Starting /dee2/code/volunteer_pipeline.sh SRR8846525 current disk space = 2792277929984 free memory = 1543661888 SRR8846525 SRAfilesize ffd281885dc2be9c25f100b9bdace063 SRR8846525.sra SRR8846525.sra file validated SRR8846525 is paired end SRR8846525 is conventional basespace SRR8846525 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846525_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.27275 34.0 33.0 34.0 33.0 34.0 2 33.26 34.0 33.0 34.0 33.0 34.0 3 33.23625 34.0 33.0 34.0 32.0 34.0 4 33.187 34.0 33.0 34.0 32.0 34.0 5 33.12225 34.0 33.0 34.0 32.0 34.0 6 36.672 38.0 37.0 38.0 34.0 38.0 7 37.0575 38.0 38.0 38.0 36.0 38.0 8 37.049 38.0 38.0 38.0 36.0 38.0 9 37.167 38.0 38.0 38.0 36.0 38.0 10-14 37.15235 38.0 38.0 38.0 36.0 38.0 15-19 37.13565 38.0 38.0 38.0 36.0 38.0 20-24 37.226800000000004 38.0 38.0 38.0 36.4 38.0 25-29 37.12 38.0 38.0 38.0 36.2 38.0 30-34 37.106 38.0 38.0 38.0 36.0 38.0 35-39 37.04379999999999 38.0 38.0 38.0 36.0 38.0 40-44 37.0574 38.0 38.0 38.0 36.0 38.0 45-49 36.99255 38.0 38.0 38.0 35.8 38.0 50-54 36.8576 38.0 38.0 38.0 35.2 38.0 55-59 36.40740000000001 38.0 38.0 38.0 34.6 38.0 60-64 35.4848 38.0 38.0 38.0 32.6 38.0 65-69 36.268950000000004 38.0 38.0 38.0 32.6 38.0 70-74 36.660849999999996 38.0 38.0 38.0 34.4 38.0 75-79 36.545249999999996 38.0 38.0 38.0 34.0 38.0 80-84 36.39175 38.0 38.0 38.0 34.0 38.0 85-89 36.3041 38.0 38.0 38.0 34.0 38.0 90-94 36.2856 38.0 38.0 38.0 33.8 38.0 95-99 36.191399999999994 38.0 37.6 38.0 33.2 38.0 100-104 35.95925 38.0 37.0 38.0 32.4 38.0 105-109 35.8198 38.0 37.0 38.0 31.8 38.0 110-114 35.60625 38.0 37.0 38.0 30.2 38.0 115-119 35.5355 38.0 37.0 38.0 31.0 38.0 120-124 35.34965 38.0 36.0 38.0 29.6 38.0 125-129 34.8815 38.0 35.2 38.0 27.8 38.0 130-134 34.53475 38.0 35.2 38.0 25.6 38.0 135-139 33.85915 38.0 34.2 38.0 21.8 38.0 140-144 33.325599999999994 38.0 33.0 38.0 19.0 38.0 145-149 32.80185 38.0 33.0 38.0 15.0 38.0 150-151 28.58275 34.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 0.0 11 0.0 12 0.0 13 4.0 14 1.0 15 1.0 16 7.0 17 0.0 18 4.0 19 4.0 20 6.0 21 11.0 22 10.0 23 9.0 24 24.0 25 22.0 26 21.0 27 32.0 28 47.0 29 48.0 30 58.0 31 97.0 32 98.0 33 144.0 34 197.0 35 295.0 36 602.0 37 2255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.900000000000002 9.225 8.375 59.5 2 17.825 10.225 46.575 25.374999999999996 3 14.174999999999999 14.85 31.175000000000004 39.800000000000004 4 20.175 23.075000000000003 29.475 27.275 5 21.61080540270135 28.38919459729865 29.139569784892444 20.860430215107552 6 17.375 34.175 31.15 17.299999999999997 7 12.375 27.950000000000003 45.925 13.750000000000002 8 14.774999999999999 27.375 39.275 18.575 9 14.95 23.075000000000003 40.425 21.55 10-14 17.265 34.410000000000004 26.724999999999998 21.6 15-19 17.757102841136454 32.68807523009203 28.701480592236894 20.853341336534616 20-24 17.26 31.130000000000003 30.29 21.32 25-29 21.099999999999998 31.095 28.83 18.975 30-34 22.37 30.56 26.105 20.965 35-39 19.595000000000002 34.21 25.95 20.244999999999997 40-44 17.835 30.709999999999997 29.03 22.425 45-49 18.475 31.34 27.97 22.215 50-54 18.14 32.324999999999996 28.360000000000003 21.175 55-59 21.519179259109517 30.449284884014755 25.38535402031637 22.646181836559357 60-64 18.829991191253434 32.66490491735323 27.374475361417687 21.130628529975645 65-69 20.991194968553458 31.205031446540882 25.916981132075474 21.88679245283019 70-74 21.58 31.435000000000002 24.085 22.900000000000002 75-79 20.77 31.795 26.93 20.505000000000003 80-84 22.985 30.235 25.965 20.815 85-89 22.75 30.099999999999998 26.295 20.855 90-94 21.008151222683402 31.434715207281094 26.964044606691 20.593088963344503 95-99 21.055 30.755 26.740000000000002 21.45 100-104 18.815 31.435000000000002 26.685 23.064999999999998 105-109 20.79 28.225 28.215 22.770000000000003 110-114 19.395 30.54 28.310000000000002 21.755 115-119 18.07 30.695 28.275 22.96 120-124 17.244999999999997 32.255 25.64 24.86 125-129 20.588088213231984 32.80992148822323 23.923588538280743 22.678401760264038 130-134 22.562256225622562 32.223222322232225 25.012501250125013 