Starting /dee2/code/volunteer_pipeline.sh SRR7473367 current disk space = 1542508851200 free memory = 1600654460 SRR7473367 SRAfilesize b17275bd6e169e16efb706a80a0ef5dc SRR7473367.sra SRR7473367.sra file validated SRR7473367 is paired end SRR7473367 is conventional basespace SRR7473367 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473367_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.536 34.0 34.0 34.0 33.0 34.0 2 33.546 34.0 34.0 34.0 33.0 34.0 3 33.59675 34.0 34.0 34.0 33.0 34.0 4 33.52775 34.0 34.0 34.0 33.0 34.0 5 33.59 34.0 34.0 34.0 33.0 34.0 6 37.357 38.0 38.0 38.0 37.0 38.0 7 37.56025 38.0 38.0 38.0 38.0 38.0 8 37.66875 38.0 38.0 38.0 38.0 38.0 9 37.647 38.0 38.0 38.0 38.0 38.0 10-14 37.586149999999996 38.0 38.0 38.0 38.0 38.0 15-19 37.643350000000005 38.0 38.0 38.0 38.0 38.0 20-24 37.60525 38.0 38.0 38.0 38.0 38.0 25-29 37.5395 38.0 38.0 38.0 38.0 38.0 30-34 37.469649999999994 38.0 38.0 38.0 37.8 38.0 35-39 37.2915 38.0 38.0 38.0 37.2 38.0 40-44 37.30845000000001 38.0 38.0 38.0 37.4 38.0 45-49 37.299499999999995 38.0 38.0 38.0 37.0 38.0 50-54 37.29055 38.0 38.0 38.0 37.0 38.0 55-59 37.121 38.0 38.0 38.0 36.6 38.0 60-64 37.030950000000004 38.0 38.0 38.0 36.2 38.0 65-69 36.82505 38.0 38.0 38.0 35.2 38.0 70-74 37.098200000000006 38.0 38.0 38.0 36.6 38.0 75-79 36.71155 38.0 38.0 38.0 36.0 38.0 80-84 36.6888 38.0 38.0 38.0 36.0 38.0 85-89 36.6186 38.0 38.0 38.0 36.0 38.0 90-94 36.45425 38.0 38.0 38.0 35.2 38.0 95-99 36.4432 38.0 38.0 38.0 35.0 38.0 100-104 36.31165 38.0 38.0 38.0 34.4 38.0 105-109 36.2219 38.0 38.0 38.0 34.2 38.0 110-114 36.09865 38.0 38.0 38.0 34.0 38.0 115-119 35.960449999999994 38.0 38.0 38.0 33.6 38.0 120-124 35.79735000000001 38.0 38.0 38.0 33.0 38.0 125-129 35.376999999999995 38.0 36.4 38.0 31.0 38.0 130-134 35.0241 38.0 36.0 38.0 29.8 38.0 135-139 34.484700000000004 38.0 35.2 38.0 26.2 38.0 140-144 34.30910000000001 38.0 34.8 38.0 26.4 38.0 145-149 33.69705 38.0 33.4 38.0 23.2 38.0 150-151 29.089125000000003 35.5 24.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 1.0 10 1.0 11 1.0 12 1.0 13 3.0 14 8.0 15 7.0 16 5.0 17 3.0 18 13.0 19 24.0 20 3.0 21 4.0 22 2.0 23 7.0 24 10.0 25 11.0 26 15.0 27 15.0 28 27.0 29 23.0 30 36.0 31 39.0 32 51.0 33 70.0 34 115.0 35 209.0 36 555.0 37 2739.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.925 13.525 12.675 32.875 2 24.137068534267133 16.983491745872936 32.46623311655828 26.413206603301653 3 21.175 23.075000000000003 27.474999999999998 28.275 4 22.8 28.375 23.474999999999998 25.35 5 25.55 31.35 24.275 18.825 6 21.7 30.025000000000002 26.55 21.725 7 14.95 22.375 41.675000000000004 21.0 8 19.125 22.650000000000002 29.799999999999997 28.425 9 19.075 21.3 32.074999999999996 27.55 10-14 21.68 25.535000000000004 25.825 26.96 15-19 21.78 25.474999999999998 26.815 25.929999999999996 20-24 21.335 26.584999999999997 26.775 25.305 25-29 20.66 25.974999999999998 27.339999999999996 26.025 30-34 20.905 25.080000000000002 26.515 27.500000000000004 35-39 21.54 25.8 27.284999999999997 25.374999999999996 40-44 21.77 25.759999999999998 26.669999999999998 25.8 45-49 22.650000000000002 26.125 26.045 25.180000000000003 50-54 22.485 25.455 25.845000000000002 26.215 55-59 21.279999999999998 25.05 27.54 26.13 60-64 21.224999999999998 25.130000000000003 27.16 26.484999999999996 65-69 21.265 25.96 26.76 26.015 70-74 21.775 26.465 25.72 26.040000000000003 75-79 21.57 26.584999999999997 25.305 26.540000000000003 80-84 22.205 26.545 25.945 25.305 85-89 21.905 26.145000000000003 25.91 26.040000000000003 90-94 21.695 25.965 26.21 26.13 95-99 21.88 26.224999999999998 25.995 25.900000000000002 100-104 22.33 