Starting /dee2/code/volunteer_pipeline.sh SRR7473353 current disk space = 1542975660032 free memory = 1596603952 SRR7473353 SRAfilesize 5da7ecd3c2d5dcf8d6bd01c5cd5fe28e SRR7473353.sra SRR7473353.sra file validated SRR7473353 is paired end SRR7473353 is conventional basespace SRR7473353 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473353_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.554 34.0 34.0 34.0 33.0 34.0 2 33.583 34.0 34.0 34.0 33.0 34.0 3 33.59825 34.0 34.0 34.0 33.0 34.0 4 33.5895 34.0 34.0 34.0 33.0 34.0 5 33.606 34.0 34.0 34.0 33.0 34.0 6 37.3925 38.0 38.0 38.0 37.0 38.0 7 37.6185 38.0 38.0 38.0 38.0 38.0 8 37.5885 38.0 38.0 38.0 38.0 38.0 9 37.61675 38.0 38.0 38.0 38.0 38.0 10-14 37.5929 38.0 38.0 38.0 38.0 38.0 15-19 37.67405 38.0 38.0 38.0 38.0 38.0 20-24 37.6627 38.0 38.0 38.0 38.0 38.0 25-29 37.5746 38.0 38.0 38.0 38.0 38.0 30-34 37.397999999999996 38.0 38.0 38.0 37.4 38.0 35-39 37.50495 38.0 38.0 38.0 38.0 38.0 40-44 37.2211 38.0 38.0 38.0 37.2 38.0 45-49 37.337 38.0 38.0 38.0 37.4 38.0 50-54 37.3173 38.0 38.0 38.0 37.4 38.0 55-59 37.1871 38.0 38.0 38.0 36.8 38.0 60-64 37.33 38.0 38.0 38.0 37.2 38.0 65-69 37.15075 38.0 38.0 38.0 36.8 38.0 70-74 37.26225 38.0 38.0 38.0 37.2 38.0 75-79 37.043600000000005 38.0 38.0 38.0 37.0 38.0 80-84 36.837450000000004 38.0 38.0 38.0 36.6 38.0 85-89 36.88725 38.0 38.0 38.0 37.0 38.0 90-94 36.832 38.0 38.0 38.0 36.8 38.0 95-99 36.761849999999995 38.0 38.0 38.0 36.0 38.0 100-104 36.68685 38.0 38.0 38.0 35.8 38.0 105-109 36.4876 38.0 38.0 38.0 35.2 38.0 110-114 36.4452 38.0 38.0 38.0 35.0 38.0 115-119 36.33455 38.0 38.0 38.0 34.8 38.0 120-124 36.19325 38.0 38.0 38.0 34.4 38.0 125-129 35.8502 38.0 38.0 38.0 33.2 38.0 130-134 35.70795 38.0 37.6 38.0 32.8 38.0 135-139 35.3497 38.0 36.8 38.0 30.8 38.0 140-144 35.272099999999995 38.0 36.0 38.0 31.0 38.0 145-149 34.54485 38.0 35.6 38.0 28.0 38.0 150-151 31.565875000000002 36.5 31.5 38.0 15.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 2.0 8 2.0 9 2.0 10 0.0 11 0.0 12 3.0 13 1.0 14 4.0 15 4.0 16 2.0 17 5.0 18 13.0 19 15.0 20 3.0 21 8.0 22 4.0 23 4.0 24 5.0 25 5.0 26 10.0 27 11.0 28 18.0 29 26.0 30 36.0 31 30.0 32 39.0 33 55.0 34 87.0 35 147.0 36 400.0 37 3058.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.75 10.7 12.75 42.8 2 24.4994994994995 13.463463463463462 33.133133133133136 28.903903903903906 3 22.025 17.299999999999997 27.950000000000003 32.725 4 24.0 25.8 23.025000000000002 27.175 5 26.950000000000003 27.750000000000004 25.35 19.950000000000003 6 19.7 28.675 30.2 21.425 7 14.075 19.7 46.300000000000004 19.925 8 17.474999999999998 21.45 32.35 28.725 9 18.7 21.2 33.85 26.25 10-14 21.485000000000003 25.240000000000002 26.395000000000003 26.88 15-19 20.68 25.240000000000002 27.965 26.115 20-24 21.675 25.585 26.965 25.775 25-29 20.145 26.21 28.244999999999997 25.4 30-34 20.195 25.845000000000002 26.87 27.089999999999996 35-39 21.01 25.16 28.035 25.795 40-44 21.95 25.369999999999997 26.86 25.82 45-49 22.06 25.314999999999998 27.445000000000004 25.180000000000003 50-54 22.46 26.16 26.119999999999997 25.259999999999998 55-59 21.08 25.555 27.224999999999998 26.14 60-64 20.405 25.119999999999997 28.23 26.245 65-69 19.525000000000002 25.765 28.15 26.56 70-74 21.19 26.41 26.534999999999997 25.865 75-79 20.505000000000003 27.125 25.335 27.034999999999997 80-84 21.385 26.38 26.950000000000003 25.285000000000004 85-89 21.005 26.8 25.555 26.640000000000004 90-94 21.584999999999997 25.335 26.784999999999997 26.295 95-99 21.365000000000002 25.97 26.665 26.0 100-104 22.17 25.814999999999998 26.255 25.759999999999998 105-109 20.925 26.44 26.135 26.5 110-114 21.16211621162116 26.007600760076006 25.617561756175615 27.21272127212721 115-119 21.335 26.955000000000002 25.230000000000004 26.479999999999997 120-124 21.305 27.065 25.3 26.33 125-129 22.775000000000002 25.215 26.924999999999997 25.085 130-134 22.220000000000002 26.71 25.805 25.264999999999997 135-139 21.345 26.484999999999996 27.150000000000002 25.019999999999996 140-144 22.575 25.974999999999998 25.259999999999998 26.19 145-149 22.927513015618743 26.426712054465355 25.230276331597917 25.41549859831798 150-151 21.8625 25.55 26.0125 26.