.00% 49 350 0.00% 50 335 0.00% 51 443 0.00% 52 478 0.00% 53 467 0.00% 54 544 0.00% 55 485 0.00% 56 591 0.00% 57 538 0.00% 58 659 0.00% 59 718 0.00% 60 837 0.00% 61 965 0.01% 62 1041 0.01% 63 1296 0.01% 64 1621 0.01% 65 3626 0.02% 66 3807 0.02% 67 3732 0.02% 68 5194 0.03% 69 16617 0.09% 70 27861 0.15% 71 13640 0.07% 72 7236 0.04% 73 5940 0.03% 74 5297 0.03% 75 4864 0.03% 76 4657 0.03% 77 4980 0.03% 78 5374 0.03% 79 6175 0.03% 80 6767 0.04% 81 7482 0.04% 82 8883 0.05% 83 11041 0.06% 84 14932 0.08% 85 15723 0.09% 86 16840 0.09% 87 17632 0.10% 88 20120 0.11% 89 19439 0.11% 90 21633 0.12% 91 23522 0.13% 92 22915 0.12% 93 28962 0.16% 94 29497 0.16% 95 33088 0.18% 96 31602 0.17% 97 31162 0.17% 98 29692 0.16% 99 31300 0.17% 100 35852 0.20% 101 34001 0.19% 102 37424 0.20% 103 39645 0.22% 104 43298 0.24% 105 48882 0.27% 106 45623 0.25% 107 43044 0.23% 108 47424 0.26% 109 57206 0.31% 110 57983 0.32% 111 50348 0.27% 112 53602 0.29% 113 69051 0.38% 114 61534 0.34% 115 67889 0.37% 116 68673 0.37% 117 61872 0.34% 118 63808 0.35% 119 62783 0.34% 120 67114 0.37% 121 62853 0.34% 122 69820 0.38% 123 76479 0.42% 124 78801 0.43% 125 78368 0.43% 126 75183 0.41% 127 75111 0.41% 128 73431 0.40% 129 76854 0.42% 130 80914 0.44% 131 80327 0.44% 132 84080 0.46% 133 89236 0.49% 134 96816 0.53% 135 102759 0.56% 136 102098 0.56% 137 110461 0.60% 138 109340 0.60% 139 110092 0.60% 140 109535 0.60% 141 122821 0.67% 142 124426 0.68% 143 133780 0.73% 144 147965 0.81% 145 166928 0.91% 146 196559 1.07% 147 241566 1.32% 148 350235 1.91% 149 694050 3.78% 150 3899541 21.24% 151 8866272 48.29% 18359166 reads passed initial QC criterion=sequence-density sequence-density=0.96 sequence-density-rank=1 fanout-score=5.35 fanout-score-rank=16 prefix-density=2.82 prefix-fanout=1.8 sequence=TCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGG criterion=fanout-score sequence-density=0.17 sequence-density-rank=29 fanout-score=29.47 fanout-score-rank=1 prefix-density=2.64 prefix-fanout=1.8 sequence=TTCGTTTTTTTTCTTG criterion=sequence-density sequence-density=4.27 sequence-density-rank=1 fanout-score=2.35 fanout-score-rank=30 prefix-density=4.57 prefix-fanout=2.2 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.28 sequence-density-rank=19 fanout-score=18.45 fanout-score-rank=1 prefix-density=4.46 prefix-fanout=1.1 sequence=AACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAGCGATTCAGCGCTATTTGCGCCAAGAACTGTTAACGTCTTGAATTCTGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGG -y GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG -o SRR7473351 SRR7473351_1.fastq SRR7473351_2.fastq Input file: SRR7473351_1.fastq Paired file: SRR7473351_2.fastq trimmed: SRR7473351-trimmed-pair1.fastq, SRR7473351-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCT -- paired 3' end adapter sequence (-y): GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACT -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 14:34:54 2024 >> started Sat Dec 7 14:35:05 2024 >> done (11.057s) 6119722 read pairs processed; of these: 74 ( 0.00%) short read pairs filtered out after trimming by size control 1071 ( 0.02%) empty read pairs filtered out after trimming by size control 6118577 (99.98%) read pairs available; of these: 1619 ( 0.03%) trimmed read pairs available after processing 6116958 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 7 0.00% 20 2 0.00% 21 8 0.00% 22 15 0.00% 23 13 0.00% 24 15 0.00% 25 15 0.00% 26 13 0.00% 27 22 0.00% 28 18 0.00% 29 16 0.00% 30 13 0.00% 31 19 0.00% 32 12 0.00% 33 21 0.00% 34 19 0.00% 35 17 0.00% 36 26 0.00% 37 24 0.00% 38 27 0.00% 39 37 0.00% 40 47 0.00% 41 57 0.00% 42 54 0.00% 43 59 0.00% 44 67 0.00% 45 103 0.00% 46 78 0.00% 47 112 0.00% 48 109 0.00% 49 128 0.00% 50 98 0.00% 51 152 0.00% 52 159 0.00% 53 156 0.00% 54 185 0.00% 55 171 0.00% 56 193 0.00% 57 179 0.00% 58 203 0.00% 59 239 0.00% 60 279 0.00% 61 321 0.01% 62 375 0.01% 63 469 0.01% 64 574 0.01% 65 1184 0.02% 66 1263 0.02% 67 1269 0.02% 68 1744 0.03% 69 5541 0.09% 70 9372 0.15% 71 4495 0.07% 72 2469 0.04% 73 1982 0.03% 74 1757 0.03% 75 1607 0.03% 76 1537 0.03% 77 1671 0.03% 78 1809 0.03% 79 2049 0.03% 80 2211 0.04% 81 2585 0.04% 82 2968 0.05% 83 3739 0.06% 84 4949 0.08% 85 5201 0.09% 86 5670 0.09% 87 5925 0.10% 88 6691 