20.2020202020202 135-139 23.026908072421726 30.354106231869558 24.44733420026008 22.171651495448636 140-144 22.376118805940298 31.53657682884144 26.401320066003297 19.68598429921496 145-149 20.87 31.095 25.624999999999996 22.41 150-151 20.349999999999998 31.7 24.5375 23.4125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 1.5 21 2.0 22 8.5 23 11.5 24 10.5 25 10.5 26 15.0 27 22.5 28 25.5 29 34.5 30 43.0 31 54.0 32 60.0 33 65.5 34 90.0 35 105.5 36 153.0 37 332.5 38 367.5 39 236.5 40 226.5 41 233.5 42 214.5 43 262.0 44 255.5 45 209.0 46 177.5 47 114.5 48 91.5 49 74.5 50 58.5 51 49.5 52 29.0 53 19.5 54 26.0 55 28.5 56 23.5 57 22.5 58 24.5 59 21.0 60 21.5 61 24.0 62 19.0 63 14.0 64 22.0 65 27.5 66 14.0 67 7.0 68 7.0 69 4.5 70 5.5 71 5.0 72 4.0 73 2.5 74 3.0 75 3.0 76 1.0 77 0.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.05 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.04 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.065 60-64 3.505 65-69 0.625 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.015 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.015 130-134 0.01 135-139 0.03 140-144 0.005 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.849999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 84.37744714173844 53.87499999999999 2 8.339859044635865 10.65 3 2.662490211433046 5.1 4 1.2529365700861395 3.2 5 0.7439310884886452 2.375 6 0.6656225528582616 2.55 7 0.3523884103367267 1.575 8 0.19577133907595928 1.0 9 0.19577133907595928 1.125 >10 1.1354737666405637 12.7 >50 0.03915426781519186 2.375 >100 0.03915426781519186 3.4750000000000005 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 139 3.4750000000000005 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 95 2.375 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 49 1.225 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 41 1.0250000000000001 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 39 0.975 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 34 0.8500000000000001 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 30 0.75 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 26 0.65 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 20 0.5 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 16 0.4 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 15 0.375 No Hit CTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTT 15 0.375 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 14 0.35000000000000003 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 13 0.325 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 13 0.325 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 13 0.325 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 13 0.325 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 13 0.325 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 12 0.3 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 12 0.3 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 12 0.3 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 11 0.27499999999999997 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 11 0.27499999999999997 No Hit CTTGGTTTCATACTCCGGGGTGTAGTAAGTCAATCTATAATCTTTAACAC 11 0.27499999999999997 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 11 0.27499999999999997 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 11 0.27499999999999997 No Hit CTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAA 11 0.27499999999999997 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 11 0.27499999999999997 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 10 0.25 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 10 0.25 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 9 0.22499999999999998 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 9 0.22499999999999998 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 9 0.22499999999999998 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 9 0.22499999999999998 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 9 0.22499999999999998 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 