Starting /dee2/code/volunteer_pipeline.sh SRR8846505 current disk space = 1507963961344 free memory = 1389894588 SRR8846505 SRAfilesize a055c9cde29a42c4436b62ad11eaaf1e SRR8846505.sra SRR8846505.sra file validated SRR8846505 is paired end SRR8846505 is conventional basespace SRR8846505 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846505_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.27475 34.0 33.0 34.0 33.0 34.0 2 33.306 34.0 33.0 34.0 33.0 34.0 3 33.25575 34.0 33.0 34.0 32.0 34.0 4 33.228 34.0 33.0 34.0 32.0 34.0 5 33.2375 34.0 33.0 34.0 33.0 34.0 6 36.9215 38.0 37.0 38.0 35.0 38.0 7 37.2195 38.0 38.0 38.0 36.0 38.0 8 37.19325 38.0 38.0 38.0 36.0 38.0 9 37.3085 38.0 38.0 38.0 37.0 38.0 10-14 37.277100000000004 38.0 38.0 38.0 36.4 38.0 15-19 37.284 38.0 38.0 38.0 36.8 38.0 20-24 37.356300000000005 38.0 38.0 38.0 37.0 38.0 25-29 37.27910000000001 38.0 38.0 38.0 37.0 38.0 30-34 37.19520000000001 38.0 38.0 38.0 36.2 38.0 35-39 37.203149999999994 38.0 38.0 38.0 36.2 38.0 40-44 37.1795 38.0 38.0 38.0 36.0 38.0 45-49 37.128550000000004 38.0 38.0 38.0 36.0 38.0 50-54 36.98864999999999 38.0 38.0 38.0 35.8 38.0 55-59 36.4784 38.0 38.0 38.0 34.8 38.0 60-64 35.56725 38.0 38.0 38.0 33.4 38.0 65-69 36.414500000000004 38.0 38.0 38.0 34.0 38.0 70-74 36.8318 38.0 38.0 38.0 35.0 38.0 75-79 36.736850000000004 38.0 38.0 38.0 35.0 38.0 80-84 36.5637 38.0 38.0 38.0 34.2 38.0 85-89 36.4861 38.0 38.0 38.0 34.0 38.0 90-94 36.42965 38.0 38.0 38.0 34.0 38.0 95-99 36.296049999999994 38.0 38.0 38.0 34.0 38.0 100-104 36.128949999999996 38.0 37.4 38.0 33.2 38.0 105-109 36.0488 38.0 37.0 38.0 33.0 38.0 110-114 35.759 38.0 37.0 38.0 31.4 38.0 115-119 35.70505 38.0 37.0 38.0 31.0 38.0 120-124 35.3941 38.0 36.4 38.0 30.0 38.0 125-129 34.9735 38.0 35.6 38.0 28.2 38.0 130-134 34.80915 38.0 35.6 38.0 27.6 38.0 135-139 34.15525 38.0 34.6 38.0 23.6 38.0 140-144 33.5515 38.0 33.0 38.0 19.6 38.0 145-149 33.0517 38.0 33.0 38.0 16.6 38.0 150-151 29.098 35.5 27.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 1.0 13 1.0 14 2.0 15 2.0 16 3.0 17 1.0 18 3.0 19 4.0 20 5.0 21 9.0 22 10.0 23 12.0 24 15.0 25 17.0 26 29.0 27 36.0 28 24.0 29 45.0 30 49.0 31 71.0 32 81.0 33 126.0 34 229.0 35 327.0 36 621.0 37 2275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.325 8.725 9.950000000000001 59.0 2 17.65 12.45 44.95 24.95 3 15.675 17.95 29.349999999999998 37.025000000000006 4 20.8 25.124999999999996 26.450000000000003 27.625 5 21.885942971485743 30.190095047523762 31.190595297648827 16.73336668334167 6 17.0 35.825 28.849999999999998 18.325 7 13.575000000000001 27.275 44.925 14.224999999999998 8 15.75 24.975 37.85 21.425 9 15.9 23.05 39.95 21.099999999999998 10-14 18.0 33.239999999999995 26.650000000000002 22.11 15-19 18.369184592296147 31.210605302651324 29.289644822411205 21.13056528264132 20-24 18.355 31.415 28.744999999999997 21.485000000000003 25-29 20.255000000000003 30.659999999999997 28.705000000000002 20.380000000000003 30-34 21.47 31.155 26.424999999999997 20.95 35-39 19.900000000000002 32.815 26.87 20.415 40-44 17.349999999999998 30.15 29.294999999999998 23.205000000000002 45-49 18.5 31.165 28.444999999999997 21.89 50-54 19.0 31.31 28.53 21.16 55-59 20.188268637076774 30.117920947416366 26.413280024292728 23.28053039121413 60-64 18.728281728126134 32.82506094082257 27.61267569109486 20.833981639956434 65-69 20.388447217470063 31.845627452953607 25.717017208413 22.04890812116333 70-74 20.94 30.475 26.174999999999997 22.41 75-79 20.525 30.919999999999998 27.750000000000004 20.805 80-84 