Starting /dee2/code/volunteer_pipeline.sh SRR8846520 current disk space = 1506170159104 free memory = 1359771532 SRR8846520 SRAfilesize 6337cd64fd19b0938485db8d1a0f7cb3 SRR8846520.sra SRR8846520.sra file validated SRR8846520 is paired end SRR8846520 is conventional basespace SRR8846520 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846520_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.29525 34.0 33.0 34.0 33.0 34.0 2 33.30075 34.0 33.0 34.0 33.0 34.0 3 33.30675 34.0 33.0 34.0 33.0 34.0 4 33.18525 34.0 33.0 34.0 32.0 34.0 5 33.23525 34.0 33.0 34.0 32.0 34.0 6 36.72775 38.0 37.0 38.0 34.0 38.0 7 37.06825 38.0 38.0 38.0 36.0 38.0 8 37.1735 38.0 38.0 38.0 36.0 38.0 9 37.161 38.0 38.0 38.0 36.0 38.0 10-14 37.2033 38.0 38.0 38.0 36.2 38.0 15-19 37.25385 38.0 38.0 38.0 36.4 38.0 20-24 37.287600000000005 38.0 38.0 38.0 36.8 38.0 25-29 37.149300000000004 38.0 38.0 38.0 36.0 38.0 30-34 37.14405 38.0 38.0 38.0 36.0 38.0 35-39 37.1225 38.0 38.0 38.0 36.0 38.0 40-44 37.108 38.0 38.0 38.0 36.0 38.0 45-49 37.0783 38.0 38.0 38.0 36.0 38.0 50-54 36.985749999999996 38.0 38.0 38.0 35.8 38.0 55-59 36.50475 38.0 38.0 38.0 34.8 38.0 60-64 35.51405 38.0 38.0 38.0 32.6 38.0 65-69 36.34155 38.0 38.0 38.0 33.0 38.0 70-74 36.7981 38.0 38.0 38.0 35.0 38.0 75-79 36.74615 38.0 38.0 38.0 35.0 38.0 80-84 36.540800000000004 38.0 38.0 38.0 34.0 38.0 85-89 36.41435 38.0 38.0 38.0 34.0 38.0 90-94 36.362249999999996 38.0 38.0 38.0 34.0 38.0 95-99 36.23415 38.0 37.6 38.0 33.4 38.0 100-104 35.9905 38.0 37.0 38.0 32.6 38.0 105-109 35.93355 38.0 37.0 38.0 32.4 38.0 110-114 35.75895 38.0 37.0 38.0 31.2 38.0 115-119 35.67015 38.0 36.6 38.0 31.2 38.0 120-124 35.429649999999995 38.0 36.0 38.0 30.0 38.0 125-129 34.9332 38.0 35.2 38.0 27.6 38.0 130-134 34.608000000000004 38.0 35.0 38.0 26.0 38.0 135-139 34.0381 38.0 34.4 38.0 23.0 38.0 140-144 33.5197 38.0 33.0 38.0 20.2 38.0 145-149 32.885000000000005 38.0 33.0 38.0 15.0 38.0 150-151 28.622500000000002 35.0 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 0.0 16 4.0 17 0.0 18 5.0 19 3.0 20 3.0 21 2.0 22 9.0 23 12.0 24 10.0 25 23.0 26 27.0 27 32.0 28 45.0 29 59.0 30 56.0 31 69.0 32 106.0 33 153.0 34 194.0 35 367.0 36 626.0 37 2193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.375 10.75 8.924999999999999 55.95 2 17.974999999999998 12.475 47.949999999999996 21.6 3 16.55 17.224999999999998 31.6 34.625 4 21.425 24.8 26.950000000000003 26.825 5 21.580395098774694 30.607651912978245 28.457114278569644 19.35483870967742 6 16.85 37.125 27.85 18.175 7 13.125 28.15 43.974999999999994 14.75 8 15.950000000000001 26.224999999999998 37.55 20.275000000000002 9 15.85 23.25 38.824999999999996 22.075 10-14 18.39 33.51 26.44 21.66 15-19 19.042617046818727 31.30252100840336 28.781512605042014 20.873349339735896 20-24 18.475 30.65 29.73 21.145 25-29 20.57 30.9 28.52 20.01 30-34 22.225 31.035 25.945 20.794999999999998 35-39 20.424999999999997 32.2 26.205000000000002 21.17 40-44 18.35 30.380000000000003 28.549999999999997 22.720000000000002 45-49 19.42 30.09 28.494999999999997 21.995 50-54 18.975 31.474999999999998 28.555000000000003 20.995 55-59 20.66959995954079 30.29383502756284 26.16699539776463 22.869569615131745 60-64 18.34881428052514 31.612267137149086 28.218566758341552 21.820351823984225 65-69 20.542362648420205 31.324210102636346 26.564701147112096 21.568726101831352 70-74 21.485000000000003 30.595 25.66 22.259999999999998 75-79 20.575 30.620000000000005 27.16 21.645 80-84 22.17 30.404999999999998 26.275 21.15 85-89 21.175 30.365 27.584999999999997 20.875 90-94 20.647064706470648 30.438043804380438 28.107810781078108 20.807080708070806 95-99 19.965 31.94 26.855 21.240000000000002 100-104 19.295 31.255 27.04 22.41 105-109 20.28 28.54 28.21 22.97 110-114 19.285 29.82 28.345 22.55 115-119 18.67 29.75 28.310000000000002 23.27 120-124 17.349999999999998 31.52 26.815 24.315 125-129 20.066003300165008 31.331566578328918 26.056302815140757 22.546127306365317 130-134 21.566078303915194 31.506575328766438 25.646282314115705 21.281064053202662 135-139 21.504300860172034 30.441088217643532 