Starting /dee2/code/volunteer_pipeline.sh SRR7804114 current disk space = 2825397784576 free memory = 1575303444 SRR7804114_1.fastq is conventional basespace SRR7804114_1.fastq read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7804114_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25056662 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.5056662E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.47741170556439 12.735629892081763 9.930608197565173 34.85635020478867 2 26.85813256902478 13.532054834412852 29.90927188721723 29.700540709345134 3 23.112286927557914 17.42200598863933 24.779706361353846 34.68600072244891 4 26.38595808424099 22.859559211415352 21.98838886525798 28.766093839085677 5 26.60621003479357 26.166705758102673 22.903323147210607 24.323761059893148 6 25.1498463761853 29.211927749993194 22.95255449428978 22.68567137953172 7 19.28772153640104 23.411191938143713 36.64400855325456 20.657077972200685 8 21.89597321462851 23.64225929215951 27.465980903601604 26.995786589610383 9 20.83063578061595 21.838196165155598 31.432470933279145 25.89869712094931 10-14 23.82276058957893 25.205914498906516 24.866792711654888 26.104532199859666 15-19 23.99017155597182 24.490722666889948 25.162603861599763 26.356501915538473 20-24 24.19717890909859 24.451402864055947 24.93821260381387 26.413205623031594 25-29 24.071278927735865 24.340168694457386 24.845379643944593 26.74317273386216 30-34 24.035083556102432 24.254352988074412 24.97211819333412 26.738445262489037 35-39 24.162018069286322 24.166803223829255 24.87177980849963 26.79939889838479 40-44 24.47981379163753 24.16582943091143 24.553541090189906 26.800815687261135 45-49 24.191897548045308 24.14980894103133 24.78348153477107 26.87481197615229 50-54 24.19595274415966 24.051075503210694 24.568873931713235 27.184097820916413 55-59 24.35453112188992 24.144431385996235 24.378073843768597 27.122963648345248 60-64 24.463303212534854 23.992278779990727 24.39841108923447 27.146006918239944 65-69 24.468599209264184 23.806261185149083 24.380565136728908 27.344574468857825 70-74 24.800351069960318 23.8360844144811 24.03975163775032 27.323812877808262 75-79 24.708524224016752 23.89383150876202 24.13571288945032 27.26193137777091 80-84 24.719176081794135 23.90783497019675 24.182731123563066 27.19025782444605 85-89 24.99834155698374 23.663103961504934 24.011015813129443 27.327538668381884 90-94 25.080157328225017 23.73909117419915 24.08464639402672 27.09610510354911 95-99 24.896363489249794 23.55258751985869 24.175856498170877 27.37519249272064 100-104 25.13678960848357 23.49745984448012 24.040655259376443 27.325095287659867 105-109 25.261514678869673 23.41325992142475 24.05021675050232 27.275008649203258 110-114 25.258958894442067 23.376056857549717 24.149528455546204 27.215455792462013 115-119 25.33131882623864 23.223420956537748 24.016064812876984 27.429195404346622 120-124 25.31195975536268 23.38923970471845 23.863827451053638 27.434973088865235 125-129 25.239778567673753 23.334946908389288 23.779607815854682 27.645666708082274 130-134 25.612658096197105 23.001981229819986 23.856952357455157 27.528408316527752 135-139 25.479519240610003 23.269701066448654 23.92604555879573 27.32473413414561 140-144 25.72975057502882 23.14701260310174 23.71959337018028 27.40364345168916 145-149 25.60131767944322 23.241729035857443 23.708104923627555 27.448848361071775 150-151 25.561376807877988 23.197599599786965 23.66688410723618 27.57413948509887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4354.0 1 2709.0 2 846.0 3 567.5 4 478.0 5 468.0 6 469.0 7 460.0 8 452.0 9 445.5 10 438.5 11 428.5 12 464.0 13 503.5 14 548.5 15 642.5 16 770.5 17 898.5 18 1057.5 19 1294.5 20 1646.5 21 2229.5 22 2805.0 23 3342.5 24 4486.0 25 6361.5 26 9522.5 27 14327.5 28 21951.5 29 33792.0 30 50335.0 31 73909.0 32 101265.0 33 137617.0 34 184047.0 35 237645.5 36 303612.0 37 383716.0 38 460598.0 39 531609.5 40 614896.0 41 702337.5 42 774331.0 43 821517.0 44 856958.0 45 887442.5 46 914738.5 47 916464.5 48 892287.0 49 886068.0 50 893820.5 51 875487.5 52 839823.0 53 830421.5 54 843652.0 55 906298.5 56 925829.0 57 817603.5 58 731878.5 59 685789.5 60 626842.5 61 568550.5 62 494892.5 63 447768.0 64 430551.0 65 404878.0 66 374525.0 67 353521.5 68 333128.0 69 300782.0 70 275783.0 71 248070.5 72 217512.0 73 184366.5 74 145438.0 75 118430.0 76 97824.5 77 76886.5 78 57369.0 79 40221.5 80 24970.0 81 15296.0 82 10002.0 83 5966.0 84 2708.0 85 837.0 86 378.5 87 210.0 88 111.5 89 51.5 90 27.5 91 24.0 92 17.0 93 13.0 94 14.0 95 11.0 96 11.0 97 16.5 98 31.5 99 30.