Starting /dee2/code/volunteer_pipeline.sh SRR7692619 current disk space = 2825397784576 free memory = 1575303444 SRR7692619_1.fastq is conventional basespace SRR7692619_1.fastq read1 length is 126 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7692619_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9508772 Sequences flagged as poor quality 0 Sequence length 126 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.023672036725667 27.0 18.0 32.0 18.0 33.0 2 28.363824371853696 29.0 27.0 31.0 18.0 33.0 3 27.039841737713346 29.0 25.0 31.0 18.0 33.0 4 30.301196621393384 32.0 30.0 33.0 27.0 33.0 5 31.494782396717472 33.0 32.0 33.0 30.0 33.0 6 35.60360990882945 37.0 35.0 38.0 31.0 38.0 7 36.638499166874546 38.0 37.0 38.0 34.0 38.0 8 36.649893382657616 38.0 37.0 38.0 34.0 38.0 9 37.04754809559005 38.0 38.0 38.0 35.0 38.0 10-11 37.18782041466554 38.0 38.0 38.0 36.0 38.0 12-13 37.2986865706739 38.0 38.0 38.0 36.0 38.0 14-15 37.3350953204052 38.0 38.0 38.0 36.5 38.0 16-17 37.3040880567964 38.0 38.0 38.0 36.5 38.0 18-19 37.393108384552704 38.0 38.0 38.0 37.0 38.0 20-21 37.42001748490762 38.0 38.0 38.0 37.0 38.0 22-23 37.42124713895758 38.0 38.0 38.0 37.0 38.0 24-25 37.39740247215939 38.0 38.0 38.0 37.0 38.0 26-27 37.399001101298886 38.0 38.0 38.0 37.0 38.0 28-29 37.40985150343283 38.0 38.0 38.0 37.0 38.0 30-31 37.410630363205684 38.0 38.0 38.0 37.0 38.0 32-33 37.41390791576452 38.0 38.0 38.0 37.0 38.0 34-35 37.342719017765916 38.0 38.0 38.0 37.0 38.0 36-37 37.38115079423505 38.0 38.0 38.0 37.0 38.0 38-39 37.381914720428675 38.0 38.0 38.0 37.0 38.0 40-41 37.38708116042744 38.0 38.0 38.0 37.0 38.0 42-43 37.3712961568539 38.0 38.0 38.0 37.0 38.0 44-45 37.37402300738728 38.0 38.0 38.0 37.0 38.0 46-47 37.33492900029573 38.0 38.0 38.0 37.0 38.0 48-49 37.32851339794455 38.0 38.0 38.0 37.0 38.0 50-51 37.31271808809802 38.0 38.0 38.0 37.0 38.0 52-53 37.29423326166618 38.0 38.0 38.0 37.0 38.0 54-55 37.29601451165303 38.0 38.0 38.0 37.0 38.0 56-57 37.28153099054221 38.0 38.0 38.0 37.0 38.0 58-59 37.26345736336931 38.0 38.0 38.0 37.0 38.0 60-61 37.25204584777088 38.0 38.0 38.0 36.0 38.0 62-63 37.24105972884827 38.0 38.0 38.0 36.0 38.0 64-65 37.213985044546234 38.0 38.0 38.0 36.0 38.0 66-67 37.1947167310353 38.0 38.0 38.0 36.0 38.0 68-69 37.18350119237269 38.0 38.0 38.0 36.0 38.0 70-71 37.16398510764587 38.0 38.0 38.0 36.0 38.0 72-73 37.1548506999642 38.0 38.0 38.0 36.0 38.0 74-75 37.14322795835256 38.0 38.0 38.0 36.0 38.0 76-77 37.121212865341604 38.0 38.0 38.0 36.0 38.0 78-79 37.104301375614014 38.0 38.0 38.0 36.0 38.0 80-81 37.07717663227176 38.0 38.0 38.0 36.0 38.0 82-83 37.069495356498194 38.0 38.0 38.0 36.0 38.0 84-85 37.04203660577833 38.0 38.0 38.0 36.0 38.0 86-87 37.023394713849484 38.0 38.0 38.0 36.0 38.0 88-89 37.0035378911178 38.0 38.0 38.0 36.0 38.0 90-91 36.978667487242305 38.0 38.0 38.0 35.0 38.0 92-93 36.95792926783816 38.0 38.0 38.0 35.0 38.0 94-95 36.93154599773767 38.0 38.0 38.0 35.0 38.0 96-97 36.89559298508787 38.0 38.0 38.0 35.0 38.0 98-99 36.85244556289708 38.0 38.0 38.0 35.0 38.0 100-101 36.8109637606202 38.0 38.0 38.0 35.0 38.0 102-103 36.785344889960555 38.0 38.0 38.0 35.0 38.0 104-105 36.74501481368993 38.0 38.0 38.0 35.0 38.0 106-107 36.69716362954123 38.0 38.0 38.0 34.0 38.0 108-109 36.653934388162845 38.0 38.0 38.0 34.0 38.0 110-111 36.619291481591944 38.0 38.0 38.0 34.0 38.0 112-113 36.55505753003648 38.0 38.0 38.0 34.0 38.0 114-115 36.48083706287205 38.0 38.0 38.0 34.0 38.0 116-117 36.449167516057805 38.0 38.0 38.0 34.0 38.0 118-119 36.395030451881695 38.0 38.0 38.0 34.0 38.0 120-121 36.33264368942699 38.0 37.5 38.0 34.0 38.0 122-123 36.29688160574257 38.0 37.0 38.0 33.5 38.0 124-125 36.228963529675546 38.0 37.0 38.0 33.0 38.0 126 32.740639590474984 36.0 31.0 38.0 15.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -2.9614329187315533 1101 2 -1.2991312281217375 1101 3 -1.2362069669804896 1101 4 -1.0209387374130259 1101 5 -0.5174468926912148 1101 6 -0.19154234972876338 1101 7 0.03815719510431137 1101 8 0.05678891504871331 1101 9 0.08547717742867178 1101 10-11 0.12498207586099852 1101 12-13 0.09786850362906563 1101 14-15 0.1142410679748167 1101 16-17 0.14815187637000093 1101 18-19 0.11424893532083047 1101 20-21 0.0782444600863883 1101 22-23 0.12169539601246271 1101 24-25 0.09724216771493843 1101 26-27 0.048265969313028734 1101 28-29 0.07707386757799384 1101 30-31 0.06335560721856126 1101 32-33 0.02989198762638523 1101 34-35 0.07787986833525906 1101 36-37 0.05173773454438191 1101 38-39 0.07485574610829815 1101 40-41 0.059552649881801756 1101 42-43 0.027138444384029015 1101 44-45 0.05428962755000555 1101 46-47 -0.009406838793275085 1101 48-49 0.003839579575021901 1101 50-51 0.02799859442695407 1101 52-53 0.03840549883366151 1101 54-55 0.06396103280435028 1101 56-57 0.0224624580161219 1101 58-59 0.045756711809282535 1101 60-61 0.09010430172288153 1101 62-63 0.07181406973973736 1101 64-65 0.07027445187666359 1101 66-67 0.009318021035326751 1101 68-69 0.02300674297674732 1101 70-71 -0.015749740221700392 1101 72-73 0.008048514032346077 1101 74-75 0.0193127605743868 1101 76-77 0.04642412255127937 1101 78-79 0.051460496969070846 1101 80-81 0.03071352155304652 1101 82-83 0.03131184858566627 1101 84-85 0.019604679480160314 1101 86-87 0.04491977106279421 1101 88-89 -0.050949561907657426 1101 90-91 -0.012493376834164849 1101 92-93 -0.005618714421125048 1101 94-95 -0.01310974316432123 1101 96-97 -0.04623104567696856 1101 98-99 -0.03594146844323376 1101 100-101 -0.044267056324073906 1101 102-103 -0.039854816228597656 1101 104-105 -0.008098785263797481 1101 106-107 -0.029541928639609694 1101 108-109 -0.006475613187127749 1101 110-111 -0.027396912383053973 1101 112-113 -0.05166775104643051 1101 114-115 -0.009930321572284129 1101 116-117 -0.051378611106208893 1101 118-119 -0.0693368373652632 1101 120-121 -0.048004260827319456 1101 122-123 -0.13187435558382532 1101 124-125 -0.0775950065780151 1101 126 -0.16593061578113577 1102 1 -3.4783281243918367 1102 2 -1.579390091993833 1102 3 -1.4666751073624944 1102 4 -1.0522910573750615 1102 5 -0.5726644713541589 1102 6 -0.33128615418432616 1102 7 -0.05175777817237304 1102 8 -0.07909967471530166 1102 9 -0.02074313869484712 1102 10-11 0.08518567174266423 1102 12-13 0.09164867245097952 1102 14-15 0.08828461178912761 1102 16-17 0.15030999068562068 1102 18-19 0.03852315527201 1102 20-21 0.054251266013601196 1102 22-23 0.06393934792527745 1102 24-25 0.040810793188988725 1102 26-27 0.016614900408718825 1102 28-29 0.04234566765145331 1102 30-31 0.03632382964655534 1102 32-33 -0.007720831263299033 1102 34-35 0.003395672650526649 1102 36-37 -0.006648934328225664 1102 38-39 -0.022002777377217342 1102 40-41 0.018928596159774713 1102 42-43 0.02901447270799906 1102 44-45 -0.002138515886024095 1102 46-47 -0.005044991457928916 1102 48-49 0.02483532028800539 1102 50-51 -0.02476514906759064 1102 52-53 -0.013927827394660142 1102 54-55 -0.028928595757903963 1102 56-57 -0.013822907334436252 1102 58-59 0.006942683082236556 1102 60-61 -0.024289707644683745 1102 62-63 0.008578769817340515 1102 64-65 -0.03176355386080587 1102 66-67 -0.004981649528829735 1102 68-69 -0.006369723207832578 1102 70-71 0.03572523822924012 1102 72-73 -0.027512461258325516 1102 74-75 -0.030000680748408115 1102 76-77 -0.030712792318347226 1102 78-79 -0.057755062065311336 1102 80-81 -0.05351441768711851 1102 82-83 -0.05958864658872898 1102 84-85 -0.018559736644974123 1102 86-87 -0.0616897757985555 1102 88-89 -0.04100850195001726 1102 90-91 -0.09517698478758518 1102 92-93 -0.08631276606916316 1102 94-95 -0.07221085507652703 1102 96-97 -0.12263825864256006 1102 98-99 -0.09012423558021965 1102 100-101 -0.08804751272990075 1102 102-103 -0.13721944230127292 1102 104-105 -0.14767479296067165 1102 106-107 -0.06699891651721401 1102 108-109 -0.04107583263057535 1102 110-111 -0.055033834188535025 1102 112-113 -0.10386522488672512 1102 114-115 -0.13518509554992875 1102 116-117 -0.12104123682408385 1102 118-119 -0.08151070451300058 1102 120-121 -0.09706753797352263 1102 122-123 -0.1785663341465309 1102 124-125 -0.10591035012745209 1102 126 -0.332842213724831 1103 1 -3.4894905324597545 1103 2 -1.3851668271272217 1103 3 -1.1024788902097917 1103 4 -1.1514691794858507 1103 5 -0.4568950758345949 1103 6 -0.21483537103007677 1103 7 0.09792110433460977 1103 8 0.08149900280686495 1103 9 0.09570233018662577 1103 10-11 0.13509623492151945 