8 0.2 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 8 0.2 No Hit CTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACA 8 0.2 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 8 0.2 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 8 0.2 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 7 0.17500000000000002 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 7 0.17500000000000002 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 7 0.17500000000000002 No Hit GTCCAAACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGC 7 0.17500000000000002 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 7 0.17500000000000002 No Hit ATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTCTCTCTAA 7 0.17500000000000002 No Hit CTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAA 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 7 0.17500000000000002 No Hit CAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGAT 6 0.15 No Hit CTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCT 6 0.15 No Hit CTCAATCAAAGATCCAACTGATCCCCACGCCTGTATTGCAAATACGCAGT 6 0.15 No Hit CCCCTTCTAACTTACCTACTACTGTACCGGCGTGGATATGATCTCCCCCA 6 0.15 No Hit CCCCAGTTAAGTAGTCATGCATTACAATAGGAACACCTAATTCTCTCGCA 6 0.15 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 6 0.15 No Hit CCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGACT 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit CTCTACCGTAATTTTTTGCAGATAATCCCAATTTTGGCTTAATAGTACAT 6 0.15 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 6 0.15 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 6 0.15 No Hit CCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCG 6 0.15 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 5 0.125 No Hit CGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGT 5 0.125 No Hit CCGTAATTTTTTGCAGATAATCCCAATTTTGGCTTAATAGTACATCCCAA 5 0.125 No Hit CTCGCAAAAACAGCTCTCTTCATCATTTCTTCACATGTACCCGCAGTTGC 5 0.125 No Hit CTTCTAACTTACCTACTACTGTACCGGCGTGGATATGATCTCCCCCAGAC 5 0.125 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 5 0.125 No Hit CTCGGTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTT 5 0.125 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 5 0.125 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 5 0.125 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 5 0.125 No Hit CTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGAT 5 0.125 No Hit CCTGCTTCTTCGGGCGGAACCCCAGGTTGAGGAGATACTCGGAATGCTGC 5 0.125 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 5 0.125 No Hit TGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGT 5 0.125 No Hit GTCCTTTGTAACGATCAAGACTAGTAAGTCCATCAGTCCAAACAGTTGTC 5 0.125 No Hit GTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCC 5 0.125 No Hit CCCAATTTTGGCTTAATAGTACATCCCAATAAAGGACGACCATACTTGTT 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit TTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.225 0.0 0.0 0.0 0.0 94-95 0.3125 0.0 0.0 0.0 0.0 96-97 0.525 0.0 0.0 0.0 0.0 98-99 0.6499999999999999 0.0 0.0 0.0 0.0 100-101 0.8125 0.0 0.0 0.0 0.0 102-103 1.0875 0.0 0.0 0.0 0.0 104-105 1.3375 0.0 0.0 0.0 0.0 106-107 1.6625 0.0 0.0 0.0 0.0 108-109 1.9 0.0 0.0 0.0 0.0 110-111 2.3375 0.0 0.0 0.0 0.0 112-113 2.6625 0.0 0.0 0.0 0.0 114-115 2.95 0.0 0.0 0.0 0.0 116-117 3.3 0.0 0.0 0.0 0.0 118-119 3.7625 0.0 0.0 0.0 0.0 120-121 4.475 0.0 0.0 0.0 0.0 122-123 5.1 0.0 0.0 0.0 0.0 124-125 5.550000000000001 0.0 0.0 0.0 0.0 126-127 6.25 0.0 0.0 0.0 0.0 128-129 7.0 0.0 0.0 0.0 0.0 130-131 8.05 0.0 0.0 0.0 0.0 132-133 8.8375 0.0 0.0 0.0 0.0 134-135 9.65 0.0 0.0 0.0 0.0 136-137 10.375 0.0 0.0 0.0 0.0 138-139 11.