Starting /dee2/code/volunteer_pipeline.sh SRR8846509 current disk space = 1506744619008 free memory = 1356250936 SRR8846509 SRAfilesize 92db02431144b304581ebb0e417f9cf5 SRR8846509.sra SRR8846509.sra file validated SRR8846509 is paired end SRR8846509 is conventional basespace SRR8846509 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846509_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.31575 34.0 33.0 34.0 33.0 34.0 2 33.32575 34.0 33.0 34.0 33.0 34.0 3 33.29125 34.0 33.0 34.0 33.0 34.0 4 33.2615 34.0 33.0 34.0 33.0 34.0 5 33.2585 34.0 33.0 34.0 33.0 34.0 6 36.7325 38.0 37.0 38.0 34.0 38.0 7 37.06825 38.0 38.0 38.0 36.0 38.0 8 37.17425 38.0 38.0 38.0 36.0 38.0 9 37.21325 38.0 38.0 38.0 36.0 38.0 10-14 37.268950000000004 38.0 38.0 38.0 36.8 38.0 15-19 37.264199999999995 38.0 38.0 38.0 36.8 38.0 20-24 37.342200000000005 38.0 38.0 38.0 37.0 38.0 25-29 37.2534 38.0 38.0 38.0 36.8 38.0 30-34 37.23455 38.0 38.0 38.0 36.4 38.0 35-39 37.1789 38.0 38.0 38.0 36.2 38.0 40-44 37.2197 38.0 38.0 38.0 36.4 38.0 45-49 37.15545 38.0 38.0 38.0 36.0 38.0 50-54 37.0343 38.0 38.0 38.0 36.0 38.0 55-59 36.62044999999999 38.0 38.0 38.0 35.2 38.0 60-64 35.6374 38.0 38.0 38.0 33.6 38.0 65-69 36.48245 38.0 38.0 38.0 33.8 38.0 70-74 36.9226 38.0 38.0 38.0 35.4 38.0 75-79 36.8231 38.0 38.0 38.0 35.2 38.0 80-84 36.7249 38.0 38.0 38.0 34.8 38.0 85-89 36.5909 38.0 38.0 38.0 34.0 38.0 90-94 36.51344999999999 38.0 38.0 38.0 34.0 38.0 95-99 36.3981 38.0 38.0 38.0 34.0 38.0 100-104 36.176700000000004 38.0 37.4 38.0 33.4 38.0 105-109 36.10525 38.0 37.2 38.0 33.2 38.0 110-114 35.93535000000001 38.0 37.0 38.0 32.8 38.0 115-119 35.810500000000005 38.0 37.0 38.0 31.6 38.0 120-124 35.647349999999996 38.0 36.4 38.0 31.0 38.0 125-129 35.12584999999999 38.0 35.6 38.0 28.6 38.0 130-134 34.888 38.0 35.4 38.0 27.8 38.0 135-139 34.40265 38.0 34.8 38.0 25.6 38.0 140-144 33.92295 38.0 33.6 38.0 23.0 38.0 145-149 33.46305 38.0 33.0 38.0 21.8 38.0 150-151 29.2945 35.5 27.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 5.0 19 7.0 20 4.0 21 6.0 22 8.0 23 13.0 24 13.0 25 13.0 26 21.0 27 23.0 28 37.0 29 51.0 30 49.0 31 81.0 32 76.0 33 140.0 34 219.0 35 305.0 36 595.0 37 2333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.35 9.75 10.424999999999999 57.475 2 16.45 12.950000000000001 46.425 24.175 3 15.9 17.775 31.525 34.8 4 20.375 24.349999999999998 28.925 26.35 5 21.525 30.25 28.675 19.55 6 18.224999999999998 34.599999999999994 28.525 18.65 7 12.425 27.925 45.35 14.299999999999999 8 15.9 25.424999999999997 39.4 19.275000000000002 9 15.174999999999999 22.8 39.225 22.8 10-14 17.375 34.695 26.51 21.42 15-19 18.618723744748948 32.13142628525706 28.970794158831765 20.279055811162234 20-24 18.39 30.785 29.270000000000003 21.555 25-29 20.82 31.71 27.800000000000004 19.67 30-34 22.205 31.919999999999998 24.83 21.044999999999998 35-39 19.86 33.815 25.44 20.885 40-44 17.88 30.349999999999998 28.945 22.825 45-49 19.005 30.764999999999997 28.044999999999998 22.185 50-54 19.62 31.840000000000003 27.99 20.549999999999997 55-59 21.45204509833662 30.14813691288741 24.758582334799534 23.641235653976437 60-64 18.440047774835126 32.79326997974763 27.216077270602895 21.550604974814352 65-69 20.880281690140844 31.976861167002014 25.2364185110664 21.906438631790746 70-74 22.875 30.185000000000002 24.169999999999998 22.770000000000003 75-79 20.61 31.15 27.145000000000003 21.095 80-84 22.605 30.64 25.705 21.05 85-89 22.145 30.625000000000004 26.240000000000002 20.990000000000002 90-94 21.875 31.009999999999998 27.025 20.09 95-99 21.154999999999998 31.295 26.395000000000003 21.154999999999998 100-104 19.470000000000002 30.605 27.165 22.759999999999998 105-109 21.215 27.515 27.779999999999998 23.49 110-114 19.555 29.21 28.000000000000004 23.235 115-119 18.95 30.39 27.48 23.18 120-124 17.16 32.365 25.44 25.035 125-129 20.685000000000002 32.445 24.19 22.68 130-134 22.435 31.97 24.415 21.18 135-139 22.931146557327867 30.32151607580379 24.046202310115504 22.70113505675284 140-144 21.625 31.39 26.855 20.13 145-149 21.075 31.66 24.975 22.29 150-151 19.875 31.05 24.325 24.75 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.5 19 1.5 20 2.0 21 3.0 22 5.0 23 8.5 24 9.5 25 16.0 26 19.5 27 19.0 28 32.5 29 40.0 30 52.5 31 61.0 32 63.0 33 70.0 34 82.0 35 91.5 36 146.0 37 342.5 38 381.5 39 231.0 40 220.0 41 236.0 42 209.0 43 218.5 44 214.0 45 199.0 46 153.5 47 103.5 48 101.0 49 85.0 50 61.5 51 46.0 52 38.0 53 31.5 54 24.0 55 27.5 56 26.5 57 20.5 58 27.0 59 39.5 60 36.5 61 27.5 62 22.0 63 17.0 64 26.0 65 40.5 66 25.0 67 9.0 68 10.5 69 6.0 70 4.0 71 3.5 72 2.5 73 2.5 74 2.0 75 1.5 76 1.5 77 1.