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.5 3 2.0 4 2.0 5 2.0 6 2.0 7 0.5 8 0.0 9 1.5 10 1.5 11 0.5 12 0.5 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.5 19 0.5 20 0.0 21 0.5 22 1.5 23 3.0 24 2.0 25 0.5 26 1.0 27 3.5 28 7.0 29 15.0 30 24.5 31 30.0 32 39.5 33 53.0 34 70.5 35 82.0 36 102.0 37 134.5 38 144.0 39 121.5 40 107.0 41 111.5 42 90.5 43 79.5 44 111.0 45 118.0 46 104.5 47 103.0 48 121.0 49 136.5 50 151.5 51 160.5 52 187.0 53 258.0 54 282.0 55 248.5 56 182.0 57 144.0 58 132.0 59 105.5 60 75.0 61 52.0 62 43.0 63 27.5 64 11.5 65 2.0 66 0.5 67 0.5 68 0.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.01 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.12 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.625 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67539267015707 58.5 2 10.12216404886562 14.499999999999998 3 3.9441535776614307 8.475000000000001 4 1.6055846422338569 4.6 5 1.0122164048865618 3.6249999999999996 6 0.6631762652705061 2.85 7 0.24432809773123912 1.225 8 0.24432809773123912 1.4000000000000001 9 0.13961605584642234 0.8999999999999999 >10 0.34904013961605584 3.925 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC 25 0.625 TruSeq Adapter, Index 21 (98% over 50bp) GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 23 0.575 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 19 0.475 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 18 0.44999999999999996 No Hit CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 17 0.42500000000000004 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 12 0.3 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 12 0.3 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 11 0.27499999999999997 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 10 0.25 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 10 0.25 No Hit CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT 9 0.22499999999999998 No Hit GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT 9 0.22499999999999998 No Hit CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG 9 0.22499999999999998 No Hit CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT 9 0.22499999999999998 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 8 0.2 No Hit CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT 8 0.2 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 8 0.2 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 8 0.2 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 8 0.2 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 8 0.2 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 8 0.2 No Hit GTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAG 7 0.17500000000000002 No Hit GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC 7 0.17500000000000002 No Hit CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC 7 0.17500000000000002 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 7 0.17500000000000002 No Hit GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG 7 0.17500000000000002 No Hit GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG 7 0.17500000000000002 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 7 0.17500000000000002 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 6 0.15 No Hit TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG 6 0.15 No Hit GTTTGATTGGCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAA 6 0.15 No Hit CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT 6 0.15 No Hit CGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTG 6 0.15 