Starting /dee2/code/volunteer_pipeline.sh SRR8846510 current disk space = 1506683899904 free memory = 1406809612 SRR8846510 SRAfilesize 493964c29b11a2bf18398d366e608224 SRR8846510.sra SRR8846510.sra file validated SRR8846510 is paired end SRR8846510 is conventional basespace SRR8846510 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846510_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.28125 34.0 33.0 34.0 33.0 34.0 2 33.286 34.0 33.0 34.0 33.0 34.0 3 33.27075 34.0 33.0 34.0 33.0 34.0 4 33.2535 34.0 33.0 34.0 32.0 34.0 5 33.18425 34.0 33.0 34.0 32.0 34.0 6 36.72225 38.0 37.0 38.0 34.0 38.0 7 37.10525 38.0 38.0 38.0 36.0 38.0 8 37.1995 38.0 38.0 38.0 36.0 38.0 9 37.273 38.0 38.0 38.0 37.0 38.0 10-14 37.25615 38.0 38.0 38.0 36.6 38.0 15-19 37.28215 38.0 38.0 38.0 36.8 38.0 20-24 37.319900000000004 38.0 38.0 38.0 37.0 38.0 25-29 37.238499999999995 38.0 38.0 38.0 36.8 38.0 30-34 37.2153 38.0 38.0 38.0 36.2 38.0 35-39 37.17470000000001 38.0 38.0 38.0 36.2 38.0 40-44 37.1803 38.0 38.0 38.0 36.0 38.0 45-49 37.15045 38.0 38.0 38.0 36.0 38.0 50-54 36.99225 38.0 38.0 38.0 35.8 38.0 55-59 36.5916 38.0 38.0 38.0 35.2 38.0 60-64 35.6195 38.0 38.0 38.0 33.4 38.0 65-69 36.412600000000005 38.0 38.0 38.0 33.8 38.0 70-74 36.85045 38.0 38.0 38.0 35.0 38.0 75-79 36.78125 38.0 38.0 38.0 35.0 38.0 80-84 36.5791 38.0 38.0 38.0 34.0 38.0 85-89 36.48825 38.0 38.0 38.0 34.0 38.0 90-94 36.431149999999995 38.0 38.0 38.0 34.0 38.0 95-99 36.38835 38.0 38.0 38.0 34.0 38.0 100-104 36.0409 38.0 37.2 38.0 32.6 38.0 105-109 36.0443 38.0 37.0 38.0 33.0 38.0 110-114 35.90005 38.0 37.0 38.0 31.8 38.0 115-119 35.68645 38.0 37.0 38.0 31.0 38.0 120-124 35.4186 38.0 36.2 38.0 29.8 38.0 125-129 35.0054 38.0 35.4 38.0 28.4 38.0 130-134 34.86015 38.0 35.6 38.0 27.8 38.0 135-139 34.2076 38.0 34.6 38.0 24.0 38.0 140-144 33.62025 38.0 33.0 38.0 22.2 38.0 145-149 33.00265 38.0 33.0 38.0 16.2 38.0 150-151 28.852625 35.5 24.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 2.0 16 1.0 17 1.0 18 3.0 19 2.0 20 2.0 21 4.0 22 7.0 23 10.0 24 22.0 25 20.0 26 21.0 27 34.0 28 47.0 29 52.0 30 59.0 31 77.0 32 98.0 33 109.0 34 209.0 35 331.0 36 612.0 37 2276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.6 9.575 8.525 57.3 2 17.775 11.95 46.825 23.45 3 14.85 17.349999999999998 31.3 36.5 4 21.6 24.625 28.000000000000004 25.775 5 21.391043282461847 30.522892169126848 29.372029021766327 18.714035526644984 6 17.375 34.775 29.675 18.175 7 11.575000000000001 27.750000000000004 46.35 14.325 8 15.625 27.075 38.675 18.625 9 15.950000000000001 20.775 41.5 21.775 10-14 17.825 33.78 26.415 21.98 15-19 18.652797919687952 31.499724958743812 28.764314647197082 21.083162474371157 20-24 18.05 31.119999999999997 29.154999999999998 21.675 25-29 20.905 30.599999999999998 28.29 20.205000000000002 30-34 22.900000000000002 31.2 25.22 20.68 35-39 20.19 32.934999999999995 26.075 20.8 40-44 18.509999999999998 30.945 28.12 22.425 45-49 18.335 31.385 28.000000000000004 22.28 50-54 19.27 31.845000000000002 28.249999999999996 20.635 55-59 21.554734551343692 30.224201629637125 25.48711979351182 22.733944025507363 60-64 18.11575395795484 32.509732675837014 27.98858032701791 21.385933039190242 65-69 21.54248629068773 32.06721336217739 24.8930925189918 21.49720782814308 70-74 21.68 31.035 24.92 22.365 75-79 20.560000000000002 31.69 26.825 20.925 80-84 23.255 30.630000000000003 25.074999999999996 21.04 85-89 22.12 30.5 26.5 20.880000000000003 90-94 21.477147714771476 30.468046804680466 27.222722272227223 20.83208320832083 95-99 20.64 31.424999999999997 26.525 21.41 100-104 19.384999999999998 31.04 26.889999999999997 22.685 105-109 21.29 28.105000000000004 27.575 23.03 110-114 19.56 29.609999999999996 28.225 22.605 115-119 18.63593179658983 30.4115205760288 27.711385569278463 23.241162058102905 120-124 17.26 32.445 25.290000000000003 25.005 125-129 19.997999699954995 32.234835225283796 24.898734810221534 22.86843026453968 130-134 21.628244236635496 31.814772215832377 25.13877081562234 21.418212731909787 135-139 22.73068267066767 30.19754938734684 