Starting /dee2/code/volunteer_pipeline.sh SRR8846559 current disk space = 1515199430656 free memory = 1591391328 SRR8846559 SRAfilesize b12faed5e5f480b0779eff3c027fc0b4 SRR8846559.sra SRR8846559.sra file validated SRR8846559 is paired end SRR8846559 is conventional basespace SRR8846559 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846559_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.25975 34.0 33.0 34.0 33.0 34.0 2 33.29275 34.0 33.0 34.0 32.0 34.0 3 33.3275 34.0 33.0 34.0 33.0 34.0 4 33.20625 34.0 33.0 34.0 32.0 34.0 5 33.21725 34.0 33.0 34.0 33.0 34.0 6 36.734 38.0 37.0 38.0 34.0 38.0 7 37.15975 38.0 38.0 38.0 36.0 38.0 8 37.17975 38.0 38.0 38.0 36.0 38.0 9 37.33575 38.0 38.0 38.0 37.0 38.0 10-14 37.2575 38.0 38.0 38.0 36.6 38.0 15-19 37.2548 38.0 38.0 38.0 36.4 38.0 20-24 37.362 38.0 38.0 38.0 37.0 38.0 25-29 37.2935 38.0 38.0 38.0 36.8 38.0 30-34 37.24435 38.0 38.0 38.0 37.0 38.0 35-39 37.22325 38.0 38.0 38.0 36.2 38.0 40-44 37.228300000000004 38.0 38.0 38.0 36.6 38.0 45-49 37.21015 38.0 38.0 38.0 36.6 38.0 50-54 37.10815 38.0 38.0 38.0 36.0 38.0 55-59 36.61280000000001 38.0 38.0 38.0 35.0 38.0 60-64 35.655150000000006 38.0 38.0 38.0 33.6 38.0 65-69 36.522 38.0 38.0 38.0 34.0 38.0 70-74 36.9602 38.0 38.0 38.0 36.0 38.0 75-79 36.92935 38.0 38.0 38.0 35.6 38.0 80-84 36.76495 38.0 38.0 38.0 35.0 38.0 85-89 36.689949999999996 38.0 38.0 38.0 34.6 38.0 90-94 36.58030000000001 38.0 38.0 38.0 34.2 38.0 95-99 36.4753 38.0 38.0 38.0 34.0 38.0 100-104 36.24225 38.0 38.0 38.0 33.6 38.0 105-109 36.209700000000005 38.0 37.8 38.0 33.2 38.0 110-114 36.059749999999994 38.0 37.0 38.0 32.6 38.0 115-119 35.96925 38.0 37.0 38.0 32.6 38.0 120-124 35.7244 38.0 37.0 38.0 31.0 38.0 125-129 35.298 38.0 36.2 38.0 29.2 38.0 130-134 35.06425 38.0 35.8 38.0 28.6 38.0 135-139 34.463049999999996 38.0 34.8 38.0 24.6 38.0 140-144 33.8947 38.0 33.2 38.0 22.8 38.0 145-149 33.35295000000001 38.0 33.0 38.0 19.8 38.0 150-151 29.329500000000003 35.5 27.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 1.0 16 0.0 17 0.0 18 2.0 19 2.0 20 0.0 21 2.0 22 2.0 23 10.0 24 14.0 25 21.0 26 20.0 27 30.0 28 29.0 29 62.0 30 55.0 31 78.0 32 98.0 33 123.0 34 204.0 35 331.0 36 539.0 37 2376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.849999999999998 9.4 9.075 57.675 2 18.325 12.425 43.25 26.0 3 16.55 16.525000000000002 29.799999999999997 37.125 4 22.15 24.0 26.575 27.275 5 22.455613903475868 29.35733933483371 29.457364341085274 18.72968242060515 6 17.175 35.175 29.525000000000002 18.125 7 12.725 27.6 44.375 15.299999999999999 8 16.575 25.575 39.1 18.75 9 16.975 22.725 39.300000000000004 21.0 10-14 18.625 33.445 26.345000000000002 21.584999999999997 15-19 19.068580861387623 31.80431194037317 28.097643939772897 21.02946325846631 20-24 18.490000000000002 31.195 29.26 21.055 25-29 20.375 30.880000000000003 28.355000000000004 20.39 30-34 21.7 31.0 26.31 20.990000000000002 35-39 20.895 32.32 26.215 20.57 40-44 18.845 30.669999999999998 28.139999999999997 22.345000000000002 45-49 18.84 30.685000000000002 28.294999999999998 22.18 50-54 18.455 32.2 28.115000000000002 21.23 55-59 20.726279587295167 30.492615820352015 26.239126036819744 22.541978555533078 60-64 18.48582407311247 31.67514799044553 27.811818465053484 22.027209471388513 65-69 20.469748025951816 31.806065483075997 26.037318312125933 21.68686817884625 70-74 21.14 30.925000000000004 25.509999999999998 22.425 75-79 20.735 30.7 27.105 21.46 80-84 21.735 29.835 27.155 21.275 85-89 21.86 30.605 26.375 21.16 90-94 20.78 30.97 27.505000000000003 20.745 95-99 20.825 31.130000000000003 26.640000000000004 21.404999999999998 