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.990954581260664E-4 2 0.01159771401314349 3 8.740190532960854E-4 4 9.578290995025595E-5 5 9.179195536899529E-5 6 0.0 7 3.990954581260664E-6 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 5.82679368864057E-5 25-29 0.0 30-34 7.981909162521329E-7 35-39 0.0 40-44 0.0 45-49 0.0 50-54 1.5963818325042659E-6 55-59 3.432220939884171E-4 60-64 0.0 65-69 0.0 70-74 1.5165627408790524E-5 75-79 0.0 80-84 0.0 85-89 1.1094853735904647E-4 90-94 3.990954581260664E-6 95-99 7.981909162521329E-7 100-104 2.873487298507678E-5 105-109 7.502994612770048E-5 110-114 8.460823712272609E-5 115-119 4.62950731426237E-5 120-124 0.0012172411472845027 125-129 3.416257121559129E-4 130-134 1.1972863743781993E-5 135-139 1.2771054660034127E-5 140-144 1.915658199005119E-5 145-149 2.5542109320068254E-5 150-151 2.793668206882465E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.5056662E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.87264108673192 #Duplication Level Percentage of deduplicated Percentage of total 1 60.246915805050726 16.792406608167358 2 18.55902496286039 10.34578083419012 3 7.61367071336677 6.366393334387026 4 3.850650689455274 4.293112184702547 5 2.225955459841471 3.102162880360632 6 1.48812929635983 2.4886856260853074 7 0.9894304643076633 1.9304628148348217 8 0.7444579480771424 1.6600007352735278 9 0.543162662999118 1.3625440161739146 >10 3.1722925122616816 16.34784357094284 >50 0.28417631615186756 5.5565216484423345 >100 0.23499055469492516 13.20901168804301 >500 0.028142282976086085 5.444068868130255 >1k 0.01818756828286534 9.309465266555554 >5k 6.594117455222586E-4 1.2758825007042198 >10k+ 1.5335156872609826E-4 0.5156574230065375 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.97738645315166E-5 3.990954581260664E-6 1.1972863743781993E-5 0.0 0.0 2 9.97738645315166E-5 3.990954581260664E-6 1.5963818325042656E-5 0.0 7.981909162521328E-6 3 9.97738645315166E-5 3.990954581260664E-6 1.5963818325042656E-5 3.990954581260664E-6 1.9954772906303323E-5 4 1.1972863743781992E-4 3.990954581260664E-6 1.9954772906303323E-5 7.981909162521328E-6 2.793668206882465E-5 5 1.3968341034412325E-4 3.990954581260664E-6 1.9954772906303323E-5 7.981909162521328E-6 5.1882409556388636E-5 6 1.556472286691659E-4 3.990954581260664E-6 1.9954772906303323E-5 3.192763665008531E-5 5.1882409556388636E-5 7 1.795929561567299E-4 3.990954581260664E-6 1.9954772906303323E-5 3.192763665008531E-5 9.179195536899528E-5 8 2.0752963822555454E-4 3.990954581260664E-6 2.793668206882465E-5 3.5918591231345976E-5 1.3170150118160193E-4 9 2.234934565505972E-4 3.990954581260664E-6 4.390050039386731E-5 3.5918591231345976E-5 1.356924557628626E-4 10-11 2.733803888163555E-4 5.9864318718909966E-6 6.585075059080097E-5 4.1905023103236974E-5 1.8757486531925123E-4 12-13 3.073035027570712E-4 7.981909162521328E-6 9.578290995025595E-5 4.390050039386731E-5 2.6539847965383414E-4 14-15 3.551949577321991E-4 1.995477290630332E-5 9.578290995025595E-5 4.5895977684497644E-5 3.2127184379148344E-4 16-17 3.9510450354480575E-4 3.9909545812606646E-5 9.97738645315166E-5 6.18597960095403E-5 4.3700952664804275E-4 18-19 4.489823903918248E-4 3.9909545812606646E-5 1.0376481911277727E-4 6.385527330017063E-5 4.8091002704191E-4 20-21 5.28801482017038E-4 3.9909545812606646E-5 1.0576029640340761E-4 6.78462278814313E-5 5.128376636919954E-4 22-23 6.106160509328816E-4 4.1905023103236974E-5 1.0975125098466827E-4 7.582813704395262E-5 5.746974597015356E-4 24-25 6.764668015236826E-4 4.390050039386731E-5 1.1374220556592893E-4 7.981909162521329E-5 6.90435142558095E-4 26-27 7.423175521144836E-4 4.390050039386731E-5 1.1972863743781994E-4 7.981909162521329E-5 7.483039839863747E-4 28-29 8.580552349710428E-4 4.390050039386731E-5 1.476653195066446E-4 8.780100078773462E-5 7.702542341833082E-4 30-31 9.538381449212987E-4 4.390050039386731E-5 1.536517513785356E-4 9.179195536899528E-5 7.922044843802419E-4 32-33 0.001033657236546512 4.390050039386731E-5 1.6362913783168724E-4 9.179195536899528E-5 8.201411664490665E-4 34-35 0.0011254491919155073 6.385527330017063E-5 1.6362913783168724E-4 9.179195536899528E-5 8.400959393553699E-4 36-37 0.0012212321018657633 6.585075059080097E-5 1.756020015754692E-4 9.578290995025595E-5 8.760145305867159E-4 38-39 0.0012910738070378248 7.582813704395262E-5 1.9555677448177255E-4 1.0376481911277727E-4 8.899828716211281E-4 40-41 0.0013569245576286258 7.582813704395262E-5 2.035386836442939E-4 1.0775577369403794E-4 8.939738262023888E-4 42-43 0.0014706667631945547 7.582813704395262E-5 2.035386836442939E-4 1.1972863743781994E-4 9.039512126555405E-4 44-45 0.0016243185145730903 7.582813704395262E-5 2.0752963822555454E-4 1.237195920190806E-4 9.119331218180619E-4 46-47 0.001718105947232716 7.582813704395262E-5 2.115205928068152E-4 1.237195920190806E-4 9.239059855618438E-4 48-49 0.0018597848348674696 7.582813704395262E-5 2.115205928068152E-4 