Starting /dee2/code/volunteer_pipeline.sh SRR8846553 current disk space = 1527836856320 free memory = 1528508224 SRR8846553 SRAfilesize f6ddd6fca7e0e0de8d4a2c227f54c6db SRR8846553.sra SRR8846553.sra file validated SRR8846553 is paired end SRR8846553 is conventional basespace SRR8846553 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846553_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.241 34.0 33.0 34.0 32.0 34.0 2 33.2285 34.0 33.0 34.0 33.0 34.0 3 33.2565 34.0 33.0 34.0 32.0 34.0 4 33.21 34.0 33.0 34.0 32.0 34.0 5 33.20775 34.0 33.0 34.0 32.0 34.0 6 36.697 38.0 37.0 38.0 34.0 38.0 7 37.0515 38.0 38.0 38.0 36.0 38.0 8 37.08125 38.0 38.0 38.0 36.0 38.0 9 37.21725 38.0 38.0 38.0 36.0 38.0 10-14 37.168549999999996 38.0 38.0 38.0 36.2 38.0 15-19 37.22425 38.0 38.0 38.0 36.8 38.0 20-24 37.28625 38.0 38.0 38.0 37.0 38.0 25-29 37.1879 38.0 38.0 38.0 36.8 38.0 30-34 37.1543 38.0 38.0 38.0 36.0 38.0 35-39 37.085 38.0 38.0 38.0 36.0 38.0 40-44 37.107299999999995 38.0 38.0 38.0 36.0 38.0 45-49 37.108999999999995 38.0 38.0 38.0 36.0 38.0 50-54 36.969849999999994 38.0 38.0 38.0 36.0 38.0 55-59 36.551300000000005 38.0 38.0 38.0 35.0 38.0 60-64 35.56985 38.0 38.0 38.0 33.0 38.0 65-69 36.3813 38.0 38.0 38.0 33.6 38.0 70-74 36.78410000000001 38.0 38.0 38.0 35.0 38.0 75-79 36.7646 38.0 38.0 38.0 35.0 38.0 80-84 36.55785 38.0 38.0 38.0 34.0 38.0 85-89 36.40555 38.0 38.0 38.0 34.0 38.0 90-94 36.4255 38.0 38.0 38.0 34.0 38.0 95-99 36.237049999999996 38.0 38.0 38.0 33.6 38.0 100-104 35.9947 38.0 37.2 38.0 33.0 38.0 105-109 35.93975 38.0 37.0 38.0 32.8 38.0 110-114 35.7192 38.0 37.0 38.0 31.0 38.0 115-119 35.648399999999995 38.0 36.8 38.0 31.0 38.0 120-124 35.34080000000001 38.0 36.0 38.0 30.0 38.0 125-129 34.91175 38.0 35.4 38.0 27.4 38.0 130-134 34.688399999999994 38.0 35.2 38.0 26.6 38.0 135-139 34.077749999999995 38.0 34.6 38.0 22.8 38.0 140-144 33.551300000000005 38.0 33.0 38.0 21.8 38.0 145-149 32.959050000000005 38.0 33.0 38.0 14.6 38.0 150-151 28.717375 35.0 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 0.0 10 1.0 11 0.0 12 0.0 13 3.0 14 0.0 15 1.0 16 2.0 17 4.0 18 3.0 19 6.0 20 1.0 21 5.0 22 7.0 23 13.0 24 16.0 25 25.0 26 19.0 27 36.0 28 31.0 29 43.0 30 60.0 31 89.0 32 82.0 33 130.0 34 212.0 35 348.0 36 605.0 37 2254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.325000000000003 10.0 9.975000000000001 56.699999999999996 2 18.325 12.925 42.4 26.35 3 17.375 17.0 29.725 35.9 4 21.75 24.8 26.85 26.6 5 21.660830415207606 29.864932466233117 30.14007003501751 18.33416708354177 6 18.425 33.875 28.925 18.775 7 13.25 27.224999999999998 45.1 14.424999999999999 8 16.275000000000002 26.200000000000003 38.824999999999996 18.7 9 16.45 22.15 40.425 20.974999999999998 10-14 17.91 33.615 26.275 22.2 15-19 19.083816763352672 31.901380276055214 28.155631126225245 20.859171834366876 20-24 18.595 30.39 28.999999999999996 22.015 25-29 21.025 30.819999999999997 27.689999999999998 20.465 30-34 22.375 30.830000000000002 25.27 21.525 35-39 20.41 33.855000000000004 24.6 21.135 40-44 18.404999999999998 30.869999999999997 27.905 22.82 45-49 19.54 30.955 26.965 22.54 50-54 18.85 31.290000000000003 28.395 21.465 55-59 21.20921305182342 29.957571471865847 25.361147590665723 23.472067885645014 60-64 19.448912874267034 31.39951222043485 27.336412225623995 21.81516267967412 65-69 21.23315228324281 31.663649165157914 25.271575135787565 21.831623415811706 70-74 22.335 30.615 23.855 23.195 75-79 21.490000000000002 31.185000000000002 26.479999999999997 20.845 80-84 22.99 30.270000000000003 26.02 20.72 85-89 23.044999999999998 30.225 25.605 21.125 90-94 21.886094304715236 30.291514575728783 27.071353567678386 20.751037551877594 95-99 21.3 30.775000000000002 26.27 21.654999999999998 100-104 19.305 31.15 25.974999999999998 23.57 105-109 20.745 28.110000000000003 27.29 23.855 110-114 19.595000000000002 29.955 27.439999999999998 23.01 115-119 19.265 30.464999999999996 27.544999999999998 22.725 120-124 17.645 31.81 25.755 24.79 125-129 20.49204920492049 33.1983198319832 23.612361236123615 22.697269726972696 130-134 21.986099304965247 32.011600580029004 25.231261563078156 20.771038551927596 135-139 22.584516903380678 