Starting /dee2/code/volunteer_pipeline.sh SRR8846514 current disk space = 1515381104640 free memory = 1570811776 SRR8846514 SRAfilesize 37f01dc32d35b7351f67f1fbad890287 SRR8846514.sra SRR8846514.sra file validated SRR8846514 is paired end SRR8846514 is conventional basespace SRR8846514 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846514_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.264 34.0 33.0 34.0 33.0 34.0 2 33.2885 34.0 33.0 34.0 33.0 34.0 3 33.31425 34.0 33.0 34.0 33.0 34.0 4 33.29725 34.0 33.0 34.0 33.0 34.0 5 33.233 34.0 33.0 34.0 32.0 34.0 6 36.72725 38.0 37.0 38.0 34.0 38.0 7 37.128 38.0 38.0 38.0 36.0 38.0 8 37.23425 38.0 38.0 38.0 36.0 38.0 9 37.291 38.0 38.0 38.0 37.0 38.0 10-14 37.25775 38.0 38.0 38.0 36.8 38.0 15-19 37.30375 38.0 38.0 38.0 37.0 38.0 20-24 37.34805 38.0 38.0 38.0 37.0 38.0 25-29 37.2784 38.0 38.0 38.0 36.8 38.0 30-34 37.21725 38.0 38.0 38.0 36.2 38.0 35-39 37.197700000000005 38.0 38.0 38.0 36.0 38.0 40-44 37.148450000000004 38.0 38.0 38.0 36.0 38.0 45-49 37.14704999999999 38.0 38.0 38.0 36.0 38.0 50-54 37.0658 38.0 38.0 38.0 36.0 38.0 55-59 36.6263 38.0 38.0 38.0 35.2 38.0 60-64 35.7257 38.0 38.0 38.0 33.6 38.0 65-69 36.5416 38.0 38.0 38.0 34.2 38.0 70-74 36.88695 38.0 38.0 38.0 35.0 38.0 75-79 36.85725 38.0 38.0 38.0 35.2 38.0 80-84 36.6904 38.0 38.0 38.0 34.8 38.0 85-89 36.5375 38.0 38.0 38.0 34.0 38.0 90-94 36.450750000000006 38.0 38.0 38.0 34.0 38.0 95-99 36.32684999999999 38.0 38.0 38.0 34.0 38.0 100-104 36.1858 38.0 38.0 38.0 33.6 38.0 105-109 36.098850000000006 38.0 37.2 38.0 33.0 38.0 110-114 35.89615 38.0 37.0 38.0 32.2 38.0 115-119 35.79965 38.0 37.0 38.0 31.8 38.0 120-124 35.57795 38.0 36.6 38.0 31.0 38.0 125-129 35.100350000000006 38.0 35.4 38.0 28.4 38.0 130-134 34.948449999999994 38.0 35.4 38.0 27.6 38.0 135-139 34.47745 38.0 34.8 38.0 25.4 38.0 140-144 33.9254 38.0 33.2 38.0 23.4 38.0 145-149 33.35665 38.0 33.0 38.0 19.8 38.0 150-151 29.18375 35.5 27.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 3.0 18 4.0 19 1.0 20 4.0 21 7.0 22 2.0 23 6.0 24 13.0 25 17.0 26 18.0 27 30.0 28 40.0 29 43.0 30 55.0 31 76.0 32 93.0 33 136.0 34 212.0 35 323.0 36 555.0 37 2356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.925 9.9 9.825000000000001 57.35 2 17.75 13.025 43.925 25.3 3 15.25 16.8 30.575000000000003 37.375 4 22.025 25.15 26.724999999999998 26.1 5 21.349999999999998 30.925000000000004 28.499999999999996 19.225 6 18.275 36.6 27.85 17.275 7 13.175 28.349999999999998 44.525 13.950000000000001 8 15.475 26.575 39.025 18.925 9 15.85 22.55 40.625 20.974999999999998 10-14 17.955 34.115 26.1 21.83 15-19 18.695934796739838 32.751637581879095 27.651382569128458 20.901045052252613 20-24 18.17 31.319999999999997 29.17 21.34 25-29 21.404999999999998 31.619999999999997 27.694999999999997 19.28 30-34 22.67 31.035 25.345000000000002 20.95 35-39 19.81 34.11 25.790000000000003 20.29 40-44 18.01 31.005 28.110000000000003 22.875 45-49 18.8 31.240000000000002 27.935 22.025 50-54 19.0 31.840000000000003 28.555000000000003 20.605 55-59 21.68340313871928 30.695867184740372 24.887722662360602 22.733007014179744 60-64 19.17758558185302 32.818892744316116 27.080635972862392 20.92288570096846 65-69 20.901598150567896 31.133782289677352 25.826716252889735 22.137903306865013 70-74 21.62 31.019999999999996 24.485 22.875 75-79 20.365 31.935000000000002 26.884999999999998 20.815 80-84 21.92 30.895 25.865 21.32 85-89 22.805 31.09 25.445 20.66 90-94 21.166058302915143 31.486574328716436 26.511325566278316 20.836041802090104 95-99 21.135 31.369999999999997 26.365 21.13 100-104 19.07 30.915 26.779999999999998 23.235 105-109 21.385 27.71 28.21 22.695 110-114 18.915000000000003 30.43 28.08 22.575 115-119 18.48 30.25 28.255000000000003 23.015 120-124 17.294999999999998 31.724999999999998 25.990000000000002 24.990000000000002 125-129 20.901045052252613 33.296664833241664 23.53117655882794 22.271113555677786 130-134 22.441122056102806 31.881594079703984 24.911245562278115 20.766038301915096 135-139 22.604520904180838 30.31606321264253 24.70994198839768 22.369473894778956 140-144 22.189999999999998 30.830000000000002 26.465 20.515 145-149 21.305 31.14 25.245 22.31 150-151 20.1875 31.424999999999997 25.1 23.2875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 2.0 20 3.0 21 4.0 22 7.0 23 7.5 24 11.0 25 11.5 26 16.5 27 27.5 28 32.5 29 38.0 30 41.5 31 49.0 32 58.0 33 61.5 34 84.5 35 103.5 36 148.5 37 315.5 38 346.0 39 228.0 40 223.5 41 234.0 42 219.0 43 242.5 44 241.5 45 213.0 46 178.0 47 137.0 48 115.0 49 80.0 50 53.0 51 42.5 52 31.5 53 23.5 54 26.0 55 31.5 56 28.0 57 22.5 58 27.5 59 30.0 60 27.0 61 26.5 62 22.0 63 20.5 64 24.0 65 25.0 66 17.0 67 9.5 68 5.5 69 2.5 70 3.5 71 5.0 72 4.0 73 2.5 74 2.5 75 1.0 76 1.0 77 2.5 78 1.