100-104 19.505 31.869999999999997 26.555 22.07 105-109 20.255000000000003 28.485 28.715000000000003 22.545 110-114 20.04 29.580000000000002 27.965 22.415 115-119 19.139999999999997 30.29 27.52 23.05 120-124 17.815 31.5 26.979999999999997 23.705000000000002 125-129 19.960998049902496 32.48162408120406 24.8262413120656 22.731136556827842 130-134 22.24 31.61 24.94 21.21 135-139 22.431121556077805 30.781539076953845 24.64123206160308 22.146107305365266 140-144 22.29 30.79 26.284999999999997 20.635 145-149 20.755000000000003 30.935000000000002 25.94 22.37 150-151 21.0375 31.587500000000002 25.224999999999998 22.15 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 1.5 20 2.5 21 4.5 22 8.5 23 10.5 24 12.5 25 14.5 26 18.5 27 24.0 28 34.0 29 43.0 30 49.5 31 58.5 32 73.5 33 77.5 34 93.5 35 127.0 36 164.0 37 278.5 38 310.0 39 215.5 40 209.5 41 221.0 42 214.5 43 245.5 44 232.5 45 202.0 46 161.5 47 115.5 48 102.0 49 78.5 50 63.5 51 50.0 52 37.5 53 38.0 54 33.0 55 35.0 56 32.0 57 23.0 58 30.0 59 35.0 60 32.0 61 24.5 62 17.0 63 15.5 64 24.0 65 33.5 66 23.5 67 10.0 68 5.0 69 4.0 70 5.0 71 7.0 72 5.0 73 1.5 74 2.5 75 3.0 76 1.0 77 0.0 78 1.5 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.025 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.045 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.1400000000000001 60-64 3.71 65-69 0.585 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.005 130-134 0.0 135-139 0.005 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.25 #Duplication Level Percentage of deduplicated Percentage of total 1 87.6470588235294 63.324999999999996 2 6.401384083044983 9.25 3 2.387543252595156 5.175 4 1.3494809688581315 3.9 5 0.4152249134948097 1.5 6 0.34602076124567477 1.5 7 0.34602076124567477 1.7500000000000002 8 0.2422145328719723 1.4000000000000001 9 0.20761245674740486 1.35 >10 0.5882352941176471 7.249999999999999 >50 0.06920415224913494 3.5999999999999996 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 79 1.975 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 65 1.625 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 32 0.8 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 30 0.75 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 26 0.65 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 24 0.6 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 20 0.5 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 19 0.475 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 18 0.44999999999999996 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 16 0.4 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 15 0.375 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 14 0.35000000000000003 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 12 0.3 No Hit GTGCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCG 12 0.3 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 11 0.27499999999999997 No Hit TTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATA 11 0.27499999999999997 No Hit CAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAA 10 0.25 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 10 0.25 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 10 0.25 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 9 0.22499999999999998 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 9 0.22499999999999998 No Hit CTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCGGCCCGT 9 0.22499999999999998 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 9 0.22499999999999998 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 9 0.22499999999999998 