30.526105221044208 24.114822964592918 22.774554910982197 140-144 21.92609630481524 31.001550077503875 26.561328066403323 20.511025551277566 145-149 20.09 31.455 26.05 22.405 150-151 21.087500000000002 30.7375 24.15 24.025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.5 22 5.0 23 8.0 24 12.5 25 17.5 26 17.5 27 20.0 28 25.5 29 32.5 30 42.0 31 53.5 32 60.5 33 66.0 34 80.5 35 93.5 36 137.5 37 296.0 38 342.0 39 234.5 40 216.0 41 229.5 42 213.0 43 240.0 44 246.0 45 201.0 46 163.0 47 120.0 48 94.5 49 68.5 50 55.0 51 48.5 52 30.0 53 29.5 54 40.0 55 39.0 56 30.5 57 26.0 58 34.0 59 42.5 60 39.0 61 29.5 62 25.0 63 23.0 64 41.0 65 49.5 66 23.5 67 9.0 68 9.5 69 9.0 70 6.5 71 5.0 72 4.0 73 4.5 74 4.5 75 2.5 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.05 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.02 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.01 60-64 3.6450000000000005 65-69 0.58 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.01 130-134 0.005 135-139 0.02 140-144 0.005 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.0 #Duplication Level Percentage of deduplicated Percentage of total 1 86.8840579710145 59.95 2 6.956521739130435 9.6 3 2.1739130434782608 4.5 4 1.2681159420289856 3.5000000000000004 5 0.7246376811594203 2.5 6 0.5434782608695652 2.25 7 0.2898550724637681 1.4000000000000001 8 0.21739130434782608 1.2 9 0.14492753623188406 0.8999999999999999 >10 0.7246376811594203 9.125 >50 0.036231884057971016 2.375 >100 0.036231884057971016 2.7 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 108 2.7 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 95 2.375 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 44 1.0999999999999999 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 41 1.0250000000000001 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 37 0.9249999999999999 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 29 0.7250000000000001 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 23 0.575 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 22 0.5499999999999999 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 17 0.42500000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 15 0.375 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 14 0.35000000000000003 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 13 0.325 No Hit GTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCC 13 0.325 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 12 0.3 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 12 0.3 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 11 0.27499999999999997 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 11 0.27499999999999997 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 11 0.27499999999999997 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 10 0.25 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 10 0.25 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 10 0.25 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 10 0.25 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 9 0.22499999999999998 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 9 0.22499999999999998 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 9 0.22499999999999998 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 8 0.2 No Hit CCAGAAACAGGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGAT 8 0.2 No Hit CTTTTTTTGATTGTCTGTCAATCAATATTCTAATGGCAATGCAATTTCAT 7 0.17500000000000002 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 7 0.17500000000000002 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 7 0.17500000000000002 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 7 0.17500000000000002 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 6 0.15 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 6 0.15 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 6 0.15 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit GGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGAC 6 0.15 No Hit CTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACTGTCT 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 6 0.15 