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.005 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.915 60-64 3.4549999999999996 65-69 0.51 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.005 130-134 0.005 135-139 0.02 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.525 #Duplication Level Percentage of deduplicated Percentage of total 1 84.81773769259678 56.425000000000004 2 7.966929725667042 10.6 3 2.7433295753476137 5.475 4 1.2777151446824502 3.4000000000000004 5 0.977076287110109 3.25 6 0.5261180007515971 2.1 7 0.4509582863585118 2.1 8 0.18789928598271327 1.0 9 0.18789928598271327 1.125 >10 0.7891770011273956 9.3 >50 0.03757985719654265 2.175 >100 0.03757985719654265 3.05 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 122 3.05 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 87 2.175 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 40 1.0 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 40 1.0 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 38 0.95 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 25 0.625 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 21 0.525 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 20 0.5 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 20 0.5 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 19 0.475 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 15 0.375 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 14 0.35000000000000003 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 12 0.3 No Hit CTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACTGTCT 12 0.3 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 12 0.3 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 11 0.27499999999999997 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 11 0.27499999999999997 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 11 0.27499999999999997 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 11 0.27499999999999997 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 10 0.25 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 10 0.25 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 10 0.25 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 10 0.25 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 9 0.22499999999999998 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 9 0.22499999999999998 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 9 0.22499999999999998 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 9 0.22499999999999998 No Hit GTGGTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCG 9 0.22499999999999998 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 8 0.2 No Hit CTTGGTTTCATACTCCGGGGTGTAGTAAGTCAATCTATAATCTTTAACAC 8 0.2 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 7 0.17500000000000002 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 7 0.17500000000000002 No Hit CCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGACT 7 0.17500000000000002 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 7 0.17500000000000002 No Hit ATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTG 7 0.17500000000000002 No Hit CCCAGGTTGAGGAGATACTCGGAATGCTGCCAAGATATCAGTATCCTTGG 7 0.17500000000000002 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 7 0.17500000000000002 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 7 0.17500000000000002 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 7 0.17500000000000002 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 6 0.15 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGG 6 0.15 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 6 0.15 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 6 0.15 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 6 0.15 No Hit CTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAA 6 0.15 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 