21.545 30.195 27.065 21.195 85-89 21.3 31.014999999999997 26.724999999999998 20.96 90-94 20.651032551627583 30.466523326166307 27.561378068903448 21.321066053302665 95-99 20.54 31.04 27.355 21.065 100-104 19.064999999999998 30.785 27.435 22.715 105-109 20.72 28.675 28.225 22.38 110-114 19.56 29.755 27.839999999999996 22.845 115-119 18.57 30.84 27.224999999999998 23.365 120-124 17.740000000000002 31.44 26.19 24.63 125-129 20.001000050002503 32.82164108205411 25.171258562928145 22.00610030501525 130-134 21.566078303915194 31.381569078453925 25.37626881344067 21.67608380419021 135-139 21.543231484722707 31.064659698954845 25.303795569335403 22.08831324698705 140-144 21.975 31.509999999999998 26.009999999999998 20.505000000000003 145-149 20.525 30.535 25.915 23.025000000000002 150-151 20.575 30.112499999999997 26.1125 23.200000000000003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 1.5 19 2.5 20 3.0 21 4.5 22 8.0 23 9.5 24 13.5 25 13.5 26 11.5 27 24.5 28 41.0 29 48.0 30 54.0 31 71.0 32 86.0 33 90.0 34 101.0 35 108.5 36 157.5 37 283.0 38 301.0 39 223.0 40 226.0 41 232.0 42 209.5 43 223.0 44 232.5 45 199.5 46 161.5 47 130.0 48 102.0 49 77.0 50 58.5 51 51.5 52 38.0 53 30.5 54 30.0 55 35.5 56 34.5 57 22.5 58 27.0 59 31.5 60 29.0 61 21.0 62 14.5 63 16.0 64 21.5 65 30.0 66 18.5 67 6.0 68 6.5 69 4.5 70 3.0 71 6.0 72 5.5 73 1.5 74 2.5 75 2.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.05 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.05 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.205 60-64 3.595 65-69 0.63 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.005 130-134 0.005 135-139 0.015 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.0 #Duplication Level Percentage of deduplicated Percentage of total 1 87.02702702702703 64.4 2 7.4324324324324325 11.0 3 2.4662162162162162 5.475 4 1.114864864864865 3.3000000000000003 5 0.7770270270270271 2.875 6 0.37162162162162166 1.6500000000000001 7 0.10135135135135136 0.525 8 0.13513513513513514 0.8 9 0.06756756756756757 0.44999999999999996 >10 0.4391891891891892 5.7250000000000005 >50 0.06756756756756757 3.8 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 79 1.975 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 73 1.825 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 34 0.8500000000000001 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 26 0.65 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 22 0.5499999999999999 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 21 0.525 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 18 0.44999999999999996 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 17 0.42500000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 15 0.375 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 14 0.35000000000000003 No Hit CTTTTTTTGATTGTCTGTCAATCAATATTCTAATGGCAATGCAATTTCAT 13 0.325 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 13 0.325 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 13 0.325 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 12 0.3 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 11 0.27499999999999997 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 8 0.2 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 8 0.2 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 7 0.17500000000000002 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 7 0.17500000000000002 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 7 0.17500000000000002 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 