1103 12-13 0.1354414322902997 1103 14-15 0.1621893839810511 1103 16-17 0.185356840153716 1103 18-19 0.11687271921373821 1103 20-21 0.08174836597967072 1103 22-23 0.0809462555012388 1103 24-25 0.04757659072967613 1103 26-27 0.07194415556684675 1103 28-29 0.05457331999077297 1103 30-31 0.08753747071794038 1103 32-33 0.08426842826141012 1103 34-35 0.0702965235008719 1103 36-37 0.02382108590173715 1103 38-39 0.06085958848832007 1103 40-41 0.06373535793576934 1103 42-43 0.031838012974567675 1103 44-45 0.043889521534374865 1103 46-47 -0.01365824415269401 1103 48-49 0.01647785822172665 1103 50-51 -0.02192388507543086 1103 52-53 -0.0205320858363649 1103 54-55 0.036168389492708286 1103 56-57 0.04611221779870078 1103 58-59 0.034600901102116666 1103 60-61 0.021748525896505555 1103 62-63 -0.01056735445393997 1103 64-65 -2.881176016771292E-4 1103 66-67 0.056490205115267145 1103 68-69 0.009980587190071333 1103 70-71 0.0130010948917203 1103 72-73 -0.0028099859975228014 1103 74-75 -0.05765520608836994 1103 76-77 -0.025076863792335757 1103 78-79 0.032246056321682204 1103 80-81 -0.019598688989603374 1103 82-83 -0.05175108995616284 1103 84-85 -0.023661233679028726 1103 86-87 -0.0800118577808746 1103 88-89 -0.09310104066779701 1103 90-91 -0.053996059153242015 1103 92-93 -0.06767243083891827 1103 94-95 -0.06705199348343172 1103 96-97 -0.0674171779534447 1103 98-99 -0.04825768341052594 1103 100-101 -0.09809683669182334 1103 102-103 -0.030885577927570296 1103 104-105 -0.0982731823513916 1103 106-107 7.932016037486278E-4 1103 108-109 -0.06307875973657673 1103 110-111 -0.12025252994127555 1103 112-113 -0.08808133972362953 1103 114-115 -0.07090520534303835 1103 116-117 -0.0561285783196368 1103 118-119 -0.0802793465437901 1103 120-121 -0.024550980144752543 1103 122-123 -0.09247770400241251 1103 124-125 -0.07794055299452651 1103 126 -0.16090386811962532 1104 1 -3.4938526545991735 1104 2 -1.3568563298878473 1104 3 -0.9577005636893752 1104 4 -0.934380851012655 1104 5 -0.34507294481492323 1104 6 -0.020529135000920462 1104 7 0.14952418558451797 1104 8 0.17117983205189802 1104 9 0.12399612241280522 1104 10-11 0.13583047138923376 1104 12-13 0.11294441601020111 1104 14-15 0.11566663816234524 1104 16-17 0.1730527456390618 1104 18-19 0.11253357499042238 1104 20-21 0.09045000735289221 1104 22-23 0.08674650881742707 1104 24-25 0.04792402091226222 1104 26-27 0.052365095989536314 1104 28-29 0.09533733638323127 1104 30-31 0.08440497391711688 1104 32-33 0.053264912435693645 1104 34-35 0.023540043864898053 1104 36-37 0.04238400339070125 1104 38-39 0.07326364034722843 1104 40-41 0.06617227844226647 1104 42-43 0.07102534885633816 1104 44-45 0.04615131213723345 1104 46-47 -0.010576169218232678 1104 48-49 0.025721151124834307 1104 50-51 0.02961408071487881 1104 52-53 0.040042439795804796 1104 54-55 0.05911166826814451 1104 56-57 0.052591240120420935 1104 58-59 0.028246859552588433 1104 60-61 0.013571694672499746 1104 62-63 0.024044376038155235 1104 64-65 0.041484779312163766 1104 66-67 -0.02169273400702565 1104 68-69 0.018186941968011183 1104 70-71 0.00948663285042528 1104 72-73 0.008898625934527615 1104 74-75 0.04238883378111069 1104 76-77 -0.02517992349618936 1104 78-79 0.05189790202791045 1104 80-81 0.06965145774177728 1104 82-83 0.009929512100690374 1104 84-85 -8.834636545884678E-5 1104 86-87 0.01444970759749964 1104 88-89 0.028043203643768777 1104 90-91 0.006715618046449379 1104 92-93 0.056259539256245716 1104 94-95 -0.0014251559319760077 1104 96-97 0.0378368146128949 1104 98-99 -0.013652055362030069 1104 100-101 0.020365673440281284 1104 102-103 0.0354326426189715 1104 104-105 0.00886544253010868 1104 106-107 0.03271041346750536 1104 108-109 0.0014425468600123281 1104 110-111 -0.019116016300706917 1104 112-113 -0.01618753124244421 1104 114-115 -0.03155487626109732 1104 116-117 -0.10166992165907374 1104 118-119 -0.009717541267782792 1104 120-121 0.10067821945465738 1104 122-123 -0.03089068411130569 1104 124-125 -0.004883257390254414 1104 126 -0.021000473551850973 1105 1 -3.040844611319823 1105 2 -1.3200872690846985 1105 3 -0.7286795294451593 1105 4 -0.3811182767420931 1105 5 -0.3066488863650605 1105 6 0.008135576914064302 1105 7 0.10437905593283858 1105 8 0.11612761759673873 1105 9 0.10212664696855 1105 10-11 0.09605995465670958 1105 12-13 0.06452884148799853 1105 14-15 0.08664087234507178 1105 16-17 0.11349224362640342 1105 18-19 0.058580941261297426 1105 20-21 0.03724445544747823 1105 22-23 0.06948988935329936 1105 24-25 0.03839167801101695 1105 26-27 0.047690611105828395 1105 28-29 0.05987518343984277 1105 30-31 0.04965416922066623 1105 32-33 0.027187002182643027 1105 34-35 0.02599351914279424 1105 36-37 0.002098344045009526 1105 38-39 0.026499049202520553 1105 40-41 0.01788361405412786 1105 42-43 -0.0057077032938934735 1105 44-45 -0.012361465610709388 1105 46-47 -0.014002282184243597 1105 48-49 -0.019115768201253047 1105 50-51 -0.05961437147767157 1105 52-53 -0.08413966930387318 1105 54-55 -0.02550017690582962 1105 56-57 -0.018997196994270382 1105 58-59 -0.04033041499815937 1105 60-61 -0.031513977729431986 1105 62-63 -0.021846519283393206 1105 64-65 -0.040287069104820716 1105 66-67 -0.014012515693956118 1105 68-69 -0.0405003906830288 1105 70-71 0.008553743008285153 1105 72-73 -0.03834771041965013 1105 74-75 -0.009904031243628708 1105 76-77 -0.02683199270061465 1105 78-79 -0.02902812012348477 1105 80-81 -0.0018402749694885756 1105 82-83 -0.08479983797200674 1105 84-85 -0.060990864641567555 1105 86-87 -0.023599082488601653 1105 88-89 -0.0752925421995485 1105 90-91 -0.07076221265825922 1105 92-93 -0.033163243050857716 1105 94-95 -0.035982617629272795 1105 96-97 -0.028809925484623022 1105 98-99 -0.03678734176236986 1105 100-101 -0.015972116653188095 1105 102-103 -0.02677694803612951 1105 104-105 -0.041599219002506516 1105 106-107 0.016465521460553134 1105 108-109 -0.05306899159747758 1105 110-111 -0.0914040549893187 1105 112-113 -0.08474236537021085 1105 114-115 -0.10241146603279105 1105 116-117 -0.0945118215745211 1105 118-119 -0.15977523487138257 1105 120-121 -0.01671082482813091 1105 122-123 0.0040543190843109755 1105 124-125 -0.06083012741847682 1105 126 -0.03985062627834424 1106 1 -2.806289445434807 1106 2 -1.1623507269304696 1106 3 -0.6287216009469994 1106 4 -0.7164749468001439 1106 5 -0.4579269941179014 1106 6 -0.09009088537229104 1106 7 0.04650303880477935 1106 8 0.17431481598056564 1106 9 0.11244691128397477 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2316 74-75 0.05844859510174416 2316 76-77 -0.012671957489395425 2316 78-79 0.04256914016945501 2316 80-81 0.06602422326920276 2316 82-83 0.07912913869604665 2316 84-85 0.08657290525844274 2316 86-87 0.10861397064824274 2316 88-89 0.13916351889503886 2316 90-91 0.10827532840919218 2316 92-93 0.12183012022525475 2316 94-95 0.10209093816451542 2316 96-97 0.11815252020156208 2316 98-99 0.11192165061466142 2316 100-101 0.14023456739630547 2316 102-103 0.15528190551008692 2316 104-105 0.16222855552891247 2316 106-107 0.19298002365809452 2316 108-109 0.18284948793795763 2316 110-111 0.13934587825207956 2316 112-113 0.11367860914591432 2316 114-115 0.16396789024864233 2316 116-117 0.21463841284076324 2316 118-119 0.16048644683139912 2316 120-121 0.15989292467249783 2316 122-123 0.07302076336277707 2316 124-125 0.16769295841577758 2316 126 0.8677885153700302 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 54.0 16 69.0 17 22.0 18 30.0 19 89.0 20 304.0 21 805.0 22 1950.0 23 3954.0 24 7018.0 25 11604.0 26 17807.0 27 26002.0 28 36263.0 29 49734.0 30 67301.0 31 91775.0 32 127423.0 33 182930.0 34 285264.0 35 530008.0 36 1631087.0 37 6437279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.80147111537774 10.45652876271535 9.150282486113612 44.5917176357933 2 23.68525969337348 13.32091979540279 35.880492995793944 27.113327515429788 3 23.05749890732473 16.514393236056137 23.483884144030377 36.94422371258875 4 26.418563827169272 23.692249640647603 21.55787308813378 28.33131344404935 5 26.47863467543443 28.376766211241577 23.831247610101496 21.313351503222496 6 22.06326605495325 31.69729493019078 24.235350338600405 22.004088676255567 7 18.438437686801198 23.410099642729893 38.38949971668266 19.761962953786252 8 20.894990436199333 23.115845032355388 29.831570259545607 26.157594271899676 9 20.34919966531956 21.70022585461088 33.159234441629266 24.791340038440296 10-11 23.64822790953744 