Starting /dee2/code/volunteer_pipeline.sh SRR7865991 current disk space = 1523527946240 free memory = 1411155736 SRR7865991 SRAfilesize 004100e4553b693df7c81074b25be8cc SRR7865991.sra SRR7865991.sra file validated SRR7865991 is single end SRR7865991 is conventional basespace SRR7865991 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR7865991_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.902 34.0 33.0 34.0 31.0 34.0 2 32.69875 34.0 33.0 34.0 31.0 34.0 3 33.19775 34.0 34.0 34.0 31.0 34.0 4 36.54975 37.0 37.0 37.0 35.0 37.0 5 36.65 37.0 37.0 37.0 35.0 37.0 6 36.6895 37.0 37.0 37.0 37.0 37.0 7 36.7015 37.0 37.0 37.0 37.0 37.0 8 36.69025 37.0 37.0 37.0 37.0 37.0 9 38.57575 39.0 39.0 39.0 38.0 39.0 10-14 38.8025 39.4 39.2 39.4 37.8 39.4 15-19 40.0472 41.0 40.0 41.0 38.4 41.0 20-24 39.8481 41.0 40.0 41.0 38.0 41.0 25-29 39.5822 41.0 40.0 41.0 37.2 41.0 30-34 39.1539 41.0 39.0 41.0 35.4 41.0 35-39 38.902649999999994 41.0 38.6 41.0 35.0 41.0 40-44 38.37725 40.2 37.4 41.0 34.8 41.0 45-49 37.7998 40.0 35.2 41.0 33.6 41.0 50-54 37.01575 39.2 35.0 40.8 32.8 41.0 55-59 36.408950000000004 37.6 35.0 40.6 32.2 41.0 60-64 35.908699999999996 36.4 35.0 39.8 32.0 41.0 65-69 35.38475 35.0 35.0 38.8 32.2 41.0 70-74 34.5012 35.0 34.8 36.8 31.0 39.4 75-79 33.6166 35.0 33.8 35.8 29.8 37.6 80-84 33.10635 35.0 33.8 35.0 29.2 36.4 85-89 32.410000000000004 35.0 33.0 35.0 27.8 35.6 90-94 31.95 35.0 33.0 35.0 25.6 35.0 95-99 31.5466 35.0 32.4 35.0 24.4 35.0 100-104 31.18305 34.8 31.6 35.0 23.4 35.0 105-109 30.72665 34.0 31.0 35.0 21.8 35.0 110-114 30.03315 34.0 30.2 35.0 18.4 35.0 115-119 29.44085 34.0 29.4 35.0 14.0 35.0 120-124 28.55605 33.4 27.8 35.0 4.6 35.0 125-129 27.5745 33.0 25.8 35.0 2.0 35.0 130-134 26.4985 32.4 24.2 34.8 2.0 35.0 135-139 25.20945 31.4 19.6 34.0 2.0 35.0 140-144 23.87855 31.0 10.4 34.0 2.0 35.0 145-149 21.584049999999998 30.2 2.0 34.0 2.0 35.0 150 14.5545 15.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 3.0 5 2.0 6 6.0 7 4.0 8 4.0 9 8.0 10 5.0 11 8.0 12 7.0 13 10.0 14 7.0 15 10.0 16 10.0 17 13.0 18 19.0 19 15.0 20 23.0 21 27.0 22 25.0 23 42.0 24 42.0 25 49.0 26 74.0 27 80.0 28 113.0 29 101.0 30 119.0 31 158.0 32 221.0 33 309.0 34 418.0 35 667.0 36 933.0 37 462.0 38 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.157620041753656 15.187891440501044 12.03027139874739 44.62421711899791 2 33.25 17.75 20.825 28.175 3 24.25 18.375 19.475 37.9 4 28.225 19.575 19.400000000000002 32.800000000000004 5 30.475 23.7 20.825 25.0 6 28.025 26.75 19.25 25.974999999999998 7 22.0 23.275000000000002 30.349999999999998 24.375 8 24.625 21.625 26.375 27.375 9 24.55 21.025 27.400000000000002 27.025 10-14 26.32 24.595 22.795 26.290000000000003 15-19 26.346586646661663 24.081020255063766 22.99574893723431 26.576644161040257 20-24 26.090000000000003 24.12 22.625 27.165 25-29 26.805 23.575 22.45 27.169999999999998 30-34 26.8 23.27 22.58 27.35 35-39 27.29 23.815 