1.237195920190806E-4 9.55833622211929E-4 50-51 0.0020513506547679815 7.582813704395262E-5 2.115205928068152E-4 1.237195920190806E-4 0.0010396436684184031 52-53 0.002254889338412275 7.782361433458296E-5 2.3347084300374886E-4 1.237195920190806E-4 0.001117467282752986 54-55 0.0024723963630909817 7.981909162521329E-5 2.5143013861942185E-4 1.297060238909716E-4 0.0011394175329499196 56-57 0.0026958898196415785 8.381004620647395E-5 2.5941204778194316E-4 1.3968341034412325E-4 0.0011813225560531565 58-59 0.002921378753482806 8.381004620647395E-5 2.5941204778194316E-4 1.3968341034412325E-4 0.0012172411472845027 60-61 0.0032087274833335743 8.381004620647395E-5 2.5941204778194316E-4 1.3968341034412325E-4 0.0012771054660034127 62-63 0.0035339902817063183 8.381004620647395E-5 2.713849115257252E-4 1.3968341034412325E-4 0.001317015011816019 64-65 0.003929094785251124 9.378743265962562E-5 2.973261163039195E-4 1.4566984221601423E-4 0.00132300144368791 66-67 0.004435946017071229 1.0376481911277727E-4 3.0131707088518016E-4 1.476653195066446E-4 0.001332978830141062 68-69 0.005058534931747892 1.0775577369403794E-4 3.092989800477015E-4 1.476653195066446E-4 0.0013609155122098864 70-71 0.005806838915734266 1.0775577369403794E-4 3.3124923024463514E-4 1.536517513785356E-4 0.0013848612396974505 72-73 0.006870428311640234 1.0975125098466827E-4 3.3124923024463514E-4 1.6362913783168724E-4 0.0014108024444756447 74-75 0.00824132121030327 1.117467282752986E-4 3.3324470753526547E-4 1.7759747886609957E-4 0.0014267662628006873 76-77 0.009863644247585733 1.117467282752986E-4 3.5519495773219913E-4 1.8757486531925123E-4 0.0014347481719632089 78-79 0.012056673789988467 1.117467282752986E-4 3.591859123134598E-4 1.915658199005119E-4 0.001448716512997621 80-81 0.014888256065392909 1.117467282752986E-4 3.990954581260664E-4 1.9555677448177255E-4 0.0014706667631945547 82-83 0.018725558895275038 1.117467282752986E-4 4.4499143581056406E-4 2.0353868364429386E-4 0.0014966079679727493 84-85 0.02366636066687574 1.117467282752986E-4 4.5297334497308543E-4 2.234934565505972E-4 0.0015185582181696828 86-87 0.0299002317228049 1.117467282752986E-4 4.569642995543461E-4 2.2748441113185788E-4 0.001550485854819768 88-89 0.037520959495722137 1.117467282752986E-4 4.589597768449764E-4 2.294798884224882E-4 0.0016083546962480477 90-91 0.046323009824692535 1.117467282752986E-4 4.6694168600749773E-4 2.3147536571311854E-4 0.0016382868556075027 92-93 0.057647343449019665 1.117467282752986E-4 4.7492359517001904E-4 2.3945727487563985E-4 0.001706133083488934 94-95 0.07241587087697476 1.117467282752986E-4 4.829055043325404E-4 2.3945727487563985E-4 0.0017640019249172136 96-97 0.09033326146954451 1.117467282752986E-4 4.8689645891380107E-4 2.3945727487563985E-4 0.0019435948810739433 98-99 0.11191634384500218 1.117467282752986E-4 4.908874134950617E-4 2.3945727487563985E-4 0.0020333913591523084 100-101 0.137260900913298 1.117467282752986E-4 4.92882890785692E-4 2.3945727487563985E-4 0.002105228541615 102-103 0.1668358698377302 1.117467282752986E-4 5.068512318201043E-4 2.3945727487563985E-4 0.002240920997377863 104-105 0.20197223397114905 1.117467282752986E-4 5.088467091107346E-4 2.3945727487563985E-4 0.0023227355662937066 106-107 0.2421192415813407 1.117467282752986E-4 5.327924365982987E-4 2.3945727487563985E-4 0.002372622498559465 108-109 0.287833630832391 1.117467282752986E-4 5.76692936992166E-4 2.3945727487563985E-4 0.0024504461128940476 110-111 0.3389058766087837 1.117467282752986E-4 5.986431871890997E-4 2.3945727487563985E-4 0.002608088818853844 112-113 0.3960723100307615 1.117467282752986E-4 6.026341417703603E-4 2.3945727487563985E-4 0.0026639621829914932 114-115 0.462605513854958 1.1374220556592893E-4 6.205934373860332E-4 2.3945727487563985E-4 0.0027357993654541853 116-117 0.5385753297865454 1.1972863743781994E-4 6.305708238391849E-4 2.3945727487563985E-4 0.002783690820429313 118-119 0.6219603393301151 1.237195920190806E-4 6.345617784204457E-4 2.3945727487563985E-4 0.0028734872985076783 120-121 0.7126348274163574 1.237195920190806E-4 6.345617784204457E-4 2.3945727487563985E-4 0.002903419457867133 122-123 0.8117262387144784 1.237195920190806E-4 6.345617784204457E-4 2.3945727487563985E-4 0.003013170708851802 124-125 0.9245305699538111 1.2571506930971092E-4 6.345617784204457E-4 2.3945727487563985E-4 0.0031169355279645787 126-127 1.050930087974208 1.2771054660034125E-4 6.385527330017063E-4 2.3945727487563985E-4 0.003178795323974119 128-129 1.1887177948922325 1.2771054660034125E-4 6.465346421642277E-4 2.414527521662702E-4 0.0032646008474712233 130-131 1.3352796154571587 1.2771054660034125E-4 6.465346421642277E-4 2.474391840381612E-4 0.0033304515980620243 132-133 1.4957199805784187 1.297060238909716E-4 6.505255967454883E-4 