29.313485513506865 23.430495383956877 23.60779119299882 12-13 23.482984932793837 23.103477124023538 26.847370849006815 26.566167094175807 14-15 22.899702506275258 24.721746404267556 26.972320926403537 25.40623016305365 16-17 23.575809789108416 24.92974907800923 26.075080988375788 25.41936014450657 18-19 23.40465204129408 25.242712728836068 25.927112354781457 25.42552287508839 20-21 23.436364863938266 25.26714280245651 26.149391319930693 25.147101013674533 22-23 23.436039752567353 25.458851431828272 25.904256065939972 25.200852749664403 24-25 23.277145654922606 25.075972513148166 25.846375914999392 25.800505916929843 26-27 23.19483906494783 25.317702224720122 26.076470862914096 25.410987847417953 28-29 23.445319050314566 25.361763706026913 25.778817407481707 25.414099836176817 30-31 23.363962263873145 25.185076591431528 25.680445811790932 25.7705153329044 32-33 23.331105390428196 25.212894220878056 26.02618014324984 25.429820245443903 34-35 23.550994013419242 25.256240445824986 25.75035035486334 25.442415185892436 36-37 23.438794894134137 25.116178204152583 25.66732512203235 25.777701779680935 38-39 23.310140908984025 25.278062629172794 25.883583074634842 25.528213387208343 40-41 23.676043552206323 25.360966694753014 25.525378040403112 25.437611712637555 42-43 23.461427428858087 25.000360198913935 25.744047384298586 25.794164987929392 44-45 23.344300230739705 25.14123151514973 25.953781702390106 25.56068655172046 46-47 23.59408693969594 25.346268764663254 25.589334480416966 25.47030981522384 48-49 23.44133768950804 25.201115214670093 25.584306535382467 25.773240560439397 50-51 23.507791520346697 25.14982377403673 25.75183420819665 25.59055049741993 52-53 23.802211172951115 25.276180922414927 25.436716969188865 25.484890935445097 54-55 23.562151040755342 24.993285288236137 25.564731320281943 25.879832350726577 56-57 23.472767510319244 25.11044696938901 25.771853522830913 25.644931997460834 58-59 23.694648374021046 25.239758193584507 25.56538051093052 25.500212921463927 60-61 23.631185840307754 24.971920459663615 25.50292206537318 25.893971634655454 62-63 23.601023510179225 25.073033304575265 25.723005918236463 25.602937267009047 64-65 23.906434643037265 25.131132548292822 25.438036722057056 25.524396086612853 66-67 23.753386334520705 25.005728585935707 25.40321908400371 25.837665995539883 68-69 23.595515249632186 25.15417815057321 25.74110376767498 25.509202832119627 70-71 23.90732727657855 25.171596483068793 25.441851346783395 25.47922489356927 72-73 23.78513274846377 24.831661633809606 25.423813406083877 25.959392211642747 74-75 23.688058773519863 24.89523358010898 25.771708481389606 25.64499916498156 76-77 23.907224823562917 25.080604519700334 25.469235144138487 25.542935512598262 78-79 23.77875397580255 24.887388192713004 25.50156318818034 25.8322946433041 80-81 23.689600018172722 25.02073614879421 25.65405762288383 25.635606210149238 82-83 23.987087496508487 25.085426419069222 25.32669847450269 25.6007876099196 84-85 23.888730489884825 24.767690711845823 25.357820195056192 25.98575860321316 86-87 23.765060261553987 24.828493263171524 25.694962287527023 25.71148418774747 88-89 24.021334891478297 25.004955965262702 25.343418157373694 25.630290985885306 90-91 24.005337553006648 24.7637555415026 25.38192499177313 25.848981913717623 92-93 24.049750219817394 24.848728140585955 25.58026086346996 25.52126077612669 94-95 24.251297507686186 24.879244162825877 25.278476083500102 25.590982245987842 96-97 24.059059869927065 24.59657392892752 25.34169503707096 26.00267116407446 98-99 23.95197492789403 24.766870955952918 25.60122836492324 25.67992575122981 100-101 24.152323310535103 24.93099607397938 25.35825851849503 25.55842209699049 102-103 24.114358508090284 24.71853416546614 25.330765638496743 25.836341687946835 104-105 24.084292090739773 24.884478744167804 25.519576813727756 25.511652351364667 106-107 24.325425303815482 24.89520684776898 25.189073579947358 25.590294268468185 108-109 24.29091611219479 24.76457994259602 25.15532539089268 25.78917855431651 110-111 24.153229249791668 24.93977666096106 25.478489756616312 25.428504332630965 112-113 24.358685219581318 25.020295744984377 25.16897959372543 25.45203944170887 114-115 24.30947970989314 24.827490868431802 24.984666789781055 25.878362631894003 116-117 24.28697545208653 24.9038532474989 25.28297688087257 25.526194419541998 118-119 24.497123792500805 25.06556099875672 24.974754703638837 25.462560505103642 120-121 24.45366238746746 24.98131326311625 24.868943071851877 25.696081277564414 122-123 24.44457601885922 25.105760239071877 25.045452767192227 25.404210974876673 124-125 24.731016791653012 25.270040127158378 24.679637917493448 25.319305163695166 126 24.518549819051294 24.95837527705996 24.87979520383915 25.643279700049597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11.0 1 9.0 2 6.5 3 4.5 4 5.5 5 6.0 6 5.0 7 6.5 8 5.5 9 4.5 10 5.0 11 3.5 12 5.0 13 9.0 14 8.0 15 8.0 16 9.0 17 13.0 18 19.5 19 26.0 20 46.5 21 103.0 22 189.5 23 286.0 24 475.0 25 784.5 26 1280.0 27 2095.5 28 3325.5 29 5313.0 30 8887.0 31 14245.0 32 21927.5 33 33248.0 34 50269.0 35 73554.0 36 103840.0 37 143277.5 38 191199.0 39 246843.0 40 309379.0 41 371750.0 42 429281.0 43 469816.0 44 492528.5 45 507381.0 46 508966.5 47 493240.0 48 463672.5 49 429681.0 50 396529.5 51 357551.0 52 315310.0 53 277817.0 54 243230.0 55 218336.5 56 202143.5 57 185761.0 58 171019.5 59 163680.5 60 159310.0 61 148434.0 62 134757.0 63 127449.0 64 125078.0 65 119200.5 66 109355.0 67 102139.0 68 95503.0 69 84838.0 70 74128.0 71 64281.0 72 54760.5 73 47290.5 74 40023.5 75 32196.5 76 25052.5 77 18756.5 78 13506.5 79 9339.0 80 6203.5 81 3993.5 82 2391.5 83 1312.5 84 609.0 85 243.5 86 117.0 87 53.5 88 17.0 89 5.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10223191806470909 2 0.026123247039680834 3 0.0 4 0.0 5 0.0 6 7.46678961279122E-4 7 0.0 8 0.0 9 0.0 10-11 9.149446426941354E-4 12-13 8.939114325172588E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6291512721095845E-5 24-25 0.06129603275796285 26-27 0.0035756457300690353 28-29 4.679889264355061E-4 30-31 6.309963053063004E-5 32-33 5.25830254421917E-6 34-35 0.16342804307433179 36-37 0.028247601267545375 38-39 0.04525820999809439 40-41 0.0 42-43 0.0014407748971160524 44-45 5.784132798641086E-5 46-47 0.10411964867808378 48-49 0.03509916948266295 50-51 0.07564068209859275 52-53 0.04917564539353767 54-55 0.01748385595952874 56-57 0.009764667824614997 58-59 0.01835673418186912 60-61 0.005410793318001525 62-63 0.013613745286983428 64-65 0.014796863359432743 66-67 0.017105258176344958 68-69 0.013292988831786059 70-71 0.00746678961279122 72-73 0.016626752644821014 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5774907632657508E-4 82-83 4.206642035375336E-5 84-85 0.004574723213470678 86-87 0.00189824721846312 88-89 3.0498154756471185E-4 90-91 0.00310239850108931 92-93 0.0032759224850485427 94-95 0.006725368954056317 96-97 0.001929797033728435 98-99 0.0020717712024223528 100-101 0.008397509163118013 102-103 0.0011936346775377514 104-105 0.0025292435237694202 106-107 4.206642035375335E-4 108-109 2.5239852212252016E-4 110-111 0.0 112-113 5.784132798641086E-5 114-115 0.0 116-117 5.25830254421917E-6 118-119 7.361623561906837E-4 120-121 2.3662361448986263E-4 122-123 0.0 124-125 0.0 126 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 126 9508772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.006611701023814 #Duplication Level Percentage of deduplicated Percentage of total 1 71.74778653742842 35.878637017851275 2 14.261959846127729 14.263845762418054 3 5.3948028518874525 8.093274342537352 4 2.6952914769356684 5.391295772328038 5 1.5017228308852206 3.754803524331974 6 0.9590809688153727 2.8776233758435246 7 0.6684716738606812 2.3399602399519157 8 0.48743255406248076 1.9499880369152622 9 0.3536846006214871 1.591791163911935 >10 1.7874409228770558 15.840632239869972 >50 0.096487559447262 3.291785061061185 >100 0.0436272710805965 3.895758934969837 >500 0.001937455193763438 0.6315167500794746 >1k 2.7345077687194205E-4 0.19908777793011959 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.051660508843834E-5 0.0 0.0 1.051660508843834E-5 0.0 2 1.051660508843834E-5 0.0 0.0 1.051660508843834E-5 0.0 3 1.051660508843834E-5 0.0 0.0 2.103321017687668E-5 0.0 4 2.103321017687668E-5 0.0 0.0 6.309963053063003E-5 2.103321017687668E-5 5 3.1549815265315014E-5 0.0 0.0 