No Hit CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA 6 0.15 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA 6 0.15 No Hit TCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTC 6 0.15 No Hit ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA 6 0.15 No Hit CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT 6 0.15 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 6 0.15 No Hit ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC 6 0.15 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 6 0.15 No Hit GGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC 6 0.15 No Hit GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC 6 0.15 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 6 0.15 No Hit GTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATC 6 0.15 No Hit CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC 6 0.15 No Hit GGCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAAAATTAGTC 6 0.15 No Hit CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG 5 0.125 No Hit CTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGG 5 0.125 No Hit CCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCC 5 0.125 No Hit GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC 5 0.125 No Hit CCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAA 5 0.125 No Hit CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA 5 0.125 No Hit CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG 5 0.125 No Hit CCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 5 0.125 No Hit CCTATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTAC 5 0.125 No Hit GCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGG 5 0.125 No Hit CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG 5 0.125 No Hit TGGTGATTTAACCTTTTATCCCTTATTAGAAAAAGTGGCAAAAACAGGCA 5 0.125 No Hit GTCAATTTAAACGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGT 5 0.125 No Hit GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG 5 0.125 No Hit GCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCT 5 0.125 No Hit CGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCT 5 0.125 No Hit GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC 5 0.125 No Hit CTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 5 0.125 No Hit GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGA 5 0.125 No Hit CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT 5 0.125 No Hit GCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTT 5 0.125 No Hit GCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCC 5 0.125 No Hit CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC 5 0.125 No Hit CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC 5 0.125 No Hit CGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAG 5 0.125 No Hit CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG 5 0.125 No Hit AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG 5 0.125 No Hit GTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 5 0.125 No Hit CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.0625 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.1125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.1375 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.48750000000000004 0.0 0.0 0.0 0.0 82-83 0.6000000000000001 0.0 0.0 0.0 0.0 84-85 0.75 0.0 0.0 0.0 0.0 86-87 1.0875 0.0 0.0 0.0 0.0 88-89 1.4 0.0 0.0 0.0 0.0 90-91 1.7625000000000002 0.0 0.0 0.0 0.0 92-93 2.075 0.0 0.0 0.0 0.0 94-95 2.5250000000000004 0.0 0.0 0.0 0.0 96-97 3.2125 0.0 0.0 0.0 0.0 98-99 3.7874999999999996 0.0 0.0 0.0 0.0 100-101 4.425 0.0 0.0 0.0 0.0 102-103 5.025 0.0 0.0 0.0 0.0 104-105 5.6 0.0 0.0 0.0 0.0 106-107 6.325 0.0 0.0 0.0 0.0 108-109 6.925000000000001 0.0 0.0 0.0 0.0 110-111 7.612500000000001 0.0 0.0 0.0 0.0 112-113 8.0 0.0 0.0 0.0 0.0 114-115 8.7 0.0 0.0 0.0 0.0 116-117 9.8125 0.0 0.0 0.0 0.0 118-119 10.725 