Starting /dee2/code/volunteer_pipeline.sh SRR7473364 current disk space = 1542518116352 free memory = 1594979324 SRR7473364 SRAfilesize fe8537b99354fb796d7b26715d06d6b9 SRR7473364.sra SRR7473364.sra file validated SRR7473364 is paired end SRR7473364 is conventional basespace SRR7473364 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473364_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.528 34.0 34.0 34.0 33.0 34.0 2 33.56775 34.0 34.0 34.0 33.0 34.0 3 33.56375 34.0 34.0 34.0 33.0 34.0 4 33.5055 34.0 34.0 34.0 33.0 34.0 5 33.57425 34.0 34.0 34.0 33.0 34.0 6 37.318 38.0 38.0 38.0 36.0 38.0 7 37.58475 38.0 38.0 38.0 37.0 38.0 8 37.59075 38.0 38.0 38.0 38.0 38.0 9 37.64075 38.0 38.0 38.0 38.0 38.0 10-14 37.5991 38.0 38.0 38.0 38.0 38.0 15-19 37.63085 38.0 38.0 38.0 38.0 38.0 20-24 37.62065 38.0 38.0 38.0 38.0 38.0 25-29 37.580400000000004 38.0 38.0 38.0 38.0 38.0 30-34 37.47765 38.0 38.0 38.0 37.8 38.0 35-39 37.315000000000005 38.0 38.0 38.0 37.2 38.0 40-44 37.352549999999994 38.0 38.0 38.0 37.8 38.0 45-49 37.2952 38.0 38.0 38.0 37.2 38.0 50-54 37.30970000000001 38.0 38.0 38.0 37.2 38.0 55-59 37.114850000000004 38.0 38.0 38.0 36.6 38.0 60-64 37.01055 38.0 38.0 38.0 36.2 38.0 65-69 36.8118 38.0 38.0 38.0 35.4 38.0 70-74 37.1087 38.0 38.0 38.0 37.0 38.0 75-79 36.6713 38.0 38.0 38.0 36.6 38.0 80-84 36.52935 38.0 38.0 38.0 36.0 38.0 85-89 36.44855 38.0 38.0 38.0 35.8 38.0 90-94 36.4027 38.0 38.0 38.0 35.2 38.0 95-99 36.32275 38.0 38.0 38.0 35.0 38.0 100-104 36.1892 38.0 38.0 38.0 34.6 38.0 105-109 36.124399999999994 38.0 38.0 38.0 34.4 38.0 110-114 35.98785 38.0 38.0 38.0 34.0 38.0 115-119 35.88355 38.0 38.0 38.0 33.8 38.0 120-124 35.75645 38.0 38.0 38.0 33.2 38.0 125-129 35.26545 38.0 36.8 38.0 31.4 38.0 130-134 34.88615 38.0 35.8 38.0 28.6 38.0 135-139 34.5315 38.0 35.4 38.0 27.4 38.0 140-144 34.277750000000005 38.0 35.0 38.0 26.0 38.0 145-149 33.78885 38.0 34.2 38.0 23.0 38.0 150-151 29.41225 35.5 27.0 38.0 7.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 0.0 10 1.0 11 0.0 12 1.0 13 3.0 14 8.0 15 8.0 16 12.0 17 12.0 18 20.0 19 21.0 20 5.0 21 6.0 22 6.0 23 3.0 24 5.0 25 17.0 26 14.0 27 13.0 28 18.0 29 29.0 30 29.0 31 32.0 32 56.0 33 72.0 34 100.0 35 161.0 36 490.0 37 2855.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.875 9.8 10.625 40.699999999999996 2 22.8 13.375 30.425 33.4 3 22.2 15.6 25.124999999999996 37.075 4 24.8 24.525 21.325 29.349999999999998 5 28.4 26.974999999999998 23.025000000000002 21.6 6 19.875 27.825 28.425 23.875 7 16.475 20.4 41.4 21.725 8 18.55 19.325 30.5 31.624999999999996 9 18.65 19.725 32.625 28.999999999999996 10-14 22.065 23.47 24.23 30.235 15-19 22.54 22.205 26.365 28.89 20-24 22.759999999999998 23.715 25.314999999999998 28.21 25-29 21.63 23.27 25.765 29.335 30-34 21.36 23.56 25.245 29.835 35-39 21.385 23.65 26.834999999999997 28.13 40-44 22.68 22.5 26.195 28.625 45-49 22.975 22.745 25.86 28.42 50-54 22.759999999999998 23.265 25.145 28.83 55-59 21.735 21.365000000000002 27.3 29.599999999999998 60-64 22.045 21.7 27.744999999999997 28.51 65-69 21.07 23.32 25.509999999999998 30.099999999999998 70-74 21.75 25.035 24.295 28.92 75-79 22.43 25.419999999999998 23.625 28.525 80-84 21.29 24.92 25.05 28.74 85-89 22.6 23.799999999999997 23.745 29.854999999999997 90-94 22.915 22.11 25.009999999999998 29.965000000000003 95-99 22.830000000000002 23.815 24.7 28.655 100-104 23.26 24.855 23.49 28.395 105-109 22.58 24.27 23.935000000000002 29.215000000000003 110-114 22.67 24.81 23.62 28.9 115-119 22.770000000000003 24.47 23.935000000000002 28.825 120-124 21.975 24.775 23.43 29.82 125-129 23.47 23.165 24.884999999999998 28.48 130-134 22.762519385662113 25.39396668167492 23.88813847616189 27.955375456501073 135-139 22.920730182545636 24.391097774443608 24.28607151787947 28.402100525131285 140-144 23.665 24.575 22.705000000000002 29.054999999999996 145-149 23.265 24.060000000000002 24.165 28.51 150-151 22.825 24.462500000000002 24.224999999999998 28.487499999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 1.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 0.5 26 1.0 27 2.0 28 5.0 29 6.5 30 9.5 31 14.5 32 14.5 33 17.0 34 21.5 35 31.5 36 44.5 37 54.0 38 63.5 39 64.0 40 64.0 41 78.0 42 84.5 43 79.0 44 91.0 45 87.5 46 92.0 47 97.0 48 96.5 49 116.5 50 148.0 51 172.0 52 218.0 53 304.5 54 336.0 55 313.5 56 240.5 57 193.0 58 165.0 59 133.0 60 111.0 61 78.5 62 74.5 63 57.5 64 33.0 65 24.0 66 23.0 67 18.5 68 17.0 69 18.5 70 11.5 71 11.0 72 12.0 73 10.5 74 6.5 75 5.5 76 7.0 77 4.5 78 3.0 79 1.5 80 1.0 81 1.5 82 1.