2.474391840381612E-4 0.0033743520984558916 134-135 1.6725851192788568 1.3170150118160193E-4 7.024080063018768E-4 2.673939569444645E-4 0.00342224355343102 136-137 1.8689221253812658 1.3170150118160193E-4 7.303446883707015E-4 2.673939569444645E-4 0.003450180235499844 138-139 2.077802701732577 1.3170150118160193E-4 7.423175521144836E-4 2.713849115257252E-4 0.003478116917568669 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGGG 7450 0.0 19.657612 8 AGTTACG 9400 0.0 17.122248 6 GTTACGA 6575 0.0 16.983099 1 CCACATG 22315 0.0 16.149088 2 TTACGAC 7160 0.0 14.582701 2 TACGACT 7455 0.0 14.10131 3 GTTACGG 11530 0.0 14.084919 7 TTTTTTT 182390 0.0 13.119124 1 CGAATTA 5125 0.0 13.0146675 3 GAGTTTA 11515 0.0 12.844098 4 GTGCGAC 11255 0.0 12.755917 1 ACATGCT 27200 0.0 12.500901 4 GTCCGTT 4360 0.0 12.14027 1 ACCACAT 23190 0.0 11.912865 1 TAAGCCG 5865 0.0 11.743328 8 CCTAGAT 19280 0.0 11.583389 1 TGCGACG 12935 0.0 11.491479 2 GTCCGTA 10600 0.0 11.353711 6 CGGCTAG 27710 0.0 11.198061 145 CCTATAG 14150 0.0 11.118313 8 >>END_MODULE SRR7804114 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7804114_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25056662 Sequences flagged as poor quality 0 Sequence length 151 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0 30.0 30.0 30.0 30.0 30.0 2 30.0 30.0 30.0 30.0 30.0 30.0 3 30.0 30.0 30.0 30.0 30.0 30.0 4 30.0 30.0 30.0 30.0 30.0 30.0 5 30.0 30.0 30.0 30.0 30.0 30.0 6 30.0 30.0 30.0 30.0 30.0 30.0 7 30.0 30.0 30.0 30.0 30.0 30.0 8 30.0 30.0 30.0 30.0 30.0 30.0 9 30.0 30.0 30.0 30.0 30.0 30.0 10-14 30.0 30.0 30.0 30.0 30.0 30.0 15-19 30.0 30.0 30.0 30.0 30.0 30.0 20-24 30.0 30.0 30.0 30.0 30.0 30.0 25-29 30.0 30.0 30.0 30.0 30.0 30.0 30-34 30.0 30.0 30.0 30.0 30.0 30.0 35-39 30.0 30.0 30.0 30.0 30.0 30.0 40-44 30.0 30.0 30.0 30.0 30.0 30.0 45-49 30.0 30.0 30.0 30.0 30.0 30.0 50-54 30.0 30.0 30.0 30.0 30.0 30.0 55-59 30.0 30.0 30.0 30.0 30.0 30.0 60-64 30.0 30.0 30.0 30.0 30.0 30.0 65-69 30.0 30.0 30.0 30.0 30.0 30.0 70-74 30.0 30.0 30.0 30.0 30.0 30.0 75-79 30.0 30.0 30.0 30.0 30.0 30.0 80-84 30.0 30.0 30.0 30.0 30.0 30.0 85-89 30.0 30.0 30.0 30.0 30.0 30.0 90-94 30.0 30.0 30.0 30.0 30.0 30.0 95-99 30.0 30.0 30.0 30.0 30.0 30.0 100-104 30.0 30.0 30.0 30.0 30.0 30.0 105-109 30.0 30.0 30.0 30.0 30.0 30.0 110-114 30.0 30.0 30.0 30.0 30.0 30.0 115-119 30.0 30.0 30.0 30.0 30.0 30.0 120-124 30.0 30.0 30.0 30.0 30.0 30.0 125-129 30.0 30.0 30.0 30.0 30.0 30.0 130-134 30.0 30.0 30.0 30.0 30.0 30.0 135-139 30.0 30.0 30.0 30.0 30.0 30.0 140-144 30.0 30.0 30.0 30.0 30.0 30.0 145-149 30.0 30.0 30.0 30.0 30.0 30.0 150-151 30.0 30.0 30.0 30.0 30.0 30.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.5056662E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.21713393761289 18.389217231659703 11.127395464217281 30.266253366510128 2 32.75380585699821 22.493316053641127 22.996954500150018 21.755923589210642 3 26.58915221828031 24.968441526648682 24.789854291046428 23.652551964024575 4 28.522981233493912 29.95731434618067 18.701329810012204 22.818374610313217 5 29.955921503031806 30.416761019484557 17.89873287990236 21.728584597581275 6 27.081907398519405 33.07393458873333 17.911483979789487 21.932674032957785 7 25.144770885474326 19.708847080622593 30.704234694319403 24.442147339583677 8 27.05209895875197 22.410379323470938 20.847234160719413 29.690287557057683 9 26.228441302870042 22.65799607457517 24.324272226164624 26.789290396390165 10-14 28.22229074247799 24.732248054429597 20.963552128372086 26.08190907472033 15-19 27.95008688707219 24.511554651613213 21.978006487855406 25.560351973459195 20-24 27.866365440057418 24.70761268999039 21.927235958245355 25.498785911706833 25-29 27.888451382710116 24.57062317398862 21.8218859319729 25.71903951132836 30-34 27.67272432377465 24.737055558318183 22.053957546300463 25.536262571606706 35-39 27.788009875984436 24.776061947534146 21.82047428058014 25.61545389590128 40-44 27.903128090684685 24.636773322851866 21.889896433200757 25.570202153262688 45-49 27.789738313906298 24.616671606138123 21.965495643434068 25.628094436521515 50-54 27.875023639421915 24.468082975043387 22.06681737628753 25.59007600924717 55-59 27.851790696237117 24.341323072812514 22.120312131921736 25.686574099028626 60-64 27.767963665710937 24.212644126340532 22.345103270339838 25.67428893760869 65-69 27.833407338934453 24.41360944247083 22.153156713372276 25.599826505222445 70-74 27.96551878546612 24.208344943687223 22.202795759913393 25.62334051093326 75-79 27.674449793536482 24.23032029079217 22.332587864647593 25.76264205102375 80-84 27.75446097945772 24.397371221967685 22.23414524385352 25.61402255472108 85-89 27.68582387687273 24.28322454576311 22.2462237347692 25.78472784259496 90-94 