Starting /dee2/code/volunteer_pipeline.sh SRR7473368 current disk space = 1542411333632 free memory = 1602767748 SRR7473368 SRAfilesize 93b2fdf50081a812ddd6ec486d235779 SRR7473368.sra SRR7473368.sra file validated SRR7473368 is paired end SRR7473368 is conventional basespace SRR7473368 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473368_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.54275 34.0 34.0 34.0 33.0 34.0 2 33.5555 34.0 34.0 34.0 33.0 34.0 3 33.57075 34.0 34.0 34.0 33.0 34.0 4 33.556 34.0 34.0 34.0 33.0 34.0 5 33.60125 34.0 34.0 34.0 33.0 34.0 6 37.382 38.0 38.0 38.0 37.0 38.0 7 37.56725 38.0 38.0 38.0 38.0 38.0 8 37.56775 38.0 38.0 38.0 38.0 38.0 9 37.6115 38.0 38.0 38.0 38.0 38.0 10-14 37.54825 38.0 38.0 38.0 37.8 38.0 15-19 37.600249999999996 38.0 38.0 38.0 38.0 38.0 20-24 37.57275 38.0 38.0 38.0 38.0 38.0 25-29 37.50985 38.0 38.0 38.0 37.8 38.0 30-34 37.3857 38.0 38.0 38.0 37.4 38.0 35-39 37.49095 38.0 38.0 38.0 38.0 38.0 40-44 37.160000000000004 38.0 38.0 38.0 36.8 38.0 45-49 37.312149999999995 38.0 38.0 38.0 37.2 38.0 50-54 37.211200000000005 38.0 38.0 38.0 37.2 38.0 55-59 37.13605 38.0 38.0 38.0 36.4 38.0 60-64 37.3102 38.0 38.0 38.0 37.2 38.0 65-69 37.070949999999996 38.0 38.0 38.0 36.6 38.0 70-74 37.19465 38.0 38.0 38.0 37.0 38.0 75-79 36.708749999999995 38.0 38.0 38.0 37.0 38.0 80-84 36.49185 38.0 38.0 38.0 35.8 38.0 85-89 36.61195 38.0 38.0 38.0 36.4 38.0 90-94 36.4862 38.0 38.0 38.0 36.0 38.0 95-99 36.4077 38.0 38.0 38.0 35.8 38.0 100-104 36.35435 38.0 38.0 38.0 35.0 38.0 105-109 36.169 38.0 38.0 38.0 34.4 38.0 110-114 36.10535 38.0 38.0 38.0 34.0 38.0 115-119 36.0539 38.0 38.0 38.0 34.0 38.0 120-124 35.8823 38.0 38.0 38.0 33.8 38.0 125-129 35.59975 38.0 38.0 38.0 33.0 38.0 130-134 35.433350000000004 38.0 37.6 38.0 32.6 38.0 135-139 35.0844 38.0 36.6 38.0 30.0 38.0 140-144 35.034549999999996 38.0 36.0 38.0 30.2 38.0 145-149 34.4486 38.0 35.8 38.0 27.0 38.0 150-151 31.14 36.5 31.0 38.0 11.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 1.0 10 1.0 11 0.0 12 2.0 13 3.0 14 3.0 15 6.0 16 8.0 17 8.0 18 22.0 19 27.0 20 6.0 21 6.0 22 4.0 23 5.0 24 6.0 25 13.0 26 12.0 27 12.0 28 20.0 29 23.0 30 31.0 31 26.0 32 41.0 33 60.0 34 88.0 35 164.0 36 416.0 37 2984.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.425 11.700000000000001 11.450000000000001 38.425 2 21.476846057571965 16.170212765957448 31.08886107634543 31.264080100125156 3 20.974999999999998 19.85 26.224999999999998 32.95 4 24.55 26.55 21.4 27.500000000000004 5 26.325 29.15 22.525000000000002 22.0 6 20.275000000000002 29.549999999999997 27.575 22.6 7 15.25 23.175 41.65 19.925 8 19.35 21.8 29.9 28.95 9 20.125 21.125 31.075000000000003 27.675 10-14 21.72 25.259999999999998 24.86 28.16 15-19 21.64 25.374999999999996 25.86 27.125 20-24 21.78 26.009999999999998 25.240000000000002 26.97 25-29 21.435000000000002 25.424999999999997 25.855 27.284999999999997 30-34 20.835 25.545 25.61 28.01 35-39 21.975 25.629999999999995 25.82 26.575 40-44 21.55 24.72 26.41 27.32 45-49 22.25 24.505 26.745 26.5 50-54 21.86 25.35 25.374999999999996 27.415 55-59 21.83 24.279999999999998 26.505000000000003 27.384999999999998 60-64 21.185000000000002 25.085 26.69 27.04 65-69 21.035 25.335 26.105 27.525 70-74 21.33 26.695 25.074999999999996 26.900000000000002 75-79 21.63 26.6 24.54 27.229999999999997 80-84 21.02 26.105 25.285000000000004 27.589999999999996 85-89 21.365000000000002 25.669999999999998 24.47 28.494999999999997 90-94 22.8 24.915000000000003 24.165 28.12 95-99 22.39 26.009999999999998 24.645 