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 6 0.15 No Hit CCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCA 6 0.15 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 6 0.15 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 6 0.15 No Hit CTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCATAC 6 0.15 No Hit CTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTT 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit CTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 5 0.125 No Hit GTTCTTTGGAGTAGGCTATGAGACCCAAGCGGGCCAGGAATGCAGCGGCC 5 0.125 No Hit CCAAGGATGTCCTAAAGTTCCTCCACCAAATTGTAATACAGAATCATCCC 5 0.125 No Hit CGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCC 5 0.125 No Hit CGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGC 5 0.125 No Hit CTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAA 5 0.125 No Hit CCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCTACACC 5 0.125 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 5 0.125 No Hit CATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATATCTACT 5 0.125 No Hit GCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCAT 5 0.125 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit GTGGTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCG 5 0.125 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 5 0.125 No Hit CCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.32499999999999996 0.0 0.0 0.0 0.0 90-91 0.3625 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.525 0.0 0.0 0.0 0.0 96-97 0.6 0.0 0.0 0.0 0.0 98-99 0.675 0.0 0.0 0.0 0.0 100-101 0.75 0.0 0.0 0.0 0.0 102-103 0.85 0.0 0.0 0.0 0.0 104-105 0.975 0.0 0.0 0.0 0.0 106-107 1.1749999999999998 0.0 0.0 0.0 0.0 108-109 1.35 0.0 0.0 0.0 0.0 110-111 1.5 0.0 0.0 0.0 0.0 112-113 1.8375 0.0 0.0 0.0 0.0 114-115 2.1624999999999996 0.0 0.0 0.0 0.0 116-117 2.5 0.0 0.0 0.0 0.0 118-119 2.9375 0.0 0.0 0.0 0.0 120-121 3.4625000000000004 0.0 0.0 0.0 0.0 122-123 3.9499999999999997 0.0 0.0 0.0 0.0 124-125 4.5125 0.0 0.0 0.0 0.0 126-127 5.075 0.0 0.0 0.0 0.0 128-129 5.7625 0.0 0.0 0.0 0.0 130-131 6.5375 0.0 0.0 0.0 0.0 132-133 7.1625 0.0 0.0 0.0 0.0 134-135 7.9875 0.0 0.0 0.0 0.0 136-137 8.6875 0.0 0.0 0.0 0.0 138-139 9.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTGAT 10 0.006882143 144.6375 4 AGTAGTG 10 0.006882143 144.6375 2 TGATCTC 10 0.006882143 144.6375 7 CCCCGTG 10 0.006882143 144.6375 145 GTGATCT 10 0.006882143 144.6375 6 GTAGTGA 10 0.006882143 144.6375 3 CTTTCTT 20 1.9029103E-6 144.6375 1 TTTCTTT 40 5.9592232E-5 72.31875 2 TTCTTTT 40 5.9592232E-5 72.31875 3 TCTTTTC 40 5.9592232E-5 72.31875 4 TTTTCTT 45 1.0680906E-4 64.28333 6 CTTTTCT 50 1.7990844E-4 57.855 5 TTTCTTC 55 2.8818485E-4 52.595455 7 TCTTCAA 55 2.8818485E-4 52.595455 9 GATATCA 60 4.4286397E-4 48.212505 145 TTCTTCA 60 4.4286397E-4 48.212505 8 TCAGCCC 45 0.009046344 48.2125 145 CAAAAAT 55 0.0025584647 39.446594 9 TTCAAAA 60 0.004567237 36.159378 7 TCAAAAA 65 0.00776512 33.377884 8 >>END_MODULE SRR8846553 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846553_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.664 33.0 33.0 34.0 32.0 34.0 2 32.89425 33.0 33.0 34.0 32.0 34.0 3 32.894 33.0 33.0 34.0 32.0 34.0 4 32.88425 33.0 33.0 34.0 32.0 34.0 5 32.9105 33.0 33.0 34.0 32.0 34.0 6 37.07275 38.0 38.0 38.0 37.0 38.0 7 37.08025 38.0 38.0 38.0 36.0 38.0 8 37.1065 38.0 38.0 38.0 37.0 38.0 9 37.1985 38.0 38.0 38.0 37.0 38.0 10-14 37.043949999999995 38.0 38.0 38.0 36.2 38.0 15-19 37.003249999999994 38.0 38.0 38.0 36.2 38.0 20-24 37.042500000000004 38.0 38.0 38.0 36.0 38.0 25-29 36.97195 38.0 38.0 38.0 36.0 38.0 30-34 36.9709 38.0 38.0 38.0 36.0 38.0 35-39 36.96295 38.0 38.0 38.0 36.0 38.0 40-44 36.98915000000001 38.0 38.0 38.0 36.0 38.0 45-49 36.953199999999995 38.0 38.0 38.0 36.0 38.0 50-54 36.8001 38.0 38.0 38.0 35.8 38.0 55-59 36.74375 38.0 38.0 38.0 35.6 38.0 60-64 36.6619 38.0 38.0 38.0 35.0 38.0 65-69 36.65415 38.0 38.0 38.0 34.8 38.0 70-74 36.7482 38.0 38.0 38.0 35.2 38.0 75-79 36.5886 38.0 38.0 38.0 34.4 38.0 80-84 36.51315 38.0 38.0 38.0 34.0 38.0 85-89 36.40185 38.0 38.0 38.0 34.0 38.0 90-94 36.36965 38.0 38.0 38.0 34.0 38.0 95-99 36.1206 38.0 38.0 38.0 33.6 