27.708798562234666 24.441162194708937 22.341319845396804 25.50871939765959 95-99 27.864266536659414 24.435039286850028 22.284743384210383 25.415950792280174 100-104 27.804537972702363 24.52766390630145 22.29224866927796 25.375549451718232 105-109 27.61177080190703 24.487110557187368 22.591132318638536 25.30998632226707 110-114 27.63930430186682 24.616556387501642 22.321338389855068 25.422800920776474 115-119 27.699459728514515 24.570497858014768 22.337047927613025 25.392994485857695 120-124 27.61034077748283 24.686269656020492 22.318204770887792 25.385184795608883 125-129 27.621334702177595 24.773979262440434 22.27939389051079 25.325292144871174 130-134 27.75055995292717 24.68555206229044 22.370969014792628 25.19291896998977 135-139 27.580030357277536 24.79702023820365 22.615150879283572 25.00779852523524 140-144 27.939739272631574 24.90936529967971 22.486500318998832 24.66439510868988 145-149 27.81159036845583 24.89048313280407 22.45725131586464 24.840675182875458 150-151 27.939044948528657 25.13764005258482 22.39548287760619 24.52783212128033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3190.0 1 2262.5 2 1375.5 3 1607.0 4 2017.0 5 2565.0 6 3210.5 7 3771.0 8 4221.0 9 4591.5 10 4923.0 11 5224.0 12 5538.5 13 5868.5 14 6039.5 15 6332.0 16 6625.5 17 6564.0 18 6683.5 19 6883.5 20 7189.5 21 7849.0 22 8386.0 23 8796.5 24 9194.5 25 10454.0 26 12939.0 27 16170.5 28 21057.5 29 27131.0 30 36576.5 31 49094.5 32 60633.0 33 73708.0 34 98770.0 35 137551.5 36 181220.0 37 238129.5 38 303156.5 39 383754.5 40 485797.5 41 559654.5 42 630298.0 43 706544.5 44 763639.5 45 813788.5 46 849035.0 47 870879.0 48 861192.0 49 851732.5 50 838956.5 51 833223.5 52 811730.5 53 822749.0 54 910427.0 55 946160.5 56 873094.0 57 778522.5 58 759032.0 59 755632.0 60 710426.0 61 647141.5 62 603909.0 63 561774.5 64 518575.0 65 486311.5 66 461294.0 67 464673.0 68 469650.0 69 435986.5 70 383726.5 71 338781.5 72 294628.5 73 251249.5 74 208709.0 75 164297.5 76 129521.0 77 101719.5 78 78042.0 79 54667.0 80 34883.0 81 24281.0 82 16990.0 83 10442.0 84 6006.0 85 4415.0 86 3804.0 87 3484.5 88 3253.0 89 3120.5 90 3137.0 91 3039.5 92 2996.5 93 3070.0 94 3140.5 95 3241.0 96 3357.5 97 3584.5 98 4110.0 99 5451.0 100 23619.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06491287626420471 2 0.011873089879250476 3 0.0 4 0.0 5 0.0 6 0.0 7 3.990954581260664E-6 8 0.0 9 4.390050039386731E-5 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 1.5963818325042659E-6 40-44 4.733272133375148E-4 45-49 0.0 50-54 7.981909162521329E-7 55-59 7.981909162521329E-7 60-64 0.0 65-69 0.0 70-74 1.5963818325042656E-5 75-79 1.26114164767837E-4 80-84 3.990954581260664E-6 85-89 1.5963818325042659E-6 90-94 0.0 95-99 3.272582756633745E-5 100-104 7.183718246269195E-6 105-109 9.658110086650807E-5 110-114 2.809632025207508E-4 115-119 0.0 120-124 2.394572748756399E-6 125-129 3.990954581260664E-6 130-134 8.780100078773462E-6 135-139 5.587336413764931E-6 140-144 5.746974597015357E-4 145-149 7.742451887645688E-5 150-151 7.981909162521328E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 2.5056662E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.6602796630621 #Duplication Level Percentage of deduplicated Percentage of total 1 60.87543870230061 16.229562204149282 2 17.335186693098574 9.243218504988011 3 7.8857645641672445 6.307100659132912 4 3.955900085024607 4.218616103435487 5 2.376213918932274 3.1675263808997594 6 1.4873969686671704 2.3792651492794548 7 1.0344823022046041 1.930571123828415 8 0.7585944929606742 1.6179473066552292 9 0.5696669330553537 1.3668731775049143 >10 3.115361360512797 15.2938133922233 >50 0.29586280971941853 5.524359146643491 >100 0.25161448606962267 13.709906425390653 >500 0.035912866616113405 6.673574861719768 >1k 0.021790299837860575 10.38416609275771 >5k 6.699550389305724E-4 1.1591486199621075 >10k+ 1.4356179405654424E-4 0.7943508514293344 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 61456 0.2452681047459554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.981909162521329E-5 0.0 3.990954581260664E-6 0.0 0.0 2 7.981909162521329E-5 0.0 1.5963818325042656E-5 0.0 0.0 3 7.981909162521329E-5 0.0 1.5963818325042656E-5 0.0 3.990954581260664E-6 4 8.780100078773462E-5 0.0 1.5963818325042656E-5 0.0 3.990954581260664E-6 5 9.97738645315166E-5 0.0 2.793668206882465E-5 0.0 3.990954581260664E-6 6 1.1573768285655927E-4 0.0 3.192763665008531E-5 0.0 3.990954581260664E-6 7 1.3170150118160193E-4 0.0 3.192763665008531E-5 0.0 3.990954581260664E-6 8 1.5963818325042658E-4 0.0 3.192763665008531E-5 3.990954581260664E-6 3.990954581260664E-6 9 1.6362913783168724E-4 0.0 3.5918591231345976E-5 3.990954581260664E-6 3.990954581260664E-6 10-11 2.0154320635366353E-4 1.995477290630332E-6 