26.955000000000002 100-104 22.61 26.745 23.705000000000002 26.939999999999998 105-109 22.025 26.150000000000002 23.96 27.865000000000002 110-114 22.091104555227762 26.631331566578332 23.996199809990497 27.281364068203413 115-119 22.14 26.115 24.38 27.365000000000002 120-124 22.615 26.424999999999997 22.855 28.105000000000004 125-129 23.799999999999997 25.615 23.89 26.695 130-134 23.23 26.939999999999998 23.724999999999998 26.105 135-139 23.03 25.97 24.135 26.865 140-144 23.71 26.279999999999998 22.37 27.639999999999997 145-149 23.08192783143987 26.36004203993794 23.55737951053501 27.000650618087185 150-151 22.475 26.9125 23.0375 27.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.0 2 0.5 3 1.0 4 1.5 5 1.0 6 0.5 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 1.0 24 2.0 25 2.5 26 1.5 27 2.0 28 5.0 29 7.5 30 9.0 31 11.0 32 15.0 33 23.0 34 32.5 35 47.5 36 64.0 37 77.0 38 88.5 39 87.5 40 108.5 41 132.0 42 120.0 43 122.5 44 150.5 45 149.5 46 132.5 47 134.0 48 137.0 49 135.5 50 140.5 51 164.0 52 193.5 53 249.5 54 280.0 55 247.0 56 184.0 57 139.5 58 124.0 59 99.0 60 74.0 61 53.0 62 42.5 63 36.5 64 24.5 65 18.5 66 18.0 67 21.0 68 20.0 69 13.5 70 11.0 71 10.5 72 7.5 73 4.0 74 6.0 75 4.0 76 2.0 77 1.5 78 0.0 79 0.0 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.095 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.10000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 90.8138101109741 73.65 2 4.68557336621455 7.6 3 1.88039457459926 4.575 4 1.0480887792848335 3.4000000000000004 5 0.6165228113440198 2.5 6 0.21578298397040688 1.05 7 0.12330456226880394 0.7000000000000001 8 0.12330456226880394 0.8 9 0.09247842170160296 0.675 >10 0.4007398273736128 5.050000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACATCTCGTATGC 47 1.175 TruSeq Adapter, Index 7 (97% over 36bp) GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG 17 0.42500000000000004 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACATCTCGTATGCC 15 0.375 TruSeq Adapter, Index 7 (97% over 35bp) CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC 14 0.35000000000000003 No Hit CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA 14 0.35000000000000003 No Hit GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA 13 0.325 No Hit GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG 13 0.325 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 13 0.325 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG 13 0.325 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT 12 0.3 No Hit CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG 11 0.27499999999999997 No Hit GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGA 10 0.25 No Hit CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT 10 0.25 No Hit CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG 9 0.22499999999999998 No Hit GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG 9 0.22499999999999998 No Hit CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT 9 0.22499999999999998 No Hit CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG 8 0.2 No Hit GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC 8 0.2 No Hit CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG 8 0.2 No Hit CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG 8 0.2 No Hit GCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGC 7 0.17500000000000002 No Hit CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC 7 0.17500000000000002 No Hit CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT 7 0.17500000000000002 No Hit CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG 7 0.17500000000000002 No Hit GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC 6 0.15 No Hit GCCAGCTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCAC 6 0.15 No Hit GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACA 6 0.15 No Hit CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTA 6 0.15 No Hit CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT 6 0.15 No Hit CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC 6 0.15 No Hit CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC 6 0.15 No Hit CCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCC 5 0.125 No Hit CTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTC 5 0.125 No Hit CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT 5 0.125 No Hit GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTA 5 0.125 No Hit CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC 5 0.125 No Hit GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAG 5 0.125 No Hit GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA 5 0.125 No Hit GCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 5 0.125 No Hit GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA 5 0.125 No Hit CTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGC 5 0.125 No Hit GCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAA 5 0.125 No Hit CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA 5 0.125 No Hit CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC 5 0.125 No Hit CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA 5 0.125 No Hit CCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 5 0.125 No Hit GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG 5 0.125 No Hit CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT 5 0.125 No Hit GTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTC 5 0.125 No Hit GCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTT 5 0.125 No Hit AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.21250000000000002 0.0 0.0 0.0 0.0 72-73 0.25 0.0 0.0 0.0 0.0 74-75 0.3125 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.5 0.0 0.0 0.0 0.0 82-83 0.7375 0.0 0.0 0.0 0.0 84-85 1.0 0.0 0.0 0.0 0.0 86-87 1.275 0.0 0.0 0.0 0.0 88-89 1.4874999999999998 0.0 0.0 0.0 0.0 90-91 1.725 0.0 0.0 0.0 0.0 92-93 2.0 0.0 0.0 0.0 0.0 94-95 2.4375 0.0 0.0 0.0 0.0 96-97 2.9 0.0 0.0 0.0 0.0 98-99 3.3875 0.0 0.0 0.0 0.0 100-101 3.825 0.0 0.0 0.0 0.0 102-103 4.2375 0.0 0.0 0.0 0.0 104-105 4.75 0.0 0.0 0.0 0.0 106-107 5.25 0.0 0.0 0.0 0.0 108-109 5.949999999999999 0.0 0.0 0.0 0.0 110-111 6.6875 0.0 0.0 0.0 0.0 112-113 7.4125 0.0 0.0 0.0 0.0 114-115 8.3625 0.0 0.0 0.0 0.0 116-117 9.05 0.0 0.0 0.0 0.0 118-119 9.8 0.0 0.0 0.0 0.0 120-121 10.3125 0.0 0.0 0.0 0.0 122-123 11.075 0.0 0.0 0.0 0.0 124-125 11.7375 0.0 0.0 0.0 0.0 126-127 12.774999999999999 0.0 0.0 0.0 0.0 128-129 13.837499999999999 0.0 0.0 0.0 0.0 130-131 14.8125 0.0 0.0 0.0 0.0 132-133 15.6375 0.0 0.0 0.0 0.0 134-135 16.375 0.0 0.0 0.0 0.0 136-137 17.2375 0.0 0.0 0.0 0.0 138-139 18.