6 0.15 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 6 0.15 No Hit CTTTTTCAAACCTGCTCTGCTCCCATTTAAGGAAAAAGAATTTCACGTTC 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit GGGTAAACCACCGCCTCTCAGGCCTCCCCGACGGGTTCTACCATAGAGGC 6 0.15 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 6 0.15 No Hit CACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAAT 6 0.15 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 6 0.15 No Hit CGGTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTTCA 6 0.15 No Hit GTGGTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCG 6 0.15 No Hit CCCTTATCCTCATTACGCCTAGCATTTAGTGGGCTGGATATTTACCTTAT 5 0.125 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit CTACCTTTTTGACAGCCCATCTTTTTGTCTCAGTAGAGTCTTTCAGTGGC 5 0.125 No Hit CTTGGTTTCATACTCCGGGGTGTAGTAAGTCAATCTATAATCTTTAACAC 5 0.125 No Hit CTCCAACTATCGTCCATGTACGATCCATACTAGATCTGACCAACTGCCCA 5 0.125 No Hit CATCTTTTTGTCTCAGTAGAGTCTTTCAGTGGCATGTTTCAGTCCTCTTC 5 0.125 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 5 0.125 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 5 0.125 No Hit CTAGCATTTAGTGGGCTGGATATTTACCTTATCAACTAGCAAATCCATAA 5 0.125 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 5 0.125 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 5 0.125 No Hit CCCTACGATCCAACCAATTGGGAGAGAATCAATAGACTCCTTTTCGGGAG 5 0.125 No Hit CATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATATCTACT 5 0.125 No Hit CCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTCTCTCTAAAA 5 0.125 No Hit CTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAA 5 0.125 No Hit CCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCA 5 0.125 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 5 0.125 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit GTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCC 5 0.125 No Hit CTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.5874999999999999 0.0 0.0 0.0 0.0 96-97 0.8 0.0 0.0 0.0 0.0 98-99 0.9875 0.0 0.0 0.0 0.0 100-101 1.1875 0.0 0.0 0.0 0.0 102-103 1.4125 0.0 0.0 0.0 0.0 104-105 1.725 0.0 0.0 0.0 0.0 106-107 2.1500000000000004 0.0 0.0 0.0 0.0 108-109 2.55 0.0 0.0 0.0 0.0 110-111 2.9625000000000004 0.0 0.0 0.0 0.0 112-113 3.475 0.0 0.0 0.0 0.0 114-115 3.9625000000000004 0.0 0.0 0.0 0.0 116-117 4.6375 0.0 0.0 0.0 0.0 118-119 5.3375 0.0 0.0 0.0 0.0 120-121 6.0625 0.0 0.0 0.0 0.0 122-123 6.675 0.0 0.0 0.0 0.0 124-125 7.425 0.0 0.0 0.0 0.0 126-127 8.162500000000001 0.0 0.0 0.0 0.0 128-129 9.0625 0.0 0.0 0.0 0.0 130-131 10.275 0.0 0.0 0.0 0.0 132-133 11.2875 0.0 0.0 0.0 0.0 134-135 12.1125 0.0 0.0 0.0 0.0 136-137 12.8125 0.0 0.0 0.0 0.0 138-139 13.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACA 10 0.0068768123 144.675 5 ATAACAA 10 0.0068768123 144.675 6 TTGATTG 10 0.0068768123 144.675 7 TAACAAG 10 0.0068768123 144.675 7 CCATATA 10 0.0068768123 144.675 2 CATATAA 10 0.0068768123 144.675 3 CTCGCAA 10 0.0068768123 144.675 1 TTCAAAA 60 0.0045593763 24.112501 7 TATATGT 50 0.0013499795 17.361 20-24 TTATATG 50 0.0013499795 17.361 20-24 TGTTAGC 50 0.0013499795 17.361 25-29 ATATGTT 50 0.0013499795 17.361 20-24 GTTAGCG 50 0.0013499795 17.361 25-29 TTAGCGG 50 0.0013499795 17.361 25-29 AAACCTT 55 0.0025540418 15.782728 35-39 AATTCTT 55 0.0025540418 15.782728 15-19 GCGGAAA 55 0.0025540418 15.782728 30-34 CGGAAAA 55 0.0025540418 15.782728 30-34 TTCTTAT 55 0.0025540418 15.782728 15-19 AAAACCT 55 0.0025540418 15.782728 35-39 >>END_MODULE SRR8846505 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846505_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.721 