7.361623561906837E-5 2.103321017687668E-5 6 4.206642035375336E-5 0.0 0.0 7.361623561906837E-5 2.103321017687668E-5 7 4.206642035375336E-5 0.0 0.0 7.361623561906837E-5 2.103321017687668E-5 8 4.206642035375336E-5 0.0 0.0 8.413284070750672E-5 2.103321017687668E-5 9 4.206642035375336E-5 0.0 1.051660508843834E-5 8.413284070750672E-5 2.103321017687668E-5 10-11 4.206642035375336E-5 0.0 1.051660508843834E-5 9.464944579594505E-5 6.309963053063003E-5 12-13 4.206642035375336E-5 0.0 2.103321017687668E-5 9.464944579594505E-5 1.367158661496984E-4 14-15 4.206642035375336E-5 1.051660508843834E-5 2.103321017687668E-5 9.464944579594505E-5 2.576568246667393E-4 16-17 5.2583025442191696E-5 2.103321017687668E-5 3.1549815265315014E-5 9.464944579594505E-5 3.154981526531502E-4 18-19 5.784132798641086E-5 2.103321017687668E-5 3.1549815265315014E-5 1.1042435342860256E-4 3.4704796791846517E-4 20-21 6.309963053063003E-5 2.103321017687668E-5 3.1549815265315014E-5 1.4723247123813674E-4 3.73339480639561E-4 22-23 8.413284070750672E-5 2.103321017687668E-5 3.1549815265315014E-5 1.7352398395923259E-4 4.311808086259719E-4 24-25 9.464944579594505E-5 2.103321017687668E-5 3.1549815265315014E-5 1.892988915918901E-4 5.205719518776978E-4 26-27 9.464944579594505E-5 2.103321017687668E-5 4.732472289797253E-5 1.892988915918901E-4 5.468634645987937E-4 28-29 9.990774834016421E-5 2.103321017687668E-5 5.2583025442191696E-5 1.9981549668032843E-4 5.468634645987937E-4 30-31 1.0516605088438339E-4 2.103321017687668E-5 5.2583025442191696E-5 2.1559040431298596E-4 5.468634645987937E-4 32-33 1.3145756360547923E-4 2.103321017687668E-5 5.2583025442191696E-5 2.523985221225201E-4 5.626383722314511E-4 34-35 1.6300737887079425E-4 2.6291512721095848E-5 5.2583025442191696E-5 2.523985221225201E-4 5.784132798641086E-4 36-37 2.1033210176876679E-4 4.732472289797252E-5 7.361623561906837E-5 2.523985221225201E-4 6.099630951294237E-4 38-39 2.6291512721095847E-4 6.309963053063003E-5 7.361623561906837E-5 2.6817342975517765E-4 6.309963053063003E-4 40-41 3.73339480639561E-4 6.309963053063003E-5 7.361623561906837E-5 2.734317322993968E-4 6.309963053063003E-4 42-43 4.627306238912869E-4 6.309963053063003E-5 7.361623561906837E-5 2.9972324502049267E-4 6.362546078505194E-4 44-45 5.626383722314511E-4 6.309963053063003E-5 7.361623561906837E-5 3.1023985010893097E-4 6.415129103947387E-4 46-47 6.415129103947387E-4 6.309963053063003E-5 7.887453816328755E-5 3.1549815265315015E-4 6.52029515483177E-4 48-49 8.150368943539712E-4 6.309963053063003E-5 8.413284070750672E-5 3.1549815265315015E-4 6.83579330748492E-4 50-51 0.001051660508843834 6.309963053063003E-5 1.0516605088438339E-4 3.1549815265315015E-4 7.098708434695878E-4 52-53 0.0014039667793065182 6.835793307484919E-5 1.2094095851704089E-4 3.2075645519736933E-4 7.151291460138071E-4 54-55 0.0017299815370481067 8.413284070750672E-5 1.367158661496984E-4 3.365313628300268E-4 7.203874485580263E-4 56-57 0.0020244464795243803 8.413284070750672E-5 1.367158661496984E-4 3.4704796791846517E-4 7.940036841770946E-4 58-59 0.002518726918680982 9.464944579594505E-5 1.367158661496984E-4 3.5230627046268435E-4 8.308118019866288E-4 60-61 0.003128690013810406 9.464944579594505E-5 1.367158661496984E-4 3.6808117809534183E-4 8.781365248846013E-4 62-63 0.003943726908164377 9.464944579594505E-5 1.4197416869391757E-4 3.9963099336065685E-4 8.833948274288205E-4 64-65 0.005200461216232758 9.464944579594505E-5 1.6826568141501343E-4 4.2066420353753357E-4 9.044280376056972E-4 66-67 0.006562361575185523 9.990774834016421E-5 1.6826568141501343E-4 4.2066420353753357E-4 9.149446426941354E-4 68-69 0.008213468574070341 1.0516605088438339E-4 1.7352398395923259E-4 4.2066420353753357E-4 9.149446426941354E-4 70-71 0.010500830180805682 1.0516605088438339E-4 1.9981549668032843E-4 4.469557162586294E-4 9.202029452383546E-4 72-73 0.01398708476762299 1.0516605088438339E-4 1.9981549668032843E-4 4.7324722897972525E-4 9.727859706805463E-4 74-75 0.018425092114943968 1.0516605088438339E-4 2.1033210176876679E-4 4.942804391566019E-4 0.0010043357859458614 76-77 0.023888468458387685 1.0516605088438339E-4 2.1033210176876679E-4 5.047970442450402E-4 0.0010358856012111764 78-79 0.030850461026933867 1.0516605088438339E-4 2.1033210176876679E-4 5.153136493334786E-4 0.0010411439037553956 80-81 0.040210239555643984 1.0516605088438339E-4 2.1033210176876679E-4 5.363468595103553E-4 0.0010674354164764914 82-83 0.05185737969108945 1.0516605088438339E-4 2.3136531194564345E-4 5.57380069687232E-4 0.0011095018368302448 84-85 0.06828957514177436 1.0516605088438339E-4 2.3136531194564345E-4 5.57380069687232E-4 0.001114760139374464 86-87 0.08913348642705914 1.0516605088438339E-4 2.3136531194564345E-4 5.784132798641086E-4 0.0011252767444629023 88-89 0.11504114306242699 1.0516605088438339E-4 2.3136531194564345E-4 5.784132798641086E-4 0.001146309954639779 90-91 0.146927489690572 1.0516605088438339E-4 2.3662361448986263E-4 5.836715824083277E-4 0.001177859769905094 92-93 0.18875728642983552 1.0516605088438339E-4 2.523985221225201E-4 5.88929884952547E-4 0.0012619926106126006 94-95 0.24055682479293858 1.0516605088438339E-4 2.734317322993968E-4 5.88929884952547E-4 0.0013198339385990115 96-97 0.3065380051178007 1.1042435342860256E-4 2.734317322993968E-4 5.994464900409853E-4 0.0015880073683541892 98-99 0.3875737056267623 1.1568265597282172E-4 2.734317322993968E-4 5.994464900409853E-4 0.001677398511605915 100-101 0.48340626949515664 1.2619926106126006E-4 2.734317322993968E-4 6.099630951294237E-4 0.0017036900243270108 102-103 0.5993991653180873 1.4723247123813674E-4 2.734317322993968E-4 6.204797002178619E-4 0.0017299815370481067 104-105 0.7407213044965217 1.4723247123813674E-4 2.734317322993968E-4 6.257380027620812E-4 0.0017404981421365452 106-107 0.910780067079114 1.4723247123813674E-4 2.944649424762735E-4 6.309963053063003E-4 0.0017562730497692027 108-109 1.1162114308766684 1.4723247123813674E-4 3.0498154756471185E-4 6.309963053063003E-4 0.001766789654857641 110-111 1.3578882741115255 1.4723247123813674E-4 3.1549815265315015E-4 6.309963053063003E-4 0.0018088560752113944 112-113 1.6438768328865179 1.4723247123813674E-4 3.1549815265315015E-4 6.309963053063003E-4 0.0018772140082862435 114 1.8959230487385752 1.4723247123813674E-4 3.1549815265315015E-4 6.309963053063003E-4 0.0018824723108304625 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGATT 3775 0.0 12.247362 1 CCCGGTT 3010 0.0 11.370437 1 GTCCGCT 2230 0.0 11.308714 1 CGATTTT 4195 0.0 11.292922 3 GCGATTT 3450 0.0 10.952886 2 GTCGGTT 2850 0.0 10.323335 1 GTCGGAT 3415 0.0 9.846142 1 ACGTCTG 9945 0.0 9.828706 120 GATCGGA 33535 0.0 9.638377 120 TCGGAAG 29130 0.0 9.572513 120 ATCGGAA 30830 0.0 9.511497 120 CACACGT 14165 0.0 9.3983135 120 CCCCGCT 3345 0.0 9.334177 1 GGGGGGT 2410 0.0 9.330892 16-17 CACGTCT 11705 0.0 9.324241 120 TTATGGG 2355 0.0 9.294225 12-13 GTCCGTA 1895 0.0 9.188774 1 GATTTTA 4725 0.0 9.010891 4 CGGAAGA 29610 0.0 8.789513 120 CCCCACT 5065 0.0 8.772452 1 >>END_MODULE SRR7692619 read2 length is 126 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7692619_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9508772 Sequences flagged as poor quality 0 Sequence length 126 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62395133672361 33.0 33.0 34.0 32.0 34.0 2 32.7036463804159 33.0 33.0 34.0 31.0 34.0 3 32.71931464967295 33.0 33.0 34.0 31.0 34.0 4 32.62418827583625 33.0 33.0 34.0 31.0 34.0 5 32.701810391499556 33.0 33.0 34.0 32.0 34.0 6 36.793732460931864 38.0 38.0 38.0 35.0 38.0 7 36.80979226339637 38.0 38.0 38.0 35.0 38.0 8 36.81285091282029 38.0 38.0 38.0 35.0 38.0 9 36.81502553642048 38.0 38.0 38.0 35.0 38.0 10-11 36.827891919166845 38.0 38.0 38.0 35.0 38.0 12-13 36.80637599681641 38.0 38.0 38.0 35.0 38.0 14-15 36.787663275552305 38.0 38.0 38.0 35.0 38.0 16-17 36.81464809546385 38.0 38.0 38.0 35.0 38.0 18-19 36.78103807726171 38.0 38.0 38.0 35.0 38.0 20-21 36.83459210085172 38.0 38.0 38.0 35.0 38.0 22-23 36.843413061118724 38.0 38.0 38.0 35.0 38.0 24-25 36.84325631111988 38.0 38.0 38.0 36.0 38.0 26-27 36.85105889593315 38.0 38.0 38.0 36.0 38.0 28-29 36.8641073211136 38.0 38.0 38.0 36.0 38.0 30-31 36.8802355340942 38.0 38.0 38.0 36.0 38.0 32-33 36.90718380880307 38.0 38.0 38.0 36.0 38.0 34-35 36.920148101142814 38.0 38.0 38.0 36.0 38.0 36-37 36.92198845445027 38.0 38.0 38.0 36.0 38.0 38-39 36.927338672122964 38.0 38.0 38.0 36.0 38.0 40-41 36.93514204568161 38.0 