38.0 100-104 36.00095 38.0 37.4 38.0 33.4 38.0 105-109 35.82465 38.0 37.0 38.0 32.4 38.0 110-114 35.628550000000004 38.0 37.0 38.0 31.0 38.0 115-119 35.57769999999999 38.0 37.0 38.0 31.2 38.0 120-124 35.412800000000004 38.0 36.4 38.0 30.6 38.0 125-129 35.16055 38.0 36.0 38.0 29.0 38.0 130-134 34.87475 38.0 35.8 38.0 28.0 38.0 135-139 34.45485 38.0 35.0 38.0 25.2 38.0 140-144 33.976749999999996 38.0 35.0 38.0 23.2 38.0 145-149 32.77525000000001 38.0 33.6 38.0 13.6 38.0 150-151 28.537750000000003 35.5 17.5 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 1.0 4 0.0 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 1.0 13 1.0 14 1.0 15 2.0 16 5.0 17 4.0 18 7.0 19 6.0 20 7.0 21 7.0 22 8.0 23 8.0 24 14.0 25 15.0 26 28.0 27 18.0 28 49.0 29 44.0 30 50.0 31 66.0 32 86.0 33 111.0 34 158.0 35 274.0 36 612.0 37 2401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.195439739413683 19.96993234778251 18.140816837885243 41.69381107491857 2 21.70212765957447 20.826032540675847 42.85356695869837 14.618272841051313 3 16.19119119119119 24.324324324324326 40.04004004004004 19.444444444444446 4 21.47147147147147 29.57957957957958 29.72972972972973 19.21921921921922 5 23.329161451814766 33.21652065081352 30.43804755944931 13.016270337922403 6 20.115230460921843 33.54208416833667 29.559118236472948 16.783567134268537 7 16.7711205815994 19.503634996239658 46.15191777387817 17.573326648282777 8 19.01302605210421 24.173346693386772 35.921843687374746 20.89178356713427 9 21.348033074417437 19.669255825607618 38.987722375344525 19.99498872463042 10-14 21.970912738214643 27.4172517552658 31.75526579739218 18.85656970912738 15-19 22.30973762103045 26.709476747102794 32.0624090703858 18.91837656148096 20-24 22.85599719312315 25.718009122349756 32.58483284045913 18.841160844067968 25-29 23.5420949706664 25.943940229654515 31.780574637717496 18.73339016196159 30-34 24.100250626566417 24.641604010025063 31.964912280701753 19.293233082706767 35-39 24.051140636751065 25.394835798445726 30.549009776886436 20.00501378791677 40-44 21.520508839585315 26.368508038263133 31.84754845495067 20.26343466720088 45-49 21.960136217948715 27.363782051282055 30.724158653846157 19.951923076923077 50-54 22.37916353618833 26.616579013273228 31.08940646130729 19.91485098923115 55-59 20.99884786855683 27.215348394529883 30.681761258327906 21.104042478585384 60-64 22.17437027392458 26.506084430867844 30.357053432820873 20.9624918623867 65-69 22.1638129568439 26.57454691098428 30.80004005206769 20.461600080104137 70-74 22.50475523075383 26.45910501551707 31.29942937230954 19.736710381419563 75-79 23.162371778834125 25.4340755566675 31.08831623717788 20.31523642732049 80-84 22.68928589300906 25.66181254065956 32.22238903067608 19.42651253565531 85-89 23.029544316474713 27.43615423134702 29.34401602403605 20.190285428142214 90-94 22.767566484699756 26.669003856363 29.57379676466169 20.98963289427555 95-99 22.369739478957914 26.87875751503006 30.54609218436874 20.205410821643284 100-104 23.602204408817634 26.793587174348698 29.69438877755511 19.909819639278556 105-109 24.668102800460897 25.58489053654627 30.093682681228394 19.65332398176444 110-114 23.772791023842917 25.63614506111 30.68022440392707 19.910839511120017 115-119 22.626201923076923 26.883012820512818 30.36358173076923 20.127203525641026 120-124 22.568853279919878 26.910365548322485 29.26890335503255 21.251877816725088 125-129 23.754942689824315 26.67300665698984 29.736223034185894 19.83582761899995 130-134 22.782338806567882 28.11373648378054 29.37024429315178 19.733680416499798 135-139 24.28399759663529 28.104346084518326 29.010614860805127 18.601041458041255 140-144 24.06628617202363 28.792430159206965 28.92760588765395 18.21367778111545 145-149 24.261392088132197 27.606409614421633 29.173760640961444 18.958437656484726 150-151 26.07879924953096 25.428392745465917 29.593495934959353 18.899312070043777 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 1.