26.745 25.009999999999998 25.915 105-109 21.535 26.005 26.405 26.055 110-114 21.665 26.015 25.715 26.605 115-119 21.45 27.029999999999998 25.480000000000004 26.040000000000003 120-124 22.295 26.640000000000004 24.825 26.240000000000002 125-129 22.470000000000002 26.02 26.045 25.465 130-134 22.444077465845968 26.18725917029475 25.56673172196367 25.80193164189561 135-139 21.95268343920372 26.29920472165258 26.014104936727854 25.734006902415846 140-144 22.86 26.145000000000003 25.095 25.900000000000002 145-149 22.2 26.534999999999997 25.97 25.295 150-151 22.3625 26.5875 24.7375 26.3125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 0.5 3 1.0 4 1.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 0.5 23 0.0 24 1.5 25 3.5 26 5.0 27 8.0 28 9.0 29 9.5 30 16.5 31 26.5 32 34.0 33 46.5 34 51.5 35 68.0 36 95.5 37 112.0 38 128.5 39 120.5 40 103.0 41 113.0 42 126.5 43 129.0 44 142.0 45 144.5 46 142.0 47 138.0 48 143.0 49 147.0 50 153.0 51 163.0 52 164.5 53 198.0 54 222.0 55 206.5 56 157.0 57 121.5 58 112.5 59 92.0 60 77.5 61 67.0 62 43.5 63 27.5 64 22.0 65 14.0 66 11.5 67 13.5 68 14.0 69 13.0 70 10.5 71 7.0 72 4.5 73 3.0 74 2.5 75 2.5 76 2.5 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.08499999999999999 135-139 0.034999999999999996 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.27499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 88.54451534487997 72.85000000000001 2 7.505317532664843 12.35 3 1.7016104527499238 4.2 4 1.0331206320267396 3.4000000000000004 5 0.6381039197812215 2.625 6 0.18231540565177756 0.8999999999999999 7 0.030385900941962928 0.17500000000000002 8 0.12154360376785171 0.8 9 0.15192950470981464 1.125 >10 0.09115770282588878 1.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC 33 0.8250000000000001 TruSeq Adapter, Index 9 (97% over 36bp) GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 18 0.44999999999999996 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCC 12 0.3 TruSeq Adapter, Index 9 (97% over 35bp) CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 9 0.22499999999999998 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 9 0.22499999999999998 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 9 0.22499999999999998 No Hit AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA 9 0.22499999999999998 No Hit CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 9 0.22499999999999998 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 8 0.2 No Hit GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC 8 0.2 No Hit GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG 8 0.2 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 8 0.2 No Hit GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG 7 0.17500000000000002 No Hit CGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGAT 6 0.15 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6 0.15 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 6 0.15 No Hit ATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG 6 0.15 No Hit CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 6 0.15 No Hit GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGA 6 0.15 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG 5 0.125 No Hit GTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTA 5 0.125 No Hit GCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGA 5 0.125 No Hit CAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCGC 5 0.125 No Hit CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG 5 0.125 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 5 0.125 No Hit GTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATT 5 0.125 No Hit GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT 5 0.125 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 5 0.125 No Hit ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC 5 0.125 No Hit GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG 5 0.125 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 5 0.125 No Hit CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAG 5 0.125 No Hit GCTCTACCCCCGGAGATGAATTCACGAGGCGCTACCTAAATAGCTTTCGG 5 0.125 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 5 0.125 No Hit CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC 5 0.125 No Hit CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC 5 0.125 No Hit CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT 5 0.125 No Hit CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT 5 0.125 No Hit CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCA 5 0.125 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.0875 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.2375 0.0 0.0 0.0 0.0 76-77 0.30000000000000004 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.55 0.0 0.0 0.0 0.0 84-85 0.7250000000000001 0.0 0.0 0.0 0.0 86-87 0.925 0.0 0.0 0.0 0.0 88-89 1.075 0.0 0.0 0.0 0.0 90-91 1.2125 0.0 0.0 0.0 0.0 92-93 1.3875 0.0 0.0 0.0 0.0 94-95 1.65 0.0 0.0 0.0 0.0 96-97 1.9375 0.0 0.0 0.0 0.0 98-99 2.1375 0.0 0.0 0.0 0.0 100-101 2.3875 0.0 0.0 0.0 0.0 102-103 2.7125 0.0 0.0 0.0 0.0 104-105 2.9749999999999996 0.0 0.0 0.0 0.0 106-107 3.6125 0.0 0.0 0.0 0.0 108-109 4.1 0.0 0.0 0.0 0.0 110-111 4.725 0.0 0.0 0.0 0.0 112-113 5.3125 0.0 0.0 0.0 0.0 114-115 5.887499999999999 0.0 0.0 0.0 0.0 116-117 6.4375 0.0 0.0 0.0 0.0 118-119 7.0125 0.0 0.0 0.0 0.0 120-121 7.825 0.0 0.0 0.0 0.0 122-123 8.425 0.0 0.0 0.0 0.0 124-125 9.3625 0.0 0.0 0.0 0.0 126-127 10.1 0.0 0.0 0.0 0.0 128-129 10.8125 0.0 0.0 0.0 0.0 130-131 11.35 0.0 0.0 0.0 0.0 132-133 12.05 0.0 0.0 0.0 0.0 134-135 12.7375 0.0 0.0 0.0 0.0 136-137 13.5125 0.0 0.0 0.0 0.0 138-139 14.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR7473367 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473367_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.991 34.0 33.0 34.0 32.0 34.0 2 33.0325 34.0 33.0 34.0 33.0 34.0 3 32.9285 34.0 33.0 34.0 33.0 34.0 4 32.80625 34.0 33.0 34.0 32.0 34.0 5 32.9185 34.0 33.0 34.0 33.0 34.0 6 36.41175 38.0 38.0 38.0 35.0 38.0 7 36.8705 38.0 38.0 38.0 37.0 38.0 8 36.75275 38.0 38.0 38.0 37.0 38.0 9 36.90425 38.0 38.0 38.0 37.0 38.0 10-14 36.8486 38.0 38.0 38.0 37.0 38.0 15-19 36.79715 38.0 38.0 38.0 36.8 38.0 20-24 36.75165 38.0 38.0 38.0 36.6 38.0 25-29 36.86325 38.0 38.0 38.0 37.2 38.0 30-34 36.8789 38.0 38.0 38.0 37.2 38.0 35-39 36.813100000000006 38.0 38.0 38.0 37.4 38.0 40-44 36.81765 38.0 38.0 38.0 37.0 38.0 45-49 36.68755 38.0 38.0 38.0 36.6 38.0 50-54 36.8192 38.0 38.0 38.0 37.0 38.0 55-59 36.817 38.0 38.0 38.0 37.0 38.0 60-64 36.767399999999995 38.0 38.0 38.0 37.0 38.0 65-69 36.63875 38.0 38.0 38.0 36.6 38.0 70-74 36.3427 38.0 38.0 38.0 36.2 38.0 75-79 36.2799 38.0 38.0 38.0 36.0 38.0 80-84 36.2664 38.0 38.0 38.0 36.0 38.0 85-89 36.13315 38.0 38.0 38.0 35.2 38.0 90-94 35.9896 38.0 38.0 38.0 34.6 38.0 95-99 35.95385 38.0 38.0 38.0 34.0 38.0 100-104 35.839800000000004 38.0 38.0 38.0 34.0 38.0 105-109 35.69984999999999 38.0 38.0 38.0 33.8 38.0 110-114 35.2566 38.0 37.8 38.0 31.4 38.0 115-119 35.28960000000001 38.0 38.0 38.0 31.8 38.0 120-124 34.757549999999995 38.0 36.6 38.0 27.0 38.0 125-129 34.6444 38.0 36.2 38.0 28.0 38.0 130-134 34.3516 38.0 36.0 38.0 26.6 38.0 135-139 34.054500000000004 38.0 35.4 38.0 24.4 38.0 140-144 33.419599999999996 38.0 34.4 38.0 19.2 38.0 145-149 32.43344999999999 38.0 33.0 38.0 10.2 38.0 150-151 27.144875 33.0 17.5 37.