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 8 0.2 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 8 0.2 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 8 0.2 No Hit GCTAGTTATCCAGTTGCAGAAGCGACCCCACAGGCTTGTACTTTCGCGTC 8 0.2 No Hit CTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGA 8 0.2 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 8 0.2 No Hit CCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCG 8 0.2 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 7 0.17500000000000002 No Hit GTAGGGATCATCAAAACACCGAACCATCCGATGTAAAGACGATTTTCAGT 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit CTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAA 7 0.17500000000000002 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTA 7 0.17500000000000002 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 7 0.17500000000000002 No Hit GTGGTTCTTTGGAGTAGGCTGTGAGACCTAAGCGGGTCAGGAATGCAGCG 7 0.17500000000000002 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 7 0.17500000000000002 No Hit CTCGGTTTATCGAATGCTGATACATAGTGCAATATGGTCAGAACAGGGTG 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 6 0.15 No Hit TTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGG 6 0.15 No Hit CAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAA 6 0.15 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 6 0.15 No Hit CGGTAAAACAGATCAAACAGATTATTATCGAAATGATTCGAACTGTTTCA 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit CCCAATTTTGGCTTAATAGTACATCCCAATAAAGGACGACCATACTTGTT 6 0.15 No Hit CTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAG 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit CTTTTTTTGATTGTCTGTCAATCAATATTCTAATGGCAATGCAATTTCAT 5 0.125 No Hit CCCCTTCTAACTTACCTACTACTGTACCGGCGTGGATATGATCTCCCCCA 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit GGGTAAACCACCGCCTCTCAGGCCTCCCCGACGGGTTCTACCATAGAGGC 5 0.125 No Hit CCCGAACACAGCTTACAACTTTCATCGTACTGTGCTCTCCAAAGAGCAAC 5 0.125 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit CCCTACGATCCAACCAATTGGGAGAGAATCAATAGACTCCTTTTCGGGAG 5 0.125 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit GGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGA 5 0.125 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.4375 0.0 0.0 0.0 0.0 92-93 0.5249999999999999 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.8 0.0 0.0 0.0 0.0 98-99 0.875 0.0 0.0 0.0 0.0 100-101 1.075 0.0 0.0 0.0 0.0 102-103 1.3375 0.0 0.0 0.0 0.0 104-105 1.675 0.0 0.0 0.0 0.0 106-107 1.9375 0.0 0.0 0.0 0.0 108-109 2.1624999999999996 0.0 0.0 0.0 0.0 110-111 2.4125 0.0 0.0 0.0 0.0 112-113 2.675 0.0 0.0 0.0 0.0 114-115 3.05 0.0 0.0 0.0 0.0 116-117 3.525 0.0 0.0 0.0 0.0 118-119 4.125 0.0 0.0 0.0 0.0 120-121 4.6625 0.0 0.0 0.0 0.0 122-123 5.2875 0.0 0.0 0.0 0.0 124-125 5.7875 0.0 0.0 0.0 0.0 126-127 6.5375 0.0 0.0 0.0 0.0 128-129 7.475 0.0 0.0 0.0 0.0 130-131 8.3125 0.0 0.0 0.0 0.0 132-133 9.212499999999999 0.0 0.0 0.0 0.0 134-135 10.149999999999999 0.0 0.0 0.0 0.0 136-137 10.875 0.0 0.0 0.0 0.0 138-139 11.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTTC 10 0.00686971 144.72499 1 CAGCTTT 10 0.00686971 144.72499 145 CTTTCTT 25 5.7586058E-6 115.78 1 TCTTTTC 30 1.4252144E-5 96.48334 4 CTTTTCT 35 3.0638752E-5 82.7 5 TTTCTTT 35 3.0638752E-5 82.7 2 TTTCTTC 35 3.0638752E-5 82.7 7 TTCTTTT 35 3.0638752E-5 82.7 3 TTCTTCA 35 3.0638752E-5 82.7 8 TTTTCTT 40 5.9413862E-5 72.362495 6 TCTTCAA 40 5.9413862E-5 72.362495 9 GATATCA 50 1.7937113E-4 57.89 145 CTTCAAA 35 0.0035757453 20.675 6 AAATTCT 35 0.0035757453 20.675 4 TATGTTA 35 0.0035757453 20.675 25-29 TGTTAGC 35 0.0035757453 20.675 25-29 TTCTTAT 35 0.0035757453 