25.48009601920384 22.574514902980596 140-144 21.171058552927647 31.66658332916646 26.451322566128304 20.71103555177759 145-149 20.66 31.195 25.81 22.335 150-151 19.787499999999998 31.85 25.374999999999996 22.9875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 2.5 19 3.5 20 2.0 21 4.0 22 8.5 23 12.0 24 13.0 25 17.0 26 20.0 27 27.0 28 42.0 29 51.0 30 55.5 31 64.0 32 83.5 33 89.5 34 102.0 35 112.5 36 154.5 37 285.5 38 298.5 39 198.5 40 198.5 41 222.5 42 214.5 43 234.0 44 228.5 45 193.5 46 155.0 47 119.0 48 104.5 49 81.0 50 65.0 51 48.5 52 31.0 53 29.5 54 28.5 55 37.5 56 31.5 57 24.5 58 38.5 59 46.0 60 43.0 61 29.5 62 20.5 63 21.0 64 28.5 65 34.0 66 19.0 67 6.0 68 3.0 69 2.0 70 3.0 71 2.5 72 2.0 73 1.0 74 0.5 75 1.5 76 2.0 77 1.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.025 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.04 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.135 60-64 3.6450000000000005 65-69 0.62 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.01 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.005 130-134 0.005 135-139 0.02 140-144 0.005 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.75 #Duplication Level Percentage of deduplicated Percentage of total 1 86.46048109965636 62.9 2 7.972508591065291 11.600000000000001 3 2.1649484536082473 4.725 4 1.3058419243986255 3.8 5 0.4467353951890034 1.625 6 0.48109965635738833 2.1 7 0.4123711340206186 2.1 8 0.06872852233676977 0.4 9 0.10309278350515465 0.675 >10 0.5154639175257731 6.0249999999999995 >50 0.06872852233676977 4.05 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 85 2.125 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 77 1.925 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 32 0.8 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 32 0.8 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 22 0.5499999999999999 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 20 0.5 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 17 0.42500000000000004 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 17 0.42500000000000004 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 14 0.35000000000000003 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 12 0.3 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 12 0.3 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 12 0.3 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 11 0.27499999999999997 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 10 0.25 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 10 0.25 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 10 0.25 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 9 0.22499999999999998 No Hit CTTTTTTTGATTGTCTGTCAATCAATATTCTAATGGCAATGCAATTTCAT 9 0.22499999999999998 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 9 0.22499999999999998 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 8 0.2 No Hit GTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCC 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 7 0.17500000000000002 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 7 0.17500000000000002 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 7 0.17500000000000002 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 7 0.17500000000000002 No Hit CCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGACT 7 0.17500000000000002 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 7 0.17500000000000002 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 7 0.17500000000000002 No Hit CATTCATCAACGGATGCAGCTTCCCAAATTGGGTAAAAGTGCAATCCGAT 7 0.17500000000000002 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 7 0.17500000000000002 No Hit CGGTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTTCA 7 0.17500000000000002 No Hit CTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTT 7 0.17500000000000002 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 7 0.17500000000000002 No Hit CAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCA 6 0.15 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 6 0.15 No Hit CTCGGTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTT 6 0.15 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 6 0.15 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 6 0.15 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 6 0.15 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 6 0.15 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 6 0.15 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 6 0.15 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 6 0.15 No Hit CTCTAATTCAAAACCGAACATGAAATTTTCATTTCATTCGGCTCCTTTAT 6 0.15 No Hit GTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGG 6 0.15 No Hit GTGGTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCG 6 0.15 No Hit TGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAA 5 0.125 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit CTCGTATCATTTAGGACTTTCGAATTGGAAGGAATTCTGACCCCCGGATT 5 0.125 No Hit GGGTAAACCACCGCCTCTCAGGCCTCCCCGACGGGTTCTACCATAGAGGC 5 0.125 No Hit GTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTTCAAA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit CATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATATCTACT 5 0.125 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 5 0.125 No Hit CATCCATAGATCCTTTACTCATATTTTTAATATATGGAATACTTAATCCA 5 0.125 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 5 0.125 No Hit CTTGGAAGGCTAGGGGTTATAGTCGACGTTGGTTGATTATTTATAACGTC 5 0.125 No Hit CTACGATCCAACCAATTGGGAGAGAATCAATAGACTCCTTTTCGGGAGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.0625 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.2375 0.0 0.0 0.0 0.0 94-95 0.275 0.0 0.0 0.0 0.0 96-97 0.3375 0.0 0.0 0.0 0.0 98-99 0.4125 0.0 0.0 0.0 0.0 100-101 0.5125 0.0 0.0 0.0 0.0 102-103 0.7125 0.0 0.0 0.0 0.0 104-105 0.9125 0.0 0.0 0.0 0.0 106-107 1.075 0.0 0.0 0.0 0.0 108-109 1.4125 0.0 0.0 0.0 0.0 110-111 1.7125 0.0 0.0 0.0 0.0 112-113 2.0625 0.0 0.0 0.0 0.0 114-115 2.4749999999999996 0.0 0.0 0.0 0.0 116-117 2.95 0.0 0.0 0.0 0.0 118-119 3.3375 0.0 0.0 0.0 0.0 120-121 3.8874999999999997 0.0 0.0 0.0 0.0 122-123 4.5375 0.0 0.0 0.0 0.0 124-125 5.2375 0.0 0.0 0.0 0.0 126-127 5.8 0.0 0.0 0.0 0.0 128-129 6.2 0.0 0.0 0.0 0.0 130-131 6.725 0.0 0.0 0.0 0.0 132-133 7.5 0.0 0.0 0.0 0.0 134-135 8.0875 0.0 0.0 0.0 0.0 136-137 8.95 0.0 0.0 0.0 0.0 138-139 9.7625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGAAT 10 0.0069178343 144.3875 3 AATTTCA 10 0.0069178343 144.3875 4 AATTCAA 10 0.0069178343 144.3875 5 GCGAATA 10 0.0069178343 144.3875 4 TCGCGAA 10 0.0069178343 144.3875 2 CGAATAC 10 0.0069178343 144.3875 5 GAATACC 10 0.0069178343 144.3875 6 AATACCA 10 0.0069178343 144.3875 7 CTCGCGA 10 0.0069178343 144.3875 1 CTTTCTT 20 3.6486445E-4 108.29063 1 TCTTCAA 45 0.008658382 48.738396 9 TTTCTTC 45 0.009108417 48.129166 6 TTCTTCA 45 0.009108417 48.129166 7 TCTTTTC 45 0.009108417 48.129166 3 TTCAAGA 80 0.0015548168 27.072657 7 TTCAAAA 55 1.0935634E-4 26.252272 7 AATTCTT 45 2.520872E-5 22.51657 15-19 TGTTAGC 45 2.570543E-5 22.460278 25-29 AGCGGAA 45 2.570543E-5 22.460278 30-34 CTTATAT 45 2.570543E-5 22.460278 20-24 >>END_MODULE SRR8846520 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846520_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73675 33.0 33.0 34.0 32.0 34.0 2 32.9115 33.0 33.0 34.0 32.0 34.0 3 32.9295 33.0 33.0 34.0 32.0 34.0 4 33.0005 33.0 33.0 34.0 32.0 34.0 5 32.938 33.0 33.0 34.0 32.0 34.0 6 37.10975 38.0 38.0 38.0 36.0 38.0 7 37.20175 38.0 38.0 38.0 37.0 38.0 8 37.1265 38.0 38.0 38.0 36.0 38.0 9 37.173 38.0 38.0 38.0 37.0 38.0 10-14 37.1362 38.0 38.0 38.0 36.2 38.0 15-19 37.0755 38.0 38.0 38.0 36.0 38.0 20-24 37.09275 38.0 38.0 38.0 36.2 38.0 25-29 37.03435 38.0 38.0 38.0 36.0 38.0 30-34 36.97265 38.0 38.0 38.0 36.0 38.0 35-39 36.947050000000004 38.0 38.0 38.0 36.0 38.0 40-44 36.9677 38.0 38.0 38.0 36.0 38.0 45-49 36.9833 38.0 38.0 38.0 35.8 38.0 50-54 36.830200000000005 38.0 38.0 38.0 35.6 38.0 55-59 36.76035 38.0 38.0 38.0 35.0 38.0 60-64 36.71855 38.0 38.0 38.0 34.8 38.0 65-69 36.7017 38.0 38.0 38.0 34.8 38.0 70-74 36.7276 38.0 38.0 38.0 35.0 38.0 75-79 36.6446 