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTCAA 10 0.0066283476 146.44302 5 CAATTCA 10 0.0066283476 146.44302 4 TCCAAAA 10 0.0068857023 144.61249 8 CTTTCTT 25 8.3690014E-4 87.86582 1 TCTTTTC 40 0.0054001138 54.916138 4 TTTCTTT 45 0.008605246 48.81435 2 CAAAAAT 45 6.670243E-4 32.136112 9 TTCAAAA 50 5.72553E-5 28.9225 7 TCAAAAA 55 1.1910206E-4 26.293182 8 TTCAAGA 70 6.1746134E-4 20.658928 7 TGTTAGC 50 5.72553E-5 20.24575 25-29 TTCTTAT 50 5.72553E-5 20.24575 15-19 CTTATAT 50 5.72553E-5 20.24575 20-24 ATATGTT 50 5.72553E-5 20.24575 20-24 GCGGGAA 55 1.0186496E-4 18.780844 55-59 CGGGAAC 55 1.0186496E-4 18.780844 55-59 TAGCGGG 55 1.0805744E-4 18.638205 50-54 TCCATTA 55 1.1681833E-4 18.451355 45-49 AAATTCT 55 1.1910206E-4 18.405226 15-19 TTATATG 55 1.1910206E-4 18.405226 20-24 >>END_MODULE SRR8846525 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846525_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.546 33.0 33.0 34.0 32.0 34.0 2 32.753 33.0 33.0 34.0 32.0 34.0 3 32.79425 33.0 33.0 34.0 32.0 34.0 4 32.73475 33.0 33.0 34.0 32.0 34.0 5 32.74025 33.0 33.0 34.0 32.0 34.0 6 36.96875 38.0 38.0 38.0 36.0 38.0 7 36.994 38.0 38.0 38.0 36.0 38.0 8 36.97475 38.0 38.0 38.0 36.0 38.0 9 36.929 38.0 38.0 38.0 36.0 38.0 10-14 36.90755 38.0 38.0 38.0 36.0 38.0 15-19 36.8881 38.0 38.0 38.0 36.0 38.0 20-24 36.90235 38.0 38.0 38.0 36.0 38.0 25-29 36.81365 38.0 38.0 38.0 35.8 38.0 30-34 36.83489999999999 38.0 38.0 38.0 36.0 38.0 35-39 36.789249999999996 38.0 38.0 38.0 35.6 38.0 40-44 36.7678 38.0 38.0 38.0 35.4 38.0 45-49 36.7156 38.0 38.0 38.0 35.2 38.0 50-54 36.6255 38.0 38.0 38.0 35.0 38.0 55-59 36.536649999999995 38.0 38.0 38.0 34.6 38.0 60-64 36.528200000000005 38.0 38.0 38.0 34.4 38.0 65-69 36.46835 38.0 38.0 38.0 34.2 38.0 70-74 36.5001 38.0 38.0 38.0 34.0 38.0 75-79 36.45005 38.0 38.0 38.0 34.2 38.0 80-84 36.3393 38.0 38.0 38.0 34.0 38.0 85-89 36.2001 38.0 38.0 38.0 33.8 38.0 90-94 36.098349999999996 38.0 38.0 38.0 33.4 38.0 95-99 35.952149999999996 38.0 37.8 38.0 32.8 38.0 100-104 35.868449999999996 38.0 37.4 38.0 32.6 38.0 105-109 35.73395 38.0 37.0 38.0 31.4 38.0 110-114 35.52705 38.0 37.0 38.0 30.2 38.0 115-119 35.53144999999999 38.0 37.0 38.0 31.0 38.0 120-124 35.23855 38.0 36.6 38.0 29.2 38.0 125-129 34.8619 38.0 36.0 38.0 27.8 38.0 130-134 34.5303 38.0 35.0 38.0 26.2 38.0 135-139 34.140750000000004 38.0 35.0 38.0 23.4 38.0 140-144 33.471849999999996 38.0 34.6 38.0 18.8 38.0 145-149 32.2259 38.0 33.6 38.0 8.8 38.0 150-151 28.239375 35.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 0.0 4 0.0 5 1.0 6 0.0 7 1.0 8 0.0 9 0.0 10 3.0 11 1.0 12 2.0 13 3.0 14 5.0 15 0.0 16 3.0 17 6.0 18 8.0 19 4.0 20 15.0 21 8.0 22 11.0 23 14.0 24 10.0 25 20.0 26 26.0 27 30.0 28 45.0 29 41.0 30 48.0 31 90.0 32 78.0 33 140.0 34 162.0 35 253.0 36 570.0 37 2384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.34839357429719 20.33132530120482 16.54116465863454 44.77911646586345 2 19.43817406571357 21.720591923752195 44.971156257837976 13.870077752696265 3 14.998745924253825 28.342111863556557 37.873087534487084 18.78605467770253 4 19.834503510531594 30.391173520561686 29.81444332998997 19.95987963891675 5 21.63111668757842 34.80552070263488 30.112923462986195 13.450439146800502 6 18.67469879518072 36.119477911646584 30.220883534136544 14.984939759036145 7 15.82119537920643 20.341536916122553 47.08689100954294 16.75037669512808 8 18.04718875502008 23.920682730923694 36.49598393574297 21.536144578313255 9 20.20582329317269 19.201807228915662 40.18574297188755 20.406626506024097 10-14 21.55020846938263 27.673682624202538 31.46129502185161 19.314813884563222 15-19 21.988345222545966 26.775846478448713 32.593187983522554 18.64262031548277 20-24 22.08998694385859 26.609420508185195 32.650396705835085 18.65019584212112 25-29 22.60284293535587 25.953086543774173 32.56316238886935 18.880908132000602 30-34 24.20148654077943 25.903977501004423 31.317798312575327 18.57673764564082 35-39 22.998493219487695 26.464088397790054 30.803616273229533 19.733802109492718 40-44 21.23676153189781 27.75184460171661 31.380816142147268 19.630577724238318 45-49 21.811794228356337 28.26097867001255 30.840652446675033 19.086574654956085 50-54 21.627183296526802 28.202168239309376 30.209797229471995 19.96085123469183 55-59 20.42363097927019 28.896250564674 29.779651658886714 20.900466797169102 60-64 20.800923370301604 27.369900135494557 31.5351031264114 20.294073367792443 65-69 21.303497064873813 27.003160905122677 31.297978024183433 20.39536400582008 70-74 