0.0 0.0 0.0 0.0 120-121 11.3625 0.0 0.0 0.0 0.0 122-123 12.2375 0.0 0.0 0.0 0.0 124-125 13.1375 0.0 0.0 0.0 0.0 126-127 13.9625 0.0 0.0 0.0 0.0 128-129 14.7875 0.0 0.0 0.0 0.0 130-131 15.8 0.0 0.0 0.0 0.0 132-133 16.875 0.0 0.0 0.0 0.0 134-135 17.875 0.0 0.0 0.0 0.0 136-137 18.9375 0.0 0.0 0.0 0.0 138-139 20.1625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR7473353 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473353_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.184 34.0 33.0 34.0 33.0 34.0 2 33.255 34.0 33.0 34.0 33.0 34.0 3 33.1765 34.0 33.0 34.0 33.0 34.0 4 33.14925 34.0 33.0 34.0 33.0 34.0 5 33.23325 34.0 33.0 34.0 33.0 34.0 6 37.27875 38.0 38.0 38.0 38.0 38.0 7 37.09775 38.0 38.0 38.0 37.0 38.0 8 37.1875 38.0 38.0 38.0 37.0 38.0 9 37.33425 38.0 38.0 38.0 38.0 38.0 10-14 37.188649999999996 38.0 38.0 38.0 37.6 38.0 15-19 37.24595 38.0 38.0 38.0 38.0 38.0 20-24 37.257549999999995 38.0 38.0 38.0 38.0 38.0 25-29 37.2724 38.0 38.0 38.0 38.0 38.0 30-34 37.349399999999996 38.0 38.0 38.0 38.0 38.0 35-39 37.118300000000005 38.0 38.0 38.0 37.4 38.0 40-44 37.2059 38.0 38.0 38.0 37.6 38.0 45-49 37.24345 38.0 38.0 38.0 38.0 38.0 50-54 37.30024999999999 38.0 38.0 38.0 38.0 38.0 55-59 37.332750000000004 38.0 38.0 38.0 38.0 38.0 60-64 37.259249999999994 38.0 38.0 38.0 37.8 38.0 65-69 37.032799999999995 38.0 38.0 38.0 37.0 38.0 70-74 36.9063 38.0 38.0 38.0 37.0 38.0 75-79 36.924499999999995 38.0 38.0 38.0 37.0 38.0 80-84 36.84115 38.0 38.0 38.0 37.0 38.0 85-89 36.7797 38.0 38.0 38.0 36.8 38.0 90-94 36.631099999999996 38.0 38.0 38.0 36.0 38.0 95-99 36.65065 38.0 38.0 38.0 35.8 38.0 100-104 36.563 38.0 38.0 38.0 35.0 38.0 105-109 36.4207 38.0 38.0 38.0 35.0 38.0 110-114 36.153949999999995 38.0 38.0 38.0 34.0 38.0 115-119 35.76665 38.0 37.4 38.0 32.8 38.0 120-124 35.9545 38.0 38.0 38.0 34.0 38.0 125-129 35.75575 38.0 37.8 38.0 33.0 38.0 130-134 35.3408 38.0 36.6 38.0 31.8 38.0 135-139 35.01055 38.0 36.0 38.0 29.8 38.0 140-144 34.4124 38.0 35.4 38.0 27.8 38.0 145-149 33.47555 38.0 33.6 38.0 20.4 38.0 150-151 28.770625 35.0 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 5.0 4 3.0 5 0.0 6 1.0 7 0.0 8 0.0 9 0.0 10 2.0 11 4.0 12 3.0 13 1.0 14 2.0 15 2.0 16 5.0 17 21.0 18 9.0 19 3.0 20 4.0 21 4.0 22 6.0 23 6.0 24 8.0 25 9.0 26 9.0 27 23.0 28 22.0 29 26.0 30 33.0 31 41.0 32 61.0 33 60.0 34 96.0 35 188.0 36 465.0 37 2871.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.0 16.975 13.65 31.374999999999996 2 30.855855855855857 23.273273273273272 27.75275275275275 18.11811811811812 3 26.69003505257887 25.338007010515774 26.86529794692038 21.106659989984976 4 27.93786018541719 33.70082686043598 19.443748433976445 18.917564520170384 5 29.894842263395095 32.924386579869804 19.45418127190786 17.72658988482724 6 26.625 35.525 19.3 18.55 7 22.85 19.725 34.599999999999994 22.825 8 25.63781890945473 23.311655827913956 24.512256128064035 26.538269134567283 9 27.1 24.6 24.65 23.65 10-14 27.442837844598987 26.53224595987392 22.589683294141192 23.4352329013859 15-19 27.85253364013806 26.161772797758992 23.760692311540193 22.225001250562755 20-24 28.29924488673301 26.298944841726257 23.94359153873081 21.45821873280992 25-29 26.531939372717723 27.132209494272423 24.84618078135161 21.489670351658248 30-34 27.82278227822782 27.457745774577457 23.932393239323932 20.787078707870787 35-39 27.796118447378955 25.8953581432573 24.519807923169267 21.788715486194477 40-44 28.613584075222565 26.182854856456938 25.14754426327898 20.056016805041512 45-49 26.92076830732293 26.525610244097642 25.90536214485794 20.64825930372149 50-54 26.140456182472988 25.985394157663066 26.720688275310124 21.15346138455382 55-59 26.077823347004102 26.74302290687206 26.57297189156747 20.606181854556365 60-64 25.66796757730411 27.299109376563596 26.008205744020813 21.02471730211148 65-69 26.17879667634398 26.969666633296622 26.228851736910602 