25.07126781695424 22.00050012503126 140-144 21.807180718071805 30.698069806980698 27.082708270827084 20.412041204120413 145-149 20.97 31.014999999999997 26.150000000000002 21.865000000000002 150-151 21.325 30.9375 25.025 22.7125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 1.0 20 1.5 21 2.0 22 6.5 23 9.0 24 10.0 25 9.5 26 16.5 27 27.0 28 37.0 29 42.0 30 48.0 31 57.5 32 67.0 33 72.0 34 86.5 35 109.0 36 153.5 37 318.0 38 337.5 39 203.5 40 215.0 41 231.0 42 210.5 43 258.0 44 240.5 45 194.5 46 158.5 47 110.5 48 110.5 49 86.0 50 59.0 51 48.5 52 36.5 53 30.0 54 30.0 55 33.5 56 31.5 57 21.5 58 25.0 59 30.5 60 35.0 61 35.0 62 21.5 63 15.0 64 25.5 65 36.5 66 21.5 67 5.0 68 5.0 69 5.0 70 5.0 71 4.0 72 1.0 73 2.5 74 4.0 75 2.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.075 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.015 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.205 60-64 3.675 65-69 0.615 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.005 120-124 0.0 125-129 0.015 130-134 0.015 135-139 0.025 140-144 0.01 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.0 #Duplication Level Percentage of deduplicated Percentage of total 1 86.55797101449275 59.724999999999994 2 7.355072463768115 10.15 3 2.5 5.175 4 0.8695652173913043 2.4 5 0.7608695652173914 2.625 6 0.2898550724637681 1.2 7 0.5072463768115941 2.45 8 0.14492753623188406 0.8 9 0.2898550724637681 1.7999999999999998 >10 0.6521739130434783 8.175 >50 0.0 0.0 >100 0.07246376811594203 5.5 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 116 2.9000000000000004 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 104 2.6 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 48 1.2 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 38 0.95 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 29 0.7250000000000001 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 24 0.6 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 20 0.5 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 17 0.42500000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 15 0.375 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 15 0.375 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 15 0.375 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 14 0.35000000000000003 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 13 0.325 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 12 0.3 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 12 0.3 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 12 0.3 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 11 0.27499999999999997 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 11 0.27499999999999997 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 11 0.27499999999999997 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 10 0.25 No Hit CTTTTTTTGATTGTCTGTCAATCAATATTCTAATGGCAATGCAATTTCAT 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 9 0.22499999999999998 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 9 0.22499999999999998 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 9 0.22499999999999998 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 9 0.22499999999999998 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 9 0.22499999999999998 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 9 0.22499999999999998 No Hit GTGGTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCG 9 0.22499999999999998 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 8 0.2 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 8 0.2 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 7 0.17500000000000002 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 7 0.17500000000000002 No Hit CTCGGTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTT 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit CTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAA 7 0.17500000000000002 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 7 0.17500000000000002 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 7 0.17500000000000002 