21.60345173590207 27.23259080874975 31.532209512341964 19.63174794300622 75-79 22.18604418173621 26.163402294244353 31.97916144868006 19.671392075339377 80-84 23.014083095273893 25.91590237057084 32.49135468350624 18.578659850649025 85-89 22.94374466803834 27.75631053344708 29.562904601796557 19.73704019671802 90-94 22.495482834772133 27.23348725155591 30.15458743224252 20.11644248142943 95-99 21.78998092560988 27.984138138741088 30.634474450356393 19.59140648529264 100-104 22.834052805943177 27.682963557875716 30.303182411404478 19.17980122477663 105-109 23.716307784972145 26.311298499222 30.73834261908347 19.234051096722382 110-114 23.124027505897708 26.13060282086031 30.341815991567533 20.403553681674445 115-119 21.90323228267416 27.48444087532624 30.74683798434049 19.865488857659106 120-124 22.02258469259724 28.095357590966124 29.27478042659975 20.60727728983689 125-129 24.062186559679034 27.437311935807422 29.368104312938815 19.132397191574725 130-134 23.042191340992325 28.53559424070637 29.473737019013697 18.948477399287615 135-139 23.880971497390608 28.417302288237657 29.18506623845845 18.51665997591329 140-144 24.49450604585821 28.663890421955745 28.84953088154132 17.992072650644726 145-149 24.257177273639833 27.58482232483437 28.924914675767916 19.233085725757878 150-151 25.297432686286786 27.213525360050095 29.643080776455854 17.845961177207265 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 3.5 2 0.5 3 1.0 4 1.0 5 1.5 6 1.0 7 0.5 8 0.5 9 0.5 10 0.5 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 1.0 17 1.5 18 0.5 19 1.5 20 4.0 21 4.0 22 3.5 23 7.0 24 12.0 25 17.0 26 18.0 27 26.5 28 33.5 29 41.5 30 62.0 31 63.5 32 65.0 33 84.0 34 126.5 35 172.5 36 168.5 37 202.0 38 253.5 39 231.0 40 231.0 41 262.5 42 249.5 43 236.0 44 231.5 45 204.0 46 175.0 47 134.0 48 102.5 49 78.0 50 62.5 51 59.5 52 40.5 53 24.5 54 17.0 55 18.0 56 18.0 57 15.0 58 15.0 59 18.5 60 21.5 61 17.0 62 16.5 63 22.0 64 21.0 65 22.0 66 17.5 67 8.5 68 9.0 69 10.0 70 7.0 71 3.0 72 3.0 73 5.0 74 5.0 75 2.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.325 3 0.325 4 0.3 5 0.375 6 0.4 7 0.44999999999999996 8 0.4 9 0.4 10-14 0.46499999999999997 15-19 0.47000000000000003 20-24 0.43 25-29 0.455 30-34 0.44 35-39 0.44999999999999996 40-44 0.385 45-49 0.375 50-54 0.38 55-59 0.385 60-64 0.365 65-69 0.345 70-74 0.33999999999999997 75-79 0.185 80-84 0.23500000000000001 85-89 0.365 90-94 0.38 95-99 0.38999999999999996 100-104 0.38999999999999996 105-109 0.385 110-114 0.385 115-119 0.38 120-124 0.375 125-129 0.3 130-134 0.335 135-139 0.36 140-144 0.345 145-149 0.38 150-151 0.1875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.7 #Duplication Level Percentage of deduplicated Percentage of total 1 84.52732644017725 57.225 2 7.828655834564254 10.6 3 2.6957163958641064 5.475 4 1.4401772525849335 3.9 5 0.5908419497784343 2.0 6 0.7385524372230428 3.0 7 0.48005908419497784 2.275 8 0.3692762186115214 2.0 9 0.3692762186115214 2.25 >10 0.9231905465288036 10.0 >50 0.03692762186115214 1.275 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 51 1.275 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 34 0.8500000000000001 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 29 0.7250000000000001 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 27 0.675 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 21 0.525 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 20 0.5 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 17 0.42500000000000004 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 17 0.42500000000000004 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 17 0.42500000000000004 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 16 0.4 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 15 0.375 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 15 0.375 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 14 0.35000000000000003 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 14 0.35000000000000003 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 14 0.35000000000000003 