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.055 135-139 0.025 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.525 #Duplication Level Percentage of deduplicated Percentage of total 1 84.78341355053684 57.25 2 7.293594964827841 9.85 3 2.332469455757127 4.725 4 1.814142910033321 4.9 5 1.036653091447612 3.5000000000000004 6 0.6664198445020363 2.7 7 0.40725657164013324 1.925 8 0.1480932987782303 0.8 9 0.18511662347278787 1.125 >10 1.3328396890040726 13.225000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATGC 40 1.0 TruSeq Adapter, Index 25 (100% over 50bp) GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 28 0.7000000000000001 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATGCC 25 0.625 TruSeq Adapter, Index 25 (100% over 50bp) CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 22 0.5499999999999999 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 22 0.5499999999999999 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 19 0.475 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 18 0.44999999999999996 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 17 0.42500000000000004 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 16 0.4 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 15 0.375 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 15 0.375 No Hit CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA 14 0.35000000000000003 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 14 0.35000000000000003 No Hit CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 14 0.35000000000000003 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 14 0.35000000000000003 No Hit GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC 13 0.325 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 13 0.325 No Hit GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA 13 0.325 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC 13 0.325 No Hit GTTTGATTGGCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAA 12 0.3 No Hit CCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCC 12 0.3 No Hit CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA 12 0.3 No Hit TGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCA 12 0.3 No Hit GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT 12 0.3 No Hit CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAG 11 0.27499999999999997 No Hit CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC 11 0.27499999999999997 No Hit GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGA 11 0.27499999999999997 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 11 0.27499999999999997 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 10 0.25 No Hit CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC 10 0.25 No Hit CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT 10 0.25 No Hit CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT 10 0.25 No Hit GTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACT 10 0.25 No Hit CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT 10 0.25 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 10 0.25 No Hit CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC 10 0.25 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG 9 0.22499999999999998 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 9 0.22499999999999998 No Hit CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC 9 0.22499999999999998 No Hit GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC 9 0.22499999999999998 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 9 0.22499999999999998 No Hit GCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTT 8 0.2 No Hit AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA 8 0.2 No Hit GCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTG 8 0.2 No Hit GCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACAC 8 0.2 No Hit TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAG 7 0.17500000000000002 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 7 0.17500000000000002 No Hit CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG 7 0.17500000000000002 No Hit CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT 7 0.17500000000000002 No Hit GCCTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAACATTAGTCG 7 0.17500000000000002 No Hit TCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTC 7 0.17500000000000002 No Hit ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGA 7 0.17500000000000002 No Hit GGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTG 7 0.17500000000000002 No Hit GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC 7 0.17500000000000002 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATAACTCGTATGCC 7 0.17500000000000002 TruSeq Adapter, Index 25 (98% over 50bp) CACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGT 7 0.17500000000000002 No Hit CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG 6 0.15 No Hit CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT 6 0.15 No Hit GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTA 6 0.15 No Hit CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT 6 0.15 No Hit GGAAAACCAGCCTACACGCTTAAACCGGGACAACCGTCGCCCGGCCAACA 6 0.15 No Hit GCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGG 6 0.15 No Hit CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG 6 0.15 No Hit TCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGT 6 0.15 No Hit GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC 6 0.15 No Hit GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTA 6 0.15 No Hit CTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTC 6 0.15 No Hit GCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCT 6 0.15 No Hit GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG 6 0.15 No Hit GTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATC 6 0.15 No Hit CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT 6 0.15 No Hit CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC 6 0.15 No Hit CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA 6 0.15 No Hit CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG 6 0.15 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC 5 0.125 No Hit CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA 5 0.125 No Hit GCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAA 5 0.125 No Hit GTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCC 5 0.125 No Hit CGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGAT 5 0.125 No Hit GCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT 5 0.125 No Hit GGGAGAACCAGCTATCTCCCGGTTTGATTGGCCTTTCACCCCCAGCCACA 5 0.125 No Hit CCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAA 5 0.125 No Hit CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG 5 0.125 No Hit GCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGG 5 0.125 No Hit CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 5 0.125 No Hit GGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTAC 5 0.125 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA 5 0.125 No Hit ATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTC 5 0.125 No Hit GCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGC 5 0.125 No Hit GGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAG 5 0.125 No Hit CTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGC 5 0.125 No Hit CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT 5 0.125 No Hit GCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTA 5 0.125 No Hit GCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGA 5 0.125 No Hit CTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAG 5 0.125 No Hit CACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACC 5 0.125 No Hit CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT 5 0.125 No Hit CCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACA 5 0.125 No Hit CGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAG 5 0.125 No Hit GCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGG 5 0.125 No Hit GGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGA 5 0.125 No Hit GTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.1375 0.0 0.0 0.0 0.0 72-73 0.1875 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.25 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.4375 0.0 0.0 0.0 0.0 84-85 0.6625 0.0 0.0 0.0 0.0 86-87 0.8875 0.0 0.0 0.0 0.0 88-89 1.1125 0.0 0.0 0.0 0.0 90-91 1.3624999999999998 0.0 0.0 0.0 0.0 92-93 1.675 0.0 0.0 0.0 0.0 94-95 1.8875 0.0 0.0 0.0 0.0 96-97 2.2375 0.0 0.0 0.0 0.0 98-99 2.75 0.0 0.0 0.0 0.0 100-101 3.1875 0.0 0.0 0.0 0.0 102-103 3.6 0.0 0.0 0.0 0.0 104-105 4.275 0.0 0.0 0.0 0.0 106-107 4.9625 0.0 0.0 0.0 0.0 108-109 5.6 0.0 0.0 0.0 0.0 110-111 6.262499999999999 0.0 0.0 0.0 0.0 112-113 6.9125 0.0 0.0 0.0 0.0 114-115 7.5375 0.0 0.0 0.0 0.0 116-117 8.3375 0.0 0.0 0.0 0.0 118-119 9.0875 0.0 0.0 0.0 0.0 120-121 9.7875 0.0 0.0 0.0 0.0 122-123 10.4875 0.0 0.0 0.0 0.0 124-125 11.3625 0.0 0.0 0.0 0.0 126-127 12.175 0.0 0.0 0.0 0.0 128-129 13.1875 0.0 