3.9909545812606646E-5 3.990954581260664E-6 3.990954581260664E-6 12-13 2.2748441113185785E-4 3.990954581260664E-6 5.387788684701897E-5 3.990954581260664E-6 3.990954581260664E-6 14-15 2.5741657049131283E-4 3.990954581260664E-6 6.385527330017063E-5 3.990954581260664E-6 7.981909162521328E-6 16-17 2.9133968443202846E-4 3.990954581260664E-6 7.183718246269195E-5 3.990954581260664E-6 1.596381832504266E-5 18-19 3.3324470753526547E-4 3.990954581260664E-6 7.183718246269195E-5 3.990954581260664E-6 1.9954772906303323E-5 20-21 4.0308641270732706E-4 3.990954581260664E-6 7.582813704395262E-5 3.990954581260664E-6 2.1950250196933655E-5 22-23 4.8091002704191E-4 3.990954581260664E-6 7.582813704395262E-5 3.990954581260664E-6 2.3945727487563986E-5 24-25 5.607291186671234E-4 7.981909162521328E-6 7.582813704395262E-5 3.990954581260664E-6 2.3945727487563986E-5 26-27 6.30570823839185E-4 7.981909162521328E-6 7.782361433458296E-5 3.990954581260664E-6 2.793668206882465E-5 28-29 7.443130294051139E-4 7.981909162521328E-6 8.979647807836495E-5 3.990954581260664E-6 3.392311394071565E-5 30-31 8.480778485178912E-4 7.981909162521328E-6 1.0775577369403794E-4 3.990954581260664E-6 3.5918591231345976E-5 32-33 9.438607584681472E-4 7.981909162521328E-6 1.117467282752986E-4 3.990954581260664E-6 3.5918591231345976E-5 34-35 0.001045630100290294 7.981909162521328E-6 1.1573768285655927E-4 3.990954581260664E-6 3.5918591231345976E-5 36-37 0.0011693496923093746 7.981909162521328E-6 1.1972863743781992E-4 3.990954581260664E-6 3.5918591231345976E-5 38-39 0.0012671280795502609 1.1972863743781993E-5 1.2172411472845027E-4 7.981909162521328E-6 3.5918591231345976E-5 40-41 0.0013649064667911473 1.1972863743781993E-5 1.2771054660034125E-4 1.1972863743781993E-5 4.98869322657583E-5 42-43 0.0015025943998446401 1.9954772906303323E-5 1.297060238909716E-4 1.1972863743781993E-5 5.387788684701897E-5 44-45 0.00168218735600137 1.9954772906303323E-5 1.3170150118160193E-4 1.1972863743781993E-5 5.58733641376493E-5 46-47 0.0018278571982173844 2.1950250196933655E-5 1.3369697847223226E-4 1.1972863743781993E-5 5.58733641376493E-5 48-49 0.002001463722502223 2.3945727487563986E-5 1.3968341034412325E-4 1.1972863743781993E-5 5.986431871890996E-5 50-51 0.002260875770284166 2.3945727487563986E-5 1.3968341034412325E-4 1.1972863743781993E-5 6.78462278814313E-5 52-53 0.0025143013861942186 2.3945727487563986E-5 1.4766531950664456E-4 1.1972863743781993E-5 7.582813704395262E-5 54-55 0.0027856862977199436 2.3945727487563986E-5 1.6562461512231757E-4 1.3968341034412325E-5 8.580552349710428E-5 56-57 0.003041107390920626 2.3945727487563986E-5 1.795929561567299E-4 1.5963818325042656E-5 8.979647807836495E-5 58-59 0.00330650587057446 2.3945727487563986E-5 1.795929561567299E-4 1.5963818325042656E-5 1.0376481911277727E-4 60-61 0.0036397505781097256 2.3945727487563986E-5 1.915658199005119E-4 1.5963818325042656E-5 1.0376481911277727E-4 62-63 0.003996941013132555 2.3945727487563986E-5 1.915658199005119E-4 1.5963818325042656E-5 1.0376481911277727E-4 64-65 0.004463882699140053 2.3945727487563986E-5 2.0353868364429386E-4 1.5963818325042656E-5 1.0775577369403794E-4 66-67 0.005022616340516546 2.3945727487563986E-5 2.234934565505972E-4 1.5963818325042656E-5 1.117467282752986E-4 68-69 0.0057190379149465315 2.3945727487563986E-5 2.2748441113185788E-4 1.5963818325042656E-5 1.3170150118160193E-4 70-71 0.00648929214912984 2.3945727487563986E-5 2.2748441113185788E-4 1.9954772906303323E-5 1.3768793305349292E-4 72-73 0.007604763954592196 2.3945727487563986E-5 2.2748441113185788E-4 1.9954772906303323E-5 1.3968341034412325E-4 74-75 0.009047494035717925 2.793668206882465E-5 2.3147536571311854E-4 1.9954772906303323E-5 1.5165627408790524E-4 76-77 0.010709726618812993 2.793668206882465E-5 2.4344822945690053E-4 1.9954772906303323E-5 1.5165627408790524E-4 78-79 0.012940670229737704 2.793668206882465E-5 2.4344822945690053E-4 1.9954772906303323E-5 1.5764270595979626E-4 80-81 0.0157822298915953 3.392311394071565E-5 2.5143013861942185E-4 1.9954772906303323E-5 1.6362913783168724E-4 82-83 0.019617537244186793 3.5918591231345976E-5 2.673939569444645E-4 1.9954772906303323E-5 1.676200924129479E-4 84-85 0.02463217167554082 3.791406852197631E-5 2.813622979788768E-4 1.9954772906303323E-5 1.915658199005119E-4 86-87 0.030909943231863846 3.9909545812606646E-5 2.893442071413982E-4 1.9954772906303323E-5 1.915658199005119E-4 88-89 0.03857656698246558 3.9909545812606646E-5 2.973261163039195E-4 2.3945727487563986E-5 1.915658199005119E-4 90-91 0.047482382130548756 3.9909545812606646E-5 2.9932159359454983E-4 2.3945727487563986E-5 1.915658199005119E-4 92-93 0.05883265695965408 3.9909545812606646E-5 3.112944573383318E-4 2.3945727487563986E-5 1.9555677448177255E-4 94-95 0.07370095825214068 