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTGAT 10 0.006843168 144.91249 7 >>END_MODULE SRR7473368 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7473368_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.01925 34.0 33.0 34.0 32.0 34.0 2 33.1015 34.0 33.0 34.0 33.0 34.0 3 32.923 34.0 33.0 34.0 33.0 34.0 4 32.84975 34.0 33.0 34.0 33.0 34.0 5 32.93775 34.0 33.0 34.0 33.0 34.0 6 36.933 38.0 38.0 38.0 37.0 38.0 7 36.79425 38.0 38.0 38.0 36.0 38.0 8 36.888 38.0 38.0 38.0 37.0 38.0 9 36.90225 38.0 38.0 38.0 37.0 38.0 10-14 36.845 38.0 38.0 38.0 37.0 38.0 15-19 36.897450000000006 38.0 38.0 38.0 37.0 38.0 20-24 36.92605 38.0 38.0 38.0 37.0 38.0 25-29 36.9159 38.0 38.0 38.0 37.2 38.0 30-34 36.900749999999995 38.0 38.0 38.0 37.0 38.0 35-39 36.734449999999995 38.0 38.0 38.0 36.6 38.0 40-44 36.85975 38.0 38.0 38.0 37.0 38.0 45-49 36.763400000000004 38.0 38.0 38.0 37.0 38.0 50-54 36.823 38.0 38.0 38.0 37.0 38.0 55-59 36.90175 38.0 38.0 38.0 37.0 38.0 60-64 36.901799999999994 38.0 38.0 38.0 37.0 38.0 65-69 36.533249999999995 38.0 38.0 38.0 36.0 38.0 70-74 36.156 38.0 38.0 38.0 35.8 38.0 75-79 36.196450000000006 38.0 38.0 38.0 36.0 38.0 80-84 36.06775 38.0 38.0 38.0 35.2 38.0 85-89 36.06335 38.0 38.0 38.0 35.0 38.0 90-94 35.942899999999995 38.0 38.0 38.0 34.8 38.0 95-99 35.92509999999999 38.0 38.0 38.0 34.6 38.0 100-104 35.75425 38.0 38.0 38.0 34.0 38.0 105-109 35.54285 38.0 38.0 38.0 33.4 38.0 110-114 35.26405 38.0 38.0 38.0 31.8 38.0 115-119 34.94985 38.0 37.2 38.0 29.0 38.0 120-124 35.09905 38.0 38.0 38.0 30.6 38.0 125-129 34.92555 38.0 37.4 38.0 30.0 38.0 130-134 34.46085 38.0 36.0 38.0 25.8 38.0 135-139 34.154 38.0 35.4 38.0 24.4 38.0 140-144 33.495099999999994 38.0 34.0 38.0 19.8 38.0 145-149 32.664249999999996 38.0 33.0 38.0 10.8 38.0 150-151 28.1595 35.0 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 5.0 4 3.0 5 3.0 6 5.0 7 1.0 8 4.0 9 1.0 10 5.0 11 17.0 12 5.0 13 1.0 14 6.0 15 3.0 16 7.0 17 46.0 18 7.0 19 6.0 20 8.0 21 5.0 22 7.0 23 12.0 24 8.0 25 12.0 26 16.0 27 24.0 28 29.0 29 22.0 30 29.0 31 45.0 32 49.0 33 72.0 34 89.0 35 199.0 36 495.0 37 2731.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.175000000000004 16.5 13.475000000000001 26.85 2 30.988735919899874 22.22778473091364 25.081351689612013 21.70212765957447 3 27.669172932330827 24.411027568922307 25.68922305764411 22.230576441102755 4 28.66098294884654 30.71715145436309 19.207622868605817 21.414242728184554 5 29.416186419443747 32.548233525432224 17.263843648208468 20.77173640691556 6 25.45 34.375 18.725 21.45 7 24.175 19.425 31.65 24.75 8 27.01350675337669 23.761880940470235 21.010505252626313 28.214107053526767 9 27.175 24.05 24.099999999999998 24.675 10-14 29.107652269656175 25.058805865572293 21.45538261348281 24.378159251288725 15-19 29.12184138103578 24.428321240930696 23.387540655491616 23.062296722541905 20-24 29.997499374843713 25.166291572893222 22.12553138284571 22.710677669417354 25-29 27.791675005003004 26.505903542125274 23.138883329998 22.563538122873723 30-34 28.77287728772877 26.07760776077608 22.822282228222825 22.327232723272328 35-39 28.967380428256956 25.330198118871323 23.298979387632578 22.403442065239144 40-44 29.510328615015258 25.543940379132696 23.378182363827342 21.567548642024708 45-49 28.709354677338673 25.68784392196098 23.121560780390197 22.481240620310157 50-54 27.72579434575932 25.589191893920436 23.867900925694272 22.81711283462597 55-59 27.68384192096048 25.992996498249127 24.482241120560282 21.840920460230116 60-64 26.886886886886884 25.965965965965964 24.0990990990991 23.04804804804805 65-69 28.09135072870236 26.273351029198178 24.2199629388491 21.415335303250362 70-74 28.75444127508382 26.242305960066055 23.980383325826953 21.02286943902317 75-79 27.423711855927962 26.313156578289142 24.27713856928464 21.98599299649825 80-84 28.647188312987794 25.79047428457074 24.139483690214128 21.422853712227337 85-89 28.225 26.005 24.279999999999998 21.490000000000002 90-94 27.676605963578147 26.5259155493296 24.174504702821693 21.622973784270563 95-99 