0.0 0.0 0.0 130-131 14.05 0.0 0.0 0.0 0.0 132-133 15.025 0.0 0.0 0.0 0.0 134-135 15.825 0.0 0.0 0.0 0.0 136-137 16.85 0.0 0.0 0.0 0.0 138-139 17.7875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCTTT 10 0.006830828 145.0 2 CGACGCT 10 0.006830828 145.0 145 TCTTTTC 10 0.006830828 145.0 4 ATTTCTT 10 0.006830828 145.0 1 >>END_MODULE SRR7473364 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473364_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.02425 34.0 33.0 34.0 32.0 34.0 2 33.077 34.0 33.0 34.0 33.0 34.0 3 32.975 34.0 33.0 34.0 33.0 34.0 4 32.78725 34.0 33.0 34.0 32.0 34.0 5 32.8815 34.0 33.0 34.0 33.0 34.0 6 36.3535 38.0 38.0 38.0 35.0 38.0 7 36.769 38.0 38.0 38.0 36.0 38.0 8 36.6585 38.0 38.0 38.0 37.0 38.0 9 36.80425 38.0 38.0 38.0 37.0 38.0 10-14 36.757600000000004 38.0 38.0 38.0 37.0 38.0 15-19 36.6973 38.0 38.0 38.0 36.8 38.0 20-24 36.6747 38.0 38.0 38.0 36.2 38.0 25-29 36.81965 38.0 38.0 38.0 37.4 38.0 30-34 36.79565 38.0 38.0 38.0 37.2 38.0 35-39 36.77165 38.0 38.0 38.0 37.2 38.0 40-44 36.763999999999996 38.0 38.0 38.0 37.0 38.0 45-49 36.59845 38.0 38.0 38.0 36.2 38.0 50-54 36.77315 38.0 38.0 38.0 37.0 38.0 55-59 36.8723 38.0 38.0 38.0 37.2 38.0 60-64 36.8658 38.0 38.0 38.0 37.2 38.0 65-69 36.6333 38.0 38.0 38.0 36.6 38.0 70-74 36.1579 38.0 38.0 38.0 36.0 38.0 75-79 36.1305 38.0 38.0 38.0 36.0 38.0 80-84 36.06095 38.0 38.0 38.0 35.4 38.0 85-89 35.95665 38.0 38.0 38.0 35.0 38.0 90-94 35.8219 38.0 38.0 38.0 34.4 38.0 95-99 35.7985 38.0 38.0 38.0 34.2 38.0 100-104 35.67015 38.0 38.0 38.0 34.0 38.0 105-109 35.5376 38.0 38.0 38.0 33.6 38.0 110-114 35.015750000000004 38.0 37.8 38.0 30.0 38.0 115-119 35.068599999999996 38.0 38.0 38.0 31.2 38.0 120-124 34.5852 38.0 36.4 38.0 26.4 38.0 125-129 34.468650000000004 38.0 36.0 38.0 27.4 38.0 130-134 34.2121 38.0 35.8 38.0 25.0 38.0 135-139 33.848400000000005 38.0 35.6 38.0 22.4 38.0 140-144 33.1697 38.0 34.0 38.0 15.4 38.0 145-149 32.14265 38.0 33.0 38.0 6.0 38.0 150-151 26.933625 33.0 17.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28.0 3 9.0 4 2.0 5 4.0 6 1.0 7 0.0 8 0.0 9 3.0 10 13.0 11 22.0 12 8.0 13 2.0 14 5.0 15 6.0 16 9.0 17 35.0 18 10.0 19 4.0 20 11.0 21 6.0 22 6.0 23 15.0 24 9.0 25 18.0 26 15.0 27 14.0 28 20.0 29 22.0 30 37.0 31 43.0 32 44.0 33 70.0 34 115.0 35 195.0 36 513.0 37 2686.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.175000000000004 17.224999999999998 11.325000000000001 28.275 2 31.324999999999996 20.4 24.125 24.15 3 28.264132066033014 23.836918459229615 22.911455727863935 24.987493746873437 4 32.4 29.125 18.05 20.424999999999997 5 33.58339584896224 30.707676919229808 15.228807201800452 20.4801200300075 6 26.424999999999997 35.05 17.325 21.2 7 23.849999999999998 19.650000000000002 32.5 24.0 8 29.049999999999997 22.975 19.400000000000002 28.575 9 28.999999999999996 23.974999999999998 22.8 24.224999999999998 10-14 30.205 24.945 19.99 24.86 15-19 31.175000000000004 24.224999999999998 21.365000000000002 23.235 20-24 31.569999999999997 24.715 20.82 22.895 25-29 29.335 26.395000000000003 21.535 22.735 30-34 30.43 26.700000000000003 20.974999999999998 21.895 35-39 30.585 24.25 22.195 22.97 40-44 31.755 24.779999999999998 22.0 21.465 45-49 30.445 24.560000000000002 22.415 22.58 50-54 28.975 25.695 22.785 22.545 55-59 29.104999999999997 25.295 23.865 21.735 60-64 28.92 25.665 22.33 23.085 65-69 30.375000000000004 26.355 21.89 21.38 70-74 30.14 26.334999999999997 22.015 21.51 75-79 28.675 26.625 22.005 22.695 80-84 30.385 25.445 22.58 21.59 85-89 29.555 25.97 22.075 22.400000000000002 90-94 30.145 25.490000000000002 21.875 22.49 95-99 28.865000000000002 26.72 22.495 21.92 100-104 30.044999999999998 26.14 22.6 21.215 105-109 28.725 27.465 21.115000000000002 22.695 110-114 30.256051210242045 27.460492098419685 21.039207841568313 21.244248849769953 115-119 30.083008300830084 27.872787278727873 21.847184718471844 20.1970197019702 120-124 29.549999999999997 26.534999999999997 22.005 21.91 125-129 30.48 26.745 22.31 20.465 130-134 29.985 26.445 21.58 21.990000000000002 135-139 31.240000000000002 26.419999999999998 20.935000000000002 21.404999999999998 140-144 30.435000000000002 26.784999999999997 21.16 21.62 145-149 31.380000000000003 27.250000000000004 20.885 20.485 150-151 32.15 27.8875 20.125 19.8375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.5 20 1.0 21 1.0 22 0.5 23 0.5 24 1.0 25 0.5 26 3.5 27 4.5 28 2.0 29 3.0 30 6.0 31 10.5 32 12.0 33 12.0 34 15.0 35 20.0 36 28.5 37 39.5 38 48.0 39 50.5 40 56.0 41 57.5 42 55.0 43 60.0 