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 14 0.35000000000000003 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 14 0.35000000000000003 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 13 0.325 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 12 0.3 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 12 0.3 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12 0.3 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 11 0.27499999999999997 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 10 0.25 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 10 0.25 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 10 0.25 No Hit TTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATAC 9 0.22499999999999998 No Hit CTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCAC 9 0.22499999999999998 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 9 0.22499999999999998 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 9 0.22499999999999998 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 9 0.22499999999999998 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 9 0.22499999999999998 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 9 0.22499999999999998 No Hit CCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATA 9 0.22499999999999998 No Hit CTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACT 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 8 0.2 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 8 0.2 No Hit CTTTTTCACTCAGGACTGGGTATCCATGCCAGGTGTTATACCAGTAGCTT 8 0.2 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 8 0.2 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 8 0.2 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 8 0.2 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 8 0.2 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 7 0.17500000000000002 No Hit CAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTT 7 0.17500000000000002 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 7 0.17500000000000002 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit CTTGGTATGGAAGTAATGCACGAACGTAATGCTCACAACTTCCCTCTAGA 7 0.17500000000000002 No Hit CTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAACCATTTATGCG 7 0.17500000000000002 No Hit CTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGT 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit TATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATG 7 0.17500000000000002 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 6 0.15 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 6 0.15 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 6 0.15 No Hit CTGAATTTAAAATGATATACTTAAGGCATCCTTAAGTTTTTTTTCTATTC 6 0.15 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 6 0.15 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 6 0.15 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit ATCTTGGTATGGAAGTAATGCACGAACGTAATGCTCACAACTTCCCTCTA 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit GATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAA 6 0.15 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 6 0.15 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 6 0.15 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 6 0.15 No Hit CAAATCCTTGGTTTAATAACGAACGGTGTTAACTTACCATAACAACAACT 6 0.15 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 6 0.15 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 6 0.15 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit CTATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCA 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCAG 5 0.125 No Hit TATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAG 5 0.125 No Hit GTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAA 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit CGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATA 5 0.125 No Hit TTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGT 5 0.125 No Hit CTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAAC 5 0.125 No Hit ATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTGGAGGATCTACGAATTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.225 0.0 0.0 0.0 0.0 94-95 0.3125 0.0 0.0 0.0 0.0 96-97 0.525 0.0 0.0 0.0 0.0 98-99 0.6499999999999999 0.0 0.0 0.0 0.0 100-101 0.8125 0.0 0.0 0.0 0.0 102-103 1.1 0.0 0.0 0.0 0.0 104-105 1.3375 0.0 0.0 0.0 0.0 106-107 1.6625 0.0 0.0 0.0 0.0 108-109 1.9 0.0 0.0 0.0 0.0 110-111 2.3125 0.0 0.0 0.0 0.0 112-113 2.6375 0.0 0.0 0.0 0.0 114-115 2.9375 0.0 0.0 0.0 0.0 116-117 3.2625 0.0 0.0 0.0 0.0 118-119 3.7125000000000004 0.0 0.0 0.0 0.0 120-121 4.4 0.0 0.0 0.0 0.0 122-123 5.05 0.0 0.0 0.0 0.0 124-125 5.5 0.0 0.0 0.0 0.0 126-127 6.15 0.0 0.0 0.0 0.0 128-129 6.875 0.0 0.0 0.0 0.0 130-131 7.887499999999999 0.0 0.0 0.0 0.0 132-133 8.6375 0.0 0.0 0.0 0.0 134-135 9.475 0.0 0.0 0.0 0.0 136-137 10.2 0.0 0.0 0.0 0.0 138-139 11.100000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTATA 10 0.006830828 145.0 1 CCTTGCA 35 0.0035366106 20.714287 35-39 TGGGGTC 35 0.0035366106 20.714287 105-109 TCGCTTC 35 0.0035366106 20.714287 110-114 TACAAGC 35 0.0035366106 20.714287 95-99 GACGCGA 35 0.0035366106 20.714287 85-89 AAGATCT 35 0.0035366106 20.714287 55-59 CTTACCA 35 0.0035366106 20.714287 60-64 CTAGTTA 35 0.0035366106 20.714287 6 GCTTGGG 35 0.0035366106 20.714287 25-29 TAATATG 35 0.0035366106 20.714287 15-19 CTGCAAT 35 0.0035366106 20.714287 70-74 GCCTGTG 35 0.0035366106 20.714287 100-104 ATTTTAG 35 0.0035366106 20.714287 75-79 TGTGTGC 35 0.0035366106 20.714287 20-24 AACTGGA 40 0.0076550315 18.125 120-124 TCTGCAA 40 0.0076550315 18.125 115-119 GAAAGTA 40 0.0076550315 18.125 90-94 GGAGTCC 40 0.0076550315 18.125 30-34 >>END_MODULE Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578342 spots for SRR8846525.sra Written 1578342 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra Read 1578337 spots for SRR8846525.sra Written 1578337 spots for SRR8846525.sra SRR ids: ['SRR8846525.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_i_q7ehc3 SRR8846525.sra spots: 31566745 blocks: [[1, 1578337], [1578338, 3156674], [3156675, 4735011], [4735012, 6313348], [6313349, 7891685], [7891686, 9470022], [9470023, 11048359], [11048360, 12626696], [12626697, 14205033], [14205034, 15783370], [15783371, 17361707], [17361708, 18940044], [18940045, 20518381], [20518382, 22096718], [22096719, 23675055], [23675056, 25253392], [25253393, 26831729], [26831730, 28410066], [28410067, 29988403], [29988404, 31566745]] SRR8846525 file size 10675233 SRR8846525 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846525 SRR8846525_1.fastq SRR8846525_2.fastq Input file: SRR8846525_1.fastq Paired file: SRR8846525_2.fastq trimmed: SRR8846525-trimmed-pair1.fastq, SRR8846525-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Mar 17 02:00:26 2025 >> started Mon Mar 17 02:01:04 2025 >> done (38.341s) 31566745 read pairs processed; of these: 14628 ( 0.05%) short read pairs filtered out after trimming by size control 95207 ( 0.30%) empty read pairs filtered out after trimming by size control 31456910 (99.65%) read pairs available; of these: 14209854 (45.17%) trimmed read pairs available after processing 17247056 (54.83%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 4 0.00% 20 7 0.00% 21 12 0.00% 22 12 0.00% 23 6 0.00% 24 7 0.00% 25 9 0.00% 26 6 0.00% 27 41 0.00% 28 15 0.00% 29 5 0.00% 30 5 0.00% 31 5 0.00% 32 8 0.00% 33 7 0.00% 34 11 0.00% 35 8 0.00% 36 10 0.00% 37 15 0.00% 38 12 0.00% 39 9 0.00% 40 11 0.00% 41 24 0.00% 42 24 0.00% 43 23 0.00% 44 26 0.00% 45 26 0.00% 46 33 0.00% 47 45 0.00% 48 57 0.00% 49 57 0.00% 50 69 0.00% 51 60 0.00% 52 80 0.00% 53 97 0.00% 54 139 0.00% 55 118 0.00% 56 150 0.00% 57 142 0.00% 58 179 0.00% 59 176 0.00% 60 223 0.00% 61 275 0.00% 62 318 0.00% 63 345 0.00% 64 383 0.00% 65 491 0.00% 66 527 0.00% 67 546 0.00% 68 689 0.00% 69 774 0.00% 70 860 0.00% 71 947 0.00% 72 1127 0.00% 73 1318 0.00% 74 1618 0.01% 75 1989 0.01% 76 2199 0.01% 77 2445 0.01% 78 2679 0.01% 79 2743 0.01% 80 3260 0.01% 81 3887 0.01% 82 4463 0.01% 83 5137 0.02% 84 5562 0.02% 85 7515 0.02% 86 8075 0.03% 87 8888 0.03% 88 9500 0.03% 89 10079 0.03% 90 12364 0.04% 