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.5 10 0.5 11 0.0 12 0.5 13 0.5 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 3.0 20 4.5 21 5.5 22 6.5 23 8.0 24 11.0 25 14.5 26 27.0 27 32.5 28 31.5 29 41.0 30 58.5 31 63.5 32 67.0 33 78.5 34 117.5 35 155.5 36 152.0 37 198.0 38 233.5 39 225.0 40 216.0 41 228.5 42 245.5 43 239.5 44 222.0 45 191.5 46 157.5 47 118.5 48 102.0 49 80.5 50 61.0 51 49.5 52 32.0 53 31.0 54 32.0 55 22.0 56 15.5 57 16.0 58 24.5 59 32.0 60 43.5 61 42.5 62 25.5 63 34.0 64 48.5 65 49.5 66 34.0 67 13.5 68 10.5 69 9.0 70 4.5 71 2.5 72 8.5 73 8.0 74 2.0 75 2.0 76 2.0 77 1.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.125 3 0.1 4 0.1 5 0.125 6 0.2 7 0.27499999999999997 8 0.2 9 0.22499999999999998 10-14 0.3 15-19 0.335 20-24 0.245 25-29 0.28500000000000003 30-34 0.25 35-39 0.27499999999999997 40-44 0.165 45-49 0.16 50-54 0.17500000000000002 55-59 0.185 60-64 0.155 65-69 0.13 70-74 0.11 75-79 0.075 80-84 0.08499999999999999 85-89 0.15 90-94 0.165 95-99 0.2 100-104 0.2 105-109 0.19499999999999998 110-114 0.18 115-119 0.16 120-124 0.15 125-129 0.105 130-134 0.12 135-139 0.13999999999999999 140-144 0.13 145-149 0.15 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.45 #Duplication Level Percentage of deduplicated Percentage of total 1 86.59902029391182 61.875 2 6.717984604618614 9.6 3 2.3792862141357594 5.1 4 1.4695591322603219 4.2 5 0.874737578726382 3.125 6 0.5248425472358292 2.25 7 0.17494751574527642 0.8750000000000001 8 0.27991602519244224 1.6 9 0.13995801259622112 0.8999999999999999 >10 0.8397480755773268 10.475 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 36 0.8999999999999999 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 31 0.775 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 25 0.625 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 24 0.6 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 24 0.6 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 23 0.575 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 20 0.5 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 18 0.44999999999999996 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 18 0.44999999999999996 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 17 0.42500000000000004 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 16 0.4 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 16 0.4 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 16 0.4 No Hit CGTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGAC 15 0.375 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 14 0.35000000000000003 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 14 0.35000000000000003 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 14 0.35000000000000003 No Hit CTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCAC 13 0.325 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 13 0.325 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 12 0.3 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 10 0.25 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 10 0.25 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 10 0.25 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 10 0.25 No Hit TTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATAC 9 0.22499999999999998 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 9 0.22499999999999998 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 8 0.2 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 8 0.2 No Hit TATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAG 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit CAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTT 7 0.17500000000000002 No Hit ATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGT 7 0.17500000000000002 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 7 0.17500000000000002 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 6 0.15 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 6 0.15 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 6 0.15 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 6 0.15 No Hit AGTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACG 6 0.15 No Hit CAACGATTAAATTGAATTTATGGCTACGCAAACCGTTGAAGATAGTTCTA 6 0.15 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 6 0.15 No Hit CTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACT 6 0.15 No