3.9909545812606646E-5 3.112944573383318E-4 2.793668206882465E-5 1.995477290630332E-4 96-97 0.09166025386781368 4.1905023103236974E-5 3.112944573383318E-4 2.793668206882465E-5 2.0952511551618487E-4 98-99 0.11329122769824648 4.789145497512797E-5 3.212718437914835E-4 2.793668206882465E-5 2.1351607009744556E-4 100-101 0.13866771240319242 4.98869322657583E-5 3.3124923024463514E-4 2.793668206882465E-5 2.1950250196933654E-4 102-103 0.16832649137383104 5.1882409556388636E-5 3.531994804415688E-4 2.793668206882465E-5 2.234934565505972E-4 104-105 0.20345088264350614 5.1882409556388636E-5 3.5719043502282946E-4 2.793668206882465E-5 2.234934565505972E-4 106-107 0.24354600784414143 5.387788684701897E-5 3.591859123134598E-4 2.793668206882465E-5 2.2748441113185788E-4 108-109 0.28931028402745745 5.58733641376493E-5 3.591859123134598E-4 2.793668206882465E-5 2.294798884224882E-4 110-111 0.34041445744050025 6.585075059080097E-5 3.591859123134598E-4 2.793668206882465E-5 2.3147536571311854E-4 112-113 0.39762878231745313 7.183718246269195E-5 3.591859123134598E-4 2.793668206882465E-5 2.3347084300374886E-4 114-115 0.4643136424157376 7.582813704395262E-5 3.611813896040901E-4 2.793668206882465E-5 2.3746179758500952E-4 116-117 0.5404311236668315 7.582813704395262E-5 3.8911807167291477E-4 2.793668206882465E-5 2.4344822945690053E-4 118-119 0.6238939568247359 7.582813704395262E-5 3.911135489635451E-4 2.793668206882465E-5 2.494346613287915E-4 120-121 0.7146362911388595 7.782361433458296E-5 3.911135489635451E-4 2.793668206882465E-5 2.5143013861942185E-4 122-123 0.8139112863477186 7.981909162521329E-5 4.0508188999795745E-4 2.793668206882465E-5 2.653984796538342E-4 124-125 0.9271107220905961 7.981909162521329E-5 4.1106832186984843E-4 2.793668206882465E-5 2.673939569444645E-4 126-127 1.0537796295452284 7.981909162521329E-5 4.1106832186984843E-4 2.793668206882465E-5 2.673939569444645E-4 128-129 1.1919085630799504 7.981909162521329E-5 4.1306379916047876E-4 2.793668206882465E-5 2.673939569444645E-4 130-131 1.3385681620321175 8.381004620647395E-5 4.3700952664804275E-4 2.993215935945498E-5 2.9333516172265885E-4 132-133 1.499172156291209 8.780100078773462E-5 4.4299595851993374E-4 3.5918591231345976E-5 3.092989800477015E-4 134-135 1.6764084537677046 8.780100078773462E-5 4.5097786768245505E-4 3.5918591231345976E-5 3.232673210821138E-4 136-137 1.8730208357362206 8.780100078773462E-5 4.5496882226371576E-4 3.5918591231345976E-5 3.232673210821138E-4 138-139 2.0820430909751666 8.780100078773462E-5 4.6295073142623707E-4 3.5918591231345976E-5 3.292537529540048E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGTCA 8880 0.0 32.24915 8 TAGTCAT 9925 0.0 28.85365 9 TTCGATC 13610 0.0 27.223703 2 GCCAGTA 12625 0.0 22.453266 4 TTGCGGT 12190 0.0 21.291855 4 CCTTACC 12225 0.0 19.348219 1 GTTCGAT 21170 0.0 18.610209 1 ACATGCG 9285 0.0 16.709599 9 CGGGACT 12280 0.0 16.47176 3 TACCCGC 12510 0.0 16.458687 9 CTCGCTT 10880 0.0 16.392319 8 TTACCAG 19920 0.0 15.651773 2 GGCTTGC 18630 0.0 14.877254 1 TCGCTTG 11590 0.0 14.82515 9 TGCCAGT 18925 0.0 14.787188 3 TCGATCC 27865 0.0 14.622157 3 GTGGATA 18780 0.0 14.592523 9 AGTAGTC 19930 0.0 14.587176 7 CGATCCT 25025 0.0 14.572298 4 CCTGGCT 25780 0.0 14.229898 8 >>END_MODULE skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7804114 SRR7804114_1.fastq SRR7804114_2.fastq Input file: SRR7804114_1.fastq Paired file: SRR7804114_2.fastq trimmed: SRR7804114-trimmed-pair1.fastq, SRR7804114-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Apr 10 11:09:04 2025 >> started Thu Apr 10 11:09:32 2025 >> done (28.122s) 25056662 read pairs processed; of these: 76 ( 0.00%) short read pairs filtered out after trimming by size control 830 ( 0.00%) empty read pairs filtered out after trimming by size control 25055756 (100.00%) read pairs available; of these: 960891 ( 3.84%) trimmed read pairs available after processing 24094865 (96.16%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 10 0.00% 20 9 0.00% 21 12 0.00% 22 12 0.00% 23 10 0.00% 24 9 0.00% 25 8 0.00% 26 12 0.00% 27 18 0.00% 28 11 0.00% 29 12 0.00% 30 12 0.00% 31 12 0.00% 32 9 0.00% 33 16 0.00% 34 11 0.00% 35 17 0.00% 36 11 0.00% 37 12 0.00% 38 8 0.00% 39 10 0.00% 40 13 0.00% 41 15 0.00% 42 18 0.00% 43 21 0.00% 44 19 0.00% 45 12 0.00% 46 15 0.00% 47 22 0.00% 48 20 0.00% 49 27 0.00% 50 33 0.00% 51 27 0.00% 52 26 0.00% 53 36 0.00% 54 21 0.00% 55 29 0.00% 56 34 0.00% 57 31 0.00% 58 29 0.00% 59 40 0.00% 60 50 0.00% 61 32 0.00% 62 49 0.00% 63 48 0.00% 64 64 0.00% 65 58 0.00% 66 77 0.00% 67 93 0.00% 68 72 0.00% 69 94 0.00% 70 109 0.00% 71 130 0.00% 72 166 0.00% 73 177 0.00% 74 182 0.00% 75 208 0.00% 76 222 0.00% 77 285 0.00% 78 309 0.00% 79 338 0.00% 80 420 0.00% 81 469 0.00% 82 552 0.00% 83 620 0.00% 84 