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 7 0.17500000000000002 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 7 0.17500000000000002 No Hit GTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCC 7 0.17500000000000002 No Hit CTTTGGAGTAGGCTATGAGACCCAAGCGGGCCAGGAATGCAGCGGCCCGT 7 0.17500000000000002 No Hit CCCTTATCCTCATTACGCCTAGCATTTAGTGGGCTGGATATTTACCTTAT 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 6 0.15 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 6 0.15 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit CTCTGTAACAGTTGGGCGATAGTAAAAAGTCATAGCAAAACCGGTAGCGA 5 0.125 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 5 0.125 No Hit CTAAAATATCGGTATTAAGCCCGAAACTGCCAGCGGATGGCCAGTGAGCT 5 0.125 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 5 0.125 No Hit GTCCAAACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGC 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit TTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAG 5 0.125 No Hit CCAAACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCC 5 0.125 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 5 0.125 No Hit CTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGAT 5 0.125 No Hit GTCGTGAATAGCTCCGTGGAATAAAATAGAATTTCCTTATGCATAGAACT 5 0.125 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 5 0.125 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.0875 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.1125 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.15 0.0 0.0 0.0 0.0 94-95 0.225 0.0 0.0 0.0 0.0 96-97 0.3125 0.0 0.0 0.0 0.0 98-99 0.5125 0.0 0.0 0.0 0.0 100-101 0.725 0.0 0.0 0.0 0.0 102-103 0.9125 0.0 0.0 0.0 0.0 104-105 1.0375 0.0 0.0 0.0 0.0 106-107 1.4375 0.0 0.0 0.0 0.0 108-109 1.925 0.0 0.0 0.0 0.0 110-111 2.4000000000000004 0.0 0.0 0.0 0.0 112-113 2.675 0.0 0.0 0.0 0.0 114-115 3.1500000000000004 0.0 0.0 0.0 0.0 116-117 3.5125 0.0 0.0 0.0 0.0 118-119 3.9375 0.0 0.0 0.0 0.0 120-121 4.487500000000001 0.0 0.0 0.0 0.0 122-123 5.1625 0.0 0.0 0.0 0.0 124-125 5.6375 0.0 0.0 0.0 0.0 126-127 6.3125 0.0 0.0 0.0 0.0 128-129 7.025 0.0 0.0 0.0 0.0 130-131 7.925 0.0 0.0 0.0 0.0 132-133 8.6375 0.0 0.0 0.0 0.0 134-135 9.25 0.0 0.0 0.0 0.0 136-137 9.8625 0.0 0.0 0.0 0.0 138-139 10.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAGCCC 40 5.1706957E-7 90.328125 145 TCTTTTC 35 0.0033557771 61.93929 4 CTTTCTT 35 0.0033557771 61.93929 1 TTTCTTC 65 9.263224E-6 55.58654 3 TTCTTCA 65 9.263224E-6 55.58654 4 CTTTTCT 70 1.4371064E-5 51.616074 1 CTTCAAA 70 1.4371064E-5 51.616074 6 TTTTCTT 70 1.4371064E-5 51.616074 2 TTTCTTT 45 0.009074212 48.175 2 TTCAAAA 75 2.1621572E-5 48.175 7 TCAAAAA 75 2.1621572E-5 48.175 8 TTCTTTT 45 0.009074212 48.175 3 TCTTCAA 75 2.1621572E-5 48.175 5 CAAAAAT 85 4.531806E-5 42.50735 9 TGATATC 60 2.3291756E-4 24.0875 145 GATATCA 50 5.7522753E-5 20.2335 140-144 TTAGCGG 75 1.6543345E-7 19.27 25-29 ATCAGCC 45 6.6974835E-4 19.269999 140-144 TATCAGC 45 6.6974835E-4 19.269999 140-144 ATATCAG 45 6.6974835E-4 19.269999 140-144 >>END_MODULE SRR8846510 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846510_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30075 33.0 33.0 34.0 31.0 34.0 2 32.552 33.0 33.0 34.0 31.0 34.0 3 32.6085 33.0 33.0 34.0 32.0 34.0 4 32.4615 33.0 33.0 34.0 31.0 34.0 5 32.42525 33.0 33.0 34.0 31.0 34.0 6 36.55175 38.0 38.0 38.0 34.0 38.0 7 36.5895 38.0 38.0 38.0 35.0 38.0 8 36.6775 38.0 38.0 38.0 35.0 38.0 9 36.59675 38.0 38.0 38.0 35.0 38.0 10-14 36.52605 38.0 38.0 38.0 34.4 38.0 15-19 36.441250000000004 38.0 38.0 38.0 34.0 38.0 20-24 36.445550000000004 38.0 38.0 38.0 34.0 38.0 25-29 36.31305 38.0 38.0 38.0 34.0 38.0 30-34 36.31815 38.0 38.0 38.0 34.0 38.0 35-39 36.34435 38.0 38.0 38.0 34.0 38.0 40-44 36.331599999999995 38.0 38.0 38.0 34.0 38.0 45-49 36.248149999999995 38.0 38.0 38.0 33.6 38.0 50-54 36.04535 38.0 37.8 38.0 32.8 38.0 55-59 36.070800000000006 38.0 38.0 38.0 33.0 38.0 60-64 35.918549999999996 38.0 37.0 38.0 31.6 38.0 65-69 35.9229 38.0 37.0 38.0 31.4 38.0 70-74 35.95085 38.0 37.0 38.0 32.6 38.0 75-79 