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24.0 3 8.0 4 10.0 5 4.0 6 0.0 7 1.0 8 2.0 9 4.0 10 6.0 11 10.0 12 7.0 13 5.0 14 4.0 15 8.0 16 9.0 17 31.0 18 9.0 19 9.0 20 3.0 21 8.0 22 7.0 23 4.0 24 9.0 25 11.0 26 23.0 27 14.0 28 24.0 29 20.0 30 20.0 31 42.0 32 63.0 33 72.0 34 122.0 35 187.0 36 509.0 37 2711.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.55 20.0 12.45 25.0 2 31.36568284142071 22.0360180090045 26.463231615807903 20.135067533766886 3 24.7935951963973 27.395546659994995 26.044533400050035 21.766324743557668 4 26.91345672836418 32.016008004002 19.734867433716857 21.335667833916958 5 28.68934467233617 33.291645822911455 19.209604802401202 18.809404702351177 6 24.825 35.3 19.75 20.125 7 23.799999999999997 20.349999999999998 33.324999999999996 22.525000000000002 8 26.55 24.4 22.375 26.674999999999997 9 25.45 23.974999999999998 24.775 25.8 10-14 26.845000000000002 25.765 22.715 24.675 15-19 27.965 25.745 22.905 23.385 20-24 28.345 26.355 22.475 22.825 25-29 26.064999999999998 27.08 23.905 22.95 30-34 27.66 25.840000000000003 24.349999999999998 22.15 35-39 26.815 25.779999999999998 24.5 22.905 40-44 28.075 25.490000000000002 24.585 21.85 45-49 27.375 25.729999999999997 24.65 22.245 50-54 26.169999999999998 25.430000000000003 26.005 22.395 55-59 26.0 26.705000000000002 25.790000000000003 21.505 60-64 25.8 26.674999999999997 25.145 22.38 65-69 26.525 27.060000000000002 25.14 21.275 70-74 26.179999999999996 26.99 25.130000000000003 21.7 75-79 25.885 27.045 24.54 22.53 80-84 26.295 27.725 25.174999999999997 20.805 85-89 27.075 26.640000000000004 24.625 21.66 90-94 27.255000000000003 25.825 25.44 21.48 95-99 26.615 27.155 25.155 21.075 100-104 27.155 27.665 24.560000000000002 20.62 105-109 26.295 28.065 23.765 21.875 110-114 27.092191486168776 28.22270021509679 23.345505477464858 21.339602821269573 115-119 26.471912360562257 28.68290730828873 24.29093091891351 20.554249412235507 120-124 26.86768676867687 27.702770277027707 24.202420242024203 21.227122712271225 125-129 26.735 28.299999999999997 24.295 20.669999999999998 130-134 27.37 26.979999999999997 24.525 21.125 135-139 27.644999999999996 27.395000000000003 23.935000000000002 21.025 140-144 27.32 27.169999999999998 24.815 20.695 145-149 28.439999999999998 28.13 23.195 20.235 150-151 28.349999999999998 27.700000000000003 23.925 20.025000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.5 15 0.5 16 0.5 17 0.5 18 0.0 19 0.0 20 1.0 21 2.5 22 2.5 23 1.5 24 2.0 25 2.0 26 3.0 27 6.5 28 9.0 29 11.0 30 17.5 31 24.0 32 30.0 33 34.5 34 44.5 35 53.0 36 67.5 37 76.0 38 85.0 39 96.0 40 103.5 41 110.0 42 109.5 43 112.0 44 112.5 45 110.0 46 110.0 47 129.5 48 149.5 49 171.5 50 186.0 51 172.5 52 178.5 53 219.5 54 234.0 55 230.5 56 193.5 57 141.5 58 113.0 59 96.0 60 80.0 61 73.0 62 69.5 63 43.5 64 21.0 65 16.5 66 22.5 67 21.0 68 19.0 69 18.0 70 13.5 71 9.5 72 9.0 73 8.0 74 6.5 75 5.0 76 2.5 77 2.0 78 1.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.075 4 0.05 5 0.05 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.045 115-119 0.045 120-124 0.01 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.35 #Duplication Level Percentage of deduplicated Percentage of total 1 88.67638129933212 73.02499999999999 2 7.498482088646023 12.35 3 1.7911353976927749 4.425 4 0.8803885853066181 2.9000000000000004 5 0.546448087431694 2.25 6 0.21250758955676988 1.05 7 0.12143290831815423 0.7000000000000001 8 0.060716454159077116 0.4 9 0.030358227079538558 0.22499999999999998 >10 0.18214936247723132 2.