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.02 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.105 60-64 3.7150000000000003 65-69 0.6 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.25 #Duplication Level Percentage of deduplicated Percentage of total 1 86.81318681318682 59.25 2 7.069597069597069 9.65 3 2.5274725274725274 5.175 4 0.8791208791208791 2.4 5 0.5860805860805861 2.0 6 0.43956043956043955 1.7999999999999998 7 0.5128205128205128 2.45 8 0.14652014652014653 0.8 9 0.10989010989010989 0.675 >10 0.8424908424908425 9.65 >50 0.0 0.0 >100 0.07326007326007326 6.15 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 125 3.125 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 121 3.025 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 42 1.05 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 36 0.8999999999999999 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 30 0.75 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 25 0.625 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 22 0.5499999999999999 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 21 0.525 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 18 0.44999999999999996 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 18 0.44999999999999996 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 16 0.4 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 14 0.35000000000000003 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 13 0.325 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 13 0.325 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 13 0.325 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 12 0.3 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 12 0.3 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 11 0.27499999999999997 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 10 0.25 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 10 0.25 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 10 0.25 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 10 0.25 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 10 0.25 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 10 0.25 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 10 0.25 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 9 0.22499999999999998 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 9 0.22499999999999998 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 9 0.22499999999999998 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 8 0.2 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 8 0.2 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 8 0.2 No Hit GTGGTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCG 8 0.2 No Hit CAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGAT 7 0.17500000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 7 0.17500000000000002 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 7 0.17500000000000002 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 7 0.17500000000000002 No Hit CTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACTGTCT 7 0.17500000000000002 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 7 0.17500000000000002 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 7 0.17500000000000002 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 7 0.17500000000000002 No Hit CGGTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTTCA 7 0.17500000000000002 No Hit CCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCG 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CTTTGGAGTAGGCTATGAGACCCAAGCGGGCCAGGAATGCAGCGGCCCGT 7 0.17500000000000002 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 7 0.17500000000000002 No Hit CTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAG 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit CTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAA 6 0.15 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 6 0.15 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit CTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTT 6 0.15 No Hit CTTTTTTTGATTGTCTGTCAATCAATATTCTAATGGCAATGCAATTTCAT 5 0.125 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 5 0.125 No Hit CAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGG 5 0.125 No Hit CCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGACT 5 0.125 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 5 0.125 No Hit CTCCCGTGCTTCCAGACATGCTGAGCTCCCCAAATTTTTGTACATTCAAA 5 0.125 No Hit CACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAG 5 0.125 No Hit TTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAG 5 0.125 No Hit CTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAA 5 0.125 No Hit CTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGAT 5 0.125 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 5 0.125 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit CTCTGGGCCACCCTGCGTCAGCCGGAGATGGGCAGGAACGATCCTCTACA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.175 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.21250000000000002 0.0 0.0 0.0 0.0 94-95 0.275 0.0 0.0 0.0 0.0 96-97 0.35 0.0 0.0 0.0 0.0 98-99 0.475 0.0 0.0 0.0 0.0 100-101 0.675 0.0 0.0 0.0 0.0 102-103 0.9125 0.0 0.0 0.0 0.0 104-105 1.1124999999999998 0.0 0.0 0.0 0.0 106-107 1.2999999999999998 0.0 0.0 0.0 0.0 108-109 1.5375 0.0 0.0 0.0 0.0 110-111 1.825 0.0 0.0 0.0 0.0 112-113 2.05 0.0 0.0 0.0 0.0 114-115 2.2875 0.0 0.0 0.0 0.0 116-117 2.65 0.0 0.0 0.0 0.0 118-119 3.05 0.0 0.0 0.0 0.0125 120-121 3.5 0.0 0.0 0.0 0.025 122-123 3.8625 0.0 0.0 0.0 0.025 124-125 4.4 0.0 0.0 0.0 0.025 126-127 5.0 0.0 0.0 0.0 0.025 128-129 5.5875 0.0 0.0 0.0 0.025 130-131 6.15 0.0 0.0 0.0 0.025 132-133 6.85 0.0 0.0 0.0 0.025 134-135 7.575 0.0 0.0 0.0 0.025 136-137 8.412500000000001 0.0 0.0 0.0 0.025 138-139 9.375 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTTTC 10 0.006875036 144.6875 1 TCGCATA 10 0.006875036 144.6875 145 TTTGGAG 10 0.006875036 144.6875 2 TTGGAGT 10 0.006875036 144.6875 3 GCTTTCT 15 1.1509859E-4 144.6875 2 CTTTCTT 50 1.4006218E-10 101.28124 1 TTTCTTT 55 2.9649527E-10 92.07386 2 GATATCA 55 2.9649527E-10 92.07386 145 TTCTTTT 55 2.9649527E-10 92.07386 3 TCTTTTC 55 2.9649527E-10 92.07386 4 CTTTTCT 60 5.9299055E-10 84.40104 5 TCTTCAA 65 1.1186785E-9 77.90865 9 TTTCTTC 70 2.0136213E-9 72.34375 7 TTTTCTT 75 3.4760888E-9 67.520836 6 TTCTTCA 75 3.4760888E-9 67.520836 8 TGATATC 50 2.139257E-6 23.15 140-144 CTTCAAA 65 9.0677895E-7 22.259615 6 TTCAAAA 65 9.0677895E-7 22.259615 7 TCAAAAA 65 9.0677895E-7 22.259615 8 CAAAAAT 70 7.464601E-4 20.669643 9 >>END_MODULE SRR8846509 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846509_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.58925 33.0 33.0 34.0 32.0 34.0 2 32.88275 33.0 33.0 34.0 32.0 34.0 3 32.885 33.0 33.0 34.0 32.0 34.0 4 32.78725 33.0 33.0 34.0 32.0 34.0 5 32.807 33.0 33.0 34.0 32.0 34.0 6 36.92275 38.0 38.0 38.0 36.0 38.0 7 36.9925 38.0 38.0 38.0 36.0 38.0 8 36.99725 38.0 38.0 38.0 36.0 38.0 9 37.09 38.0 38.0 38.0 36.0 38.0 10-14 37.01625 38.0 38.0 38.0 36.0 38.0 15-19 36.955799999999996 38.0 38.0 38.0 36.0 38.0 20-24 36.9612 38.0 38.0 38.0 36.0 38.0 25-29 36.88005 38.0 38.0 38.0 36.0 38.0 30-34 36.88465 38.0 38.0 38.0 36.0 38.0 35-39 36.9177 38.0 38.0 38.0 36.0 38.0 40-44 36.87385 38.0 38.0 38.0 36.0 38.0 45-49 36.854000000000006 38.0 38.0 38.0 35.6 38.0 50-54 36.693 38.0 38.0 38.0 35.2 38.0 55-59 36.67915 38.0 38.0 38.0 35.0 38.0 60-64 36.58235 38.0 38.0 38.0 34.4 38.0 65-69 36.52685 38.0 38.0 38.0 34.4 38.0 70-74 36.59435 38.0 38.0 38.0 34.8 38.0 75-79 36.50295 38.0 38.0 38.0 34.2 38.0 80-84 36.3899 38.0 38.0 38.0 34.0 38.0 85-89 36.33435 38.0 38.0 38.0 34.0 38.0 90-94 36.216249999999995 38.0 37.6 38.0 33.6 38.0 95-99 36.0503 38.0 37.8 38.0 33.0 38.0 100-104 35.9423 38.0 37.0 38.0 33.0 38.0 105-109 35.768449999999994 38.0 37.0 38.0 31.4 38.0 110-114 35.493050000000004 38.0 36.8 38.0 30.2 38.0 115-119 35.59715 38.0 36.8 38.0 31.0 38.0 120-124 35.335800000000006 38.0 36.0 38.0 29.6 38.0 125-129 35.050850000000004 38.0 35.8 38.0 28.0 38.0 130-134 34.71645 38.0 35.2 38.0 27.4 38.0 135-139 34.20955 38.0 35.0 38.0 23.6 38.0 140-144 33.758 38.0 34.8 38.0 21.8 38.0 145-149 32.590650000000004 38.0 33.6 38.0 11.4 38.0 150-151 28.293875 35.