22.205 26.69 40-44 26.52 23.525 22.855 27.1 45-49 27.33 23.669999999999998 22.259999999999998 26.740000000000002 50-54 27.560000000000002 23.055 22.040000000000003 27.345000000000002 55-59 27.32 22.805 22.24 27.634999999999998 60-64 26.845000000000002 22.97 22.825 27.36 65-69 26.729999999999997 22.985 23.080000000000002 27.205000000000002 70-74 27.1 22.97 22.830000000000002 27.1 75-79 27.115000000000002 22.869999999999997 22.455 27.560000000000002 80-84 27.36 23.285 22.445 26.91 85-89 27.644999999999996 23.05 22.264999999999997 27.04 90-94 27.894999999999996 22.695 22.41 27.0 95-99 27.615000000000002 22.97 22.105 27.310000000000002 100-104 27.665 22.71 22.134999999999998 27.49 105-109 27.85 22.759999999999998 22.86 26.529999999999998 110-114 27.805000000000003 22.400000000000002 22.509999999999998 27.284999999999997 115-119 27.145000000000003 22.775000000000002 22.005 28.075 120-124 27.715 22.134999999999998 22.555 27.595 125-129 27.985 22.400000000000002 22.295 27.32 130-134 28.27 21.465 22.665 27.6 135-139 27.705000000000002 22.605 22.845 26.845000000000002 140-144 28.110000000000003 22.34 21.759999999999998 27.79 145-149 28.7 22.445 21.18 27.675 150 28.7 22.45 19.675 29.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 0.5 26 0.0 27 1.5 28 2.5 29 2.5 30 1.5 31 1.0 32 4.5 33 12.5 34 16.5 35 18.5 36 33.0 37 45.5 38 55.0 39 73.0 40 87.0 41 103.5 42 111.5 43 119.0 44 139.0 45 139.0 46 143.0 47 157.5 48 156.5 49 140.0 50 141.0 51 139.5 52 110.5 53 101.5 54 113.5 55 118.0 56 108.0 57 83.5 58 67.5 59 83.0 60 95.0 61 81.5 62 71.0 63 80.0 64 87.5 65 83.5 66 73.0 67 71.5 68 81.5 69 87.0 70 71.0 71 59.5 72 65.0 73 58.0 74 54.5 75 49.0 76 36.5 77 31.0 78 25.0 79 19.5 80 19.5 81 16.5 82 9.5 83 5.5 84 6.5 85 7.0 86 4.0 87 3.0 88 3.0 89 4.5 90 4.5 91 1.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.025 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.775 #Duplication Level Percentage of deduplicated Percentage of total 1 99.77449260836883 99.55000000000001 2 0.22550739163117012 0.44999999999999996 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.07500000000000001 0.0 0.0 0.0 0.0 112-113 0.1 0.0 0.0 0.0 0.0 114-115 0.1 0.0 0.0 0.0 0.0 116-117 0.15 0.0 0.0 0.0 0.0 118-119 0.175 0.0 0.0 0.0 0.0 120-121 0.2 0.0 0.0 0.0 0.0 122-123 0.2 0.0 0.0 0.0 0.0 124-125 0.225 0.0 0.0 0.0 0.0 126-127 0.3125 0.0 0.0 0.0 0.0 128-129 0.42500000000000004 0.0 0.0 0.0 0.0 130-131 0.7 0.0 0.0 0.0 0.0 132-133 0.9125 0.0 0.0 0.0 0.0 134-135 1.1875 0.0 0.0 0.0 0.0 136-137 1.6375000000000002 0.0 0.0 0.0 0.0 138 1.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215704 spots for SRR7865991.sra Written 2215704 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra Read 2215700 spots for SRR7865991.sra Written 2215700 spots for SRR7865991.sra SRR ids: ['SRR7865991.