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 33 0.8250000000000001 Illumina Single End PCR Primer 1 (100% over 50bp) GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 20 0.5 No Hit CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 17 0.42500000000000004 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 15 0.375 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 12 0.3 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 10 0.25 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 9 0.22499999999999998 No Hit CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC 8 0.2 No Hit GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 8 0.2 No Hit GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA 7 0.17500000000000002 No Hit GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG 7 0.17500000000000002 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 7 0.17500000000000002 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC 7 0.17500000000000002 No Hit GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG 6 0.15 No Hit TAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATG 6 0.15 No Hit GTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGAT 6 0.15 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 6 0.15 No Hit GTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGC 6 0.15 No Hit GGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGA 6 0.15 No Hit GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC 6 0.15 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 5 0.125 No Hit GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 5 0.125 No Hit AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGG 5 0.125 No Hit AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT 5 0.125 No Hit GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT 5 0.125 No Hit CCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT 5 0.125 No Hit GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTG 5 0.125 No Hit AAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA 5 0.125 No Hit GGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT 5 0.125 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 5 0.125 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 5 0.125 No Hit GGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGT 5 0.125 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 5 0.125 No Hit CTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTTCAAC 5 0.125 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 5 0.125 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 5 0.125 No Hit GGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTAC 5 0.125 No Hit CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.16249999999999998 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3375 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.8 0.0 0.0 0.0 0.0 86-87 0.9625 0.0 0.0 0.0 0.0 88-89 1.1 0.0 0.0 0.0 0.0 90-91 1.2625000000000002 0.0 0.0 0.0 0.0 92-93 1.425 0.0 0.0 0.0 0.0 94-95 1.6749999999999998 0.0 0.0 0.0 0.0 96-97 1.9625 0.0 0.0 0.0 0.0 98-99 2.1625 0.0 0.0 0.0 0.0 100-101 2.4375 0.0 0.0 0.0 0.0 102-103 2.825 0.0 0.0 0.0 0.0 104-105 3.1125 0.0 0.0 0.0 0.0 106-107 3.7125000000000004 0.0 0.0 0.0 0.0 108-109 4.175 0.0 0.0 0.0 0.0 110-111 4.800000000000001 0.0 0.0 0.0 0.0 112-113 5.362500000000001 0.0 0.0 0.0 0.0 114-115 5.9125 0.0 0.0 0.0 0.0 116-117 6.4875 0.0 0.0 0.0 0.0 118-119 7.075 0.0 0.0 0.0 0.0 120-121 7.887499999999999 0.0 0.0 0.0 0.0 122-123 8.5 0.0 0.0 0.0 0.0 124-125 9.375 0.0 0.0 0.0 0.0 126-127 10.1375 0.0 0.0 0.0 0.0 128-129 10.8625 0.0 0.0 0.0 0.0 130-131 11.45 0.0 0.0 0.0 0.0 132-133 12.1875 0.0 0.0 0.0 0.0 134-135 12.925 0.0 0.0 0.0 0.0 136-137 13.6875 0.0 0.0 0.0 0.0 138-139 14.