20.675 7 CTTATAT 35 0.0035757453 20.675 9 ATTCTTA 35 0.0035757453 20.675 6 TCTTATA 35 0.0035757453 20.675 8 >>END_MODULE SRR8846559 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR8846559_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56925 33.0 33.0 34.0 32.0 34.0 2 32.84675 33.0 33.0 34.0 32.0 34.0 3 32.83525 33.0 33.0 34.0 32.0 34.0 4 32.82025 33.0 33.0 34.0 32.0 34.0 5 32.76925 33.0 33.0 34.0 32.0 34.0 6 36.81975 38.0 38.0 38.0 35.0 38.0 7 37.019 38.0 38.0 38.0 36.0 38.0 8 36.94875 38.0 38.0 38.0 36.0 38.0 9 36.98475 38.0 38.0 38.0 36.0 38.0 10-14 36.9199 38.0 38.0 38.0 36.0 38.0 15-19 36.908 38.0 38.0 38.0 36.0 38.0 20-24 36.861149999999995 38.0 38.0 38.0 36.0 38.0 25-29 36.79445 38.0 38.0 38.0 35.6 38.0 30-34 36.82345 38.0 38.0 38.0 35.4 38.0 35-39 36.82455 38.0 38.0 38.0 36.0 38.0 40-44 36.793899999999994 38.0 38.0 38.0 35.4 38.0 45-49 36.71405 38.0 38.0 38.0 35.2 38.0 50-54 36.62825 38.0 38.0 38.0 34.8 38.0 55-59 36.6311 38.0 38.0 38.0 34.6 38.0 60-64 36.483450000000005 38.0 38.0 38.0 34.2 38.0 65-69 36.4897 38.0 38.0 38.0 34.2 38.0 70-74 36.46485 38.0 38.0 38.0 34.0 38.0 75-79 36.43435 38.0 38.0 38.0 34.0 38.0 80-84 36.33215 38.0 38.0 38.0 34.0 38.0 85-89 36.23585 38.0 38.0 38.0 33.6 38.0 90-94 36.0546 38.0 37.8 38.0 33.0 38.0 95-99 35.945 38.0 37.2 38.0 33.0 38.0 100-104 35.7799 38.0 37.0 38.0 31.6 38.0 105-109 35.57084999999999 38.0 37.0 38.0 30.6 38.0 110-114 35.371449999999996 38.0 36.6 38.0 29.6 38.0 115-119 35.3365 38.0 36.6 38.0 29.8 38.0 120-124 35.07195 38.0 36.0 38.0 28.2 38.0 125-129 34.61295 38.0 35.0 38.0 26.0 38.0 130-134 34.450300000000006 38.0 35.0 38.0 25.6 38.0 135-139 34.00535 38.0 35.0 38.0 23.2 38.0 140-144 33.46095 38.0 34.8 38.0 18.8 38.0 145-149 32.1345 38.0 33.2 38.0 8.8 38.0 150-151 27.766750000000002 35.0 17.0 38.0 2.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 1.0 4 2.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 0.0 11 0.0 12 3.0 13 3.0 14 4.0 15 1.0 16 3.0 17 7.0 18 5.0 19 5.0 20 7.0 21 9.0 22 15.0 23 11.0 24 21.0 25 22.0 26 30.0 27 35.0 28 46.0 29 64.0 30 66.0 31 77.0 32 81.0 33 144.0 34 159.0 35 281.0 36 582.0 37 2304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.408521303258144 20.576441102756892 16.917293233082706 39.097744360902254 2 21.331997996995494 20.756134201301954 43.114672008012015 14.797195793690534 3 16.975463194792187 24.611917876815223 38.15723585378067 20.255383074611917 4 21.156735102653982 30.54581872809214 29.193790686029043 19.103655483224838 5 22.358537806710068 33.97596394591888 29.193790686029043 14.471707561342011 6 19.033308289506635 35.712496869521665 30.35311795642374 14.901076884547958 7 17.147154675357232 19.42842817748809 45.29957382802707 18.1248433191276 8 19.564128256513026 24.724448897795593 34.79458917835671 20.916833667334668 9 20.476190476190474 21.027568922305765 38.796992481203006 19.69924812030075 10-14 22.05675892498997 28.43461692739671 30.93662254312074 18.57200160449258 15-19 23.113183892482823 26.794042425154206 30.931247179178577 19.161526503184394 20-24 22.541353383458645 27.097744360902254 31.413533834586467 18.947368421052634 25-29 22.445603128446805 26.902637120224604 31.108994284568336 19.542765466760255 30-34 23.239260113288886 26.592811669757882 31.229635570705298 18.93829264624793 35-39 22.960846242542736 26.886248558680503 30.255176216974984 19.897728981801773 40-44 21.70624185953311 27.607454162909526 30.76846007414087 19.91784390341649 45-49 22.24837255883826 28.96845267901853 29.594391587381068 19.188783174762143 50-54 21.416407893418814 27.777221276169488 31.07783231493539 19.72853851547631 55-59 20.69829183990382 