35.757799999999996 38.0 37.0 38.0 31.2 38.0 80-84 35.675 38.0 37.0 38.0 30.4 38.0 85-89 35.6181 38.0 37.0 38.0 30.4 38.0 90-94 35.4424 38.0 36.6 38.0 29.2 38.0 95-99 35.3044 38.0 36.2 38.0 29.0 38.0 100-104 35.0779 38.0 36.0 38.0 28.0 38.0 105-109 34.82335 38.0 36.0 38.0 27.0 38.0 110-114 34.5865 38.0 35.2 38.0 25.4 38.0 115-119 34.3709 38.0 35.0 38.0 24.2 38.0 120-124 34.11695 38.0 34.8 38.0 23.0 38.0 125-129 33.85425 38.0 34.6 38.0 22.2 38.0 130-134 33.493399999999994 38.0 34.0 38.0 18.6 38.0 135-139 32.85 38.0 33.8 38.0 14.6 38.0 140-144 32.12925 38.0 33.4 38.0 13.8 38.0 145-149 30.562750000000005 36.6 31.0 38.0 4.2 38.0 150-151 26.274 33.5 16.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 1.0 4 0.0 5 0.0 6 0.0 7 1.0 8 0.0 9 2.0 10 2.0 11 1.0 12 2.0 13 2.0 14 4.0 15 6.0 16 3.0 17 5.0 18 12.0 19 12.0 20 21.0 21 15.0 22 13.0 23 29.0 24 28.0 25 31.0 26 37.0 27 41.0 28 52.0 29 73.0 30 81.0 31 99.0 32 108.0 33 159.0 34 232.0 35 306.0 36 789.0 37 1812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.009294147199196 19.643305702084906 15.398141170560162 41.94925898015574 2 21.871081013293203 20.441434662653624 44.36919989967394 13.31828442437923 3 16.223671013039116 25.952858575727184 38.26479438314945 19.55867602808425 4 20.431185760842315 30.809726748558536 29.330659313111056 19.42842817748809 5 22.72385252069225 35.59066967644846 27.890644594933534 13.794833207925757 6 19.412355600200904 35.78603716725264 29.206428930185837 15.595178302360624 7 16.90954773869347 19.72361809045226 46.40703517587939 16.959798994974875 8 19.060773480662984 23.606228026117527 35.48468106479156 21.848317428427926 9 20.723436322532027 19.69354433559407 39.688520472243155 19.89449886963075 10-14 21.57109111926421 28.119817057847918 31.160476453736745 19.148615369151127 15-19 22.86734027044689 26.33589704921329 31.68451213994873 19.11225054039109 20-24 22.25292669446817 26.06139777922926 32.33180927498367 19.353866251318898 25-29 22.881696652929943 25.791536837873153 31.98311388079204 19.343652628404865 30-34 23.38458446387298 26.32901215958195 31.20791880213044 19.07848457441463 35-39 22.91457286432161 26.969849246231153 30.256281407035175 19.859296482412063 40-44 21.653365457009485 27.6665160869347 31.004366812227076 19.67575164382874 45-49 21.451880114463577 28.214267784527337 31.542748129926203 18.791103971082887 50-54 22.093373493975903 27.03313253012048 30.878514056224898 19.994979919678716 55-59 20.959076073311575 27.416520210896312 30.93648004017073 20.68792367562139 60-64 21.64291449217182 27.253111200321158 31.202328382175832 19.901645925331195 65-69 22.115770465489568 26.80577849117175 30.843699839486355 20.234751203852326 70-74 21.99709112794022 26.53593460053162 31.430864135613625 20.03611013591454 75-79 22.491488083316643 25.84618465852193 31.27378329661526 20.388543961546166 80-84 22.501127310987524 25.978255423618418 32.41144345909114 19.10917380630292 85-89 22.035429317007075 27.575651126612133 30.23535906057108 20.153560495809707 90-94 22.497114467807496 26.898178350981084 30.33070708084508 20.274000100366337 95-99 21.69152729646929 27.38184922906936 30.681532821053686 20.24509065340766 100-104 22.515192607101604 26.824368439556025 31.083320777459694 19.577118175882678 105-109 24.327038971474487 26.175170751305743 30.263157894736842 19.234632382482925 110-114 23.1271339626431 25.883711588672426 31.02530628640289 19.963848162281582 115-119 22.97962052002811 27.186025499447847 30.398554362011847 19.4357996185122 120-124 22.399518289929247 27.949219730041648 28.897586431833005 20.753675548196096 125-129 23.44063377456879 27.737665463297233 29.226835138387486 19.59486562374649 130-134 23.06997742663657 28.623024830699773 29.370453975420112 18.936543767243542 135-139 24.05279269333066 28.087519445977822 29.146384302704874 18.713303557986652 140-144 24.028091296714322 28.48758465011287 28.9791823426135 18.505141710559318 145-149 24.623645122440788 27.393617021276594 28.77358490566038 19.20915295062224 150-151 25.691749092274947 27.507199198697883 29.13484412169776 17.66620758732941 