33.0 33.0 34.0 32.0 34.0 2 32.91675 33.0 33.0 34.0 32.0 34.0 3 32.93475 33.0 33.0 34.0 32.0 34.0 4 33.00975 33.0 33.0 34.0 32.0 34.0 5 32.9495 34.0 33.0 34.0 32.0 34.0 6 37.1545 38.0 38.0 38.0 37.0 38.0 7 37.17425 38.0 38.0 38.0 37.0 38.0 8 37.1855 38.0 38.0 38.0 37.0 38.0 9 37.242 38.0 38.0 38.0 37.0 38.0 10-14 37.11845 38.0 38.0 38.0 36.4 38.0 15-19 37.10415 38.0 38.0 38.0 36.2 38.0 20-24 37.0627 38.0 38.0 38.0 36.4 38.0 25-29 37.057449999999996 38.0 38.0 38.0 36.2 38.0 30-34 37.0685 38.0 38.0 38.0 36.0 38.0 35-39 37.00825 38.0 38.0 38.0 36.0 38.0 40-44 36.99705 38.0 38.0 38.0 36.0 38.0 45-49 37.002449999999996 38.0 38.0 38.0 36.0 38.0 50-54 36.848 38.0 38.0 38.0 35.6 38.0 55-59 36.82255 38.0 38.0 38.0 35.6 38.0 60-64 36.78914999999999 38.0 38.0 38.0 35.4 38.0 65-69 36.75105 38.0 38.0 38.0 35.0 38.0 70-74 36.7836 38.0 38.0 38.0 35.0 38.0 75-79 36.69234999999999 38.0 38.0 38.0 35.0 38.0 80-84 36.52125 38.0 38.0 38.0 34.4 38.0 85-89 36.44685 38.0 38.0 38.0 34.2 38.0 90-94 36.351549999999996 38.0 38.0 38.0 34.0 38.0 95-99 36.256249999999994 38.0 38.0 38.0 34.0 38.0 100-104 36.090149999999994 38.0 37.8 38.0 33.0 38.0 105-109 35.944449999999996 38.0 37.0 38.0 32.8 38.0 110-114 35.82935 38.0 37.0 38.0 31.8 38.0 115-119 35.624199999999995 38.0 37.0 38.0 31.0 38.0 120-124 35.4456 38.0 36.6 38.0 30.6 38.0 125-129 35.0401 38.0 36.0 38.0 28.0 38.0 130-134 34.7783 38.0 35.4 38.0 27.8 38.0 135-139 34.22525 38.0 35.0 38.0 24.0 38.0 140-144 33.673199999999994 38.0 34.8 38.0 20.8 38.0 145-149 32.262950000000004 38.0 33.6 38.0 11.0 38.0 150-151 27.9775 35.0 17.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 2.0 4 2.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 0.0 13 3.0 14 5.0 15 3.0 16 3.0 17 1.0 18 3.0 19 4.0 20 8.0 21 9.0 22 9.0 23 10.0 24 13.0 25 14.0 26 30.0 27 24.0 28 29.0 29 42.0 30 60.0 31 73.0 32 81.0 33 128.0 34 185.0 35 268.0 36 578.0 37 2404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.96693386773547 20.56613226452906 16.708416833667332 41.758517034068134 2 20.225281602002504 21.952440550688358 43.529411764705884 14.292866082603254 3 16.016016016016017 25.875875875875877 37.61261261261261 20.495495495495494 4 21.391043282461847 31.098323742807104 28.121090818113586 19.389542156617463 5 23.43515272909364 33.074611917876815 29.494241362043066 13.99599399098648 6 18.582519408965688 35.662409216128225 31.05434510393188 14.700726270974204 7 17.088449010273116 19.99498872463042 46.40440992232523 16.512152342771238 8 18.837675350701403 22.394789579158317 37.04909819639279 21.718436873747496 9 21.317635270541082 19.864729458917836 39.30360721442886 19.514028056112224 10-14 22.28514156852919 27.877724880982207 30.633926334252067 19.20320721623653 15-19 22.362023159055592 27.104115494511007 31.610607047972326 18.923254298461075 20-24 22.262865160094204 26.807636418299342 31.978754321791854 18.9507440998146 25-29 21.92432974191932 26.29917313956402 32.232523177148586 19.54397394136808 30-34 23.435386080072153 26.111138948739793 31.307310718043794 19.14616425314426 35-39 22.29905792744037 26.844056925235517 31.138504710362795 19.718380436961315 40-44 22.74867274366423 26.720424722027445 30.982670539917862 19.548231994390463 45-49 21.76809416478838 28.635111445028798 30.588529927372903 19.00826446280992 50-54 20.975658619653412 28.09275768806972 30.982670539917862 19.94891315235901 55-59 20.607092766980564 28.005409737527547 30.249449008214786 21.138048487277096 60-64 21.293004156442485 27.788071510841807 30.957984876558665 