38.0 38.0 38.0 34.6 38.0 80-84 36.5413 38.0 38.0 38.0 34.2 38.0 85-89 36.44870000000001 38.0 38.0 38.0 34.0 38.0 90-94 36.281850000000006 38.0 38.0 38.0 34.0 38.0 95-99 36.1812 38.0 38.0 38.0 33.6 38.0 100-104 36.0834 38.0 37.2 38.0 33.0 38.0 105-109 35.8654 38.0 37.0 38.0 32.2 38.0 110-114 35.72895 38.0 37.0 38.0 31.8 38.0 115-119 35.68615 38.0 37.0 38.0 31.4 38.0 120-124 35.45495 38.0 36.2 38.0 30.6 38.0 125-129 35.20205 38.0 36.0 38.0 29.0 38.0 130-134 34.84845 38.0 35.2 38.0 27.8 38.0 135-139 34.3465 38.0 35.0 38.0 24.6 38.0 140-144 33.87055 38.0 35.0 38.0 22.6 38.0 145-149 32.69975 38.0 33.6 38.0 11.4 38.0 150-151 28.11525 35.0 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 1.0 4 0.0 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 2.0 12 2.0 13 0.0 14 1.0 15 0.0 16 2.0 17 3.0 18 1.0 19 5.0 20 7.0 21 6.0 22 8.0 23 13.0 24 15.0 25 14.0 26 25.0 27 32.0 28 36.0 29 41.0 30 69.0 31 81.0 32 89.0 33 129.0 34 170.0 35 274.0 36 599.0 37 2367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.034551827741613 20.15523284927391 15.523284927391087 41.28693039559339 2 20.45568352528793 21.231847771657485 45.81872809213821 12.493740610916374 3 15.623435152729092 25.88883324987481 39.03355032548823 19.45418127190786 4 21.357035553329993 31.146720080120183 28.392588883324986 19.103655483224838 5 22.759138708062093 34.702053079619425 28.31747621432148 14.221331997996995 6 19.954932398597897 35.57836755132699 29.86980470706059 14.596895343014522 7 17.45991983967936 19.589178356713425 45.11523046092184 17.835671342685373 8 20.58087130696044 22.633950926389584 35.202804206309466 21.58237356034051 9 20.756134201301954 20.95643465197797 37.33099649474212 20.95643465197797 10-14 21.754596923693573 28.568565559396763 30.878300516057916 18.798537000851745 15-19 22.746178902530694 26.835379604109242 31.445752944124276 18.97268854923578 20-24 22.30014025245442 26.843317972350228 31.992586655980766 18.863955119214587 25-29 23.61486824967438 25.97936078549243 31.62007814848212 18.78569281635107 30-34 23.35203366058906 26.72811059907834 31.471648968142656 18.44820677218994 35-39 22.803326320008015 26.78589319707444 30.287546338042283 20.123234144875262 40-44 21.57235853780671 27.57636454682023 31.567351026539807 19.28392588883325 45-49 21.932899349023536 29.28893340010015 30.430645968953428 18.347521281922884 50-54 22.158237356034054 27.96695042563846 30.42563845768653 19.44917376064096 55-59 20.716074111166748 28.848272408612917 29.929894842263394 20.505758637956937 60-64 21.647471206810216 27.381071607411116 31.357035553329993 19.61442163244867 65-69 22.088132198297448 27.135703555333002 30.365548322483725 20.41061592388583 70-74 22.103154732098147 26.89033550325488 31.4421632448673 19.56434651977967 75-79 21.799429229459772 27.366945376257952 31.272217493616385 19.561407900665902 80-84 22.466453034247948 26.1616262767875 32.0448628079311 19.327057881033447 85-89 21.97295943915874 27.936905358037055 29.854782173259892 20.235353029544317 90-94 22.41862794191287 27.290936404606907 30.40560841261893 19.88482724086129 95-99 21.367050575863797 27.936905358037055 30.220330495743614 20.475713570355534 100-104 22.899349023535304 27.56134201301953 30.030045067601403 19.509263895843766 105-109 23.47020530796194 26.885327991987985 30.120180270405612 19.524286429644466 110-114 22.864296444667 26.55983975963946 30.655983975963945 19.919879819729594 115-119 22.073109664496744 27.611417125688533 30.590886329494243 19.72458688032048 120-124 22.288432648973462 28.68803204807211 28.983475212819226 20.040060090135203 125-129 23.249874812218327 28.5828743114672 28.96845267901853 19.198798197295943 130-134 22.904356534802204 29.208813219829743 28.813219829744618 19.073610415623435 135-139 23.690535803705558 28.092138207310967 29.529293940911366 18.68803204807211 140-144 24.616925388082123 28.512769153730595 28.382573860791187 18.487731597396095 145-149 24.60190285428142 27.69654481722584 28.98848272408613 18.71306960440661 150-151 24.706029522141606 28.533900425318986 27.583187390542907 19.176882661996498 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 2.5 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 0.5 13 0.0 14 0.5 15 0.5 16 0.0 17 1.5 18 2.0 19 1.0 20 1.5 21 4.0 22 6.0 23 7.5 24 14.5 25 17.5 26 33.0 27 45.5 28 44.0 29 58.5 30 73.0 31 77.0 32 82.0 33 83.5 34 115.0 35 169.5 36 164.5 37 191.0 38 237.5 39 228.0 40 212.5 41 214.0 42 214.5 43 220.5 44 211.0 45 166.5 46 146.5 47 124.5 48 112.0 49 91.5 50 76.5 51 68.0 52 38.0 53 27.0 54 26.0 55 29.0 56 23.5 57 21.0 58 24.0 59 29.0 60 43.0 61 37.5 62 18.0 63 30.0 64 40.0 65 30.5 66 20.5 67 7.5 68 4.5 69 3.5 70 4.0 71 5.0 72 4.0 73 3.0 74 2.0 75 1.0 76 1.