6 0.15 No Hit GCCGAGTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATT 6 0.15 No Hit CCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCG 6 0.15 No Hit GTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCC 6 0.15 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 6 0.15 No Hit CTTCTAACTTACCTACTACTGTACCGGCGTGGATATGATCTCCCCCAGAC 5 0.125 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 5 0.125 No Hit CCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGAT 5 0.125 No Hit GCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 5 0.125 No Hit CTTCGGCACAAAAGACAAAACGATCTCTCCAGCGCATAAATGGTTGTGAG 5 0.125 No Hit GTCCAAACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGC 5 0.125 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 5 0.125 No Hit CCAAACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCC 5 0.125 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 5 0.125 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 5 0.125 No Hit CCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATGAT 5 0.125 No Hit CTTGATTTCACCGGTTTCCGCCTGTGATTTATAAATAGCTTCGGCACAAA 5 0.125 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit CCCAATTTTGGCTTAATAGTACATCCCAATAAAGGACGACCATACTTGTT 5 0.125 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 5 0.125 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 5 0.125 No Hit CTCTGGGCCACCCTGCGTCAGCCGGAGATGGGCAGGAACGATCCTCTACA 5 0.125 No Hit CTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTT 5 0.125 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.5 0.0 0.0 0.0 0.0 92-93 0.55 0.0 0.0 0.0 0.0 94-95 0.625 0.0 0.0 0.0 0.0 96-97 0.7749999999999999 0.0 0.0 0.0 0.0 98-99 1.0 0.0 0.0 0.0 0.0 100-101 1.175 0.0 0.0 0.0 0.0 102-103 1.3 0.0 0.0 0.0 0.0 104-105 1.5375 0.0 0.0 0.0 0.0 106-107 1.7875 0.0 0.0 0.0 0.0 108-109 2.0125 0.0 0.0 0.0 0.0 110-111 2.2875 0.0 0.0 0.0 0.0 112-113 2.6625 0.0 0.0 0.0 0.0 114-115 3.1625 0.0 0.0 0.0 0.0 116-117 3.4625 0.0 0.0 0.0 0.0 118-119 3.85 0.0 0.0 0.0 0.0 120-121 4.45 0.0 0.0 0.0 0.0 122-123 5.15 0.0 0.0 0.0 0.0 124-125 5.75 0.0 0.0 0.0 0.0 126-127 6.4 0.0 0.0 0.0 0.0 128-129 7.125 0.0 0.0 0.0 0.0 130-131 7.9625 0.0 0.0 0.0 0.0 132-133 8.8625 0.0 0.0 0.0 0.0 134-135 9.8625 0.0 0.0 0.0 0.0 136-137 10.5625 0.0 0.0 0.0 0.0 138-139 11.2375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATGTA 10 0.00686971 144.72499 4 TCAAATT 10 0.00686971 144.72499 145 TTTCTTT 35 1.4788384E-9 124.04999 2 TCTTTTC 35 1.4788384E-9 124.04999 4 CTTTCTT 35 1.4788384E-9 124.04999 1 TCAGCCC 30 9.227733E-8 120.60417 145 TTCTTTT 40 3.745299E-9 108.54375 3 CTTTTCT 55 3.4226105E-8 78.94091 5 TCTTCAA 55 3.4226105E-8 78.94091 9 TTCTTCA 60 6.258597E-8 72.3625 8 TTTCTTC 65 1.089993E-7 66.79615 7 TTTTCTT 65 1.089993E-7 66.79615 6 CTTCAAA 55 3.40921E-6 65.78409 6 GATATCA 50 1.7937113E-4 57.89 145 TCAAAAA 65 9.187588E-6 55.66346 8 TTCAAAA 70 1.4253836E-5 51.687496 7 CAAAAAT 70 1.4253836E-5 51.687496 9 GGAACTT 55 1.382432E-10 29.68718 55-59 TAGCGGG 55 1.7644197E-10 29.090452 50-54 TTAGCGG 55 1.8735591E-10 28.945002 25-29 >>END_MODULE SRR8846514 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846514_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5615 33.0 33.0 34.0 32.0 34.0 2 32.82375 33.0 33.0 34.0 32.0 34.0 3 32.85575 33.0 33.0 34.0 32.0 34.0 4 32.7915 33.0 33.0 34.0 32.0 34.0 5 32.8555 33.0 33.0 34.0 32.0 34.0 6 36.8575 38.0 38.0 38.0 36.0 38.0 7 37.04425 38.0 38.0 38.0 36.0 38.0 8 36.94275 38.0 38.0 38.0 36.0 38.0 9 37.0185 38.0 38.0 38.0 36.0 38.0 10-14 36.940999999999995 38.0 38.0 38.0 36.0 38.0 15-19 36.89705 38.0 38.0 38.0 36.0 38.0 20-24 36.91605 38.0 38.0 38.0 36.0 38.0 25-29 36.8668 38.0 38.0 38.0 36.0 38.0 30-34 36.889199999999995 38.0 38.0 38.0 36.0 38.0 35-39 36.8504 38.0 38.0 38.0 36.0 38.0 40-44 36.801750000000006 38.0 38.0 38.0 35.4 38.0 45-49 36.796299999999995 38.0 38.0 38.0 35.6 38.0 50-54 36.68025 38.0 38.0 38.0 35.2 38.0 55-59 36.630700000000004 38.0 38.0 38.0 35.0 38.0 60-64 36.6332 38.0 38.0 38.0 34.4 38.0 65-69 36.60265 38.0 38.0 38.0 34.6 38.0 70-74 36.54245 38.0 38.0 38.0 34.2 38.0 75-79 36.508849999999995 38.0 38.0 38.0 34.2 38.0 80-84 36.471000000000004 38.0 38.0 38.0 34.0 38.0 85-89 36.37735 38.0 38.0 38.0 34.0 38.0 90-94 36.23915 38.0 38.0 38.0 34.0 38.0 95-99 36.09745 38.0 38.0 38.0 33.2 38.0 100-104 36.01965 