44 67.0 45 70.5 46 77.0 47 90.0 48 108.5 49 137.5 50 165.0 51 172.0 52 211.0 53 318.0 54 342.0 55 300.0 56 263.0 57 205.0 58 149.0 59 126.0 60 120.0 61 99.0 62 103.0 63 79.5 64 40.5 65 35.5 66 31.5 67 23.0 68 26.5 69 32.0 70 23.0 71 18.0 72 17.5 73 13.5 74 12.5 75 7.5 76 7.5 77 9.0 78 4.0 79 2.0 80 1.0 81 0.0 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.05 4 0.0 5 0.025 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.02 115-119 0.01 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.15 #Duplication Level Percentage of deduplicated Percentage of total 1 84.99648629655657 60.475 2 8.151791988756148 11.600000000000001 3 3.056921995783556 6.525 4 1.1595221363316937 3.3000000000000003 5 0.6324666198172875 2.25 6 0.4216444132115249 1.7999999999999998 7 0.3865073787772312 1.925 8 0.21082220660576245 1.2 9 0.2459592410400562 1.575 >10 0.7378777231201686 9.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 38 0.95 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 38 0.95 Illumina Single End PCR Primer 1 (100% over 50bp) CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 30 0.75 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 26 0.65 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 23 0.575 No Hit GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 20 0.5 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 19 0.475 No Hit GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG 18 0.44999999999999996 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 17 0.42500000000000004 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 17 0.42500000000000004 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 15 0.375 No Hit GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC 15 0.375 No Hit GGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGA 13 0.325 No Hit GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA 12 0.3 No Hit GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT 11 0.27499999999999997 No Hit AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG 11 0.27499999999999997 No Hit GGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC 11 0.27499999999999997 No Hit CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC 10 0.25 No Hit CCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT 10 0.25 No Hit CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT 10 0.25 No Hit CCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT 10 0.25 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 9 0.22499999999999998 No Hit GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA 9 0.22499999999999998 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 9 0.22499999999999998 No Hit CCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGG 9 0.22499999999999998 No Hit AAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA 9 0.22499999999999998 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 9 0.22499999999999998 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 9 0.22499999999999998 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 8 0.2 No Hit AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGG 8 0.2 No Hit CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG 8 0.2 No Hit TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA 8 0.2 No Hit CGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAG 8 0.2 No Hit GGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAG 8 0.2 No Hit GCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA 7 0.17500000000000002 No Hit GCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAAC 7 0.17500000000000002 No Hit GTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGG 7 0.17500000000000002 No Hit GTAACATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATA 7 0.17500000000000002 No Hit GGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATG 7 0.17500000000000002 No Hit CTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGT 7 0.17500000000000002 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC 7 0.17500000000000002 No Hit AGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGC 7 0.17500000000000002 No Hit GGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT 7 0.17500000000000002 No Hit GAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAG 7 0.17500000000000002 No Hit GGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGT 7 0.17500000000000002 No Hit TCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAA 6 0.15 No Hit GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA 6 