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 1.0 4 0.0 5 0.0 6 1.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 3.0 14 1.0 15 2.0 16 2.0 17 3.0 18 7.0 19 2.0 20 9.0 21 5.0 22 3.0 23 10.0 24 20.0 25 24.0 26 30.0 27 38.0 28 39.0 29 46.0 30 45.0 31 65.0 32 92.0 33 129.0 34 196.0 35 308.0 36 596.0 37 2309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.852130325814535 18.646616541353385 17.36842105263158 43.1328320802005 2 20.485850237916353 22.01352366641623 43.15051339844728 14.350112697220135 3 18.50738792887553 25.720010017530683 36.764337590783875 19.00826446280992 4 22.533800701051575 28.84326489734602 28.743114672008012 19.87981972959439 5 23.240671174555473 32.83245679939895 30.47833708990734 13.448534936138243 6 19.739478957915832 34.71943887775551 29.80961923847695 15.731462925851702 7 16.791979949874687 19.147869674185465 47.418546365914786 16.641604010025063 8 19.789579158316634 23.446893787575153 36.84869739478958 19.914829659318638 9 20.852130325814535 18.922305764411025 38.796992481203006 21.428571428571427 10-14 22.23670359416512 26.98882149481177 31.64569652614166 19.128778384881446 15-19 22.855137140851426 26.21471192899764 31.504788647645793 19.42536228250514 20-24 22.458901363271853 26.518644747393743 31.921611868484362 19.10084202085004 25-29 22.819330258672547 25.70683777822338 31.872869460597553 19.600962502506516 30-34 23.794486215538846 25.48370927318296 31.71428571428571 19.007518796992482 35-39 23.750563938042006 26.141661236152185 30.40252644242819 19.70524838337761 40-44 21.89989478430783 26.809960418858658 31.504584398015933 19.785560398817577 45-49 22.10368144252442 27.803656398697722 30.88905584773353 19.20360631104433 50-54 21.826470293557758 27.13655946297966 30.863640917743712 20.173329325718868 55-59 21.2625250501002 26.833667334669336 30.66633266533066 21.2374749498998 60-64 21.23716503881793 25.91034310042574 31.995992987728528 20.8564988730278 65-69 21.863260706235913 26.831955922865014 30.758827948910593 20.54595542198848 70-74 22.163786626596544 26.51139494114701 31.43000250438267 19.89481592787378 75-79 22.030421294906436 26.273391373961775 31.401981386970878 20.29420594416091 80-84 22.621014166291236 25.739600540621716 32.482354707914105 19.157030585172947 85-89 21.833208114199852 27.59328825444528 30.242925118958176 20.330578512396695 90-94 22.36248872858431 26.55044584710951 30.608155495441338 20.478909928864844 95-99 22.282908252743397 26.857744149922336 30.721050258054817 20.13829733927945 100-104 23.19102024453798 26.813990779715375 30.682501503307275 19.312487472439365 105-109 23.5644854193807 26.14991482112436 30.509069044994487 19.776530714500453 110-114 23.12509393316968 26.737137417965034 29.953409147838283 20.184359501027004 115-119 22.503882571013474 26.91247933470267 30.61970843144131 19.963929662842542 120-124 22.529426496368647 27.95892812421738 29.020786376158274 20.4908590032557 125-129 23.363549857264488 27.47032603796264 29.57880502829669 19.587319076476188 130-134 22.834961182068618 28.239418983220638 29.60681192086151 19.318807913849238 135-139 24.35884592266079 27.3893007413344 29.24263674614306 19.00921658986175 140-144 24.6318741861164 28.313132324952416 28.688770910547934 18.36622257838325 145-149 24.188539370867563 27.31917451412543 28.95211380484873 19.540172310158287 150-151 24.896784686600775 27.53659452020518 29.475791317402727 18.090829475791317 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.5 3 0.5 4 1.0 5 1.0 6 1.