91 12537 0.04% 92 14840 0.05% 93 16492 0.05% 94 19735 0.06% 95 20851 0.07% 96 20868 0.07% 97 21327 0.07% 98 25860 0.08% 99 27447 0.09% 100 29768 0.09% 101 32893 0.10% 102 36900 0.12% 103 36181 0.12% 104 42147 0.13% 105 48768 0.16% 106 51737 0.16% 107 51526 0.16% 108 54921 0.17% 109 67280 0.21% 110 60611 0.19% 111 72241 0.23% 112 69813 0.22% 113 66309 0.21% 114 70272 0.22% 115 72354 0.23% 116 79877 0.25% 117 84774 0.27% 118 84012 0.27% 119 95215 0.30% 120 97158 0.31% 121 103690 0.33% 122 103454 0.33% 123 103318 0.33% 124 111271 0.35% 125 131071 0.42% 126 121349 0.39% 127 131292 0.42% 128 142039 0.45% 129 164926 0.52% 130 152098 0.48% 131 176119 0.56% 132 159390 0.51% 133 152372 0.48% 134 168759 0.54% 135 161629 0.51% 136 179690 0.57% 137 177851 0.57% 138 202637 0.64% 139 200631 0.64% 140 212622 0.68% 141 241012 0.77% 142 225393 0.72% 143 261796 0.83% 144 282390 0.90% 145 344159 1.09% 146 348742 1.11% 147 433920 1.38% 148 599525 1.91% 149 1092948 3.47% 150 5736065 18.23% 151 17247056 54.83% 31456910 reads passed initial QC criterion=sequence-density sequence-density=1.99 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=34 prefix-density=1.93 prefix-fanout=1.9 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=1547.78 fanout-score-rank=1 prefix-density=12.90 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAA criterion=sequence-density sequence-density=1.13 sequence-density-rank=1 fanout-score=1.71 fanout-score-rank=35 prefix-density=1.93 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.04 sequence-density-rank=26 fanout-score=30.44 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=7.4 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG SRR8846525 testing PE reads STAR mapping to Ensembl genome Started job on | Mar 17 02:01:37 Started mapping on | Mar 17 02:01:37 Finished on | Mar 17 02:04:37 Mapping speed, Million of reads per hour | 629.14 Number of input reads | 31456910 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 18035678 Uniquely mapped reads % | 57.33% Average mapped length | 293.66 Number of splices: Total | 2703939 Number of splices: Annotated (sjdb) | 2367497 Number of splices: GT/AG | 2535975 Number of splices: GC/AG | 28991 Number of splices: AT/AC | 20786 Number of splices: Non-canonical | 118187 Mismatch rate per base, % | 0.21% Deletion rate per base | 0.01% Deletion average length | 1.68 Insertion rate per base | 0.02% Insertion average length | 1.90 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12228028 % of reads mapped to multiple loci | 38.87% Number of reads mapped to too many loci | 6194 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.96% % of reads unmapped: other | 0.82% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1198900 1198900 1198900 N_multimapping 12228028 12228028 12228028 N_noFeature 4772620 17193557 5070467 N_ambiguous 1147124 20980 621065 UnstrandedReadsAssigned:12115934 PositiveStrandReadsAssigned:821141 NegativeStrandReadsAssigned:12344146 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=146 echo kmer=141 SRR8846525 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846525-trimmed-pair1.fastq SRR8846525-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,456,910 reads, 20,114,673 reads pseudoaligned [quant] estimated average fragment length: 215.943 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 987 rounds 52973 SRR8846525.ke.tsv 35125 SRR8846525.se.tsv 88098 total ==> SRR8846525.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 722.36 0 0 PNS24247 1044 829.057 5.65464 0.356585 PNS24249 1928 1713.06 20.9433 0.639168 PNS24246 1044 829.057 5.65464 0.356585 PNS24248 1044 829.057 5.65464 0.356585 PNS24244 1471 1256.06 19.0928 0.7947 PNS24243 293 108.865 0 0 KQK14069 1603 1388.06 380.657 14.3374 KQK14071 474 266.545 23.2764 4.56549 ==> SRR8846525.se.tsv <== BRADI_1g14170v3 824 BRADI_1g53295v3 5 BRADI_1g59795v3 67 BRADI_1g07683v3 0 BRADI_1g00485v3 6 BRADI_1g20270v3 323 BRADI_1g74790v3 12 BRADI_1g09890v3 1 BRADI_1g77505v3 36 BRADI_1g48960v3 0 SRR8846525 completed mapping pipeline successfully