38.0 37.4 38.0 33.2 38.0 105-109 35.79185 38.0 37.0 38.0 32.0 38.0 110-114 35.607150000000004 38.0 37.0 38.0 31.2 38.0 115-119 35.451950000000004 38.0 37.0 38.0 31.0 38.0 120-124 35.247249999999994 38.0 36.0 38.0 29.2 38.0 125-129 34.90785 38.0 36.0 38.0 28.0 38.0 130-134 34.6065 38.0 35.4 38.0 27.4 38.0 135-139 34.16414999999999 38.0 35.0 38.0 23.6 38.0 140-144 33.65495 38.0 34.8 38.0 19.2 38.0 145-149 32.32135000000001 38.0 33.6 38.0 11.0 38.0 150-151 27.721 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 1.0 4 2.0 5 0.0 6 1.0 7 0.0 8 1.0 9 0.0 10 1.0 11 1.0 12 0.0 13 1.0 14 1.0 15 3.0 16 5.0 17 8.0 18 6.0 19 8.0 20 8.0 21 6.0 22 13.0 23 16.0 24 11.0 25 19.0 26 22.0 27 24.0 28 34.0 29 46.0 30 63.0 31 73.0 32 89.0 33 124.0 34 179.0 35 274.0 36 604.0 37 2343.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.58976930792377 19.408224674022065 18.004012036108325 40.99799398194583 2 21.28725269221137 21.838216879539193 42.19884798397195 14.675682444277486 3 16.87953919358878 25.79514149762084 38.4923616328575 18.83295767593288 4 21.231847771657485 32.473710565848776 27.641462193289932 18.652979469203807 5 22.464312546957174 33.83420986726772 30.37816178312046 13.323315802654644 6 18.32080200501253 35.88972431077694 30.576441102756892 15.213032581453634 7 16.925777331995988 19.332998996990973 46.38916750250752 17.352056168505516 8 18.471177944862156 24.837092731829575 35.98997493734336 20.701754385964914 9 21.584356981699674 19.453497117071947 39.032338932063176 19.929806969165202 10-14 21.752872121607382 27.757989264034517 31.36507299453168 19.124065619826418 15-19 22.360497792051383 26.931955038137296 32.0303091128061 18.67723805700522 20-24 22.408865266008124 26.96685553828411 32.056360627789196 18.567918567918568 25-29 23.197712336326695 25.0990819244469 32.39351828625897 19.30968745296744 30-34 24.38070404172099 25.7847758499649 31.300772239494535 18.533747868819578 35-39 23.72354298324807 25.714715618417095 30.68512388404053 19.876617514294313 40-44 21.20954003407155 27.938671209540033 31.035173865116743 19.81661489127167 45-49 21.736079787500625 28.166190547787302 30.97278604721095 19.124943617501128 50-54 21.494437205572815 27.8340182419565 30.790818883431893 19.88072566903879 55-59 20.641604010025063 28.51127819548872 29.674185463659146 21.17293233082707 60-64 21.46901147352072 26.885114484693624 31.4394508742923 20.206423167493362 65-69 22.00070129740019 26.834644091569405 30.862094875519713 20.302559735510695 70-74 22.20886551465064 27.312797395442022 31.05434510393188 19.42399198597546 75-79 22.191095547773887 25.892946473236616 31.81590795397699 20.100050025012507 80-84 22.901756668835393 26.179870877333467 32.150543015865075 18.767829437966068 85-89 22.387012726726123 27.587934662791863 30.243511373885156 19.78154123659685 90-94 22.330778095094946 26.850042587303975 30.367252868380177 20.451926449220903 95-99 21.963202486589463 27.80869303654685 30.17496365368226 20.05314082318143 100-104 22.94235588972431 27.764411027568926 30.466165413533837 18.82706766917293 105-109 24.0 25.468671679197996 30.8671679197995 19.664160401002505 110-114 23.141697158037193 26.008721367350006 30.800461129767932 20.04912034484487 115-119 22.643683920428924 26.73748559402716 31.23715989377161 19.38167059177231 120-124 22.11923847695391 27.510020040080157 29.669338677354713 20.70140280561122 125-129 23.702924679487182 27.158453525641026 29.336939102564102 19.801682692307693 130-134 23.167860542002707 28.592896859189498 29.239092320793468 19.000150278014328 135-139 24.08056919531015 28.23429201322778 29.04098607074857 18.644152720713496 140-144 24.584251652975357 27.42436385493889 29.468042476457622 18.52334201562813 145-149 24.564128256513026 27.22444889779559 29.143286573146295 19.068136272545093 150-151 25.021894157387713 27.63668209683473 29.500813211560118 17.84061053421744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 1.0 5 1.0 6 1.0 7 1.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.0 20 4.0 21 6.0 22 6.0 23 8.5 24 8.0 25 15.5 26 24.0 27 32.5 28 42.5 29 54.5 30 62.0 31 64.5 32 65.0 33 64.5 34 112.5 35 165.0 36 152.0 37 190.5 38 242.0 39 227.5 40 242.0 41 254.0 42 243.5 43 254.5 44 244.5 45 204.5 46 170.0 47 128.5 48 82.5 49 64.5 50 58.5 51 46.0 52 36.0 53 29.5 54 29.5 55 29.0 56 27.5 57 19.5 58 15.0 59 25.0 60 34.0 61 26.0 62 19.5 63 27.0 64 30.0 65 30.5 66 23.0 67 8.5 68 6.0 69 5.0 70 3.5 71 3.0 72 2.5 73 5.0 74 6.5 75 4.0 76 3.0 77 2.