0.15 No Hit GTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAGGTTA 6 0.15 No Hit CGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGT 6 0.15 No Hit GGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATA 6 0.15 No Hit CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT 6 0.15 No Hit GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGC 6 0.15 No Hit CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA 6 0.15 No Hit CGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGA 6 0.15 No Hit CGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAG 6 0.15 No Hit GGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTAT 6 0.15 No Hit CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG 6 0.15 No Hit GTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTT 5 0.125 No Hit AGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTG 5 0.125 No Hit GTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGAAGTAGGTCC 5 0.125 No Hit GTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG 5 0.125 No Hit AAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCT 5 0.125 No Hit ACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTT 5 0.125 No Hit CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC 5 0.125 No Hit GCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGT 5 0.125 No Hit ATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGAATACCAAG 5 0.125 No Hit CGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGC 5 0.125 No Hit TACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGC 5 0.125 No Hit TGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTC 5 0.125 No Hit ACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG 5 0.125 No Hit AAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGG 5 0.125 No Hit GCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTCGTGAATTCATCTCC 5 0.125 No Hit GTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTC 5 0.125 No Hit GTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTAT 5 0.125 No Hit CTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.11249999999999999 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.4625 0.0 0.0 0.0 0.0 84-85 0.6875 0.0 0.0 0.0 0.0 86-87 0.9125 0.0 0.0 0.0 0.0 88-89 1.15 0.0 0.0 0.0 0.0 90-91 1.425 0.0 0.0 0.0 0.0 92-93 1.725 0.0 0.0 0.0 0.0 94-95 1.9625000000000001 0.0 0.0 0.0 0.0 96-97 2.3625 0.0 0.0 0.0 0.0 98-99 2.9625 0.0 0.0 0.0 0.0 100-101 3.3875 0.0 0.0 0.0 0.0 102-103 3.8 0.0 0.0 0.0 0.0 104-105 4.425 0.0 0.0 0.0 0.0 106-107 5.025 0.0 0.0 0.0 0.0 108-109 5.6125 0.0 0.0 0.0 0.0 110-111 6.262499999999999 0.0 0.0 0.0 0.0 112-113 6.975 0.0 0.0 0.0 0.0 114-115 7.675000000000001 0.0 0.0 0.0 0.0 116-117 8.45 0.0 0.0 0.0 0.0 118-119 9.1625 0.0 0.0 0.0 0.0 120-121 9.8125 0.0 0.0 0.0 0.0 122-123 10.4875 0.0 0.0 0.0 0.0 124-125 11.3375 0.0 0.0 0.0 0.0 126-127 12.1375 0.0 0.0 0.0 0.0 128-129 13.1375 0.0 0.0 0.0 0.0 130-131 13.9625 0.0 0.0 0.0 0.0 132-133 15.0 0.0 0.0 0.0 0.0 134-135 15.825 0.0 0.0 0.0 0.0 136-137 16.8125 0.0 0.0 0.0 0.0 138-139 17.8625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGCCT 10 0.006830828 145.0 1 AAAAAAA 215 0.007287582 6.7441864 60-64 >>END_MODULE Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716754 spots for SRR7473364.sra Written 716754 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra Read 716738 spots for SRR7473364.sra Written 716738 spots for SRR7473364.sra SRR ids: ['SRR7473364.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7k2ina2u SRR7473364.sra spots: 14334776 blocks: [[1, 716738], [716739, 1433476], [1433477, 2150214], [2150215, 2866952], [2866953, 3583690], [3583691, 4300428], [4300429, 5017166], [5017167, 5733904], [5733905, 6450642], [6450643, 7167380], [7167381, 7884118], [7884119, 8600856], [8600857, 9317594], [9317595, 10034332], [10034333, 10751070], [10751071, 11467808], [11467809, 12184546], [12184547, 12901284], [12901285, 13618022], [13618023, 14334776]] SRR7473364 file size 4835884 SRR7473364 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473364 SRR7473364_1.fastq SRR7473364_2.fastq Input file: SRR7473364_1.fastq Paired file: SRR7473364_2.fastq trimmed: SRR7473364-trimmed-pair1.fastq, SRR7473364-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 15:15:36 2024 >> started Sat Dec 7 15:15:53 2024 >> done (17.038s) 14334776 read pairs processed; of these: 49805 ( 0.35%) short read pairs filtered out after trimming by size control 143686 ( 1.00%) empty read pairs filtered out after trimming by size control 14141285 (98.65%) read pairs