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 2.0 2 0.5 3 0.5 4 1.0 5 2.5 6 2.0 7 0.5 8 0.5 9 1.0 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.0 20 1.5 21 2.5 22 6.0 23 12.0 24 16.0 25 21.5 26 26.0 27 32.5 28 41.5 29 50.0 30 61.0 31 68.0 32 66.5 33 79.5 34 115.5 35 158.5 36 162.0 37 190.0 38 214.5 39 205.0 40 236.0 41 256.5 42 240.0 43 221.5 44 216.0 45 196.5 46 167.5 47 133.0 48 108.0 49 94.5 50 77.5 51 53.0 52 37.0 53 36.5 54 29.5 55 20.5 56 20.0 57 18.0 58 20.0 59 27.0 60 35.0 61 32.0 62 23.0 63 31.5 64 30.5 65 24.5 66 20.0 67 10.5 68 9.5 69 7.5 70 4.0 71 4.0 72 5.5 73 4.5 74 2.0 75 0.5 76 0.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.475 2 0.325 3 0.3 4 0.27499999999999997 5 0.325 6 0.44999999999999996 7 0.5 8 0.44999999999999996 9 0.475 10-14 0.515 15-19 0.5349999999999999 20-24 0.485 25-29 0.51 30-34 0.49 35-39 0.5 40-44 0.385 45-49 0.40499999999999997 50-54 0.4 55-59 0.42500000000000004 60-64 0.36 65-69 0.32 70-74 0.305 75-79 0.13999999999999999 80-84 0.20500000000000002 85-89 0.365 90-94 0.365 95-99 0.445 100-104 0.445 105-109 0.44 110-114 0.42 115-119 0.38999999999999996 120-124 0.35500000000000004 125-129 0.27999999999999997 130-134 0.325 135-139 0.365 140-144 0.325 145-149 0.36 150-151 0.1625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.7 #Duplication Level Percentage of deduplicated Percentage of total 1 86.27922971114168 62.724999999999994 2 6.980742778541953 10.15 3 2.8198074277854195 6.15 4 1.2723521320495186 3.6999999999999997 5 0.4470426409903714 1.625 6 0.5845942228335627 2.55 7 0.30949105914718017 1.575 8 0.30949105914718017 1.7999999999999998 9 0.30949105914718017 2.025 >10 0.6877579092159559 7.7 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 40 1.0 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 27 0.675 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 22 0.5499999999999999 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 20 0.5 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 18 0.44999999999999996 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 18 0.44999999999999996 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 15 0.375 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 13 0.325 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 13 0.325 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 12 0.3 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 12 0.3 No Hit CGTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGAC 11 0.27499999999999997 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 11 0.27499999999999997 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 11 0.27499999999999997 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11 0.27499999999999997 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 10 0.25 No Hit GTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACTGAATTTAAAATG 10 0.25 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 9 0.22499999999999998 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 9 0.22499999999999998 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 9 0.22499999999999998 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 9 0.22499999999999998 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 9 0.22499999999999998 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 9 0.22499999999999998 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 9 0.22499999999999998 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 8 0.2 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 8 0.2 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 8 0.2 No Hit CTTTGGTACAAAATTGACAATCTCACAAGGATGAAATACCAGTAATTTTT 8 0.2 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 8 0.2 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 8 0.2 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 7 0.17500000000000002 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 7 0.17500000000000002 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 7 0.17500000000000002 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 