28.40254470770926 30.125732605319843 20.773430847067072 60-64 21.87280921382073 27.496244366549828 30.741111667501254 19.88983475212819 65-69 21.337005508262394 27.315973960941413 30.57085628442664 20.776164246369554 70-74 22.04807210816224 27.521281922884327 31.352028042063097 19.078617926890335 75-79 22.115432747659806 27.026079991990787 31.321019172047855 19.537468088301544 80-84 22.296214700580812 26.792509513318645 32.250150210294414 18.66112557580613 85-89 21.87280921382073 28.052078117175768 29.53430145217827 20.54081121682524 90-94 22.468195933086246 27.06601222077532 30.221376339777624 20.244415506360813 95-99 21.649381231524625 27.786963274713163 30.201913923543263 20.36174157021895 100-104 22.767812406052713 27.31736646958613 30.714500450946993 19.20032067341417 105-109 23.468416570655712 26.458949055753145 30.601612984020438 19.471021389570705 110-114 23.036465638148666 27.36425566018834 30.549989981967542 19.04928871969545 115-119 21.918357124968697 28.279489105935383 29.86225895316804 19.939894815927875 120-124 22.8943415122684 27.986980470706058 29.01352028042063 20.105157736604905 125-129 23.695543314972458 27.87681522283425 28.983475212819226 19.444166249374064 130-134 22.784176264396592 28.30746119178768 29.158738107160744 19.749624436654983 135-139 24.067304321698632 28.18368471130252 28.879763633632127 18.86924733336672 140-144 24.261392088132197 27.811717576364547 29.389083625438158 18.537806710065098 145-149 24.82471955128205 27.764423076923077 28.595753205128204 18.815104166666664 150-151 25.369026770077557 27.24543407555667 29.459594696022016 17.925944458343757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 2.0 2 0.5 3 0.5 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 3.5 19 5.0 20 3.0 21 4.0 22 4.5 23 8.0 24 11.0 25 18.0 26 34.0 27 37.0 28 31.5 29 43.0 30 62.5 31 79.0 32 89.0 33 93.0 34 126.5 35 167.0 36 167.0 37 191.0 38 220.5 39 209.5 40 219.0 41 233.0 42 229.5 43 241.0 44 217.5 45 170.0 46 149.5 47 131.5 48 108.5 49 82.5 50 71.5 51 60.5 52 37.5 53 31.0 54 33.0 55 26.5 56 22.0 57 17.0 58 18.5 59 33.5 60 41.5 61 34.5 62 25.0 63 25.0 64 25.5 65 28.0 66 24.0 67 7.5 68 3.0 69 7.0 70 8.5 71 5.5 72 5.5 73 5.5 74 2.5 75 2.0 76 2.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.15 3 0.15 4 0.15 5 0.15 6 0.17500000000000002 7 0.27499999999999997 8 0.2 9 0.25 10-14 0.27999999999999997 15-19 0.295 20-24 0.25 25-29 0.27 30-34 0.255 35-39 0.265 40-44 0.19 45-49 0.15 50-54 0.16999999999999998 55-59 0.185 60-64 0.15 65-69 0.15 70-74 0.15 75-79 0.11499999999999999 80-84 0.13999999999999999 85-89 0.15 90-94 0.16999999999999998 95-99 0.20500000000000002 100-104 0.21 105-109 0.185 110-114 0.18 115-119 0.17500000000000002 120-124 0.15 125-129 0.15 130-134 0.15 135-139 0.155 140-144 0.15 145-149 0.16 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.425 #Duplication Level Percentage of deduplicated Percentage of total 1 86.74179648657608 65.425 2 7.55717600265164 11.4 3 2.0218760357971495 4.575 4 1.4584023864766325 4.3999999999999995 5 0.596619158104077 2.25 6 0.46403712296983757 2.1 7 0.23201856148491878 1.225 8 0.16572754391779915 1.0 9 0.1325820351342393 0.8999999999999999 >10 0.6297646668876367 6.7250000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 26 0.65 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 26 0.65 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 24 0.6 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 19 0.475 No Hit GTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACTGAATTTAAAATG 17 0.42500000000000004 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 