20.622684953448793 70-74 26.47823911955978 27.028514257128567 25.697848924462228 20.795397698849424 75-79 25.36134033508377 27.22180545136284 25.811452863215806 21.605401350337583 80-84 26.185474189675872 27.230892356942775 25.81532613045218 20.76830732292917 85-89 26.415 27.04 25.535000000000004 21.01 90-94 26.083041520760382 27.35367683841921 25.797898949474735 20.765382691345675 95-99 26.31763176317632 28.492849284928496 25.107510751075107 20.08200820082008 100-104 26.745 28.105000000000004 24.775 20.375 105-109 26.927117202741236 28.918013105897657 23.93076884598069 20.22410084538042 110-114 27.15616548131824 28.6637283381749 23.725333066212563 20.4547731142943 115-119 28.07676889156144 29.459811585488072 23.762277009420725 18.701142513529764 120-124 26.593615530871613 29.320524367056937 23.821675172620836 20.264184929450614 125-129 27.224999999999998 29.585 23.849999999999998 19.34 130-134 27.22 28.835 23.985 19.96 135-139 27.985 28.139999999999997 24.03 19.845 140-144 28.395 27.815 24.445 19.345000000000002 145-149 28.74 27.92 23.830000000000002 19.509999999999998 150-151 29.5 28.5625 22.725 19.2125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.0 21 1.0 22 0.5 23 0.5 24 2.0 25 3.0 26 2.0 27 3.5 28 6.0 29 13.0 30 25.0 31 30.5 32 38.0 33 39.0 34 57.5 35 74.5 36 85.0 37 108.0 38 118.0 39 120.5 40 141.0 41 115.0 42 79.0 43 86.5 44 88.0 45 93.5 46 79.0 47 95.5 48 140.0 49 173.5 50 184.5 51 170.0 52 191.0 53 248.5 54 269.0 55 262.5 56 237.5 57 172.0 58 118.5 59 98.0 60 75.0 61 50.5 62 45.0 63 31.0 64 10.0 65 3.0 66 2.5 67 3.0 68 1.5 69 2.0 70 3.0 71 1.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.15 4 0.22499999999999998 5 0.15 6 0.0 7 0.0 8 0.05 9 0.0 10-14 0.065 15-19 0.045 20-24 0.015 25-29 0.045 30-34 0.01 35-39 0.04 40-44 0.03 45-49 0.04 50-54 0.04 55-59 0.03 60-64 0.06999999999999999 65-69 0.11 70-74 0.05 75-79 0.025 80-84 0.04 85-89 0.0 90-94 0.05 95-99 0.01 100-104 0.0 105-109 0.045 110-114 0.16999999999999998 115-119 0.22 120-124 0.06999999999999999 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 81.9327731092437 58.5 2 10.15406162464986 14.499999999999998 3 3.816526610644258 8.175 4 1.6456582633053223 4.7 5 0.700280112044818 2.5 6 0.49019607843137253 2.1 7 0.31512605042016806 1.575 8 0.350140056022409 2.0 9 0.21008403361344538 1.35 >10 0.3851540616246499 4.6 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 31 0.775 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 26 0.65 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 26 0.65 Illumina Single End PCR Primer 1 (100% over 50bp) GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 22 0.5499999999999999 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 13 0.325 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 13 0.325 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 11 0.27499999999999997 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 11 0.27499999999999997 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 11 0.27499999999999997 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 10 0.25 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 10 0.25 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 9 0.22499999999999998 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 9 0.22499999999999998 No Hit CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG 9 0.22499999999999998 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 9 0.22499999999999998 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 9 0.22499999999999998 No Hit CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT 9 0.22499999999999998 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 8 0.2 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 8 0.2 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 8 0.2 No Hit GGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCC 8 0.2 