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.5 20 2.5 21 5.0 22 7.5 23 8.0 24 9.0 25 18.0 26 25.5 27 31.0 28 36.0 29 49.0 30 60.0 31 66.0 32 68.0 33 76.0 34 106.5 35 145.5 36 151.0 37 173.5 38 214.5 39 227.5 40 249.5 41 262.0 42 238.0 43 225.0 44 222.0 45 198.5 46 171.0 47 135.5 48 113.5 49 88.5 50 71.0 51 55.0 52 34.5 53 25.0 54 19.5 55 24.5 56 31.0 57 23.5 58 16.5 59 34.5 60 45.0 61 32.0 62 22.0 63 32.0 64 34.5 65 30.5 66 24.5 67 11.5 68 8.5 69 4.0 70 2.0 71 5.5 72 5.0 73 3.5 74 4.0 75 2.0 76 1.5 77 1.5 78 0.5 79 0.5 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.17500000000000002 3 0.17500000000000002 4 0.15 5 0.17500000000000002 6 0.2 7 0.25 8 0.2 9 0.25 10-14 0.255 15-19 0.28500000000000003 20-24 0.24 25-29 0.26 30-34 0.25 35-39 0.255 40-44 0.20500000000000002 45-49 0.17500000000000002 50-54 0.19 55-59 0.2 60-64 0.17500000000000002 65-69 0.17500000000000002 70-74 0.17500000000000002 75-79 0.06999999999999999 80-84 0.11499999999999999 85-89 0.17500000000000002 90-94 0.19 95-99 0.215 100-104 0.22 105-109 0.21 110-114 0.19499999999999998 115-119 0.19499999999999998 120-124 0.17500000000000002 125-129 0.165 130-134 0.17500000000000002 135-139 0.18 140-144 0.16999999999999998 145-149 0.18 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.575 #Duplication Level Percentage of deduplicated Percentage of total 1 85.44102019128587 60.3 2 7.686857952532766 10.85 3 2.302515054906128 4.875 4 1.487778958554729 4.2 5 0.5313496280552604 1.875 6 0.5313496280552604 2.25 7 0.495926319518243 2.45 8 0.2479631597591215 1.4000000000000001 9 0.10626992561105207 0.675 >10 1.1689691817215728 11.125 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 43 1.075 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 23 0.575 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 19 0.475 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 17 0.42500000000000004 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 17 0.42500000000000004 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 15 0.375 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 14 0.35000000000000003 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 13 0.325 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 13 0.325 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 13 0.325 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 13 0.325 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 13 0.325 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 13 0.325 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 12 0.3 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 12 0.3 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 12 0.3 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 11 0.27499999999999997 No Hit CGTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGAC 11 0.27499999999999997 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 11 0.27499999999999997 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 11 0.27499999999999997 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 11 0.27499999999999997 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 11 0.27499999999999997 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 11 0.27499999999999997 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 11 0.27499999999999997 No Hit CCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATA 11 0.27499999999999997 No Hit CAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTT 10 0.25 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 10 0.25 No Hit CTGTTCTATAGGATCGTACCGCTACATCCTTTACCAAAAAGGAGGCAAGA 10 0.25 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 10 0.25 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 10 0.25 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 10 0.25 No Hit TTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATAC 9 0.22499999999999998 No Hit CTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCAC 9 0.22499999999999998 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 9 0.22499999999999998 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 8 0.2 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 8 0.2 No Hit CTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTA 8 0.2 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 8 0.2 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 8 0.2 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 8 0.2 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 7 0.17500000000000002 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 7 0.17500000000000002 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 7 0.17500000000000002 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 7 0.17500000000000002 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 