Hit TGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTG 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit GTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGACT 5 0.125 No Hit CGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTAT 5 0.125 No Hit GTGGACTTTGCCGTATGGCTGTGAACCTGGGCCGGGAATGCTATTGATGG 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit CCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGC 5 0.125 No Hit TTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAG 5 0.125 No Hit CTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGAT 5 0.125 No Hit CGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCC 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit CTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAACCATTTATGCG 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 5 0.125 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 5 0.125 No Hit ATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAG 5 0.125 No Hit CATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCG 5 0.125 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit CTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGT 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.2125 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.32499999999999996 0.0 0.0 0.0 0.0 90-91 0.3625 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.525 0.0 0.0 0.0 0.0 96-97 0.6 0.0 0.0 0.0 0.0 98-99 0.675 0.0 0.0 0.0 0.0 100-101 0.75 0.0 0.0 0.0 0.0 102-103 0.85 0.0 0.0 0.0 0.0 104-105 0.975 0.0 0.0 0.0 0.0 106-107 1.1875 0.0 0.0 0.0 0.0 108-109 1.375 0.0 0.0 0.0 0.0 110-111 1.525 0.0 0.0 0.0 0.0 112-113 1.85 0.0 0.0 0.0 0.0 114-115 2.1375 0.0 0.0 0.0 0.0 116-117 2.475 0.0 0.0 0.0 0.0 118-119 2.925 0.0 0.0 0.0 0.0 120-121 3.4625000000000004 0.0 0.0 0.0 0.0 122-123 3.9499999999999997 0.0 0.0 0.0 0.0 124-125 4.5125 0.0 0.0 0.0 0.0 126-127 5.0875 0.0 0.0 0.0 0.0 128-129 5.7125 0.0 0.0 0.0 0.0 130-131 6.4625 0.0 0.0 0.0 0.0 132-133 7.0875 0.0 0.0 0.0 0.0 134-135 7.925000000000001 0.0 0.0 0.0 0.0 136-137 8.649999999999999 0.0 0.0 0.0 0.0 138-139 9.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGATA 10 0.006830828 145.0 1 CTATATT 10 0.006830828 145.0 4 TATATTA 10 0.006830828 145.0 5 ATCTATA 10 0.006830828 145.0 2 TAAGCCA 10 0.006830828 145.0 4 TTAAGCC 10 0.006830828 145.0 3 TATTAAG 10 0.006830828 145.0 1 AAGCCAA 10 0.006830828 145.0 5 TCGGAAG 40 2.9585467E-4 21.75 140-144 CGGAAGA 40 2.9585467E-4 21.75 140-144 >>END_MODULE Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463978 spots for SRR8846553.sra Written 1463978 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra Read 1463959 spots for SRR8846553.sra Written 1463959 spots for SRR8846553.sra SRR ids: ['SRR8846553.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_qr6x198p SRR8846553.sra spots: 29279199 blocks: [[1, 1463959], [1463960, 2927918], [2927919, 4391877], [4391878, 5855836], [5855837, 7319795], [7319796, 8783754], [8783755, 10247713], [10247714, 11711672], [11711673, 13175631], [13175632, 14639590], [14639591, 16103549], [16103550, 17567508], [17567509, 19031467], [19031468, 20495426], [20495427, 21959385], [21959386, 23423344], [23423345, 24887303], [24887304, 26351262], [26351263, 27815221], [27815222, 29279199]] SRR8846553 file size 9900059 SRR8846553 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846553 SRR8846553_1.fastq SRR8846553_2.fastq Input file: SRR8846553_1.fastq Paired file: SRR8846553_2.fastq trimmed: SRR8846553-trimmed-pair1.fastq, SRR8846553-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 10:34:32 2024 >> started Mon Dec 9 10:35:42 2024 >> done (69.379s) 29279199 read pairs processed; of these: 14737 ( 0.05%) short read pairs filtered out after trimming by size control 88472 ( 0.30%) empty read pairs filtered out after trimming by size control 29175990 (99.65%) read pairs available; of these: 13265927 (45.47%) trimmed read pairs available after processing 15910063 (54.53%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 5 0.00% 20 2 0.00% 21 12 0.00% 22 7 0.00% 23 4 0.00% 24 9 0.00% 25 8 0.00% 26 8 0.00% 27 52 0.00% 28 5 0.00% 29 13 0.00% 30 5 0.00% 31 5 0.00% 32 3 0.00% 