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAAAA 210 0.00580008 6.904762 60-64 >>END_MODULE Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra Read 752972 spots for SRR7473367.sra Written 752972 spots for SRR7473367.sra Read 752959 spots for SRR7473367.sra Written 752959 spots for SRR7473367.sra SRR ids: ['SRR7473367.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wg2zdd_v SRR7473367.sra spots: 15059193 blocks: [[1, 752959], [752960, 1505918], [1505919, 2258877], [2258878, 3011836], [3011837, 3764795], [3764796, 4517754], [4517755, 5270713], [5270714, 6023672], [6023673, 6776631], [6776632, 7529590], [7529591, 8282549], [8282550, 9035508], [9035509, 9788467], [9788468, 10541426], [10541427, 11294385], [11294386, 12047344], [12047345, 12800303], [12800304, 13553262], [13553263, 14306221], [14306222, 15059193]] SRR7473367 file size 5081366 SRR7473367 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473367 SRR7473367_1.fastq SRR7473367_2.fastq Input file: SRR7473367_1.fastq Paired file: SRR7473367_2.fastq trimmed: SRR7473367-trimmed-pair1.fastq, SRR7473367-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 15:19:38 2024 >> started Sat Dec 7 15:19:53 2024 >> done (15.510s) 15059193 read pairs processed; of these: 55899 ( 0.37%) short read pairs filtered out after trimming by size control 167749 ( 1.11%) empty read pairs filtered out after trimming by size control 14835545 (98.51%) read pairs available; of these: 8043686 (54.22%) trimmed read pairs available after processing 6791859 (45.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 10 0.00% 20 15 0.00% 21 21 0.00% 22 27 0.00% 23 31 0.00% 24 34 0.00% 25 27 0.00% 26 40 0.00% 27 46 0.00% 28 34 0.00% 29 48 0.00% 30 65 0.00% 31 64 0.00% 32 65 0.00% 33 75 0.00% 34 57 0.00% 35 80 0.00% 36 95 0.00% 37 83 0.00% 38 87 0.00% 39 107 0.00% 40 128 0.00% 41 155 0.00% 42 190 0.00% 43 142 0.00% 44 208 0.00% 45 235 0.00% 46 284 0.00% 47 314 0.00% 48 338 0.00% 49 336 0.00% 50 424 0.00% 51 458 0.00% 52 590 0.00% 53 527 0.00% 54 524 0.00% 55 558 0.00% 56 633 0.00% 57 665 0.00% 58 744 0.01% 59 842 0.01% 60 897 0.01% 61 1097 0.01% 62 1196 0.01% 63 1413 0.01% 64 1591 0.01% 65 2130 0.01% 66 2618 0.02% 67 4298 0.03% 68 7934 0.05% 69 30305 0.20% 70 37074 0.25% 71 12632 0.09% 72 7655 0.05% 73 6927 0.05% 74 6072 0.04% 75 5751 0.04% 76 5500 0.04% 77 5469 0.04% 78 5709 0.04% 79 6723 0.05% 80 7255 0.05% 81 8078 0.05% 82 9590 0.06% 83 11449 0.08% 84 15357 0.10% 85 16490 0.11% 86 17673 0.12% 87 18941 0.13% 88 20449 0.14% 89 20224 0.14% 90 21835 0.15% 91 23727 0.16% 92 23666 0.16% 93 28080 0.19% 94 28941 0.20% 95 31032 0.21% 96 30616 0.21% 97 29200 0.20% 98 28419 0.19% 99 29297 0.20% 100 32917 0.22% 101 31921 0.22% 102 34587 0.23% 103 37160 0.25% 104 40014 0.27% 105 45472 0.31% 106 42302 0.29% 107 40116 0.27% 108 42385 0.29% 109 48865 0.33% 110 49572 0.33% 111 44064 0.30% 112 47393 0.32% 113 58010 0.39% 114 53505 0.36% 115 58634 0.40% 116 59083 0.40% 117 54269 0.37% 118 54338 0.37% 119 52612 0.35% 120 56291 0.38% 121 53632 0.36% 122 58367 0.39% 123 63275 0.43% 124 66429 0.45% 125 67736 0.46% 126 65926 0.44% 127 65855 0.44% 128 64513 0.43% 129 65388 0.44% 130 66727 0.45% 131 67034 0.45% 132 69754 0.47% 133 74450 0.50% 134 79989 0.54% 135 85230 0.57% 136 86698 0.58% 137 92559 0.62% 138 93155 0.63% 139 90586 0.61% 140 91619 0.62% 141 100848 0.68% 142 102919 0.69% 143 111919 0.75% 144 124530 0.84% 145 142356 0.96% 146 167947 1.13% 147 208546 1.41% 148 300545 2.03% 149 584861 3.94% 150 3164056 21.33% 151 6791859 45.78% 14835545 reads passed initial QC criterion=sequence-density