19.960939456157043 65-69 21.18542250700841 27.452943532238688 31.43772527032439 19.92390869042851 70-74 22.141569883860633 27.197637164597516 30.992190628754507 19.668602322787347 75-79 22.276138069034516 26.908454227113555 31.41570785392696 19.39969984992496 80-84 22.75979386601291 27.202681743133038 31.145244408865764 18.892279981988292 85-89 22.339626420952477 27.87821122740247 30.18178176173068 19.60038058991437 90-94 22.335737179487182 27.774439102564102 29.872796474358974 20.017027243589745 95-99 21.65614667868951 28.138463079851718 30.402765253982565 19.802624987476207 100-104 22.858431018935978 28.534215008516178 29.92185151788398 18.68550245466386 105-109 23.373240494915592 26.774532885838802 30.386214496819115 19.46601212242649 110-114 22.78601482668804 27.629733520336607 30.094169505109196 19.490082147866158 115-119 22.458178904137032 28.378243013122308 29.950916558148855 19.212661524591805 120-124 23.522636217948715 27.994791666666668 28.670873397435898 19.811698717948715 125-129 24.2017816034431 27.935141627464716 28.961064958462618 18.902011810629567 130-134 23.214017521902377 28.816020025031293 29.036295369211512 18.933667083854818 135-139 24.426639959939912 28.5628442663996 28.95343014521783 18.057085628442664 140-144 25.30289376189046 29.09782717532793 27.64093321317713 17.958345849604484 145-149 25.811298076923077 27.93469551282051 28.175080128205128 18.078926282051285 150-151 26.307230422817113 28.383787840880657 28.096072054040533 17.212909682261696 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 0.5 10 0.5 11 0.0 12 0.5 13 1.0 14 0.5 15 0.0 16 0.5 17 1.0 18 1.5 19 1.0 20 1.0 21 4.5 22 9.5 23 11.5 24 15.0 25 17.5 26 23.5 27 34.0 28 43.0 29 51.0 30 56.5 31 71.5 32 77.0 33 87.5 34 134.0 35 173.0 36 178.0 37 199.5 38 221.5 39 214.0 40 217.0 41 233.0 42 234.5 43 231.0 44 219.0 45 179.5 46 144.5 47 117.5 48 102.5 49 84.0 50 77.0 51 73.0 52 45.0 53 35.5 54 34.0 55 31.0 56 26.0 57 14.5 58 20.0 59 31.5 60 28.5 61 25.5 62 22.5 63 28.5 64 30.5 65 22.5 66 13.5 67 7.0 68 9.0 69 7.5 70 4.5 71 3.0 72 2.0 73 1.5 74 3.0 75 2.5 76 2.0 77 1.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.125 3 0.1 4 0.075 5 0.15 6 0.17500000000000002 7 0.22499999999999998 8 0.2 9 0.2 10-14 0.22499999999999998 15-19 0.255 20-24 0.215 25-29 0.22499999999999998 30-34 0.215 35-39 0.22 40-44 0.16999999999999998 45-49 0.17500000000000002 50-54 0.16999999999999998 55-59 0.18 60-64 0.155 65-69 0.12 70-74 0.12 75-79 0.05 80-84 0.065 85-89 0.155 90-94 0.16 95-99 0.19 100-104 0.19 105-109 0.185 110-114 0.18 115-119 0.16999999999999998 120-124 0.16 125-129 0.09 130-134 0.125 135-139 0.15 140-144 0.13 145-149 0.16 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.9 #Duplication Level Percentage of deduplicated Percentage of total 1 86.72595520421608 65.825 2 7.246376811594203 11.0 3 2.635046113306983 6.0 4 1.0540184453227932 3.2 5 0.7575757575757576 2.875 6 0.2635046113306983 1.2 7 0.4940711462450593 2.625 8 0.1976284584980237 1.2 9 0.1976284584980237 1.35 >10 0.4281949934123847 4.725 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 24 0.6 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 23 0.575 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 19 0.475 No Hit CTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGT 17 0.42500000000000004 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 15 0.375 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 14 0.35000000000000003 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 12 0.3 