28.132033008252062 26.846711677919483 24.001000250062514 21.02025506376594 100-104 29.025000000000002 26.325 23.74 20.91 105-109 26.875156367275455 27.965974480860645 23.54265699274456 21.61621215911934 110-114 28.658781074578986 27.049919807538092 23.536487570168404 20.754811547714517 115-119 28.706118355065197 27.472417251755267 23.570712136409227 20.250752256770312 120-124 28.78234322606476 27.456083279115163 23.347179820829787 20.41439367399029 125-129 28.765 26.735 23.775 20.724999999999998 130-134 28.73 26.68 23.544999999999998 21.044999999999998 135-139 29.93 26.245 23.185 20.64 140-144 29.125 26.665 23.41 20.8 145-149 29.365000000000002 27.229999999999997 23.3 20.105 150-151 29.775000000000002 28.5625 22.5 19.162499999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.5 22 2.0 23 1.0 24 0.0 25 2.0 26 3.5 27 3.0 28 2.0 29 2.0 30 5.5 31 8.0 32 10.0 33 18.5 34 31.5 35 38.5 36 40.5 37 45.0 38 56.5 39 75.0 40 83.0 41 89.5 42 104.5 43 109.5 44 116.5 45 129.5 46 135.5 47 129.0 48 135.5 49 158.0 50 167.0 51 163.0 52 196.5 53 271.0 54 271.0 55 238.5 56 219.0 57 177.0 58 128.0 59 92.5 60 74.0 61 76.0 62 87.5 63 62.5 64 35.0 65 25.5 66 24.0 67 26.5 68 25.5 69 24.5 70 20.0 71 10.0 72 7.0 73 8.0 74 7.0 75 5.5 76 4.5 77 4.5 78 4.0 79 3.0 80 1.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.25 4 0.3 5 0.22499999999999998 6 0.0 7 0.0 8 0.05 9 0.0 10-14 0.095 15-19 0.075 20-24 0.025 25-29 0.06 30-34 0.01 35-39 0.06 40-44 0.034999999999999996 45-49 0.05 50-54 0.075 55-59 0.05 60-64 0.1 65-69 0.165 70-74 0.08499999999999999 75-79 0.05 80-84 0.06 85-89 0.0 90-94 0.06 95-99 0.025 100-104 0.0 105-109 0.075 110-114 0.24 115-119 0.3 120-124 0.095 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.425 #Duplication Level Percentage of deduplicated Percentage of total 1 91.23445556566577 75.2 2 5.156202608431908 8.5 3 1.5468607825295724 3.8249999999999997 4 0.5762814680012133 1.9 5 0.6066120715802245 2.5 6 0.21231422505307856 1.05 7 0.24264482863208978 1.4000000000000001 8 0.060661207158022444 0.4 9 0.060661207158022444 0.44999999999999996 >10 0.3033060357901122 4.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 50 1.25 Illumina Single End PCR Primer 1 (100% over 50bp) GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA 20 0.5 No Hit GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT 20 0.5 No Hit GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA 18 0.44999999999999996 No Hit CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG 18 0.44999999999999996 No Hit CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA 17 0.42500000000000004 No Hit GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG 16 0.4 No Hit GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG 11 0.27499999999999997 No Hit GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA 11 0.27499999999999997 No Hit GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG 10 0.25 No Hit GGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTA 9 0.22499999999999998 No Hit GCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGC 9 0.22499999999999998 No Hit GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG 8 0.2 No Hit CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT 8 0.2 No Hit GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA 7 0.17500000000000002 No Hit GGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAG 7 0.17500000000000002 No Hit ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA 7 0.17500000000000002 No Hit CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA 7 0.17500000000000002 No Hit CTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG 7 0.17500000000000002 No Hit GCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGCCCTG 7 0.17500000000000002 No Hit ACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCA 7 0.17500000000000002 No Hit CGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCT 7 0.17500000000000002 No Hit TGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAG 6 0.15 No Hit CCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGG 6 0.15 No Hit GTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATC 6 0.15 No Hit CAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCC 6 0.15 No Hit AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG 6 0.15 No Hit CAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACA 6 0.15 No Hit GGGAGACACACGGCGGGTGCTAACGTCCGTCGTGAAGAGGGAAACAACCC 6 0.15 No Hit AGTCGGCCTGCGCGGAAGATGTAACGGGGCTAAACCATGCACCGAAGCTG 5 0.125 No Hit GTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT 5 0.125 No Hit GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT 5 0.125 No Hit TCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTA 5 0.125 No Hit GCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGA 5 0.125 No Hit CCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT 5 0.125 No Hit GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTG 5 0.125 No Hit TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA 5 0.125 No Hit AAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA 5 0.125 No Hit CGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAG 5 0.125 No Hit GCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTCGTGAATTCATCTCC 5 0.125 No Hit CCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCT 5 0.125 No Hit GGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCT 5 0.125 No Hit CAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAG 5 0.125 No Hit GGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTA 5 0.125 No Hit GCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGC 5 0.125 No Hit AGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGC 5 0.125 No Hit CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA 5 0.125 No Hit CGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAAT 5 0.125 No Hit GGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.2875 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.4125 0.0 0.0 0.0 0.0 80-81 0.5 0.0 0.0 0.0 0.0 82-83 0.7124999999999999 0.0 0.0 0.0 0.0 84-85 0.9750000000000001 0.0 0.0 0.0 0.0 86-87 1.25 0.0 0.0 0.0 0.0 88-89 1.4375 0.0 0.0 0.0 0.0 90-91 1.6875 0.0 0.0 0.0 0.0 92-93 1.9875 0.0 0.0 0.0 0.0 94-95 2.4 0.0 0.0 0.0 0.0 96-97 2.85 0.0 0.0 0.0 0.0 98-99 3.3 0.0 0.0 0.0 0.0 100-101 3.7125000000000004 0.0 0.0 0.0 0.0 102-103 4.1 0.0 0.0 0.0 0.0 104-105 4.6 0.0 0.0 0.0 0.0 106-107 5.1 0.0 0.0 0.0 0.0 108-109 5.762499999999999 0.0 0.0 0.0 0.0 110-111 6.45 0.0 0.0 0.0 0.0 112-113 7.125 0.0 0.0 0.0 0.0 114-115 8.0 0.0 0.0 0.0 0.0 116-117 8.6375 0.0 0.0 0.0 0.0 118-119 9.3375 0.0 0.0 0.0 0.0 120-121 9.875 0.0 0.0 0.0 0.0 122-123 10.5625 0.0 0.0 0.0 0.0 124-125 11.225 0.0 0.0 0.0 0.0 126-127 12.2875 0.0 0.0 0.0 0.0 128-129 13.225 0.0 0.0 0.0 0.0 130-131 14.1125 0.0 0.0 0.0 0.0 132-133 14.875 0.0 0.0 0.0 0.0 134-135 15.6125 0.0 0.0 0.0 0.0 136-137 16.5 0.0 0.0 0.0 0.0 138-139 17.