7 0.17500000000000002 No Hit CTTTGGTACAAAATTGACAATCTCACAAGGATGAAATACCAGTAATTTTT 7 0.17500000000000002 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 7 0.17500000000000002 No Hit CTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGT 7 0.17500000000000002 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 7 0.17500000000000002 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 7 0.17500000000000002 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 7 0.17500000000000002 No Hit GTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACTGAATTTAAAATG 7 0.17500000000000002 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 6 0.15 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 6 0.15 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 6 0.15 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit CGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCC 6 0.15 No Hit CTTGGTATGGAAGTAATGCACGAACGTAATGCTCACAACTTCCCTCTAGA 6 0.15 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 6 0.15 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit GATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAA 6 0.15 No Hit GTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCT 6 0.15 No Hit CTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTA 6 0.15 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 6 0.15 No Hit CGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATG 6 0.15 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6 0.15 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit AGCTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGG 5 0.125 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 5 0.125 No Hit TAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAA 5 0.125 No Hit TATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAG 5 0.125 No Hit CTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCT 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit CAACGATTAAATTGAATTTATGGCTACGCAAACCGTTGAAGATAGTTCTA 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 5 0.125 No Hit CTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAAC 5 0.125 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.175 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.21250000000000002 0.0 0.0 0.0 0.0 94-95 0.275 0.0 0.0 0.0 0.0 96-97 0.35 0.0 0.0 0.0 0.0 98-99 0.475 0.0 0.0 0.0 0.0 100-101 0.675 0.0 0.0 0.0 0.0 102-103 0.8875 0.0 0.0 0.0 0.0 104-105 1.0625 0.0 0.0 0.0 0.0 106-107 1.2625000000000002 0.0 0.0 0.0 0.0 108-109 1.5125 0.0 0.0 0.0 0.0 110-111 1.8 0.0 0.0 0.0 0.0 112-113 2.025 0.0 0.0 0.0 0.0 114-115 2.2625 0.0 0.0 0.0 0.0 116-117 2.625 0.0 0.0 0.0 0.0 118-119 3.0 0.0 0.0 0.0 0.0 120-121 3.45 0.0 0.0 0.0 0.0 122-123 3.8375000000000004 0.0 0.0 0.0 0.0 124-125 4.4 0.0 0.0 0.0 0.0 126-127 5.025 0.0 0.0 0.0 0.0 128-129 5.625 0.0 0.0 0.0 0.0 130-131 6.225 0.0 0.0 0.0 0.0 132-133 6.9125 0.0 0.0 0.0 0.0 134-135 7.612500000000001 0.0 0.0 0.0 0.0 136-137 8.425 0.0 0.0 0.0 0.0 138-139 9.350000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAAGGA 10 0.006843168 144.91249 1 >>END_MODULE Read 1653686 spots for SRR8846509.sra Written 1653686 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra Read 1653668 spots for SRR8846509.sra Written 1653668 spots for SRR8846509.sra SRR ids: ['SRR8846509.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_t47ixj8i SRR8846509.sra spots: 33073378 blocks: [[1, 1653668], [1653669, 3307336], [3307337, 4961004], [4961005, 6614672], [6614673, 8268340], [8268341, 9922008], [9922009, 11575676], [11575677, 13229344], [13229345, 14883012], [14883013, 16536680], [16536681, 18190348], [18190349, 19844016], [19844017, 21497684], [21497685, 23151352], [23151353, 24805020], [24805021, 26458688], [26458689, 28112356], [28112357, 29766024], [29766025, 31419692], [31419693, 33073378]] SRR8846509 file size 11185782 SRR8846509 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846509 SRR8846509_1.fastq SRR8846509_2.fastq Input file: SRR8846509_1.fastq Paired file: SRR8846509_2.fastq trimmed: SRR8846509-trimmed-pair1.fastq, SRR8846509-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 04:07:25 2024 >> started Mon Dec 9 04:11:21 2024 >> done (236.107s) 33073378 read pairs processed; of