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 11 0.27499999999999997 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 11 0.27499999999999997 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 11 0.27499999999999997 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 11 0.27499999999999997 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 11 0.27499999999999997 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 10 0.25 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 9 0.22499999999999998 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 9 0.22499999999999998 No Hit CTTGGTATGGAAGTAATGCACGAACGTAATGCTCACAACTTCCCTCTAGA 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit TATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAG 9 0.22499999999999998 No Hit CCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATA 9 0.22499999999999998 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 8 0.2 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit GTCTAATTGTCCAGGCGGTAATGATAGTATCTTGTACCTGAACCGGTGGC 7 0.17500000000000002 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 7 0.17500000000000002 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 7 0.17500000000000002 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit GCTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGG 7 0.17500000000000002 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 7 0.17500000000000002 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 7 0.17500000000000002 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 7 0.17500000000000002 No Hit CCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGG 7 0.17500000000000002 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 7 0.17500000000000002 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 7 0.17500000000000002 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 7 0.17500000000000002 No Hit CTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAAC 7 0.17500000000000002 No Hit GTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACTGAATTTAAAATG 7 0.17500000000000002 No Hit CTCGTTTACACGTGCGCCAATGCTTTTCAAAGGAGCTTATTATGCAATGA 6 0.15 No Hit ATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGT 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 6 0.15 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 6 0.15 No Hit CTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAACCATTTATGCG 6 0.15 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 6 0.15 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 6 0.15 No Hit TGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAA 5 0.125 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 5 0.125 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 5 0.125 No Hit GCTCAGTTCGGTAGAGCAACTCGTTTACACGTGCGCCAATGCTTTTCAAA 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 5 0.125 No Hit CGTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGAC 5 0.125 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit CAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGC 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit CAAAAAATTACGGTAGAGCGTGTTATGAGTGTCTACGTGGTGGACTTGAT 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.5874999999999999 0.0 0.0 0.0 0.0 96-97 0.7749999999999999 0.0 0.0 0.0 0.0 98-99 0.9875 0.0 0.0 0.0 0.0 100-101 1.1875 0.0 0.0 0.0 0.0 102-103 1.4249999999999998 0.0 0.0 0.0 0.0 104-105 1.7374999999999998 0.0 0.0 0.0 0.0 106-107 2.175 0.0 0.0 0.0 0.0 108-109 2.55 0.0 0.0 0.0 0.0 110-111 2.9625000000000004 0.0 0.0 0.0 0.0 112-113 3.475 0.0 