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_b2xjelju SRR7865991.sra spots: 44314004 blocks: [[1, 2215700], [2215701, 4431400], [4431401, 6647100], [6647101, 8862800], [8862801, 11078500], [11078501, 13294200], [13294201, 15509900], [15509901, 17725600], [17725601, 19941300], [19941301, 22157000], [22157001, 24372700], [24372701, 26588400], [26588401, 28804100], [28804101, 31019800], [31019801, 33235500], [33235501, 35451200], [35451201, 37666900], [37666901, 39882600], [39882601, 42098300], [42098301, 44314004]] SRR7865991 file size 16305269 SRR7865991 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR7865991 SRR7865991_1.fastq Input file: SRR7865991_1.fastq trimmed: SRR7865991-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Dec 10 01:27:19 2024 >> started Tue Dec 10 01:28:07 2024 >> done (47.454s) 44314004 reads processed; of these: 45688 ( 0.10%) short reads filtered out after trimming by size control 32933 ( 0.07%) empty reads filtered out after trimming by size control 44235383 (99.82%) reads available; of these: 24953678 (56.41%) trimmed reads available after processing 19281705 (43.59%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 5535 0.01% 19 5714 0.01% 20 6508 0.01% 21 7033 0.02% 22 7006 0.02% 23 7774 0.02% 24 8307 0.02% 25 8815 0.02% 26 10212 0.02% 27 9765 0.02% 28 10006 0.02% 29 10837 0.02% 30 10915 0.02% 31 11069 0.03% 32 11362 0.03% 33 11435 0.03% 34 11386 0.03% 35 12171 0.03% 36 12479 0.03% 37 12871 0.03% 38 13064 0.03% 39 14002 0.03% 40 14402 0.03% 41 14737 0.03% 42 15530 0.04% 43 15664 0.04% 44 16159 0.04% 45 16733 0.04% 46 16971 0.04% 47 18096 0.04% 48 18132 0.04% 49 18135 0.04% 50 18167 0.04% 51 14610 0.03% 52 15432 0.03% 53 16254 0.04% 54 16761 0.04% 55 16869 0.04% 56 17362 0.04% 57 17365 0.04% 58 17027 0.04% 59 17147 0.04% 60 17966 0.04% 61 18251 0.04% 62 19357 0.04% 63 20080 0.05% 64 21807 0.05% 65 25352 0.06% 66 24081 0.05% 67 24622 0.06% 68 25681 0.06% 69 24487 0.06% 70 25202 0.06% 71 27531 0.06% 72 28283 0.06% 73 29130 0.07% 74 29750 0.07% 75 30134 0.07% 76 26981 0.06% 77 27808 0.06% 78 31206 0.07% 79 32488 0.07% 80 34856 0.08% 81 37206 0.08% 82 38697 0.09% 83 40506 0.09% 84 41682 0.09% 85 43829 0.10% 86 44954 0.10% 87 46693 0.11% 88 48406 0.11% 89 51513 0.12% 90 54268 0.12% 91 56405 0.13% 92 58539 0.13% 93 60822 0.14% 94 63026 0.14% 95 66584 0.15% 96 69450 0.16% 97 72849 0.16% 98 76617 0.17% 99 79694 0.18% 100 83248 0.19% 101 88795 0.20% 102 92237 0.21% 103 96651 0.22% 104 103567 0.23% 105 106820 0.24% 106 111042 0.25% 107 117081 0.26% 108 120734 0.27% 109 127958 0.29% 110 137089 0.31% 111 142642 0.32% 112 151554 0.34% 113 163976 0.37% 114 174035 0.39% 115 188291 0.43% 116 204848 0.46% 117 210130 0.48% 118 208755 0.47% 119 214473 0.48% 120 215766 0.49% 121 224038 0.51% 122 234057 0.53% 123 240645 0.54% 124 253022 0.57% 125 262993 0.59% 126 274492 0.62% 127 285108 0.64% 128 301938 0.68% 129 316054 0.71% 130 330648 0.75% 131 353190 0.80% 132 365944 0.83% 133 388725 0.88% 134 408217 0.92% 135 415780 0.94% 136 433696 0.98% 