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 8 0.2 No Hit CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT 8 0.2 No Hit CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT 8 0.2 No Hit GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGC 8 0.2 No Hit CTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAAT 8 0.2 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 8 0.2 No Hit AATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCA 7 0.17500000000000002 No Hit CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAG 7 0.17500000000000002 No Hit GGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGA 7 0.17500000000000002 No Hit CAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCA 7 0.17500000000000002 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 7 0.17500000000000002 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 7 0.17500000000000002 No Hit CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT 7 0.17500000000000002 No Hit CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG 7 0.17500000000000002 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 7 0.17500000000000002 No Hit CTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAACAGCTT 6 0.15 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 6 0.15 No Hit AAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGT 6 0.15 No Hit GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA 6 0.15 No Hit TGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAG 6 0.15 No Hit CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA 6 0.15 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 6 0.15 No Hit CGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGA 6 0.15 No Hit TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC 6 0.15 No Hit GTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATC 6 0.15 No Hit GTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCC 6 0.15 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 6 0.15 No Hit CTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTTCA 6 0.15 No Hit TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC 6 0.15 No Hit GCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAAC 5 0.125 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 5 0.125 No Hit GTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTT 5 0.125 No Hit TGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTT 5 0.125 No Hit GGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATA 5 0.125 No Hit GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG 5 0.125 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 5 0.125 No Hit CCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT 5 0.125 No Hit GGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGA 5 0.125 No Hit GTATCTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATTA 5 0.125 No Hit GGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT 5 0.125 No Hit ATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCAT 5 0.125 No Hit GCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTCGTGAATTCATCTCC 5 0.125 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 5 0.125 No Hit TGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATA 5 0.125 No Hit GAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGA 5 0.125 No Hit CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT 5 0.125 No Hit CGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGG 5 0.125 No Hit GAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGG 5 0.125 No Hit CGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTGAGGCGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.0625 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.1125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.1375 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.48750000000000004 0.0 0.0 0.0 0.0 82-83 0.625 0.0 0.0 0.0 0.0 84-85 