38.0 38.0 36.0 38.0 42-43 36.919167795799495 38.0 38.0 38.0 36.0 38.0 44-45 36.922354432307344 38.0 38.0 38.0 36.0 38.0 46-47 36.930308876898096 38.0 38.0 38.0 36.0 38.0 48-49 36.92248368138388 38.0 38.0 38.0 36.0 38.0 50-51 36.9305089553099 38.0 38.0 38.0 36.0 38.0 52-53 36.93015097007269 38.0 38.0 38.0 36.0 38.0 54-55 36.92139863065388 38.0 38.0 38.0 36.0 38.0 56-57 36.92091302641393 38.0 38.0 38.0 36.0 38.0 58-59 36.91785243141806 38.0 38.0 38.0 36.0 38.0 60-61 36.91109982445683 38.0 38.0 38.0 36.0 38.0 62-63 36.89894867602251 38.0 38.0 38.0 36.0 38.0 64-65 36.89381304967665 38.0 38.0 38.0 36.0 38.0 66-67 36.86746453695598 38.0 38.0 38.0 36.0 38.0 68-69 36.85998964955727 38.0 38.0 38.0 35.0 38.0 70-71 36.829714394245656 38.0 38.0 38.0 35.0 38.0 72-73 36.84142573825516 38.0 38.0 38.0 35.0 38.0 74-75 36.831787480023706 38.0 38.0 38.0 35.0 38.0 76-77 36.81757996721343 38.0 38.0 38.0 35.0 38.0 78-79 36.79346896739138 38.0 38.0 38.0 35.0 38.0 80-81 36.76976564376557 38.0 38.0 38.0 35.0 38.0 82-83 36.74261818455632 38.0 38.0 38.0 35.0 38.0 84-85 36.71024355195392 38.0 38.0 38.0 35.0 38.0 86-87 36.68625959272133 38.0 38.0 38.0 35.0 38.0 88-89 36.66740768418887 38.0 38.0 38.0 35.0 38.0 90-91 36.60667912744148 38.0 38.0 38.0 34.0 38.0 92-93 36.589107878493664 38.0 38.0 38.0 34.0 38.0 94-95 36.57111827899544 38.0 38.0 38.0 34.0 38.0 96-97 36.52937797856548 38.0 38.0 38.0 34.0 38.0 98-99 36.50801365307739 38.0 38.0 38.0 34.0 38.0 100-101 36.444986587121875 38.0 38.0 38.0 34.0 38.0 102-103 36.40710241027969 38.0 38.0 38.0 34.0 38.0 104-105 36.37079924726348 38.0 38.0 38.0 34.0 38.0 106-107 36.339307483658246 38.0 38.0 38.0 34.0 38.0 108-109 36.26617643161494 38.0 38.0 38.0 34.0 38.0 110-111 36.227113816589565 38.0 38.0 38.0 33.5 38.0 112-113 36.184792315979394 38.0 38.0 38.0 33.0 38.0 114-115 36.126797603307764 38.0 37.0 38.0 33.0 38.0 116-117 35.97305977049402 38.0 37.0 38.0 32.5 38.0 118-119 35.91412766022784 38.0 37.0 38.0 32.0 38.0 120-121 35.86994351110742 38.0 37.0 38.0 31.0 38.0 122-123 35.651327952757725 38.0 36.0 38.0 31.0 38.0 124-125 35.54827437233746 38.0 36.0 38.0 31.0 38.0 126 31.10332133318582 35.0 28.0 38.0 14.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13531020570329133 1101 2 0.11266140728501028 1101 3 0.16885201958256602 1101 4 0.19092873764228102 1101 5 0.170373348615378 1101 6 0.2715251798224756 1101 7 0.241442860728597 1101 8 0.20189469064329302 1101 9 0.2211117643413658 1101 10-11 0.18362649388881636 1101 12-13 0.20326488872983361 1101 14-15 0.20272157645152333 1101 16-17 0.22536684495768355 1101 18-19 0.22411564615084245 1101 20-21 0.20314632945871836 1101 22-23 0.20560985107379537 1101 24-25 0.1540180138169731 1101 26-27 0.0898780494689646 1101 28-29 0.06515157336904309 1101 30-31 0.07221356816295099 1101 32-33 0.07975299457603313 1101 34-35 0.06915462848554199 1101 36-37 0.05558222416313896 1101 38-39 0.009354952242105696 1101 40-41 0.05450652817528123 1101 42-43 0.071664121129551 1101 44-45 0.05157812665731143 1101 46-47 0.034231722777533946 1101 48-49 0.04136331074044364 1101 50-51 0.04795516630692731 1101 52-53 0.025677158461327565 1101 54-55 0.04228453223890938 1101 56-57 -0.03398707577568416 1101 58-59 -0.036884088476384136 1101 60-61 -0.038278262692593046 1101 62-63 -0.014857807173690674 1101 64-65 -0.009304402574194626 1101 66-67 0.01068847557903041 1101 68-69 -0.05012002813536043 1101 70-71 -0.008699278730702531 1101 72-73 -0.07169261788182979 1101 74-75 -0.08794198672658382 1101 76-77 -0.04346547552663793 1101 78-79 -0.05163192038576625 1101 80-81 -0.0865925096888489 1101 82-83 -0.12286048832188357 1101 84-85 -0.06411224587203179 1101 86-87 -0.08069264165042256 1101 88-89 -0.07647607115929844 1101 90-91 -0.0615609230176517 1101 92-93 -0.059793263528092666 1101 94-95 -0.13164233089023725 1101 96-97 -0.1012435638886231 1101 98-99 -0.15416082382238017 1101 100-101 -0.10115226345916284 1101 102-103 -0.09071656734701605 1101 104-105 -0.13413214831835774 1101 106-107 -0.1687257119120602 1101 108-109 -0.17961029923538518 1101 110-111 -0.22696000162522978 1101 112-113 -0.18550511941460712 1101 114-115 -0.23222595062429718 1101 116-117 -0.1489334334141077 1101 118-119 -0.2548945815075143 1101 120-121 -0.34775035140059884 1101 122-123 -0.275176899287743 1101 124-125 -0.36824441264482743 1101 126 -0.39908493601941686 1102 1 0.08923405145139185 1102 2 0.0861379371102231 1102 3 0.11706522685715726 1102 4 0.1651597072275166 1102 5 0.0801177857555686 1102 6 0.13308499891302006 1102 7 0.12074571670758871 1102 8 0.13286089254051348 1102 9 0.12369187704494067 1102 10-11 0.11356778053024641 1102 12-13 0.1511637498636773 1102 14-15 0.18616937382372356 1102 16-17 0.17795054964080492 1102 18-19 0.15805831342064636 1102 20-21 0.1502280913714813 1102 22-23 0.15732006753200523 1102 24-25 0.11735255838610925 1102 26-27 0.09912917304805546 1102 28-29 0.12507181450647664 1102 30-31 0.1010263867819603 1102 32-33 0.07855124904313726 1102 34-35 0.04437880015080964 1102 36-37 0.05544180426580425 1102 38-39 0.06014943947263873 1102 40-41 0.02909889488605444 1102 42-43 0.04003422629616438 1102 44-45 0.04729032936180033 1102 46-47 0.031245738941102275 1102 48-49 0.03339531330455969 1102 50-51 0.011888240679404305 1102 52-53 0.03428910704312926 1102 54-55 -0.017776286685062814 1102 56-57 -0.014973216824657243 1102 58-59 -0.008376469701687483 1102 60-61 -0.033777337518202444 1102 62-63 -0.008758490574585664 1102 64-65 -0.06234942569763291 1102 66-67 -0.025156452179331268 1102 68-69 -0.029690692410234476 1102 70-71 -0.015529503958532587 1102 72-73 -0.0557869377385245 1102 74-75 -0.027989835051890566 1102 76-77 -0.012666951971205265 1102 78-79 -0.042603522093990875 1102 80-81 -0.08122329535571282 1102 82-83 -0.10128586373449622 1102 84-85 -0.043998530679125736 1102 86-87 -0.10192743226733825 1102 88-89 -0.12358009545078374 1102 90-91 -0.1289851703118572 1102 92-93 -0.11589508379896785 1102 94-95 -0.12987289470020613 1102 96-97 -0.14558492735444162 1102 98-99 -0.17197261582319356 1102 100-101 -0.16635699445147623 1102 102-103 -0.08563295767936552 1102 104-105 -0.13899533900894312 1102 106-107 -0.23616125392373277 1102 108-109 -0.16503175685279103 1102 110-111 -0.21774324150506175 1102 112-113 -0.16701775331453206 1102 114-115 -0.24269372898888975 1102 116-117 -0.1226673734028978 1102 118-119 -0.22753065014736507 1102 120-121 -0.3342562541451386 1102 122-123 -0.2670622735252124 1102 124-125 -0.35209182760936386 1102 126 -0.48523049611278424 1103 1 0.1039917186781878 1103 2 0.1349998759221691 1103 3 0.15225267696187927 1103 4 0.1948708163039612 1103 5 0.16751010260075105 1103 6 0.16140432612198907 1103 7 0.1713104304507027 1103 8 0.18850596002256026 1103 9 0.14568390818480026 1103 10-11 0.16647568459266893 1103 12-13 0.15155397907588508 1103 14-15 0.15836169045952175 1103 16-17 0.160362474559399 1103 18-19 0.12202482426190642 1103 20-21 0.09730039818624192 1103 22-23 0.11286999928969266 1103 24-25 0.08675765274586666 1103 26-27 0.08892998545584163 1103 28-29 0.11312436772549717 1103 30-31 0.1187332136697492 1103 32-33 0.04740470026730037 1103 34-35 0.032258133112392784 1103 36-37 0.04216499840820376 1103 38-39 -0.002142594086521399 1103 40-41 0.0918037063330388 1103 42-43 0.06166998494752107 1103 44-45 0.02110302694151045 1103 46-47 0.0488611933379417 1103 48-49 0.06432843200955318 1103 50-51 0.031035098934815153 1103 52-53 0.0374242947003367 1103 54-55 0.011935806726697251 1103 56-57 0.007643559995386795 1103 58-59 -0.005326207992524701 1103 60-61 -0.030896512349791294 1103 62-63 0.01429958140861487 1103 64-65 -0.03323104579931879 1103 66-67 -0.001381994604422232 1103 68-69 -0.057826142367204625 1103 70-71 -0.03395825830277488 1103 72-73 -0.050935217214835404 1103 74-75 -0.06388217166342969 1103 76-77 -0.027588639049575647 1103 78-79 -0.0016964635812968254 1103 80-81 -0.02506986704323566 1103 82-83 -0.002138517749465052 1103 84-85 -0.031231126885273852 1103 86-87 -0.09436673559098807 1103 88-89 -0.07748479890992144 1103 90-91 -0.11497093229402111 1103 92-93 -0.05933071540393087 1103 94-95 -0.11016002087554 1103 96-97 -0.19505380277397677 1103 98-99 -0.10874575957787158 1103 100-101 -0.08246645904839767 1103 102-103 -0.06469639004548355 1103 104-105 -0.08989300616609341 1103 106-107 -0.1291343321364664 1103 108-109 -0.21454794295557633 1103 110-111 -0.17560105837586093 1103 112-113 -0.07231156329739008 1103 