137 464125 1.05% 138 494240 1.12% 139 531502 1.20% 140 582841 1.32% 141 632391 1.43% 142 699048 1.58% 143 795552 1.80% 144 902689 2.04% 145 1049047 2.37% 146 1261484 2.85% 147 1486797 3.36% 148 1867660 4.22% 149 3891451 8.80% 150 19281705 43.59% 44235383 reads passed initial QC criterion=sequence-density sequence-density=1.02 sequence-density-rank=1 fanout-score=73.05 fanout-score-rank=12 prefix-density=1.57 prefix-fanout=47.3 sequence=AGATCGGAAGAGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=1674.95 fanout-score-rank=1 prefix-density=1.02 prefix-fanout=24.4 sequence=GCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAGT Started job on | Dec 10 01:28:38 Started mapping on | Dec 10 01:28:39 Finished on | Dec 10 01:29:40 Mapping speed, Million of reads per hour | 2610.61 Number of input reads | 44235383 Average input read length | 139 UNIQUE READS: Uniquely mapped reads number | 42056234 Uniquely mapped reads % | 95.07% Average mapped length | 139.15 Number of splices: Total | 17499012 Number of splices: Annotated (sjdb) | 16563112 Number of splices: GT/AG | 17251841 Number of splices: GC/AG | 197651 Number of splices: AT/AC | 14843 Number of splices: Non-canonical | 34677 Mismatch rate per base, % | 0.27% Deletion rate per base | 0.02% Deletion average length | 2.33 Insertion rate per base | 0.01% Insertion average length | 2.09 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 846192 % of reads mapped to multiple loci | 1.91% Number of reads mapped to too many loci | 879775 % of reads mapped to too many loci | 1.99% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.79% % of reads unmapped: other | 0.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1332957 1332957 1332957 N_multimapping 846192 846192 846192 N_noFeature 1040732 21298342 21238718 N_ambiguous 659753 54983 56415 UnstrandedReadsAssigned:40355749 PositiveStrandReadsAssigned:20702909 NegativeStrandReadsAssigned:20761101 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=138 echo kmer=133 SRR7865991 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: SRR7865991-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 44,235,383 reads, 41,584,219 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,183 rounds 52973 SRR7865991.ke.tsv 35125 SRR7865991.se.tsv 88098 total ==> SRR7865991.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 145.605 6.43448 PNS24247 1044 945 14.51 0.567936 PNS24249 1928 1829 558.038 11.2853 PNS24246 1044 945 14.51 0.567936 PNS24248 1044 945 14.51 0.567936 PNS24244 1471 1372 36.8273 0.99284 PNS24243 293 194 15 2.85991 KQK14069 1603 1504 76.7837 1.88836 KQK14071 474 375 16.2163 1.5995 ==> SRR7865991.se.tsv <== BRADI_1g14170v3 93 BRADI_1g53295v3 32 BRADI_1g59795v3 614 BRADI_1g07683v3 0 BRADI_1g00485v3 362 BRADI_1g20270v3 6079 BRADI_1g74790v3 22 BRADI_1g09890v3 0 BRADI_1g77505v3 510 BRADI_1g48960v3 0 SRR7865991 completed mapping pipeline successfully