13 0.325 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 13 0.325 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 12 0.3 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 12 0.3 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 12 0.3 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 12 0.3 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 12 0.3 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 11 0.27499999999999997 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 10 0.25 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 10 0.25 No Hit CGTTAGCGTGAGCTTGTAACCCGAGTGGGGGCATTAAGGGTGGCGTGGAC 10 0.25 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 10 0.25 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 10 0.25 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 10 0.25 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 9 0.22499999999999998 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 8 0.2 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 8 0.2 No Hit CATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTC 8 0.2 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 8 0.2 No Hit TTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGT 7 0.17500000000000002 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 7 0.17500000000000002 No Hit CTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAG 7 0.17500000000000002 No Hit CTCAGTTCGGTAGAGCAACTCGTTTACACGTGCGCCAATGCTTTTCAAAG 7 0.17500000000000002 No Hit TGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTC 7 0.17500000000000002 No Hit CTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGGGT 7 0.17500000000000002 No Hit CTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGC 7 0.17500000000000002 No Hit CAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTT 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 6 0.15 No Hit ATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAAT 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit AGCTCAGTTGGTAGAGCTCCGCTCTTGCAATTGGGTCGTTGCGATTACGG 6 0.15 No Hit CTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATG 6 0.15 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 6 0.15 No Hit CAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAACGTAATGCTCACA 6 0.15 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 6 0.15 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6 0.15 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 5 0.125 No Hit GCTCAGTTCGGTAGAGCAACTCGTTTACACGTGCGCCAATGCTTTTCAAA 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 5 0.125 No Hit CTTTGGTACAAAATTGACAATCTCACAAGGATGAAATACCAGTAATTTTT 5 0.125 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit GTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGGA 5 0.125 No Hit CTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTT 5 0.125 No Hit CGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGGTATGG 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit GAGCAACTCGTTTACACGTGCGCCAATGCTTTTCAAAGGAGCTTATTATG 5 0.125 No Hit CCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATA 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.4375 0.0 0.0 0.0 0.0 92-93 0.5249999999999999 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.8 0.0 0.0 0.0 0.0 98-99 0.875 0.0 0.0 0.0 0.0 100-101 1.075 0.0 0.0 0.0 0.0 102-103 1.3375 0.0 0.0 0.0 0.0 104-105 1.6375 0.0 0.0 0.0 0.0 106-107 1.9125 0.0 0.0 0.0 0.0 108-109 2.1375 0.0 0.0 0.0 0.0 110-111 2.4000000000000004 0.0 0.0 0.0 0.0 112-113 2.6624999999999996 0.0 0.0 0.0 0.0 114-115 3.025 0.0 0.0 0.0 0.0 116-117 3.525 0.0 0.0 0.0 0.0 118-119 4.125 0.0 0.0 0.0 0.0 120-121 4.675000000000001 0.0 0.0 0.0 0.0 122-123 5.3125 0.0 0.0 0.0 0.0 124-125 5.8125 0.0 0.0 0.0 0.0 126-127 6.5625 0.0 0.0 0.0 0.0 128-129 7.475 0.0 0.0 0.0 0.0 130-131 8.287500000000001 0.0 0.0 0.0 0.0 132-133 9.1875 0.0 0.0 0.0 0.0 134-135 10.149999999999999 0.0 0.0 0.0 0.0 136-137 10.8875 0.0 0.0 0.0 0.0 138-139 11.