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.15 3 0.15 4 0.15 5 0.15 6 0.15 7 0.2 8 0.15 9 0.15 10-14 0.20500000000000002 15-19 0.22499999999999998 20-24 0.18 25-29 0.19 30-34 0.18 35-39 0.19 40-44 0.15 45-49 0.15 50-54 0.15 55-59 0.15 60-64 0.15 65-69 0.15 70-74 0.15 75-79 0.135 80-84 0.13999999999999999 85-89 0.15 90-94 0.15 95-99 0.15 100-104 0.15 105-109 0.15 110-114 0.15 115-119 0.15 120-124 0.15 125-129 0.15 130-134 0.15 135-139 0.15 140-144 0.15 145-149 0.15 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.4 #Duplication Level Percentage of deduplicated Percentage of total 1 87.73209549071618 66.14999999999999 2 6.001326259946949 9.049999999999999 3 2.8183023872679045 6.375 4 1.1936339522546418 3.5999999999999996 5 0.3978779840848806 1.5 6 0.5636604774535808 2.55 7 0.4641909814323607 2.45 8 0.1989389920424403 1.2 9 0.1326259946949602 0.8999999999999999 >10 0.49734748010610075 6.225 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 27 0.675 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 26 0.65 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 25 0.625 No Hit GTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACTGAATTTAAAATG 20 0.5 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 18 0.44999999999999996 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 16 0.4 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 16 0.4 No Hit CTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGT 15 0.375 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 14 0.35000000000000003 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 14 0.35000000000000003 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 14 0.35000000000000003 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 13 0.325 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 11 0.27499999999999997 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 10 0.25 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 10 0.25 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 9 0.22499999999999998 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 9 0.22499999999999998 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 9 0.22499999999999998 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 9 0.22499999999999998 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 8 0.2 No Hit CGTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGAC 8 0.2 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 8 0.2 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 8 0.2 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 7 0.17500000000000002 No Hit CTCGTTTACACGTGCGCCAATGCTTTTCAAAGGAGCTTATTATGCAATGA 7 0.17500000000000002 No Hit CAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTT 7 0.17500000000000002 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 7 0.17500000000000002 No Hit CAGTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGAC 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 7 0.17500000000000002 No Hit CTGTTCTATAGGATCGTACCGCTACATCCTTTACCAAAAAGGAGGCAAGA 7 0.17500000000000002 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 7 0.17500000000000002 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 7 0.17500000000000002 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 6 0.15 No Hit GTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGACT 6 0.15 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 6 0.15 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit AGCTCAGTTCGGTAGAGCAACTCGTTTACACGTGCGCCAATGCTTTTCAA 6 0.15 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 6 0.15 No Hit CTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAACCATTTATGCG 6 0.15 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 6 0.15 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 6 0.15 No Hit CACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTT 6 0.15 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 6 0.15 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 6 0.15 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 6 0.15 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6 0.15 No Hit TGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAAT 5 0.125 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit CTCAGTTCGGTAGAGCAACTCGTTTACACGTGCGCCAATGCTTTTCAAAG 5 0.125 No Hit CTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTT 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit CACGAACGTAATGCTCACAACTTCCCTCTAGATCTAGCTGCTCTTGAAGT 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit CTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.0625 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.2375 0.0 0.0 0.0 0.0 94-95 0.275 0.0 0.0 0.0 0.0 96-97 0.3375 0.0 0.0 0.0 0.0 98-99 0.4125 0.0 0.0 0.0 0.0 100-101 0.5125 0.0 0.0 0.0 0.0 102-103 0.7125 0.0 0.0 0.0 0.0 104-105 0.9125 0.0 0.0 0.0 0.0 106-107 1.075 0.0 0.0 0.0 0.0 108-109 1.4125 0.0 0.0 0.0 0.0 110-111 1.7125 0.0 0.0 0.0 0.0 112-113 2.0625 0.0 0.0 0.0 0.0 114-115 2.4749999999999996 0.0 0.0 0.0 0.0 116-117 2.9625 0.0 0.0 0.0 0.0 118-119 3.3625 0.0 0.0 0.0 0.0 120-121 3.8625 0.0 0.0 0.0 0.0 122-123 4.4875 0.0 0.0 0.0 0.0 124-125 5.1625 0.0 0.0 0.0 0.0 126-127 5.725 0.0 0.0 0.0 0.0 128-129 6.125 0.0 0.0 0.0 0.0 130-131 6.675000000000001 0.0 0.0 0.0 0.0 132-133 7.45 0.0 0.0 0.0 0.0 134-135 8.0375 0.0 0.0 0.0 0.0 136-137 8.9 0.0 0.0 0.0 0.0 138-139 9.712499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGAG 10 0.006830828 145.0 4 CTATATT 10 0.006830828 145.0 1 TATATTA 10 0.006830828 145.0 2 AGTAGCG 10 0.006830828 145.0 2 CGAGAGC 10 0.006830828 145.0 7 GTAGCGA 10 0.006830828 145.0 3 >>END_MODULE Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557020 spots for SRR8846520.sra Written 1557020 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra Read 1557014 spots for SRR8846520.sra Written 1557014 spots for SRR8846520.sra SRR ids: ['SRR8846520.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wyofedgc SRR8846520.sra spots: 31140286 blocks: [[1, 1557014], [1557015, 3114028], [3114029, 4671042], [4671043, 6228056], [6228057, 7785070], [7785071, 9342084], [9342085, 10899098], [10899099, 12456112], [12456113, 14013126], [14013127, 15570140], [15570141, 17127154], [17127155, 18684168], [18684169, 20241182], [20241183, 21798196], [21798197, 23355210], [23355211, 24912224], [24912225, 26469238], [26469239, 28026252], [28026253, 29583266], [29583267, 31140286]] SRR8846520 file size 10530720 SRR8846520 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846520 SRR8846520_1.fastq SRR8846520_2.fastq Input file: SRR8846520_1.fastq Paired file: SRR8846520_2.fastq trimmed: SRR8846520-trimmed-pair1.fastq, SRR8846520-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 05:37:00 2024 >> started Mon Dec 9 05:40:03 2024 >> done (183.199s) 31140286 read pairs processed; of these: 18336 ( 0.06%) short read pairs filtered out after trimming by size control 92798 ( 0.30%) empty read pairs filtered out after trimming by size control 31029152 (99.64%) read pairs available; of these: 13538456 (43.63%) trimmed read pairs available after processing 17490696 (56.37%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 13 0.00% 19 7 0.00% 20 6 0.00% 21 7 0.00% 22 6 0.00% 23 9 0.00% 24 7 0.00% 25 6 0.00% 26 6 0.00% 27 16 0.00% 28 10 0.00% 29 6 0.00% 30 14 0.00% 31 5 0.00% 32 11 0.00% 33 12 0.00% 34 13 0.00% 35 12 0.00% 36 12 0.00% 37 20 0.00% 38 20 0.00% 39 24 0.00% 40 21 0.00% 41 27 0.00% 42 30 0.00% 43 38 0.00% 44 43 0.00% 45 55 0.00% 46 37 0.00% 47 52 0.00% 48 82 0.00% 49 80 0.00% 50 90 0.00% 51 