these: 11901 ( 0.04%) short read pairs filtered out after trimming by size control 98409 ( 0.30%) empty read pairs filtered out after trimming by size control 32963068 (99.67%) read pairs available; of these: 14355092 (43.55%) trimmed read pairs available after processing 18607976 (56.45%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 4 0.00% 20 4 0.00% 21 5 0.00% 22 12 0.00% 23 7 0.00% 24 4 0.00% 25 7 0.00% 26 11 0.00% 27 38 0.00% 28 11 0.00% 29 9 0.00% 30 10 0.00% 31 12 0.00% 32 13 0.00% 33 8 0.00% 34 15 0.00% 35 13 0.00% 36 11 0.00% 37 10 0.00% 38 25 0.00% 39 21 0.00% 40 19 0.00% 41 24 0.00% 42 36 0.00% 43 34 0.00% 44 40 0.00% 45 34 0.00% 46 44 0.00% 47 38 0.00% 48 74 0.00% 49 64 0.00% 50 76 0.00% 51 85 0.00% 52 121 0.00% 53 91 0.00% 54 142 0.00% 55 136 0.00% 56 150 0.00% 57 189 0.00% 58 175 0.00% 59 249 0.00% 60 272 0.00% 61 295 0.00% 62 357 0.00% 63 363 0.00% 64 484 0.00% 65 498 0.00% 66 567 0.00% 67 566 0.00% 68 704 0.00% 69 784 0.00% 70 855 0.00% 71 982 0.00% 72 1108 0.00% 73 1229 0.00% 74 1454 0.00% 75 1748 0.01% 76 1789 0.01% 77 2069 0.01% 78 2218 0.01% 79 2393 0.01% 80 2775 0.01% 81 3361 0.01% 82 3915 0.01% 83 4309 0.01% 84 5020 0.02% 85 6471 0.02% 86 6785 0.02% 87 7389 0.02% 88 7837 0.02% 89 8471 0.03% 90 10421 0.03% 91 10613 0.03% 92 12621 0.04% 93 14165 0.04% 94 15972 0.05% 95 17188 0.05% 96 18188 0.06% 97 19374 0.06% 98 22516 0.07% 99 24650 0.07% 100 26310 0.08% 101 29061 0.09% 102 32272 0.10% 103 33438 0.10% 104 38184 0.12% 105 44488 0.13% 106 47811 0.15% 107 50723 0.15% 108 51319 0.16% 109 60125 0.18% 110 59630 0.18% 111 66481 0.20% 112 66737 0.20% 113 64687 0.20% 114 69102 0.21% 115 73553 0.22% 116 82857 0.25% 117 88775 0.27% 118 89328 0.27% 119 98318 0.30% 120 99243 0.30% 121 102795 0.31% 122 106225 0.32% 123 106675 0.32% 124 116156 0.35% 125 132848 0.40% 126 125474 0.38% 127 136793 0.41% 128 147475 0.45% 129 159957 0.49% 130 153349 0.47% 131 173194 0.53% 132 158355 0.48% 133 163274 0.50% 134 170486 0.52% 135 169543 0.51% 136 185645 0.56% 137 187184 0.57% 138 208706 0.63% 139 213192 0.65% 140 218510 0.66% 141 254454 0.77% 142 233520 0.71% 143 261091 0.79% 144 285454 0.87% 145 342852 1.04% 146 351874 1.07% 147 435026 1.32% 148 596484 1.81% 149 1071761 3.25% 150 5869437 17.81% 151 18607976 56.45% 32963068 reads passed initial QC criterion=sequence-density sequence-density=3.75 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=37 prefix-density=3.64 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=1633.86 fanout-score-rank=1 prefix-density=12.06 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=2.11 sequence-density-rank=1 fanout-score=1.81 fanout-score-rank=31 prefix-density=3.77 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=90.90 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=6.6 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x CCAGCCTCACGC -y TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG -o SRR8846509 SRR8846509_1.fastq SRR8846509_2.fastq Input file: SRR8846509_1.fastq Paired file: SRR8846509_2.fastq trimmed: SRR8846509-trimmed-pair1.fastq, SRR8846509-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): CCAGCCTCACGC -- paired 3' end adapter sequence (-y): TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 04:27:56 2024 >> started Mon Dec 9 04:29:58 2024 >> done (122.650s) 16481534 read pairs processed; of these: 2846 ( 0.02%) short read pairs filtered out after trimming by size control 7865 ( 0.05%) empty read pairs filtered out after trimming by size control 16470823 (99.94%) read pairs available; of these: 2669 ( 0.02%) trimmed read pairs available after processing 16468154 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 8 0.00% 19 1 0.00% 20 2 0.00% 21 0 0.00% 22 6 0.00% 23 2 0.00% 24 2 0.00% 25 5 0.00% 26 9 0.00% 27 17 0.00% 28 5 0.00% 29 5 0.00% 30 5 0.00% 31 8 0.00% 32 10 0.00% 33 4 0.00% 34 9 0.00% 35 7 0.00% 36 6 0.00% 37 9 0.00% 38 13 0.00% 39 12 0.00% 40 10 0.00% 41 11 0.00% 42 20 0.00% 43 16 0.00% 44 20 0.00% 45 23 0.00% 46 20 0.00% 47 18 0.00% 48 40 0.00% 49 31 0.00% 50 30 0.00% 51 37 0.00% 52 65 0.00% 53 54 0.00% 54 77 0.00% 55 65 0.00% 56 75 0.00% 57 110 0.00% 58 95 0.00% 59 133 0.00% 60 144 0.00% 61 137 0.00% 62 190 0.00% 63 185 0.00% 64 235 