available; of these: 7908667 (55.93%) trimmed read pairs available after processing 6232618 (44.07%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 10 0.00% 20 6 0.00% 21 15 0.00% 22 6 0.00% 23 20 0.00% 24 31 0.00% 25 22 0.00% 26 13 0.00% 27 24 0.00% 28 25 0.00% 29 39 0.00% 30 43 0.00% 31 46 0.00% 32 78 0.00% 33 59 0.00% 34 43 0.00% 35 75 0.00% 36 67 0.00% 37 99 0.00% 38 103 0.00% 39 96 0.00% 40 109 0.00% 41 137 0.00% 42 152 0.00% 43 157 0.00% 44 184 0.00% 45 215 0.00% 46 266 0.00% 47 267 0.00% 48 335 0.00% 49 348 0.00% 50 438 0.00% 51 426 0.00% 52 516 0.00% 53 471 0.00% 54 546 0.00% 55 547 0.00% 56 556 0.00% 57 648 0.00% 58 783 0.01% 59 879 0.01% 60 921 0.01% 61 1141 0.01% 62 1217 0.01% 63 1414 0.01% 64 1502 0.01% 65 1879 0.01% 66 2523 0.02% 67 4608 0.03% 68 9422 0.07% 69 38829 0.27% 70 72183 0.51% 71 33299 0.24% 72 15697 0.11% 73 11044 0.08% 74 8996 0.06% 75 7820 0.06% 76 6927 0.05% 77 7083 0.05% 78 7273 0.05% 79 8376 0.06% 80 8664 0.06% 81 9036 0.06% 82 10451 0.07% 83 12762 0.09% 84 16978 0.12% 85 17865 0.13% 86 19372 0.14% 87 20333 0.14% 88 24440 0.17% 89 24013 0.17% 90 25624 0.18% 91 27139 0.19% 92 25785 0.18% 93 32532 0.23% 94 32897 0.23% 95 37695 0.27% 96 36362 0.26% 97 36470 0.26% 98 35806 0.25% 99 37254 0.26% 100 42466 0.30% 101 37168 0.26% 102 39837 0.28% 103 40809 0.29% 104 43943 0.31% 105 51558 0.36% 106 46637 0.33% 107 45039 0.32% 108 48975 0.35% 109 60222 0.43% 110 62590 0.44% 111 51281 0.36% 112 52094 0.37% 113 65377 0.46% 114 58108 0.41% 115 63602 0.45% 116 66496 0.47% 117 59256 0.42% 118 61995 0.44% 119 61039 0.43% 120 65968 0.47% 121 59452 0.42% 122 66491 0.47% 123 70159 0.50% 124 69121 0.49% 125 68048 0.48% 126 67771 0.48% 127 70225 0.50% 128 70025 0.50% 129 70678 0.50% 130 74626 0.53% 131 74435 0.53% 132 75413 0.53% 133 80010 0.57% 134 87680 0.62% 135 89256 0.63% 136 87579 0.62% 137 98055 0.69% 138 99034 0.70% 139 98627 0.70% 140 97586 0.69% 141 110407 0.78% 142 107289 0.76% 143 114196 0.81% 144 123261 0.87% 145 137365 0.97% 146 153164 1.08% 147 186577 1.32% 148 263515 1.86% 149 498242 3.52% 150 2773376 19.61% 151 6232618 44.07% 14141285 reads passed initial QC criterion=sequence-density sequence-density=1.14 sequence-density-rank=1 fanout-score=1.70 fanout-score-rank=38 prefix-density=1.94 prefix-fanout=1.0 sequence=TAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG criterion=fanout-score sequence-density=0.22 sequence-density-rank=33 fanout-score=11.62 fanout-score-rank=1 prefix-density=2.50 prefix-fanout=1.0 sequence=CCATCAGGCAGCTTCCCAGACAT criterion=sequence-density sequence-density=5.34 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=38 prefix-density=5.34 prefix-fanout=1.9 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=14.80 fanout-score-rank=1 prefix-density=0.03 prefix-fanout=2.8 sequence=CACCAGAGCCAGCCTCACACTCTTAGGAGAGCACGGTACAGCAGTACATCAATGGCGACCGCCACCATGGCCCTCTCCTCCTCGACCTTCGCCGGGAAGGCGGTGAAGAACCTGCCGGCGCTCGGAGAGGCCCGCATCACCATGCGCAAGACCGTTGCCAAGGCCAAGCCGGTCTCCTCAGGCAGCCCGTG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG -y GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG -o SRR7473364 SRR7473364_1.fastq SRR7473364_2.fastq Input file: SRR7473364_1.fastq Paired file: SRR7473364_2.fastq trimmed: SRR7473364-trimmed-pair1.fastq, SRR7473364-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCAT -- paired 3' end adapter sequence (-y): GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACT -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 15:17:32 2024 >> started Sat Dec 7 15:17:45 2024 >> done (13.355s) 7070643 read pairs processed; of these: 124 ( 0.00%) short read pairs filtered out after trimming by size control 1049 ( 0.01%) empty read pairs filtered out after trimming by size control 7069470 (99.98%) read pairs available; of these: 2018 ( 0.03%) trimmed read pairs available after processing 7067452 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 7 0.00% 20 6 0.00% 21 9 0.00% 22 5 0.00% 23 16 0.00% 24 17 0.00% 25 13 0.00% 26 14 0.00% 27 18 0.00% 28 27 0.00% 29 25 0.00% 30 34 0.00% 31 28 0.00% 32 46 0.00% 33 38 0.00% 34 21 0.00% 35 34 0.00% 36 41 0.00% 37 40 0.00% 38 56 0.00% 39 50 0.00% 40 60 0.00% 41 77 0.00% 42 82 0.00% 43 73 0.00% 44 99 0.00% 45 98 0.00% 46 148 0.00% 47 132 0.00% 48 176 0.00% 49 167 0.00% 50 217 0.00% 51 225 0.00% 52 255 0.00% 53 239 0.00% 54 266 0.00% 55 284 0.00% 56 269 0.00% 57 322 0.00% 58 371 0.01% 59 430 0.01% 60 448 0.01% 61 591 0.01% 62 580 0.01% 63 724 0.01% 64 759 0.01% 65 967 0.01% 66 1269 0.02% 67 2335 