114-115 -0.14088263506780407 1103 116-117 -0.0746915241439794 1103 118-119 -0.11365865484231819 1103 120-121 -0.1400666899612517 1103 122-123 -0.14972044500252224 1103 124-125 -0.20200640892466737 1103 126 0.04593874927345354 1104 1 0.11850264771147323 1104 2 0.1192052518545097 1104 3 0.13675001561463773 1104 4 0.16163868125872227 1104 5 0.13625365923851973 1104 6 0.1978050271598022 1104 7 0.20237602756014894 1104 8 0.245665469579734 1104 9 0.26083142776989376 1104 10-11 0.18328778033134796 1104 12-13 0.20859362164041784 1104 14-15 0.20766510504603986 1104 16-17 0.2347995360698505 1104 18-19 0.24099932600250185 1104 20-21 0.17736923102264512 1104 22-23 0.15536516642709586 1104 24-25 0.13639257661463233 1104 26-27 0.14187488836766704 1104 28-29 0.1534833727776359 1104 30-31 0.11152322769923018 1104 32-33 0.11130035298193519 1104 34-35 0.11607454815383278 1104 36-37 0.08783285665368368 1104 38-39 0.109336504600968 1104 40-41 0.11163606845735785 1104 42-43 0.10702760723367533 1104 44-45 0.023052126970860343 1104 46-47 0.011362188820356778 1104 48-49 0.06866761032473079 1104 50-51 0.02919942253587493 1104 52-53 0.04507144063860835 1104 54-55 0.04607031931123373 1104 56-57 0.033751006912993375 1104 58-59 -0.004077661669597887 1104 60-61 -0.008287027011469661 1104 62-63 -0.009983541700641752 1104 64-65 0.008365971189881805 1104 66-67 0.023073701518711687 1104 68-69 0.009772739037835265 1104 70-71 0.009472560032861566 1104 72-73 -0.036668385925374025 1104 74-75 -0.017566465732762993 1104 76-77 -0.00780216457373939 1104 78-79 0.0016283454224463867 1104 80-81 -0.005078330007478371 1104 82-83 -0.019308134862377813 1104 84-85 0.04281224178359366 1104 86-87 -0.017121800005519106 1104 88-89 -0.02671734333025455 1104 90-91 -0.02467679042168669 1104 92-93 -0.04500937122447368 1104 94-95 -0.06221595116885936 1104 96-97 -0.07478282155014426 1104 98-99 -0.055047324203492565 1104 100-101 -0.03338569842345862 1104 102-103 -0.036628320048613716 1104 104-105 -0.009234759098809775 1104 106-107 -0.051599104331906176 1104 108-109 -0.1370868308626143 1104 110-111 -0.07382525410788787 1104 112-113 -0.10734364646844341 1104 114-115 -0.10991849534462261 1104 116-117 -0.054774375554735855 1104 118-119 -0.032452721985286814 1104 120-121 -0.10270371197967876 1104 122-123 0.018684327149280477 1104 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0.10288052799019454 2316 92-93 0.02404318354604129 2316 94-95 -0.0015297756134273754 2316 96-97 0.05530974375576392 2316 98-99 0.039175739949413924 2316 100-101 -0.014457883030985386 2316 102-103 0.033316580970300436 2316 104-105 0.038871587552335995 2316 106-107 0.1059433227123634 2316 108-109 0.0395197699640093 2316 110-111 0.05548836159353243 2316 112-113 -0.03472054019854198 2316 114-115 0.0227609807837581 2316 116-117 0.048447206521842645 2316 118-119 0.02425711153591692 2316 120-121 0.09935446473489407 2316 122-123 -9.485335650154525E-4 2316 124-125 -0.019514098612205544 2316 126 -0.6223207533899036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 19.0 7 69.0 8 278.0 9 1539.0 10 3085.0 11 741.0 12 299.0 13 289.0 14 312.0 15 781.0 16 2740.0 17 5410.0 18 7448.0 19 8200.0 20 8693.0 21 10648.0 22 14382.0 23 19727.0 24 26738.0 25 33964.0 26 43013.0 27 53312.0 28 65017.0 29 80012.0 30 98753.0 31 122243.0 32 157624.0 33 210422.0 34 300062.0 35 484645.0 36 1104575.0 37 6643731.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.264308062504334 16.941573971807273 12.90653011386961 36.887587851818786 2 28.536112329627795 24.125662718532393 28.144962340034407 19.193262611805405 3 22.9319883244196 26.066937400190454 26.18656424855672 24.814510026833222 4 26.507553846786475 30.191745387517134 19.95074142888979 23.34995933680661 5 27.67266520826592 32.063842490194915 19.959238957536183 20.304253344002984 6 22.84000707558232 34.97884792104289 20.560003735503628 21.621141267871156 7 22.737736331831098 18.532363514446796 34.55483005751459 24.175070096207513 8 23.671192803808978 22.714447626209985 24.945068709203795 28.669290860777245 9 23.878105098095308 22.378792125451774 27.667319951768732 26.075782824684186 10-11 26.289665470390588 28.18221390863401 21.10319658541777 24.424924035557627 12-13 26.130080344862183 23.179388620128474 24.578577031217694 26.11195400379165 14-15 25.303507707322616 25.54966140785761 24.749613240377812 24.39721764444197 16-17 26.18647884112646 24.98913098576648 23.957720954386737 24.86666921872032 18-19 25.64417335986688 25.398624998586822 24.360829943680628 24.596371697865667 20-21 25.86259325870796 25.567347743194873 24.19966816692488 24.370390831172287 22-23 25.85403336417506 25.353326752736056 24.17232653420077 24.62031334888811 24-25 25.427315777438537 25.384686537231342 24.60432730459134 24.58367038073878 26-27 25.5743008227392 25.684076927801726 24.442096907294957 24.299525342164124 28-29 25.877352805423026 25.122584400287963 24.34568271893271 24.654380075356304 30-31 25.41077488691504 25.466003620129257 24.596274749843154 24.52694674311255 32-33 25.52503341602565 25.795861370358608 24.453526015661403 24.225579197954335 34-35 25.907268239804203 25.311734242197055 24.256791611570435 24.52420590642831 36-37 25.48935132858141 25.37453552121935 24.64573197392877 24.490381176270475 38-39 25.57571160083736 25.634302831456875 24.507122915854985 24.28286265185078 40-41 25.861435599689276 25.157871967765637 24.327219217676692 24.653473214868395 42-43 25.455953162695394 25.360503449868194 24.758421860889413 24.425121526547002 44-45 25.600106982508557 25.63402582684485 24.55783799759984 24.208029193046748 46-47 25.981242953529055 25.332070288557556 24.27500622873559 24.411680529177797 48-49 25.561300218825128 25.380757251046088 24.73756010007254 24.320382430056245 50-51 25.752073665097996 25.557994043558256 24.665417258817847 24.024515032525905 52-53 26.047316221344325 25.18476772384638 24.458517741411974 24.309398313397324 54-55 25.549546171531368 25.36180299556422 24.75596676708935 24.332684065815062 56-57 25.666269933624847 25.636859689028242 24.639221876927284 24.057648500419628 58-59 25.96209863050757 25.181766100766286 24.537490713867108 24.31864455485904 60-61 25.6014424513209 25.388750673445017 24.76374422584719 24.246062649386893 62-63 25.750849717624035 25.539228288169813 24.751153748411156 23.958768245794996 64-65 26.075173583712612 25.215174739691548 24.56349951249905 24.14615216409679 66-67 25.543021246784225 25.38407292083446 24.917682022178965 24.15522381020235 68-69 25.717507970264 25.650250068447377 24.728928918834622 23.903313042454005 70-71 26.03041726964303 25.261875674088895 24.60140407315731 24.106302983110766 72-73 25.545843025397595 25.326526960603736 25.081918555084254 24.04571145891442 74-75 25.681889455806743 25.529107399875283 24.974458698875363 23.814544445442607 76-77 26.003877756517706 25.20148077018111 24.675176974414185 24.11946449888699 78-79 25.582832204594197 25.39564637160619 25.016511908204 24.00500951559561 80-81 25.690727166236226 25.656950300589372 24.81162345204935 23.840699081125052 82-83 26.02436696985024 25.3127320785979 24.656309195667884 24.006591755883974 84-85 25.640299198656606 25.392033639191215 25.03643247480463 23.93123468734755 86-87 25.65754058263731 25.71221404251689 24.908889407784176 23.721355967061623 88-89 25.958713475702254 25.32990757851153 24.720858769136587 23.99052017664963 90-91 25.51561624230046 25.498073539662116 25.10306008841459 23.883250129622834 92-93 25.680831888453902 25.748335616790186 24.92595431514399 23.644878179611915 94-95 25.94701147129571 25.481270497378937 24.701976037457165 23.86974199386819 96-97 25.53162620315404 25.57342103458445 25.105188395952688 23.78976436630883 98-99 25.725849559695195 25.756616547270227 24.89802546474864 23.619508428285943 100-101 26.043753960117538 25.43375046703006 24.75234315013545 23.770152422716954 102-103 25.60680108531585 25.54981768786128 25.153175771888748 23.690205454934123 104-105 25.692614018752774 25.812542352258376 25.009083566071205 23.48576006291764 106-107 25.973180983809513 25.502405924588313 24.7902095123476 23.734203579254572 108-109 25.60660097894804 25.622283513919303 25.150428664726874 23.620686842405785 110-111 25.7987791689318 25.93818167848221 24.90773247390643 23.355306678679558 112-113 26.1251608173323 25.68080631159182 24.65567525648539 23.538357614590492 114-115 25.815933313775712 25.86032216601599 24.87839907083406 23.445345449374237 