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.17500000000000002 3 0.17500000000000002 4 0.15 5 0.17500000000000002 6 0.25 7 0.3 8 0.25 9 0.27499999999999997 10-14 0.335 15-19 0.36 20-24 0.28500000000000003 25-29 0.335 30-34 0.29 35-39 0.31 40-44 0.21 45-49 0.23500000000000001 50-54 0.22999999999999998 55-59 0.25 60-64 0.20500000000000002 65-69 0.185 70-74 0.17500000000000002 75-79 0.05 80-84 0.095 85-89 0.21 90-94 0.20500000000000002 95-99 0.265 100-104 0.25 105-109 0.25 110-114 0.245 115-119 0.215 120-124 0.2 125-129 0.16 130-134 0.185 135-139 0.21 140-144 0.18 145-149 0.2 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.575 #Duplication Level Percentage of deduplicated Percentage of total 1 84.87243981315127 59.050000000000004 2 7.4739489759252615 10.4 3 2.5512037369744878 5.325 4 1.8684872439813154 5.2 5 0.7186489399928134 2.5 6 0.7186489399928134 3.0 7 0.46712181099532885 2.275 8 0.21559468199784404 1.2 9 0.32339202299676606 2.025 >10 0.7905138339920948 9.025 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 33 0.8250000000000001 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 31 0.775 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 30 0.75 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 21 0.525 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 19 0.475 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 18 0.44999999999999996 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 18 0.44999999999999996 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 17 0.42500000000000004 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 17 0.42500000000000004 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 14 0.35000000000000003 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 14 0.35000000000000003 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 14 0.35000000000000003 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 13 0.325 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 13 0.325 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 13 0.325 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 12 0.3 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 11 0.27499999999999997 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 11 0.27499999999999997 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 11 0.27499999999999997 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 11 0.27499999999999997 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 10 0.25 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 10 0.25 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 9 0.22499999999999998 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 9 0.22499999999999998 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 9 0.22499999999999998 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 9 0.22499999999999998 No Hit CTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAACCATTTATGCG 9 0.22499999999999998 No Hit TGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTC 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 9 0.22499999999999998 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 9 0.22499999999999998 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 8 0.2 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 8 0.2 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit CTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACT 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit ATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGC 7 0.17500000000000002 No Hit TTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGT 7 0.17500000000000002 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 7 0.17500000000000002 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 7 0.17500000000000002 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 7 0.17500000000000002 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 7 0.17500000000000002 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 7 0.17500000000000002 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 7 0.17500000000000002 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 6 0.15 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 