33 7 0.00% 34 19 0.00% 35 7 0.00% 36 13 0.00% 37 10 0.00% 38 10 0.00% 39 8 0.00% 40 11 0.00% 41 27 0.00% 42 27 0.00% 43 20 0.00% 44 24 0.00% 45 24 0.00% 46 26 0.00% 47 32 0.00% 48 45 0.00% 49 52 0.00% 50 61 0.00% 51 62 0.00% 52 86 0.00% 53 93 0.00% 54 82 0.00% 55 110 0.00% 56 103 0.00% 57 133 0.00% 58 144 0.00% 59 208 0.00% 60 211 0.00% 61 232 0.00% 62 282 0.00% 63 353 0.00% 64 381 0.00% 65 445 0.00% 66 425 0.00% 67 499 0.00% 68 551 0.00% 69 602 0.00% 70 663 0.00% 71 742 0.00% 72 916 0.00% 73 1036 0.00% 74 1244 0.00% 75 1497 0.01% 76 1580 0.01% 77 1781 0.01% 78 1954 0.01% 79 1943 0.01% 80 2384 0.01% 81 2925 0.01% 82 3349 0.01% 83 3867 0.01% 84 4384 0.02% 85 5578 0.02% 86 6228 0.02% 87 6620 0.02% 88 7281 0.02% 89 7568 0.03% 90 9149 0.03% 91 9374 0.03% 92 11025 0.04% 93 12675 0.04% 94 14316 0.05% 95 15162 0.05% 96 16079 0.06% 97 16877 0.06% 98 19148 0.07% 99 21008 0.07% 100 22840 0.08% 101 24781 0.08% 102 27025 0.09% 103 28139 0.10% 104 32100 0.11% 105 37145 0.13% 106 39773 0.14% 107 41680 0.14% 108 42481 0.15% 109 49675 0.17% 110 47726 0.16% 111 55051 0.19% 112 55106 0.19% 113 53144 0.18% 114 57558 0.20% 115 60954 0.21% 116 67058 0.23% 117 71181 0.24% 118 72141 0.25% 119 80465 0.28% 120 80971 0.28% 121 84381 0.29% 122 86836 0.30% 123 86493 0.30% 124 93364 0.32% 125 107026 0.37% 126 102352 0.35% 127 111237 0.38% 128 121182 0.42% 129 133561 0.46% 130 128867 0.44% 131 143630 0.49% 132 134478 0.46% 133 137508 0.47% 134 144873 0.50% 135 143871 0.49% 136 159276 0.55% 137 162472 0.56% 138 180904 0.62% 139 186338 0.64% 140 191725 0.66% 141 223661 0.77% 142 207405 0.71% 143 234417 0.80% 144 257899 0.88% 145 311926 1.07% 146 332999 1.14% 147 416915 1.43% 148 586357 2.01% 149 1078184 3.70% 150 5714874 19.59% 151 15910063 54.53% 29175990 reads passed initial QC criterion=sequence-density sequence-density=4.20 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=34 prefix-density=4.08 prefix-fanout=1.9 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=1496.52 fanout-score-rank=1 prefix-density=12.39 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=2.43 sequence-density-rank=1 fanout-score=1.75 fanout-score-rank=38 prefix-density=4.22 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=66.65 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=8.1 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x CCAGCCTCACGC -y TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG -o SRR8846553 SRR8846553_1.fastq SRR8846553_2.fastq Input file: SRR8846553_1.fastq Paired file: SRR8846553_2.fastq trimmed: SRR8846553-trimmed-pair1.fastq, SRR8846553-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): CCAGCCTCACGC -- paired 3' end adapter sequence (-y): TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Dec 9 10:55:22 2024 >> started Mon Dec 9 10:55:49 2024 >> done (27.027s) 14587995 read pairs processed; of these: 2712 ( 0.02%) short read pairs filtered out after trimming by size control 6789 ( 0.05%) empty read pairs filtered out after trimming by size control 14578494 (99.93%) read pairs available; of these: 2375 ( 0.02%) trimmed read pairs available after processing 14576119 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 1 0.00% 20 1 0.00% 21 3 0.00% 22 2 0.00% 23 3 0.00% 24 5 0.00% 25 3 0.00% 26 4 0.00% 27 24 0.00% 28 2 0.00% 29 6 0.00% 30 1 0.00% 31 2 0.00% 32 2 0.00% 33 6 0.00% 34 7 0.00% 35 3 0.00% 36 7 0.00% 37 3 0.00% 38 8 0.00% 39 4 0.00% 40 5 0.00% 41 12 0.00% 42 18 0.00% 43 5 0.00% 44 15 0.00% 45 12 0.00% 46 16 0.00% 47 12 0.00% 48 24 0.00% 49 24 0.00% 50 35 0.00% 51 37 0.00% 52 45 0.00% 53 43 0.00% 54 43 0.00% 55 48 0.00% 56 47 0.00% 57 64 0.00% 58 85 0.00% 59 108 0.00% 60 93 0.00% 61 106 0.00% 62 153 0.00% 63 171 0.00% 64 173 0.00% 65 212 0.00% 66 218 0.00% 67 245 0.00% 68 277 0.00% 69 307 0.00% 70 327 0.00% 71 379 0.00% 72 485 0.00% 73 531 0.00% 74 614 0.00% 75 748 0.01% 76 795 0.01% 77 873 0.01% 78 1019 0.01% 79 986 0.01% 80 1211 0.01% 81 1434 0.01% 82 1646 0.01% 83 1935 0.01% 84 2175 0.01% 85 2757 0.02% 86 3121 0.02% 87 3349 0.02% 88 3588 0.02% 89 3680 0.03% 90 4613 0.03% 91 4695 0.03% 92 5498 0.04% 93 6409 0.04% 94 7140 0.05% 95 7611 0.05% 96 8143 0.06% 97 8436 