116-117 26.037118973603214 26.00642319776761 24.710705350684094 23.245752477945082 118-119 26.2424898032368 25.830774466958868 24.504494402174636 23.422241327629692 120-121 25.960494821414304 26.027308833143792 24.804375675932892 23.207820669509015 122-123 26.324772111143684 26.263474765847295 24.49728550846608 22.91446761454294 124-125 26.627121278222237 25.965215941540777 24.27638750062784 23.13127527960914 126 26.04150514427557 26.264492832140355 24.812132583680082 22.881869439903994 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18.0 1 12.5 2 7.0 3 14.0 4 20.5 5 35.5 6 69.5 7 134.5 8 256.5 9 445.0 10 642.5 11 775.0 12 863.5 13 843.0 14 714.5 15 613.0 16 549.5 17 480.0 18 425.5 19 415.0 20 438.0 21 486.0 22 556.0 23 637.5 24 790.5 25 1115.0 26 1591.0 27 2202.0 28 3142.5 29 4518.0 30 6984.0 31 10848.0 32 16605.5 33 25543.5 34 40188.0 35 61385.5 36 90432.0 37 129682.0 38 178201.0 39 235739.0 40 299519.5 41 360754.0 42 413867.0 43 451570.5 44 476584.5 45 490243.5 46 488426.0 47 473837.5 48 447716.0 49 417542.0 50 384775.5 51 354227.0 52 317184.5 53 277815.0 54 251898.0 55 231916.0 56 211597.0 57 199252.0 58 191388.5 59 181008.0 60 171348.5 61 158949.0 62 149530.5 63 142839.0 64 136974.0 65 130578.0 66 121323.0 67 114357.5 68 108377.5 69 97286.5 70 85246.0 71 74190.0 72 63082.5 73 53495.0 74 44132.0 75 33870.5 76 25109.0 77 18924.0 78 13905.5 79 9807.5 80 6397.0 81 4041.5 82 2505.0 83 1439.0 84 705.5 85 337.5 86 193.5 87 98.5 88 56.0 89 37.5 90 21.5 91 15.5 92 12.5 93 14.5 94 12.0 95 6.0 96 4.5 97 4.5 98 4.5 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01202047961608502 2 0.0041961254302868975 3 3.891143882722185E-4 4 0.0018193726802998326 5 0.001924538731184216 6 1.4723247123813674E-4 7 5.784132798641086E-4 8 7.887453816328754E-4 9 0.004385424321878787 10-11 0.003954243513252816 12-13 0.005363468595103553 14-15 0.0029656826349396116 16-17 9.464944579594505E-4 18-19 5.731549773198895E-4 20-21 7.940036841770945E-4 22-23 1.0516605088438338E-4 24-25 0.06210055304722839 26-27 0.09445488860180894 28-29 0.09991300664270843 30-31 0.08710378164499054 32-33 0.0850898517705546 34-35 0.083827859159942 36-37 0.08768745322739888 38-39 0.08627296984300391 40-41 0.087834685698637 42-43 0.08919658605758977 44-45 0.08574713958858199 46-47 0.08885479639221552 48-49 0.08690396614831022 50-51 0.09259870780369958 52-53 0.09116319120912773 54-55 0.0956222317666256 56-57 0.09129464877273323 58-59 0.09169953806863809 60-61 0.09580627235567327 62-63 0.1023686339308588 64-65 0.10862601395847961 66-67 0.10608625382962174 68-69 0.09226217644086955 70-71 0.08397509163118014 72-73 0.08278671525618661 74-75 0.08395931672354748 76-77 0.08597850490052764 78-79 0.08469022077719393 80-81 0.08609418755650046 82-83 0.09493339413333289 84-85 0.09110009157859711 86-87 0.08222407688395515 88-89 0.08817121706146702 90-91 0.0956537815818909 92-93 0.08998007313667843 94-95 0.08749289603326277 96-97 0.09247776684518254 98-99 0.10468754535285946 100-101 0.09023772996134517 102-103 0.08159833888119307 104-105 0.08516346800617368 106-107 0.08584178903437793 108-109 0.08157730567101619 110-111 0.08273413223074441 112-113 0.0891229698219707 114-115 0.09085295135901882 116-117 0.18128523851450007 118-119 0.025728874348864395 120-121 0.05024308081001416 122-123 0.019297970337284354 124-125 0.0207650367471215 126 0.03014059018346428 >>END_MODULE >>Sequence Length Distribution pass #Length Count 126 9508772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.45344490082295 #Duplication Level Percentage of deduplicated Percentage of total 1 70.54559921975701 35.59268503229549 2 15.19291557173433 15.33069857362706 3 5.77235874204211 8.737061512182159 4 2.751485935574813 5.552877761836524 5 1.5086100224852153 3.8057286323143544 6 0.9411860895558951 2.849164830649762 7 0.6519005219581011 2.3023438945801544 8 0.4591092182001283 1.853091331513606 9 0.33687321393161446 1.5296772723895669 >10 1.7100734820939913 15.242018918928391 >50 0.09006619548040114 3.0813975980213044 >100 0.03827639565490604 3.348220356849558 >500 0.0012324502349009402 0.40126757377264105 >1k 2.920785667669269E-4 0.2971692568111186 >5k 2.0862729932679053E-5 0.07659745422827757 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 2.103321017687668E-5 4 0.0 0.0 2.103321017687668E-5 0.0 2.103321017687668E-5 5 0.0 0.0 3.1549815265315014E-5 0.0 3.1549815265315014E-5 6 1.051660508843834E-5 0.0 4.206642035375336E-5 0.0 3.1549815265315014E-5 7 1.051660508843834E-5 0.0 4.206642035375336E-5 0.0 4.206642035375336E-5 8 1.051660508843834E-5 0.0 4.206642035375336E-5 0.0 4.206642035375336E-5 9 1.051660508843834E-5 1.051660508843834E-5 6.309963053063003E-5 0.0 4.206642035375336E-5 10-11 1.051660508843834E-5 1.051660508843834E-5 7.361623561906837E-5 0.0 4.206642035375336E-5 12-13 1.577490763265751E-5 1.051660508843834E-5 8.413284070750672E-5 0.0 4.732472289797253E-5 14-15 3.1549815265315014E-5 1.577490763265751E-5 8.413284070750672E-5 0.0 5.2583025442191696E-5 16-17 4.206642035375336E-5 2.103321017687668E-5 8.939114325172588E-5 0.0 5.784132798641086E-5 18-19 4.206642035375336E-5 2.103321017687668E-5 9.990774834016421E-5 0.0 7.887453816328755E-5 20-21 4.206642035375336E-5 2.103321017687668E-5 1.0516605088438339E-4 0.0 8.413284070750672E-5 22-23 5.2583025442191696E-5 2.103321017687668E-5 1.1568265597282172E-4 0.0 8.413284070750672E-5 24-25 5.2583025442191696E-5 2.103321017687668E-5 1.2619926106126006E-4 0.0 8.413284070750672E-5 26-27 5.2583025442191696E-5 2.103321017687668E-5 1.367158661496984E-4 0.0 9.990774834016421E-5 28-29 5.784132798641086E-5 2.103321017687668E-5 1.4197416869391757E-4 0.0 1.2619926106126006E-4 30-31 6.309963053063003E-5 2.103321017687668E-5 1.5249077378235592E-4 0.0 1.3145756360547923E-4 32-33 8.413284070750672E-5 2.103321017687668E-5 1.5774907632657508E-4 0.0 1.367158661496984E-4 34-35 1.1042435342860257E-4 2.103321017687668E-5 1.5774907632657508E-4 1.051660508843834E-5 1.367158661496984E-4 36-37 1.6300737887079425E-4 2.103321017687668E-5 1.5774907632657508E-4 1.051660508843834E-5 1.4723247123813674E-4 38-39 2.1033210176876679E-4 2.103321017687668E-5 1.6826568141501343E-4 1.051660508843834E-5 1.5774907632657508E-4 40-41 3.2075645519736933E-4 2.103321017687668E-5 1.6826568141501343E-4 1.051660508843834E-5 1.6300737887079425E-4 42-43 4.2066420353753357E-4 2.103321017687668E-5 1.7352398395923259E-4 1.051660508843834E-5 1.6826568141501343E-4 44-45 5.100553467892595E-4 2.103321017687668E-5 1.7878228650345176E-4 1.051660508843834E-5 1.7878228650345176E-4 46-47 5.994464900409853E-4 2.103321017687668E-5 1.7878228650345176E-4 1.051660508843834E-5 1.9455719413610925E-4 48-49 7.782287765444371E-4 2.6291512721095848E-5 1.8404058904767094E-4 1.051660508843834E-5 1.9981549668032843E-4 50-51 0.0010095940884900807 4.206642035375335E-5 1.892988915918901E-4 1.051660508843834E-5 2.1033210176876679E-4 52-53 0.0013724169640412032 5.2583025442191696E-5 1.9455719413610925E-4 1.051660508843834E-5 2.1033210176876679E-4 54-55 0.0016984317217827918 6.309963053063003E-5 2.050737992245476E-4 1.577490763265751E-5 2.2084870685720512E-4 56-57 0.0019981549668032844 7.361623561906837E-5 2.1033210176876679E-4 2.103321017687668E-5 2.3136531194564345E-4 58-59 0.002487177103415667 7.361623561906837E-5 2.1033210176876679E-4 2.103321017687668E-5 2.523985221225201E-4 60-61 0.003133948316354625 7.361623561906837E-5 2.1033210176876679E-4 2.103321017687668E-5 2.523985221225201E-4 62-63 0.00392795200053172 9.464944579594505E-5 2.1033210176876679E-4 2.103321017687668E-5 2.523985221225201E-4 64-65 0.005153136493334785 1.1042435342860256E-4 2.1559040431298596E-4 2.103321017687668E-5 2.576568246667393E-4 66-67 0.0065308117599202084 1.3145756360547923E-4 2.2084870685720512E-4 2.103321017687668E-5 2.6291512721095847E-4 68-69 0.008124077430818617 1.367158661496984E-4 2.2084870685720512E-4 2.103321017687668E-5 2.78690034843616E-4 70-71 0.010469280365540366 1.4197416869391757E-4 2.2084870685720512E-4 2.103321017687668E-5 2.8394833738783513E-4 72-73 0.013945018347269238 1.4723247123813674E-4 2.2610700940142427E-4 2.103321017687668E-5 2.8394833738783513E-4 74-75 0.018404058904767092 1.4723247123813674E-4 2.3662361448986263E-4 2.103321017687668E-5 2.8394833738783513E-4 76-77 0.023935793181285657 1.4723247123813674E-4 2.8394833738783513E-4 2.103321017687668E-5 2.944649424762735E-4 78-79 0.030808394606580115 1.5249077378235592E-4 