6 0.15 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 6 0.15 No Hit CAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTT 6 0.15 No Hit TTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATAC 6 0.15 No Hit CGTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGAC 6 0.15 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit CGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATG 6 0.15 No Hit TTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGC 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6 0.15 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 6 0.15 No Hit TGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAA 5 0.125 No Hit CATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTC 5 0.125 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 5 0.125 No Hit CACGGATCCTAGAGCGGCAGGCACCGCGTGAGGCTGGTTTCACAGAGCAG 5 0.125 No Hit CTATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCA 5 0.125 No Hit AGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACC 5 0.125 No Hit CTTGGTATGGAAGTAATGCACGAACGTAATGCTCACAACTTCCCTCTAGA 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit CCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGA 5 0.125 No Hit CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCAG 5 0.125 No Hit CTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTG 5 0.125 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit CTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.025 0.0 38-39 0.0 0.0 0.0 0.025 0.0 40-41 0.0 0.0 0.0 0.025 0.0 42-43 0.0 0.0 0.0 0.025 0.0 44-45 0.0 0.0 0.0 0.025 0.0 46-47 0.0 0.0 0.0 0.025 0.0 48-49 0.0 0.0 0.0 0.025 0.0 50-51 0.0 0.0 0.0 0.025 0.0 52-53 0.0 0.0 0.0 0.025 0.0 54-55 0.0 0.0 0.0 0.025 0.0 56-57 0.0 0.0 0.0 0.025 0.0 58-59 0.0 0.0 0.0 0.025 0.0 60-61 0.0 0.0 0.0 0.025 0.0 62-63 0.0 0.0 0.0 0.025 0.0 64-65 0.0 0.0 0.0 0.025 0.0 66-67 0.025 0.0 0.0 0.025 0.0 68-69 0.037500000000000006 0.0 0.0 0.025 0.0 70-71 0.0625 0.0 0.0 0.025 0.0 72-73 0.075 0.0 0.0 0.025 0.0 74-75 0.075 0.0 0.0 0.025 0.0 76-77 0.1 0.0 0.0 0.025 0.0 78-79 0.125 0.0 0.0 0.025 0.0 80-81 0.16249999999999998 0.0 0.0 0.025 0.0 82-83 0.1875 0.0 0.0 0.025 0.0 84-85 0.25 0.0 0.0 0.025 0.0 86-87 0.3375 0.0 0.0 0.025 0.0 88-89 0.375 0.0 0.0 0.025 0.0 90-91 0.525 0.0 0.0 0.025 0.0 92-93 0.575 0.0 0.0 0.025 0.0 94-95 0.6499999999999999 0.0 0.0 0.025 0.0 96-97 0.8 0.0 0.0 0.025 0.0 98-99 1.025 0.0 0.0 0.025 0.0 100-101 1.225 0.0 0.0 0.025 0.0 102-103 1.35 0.0 0.0 0.025 0.0 104-105 1.5875 0.0 0.0 0.025 0.0 106-107 1.85 0.0 0.0 0.025 0.0 108-109 2.0875 0.0 0.0 0.025 0.0 110-111 2.3375 0.0 0.0 0.025 0.0 112-113 2.7125 0.0 0.0 0.025 0.0 114-115 3.2125 0.0 0.0 0.025 0.0 116-117 3.5250000000000004 0.0 0.0 0.025 0.0 118-119 3.9000000000000004 0.0 0.0 0.025 0.0 120-121 4.5 0.0 0.0 0.025 0.0 122-123 5.199999999999999 0.0 0.0 0.025 0.0 124-125 5.8125 0.0 0.0 0.025 0.0 126-127 6.4625 0.0 0.0 0.025 0.0 128-129 7.15 0.0 0.0 0.025 0.0 130-131 7.987500000000001 0.0 0.0 0.025 0.0 132-133 8.875 0.0 0.0 0.025 0.0 134-135 9.8875 0.0 0.0 0.025 0.0 136-137 10.587499999999999 0.0 0.0 0.025 0.0 138-139 11.287500000000001 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGC 10 0.006830828 145.0 1 GGGGGGG 20 0.00593511 29.0 125-129 TGCTGGG 40 0.0076550315 18.125 120-124 >>END_MODULE Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516877 spots for SRR8846514.sra Written 1516877 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra Read 1516874 spots for SRR8846514.sra Written 1516874 spots for SRR8846514.sra SRR ids: ['SRR8846514.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_iiephm21 SRR8846514.sra spots: 30337483 blocks: [[1, 1516874], [1516875, 3033748], [3033749, 4550622], [4550623, 6067496], [6067497, 7584370], [7584371, 9101244], [9101245, 10618118], [10618119, 12134992], [12134993, 13651866], [13651867, 15168740], [15168741, 16685614], [16685615, 18202488], [18202489, 19719362], [19719363, 21236236], [21236237, 22753110], [22753111, 24269984], [24269985, 25786858], [25786859, 27303732], [27303733, 28820606], [28820607, 30337483]] SRR8846514 file size 10258677 SRR8846514 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846514 SRR8846514_1.fastq SRR8846514_2.fastq Input file: SRR8846514_1.fastq Paired file: SRR8846514_2.fastq trimmed: SRR8846514-trimmed-pair1.fastq, SRR8846514-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 03:03:11 2024 >> started Thu Dec 12 03:03:45 2024 >> done (33.918s) 30337483 read pairs processed; of these: 11294 ( 0.04%) short read pairs filtered out after trimming by size control 90151 ( 0.30%) empty read pairs filtered out after trimming by size control 30236038 (99.67%) read pairs available; of these: 13538602 (44.78%) trimmed read pairs available after processing 16697436 (55.22%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 8 0.00% 20 3 0.00% 21 6 0.00% 22 6 0.00% 23 6 0.00% 24 2 0.00% 25 6 0.00% 26 13 0.00% 27 47 0.00% 28 8 0.00% 29 2 0.00% 30 3 0.00% 31 8 0.00% 32 4 0.00% 33 6 0.00% 34 18 0.00% 35 12 0.00% 36 14 0.00% 37 17 0.00% 38 19 0.00% 39 26 0.00% 40 21 0.00% 41 29 0.00% 42 35 0.00% 43 32 0.00% 44 26 0.00% 45 30 0.00% 46 41 0.00% 47 56 0.00% 48 71 0.00% 49 90 0.00% 50 85 0.00% 51 94 0.00% 52 91 0.00% 53 99 0.00% 54 146 0.00% 55 152 0.00% 56 171 0.00% 57 167 0.00% 58 212 0.00% 59 277 0.00% 60 246 0.00% 61 315 0.00% 62 362 0.00% 63 419 0.00% 64 488 0.00% 65 568 0.00% 66 630 0.00% 67 676 0.00% 68 761 0.00% 69 859 0.00% 70 959 0.00% 71 1024 0.00% 72 1240 0.00% 73 1349 0.00% 74 1703 0.01% 75 2050 0.01% 76 2143 0.01% 77 2304 0.01% 78 2538 0.01% 79 2817 0.01% 80 3261 0.01% 81 3659 0.01% 82 4359 0.01% 83 5036 0.02% 84 5874 0.02% 85 7195 0.02% 86 7972 0.03% 87 8467 0.03% 88 9238 0.03% 89 9842 0.03% 90 11917 0.04% 91 12227 0.04% 92 14588 0.05% 93 16520 0.05% 94 18800 0.06% 95 20036 0.07% 96 20801 0.07% 97 21721 0.07% 98 24742 0.08% 99 26967 0.09% 100 29156 0.10% 101 31894 0.11% 102 35097 0.12% 103 36121 0.12% 104 40925 0.14% 105 47339 0.16% 106 51288 0.17% 107 52351 0.17% 108 54093 0.18% 109 62843 0.21% 110 60981 0.20% 111 70012 0.23% 112 68891 0.23% 113 66667 0.22% 114 70916 0.23% 115 75115 0.25% 116 83227 0.28% 117 88628 0.29% 118 89079 0.29% 119 98113 0.32% 120 100275 0.33% 121 104512 0.35% 122 105508 0.35% 123 104672 0.35% 124 113448 0.38% 125 130338 0.43% 126 124745 0.41% 127 132981 0.44% 128 142770 0.47% 129 156148 0.52% 130 149450 0.49% 131 166631 0.55% 132 152995 0.51% 133 154432 0.51% 134 164245 0.54% 135 161087 0.53% 136 175774 0.58% 137 176093 0.58% 138 195571 0.65% 139 200257 0.66% 140 207161 0.69% 141 236595 0.78% 142 221251 0.73% 143 249486 0.83% 144 271243 0.90% 145 319078 1.06% 146 332152 1.10% 147 406851 1.35% 148 556482 1.84% 149 998019 3.30% 150 5336774 17.65% 151 16697436 55.22% 30236038 reads passed initial QC criterion=sequence-density sequence-density=2.81 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=33 prefix-density=2.73 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=1636.32 fanout-score-rank=1 prefix-density=11.59 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.58 sequence-density-rank=1 fanout-score=1.80 fanout-score-rank=34 prefix-density=2.82 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=27 fanout-score=36.48 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=7.1 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x CCAGCCTCACGC -y TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG -o SRR8846514 SRR8846514_1.fastq SRR8846514_2.fastq Input file: SRR8846514_1.fastq Paired file: SRR8846514_2.fastq trimmed: SRR8846514-trimmed-pair1.fastq, SRR8846514-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): CCAGCCTCACGC -- paired 3' end adapter sequence (-y): TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 03:05:52 2024 >> started Thu Dec 12 03:06:03 2024 >> done (11.276s) 10078679 read pairs processed; of these: 1258 ( 0.01%) short read pairs filtered out after trimming by size control 3544 ( 0.04%) empty read pairs filtered out after trimming by size control 10073877 (99.95%) read pairs available; of these: 1621 ( 0.02%) trimmed read pairs available after processing 10072256 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 3 0.00% 20 1 0.00% 21 1 0.00% 22 1 0.00% 23 1 0.00% 24 0 0.00% 25 2 0.00% 26 4 0.00% 27 16 0.00% 28 3 0.00% 29 0 0.00% 30 2 0.00% 31 4 0.00% 32 0 0.00% 33 2 0.00% 34 8 0.00% 35 3 0.00% 36 5 0.00% 37 8 0.00% 38 10 0.00% 39 6 0.00% 40 10 0.00% 41 10 0.00% 42 16 0.00% 43 10 0.00% 44 6 0.00% 45 7 0.00% 46 14 0.00% 47 21 0.00% 48 15 0.00% 49 35 0.00% 50 33 0.00% 51 29 0.00% 52 33 0.00% 53 31 0.00% 54 58 0.00% 55 56 0.00% 56 68 0.00% 57 60 0.00% 58 71 0.00% 