0.0 0.0 0.0 114-115 3.9625000000000004 0.0 0.0 0.0 0.0 116-117 4.6375 0.0 0.0 0.0 0.0 118-119 5.3375 0.0 0.0 0.0 0.0 120-121 6.112500000000001 0.0 0.0 0.0 0.0 122-123 6.7125 0.0 0.0 0.0 0.0 124-125 7.45 0.0 0.0 0.0 0.0 126-127 8.225 0.0 0.0 0.0 0.0 128-129 9.125 0.0 0.0 0.0 0.0 130-131 10.3125 0.0 0.0 0.0 0.0 132-133 11.325 0.0 0.0 0.0 0.0 134-135 12.15 0.0 0.0 0.0 0.0 136-137 12.825 0.0 0.0 0.0 0.0 138-139 13.7625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533650 spots for SRR8846505.sra Written 1533650 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra Read 1533637 spots for SRR8846505.sra Written 1533637 spots for SRR8846505.sra SRR ids: ['SRR8846505.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0ej7uu87 SRR8846505.sra spots: 30672753 blocks: [[1, 1533637], [1533638, 3067274], [3067275, 4600911], [4600912, 6134548], [6134549, 7668185], [7668186, 9201822], [9201823, 10735459], [10735460, 12269096], [12269097, 13802733], [13802734, 15336370], [15336371, 16870007], [16870008, 18403644], [18403645, 19937281], [19937282, 21470918], [21470919, 23004555], [23004556, 24538192], [24538193, 26071829], [26071830, 27605466], [27605467, 29139103], [29139104, 30672753]] SRR8846505 file size 10372289 SRR8846505 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846505 SRR8846505_1.fastq SRR8846505_2.fastq Input file: SRR8846505_1.fastq Paired file: SRR8846505_2.fastq trimmed: SRR8846505-trimmed-pair1.fastq, SRR8846505-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 02:50:21 2024 >> started Mon Dec 9 02:54:08 2024 >> done (226.842s) 30672753 read pairs processed; of these: 10435 ( 0.03%) short read pairs filtered out after trimming by size control 88004 ( 0.29%) empty read pairs filtered out after trimming by size control 30574314 (99.68%) read pairs available; of these: 14272332 (46.68%) trimmed read pairs available after processing 16301982 (53.32%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 18 0.00% 19 7 0.00% 20 5 0.00% 21 6 0.00% 22 6 0.00% 23 3 0.00% 24 6 0.00% 25 9 0.00% 26 7 0.00% 27 39 0.00% 28 11 0.00% 29 7 0.00% 30 14 0.00% 31 11 0.00% 32 11 0.00% 33 11 0.00% 34 15 0.00% 35 11 0.00% 36 17 0.00% 37 11 0.00% 38 22 0.00% 39 27 0.00% 40 24 0.00% 41 19 0.00% 42 33 0.00% 43 44 0.00% 44 39 0.00% 45 53 0.00% 46 58 0.00% 47 54 0.00% 48 67 0.00% 49 87 0.00% 50 113 0.00% 51 103 0.00% 52 118 0.00% 53 118 0.00% 54 137 0.00% 55 191 0.00% 56 185 0.00% 57 223 0.00% 58 269 0.00% 59 322 0.00% 60 334 0.00% 61 367 0.00% 62 482 0.00% 63 518 0.00% 64 637 0.00% 65 687 0.00% 66 781 0.00% 67 836 0.00% 68 964 0.00% 69 1111 0.00% 70 1212 0.00% 71 1403 0.00% 72 1678 0.01% 73 1937 0.01% 74 2286 0.01% 75 2748 0.01% 76 2942 0.01% 77 3309 0.01% 78 3515 0.01% 79 3832 0.01% 80 4453 0.01% 81 5283 0.02% 82 6127 0.02% 83 6888 0.02% 84 7697 0.03% 85 9637 0.03% 86 10328 0.03% 87 11422 0.04% 88 12218 0.04% 89 13057 0.04% 90 15740 0.05% 91 16488 0.05% 92 19272 0.06% 93 21494 0.07% 94 24385 0.08% 95 26390 0.09% 96 27537 0.09% 97 28890 0.09% 98 32849 0.11% 99 36041 0.12% 100 38137 0.12% 101 41800 0.14% 102 45809 0.15% 103 48562 0.16% 104 53601 0.18% 105 61211 0.20% 106 65486 0.21% 107 67219 0.22% 108 68573 0.22% 109 81164 0.27% 110 79267 0.26% 111 86403 0.28% 112 86608 0.28% 113 83051 0.27% 114 87791 0.29% 115 92921 0.30% 116 101840 0.33% 117 109359 0.36% 118 106806 0.35% 119 117626 0.38% 120 119869 0.39% 121 122055 0.40% 122 125698 0.41% 123 125762 0.41% 124 135249 0.44% 125 152802 0.50% 126 147846 0.48% 127 155461 0.51% 128 163972 0.54% 129 175749 0.57% 130 167162 0.55% 