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATGGTT 35 0.0033124194 62.14286 145 >>END_MODULE Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra Read 1654769 spots for SRR8846559.sra Written 1654769 spots for SRR8846559.sra SRR ids: ['SRR8846559.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_za3iehts SRR8846559.sra spots: 33095380 blocks: [[1, 1654769], [1654770, 3309538], [3309539, 4964307], [4964308, 6619076], [6619077, 8273845], [8273846, 9928614], [9928615, 11583383], [11583384, 13238152], [13238153, 14892921], [14892922, 16547690], [16547691, 18202459], [18202460, 19857228], [19857229, 21511997], [21511998, 23166766], [23166767, 24821535], [24821536, 26476304], [26476305, 28131073], [28131074, 29785842], [29785843, 31440611], [31440612, 33095380]] SRR8846559 file size 11193237 SRR8846559 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR8846559 SRR8846559_1.fastq SRR8846559_2.fastq Input file: SRR8846559_1.fastq Paired file: SRR8846559_2.fastq trimmed: SRR8846559-trimmed-pair1.fastq, SRR8846559-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 03:14:19 2024 >> started Thu Dec 12 03:18:24 2024 >> done (245.316s) 33095380 read pairs processed; of these: 13596 ( 0.04%) short read pairs filtered out after trimming by size control 105064 ( 0.32%) empty read pairs filtered out after trimming by size control 32976720 (99.64%) read pairs available; of these: 14433591 (43.77%) trimmed read pairs available after processing 18543129 (56.23%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 6 0.00% 20 7 0.00% 21 9 0.00% 22 6 0.00% 23 5 0.00% 24 5 0.00% 25 12 0.00% 26 11 0.00% 27 31 0.00% 28 10 0.00% 29 5 0.00% 30 14 0.00% 31 8 0.00% 32 12 0.00% 33 14 0.00% 34 17 0.00% 35 25 0.00% 36 10 0.00% 37 25 0.00% 38 20 0.00% 39 21 0.00% 40 26 0.00% 41 31 0.00% 42 35 0.00% 43 35 0.00% 44 44 0.00% 45 51 0.00% 46 54 0.00% 47 65 0.00% 48 69 0.00% 49 77 0.00% 50 92 0.00% 51 98 0.00% 52 147 0.00% 53 137 0.00% 54 152 0.00% 55 189 0.00% 56 205 0.00% 57 222 0.00% 58 234 0.00% 59 302 0.00% 60 311 0.00% 61 372 0.00% 62 435 0.00% 63 503 0.00% 64 587 0.00% 65 659 0.00% 66 752 0.00% 67 769 0.00% 68 963 0.00% 69 991 0.00% 70 1166 0.00% 71 1268 0.00% 72 1464 0.00% 73 1746 0.01% 74 1994 0.01% 75 2453 0.01% 76 2602 0.01% 77 2924 0.01% 78 3046 0.01% 79 3419 0.01% 80 4051 0.01% 81 4560 0.01% 82 5072 0.02% 83 5874 0.02% 84 6818 0.02% 85 8462 0.03% 86 9345 0.03% 87 10043 0.03% 88 10699 0.03% 89 11496 0.03% 90 13667 0.04% 91 14155 0.04% 92 16666 0.05% 93 18516 0.06% 94 20867 0.06% 95 22858 0.07% 96 23392 0.07% 97 25115 0.08% 98 28460 0.09% 99 31288 0.09% 100 33835 0.10% 101 36759 0.11% 102 40281 0.12% 103 42344 0.13% 104 46393 0.14% 105 54275 0.16% 106 57215 0.17% 107 58617 0.18% 108 60344 0.18% 109 70569 0.21% 110 70553 0.21% 111 76730 0.23% 112 77855 0.24% 113 74341 0.23% 114 78885 0.24% 115 82216 0.25% 116 90721 0.28% 117 96696 0.29% 118 96066 0.29% 119 106117 0.32% 120 107777 0.33% 121 112049 0.34% 122 114981 0.35% 123 113893 0.35% 124 123804 0.38% 125 137506 0.42% 126 133456 0.40% 127 141610 0.43% 128 150544 0.46% 129 161747 0.49% 130 156262 0.47% 131 171976 0.52% 132 161092 0.49% 133 162168 0.49% 134 169761 0.51% 135 168355 0.51% 136 181996 0.55% 137 183470 0.56% 138 201128 0.61% 139 208709 0.63% 140 213797 0.65% 