0.06% 98 9663 0.07% 99 10474 0.07% 100 11548 0.08% 101 12412 0.09% 102 13522 0.09% 103 14043 0.10% 104 15828 0.11% 105 18365 0.13% 106 19855 0.14% 107 20820 0.14% 108 21146 0.15% 109 24854 0.17% 110 23785 0.16% 111 27331 0.19% 112 27396 0.19% 113 26405 0.18% 114 28677 0.20% 115 30446 0.21% 116 33340 0.23% 117 35518 0.24% 118 35823 0.25% 119 40289 0.28% 120 40605 0.28% 121 41823 0.29% 122 43370 0.30% 123 43249 0.30% 124 46730 0.32% 125 53219 0.37% 126 51010 0.35% 127 55242 0.38% 128 60622 0.42% 129 67027 0.46% 130 64308 0.44% 131 71532 0.49% 132 67251 0.46% 133 68600 0.47% 134 72562 0.50% 135 72000 0.49% 136 79935 0.55% 137 81340 0.56% 138 90868 0.62% 139 93231 0.64% 140 95906 0.66% 141 111886 0.77% 142 103752 0.71% 143 117729 0.81% 144 128641 0.88% 145 156018 1.07% 146 166173 1.14% 147 208399 1.43% 148 292539 2.01% 149 538265 3.69% 150 2854805 19.58% 151 7951364 54.54% criterion=sequence-density sequence-density=4.08 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=36 prefix-density=4.02 prefix-fanout=1.9 sequence=CCAGCCTCACGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=1797.73 fanout-score-rank=1 prefix-density=12.56 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=2.39 sequence-density-rank=1 fanout-score=1.74 fanout-score-rank=38 prefix-density=4.12 prefix-fanout=1.0 sequence=TTGCGTAGTGGGCCGTTGGAGCTTAAGCGAAAGCTAGGCTTCGCGGCCCATAATGTTGGCAGGCACAGCGTGAGGCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=135.28 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=2.0 sequence=TATAGTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACTGAATTTAAAATGATATACTTAAGGCATCCTTAAGTTTTTTTTCTATTCCGATGAAAACTTTAGTTCTTATAAAGGATTTCATCCTTTTCCTCTCAATAGCGTATTGAGGAACAATATACATTCTCGTGATTTGTATAATTGAAATTGCATCCAAAATACAAATAGGATTATGAGTACAGAGTCGCGAAGCATAATTTTACATTGGATTAAGTATTCCATATATTAAAAATATGAGTAA SRR8846553 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 09 11:00:18 Started mapping on | Dec 09 11:00:18 Finished on | Dec 09 11:04:12 Mapping speed, Million of reads per hour | 448.72 Number of input reads | 29166489 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 18292100 Uniquely mapped reads % | 62.72% Average mapped length | 294.04 Number of splices: Total | 3389667 Number of splices: Annotated (sjdb) | 2998257 Number of splices: GT/AG | 3205488 Number of splices: GC/AG | 37107 Number of splices: AT/AC | 20578 Number of splices: Non-canonical | 126494 Mismatch rate per base, % | 0.20% Deletion rate per base | 0.01% Deletion average length | 1.57 Insertion rate per base | 0.02% Insertion average length | 1.87 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9507419 % of reads mapped to multiple loci | 32.60% Number of reads mapped to too many loci | 6085 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.74% % of reads unmapped: other | 0.93% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1373405 1373405 1373405 N_multimapping 9507419 9507419 9507419 N_noFeature 4608427 17511046 4913183 N_ambiguous 1046396 17299 584720 UnstrandedReadsAssigned:12637277 PositiveStrandReadsAssigned:763755 NegativeStrandReadsAssigned:12794197 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR8846553 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846553-trimmed-pair1.fastq SRR8846553-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,166,489 reads, 17,976,246 reads pseudoaligned [quant] estimated average fragment length: 214.798 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,019 rounds 52973 SRR8846553.ke.tsv 35125 SRR8846553.se.tsv 88098 total ==> SRR8846553.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 723.153 0 0 PNS24247 1044 830.202 8.812 0.586156 PNS24249 1928 1714.2 28.3851 0.914432 PNS24246 1044 830.202 8.812 0.586156 PNS24248 1044 830.202 8.812 0.586156 PNS24244 1471 1257.2 28.1789 1.23777 PNS24243 293 107.255 0 0 KQK14069 1603 1389.2 1614.5 64.1793 KQK14071 474 267.294 48.3376 9.98661 ==> SRR8846553.se.tsv <== BRADI_1g14170v3 2304 BRADI_1g53295v3 12 BRADI_1g59795v3 66 BRADI_1g07683v3 0 BRADI_1g00485v3 11 BRADI_1g20270v3 495 BRADI_1g74790v3 7 BRADI_1g09890v3 1 BRADI_1g77505v3 47 BRADI_1g48960v3 0 SRR8846553 completed mapping pipeline successfully