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAGATT 10 0.006630061 146.43037 2 CAGATTG 10 0.006630061 146.43037 3 AGATTGA 10 0.006630061 146.43037 4 GCAGCCA 10 0.0068874825 144.6 8 TTGAACG 10 0.0068874825 144.6 7 TGAACGC 10 0.0068874825 144.6 8 CGGTAAT 10 0.0068874825 144.6 145 AAAAAAA 160 3.5786108E-4 18.075 145 >>END_MODULE Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784790 spots for SRR7473368.sra Written 784790 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra Read 784777 spots for SRR7473368.sra Written 784777 spots for SRR7473368.sra SRR ids: ['SRR7473368.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_2tekt137 SRR7473368.sra spots: 15695553 blocks: [[1, 784777], [784778, 1569554], [1569555, 2354331], [2354332, 3139108], [3139109, 3923885], [3923886, 4708662], [4708663, 5493439], [5493440, 6278216], [6278217, 7062993], [7062994, 7847770], [7847771, 8632547], [8632548, 9417324], [9417325, 10202101], [10202102, 10986878], [10986879, 11771655], [11771656, 12556432], [12556433, 13341209], [13341210, 14125986], [14125987, 14910763], [14910764, 15695553]] SRR7473368 file size 5297007 SRR7473368 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7473368 SRR7473368_1.fastq SRR7473368_2.fastq Input file: SRR7473368_1.fastq Paired file: SRR7473368_2.fastq trimmed: SRR7473368-trimmed-pair1.fastq, SRR7473368-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 15:22:52 2024 >> started Sat Dec 7 15:23:09 2024 >> done (16.694s) 15695553 read pairs processed; of these: 56275 ( 0.36%) short read pairs filtered out after trimming by size control 289139 ( 1.84%) empty read pairs filtered out after trimming by size control 15350139 (97.80%) read pairs available; of these: 8050771 (52.45%) trimmed read pairs available after processing 7299368 (47.55%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 14 0.00% 20 11 0.00% 21 12 0.00% 22 21 0.00% 23 17 0.00% 24 38 0.00% 25 23 0.00% 26 28 0.00% 27 34 0.00% 28 50 0.00% 29 83 0.00% 30 52 0.00% 31 77 0.00% 32 82 0.00% 33 58 0.00% 34 103 0.00% 35 123 0.00% 36 93 0.00% 37 97 0.00% 38 117 0.00% 39 121 0.00% 40 147 0.00% 41 191 0.00% 42 192 0.00% 43 175 0.00% 44 220 0.00% 45 257 0.00% 46 298 0.00% 47 361 0.00% 48 385 0.00% 49 436 0.00% 50 532 0.00% 51 569 0.00% 52 611 0.00% 53 596 0.00% 54 742 0.00% 55 742 0.00% 56 783 0.01% 57 835 0.01% 58 941 0.01% 59 1118 0.01% 60 1376 0.01% 61 1651 0.01% 62 1605 0.01% 63 1854 0.01% 64 2031 0.01% 65 2475 0.02% 66 2620 0.02% 67 3353 0.02% 68 5651 0.04% 69 26383 0.17% 70 45704 0.30% 71 21010 0.14% 72 11761 0.08% 73 9395 0.06% 74 8696 0.06% 75 7784 0.05% 76 7707 0.05% 77 8231 0.05% 78 8675 0.06% 79 9540 0.06% 80 10649 0.07% 81 11718 0.08% 82 13671 0.09% 83 16066 0.10% 84 21348 0.14% 85 22445 0.15% 86 23904 0.16% 87 23949 0.16% 88 26815 0.17% 89 26722 0.17% 90 28850 0.19% 91 31117 0.20% 92 30834 0.20% 93 37901 0.25% 94 38719 0.25% 95 43019 0.28% 96 41507 0.27% 97 41675 0.27% 98 40396 0.26% 99 41075 0.27% 100 45691 0.30% 101 42295 0.28% 102 45236 0.29% 103 46892 0.31% 104 50653 0.33% 105 57623 0.38% 106 52946 0.34% 107 50864 0.33% 108 54285 0.35% 109 63728 0.42% 110 64891 0.42% 111 55595 0.36% 112 57932 0.38% 113 69328 0.45% 114 62394 0.41% 115 68824 0.45% 116 69793 0.45% 117 64654 0.42% 118 66592 0.43% 119 65926 0.43% 120 69019 0.45% 121 64839 0.42% 122 70069 0.46% 123 73204 0.48% 124 74624 0.49% 125 75513 0.49% 126 75378 0.49% 127 77277 0.50% 128 75838 0.49% 129 75413 0.49% 130 78137 0.51% 131 78044 0.51% 132 79620 0.52% 133 83868 0.55% 134 89868 0.59% 135 92231 0.60% 136 93078 0.61% 137 100373 0.65% 138 99891 0.65% 139 99762 0.65% 140 98112 0.64% 141 108828 0.71% 142 108127 0.70% 143 113813 0.74% 144 123746 0.81% 145 136051 0.89% 146 154598 1.01% 147 186855 1.22% 148 256674 1.67% 149 465528 