sequence-density=0.61 sequence-density-rank=1 fanout-score=6.61 fanout-score-rank=13 prefix-density=2.29 prefix-fanout=1.8 sequence=CACTCGTCAGCGAAACAGCAAGCTGTTTCCTGTTACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=62.18 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=7.3 sequence=AAAAAAAAGTATGTTTGCATCAATGATAATCCCAAAGTAGGAAGTGTGTACGTAGTAGCAAGTGTTATACATACATAAAATTAAGCGATGCGTTTCGATCAAGTACTTGGCATCATCGATCACATACGTACATTTCACAGCAGGTAGCTACGTACATTACAGGCATACGTACATGCAGAGAGATACCCGGCCCTGTGTTGTGTTTGCAATTGCATAGATGAGAATGAGATGACTTGATTCTTAATTAGCCGGCGAGAGCGCTGGTGTTGTAGACGAGGAGGGCGCCGCCGCCGAGGAGGCCGGCCAGGGTGACGGCCCAGATCAGGAGCCCCGTCGTCCCACCGGCGTAGCGGTCGCCAGATTCGGACCATACCTCCGGCGTGTAGATCGGGCTGTAGCCGTCGACGTTGGCGCCGTACTTGTCAACAAACTGGTACACACCCTTTCCCGTGCCCTTCCTGCCGTTGGCGTCGATGTCCACCGTCAAGCCACCTCCGATCCCGAGGGGCTT criterion=sequence-density sequence-density=2.93 sequence-density-rank=1 fanout-score=2.31 fanout-score-rank=30 prefix-density=3.12 prefix-fanout=2.2 sequence=GCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATG criterion=fanout-score sequence-density=0.18 sequence-density-rank=23 fanout-score=18.82 fanout-score-rank=1 prefix-density=2.97 prefix-fanout=1.1 sequence=AACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAGCGATTCAGCGCTATTTGCGCCAAGAACTGTTAACGTCTTGAATTCTGG SRR7473367 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 15:21:47 Started mapping on | Dec 07 15:21:47 Finished on | Dec 07 15:44:47 Mapping speed, Million of reads per hour | 38.70 Number of input reads | 14835545 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 6246223 Uniquely mapped reads % | 42.10% Average mapped length | 288.70 Number of splices: Total | 4717238 Number of splices: Annotated (sjdb) | 4356544 Number of splices: GT/AG | 4655160 Number of splices: GC/AG | 54208 Number of splices: AT/AC | 2636 Number of splices: Non-canonical | 5234 Mismatch rate per base, % | 0.12% Deletion rate per base | 0.01% Deletion average length | 1.41 Insertion rate per base | 0.01% Insertion average length | 1.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 113673 % of reads mapped to multiple loci | 0.77% Number of reads mapped to too many loci | 7186 % of reads mapped to too many loci | 0.05% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 55.05% % of reads unmapped: other | 2.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 8491529 8491529 8491529 N_multimapping 113673 113673 113673 N_noFeature 210794 6007021 275201 N_ambiguous 207884 1230 33875 UnstrandedReadsAssigned:5827545 PositiveStrandReadsAssigned:237972 NegativeStrandReadsAssigned:5937147 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=142 echo kmer=137 SRR7473367 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473367-trimmed-pair1.fastq SRR7473367-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,835,545 reads, 6,104,425 reads pseudoaligned [quant] estimated average fragment length: 225.992 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,132 rounds 52973 SRR7473367.ke.tsv 35125 SRR7473367.se.tsv 88098 total ==> SRR7473367.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 711.231 0 0 PNS24247 1044 819.008 0 0 PNS24249 1928 1703.01 6.78597 0.749085 PNS24246 1044 819.008 0 0 PNS24248 1044 819.008 0 0 PNS24244 1471 1246.01 83.214 12.5549 PNS24243 293 108.318 0 0 KQK14069 1603 1378.01 1156.45 157.765 KQK14071 474 261.033 39.3829 28.3628 ==> SRR7473367.se.tsv <== BRADI_1g14170v3 1288 BRADI_1g53295v3 18 BRADI_1g59795v3 157 BRADI_1g07683v3 0 BRADI_1g00485v3 17 BRADI_1g20270v3 345 BRADI_1g74790v3 22 BRADI_1g09890v3 2 BRADI_1g77505v3 320 BRADI_1g48960v3 0 SRR7473367 completed mapping pipeline successfully