0.00% 65 253 0.00% 66 281 0.00% 67 296 0.00% 68 344 0.00% 69 390 0.00% 70 442 0.00% 71 499 0.00% 72 541 0.00% 73 628 0.00% 74 740 0.00% 75 846 0.01% 76 901 0.01% 77 1061 0.01% 78 1149 0.01% 79 1180 0.01% 80 1388 0.01% 81 1670 0.01% 82 1986 0.01% 83 2139 0.01% 84 2498 0.02% 85 3224 0.02% 86 3321 0.02% 87 3719 0.02% 88 3951 0.02% 89 4182 0.03% 90 5168 0.03% 91 5212 0.03% 92 6312 0.04% 93 6964 0.04% 94 8144 0.05% 95 8555 0.05% 96 9159 0.06% 97 9691 0.06% 98 11166 0.07% 99 12362 0.08% 100 13131 0.08% 101 14566 0.09% 102 16091 0.10% 103 16818 0.10% 104 18994 0.12% 105 22209 0.13% 106 23769 0.14% 107 25237 0.15% 108 25382 0.15% 109 30081 0.18% 110 30024 0.18% 111 33042 0.20% 112 33376 0.20% 113 32198 0.20% 114 34457 0.21% 115 37085 0.23% 116 41773 0.25% 117 44241 0.27% 118 44698 0.27% 119 49454 0.30% 120 49576 0.30% 121 50863 0.31% 122 53102 0.32% 123 53371 0.32% 124 58376 0.35% 125 66494 0.40% 126 62763 0.38% 127 68161 0.41% 128 74027 0.45% 129 80014 0.49% 130 76464 0.46% 131 86367 0.52% 132 79166 0.48% 133 81666 0.50% 134 85165 0.52% 135 84560 0.51% 136 93142 0.57% 137 93546 0.57% 138 104061 0.63% 139 106439 0.65% 140 109408 0.66% 141 127614 0.77% 142 116700 0.71% 143 130584 0.79% 144 142367 0.86% 145 171045 1.04% 146 176202 1.07% 147 216951 1.32% 148 298047 1.81% 149 535386 3.25% 150 2931785 17.80% 151 9298437 56.45% criterion=sequence-density sequence-density=3.67 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=37 prefix-density=3.62 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=1749.60 fanout-score-rank=1 prefix-density=12.23 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=2.07 sequence-density-rank=1 fanout-score=1.80 fanout-score-rank=33 prefix-density=3.70 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=94.01 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=6.6 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR8846509 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 04:36:34 Started mapping on | Dec 09 04:36:35 Finished on | Dec 09 05:02:42 Mapping speed, Million of reads per hour | 75.70 Number of input reads | 32952357 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 20583057 Uniquely mapped reads % | 62.46% Average mapped length | 293.76 Number of splices: Total | 4145296 Number of splices: Annotated (sjdb) | 3716833 Number of splices: GT/AG | 3973488 Number of splices: GC/AG | 45772 Number of splices: AT/AC | 15974 Number of splices: Non-canonical | 110062 Mismatch rate per base, % | 0.19% Deletion rate per base | 0.01% Deletion average length | 1.65 Insertion rate per base | 0.02% Insertion average length | 1.74 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11137319 % of reads mapped to multiple loci | 33.80% Number of reads mapped to too many loci | 4411 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.20% % of reads unmapped: other | 0.52% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1237752 1237752 1237752 N_multimapping 11137319 11137319 11137319 N_noFeature 5373991 19710373 5771786 N_ambiguous 978962 39136 501638 UnstrandedReadsAssigned:14230104 PositiveStrandReadsAssigned:833548 NegativeStrandReadsAssigned:14309633 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR8846509 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846509-trimmed-pair1.fastq SRR8846509-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,952,357 reads, 19,281,214 reads pseudoaligned [quant] estimated average fragment length: 210.691 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,081 rounds 52973 SRR8846509.ke.tsv 35125 SRR8846509.se.tsv 88098 total ==> SRR8846509.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 726.896 0 0 PNS24247 1044 834.309 7.86571 0.507027 PNS24249 1928 1718.31 20.2219 0.632907 PNS24246 1044 834.309 7.86571 0.507027 PNS24248 1044 834.309 7.86571 0.507027 PNS24244 1471 1261.31 49.181 2.09699 PNS24243 293 109.11 0 0 KQK14069 1603 1393.31 1437.23 55.4752 KQK14071 474 270.752 1.61608 0.321004 ==> SRR8846509.se.tsv <== BRADI_1g14170v3 1594 BRADI_1g53295v3 23 BRADI_1g59795v3 39 BRADI_1g07683v3 0 BRADI_1g00485v3 8 BRADI_1g20270v3 309 BRADI_1g74790v3 41 BRADI_1g09890v3 1 BRADI_1g77505v3 64 BRADI_1g48960v3 0 SRR8846509 completed mapping pipeline successfully