7 0.17500000000000002 No Hit CTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAACCATTTATGCG 7 0.17500000000000002 No Hit CTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGT 7 0.17500000000000002 No Hit CTGTTCTATAGGATCGTACCGCTACATCCTTTACCAAAAAGGAGGCAAGA 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit GTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTT 6 0.15 No Hit CTATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCA 6 0.15 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 6 0.15 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 6 0.15 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 6 0.15 No Hit CTCAGTTCGGTAGAGCAACTCGTTTACACGTGCGCCAATGCTTTTCAAAG 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 6 0.15 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 6 0.15 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 6 0.15 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 6 0.15 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 5 0.125 No Hit CGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTAT 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit CTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGTTGGCTGTCTAATTG 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 5 0.125 No Hit TATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATG 5 0.125 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.0875 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.1125 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.15 0.0 0.0 0.0 0.0 94-95 0.225 0.0 0.0 0.0 0.0 96-97 0.3125 0.0 0.0 0.0 0.0 98-99 0.5125 0.0 0.0 0.0 0.0 100-101 0.725 0.0 0.0 0.0 0.0 102-103 0.9125 0.0 0.0 0.0 0.0 104-105 1.0499999999999998 0.0 0.0 0.0 0.0 106-107 1.4500000000000002 0.0 0.0 0.0 0.0 108-109 1.925 0.0 0.0 0.0 0.0 110-111 2.4000000000000004 0.0 0.0 0.0 0.0 112-113 2.65 0.0 0.0 0.0 0.0 114-115 3.125 0.0 0.0 0.0 0.0 116-117 3.5 0.0 0.0 0.0 0.0 118-119 3.9375 0.0 0.0 0.0 0.0 120-121 4.487500000000001 0.0 0.0 0.0 0.0 122-123 5.1375 0.0 0.0 0.0 0.0 124-125 5.6125 0.0 0.0 0.0 0.0 126-127 6.2875 0.0 0.0 0.0 0.0 128-129 7.0 0.0 0.0 0.0 0.0 130-131 7.9 0.0 0.0 0.0 0.0 132-133 8.6125 0.0 0.0 0.0 0.0 134-135 9.225 0.0 0.0 0.0 0.0 136-137 9.8125 0.0 0.0 0.0 0.0 138-139 10.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755520 spots for SRR8846510.sra Written 1755520 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra Read 1755512 spots for SRR8846510.sra Written 1755512 spots for SRR8846510.sra SRR ids: ['SRR8846510.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_n9rc3op1 SRR8846510.sra spots: 35110248 blocks: [[1, 1755512], [1755513, 3511024], [3511025, 5266536], [5266537, 7022048], [7022049, 8777560], [8777561, 10533072], [10533073, 12288584], [12288585, 14044096], [14044097, 15799608], [15799609, 17555120], [17555121, 19310632], [19310633, 21066144], [21066145, 22821656], [22821657, 24577168], [24577169, 26332680], [26332681, 28088192], [28088193, 29843704], [29843705, 31599216], [31599217, 33354728], [33354729, 35110248]] SRR8846510 file size 11876010 SRR8846510 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846510 SRR8846510_1.fastq SRR8846510_2.fastq Input file: SRR8846510_1.fastq Paired file: SRR8846510_2.fastq trimmed: SRR8846510-trimmed-pair1.fastq, SRR8846510-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 04:15:18 2024 >> started Mon Dec 9 04:18:36 2024 >> done (198.040s) 35110248 read pairs processed; of these: 13991 ( 0.04%) short read pairs filtered out after trimming by size control 107632 ( 0.31%) empty read pairs filtered out after trimming by size control 34988625 (99.65%) read pairs available; of these: 15682497 (44.82%) trimmed read pairs available after processing 19306128 (55.18%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 9 0.00% 20 7 0.00% 21 7 0.00% 22 9 0.00% 23 7 0.00% 24 5 0.00% 25 10 0.00% 26 12 0.00% 27 26 0.00% 28 12 0.00% 29 9 0.00% 30 13 0.00% 31 10 0.00% 32 10 0.00% 33 13 0.00% 34 19 0.00% 35 23 0.00% 36 20 0.00% 37 20 0.00% 38 26 0.00% 39 26 0.00% 40 32 0.00% 41 32 0.00% 