77 0.00% 52 112 0.00% 53 113 0.00% 54 122 0.00% 55 175 0.00% 56 149 0.00% 57 162 0.00% 58 184 0.00% 59 224 0.00% 60 227 0.00% 61 261 0.00% 62 316 0.00% 63 403 0.00% 64 421 0.00% 65 467 0.00% 66 515 0.00% 67 580 0.00% 68 649 0.00% 69 741 0.00% 70 865 0.00% 71 932 0.00% 72 1045 0.00% 73 1240 0.00% 74 1430 0.00% 75 1547 0.00% 76 1786 0.01% 77 1894 0.01% 78 2181 0.01% 79 2213 0.01% 80 2716 0.01% 81 3184 0.01% 82 3646 0.01% 83 4005 0.01% 84 4985 0.02% 85 6308 0.02% 86 6727 0.02% 87 7210 0.02% 88 7918 0.03% 89 8379 0.03% 90 9996 0.03% 91 10265 0.03% 92 11832 0.04% 93 13039 0.04% 94 15151 0.05% 95 15882 0.05% 96 17289 0.06% 97 18170 0.06% 98 20721 0.07% 99 22641 0.07% 100 24151 0.08% 101 26789 0.09% 102 29785 0.10% 103 31280 0.10% 104 35089 0.11% 105 39817 0.13% 106 43456 0.14% 107 45185 0.15% 108 46834 0.15% 109 55461 0.18% 110 55019 0.18% 111 60343 0.19% 112 60652 0.20% 113 59152 0.19% 114 62773 0.20% 115 67257 0.22% 116 73614 0.24% 117 80022 0.26% 118 79616 0.26% 119 87998 0.28% 120 90838 0.29% 121 93534 0.30% 122 96298 0.31% 123 98110 0.32% 124 105390 0.34% 125 120387 0.39% 126 115366 0.37% 127 123453 0.40% 128 130887 0.42% 129 141671 0.46% 130 135409 0.44% 131 151981 0.49% 132 140707 0.45% 133 146984 0.47% 134 153133 0.49% 135 154054 0.50% 136 168149 0.54% 137 168607 0.54% 138 185872 0.60% 139 192152 0.62% 140 199588 0.64% 141 227065 0.73% 142 216797 0.70% 143 244905 0.79% 144 267295 0.86% 145 318853 1.03% 146 336059 1.08% 147 423683 1.37% 148 584001 1.88% 149 1055308 3.40% 150 5655735 18.23% 151 17490696 56.37% 31029152 reads passed initial QC criterion=sequence-density sequence-density=3.04 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=33 prefix-density=2.96 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=1633.15 fanout-score-rank=1 prefix-density=9.70 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.71 sequence-density-rank=1 fanout-score=1.79 fanout-score-rank=34 prefix-density=3.05 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=165.07 fanout-score-rank=1 prefix-density=0.53 prefix-fanout=3.7 sequence=AAAGGAAAATGGGGATATGGCGAAATCGGTAGACGCTACGGACTTGATTGTATTGAGCCTTAGTATGGAAACCTGCTAAGTGTTAACTTCCAAATTCAGAGAAACCCTGGAATTAAAAAAGGGCAATCCTGAGCCAAATCCGTGTTTTGAGAAAACAAGGGGTTCTCGAACTAGAATCCAAAGGAAAAGGATAGGTGCAGAGACTCAATGGAAGCTGTTCTAACGAATCGAGTTAATTTATTTAGGTTGTTTTGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x CCAGCCTCACGC -y TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG -o SRR8846520 SRR8846520_1.fastq SRR8846520_2.fastq Input file: SRR8846520_1.fastq Paired file: SRR8846520_2.fastq trimmed: SRR8846520-trimmed-pair1.fastq, SRR8846520-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): CCAGCCTCACGC -- paired 3' end adapter sequence (-y): TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 05:54:48 2024 >> started Mon Dec 9 05:55:49 2024 >> done (60.410s) 10343051 read pairs processed; of these: 1320 ( 0.01%) short read pairs filtered out after trimming by size control 3608 ( 0.03%) empty read pairs filtered out after trimming by size control 10338123 (99.95%) read pairs available; of these: 1491 ( 0.01%) trimmed read pairs available after processing 10336632 (99.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 2 0.00% 20 3 0.00% 21 3 0.00% 22 1 0.00% 23 3 0.00% 24 1 0.00% 25 3 0.00% 26 1 0.00% 27 5 0.00% 28 2 0.00% 29 2 0.00% 30 1 0.00% 31 0 0.00% 32 2 0.00% 33 3 0.00% 34 0 0.00% 35 3 0.00% 36 7 0.00% 37 6 0.00% 38 5 0.00% 39 5 0.00% 40 7 0.00% 41 9 0.00% 42 9 0.00% 43 15 0.00% 44 19 0.00% 45 17 0.00% 46 14 0.00% 47 20 0.00% 48 28 0.00% 49 32 0.00% 50 33 0.00% 51 22 0.00% 52 43 0.00% 53 33 0.00% 54 40 0.00% 55 69 0.00% 56 56 0.00% 57 59 0.00% 58 55 0.00% 59 71 0.00% 60 82 0.00% 61 82 0.00% 62 103 0.00% 63 140 0.00% 64 130 0.00% 65 153 0.00% 66 179 0.00% 67 209 0.00% 68 214 0.00% 69 232 0.00% 70 283 0.00% 71 341 0.00% 72 344 0.00% 73 437 0.00% 74 485 0.00% 75 532 0.01% 76 552 0.01% 77 641 0.01% 78 715 0.01% 79 742 0.01% 80 883 0.01% 81 1069 0.01% 82 1219 0.01% 83 1282 0.01% 84 1621 0.02% 85 2153 0.02% 86 2214 0.02% 87 2452 0.02% 88 2663 0.03% 89 