0.7749999999999999 0.0 0.0 0.0 0.0 86-87 1.1125 0.0 0.0 0.0 0.0 88-89 1.4249999999999998 0.0 0.0 0.0 0.0 90-91 1.7875 0.0 0.0 0.0 0.0 92-93 2.0999999999999996 0.0 0.0 0.0 0.0 94-95 2.55 0.0 0.0 0.0 0.0 96-97 3.2625 0.0 0.0 0.0 0.0 98-99 3.8875 0.0 0.0 0.0 0.0 100-101 4.5125 0.0 0.0 0.0 0.0 102-103 5.125 0.0 0.0 0.0 0.0 104-105 5.7 0.0 0.0 0.0 0.0 106-107 6.4125 0.0 0.0 0.0 0.0 108-109 7.025 0.0 0.0 0.0 0.0 110-111 7.7125 0.0 0.0 0.0 0.0 112-113 8.0875 0.0 0.0 0.0 0.0 114-115 8.775 0.0 0.0 0.0 0.0 116-117 9.912500000000001 0.0 0.0 0.0 0.0 118-119 10.825 0.0 0.0 0.0 0.0 120-121 11.4625 0.0 0.0 0.0 0.0 122-123 12.337499999999999 0.0 0.0 0.0 0.0 124-125 13.2625 0.0 0.0 0.0 0.0 126-127 14.0875 0.0 0.0 0.0 0.0 128-129 14.899999999999999 0.0 0.0 0.0 0.0 130-131 15.8875 0.0 0.0 0.0 0.0 132-133 16.8625 0.0 0.0 0.0 0.0 134-135 17.9625 0.0 0.0 0.0 0.0 136-137 19.05 0.0 0.0 0.0 0.0 138-139 20.2625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTCT 10 0.006830828 145.0 7 >>END_MODULE Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848918 spots for SRR7473353.sra Written 848918 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra Read 848904 spots for SRR7473353.sra Written 848904 spots for SRR7473353.sra SRR ids: ['SRR7473353.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_iu64pork SRR7473353.sra spots: 16978094 blocks: [[1, 848904], [848905, 1697808], [1697809, 2546712], [2546713, 3395616], [3395617, 4244520], [4244521, 5093424], [5093425, 5942328], [5942329, 6791232], [6791233, 7640136], [7640137, 8489040], [8489041, 9337944], [9337945, 10186848], [10186849, 11035752], [11035753, 11884656], [11884657, 12733560], [12733561, 13582464], [13582465, 14431368], [14431369, 15280272], [15280273, 16129176], [16129177, 16978094]] SRR7473353 file size 5731618 SRR7473353 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473353 SRR7473353_1.fastq SRR7473353_2.fastq Input file: SRR7473353_1.fastq Paired file: SRR7473353_2.fastq trimmed: SRR7473353-trimmed-pair1.fastq, SRR7473353-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 14:39:15 2024 >> started Sat Dec 7 14:39:35 2024 >> done (20.242s) 16978094 read pairs processed; of these: 19693 ( 0.12%) short read pairs filtered out after trimming by size control 143886 ( 0.85%) empty read pairs filtered out after trimming by size control 16814515 (99.04%) read pairs available; of these: 9126715 (54.28%) trimmed read pairs available after processing 7687800 (45.72%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 11 0.00% 20 5 0.00% 21 7 0.00% 22 10 0.00% 23 14 0.00% 24 10 0.00% 25 18 0.00% 26 26 0.00% 27 26 0.00% 28 28 0.00% 29 35 0.00% 30 43 0.00% 31 49 0.00% 32 64 0.00% 33 59 0.00% 34 68 0.00% 35 88 0.00% 36 89 0.00% 37 98 0.00% 38 112 0.00% 39 145 0.00% 40 152 0.00% 41 182 0.00% 42 231 0.00% 43 212 0.00% 44 233 0.00% 45 258 0.00% 46 309 0.00% 47 369 0.00% 48 408 0.00% 49 453 0.00% 50 528 0.00% 51 637 0.00% 52 699 0.00% 53 757 0.00% 54 777 0.00% 55 768 0.00% 56 851 0.01% 57 969 0.01% 58 1057 0.01% 59 1307 0.01% 60 1481 0.01% 61 1701 0.01% 62 1997 0.01% 63 2272 0.01% 64 2390 0.01% 65 3340 0.02% 66 3051 0.02% 67 3473 0.02% 68 4847 0.03% 69 13746 0.08% 70 15376 0.09% 71 8136 0.05% 72 7236 0.04% 73 7618 0.05% 74 7759 0.05% 75 7955 0.05% 76 7971 0.05% 77 8782 0.05% 78 9436 0.06% 79 11288 0.07% 80 11951 0.07% 81 13358 0.08% 82 14999 0.09% 83 18008 0.11% 84 22614 0.13% 85 23683 0.14% 86 25645 0.15% 87 27626 0.16% 88 31355 0.19% 89 31853 0.19% 90 34043 0.20% 91 34885 0.21% 92 35192 0.21% 93 43450 0.26% 94 43200 0.26% 95 49008 0.29% 96 47297 0.28% 97 49747 0.30% 98 47940 0.29% 99 48951 0.29% 100 54836 0.33% 101 50729 0.30% 102 54072 0.32% 103 55968 0.33% 104 60174 0.36% 105 66712 0.40% 106 62746 0.37% 107 60894 0.36% 108 65060 0.39% 109 73799 0.44% 110 77286 0.46% 111 67756 0.40% 112 69175 0.41% 113 83018 0.49% 114 76035 0.45% 115 82776 0.49% 116 85239 0.51% 