59 91 0.00% 60 95 0.00% 61 92 0.00% 62 122 0.00% 63 146 0.00% 64 144 0.00% 65 201 0.00% 66 212 0.00% 67 225 0.00% 68 257 0.00% 69 299 0.00% 70 316 0.00% 71 326 0.00% 72 420 0.00% 73 456 0.00% 74 564 0.01% 75 682 0.01% 76 687 0.01% 77 790 0.01% 78 829 0.01% 79 931 0.01% 80 1080 0.01% 81 1216 0.01% 82 1423 0.01% 83 1660 0.02% 84 1974 0.02% 85 2409 0.02% 86 2638 0.03% 87 2855 0.03% 88 3142 0.03% 89 3307 0.03% 90 4026 0.04% 91 4016 0.04% 92 4777 0.05% 93 5539 0.05% 94 6276 0.06% 95 6811 0.07% 96 6944 0.07% 97 7319 0.07% 98 8137 0.08% 99 9066 0.09% 100 9823 0.10% 101 10629 0.11% 102 11660 0.12% 103 12023 0.12% 104 13600 0.14% 105 15709 0.16% 106 17077 0.17% 107 17368 0.17% 108 17879 0.18% 109 20863 0.21% 110 20237 0.20% 111 23204 0.23% 112 22965 0.23% 113 22158 0.22% 114 23696 0.24% 115 25057 0.25% 116 27896 0.28% 117 29431 0.29% 118 29781 0.30% 119 32697 0.32% 120 33731 0.33% 121 34994 0.35% 122 35240 0.35% 123 34887 0.35% 124 37709 0.37% 125 43117 0.43% 126 41654 0.41% 127 44487 0.44% 128 47384 0.47% 129 51723 0.51% 130 49515 0.49% 131 55802 0.55% 132 51086 0.51% 133 51398 0.51% 134 55094 0.55% 135 53817 0.53% 136 58486 0.58% 137 58986 0.59% 138 65177 0.65% 139 66914 0.66% 140 68745 0.68% 141 79074 0.78% 142 73469 0.73% 143 83109 0.82% 144 90321 0.90% 145 106452 1.06% 146 110625 1.10% 147 135850 1.35% 148 186027 1.85% 149 333111 3.31% 150 1776558 17.64% 151 5562419 55.22% criterion=sequence-density sequence-density=2.71 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=33 prefix-density=2.68 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=1318.95 fanout-score-rank=1 prefix-density=11.71 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.56 sequence-density-rank=1 fanout-score=1.75 fanout-score-rank=34 prefix-density=2.74 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=28 fanout-score=34.58 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=6.9 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR8846514 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 03:07:00 Started mapping on | Dec 12 03:07:00 Finished on | Dec 12 03:09:24 Mapping speed, Million of reads per hour | 755.78 Number of input reads | 30231236 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 17864443 Uniquely mapped reads % | 59.09% Average mapped length | 293.29 Number of splices: Total | 2994362 Number of splices: Annotated (sjdb) | 2656704 Number of splices: GT/AG | 2833421 Number of splices: GC/AG | 32780 Number of splices: AT/AC | 20400 Number of splices: Non-canonical | 107761 Mismatch rate per base, % | 0.20% Deletion rate per base | 0.01% Deletion average length | 1.69 Insertion rate per base | 0.02% Insertion average length | 1.84 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11367416 % of reads mapped to multiple loci | 37.60% Number of reads mapped to too many loci | 3734 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.84% % of reads unmapped: other | 0.45% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1005266 1005266 1005266 N_multimapping 11367416 11367416 11367416 N_noFeature 4899255 17046399 5222939 N_ambiguous 1059557 22352 576729 UnstrandedReadsAssigned:11905631 PositiveStrandReadsAssigned:795692 NegativeStrandReadsAssigned:12064775 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=146 echo kmer=141 SRR8846514 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846514-trimmed-pair1.fastq SRR8846514-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,231,236 reads, 19,115,466 reads pseudoaligned [quant] estimated average fragment length: 209.769 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,028 rounds 52973 SRR8846514.ke.tsv 35125 SRR8846514.se.tsv 88098 total ==> SRR8846514.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 728.268 0 0 PNS24247 1044 835.231 8.3206 0.546871 PNS24249 1928 1719.23 19.7511 0.630659 PNS24246 1044 835.231 8.3206 0.546871 PNS24248 1044 835.231 8.3206 0.546871 PNS24244 1471 1262.23 8.28709 0.360412 PNS24243 293 110.664 0 0 KQK14069 1603 1394.23 501.755 19.7557 KQK14071 474 272.034 51.3092 10.354 ==> SRR8846514.se.tsv <== BRADI_1g14170v3 989 BRADI_1g53295v3 11 BRADI_1g59795v3 59 BRADI_1g07683v3 0 BRADI_1g00485v3 7 BRADI_1g20270v3 366 BRADI_1g74790v3 11 BRADI_1g09890v3 1 BRADI_1g77505v3 33 BRADI_1g48960v3 0 SRR8846514 completed mapping pipeline successfully