0.03% 68 4658 0.07% 69 19466 0.28% 70 36104 0.51% 71 16642 0.24% 72 7906 0.11% 73 5571 0.08% 74 4575 0.06% 75 3840 0.05% 76 3413 0.05% 77 3555 0.05% 78 3688 0.05% 79 4174 0.06% 80 4235 0.06% 81 4516 0.06% 82 5228 0.07% 83 6409 0.09% 84 8428 0.12% 85 9021 0.13% 86 9802 0.14% 87 10120 0.14% 88 12228 0.17% 89 11888 0.17% 90 12877 0.18% 91 13558 0.19% 92 12748 0.18% 93 16393 0.23% 94 16347 0.23% 95 18853 0.27% 96 18175 0.26% 97 18161 0.26% 98 17815 0.25% 99 18540 0.26% 100 21359 0.30% 101 18629 0.26% 102 20043 0.28% 103 20197 0.29% 104 21972 0.31% 105 25806 0.37% 106 23403 0.33% 107 22599 0.32% 108 24462 0.35% 109 30109 0.43% 110 31105 0.44% 111 25602 0.36% 112 26094 0.37% 113 32771 0.46% 114 29163 0.41% 115 31842 0.45% 116 33353 0.47% 117 29547 0.42% 118 31001 0.44% 119 30456 0.43% 120 32992 0.47% 121 29825 0.42% 122 33338 0.47% 123 35134 0.50% 124 34654 0.49% 125 34168 0.48% 126 33718 0.48% 127 35148 0.50% 128 35017 0.50% 129 35219 0.50% 130 37382 0.53% 131 37112 0.52% 132 37641 0.53% 133 40178 0.57% 134 43561 0.62% 135 44703 0.63% 136 43588 0.62% 137 48958 0.69% 138 49606 0.70% 139 49390 0.70% 140 48690 0.69% 141 55327 0.78% 142 53956 0.76% 143 57005 0.81% 144 61490 0.87% 145 68961 0.98% 146 76472 1.08% 147 93110 1.32% 148 131863 1.87% 149 249321 3.53% 150 1385600 19.60% 151 3115682 44.07% criterion=sequence-density sequence-density=1.15 sequence-density-rank=1 fanout-score=1.71 fanout-score-rank=37 prefix-density=1.96 prefix-fanout=1.0 sequence=TAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG criterion=fanout-score sequence-density=0.22 sequence-density-rank=32 fanout-score=11.66 fanout-score-rank=1 prefix-density=2.49 prefix-fanout=1.0 sequence=CCATCAGGCAGCTTCCCAGACAT criterion=sequence-density sequence-density=5.29 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=39 prefix-density=5.30 prefix-fanout=1.9 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=14.91 fanout-score-rank=1 prefix-density=0.03 prefix-fanout=2.8 sequence=CACCAGAGCCAGCCTCACACTCTTAGGAGAGCACGGTACAGCAGTACATCAATGGCGACCGCCACCATGGCCCTCTCCTCCTCGACCTTCGCCGGGAAGGCGGTGAAGAACCTGCCGGCGCTCGGAGAGGCCCGCATCACCATGCGCAAGACCGTTGCCAAGGCCAAGCCGGTCTCCTCAGGCAGCCCGTG SRR7473364 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 15:19:47 Started mapping on | Dec 07 15:19:47 Finished on | Dec 07 15:40:14 Mapping speed, Million of reads per hour | 41.49 Number of input reads | 14140112 Average input read length | 282 UNIQUE READS: Uniquely mapped reads number | 5360040 Uniquely mapped reads % | 37.91% Average mapped length | 288.61 Number of splices: Total | 3728590 Number of splices: Annotated (sjdb) | 3497321 Number of splices: GT/AG | 3681049 Number of splices: GC/AG | 40874 Number of splices: AT/AC | 1326 Number of splices: Non-canonical | 5341 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.00% Deletion average length | 1.45 Insertion rate per base | 0.01% Insertion average length | 1.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 76503 % of reads mapped to multiple loci | 0.54% Number of reads mapped to too many loci | 5036 % of reads mapped to too many loci | 0.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 58.04% % of reads unmapped: other | 3.48% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8715658 8715658 8715658 N_multimapping 76503 76503 76503 N_noFeature 144003 5153618 198546 N_ambiguous 183101 603 31676 UnstrandedReadsAssigned:5032936 PositiveStrandReadsAssigned:205819 NegativeStrandReadsAssigned:5129818 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=135 echo kmer=131 SRR7473364 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473364-trimmed-pair1.fastq SRR7473364-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,140,112 reads, 5,309,416 reads pseudoaligned [quant] estimated average fragment length: 220.802 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,037 rounds 52973 SRR7473364.ke.tsv 35125 SRR7473364.se.tsv 88098 total ==> SRR7473364.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 716.373 0 0 PNS24247 1044 824.198 0 0 PNS24249 1928 1708.2 0 0 PNS24246 1044 824.198 0 0 PNS24248 1044 824.198 0 0 PNS24244 1471 1251.2 68 11.3833 PNS24243 293 108.683 0 0 KQK14069 1603 1383.2 405.608 61.4194 KQK14071 474 263.331 4.8999 3.89735 ==> SRR7473364.se.tsv <== BRADI_1g14170v3 419 BRADI_1g53295v3 1 BRADI_1g59795v3 167 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 326 BRADI_1g74790v3 60 BRADI_1g09890v3 5 BRADI_1g77505v3 130 BRADI_1g48960v3 0 SRR7473364 completed mapping pipeline successfully