42 37 0.00% 43 60 0.00% 44 53 0.00% 45 46 0.00% 46 63 0.00% 47 62 0.00% 48 83 0.00% 49 94 0.00% 50 111 0.00% 51 129 0.00% 52 124 0.00% 53 145 0.00% 54 167 0.00% 55 195 0.00% 56 216 0.00% 57 252 0.00% 58 300 0.00% 59 343 0.00% 60 332 0.00% 61 412 0.00% 62 491 0.00% 63 558 0.00% 64 623 0.00% 65 731 0.00% 66 821 0.00% 67 807 0.00% 68 1059 0.00% 69 1212 0.00% 70 1203 0.00% 71 1436 0.00% 72 1636 0.00% 73 1750 0.01% 74 2135 0.01% 75 2623 0.01% 76 2694 0.01% 77 3152 0.01% 78 3384 0.01% 79 3609 0.01% 80 4211 0.01% 81 4847 0.01% 82 5655 0.02% 83 6371 0.02% 84 7213 0.02% 85 9226 0.03% 86 10031 0.03% 87 10701 0.03% 88 11551 0.03% 89 12149 0.03% 90 14795 0.04% 91 15228 0.04% 92 17837 0.05% 93 19855 0.06% 94 22591 0.06% 95 24069 0.07% 96 25476 0.07% 97 26891 0.08% 98 31306 0.09% 99 33669 0.10% 100 35958 0.10% 101 39419 0.11% 102 43536 0.12% 103 43917 0.13% 104 49666 0.14% 105 57847 0.17% 106 61408 0.18% 107 63909 0.18% 108 65196 0.19% 109 75816 0.22% 110 73791 0.21% 111 81861 0.23% 112 82041 0.23% 113 77408 0.22% 114 81204 0.23% 115 87071 0.25% 116 95669 0.27% 117 101530 0.29% 118 101253 0.29% 119 111983 0.32% 120 111822 0.32% 121 116465 0.33% 122 119134 0.34% 123 119363 0.34% 124 129167 0.37% 125 146656 0.42% 126 136675 0.39% 127 148088 0.42% 128 157492 0.45% 129 172815 0.49% 130 162862 0.47% 131 186149 0.53% 132 169541 0.48% 133 174614 0.50% 134 182202 0.52% 135 181765 0.52% 136 196592 0.56% 137 197026 0.56% 138 219173 0.63% 139 223866 0.64% 140 230886 0.66% 141 267451 0.76% 142 246745 0.71% 143 277810 0.79% 144 303341 0.87% 145 365963 1.05% 146 380726 1.09% 147 474363 1.36% 148 653468 1.87% 149 1184025 3.38% 150 6268578 17.92% 151 19306128 55.18% 34988625 reads passed initial QC criterion=sequence-density sequence-density=3.31 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=34 prefix-density=3.21 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=1162.85 fanout-score-rank=1 prefix-density=11.55 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.76 sequence-density-rank=1 fanout-score=1.86 fanout-score-rank=30 prefix-density=3.24 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=170.20 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=3.6 sequence=AAAGGAAAATGGGGATATGGCGAAATCGGTAGACGCTACGGACTTGATTGTATTGAGCCTTAGTATGGAAACCTGCTAAGTGTTAACTTCCAAATTCAGAGAAACCCTGGAATTAAAAAAGGGCAATCCTGAGCCAAATCCGTGTTTTGAGAAAACAAGGGGTTCTCGAACTAGAATCCAAAGGAAAAGGATAGGTGCAGAGACTCAATGGAAGCTGTTCTAACGAATCGAGTTAATTTATTTAGGTTGTTTTGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x CCAGCCTCACGC -y TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG -o SRR8846510 SRR8846510_1.fastq SRR8846510_2.fastq Input file: SRR8846510_1.fastq Paired file: SRR8846510_2.fastq trimmed: SRR8846510-trimmed-pair1.fastq, SRR8846510-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): CCAGCCTCACGC -- paired 3' end adapter sequence (-y): TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 04:34:34 2024 >> started Mon Dec 9 04:35:41 2024 >> done (66.233s) 11662875 read pairs processed; of these: 1764 ( 0.02%) short read pairs filtered out after trimming by size control 4812 ( 0.04%) empty read pairs filtered out after trimming by size control 11656299 (99.94%) read pairs available; of these: 1866 ( 0.02%) trimmed read pairs available after processing 11654433 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 3 0.00% 20 5 0.00% 21 1 0.00% 22 4 0.00% 23 2 0.00% 24 2 0.00% 25 3 0.00% 26 3 0.00% 27 11 0.00% 28 4 0.00% 29 5 0.00% 30 3 0.00% 31 0 0.00% 32 5 0.00% 33 4 0.00% 34 10 0.00% 35 12 0.00% 36 7 0.00% 37 7 0.00% 38 4 0.00% 39 9 0.00% 40 12 0.00% 41 10 0.00% 42 14 0.00% 43 19 0.00% 44 19 0.00% 45 18 0.00% 46 25 0.00% 47 20 0.00% 48 20 0.00% 49 30 0.00% 50 46 0.00% 51 43 0.00% 52 40 0.00% 53 50 0.00% 54 54 0.00% 55 62 0.00% 56 63 0.00% 57 88 0.00% 58 99 0.00% 59 113 0.00% 60 114 0.00% 61 128 0.00% 62 161 0.00% 63 187 0.00% 64 217 0.00% 65 215 0.00% 66 275 0.00% 67 268 0.00% 68 348 0.00% 69 415 0.00% 70 379 0.00% 71 490 0.00% 72 528 0.00% 73 598 0.01% 74 688 0.01% 75 833 0.01% 76 889 0.01% 77 1106 0.01% 78 