2.8394833738783513E-4 2.103321017687668E-5 3.1023985010893097E-4 80-81 0.040020940664052096 1.6826568141501343E-4 2.8394833738783513E-4 2.103321017687668E-5 3.2075645519736933E-4 82-83 0.05159446456387849 1.6826568141501343E-4 2.892066399320543E-4 2.103321017687668E-5 3.260147577415885E-4 84-85 0.06773219507208712 1.7878228650345176E-4 3.0498154756471185E-4 2.103321017687668E-5 3.3653136283002687E-4 86-87 0.08826060820471876 1.7878228650345176E-4 3.2075645519736933E-4 2.103321017687668E-5 3.3653136283002687E-4 88-89 0.11388431650269877 1.7878228650345176E-4 3.3653136283002687E-4 3.1549815265315014E-5 3.4704796791846517E-4 90-91 0.14547093988582332 1.7878228650345176E-4 3.3653136283002687E-4 3.1549815265315014E-5 3.4704796791846517E-4 92-93 0.18695368865716835 1.7878228650345176E-4 3.3653136283002687E-4 3.6808117809534186E-5 3.5756457300690353E-4 94-95 0.238479795287972 1.7878228650345176E-4 3.5230627046268435E-4 4.206642035375336E-5 3.5756457300690353E-4 96-97 0.30397721177876597 1.8404058904767094E-4 3.680811780953419E-4 4.206642035375336E-5 3.5756457300690353E-4 98-99 0.3839139270559858 1.892988915918901E-4 3.785977831837802E-4 4.206642035375336E-5 3.5756457300690353E-4 100-101 0.47854233964175397 1.892988915918901E-4 3.785977831837802E-4 4.206642035375336E-5 3.6808117809534183E-4 102-103 0.5934625417456638 1.892988915918901E-4 3.785977831837802E-4 4.206642035375336E-5 3.8385608572799937E-4 104-105 0.7332071901608326 1.9455719413610925E-4 3.8911438827221855E-4 4.206642035375336E-5 3.9963099336065685E-4 106-107 0.9013992553402269 1.9981549668032843E-4 3.8911438827221855E-4 4.206642035375336E-5 4.154059009933144E-4 108-109 1.1041751763529508 1.9981549668032843E-4 3.8911438827221855E-4 4.206642035375336E-5 4.2066420353753357E-4 110-111 1.3418136432338477 1.9981549668032843E-4 3.8911438827221855E-4 4.206642035375336E-5 4.2066420353753357E-4 112-113 1.6234956522251243 1.9981549668032843E-4 3.8911438827221855E-4 4.206642035375336E-5 4.2066420353753357E-4 114 1.872449986181181 1.9981549668032843E-4 4.101475984490952E-4 4.206642035375336E-5 4.2066420353753357E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACACA 8070 0.0 12.92415 2 AGCCTAG 5040 0.0 12.666306 10-11 AGCACAC 8770 0.0 12.098894 1 CACACAC 10405 0.0 11.521578 3 GACCGAA 5850 0.0 11.181489 60-61 CACACAA 9385 0.0 11.049735 5 ACACACA 11585 0.0 10.969207 4 ACACAAG 10585 0.0 10.589595 6 ACTCGAC 6205 0.0 10.492783 56-57 CGTGTAG 9205 0.0 10.362515 120 GCCTAGA 5280 0.0 10.103799 12-13 GCACTCG 6500 0.0 10.06191 54-55 CCTAGAA 6350 0.0 10.053204 12-13 GTGTAGG 8820 0.0 9.930614 120 TTAATCT 6635 0.0 9.8583 28-29 CGACCGA 5510 0.0 9.638749 60-61 CACAAGC 11570 0.0 9.480879 7 AGCGGCT 10395 0.0 9.349371 120 TCGGAAG 29980 0.0 9.2449 120 CCTAAGC 5845 0.0 9.237888 40-41 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7692619 SRR7692619_1.fastq SRR7692619_2.fastq Input file: SRR7692619_1.fastq Paired file: SRR7692619_2.fastq trimmed: SRR7692619-trimmed-pair1.fastq, SRR7692619-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Apr 10 11:04:25 2025 >> started Thu Apr 10 11:04:35 2025 >> done (9.689s) 9508772 read pairs processed; of these: 4 ( 0.00%) short read pairs filtered out after trimming by size control 63 ( 0.00%) empty read pairs filtered out after trimming by size control 9508705 (100.00%) read pairs available; of these: 530232 ( 5.58%) trimmed read pairs available after processing 8978473 (94.42%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 0 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 1 0.00% 29 0 0.00% 30 0 0.00% 31 2 0.00% 32 1 0.00% 33 0 0.00% 34 5 0.00% 35 1 0.00% 36 3 0.00% 37 1 0.00% 38 5 0.00% 39 6 0.00% 40 5 0.00% 41 5 0.00% 42 4 0.00% 43 6 0.00% 44 3 0.00% 45 2 0.00% 46 10 0.00% 47 7 0.00% 48 12 0.00% 49 11 0.00% 50 12 0.00% 51 21 0.00% 52 19 0.00% 53 17 0.00% 54 16 0.00% 55 16 0.00% 56 18 0.00% 57 30 0.00% 58 31 0.00% 59 37 0.00% 60 36 0.00% 61 43 0.00% 62 61 0.00% 63 68 0.00% 64 72 0.00% 65 79 0.00% 66 80 0.00% 67 104 0.00% 68 106 0.00% 69 136 0.00% 70 154 0.00% 71 188 0.00% 72 190 0.00% 73 226 0.00% 74 240 0.00% 75 289 0.00% 76 291 0.00% 77 343 0.00% 78 402 0.00% 79 455 0.00% 80 532 0.01% 81 550 0.01% 82 674 0.01% 83 798 0.01% 84 947 0.01% 85 983 0.01% 86 1176 0.01% 87 1285 0.01% 88 1358 0.01% 89 1570 0.02% 90 1786 0.02% 91 2095 0.02% 92 2286 0.02% 93 2498 0.03% 94 2933 0.03% 95 3241 0.03% 96 3577 0.04% 97 3998 0.04% 98 4360 0.05% 99 4657 0.05% 100 5166 0.05% 101 5685 0.06% 102 6299 0.07% 103 6950 0.07% 104 7639 0.08% 105 8273 0.09% 106 9293 0.10% 107 10053 0.11% 108 11015 0.12% 109 11884 0.12% 110 12724 0.13% 111 13951 0.15% 112 15572 0.16% 113 17001 0.18% 114 18560 0.20% 115 20615 0.22% 116 22201 0.23% 117 23613 0.25% 118 25587 0.27% 119 27233 0.29% 120 29096 0.31% 121 30987 0.33% 122 32699 0.34% 123 34848 0.37% 124 37595 0.40% 125 40518 0.43% 126 8978473 94.42% 9508705 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=4.10 fanout-score-rank=25 prefix-density=0.33 prefix-fanout=3.2 sequence=GCAGGTGCAGCTGGTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=43.86 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=5.4 sequence=AATATCTTTATATATGAACAAAAACTTTTCATGCCCAGCAATTGCTTGGATGCAATGCGGTACTTAGGTACAAAGAGTGAAACATCAGAATAATTAAAGTGGCATGCTTAAAAGGTGTAAAGGCAGCTGCCGTCGTCACTCCTTGCTGTTGGGTCGTAGTTCTCGGCATTCCGGTCAGTGCAACCTTCTGGGACGGGCAAATTACCTTGTTGTGCTCCTTTACCTCCTCCTATGCAGCTAGAGATGGTGTGTGTATGAAGAGTGTTCTAACCGTAGAAGGAACCAGTCTTCATGGCATCTGAGTTAGCATCTCCCAGAGCAGCCTCGCTCATGTACTTGTCAGCAAGCTGCACACGCTTGACATTGTCCTGCTCTTGGACGAGCATGTGGCCGTACTCCAGGAGCTTCTCGATTGTCATCTTTGGCTGCTCAAAGGACACCGGTCCATCCTTCGAGTTCACCAGCTTCTTGCCGATGTTCTCTATTCCGGTTGAGCTGACCCACTTGCGCA criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=3.90 fanout-score-rank=22 prefix-density=0.34 prefix-fanout=2.8 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.08 sequence-density-rank=23 fanout-score=153.37 fanout-score-rank=1 prefix-density=0.56 prefix-fanout=21.0 sequence=GAAGAAGAAGAAA SRR7692619 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 10 11:05:19 Started mapping on | Apr 10 11:05:19 Finished on | Apr 10 11:05:56 Mapping speed, Million of reads per hour | 925.17 Number of input reads | 9508705 Average input read length | 250 UNIQUE READS: Uniquely mapped reads number | 9254937 Uniquely mapped reads % | 97.33% Average mapped length | 250.21 Number of splices: Total | 8736236 Number of splices: Annotated (sjdb) | 8284834 Number of splices: GT/AG | 8616099 Number of splices: GC/AG | 107443 Number of splices: AT/AC | 3976 Number of splices: Non-canonical | 8718 Mismatch rate per base, % | 0.19% Deletion rate per base | 0.01% Deletion average length | 2.19 Insertion rate per base | 0.01% Insertion average length | 2.07 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 102760 % of reads mapped to multiple loci | 1.08% Number of reads mapped to too many loci | 9360 % of reads mapped to too many loci | 0.10% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.99% % of reads unmapped: other | 0.50% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 151008 151008 151008 N_multimapping 102760 102760 102760 N_noFeature 303849 9031512 359243 N_ambiguous 194295 992 26261 UnstrandedReadsAssigned:8756793 PositiveStrandReadsAssigned:222433 NegativeStrandReadsAssigned:8869433 Dataset is classified negative stranded MeadianReadLen=126 20thPercentileLength=126 echo kmer=121 SRR7692619 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7692619-trimmed-pair1.fastq SRR7692619-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 9,508,705 reads, 8,933,332 reads pseudoaligned [quant] estimated average fragment length: 171.818 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,110 rounds 52973 SRR7692619.ke.tsv 35125 SRR7692619.se.tsv 88098 total ==> SRR7692619.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 765.387 0 0 PNS24247 1044 873.182 29.2283 5.87126 PNS24249 1928 1757.18 43.2521 4.31741 PNS24246 1044 873.182 29.2283 5.87126 PNS24248 1044 873.182 29.2283 5.87126 PNS24244 1471 1300.18 17.063 2.30189 PNS24243 293 124.24 0 0 KQK14069 1603 1432.18 524.581 64.2461 KQK14071 474 304.529 30.8333 17.7593 ==> SRR7692619.se.tsv <== BRADI_1g14170v3 610 BRADI_1g53295v3 86 BRADI_1g59795v3 198 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 70 BRADI_1g74790v3 92 BRADI_1g09890v3 0 BRADI_1g77505v3 162 BRADI_1g48960v3 0 SRR7692619 completed mapping pipeline successfully