3.03% 150 2744978 17.88% 151 7299368 47.55% 15350139 reads passed initial QC criterion=sequence-density sequence-density=0.80 sequence-density-rank=1 fanout-score=1.74 fanout-score-rank=39 prefix-density=1.39 prefix-fanout=1.0 sequence=TAATTTTTCAACATTAGTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGGCTCCCCTATTCGGTTAACCTTGCTACAGAATATAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACACGCCTAAGCGTGCTCCCACTGCTTGTACGTACACGGTTTCAGGTTCTTTTTCACTCCCCTCGCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG criterion=fanout-score sequence-density=0.15 sequence-density-rank=32 fanout-score=11.57 fanout-score-rank=1 prefix-density=1.76 prefix-fanout=1.0 sequence=CCATCAGGCAGCTTCCCAGACAT criterion=sequence-density sequence-density=1.02 sequence-density-rank=1 fanout-score=3.15 fanout-score-rank=26 prefix-density=3.21 prefix-fanout=1.0 sequence=GGTAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTG criterion=fanout-score sequence-density=0.07 sequence-density-rank=36 fanout-score=25.19 fanout-score-rank=1 prefix-density=1.67 prefix-fanout=1.0 sequence=CGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG SRR7473368 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 15:24:38 Started mapping on | Dec 07 15:24:39 Finished on | Dec 07 15:39:54 Mapping speed, Million of reads per hour | 60.39 Number of input reads | 15350139 Average input read length | 283 UNIQUE READS: Uniquely mapped reads number | 8547127 Uniquely mapped reads % | 55.68% Average mapped length | 287.34 Number of splices: Total | 6003791 Number of splices: Annotated (sjdb) | 5624479 Number of splices: GT/AG | 5921376 Number of splices: GC/AG | 71410 Number of splices: AT/AC | 3261 Number of splices: Non-canonical | 7744 Mismatch rate per base, % | 0.13% Deletion rate per base | 0.01% Deletion average length | 1.49 Insertion rate per base | 0.01% Insertion average length | 1.14 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 131990 % of reads mapped to multiple loci | 0.86% Number of reads mapped to too many loci | 23203 % of reads mapped to too many loci | 0.15% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 40.22% % of reads unmapped: other | 3.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6695351 6695351 6695351 N_multimapping 131990 131990 131990 N_noFeature 242100 8245893 318539 N_ambiguous 250294 996 26367 UnstrandedReadsAssigned:8054733 PositiveStrandReadsAssigned:300238 NegativeStrandReadsAssigned:8202221 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=136 echo kmer=131 SRR7473368 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7473368-trimmed-pair1.fastq SRR7473368-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,350,139 reads, 8,412,858 reads pseudoaligned [quant] estimated average fragment length: 217.166 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,051 rounds 52973 SRR7473368.ke.tsv 35125 SRR7473368.se.tsv 88098 total ==> SRR7473368.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 719.975 0 0 PNS24247 1044 827.834 9.76854 1.86795 PNS24249 1928 1711.83 7.2777 0.672993 PNS24246 1044 827.834 9.76854 1.86795 PNS24248 1044 827.834 9.76854 1.86795 PNS24244 1471 1254.83 93.4167 11.7846 PNS24243 293 111.215 0 0 KQK14069 1603 1386.83 411.775 47.0017 KQK14071 474 267.323 9.91555 5.87163 ==> SRR7473368.se.tsv <== BRADI_1g14170v3 576 BRADI_1g53295v3 2 BRADI_1g59795v3 149 BRADI_1g07683v3 0 BRADI_1g00485v3 16 BRADI_1g20270v3 694 BRADI_1g74790v3 71 BRADI_1g09890v3 0 BRADI_1g77505v3 148 BRADI_1g48960v3 0 SRR7473368 completed mapping pipeline successfully