0.11% 89 6331 0.10% 90 7343 0.12% 91 7930 0.13% 92 7645 0.12% 93 9698 0.16% 94 10028 0.16% 95 10932 0.18% 96 10628 0.17% 97 10338 0.17% 98 9925 0.16% 99 10325 0.17% 100 11968 0.20% 101 11400 0.19% 102 12437 0.20% 103 13295 0.22% 104 14346 0.23% 105 16172 0.26% 106 15104 0.25% 107 14293 0.23% 108 15931 0.26% 109 19053 0.31% 110 19350 0.32% 111 16881 0.28% 112 17846 0.29% 113 23227 0.38% 114 20448 0.33% 115 22456 0.37% 116 23015 0.38% 117 20792 0.34% 118 21288 0.35% 119 20922 0.34% 120 22270 0.36% 121 20736 0.34% 122 23183 0.38% 123 25495 0.42% 124 26331 0.43% 125 26258 0.43% 126 24863 0.41% 127 24744 0.40% 128 24463 0.40% 129 25603 0.42% 130 26744 0.44% 131 26712 0.44% 132 28059 0.46% 133 29555 0.48% 134 32263 0.53% 135 34064 0.56% 136 34043 0.56% 137 37056 0.61% 138 36409 0.60% 139 36589 0.60% 140 36624 0.60% 141 41150 0.67% 142 41544 0.68% 143 44724 0.73% 144 49307 0.81% 145 55567 0.91% 146 65319 1.07% 147 80520 1.32% 148 116584 1.91% 149 232247 3.80% 150 1299364 21.24% 151 2954222 48.28% criterion=sequence-density sequence-density=0.97 sequence-density-rank=1 fanout-score=5.31 fanout-score-rank=16 prefix-density=2.83 prefix-fanout=1.8 sequence=TCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGG criterion=fanout-score sequence-density=0.17 sequence-density-rank=27 fanout-score=28.94 fanout-score-rank=1 prefix-density=2.71 prefix-fanout=1.8 sequence=TTCGTTTTTTTTCTTG criterion=sequence-density sequence-density=4.14 sequence-density-rank=1 fanout-score=2.34 fanout-score-rank=30 prefix-density=4.45 prefix-fanout=2.2 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=111.81 fanout-score-rank=1 prefix-density=0.59 prefix-fanout=1.0 sequence=TTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTTGGTGTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCCTATTGTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGAATTTGTGGAGTGAGAGGATTAAAGATAGGGAAGTTGTTGAAGTTATTGGCTGTGAGAGAGTGCCATTGATGAAACGT SRR7473351 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 14:37:35 Started mapping on | Dec 07 14:37:35 Finished on | Dec 07 15:24:33 Mapping speed, Million of reads per hour | 23.45 Number of input reads | 18358021 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 2175321 Uniquely mapped reads % | 11.85% Average mapped length | 286.93 Number of splices: Total | 1224604 Number of splices: Annotated (sjdb) | 1134784 Number of splices: GT/AG | 1206778 Number of splices: GC/AG | 15063 Number of splices: AT/AC | 681 Number of splices: Non-canonical | 2082 Mismatch rate per base, % | 0.11% Deletion rate per base | 0.01% Deletion average length | 1.46 Insertion rate per base | 0.01% Insertion average length | 1.20 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 55509 % of reads mapped to multiple loci | 0.30% Number of reads mapped to too many loci | 9247 % of reads mapped to too many loci | 0.05% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 84.88% % of reads unmapped: other | 2.92% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 16130980 16130980 16130980 N_multimapping 55509 55509 55509 N_noFeature 81375 2093182 100381 N_ambiguous 69000 409 6107 UnstrandedReadsAssigned:2024946 PositiveStrandReadsAssigned:81730 NegativeStrandReadsAssigned:2068833 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=144 echo kmer=139 SRR7473351 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473351-trimmed-pair1.fastq SRR7473351-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,358,021 reads, 2,218,277 reads pseudoaligned [quant] estimated average fragment length: 205.534 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,196 rounds 52973 SRR7473351.ke.tsv 35125 SRR7473351.se.tsv 88098 total ==> SRR7473351.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 731.595 0 0 PNS24247 1044 839.466 0 0 PNS24249 1928 1723.47 0 0 PNS24246 1044 839.466 0 0 PNS24248 1044 839.466 0 0 PNS24244 1471 1266.47 43 20.8879 PNS24243 293 114.609 0 0 KQK14069 1603 1398.47 18 7.91847 KQK14071 474 276.308 0 0 ==> SRR7473351.se.tsv <== BRADI_1g14170v3 17 BRADI_1g53295v3 0 BRADI_1g59795v3 75 BRADI_1g07683v3 0 BRADI_1g00485v3 7 BRADI_1g20270v3 94 BRADI_1g74790v3 18 BRADI_1g09890v3 0 BRADI_1g77505v3 37 BRADI_1g48960v3 0 SRR7473351 completed mapping pipeline successfully