2739 0.03% 90 3410 0.03% 91 3361 0.03% 92 3950 0.04% 93 4424 0.04% 94 5103 0.05% 95 5328 0.05% 96 5718 0.06% 97 6108 0.06% 98 6887 0.07% 99 7471 0.07% 100 8064 0.08% 101 8954 0.09% 102 9825 0.10% 103 10391 0.10% 104 11759 0.11% 105 13355 0.13% 106 14455 0.14% 107 14939 0.14% 108 15589 0.15% 109 18533 0.18% 110 18351 0.18% 111 20069 0.19% 112 20197 0.20% 113 19810 0.19% 114 20894 0.20% 115 22508 0.22% 116 24605 0.24% 117 26615 0.26% 118 26493 0.26% 119 29339 0.28% 120 30242 0.29% 121 31153 0.30% 122 32029 0.31% 123 32685 0.32% 124 34982 0.34% 125 40090 0.39% 126 38274 0.37% 127 40927 0.40% 128 43704 0.42% 129 47424 0.46% 130 45457 0.44% 131 50842 0.49% 132 47289 0.46% 133 48928 0.47% 134 51119 0.49% 135 51071 0.49% 136 55988 0.54% 137 56186 0.54% 138 61850 0.60% 139 64033 0.62% 140 66313 0.64% 141 75615 0.73% 142 72504 0.70% 143 81455 0.79% 144 89244 0.86% 145 106178 1.03% 146 111638 1.08% 147 141147 1.37% 148 194667 1.88% 149 351725 3.40% 150 1885628 18.24% 151 5825625 56.35% criterion=sequence-density sequence-density=2.95 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=32 prefix-density=2.91 prefix-fanout=2.0 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=1450.78 fanout-score-rank=1 prefix-density=9.85 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.67 sequence-density-rank=1 fanout-score=1.77 fanout-score-rank=33 prefix-density=2.96 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=170.71 fanout-score-rank=1 prefix-density=0.55 prefix-fanout=3.6 sequence=AAAGGAAAATGGGGATATGGCGAAATCGGTAGACGCTACGGACTTGATTGTATTGAGCCTTAGTATGGAAACCTGCTAAGTGTTAACTTCCAAATTCAGAGAAACCCTGGAATTAAAAAAGGGCAATCCTGAGCCAAATCCGTGTTTTGAGAAAACAAGGGGTTCTCGAACTAGAATCCAAAGGAAAAGGATAGGTGCAGAGACTCAATGGAAGCTGTTCTAACGAATCGAGTTAATTTATTTAGGTTGTTTTGG SRR8846520 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 06:00:29 Started mapping on | Dec 09 06:00:30 Finished on | Dec 09 06:12:55 Mapping speed, Million of reads per hour | 149.92 Number of input reads | 31024224 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 18240247 Uniquely mapped reads % | 58.79% Average mapped length | 293.63 Number of splices: Total | 3594556 Number of splices: Annotated (sjdb) | 3217490 Number of splices: GT/AG | 3456854 Number of splices: GC/AG | 40282 Number of splices: AT/AC | 12150 Number of splices: Non-canonical | 85270 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 1.76 Insertion rate per base | 0.02% Insertion average length | 1.76 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11557324 % of reads mapped to multiple loci | 37.25% Number of reads mapped to too many loci | 3731 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.46% % of reads unmapped: other | 0.48% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1236356 1236356 1236356 N_multimapping 11557324 11557324 11557324 N_noFeature 4772852 17286219 5202439 N_ambiguous 1087981 38423 571941 UnstrandedReadsAssigned:12379414 PositiveStrandReadsAssigned:915605 NegativeStrandReadsAssigned:12465867 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR8846520 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846520-trimmed-pair1.fastq SRR8846520-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,024,224 reads, 17,592,340 reads pseudoaligned [quant] estimated average fragment length: 221.214 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,026 rounds 52973 SRR8846520.ke.tsv 35125 SRR8846520.se.tsv 88098 total ==> SRR8846520.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 716.358 0 0 PNS24247 1044 823.786 10.5065 0.765823 PNS24249 1928 1707.79 12.2082 0.429242 PNS24246 1044 823.786 10.5065 0.765823 PNS24248 1044 823.786 10.5065 0.765823 PNS24244 1471 1250.79 41.2724 1.98136 PNS24243 293 107.567 0 0 KQK14069 1603 1382.79 1546.39 67.1507 KQK14071 474 262.992 13.4911 3.08029 ==> SRR8846520.se.tsv <== BRADI_1g14170v3 1771 BRADI_1g53295v3 26 BRADI_1g59795v3 32 BRADI_1g07683v3 0 BRADI_1g00485v3 17 BRADI_1g20270v3 310 BRADI_1g74790v3 23 BRADI_1g09890v3 0 BRADI_1g77505v3 67 BRADI_1g48960v3 0 SRR8846520 completed mapping pipeline successfully