131 182735 0.60% 132 170228 0.56% 133 172043 0.56% 134 179892 0.59% 135 179551 0.59% 136 193203 0.63% 137 192624 0.63% 138 212243 0.69% 139 217561 0.71% 140 223455 0.73% 141 250115 0.82% 142 234447 0.77% 143 260859 0.85% 144 280763 0.92% 145 323860 1.06% 146 336965 1.10% 147 412838 1.35% 148 556793 1.82% 149 976513 3.19% 150 5230479 17.11% 151 16301982 53.32% 30574314 reads passed initial QC criterion=sequence-density sequence-density=1.71 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=36 prefix-density=1.71 prefix-fanout=1.9 sequence=TGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=1134.66 fanout-score-rank=1 prefix-density=8.78 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.07 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=31 prefix-density=1.08 prefix-fanout=2.0 sequence=GCGTGAGGCTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=192.04 fanout-score-rank=1 prefix-density=0.49 prefix-fanout=4.3 sequence=AAAGGAAAATGGGGATATGGCGAAATCGGTAGACGCTACGGACTTGATTGTATTGAGCCTTAGTATGGAAACCTGCTAAGTGTTAACTTCCAAATTCAGAGAAACCCTGGAATTAAAAAAGGGCAATCCTGAGCCAAATCCGTGTTTTGAGAAAACAAGGGGTTCTCGAACTAGAATCCAAAGGAAAAGGATAGGTGCAGAGACTCAATGGAAGCTGTTCTAACGAATCGAGTTAATTTATTTAGGTTGTTTTGG SRR8846505 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 02:58:13 Started mapping on | Dec 09 02:58:13 Finished on | Dec 09 03:22:48 Mapping speed, Million of reads per hour | 74.62 Number of input reads | 30574314 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 16830471 Uniquely mapped reads % | 55.05% Average mapped length | 291.53 Number of splices: Total | 3379985 Number of splices: Annotated (sjdb) | 3017434 Number of splices: GT/AG | 3245367 Number of splices: GC/AG | 38449 Number of splices: AT/AC | 15699 Number of splices: Non-canonical | 80470 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 1.74 Insertion rate per base | 0.02% Insertion average length | 1.74 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12616513 % of reads mapped to multiple loci | 41.27% Number of reads mapped to too many loci | 6522 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.25% % of reads unmapped: other | 0.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1132916 1132916 1132916 N_multimapping 12616513 12616513 12616513 N_noFeature 4475064 15933323 4844788 N_ambiguous 1099422 29469 583531 UnstrandedReadsAssigned:11255985 PositiveStrandReadsAssigned:867679 NegativeStrandReadsAssigned:11402152 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=143 echo kmer=139 SRR8846505 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846505-trimmed-pair1.fastq SRR8846505-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,574,314 reads, 18,068,386 reads pseudoaligned [quant] estimated average fragment length: 204.944 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,023 rounds 52973 SRR8846505.ke.tsv 35125 SRR8846505.se.tsv 88098 total ==> SRR8846505.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 732.628 0 0 PNS24247 1044 840.056 8.19439 0.582623 PNS24249 1928 1724.06 20.2305 0.700866 PNS24246 1044 840.056 8.19439 0.582623 PNS24248 1044 840.056 8.19439 0.582623 PNS24244 1471 1267.06 28.1863 1.32868 PNS24243 293 113.317 0 0 KQK14069 1603 1399.06 1454.63 62.1007 KQK14071 474 275.558 22.7732 4.93617 ==> SRR8846505.se.tsv <== BRADI_1g14170v3 1739 BRADI_1g53295v3 25 BRADI_1g59795v3 25 BRADI_1g07683v3 0 BRADI_1g00485v3 7 BRADI_1g20270v3 275 BRADI_1g74790v3 16 BRADI_1g09890v3 0 BRADI_1g77505v3 23 BRADI_1g48960v3 0 SRR8846505 completed mapping pipeline successfully