141 241069 0.73% 142 228680 0.69% 143 255587 0.78% 144 277269 0.84% 145 324631 0.98% 146 346288 1.05% 147 426567 1.29% 148 583445 1.77% 149 1040845 3.16% 150 5759941 17.47% 151 18543129 56.23% 32976720 reads passed initial QC criterion=sequence-density sequence-density=1.20 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=36 prefix-density=1.18 prefix-fanout=2.0 sequence=CCACTACGCAACTTGGAACGGGCGGGCCATCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=1403.44 fanout-score-rank=1 prefix-density=9.67 prefix-fanout=1.0 sequence=TACTTCCATAAAATTATTTTACTCTTTTAGTTGGAACCTTAGGCGGTTCTCGGAAGAAGATAGCGAAAAAAATTATCCCTAAAGTCGAAACTAAAAGGAACGTATAAACCAATGCTTCCATAGATTCGATCGTGGTTTATTTACAATTATAACTTCCACACCTATTCATTTTTCATTTGGGAAAATTTCCCATATAAAG criterion=sequence-density sequence-density=1.34 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=33 prefix-density=1.36 prefix-fanout=2.0 sequence=GCGTGAGGCTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=180.89 fanout-score-rank=1 prefix-density=0.52 prefix-fanout=3.8 sequence=AAAGGAAAATGGGGATATGGCGAAATCGGTAGACGCTACGGACTTGATTGTATTGAGCCTTAGTATGGAAACCTGCTAAGTGTTAACTTCCAAATTCAGAGAAACCCTGGAATTAAAAAAGGGCAATCCTGAGCCAAATCCGTGTTTTGAGAAAACAAGGGGTTCTCGAACTAGAATCCAAAGGAAAAGGATAGGTGCAGAGACTCAATGGAAGCTGTTCTAACGAATCGAGTTAATTTATTTAGGTTGTTTTGG SRR8846559 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 03:22:50 Started mapping on | Dec 12 03:22:51 Finished on | Dec 12 03:50:12 Mapping speed, Million of reads per hour | 72.34 Number of input reads | 32976720 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 19265752 Uniquely mapped reads % | 58.42% Average mapped length | 292.91 Number of splices: Total | 3778622 Number of splices: Annotated (sjdb) | 3374914 Number of splices: GT/AG | 3633458 Number of splices: GC/AG | 42257 Number of splices: AT/AC | 12625 Number of splices: Non-canonical | 90282 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 1.76 Insertion rate per base | 0.02% Insertion average length | 1.75 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12449126 % of reads mapped to multiple loci | 37.75% Number of reads mapped to too many loci | 3449 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.47% % of reads unmapped: other | 0.35% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1269315 1269315 1269315 N_multimapping 12449126 12449126 12449126 N_noFeature 5063575 18273336 5504719 N_ambiguous 1132373 39659 589398 UnstrandedReadsAssigned:13069804 PositiveStrandReadsAssigned:952757 NegativeStrandReadsAssigned:13171635 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=147 echo kmer=143 SRR8846559 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR8846559-trimmed-pair1.fastq SRR8846559-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,976,720 reads, 18,870,085 reads pseudoaligned [quant] estimated average fragment length: 217.8 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,061 rounds 52973 SRR8846559.ke.tsv 35125 SRR8846559.se.tsv 88098 total ==> SRR8846559.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 719.776 0 0 PNS24247 1044 827.2 6.60143 0.455687 PNS24249 1928 1711.2 12.6054 0.420626 PNS24246 1044 827.2 6.60143 0.455687 PNS24248 1044 827.2 6.60143 0.455687 PNS24244 1471 1254.2 39.5903 1.80244 PNS24243 293 109.045 0 0 KQK14069 1603 1386.2 1878.13 77.3643 KQK14071 474 265.334 22.2119 4.78003 ==> SRR8846559.se.tsv <== BRADI_1g14170v3 2242 BRADI_1g53295v3 14 BRADI_1g59795v3 34 BRADI_1g07683v3 0 BRADI_1g00485v3 12 BRADI_1g20270v3 303 BRADI_1g74790v3 20 BRADI_1g09890v3 0 BRADI_1g77505v3 46 BRADI_1g48960v3 0 SRR8846559 completed mapping pipeline successfully