1150 0.01% 79 1213 0.01% 80 1418 0.01% 81 1638 0.01% 82 1871 0.02% 83 2113 0.02% 84 2375 0.02% 85 3120 0.03% 86 3391 0.03% 87 3547 0.03% 88 3885 0.03% 89 3966 0.03% 90 4858 0.04% 91 5082 0.04% 92 5887 0.05% 93 6657 0.06% 94 7529 0.06% 95 8082 0.07% 96 8460 0.07% 97 8976 0.08% 98 10355 0.09% 99 11224 0.10% 100 12166 0.10% 101 13015 0.11% 102 14441 0.12% 103 14626 0.13% 104 16519 0.14% 105 19353 0.17% 106 20405 0.18% 107 21405 0.18% 108 21792 0.19% 109 25201 0.22% 110 24354 0.21% 111 27320 0.23% 112 27439 0.24% 113 25861 0.22% 114 27109 0.23% 115 29177 0.25% 116 32031 0.27% 117 33868 0.29% 118 33679 0.29% 119 37051 0.32% 120 37242 0.32% 121 38980 0.33% 122 39877 0.34% 123 40128 0.34% 124 42741 0.37% 125 48767 0.42% 126 45571 0.39% 127 49638 0.43% 128 52303 0.45% 129 57729 0.50% 130 53902 0.46% 131 61946 0.53% 132 56425 0.48% 133 58080 0.50% 134 60773 0.52% 135 60929 0.52% 136 65294 0.56% 137 66252 0.57% 138 72772 0.62% 139 74641 0.64% 140 76855 0.66% 141 88870 0.76% 142 82149 0.70% 143 92304 0.79% 144 101214 0.87% 145 121933 1.05% 146 126959 1.09% 147 157871 1.35% 148 217759 1.87% 149 395219 3.39% 150 2087926 17.91% 151 6430851 55.17% criterion=sequence-density sequence-density=3.22 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=36 prefix-density=3.18 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=1306.03 fanout-score-rank=1 prefix-density=11.67 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.76 sequence-density-rank=1 fanout-score=1.83 fanout-score-rank=30 prefix-density=3.19 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=74.55 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=5.7 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR8846510 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 04:41:37 Started mapping on | Dec 09 04:41:38 Finished on | Dec 09 04:54:35 Mapping speed, Million of reads per hour | 162.08 Number of input reads | 34982049 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 21770790 Uniquely mapped reads % | 62.23% Average mapped length | 292.93 Number of splices: Total | 4532090 Number of splices: Annotated (sjdb) | 4062105 Number of splices: GT/AG | 4345605 Number of splices: GC/AG | 50064 Number of splices: AT/AC | 16655 Number of splices: Non-canonical | 119766 Mismatch rate per base, % | 0.21% Deletion rate per base | 0.01% Deletion average length | 1.67 Insertion rate per base | 0.02% Insertion average length | 1.76 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11931061 % of reads mapped to multiple loci | 34.11% Number of reads mapped to too many loci | 4386 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.21% % of reads unmapped: other | 0.43% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1287609 1287609 1287609 N_multimapping 11931061 11931061 11931061 N_noFeature 5730509 20805957 6174876 N_ambiguous 1085042 45633 562530 UnstrandedReadsAssigned:14955239 PositiveStrandReadsAssigned:919200 NegativeStrandReadsAssigned:15033384 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=147 echo kmer=143 SRR8846510 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846510-trimmed-pair1.fastq SRR8846510-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 34,982,049 reads, 20,376,207 reads pseudoaligned [quant] estimated average fragment length: 217.646 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,076 rounds 52973 SRR8846510.ke.tsv 35125 SRR8846510.se.tsv 88098 total ==> SRR8846510.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 720.199 0 0 PNS24247 1044 827.354 9.178 0.568755 PNS24249 1928 1711.35 22.8751 0.685317 PNS24246 1044 827.354 9.178 0.568755 PNS24248 1044 827.354 9.178 0.568755 PNS24244 1471 1254.35 40.5909 1.65912 PNS24243 293 109.774 0 0 KQK14069 1603 1386.35 1398.11 51.7052 KQK14071 474 266.412 10.4974 2.02021 ==> SRR8846510.se.tsv <== BRADI_1g14170v3 1587 BRADI_1g53295v3 30 BRADI_1g59795v3 33 BRADI_1g07683v3 0 BRADI_1g00485v3 11 BRADI_1g20270v3 337 BRADI_1g74790v3 45 BRADI_1g09890v3 1 BRADI_1g77505v3 43 BRADI_1g48960v3 0 SRR8846510 completed mapping pipeline successfully