721 0.00% 85 752 0.00% 86 915 0.00% 87 946 0.00% 88 1030 0.00% 89 1088 0.00% 90 1253 0.01% 91 1394 0.01% 92 1659 0.01% 93 1881 0.01% 94 2040 0.01% 95 2212 0.01% 96 2557 0.01% 97 2674 0.01% 98 2967 0.01% 99 3196 0.01% 100 3414 0.01% 101 3685 0.01% 102 4149 0.02% 103 4431 0.02% 104 4713 0.02% 105 5068 0.02% 106 5399 0.02% 107 5846 0.02% 108 6027 0.02% 109 6462 0.03% 110 6862 0.03% 111 7102 0.03% 112 7858 0.03% 113 8474 0.03% 114 8877 0.04% 115 9684 0.04% 116 10255 0.04% 117 10470 0.04% 118 11017 0.04% 119 11325 0.05% 120 12243 0.05% 121 12382 0.05% 122 13207 0.05% 123 14440 0.06% 124 15126 0.06% 125 16069 0.06% 126 16820 0.07% 127 17585 0.07% 128 17992 0.07% 129 18495 0.07% 130 19393 0.08% 131 20296 0.08% 132 21325 0.09% 133 22358 0.09% 134 23816 0.10% 135 25152 0.10% 136 25619 0.10% 137 26578 0.11% 138 27112 0.11% 139 28125 0.11% 140 28708 0.11% 141 30389 0.12% 142 31526 0.13% 143 33029 0.13% 144 34985 0.14% 145 36391 0.15% 146 37246 0.15% 147 38760 0.15% 148 39424 0.16% 149 40298 0.16% 150 42035 0.17% 151 24094865 96.16% 25055756 reads passed initial QC criterion=sequence-density sequence-density=0.99 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=31 prefix-density=0.98 prefix-fanout=1.9 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=30 fanout-score=73.14 fanout-score-rank=1 prefix-density=0.69 prefix-fanout=3.4 sequence=GCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGA criterion=sequence-density sequence-density=0.83 sequence-density-rank=1 fanout-score=2.70 fanout-score-rank=19 prefix-density=0.92 prefix-fanout=2.4 sequence=GGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCAGGAATCGCTAGTAATCGCCGGTCAGCCATACGGCGGTGAATCCGTTCCCGGGCCTTGTACACACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=52.71 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=2.2 sequence=GCCTCGGCACCCCGGCCCCGTCCTCTTCCGGCAGCTTCCGGCCCAGGCTCATCAGGAACGCCCCCGTCCAGGCCGCGCCCGTCGCGCCCGCATTGATGGACGCCGCCGTGGAGCGCCTCAAGACCGGGTTCGAGAAGTTCAAGACCGAGGTCTACGACAAGAAGCCGGATGTCTTCGAGCCGCTCAAGGCCGGCCAGGCCCCCAAGTACATGGTGTTCGCCTGCGCCGACTCACGTGTGTGCCCGTCGGTGACCCTGGGCCTGGAGCCCGGTGAGGCCTTCACCGTCCGCAACATCGCCAACATGGTCCCGTCCTACTGCAAGAACAAGTACGCCGGTGTTGGGTCGGCCATCGAGTACGCCGTGTGTGCCCTCAAGGTTGAGGTCATCGTGGTGATTGGCCACAGCCGCTGCGGTGGAATCAAGGCACTCCTCTCGCTCAAGGATGGTGCAGATGACAGCTTCCACTTCGTCGAGGACTGGGTCAGGATCGGGTTCCCGGCCAAGAAGAA SRR7804114 testing PE reads STAR mapping to Ensembl genome Started job on | Apr 10 11:10:20 Started mapping on | Apr 10 11:10:20 Finished on | Apr 10 11:15:26 Mapping speed, Million of reads per hour | 294.77 Number of input reads | 25055756 Average input read length | 300 UNIQUE READS: Uniquely mapped reads number | 19851504 Uniquely mapped reads % | 79.23% Average mapped length | 299.35 Number of splices: Total | 17850589 Number of splices: Annotated (sjdb) | 16825710 Number of splices: GT/AG | 17579750 Number of splices: GC/AG | 217224 Number of splices: AT/AC | 6656 Number of splices: Non-canonical | 46959 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 2.77 Insertion rate per base | 0.02% Insertion average length | 2.68 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1418684 % of reads mapped to multiple loci | 5.66% Number of reads mapped to too many loci | 186390 % of reads mapped to too many loci | 0.74% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.29% % of reads unmapped: other | 6.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3785568 3785568 3785568 N_multimapping 1418684 1418684 1418684 N_noFeature 1882712 19227069 2016978 N_ambiguous 612199 3193 123433 UnstrandedReadsAssigned:17356593 PositiveStrandReadsAssigned:621242 NegativeStrandReadsAssigned:17711093 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR7804114 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR7804114-trimmed-pair1.fastq SRR7804114-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,055,756 reads, 18,476,067 reads pseudoaligned [quant] estimated average fragment length: 277.083 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,086 rounds 52973 SRR7804114.ke.tsv 35125 SRR7804114.se.tsv 88098 total ==> SRR7804114.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 660.218 0 0 PNS24247 1044 767.917 47.2566 3.61581 PNS24249 1928 1651.92 122.73 4.36536 PNS24246 1044 767.917 47.2566 3.61581 PNS24248 1044 767.917 47.2566 3.61581 PNS24244 1471 1194.92 88.5001 4.35175 PNS24243 293 77.5532 0 0 KQK14069 1603 1326.92 3068.06 135.855 KQK14071 474 214.728 26.0402 7.12549 ==> SRR7804114.se.tsv <== BRADI_1g14170v3 3102 BRADI_1g53295v3 768 BRADI_1g59795v3 288 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 209 BRADI_1g74790v3 437 BRADI_1g09890v3 0 BRADI_1g77505v3 477 BRADI_1g48960v3 0 SRR7804114 completed mapping pipeline successfully