117 77610 0.46% 118 78322 0.47% 119 78056 0.46% 120 81369 0.48% 121 75152 0.45% 122 83499 0.50% 123 89785 0.53% 124 88035 0.52% 125 87514 0.52% 126 86730 0.52% 127 88962 0.53% 128 85709 0.51% 129 87454 0.52% 130 91047 0.54% 131 90933 0.54% 132 93214 0.55% 133 96005 0.57% 134 104522 0.62% 135 105210 0.63% 136 102078 0.61% 137 112797 0.67% 138 113083 0.67% 139 113921 0.68% 140 110653 0.66% 141 121629 0.72% 142 123130 0.73% 143 129177 0.77% 144 138490 0.82% 145 152159 0.90% 146 171637 1.02% 147 206208 1.23% 148 287959 1.71% 149 550882 3.28% 150 3080678 18.32% 151 7687800 45.72% 16814515 reads passed initial QC criterion=sequence-density sequence-density=0.90 sequence-density-rank=1 fanout-score=4.49 fanout-score-rank=23 prefix-density=3.82 prefix-fanout=1.1 sequence=GATCGTCGCCTTGGTGAGCCGTT criterion=fanout-score sequence-density=0.17 sequence-density-rank=25 fanout-score=35.66 fanout-score-rank=1 prefix-density=4.58 prefix-fanout=1.4 sequence=CCCGAAGGCACCAATCCATCTCTGGAAAGTTCTGTGGATGTCAA criterion=sequence-density sequence-density=1.08 sequence-density-rank=1 fanout-score=3.16 fanout-score-rank=26 prefix-density=3.43 prefix-fanout=1.0 sequence=GGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=106.32 fanout-score-rank=1 prefix-density=0.60 prefix-fanout=1.0 sequence=TTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTTGGTGTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCCTATTGTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGAATTTGTGGAGTGAGAGGATTAAAGATAGGGAAGTTGTTGAAGTTATTGGCTGTGAGAGAGTGCCATTGATGAAA SRR7473353 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 14:41:49 Started mapping on | Dec 07 14:41:49 Finished on | Dec 07 15:19:02 Mapping speed, Million of reads per hour | 27.11 Number of input reads | 16814515 Average input read length | 283 UNIQUE READS: Uniquely mapped reads number | 3224889 Uniquely mapped reads % | 19.18% Average mapped length | 272.42 Number of splices: Total | 545682 Number of splices: Annotated (sjdb) | 468220 Number of splices: GT/AG | 534998 Number of splices: GC/AG | 7126 Number of splices: AT/AC | 190 Number of splices: Non-canonical | 3368 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.01% Deletion average length | 1.61 Insertion rate per base | 0.01% Insertion average length | 1.16 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 57149 % of reads mapped to multiple loci | 0.34% Number of reads mapped to too many loci | 10950 % of reads mapped to too many loci | 0.07% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 77.82% % of reads unmapped: other | 2.59% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 13535912 13535912 13535912 N_multimapping 57149 57149 57149 N_noFeature 129872 3069869 161605 N_ambiguous 132440 13288 13485 UnstrandedReadsAssigned:2962577 PositiveStrandReadsAssigned:141732 NegativeStrandReadsAssigned:3049799 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=134 echo kmer=129 SRR7473353 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473353-trimmed-pair1.fastq SRR7473353-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,814,515 reads, 3,204,958 reads pseudoaligned [quant] estimated average fragment length: 163.446 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,019 rounds 52973 SRR7473353.ke.tsv 35125 SRR7473353.se.tsv 88098 total ==> SRR7473353.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 773.569 0 0 PNS24247 1044 881.554 0 0 PNS24249 1928 1765.55 0 0 PNS24246 1044 881.554 0 0 PNS24248 1044 881.554 0 0 PNS24244 1471 1308.55 59 20.4966 PNS24243 293 136.547 0 0 KQK14069 1603 1440.55 42 13.2538 KQK14071 474 312.477 0 0 ==> SRR7473353.se.tsv <== BRADI_1g14170v3 43 BRADI_1g53295v3 0 BRADI_1g59795v3 73 BRADI_1g07683v3 0 BRADI_1g00485v3 5 BRADI_1g20270v3 141 BRADI_1g74790v3 29 BRADI_1g09890v3 0 BRADI_1g77505v3 54 BRADI_1g48960v3 0 SRR7473353 completed mapping pipeline successfully