Starting /dee2/code/volunteer_pipeline.sh SRR10225145 current disk space = 3052063145984 free memory = 1230757872 SRR10225145 SRAfilesize 1b6c4561c64fe615ebe22fb5983340f5 SRR10225145.sra SRR10225145.sra file validated SRR10225145 is paired end SRR10225145 is conventional basespace SRR10225145 read1 length is 100 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR10225145_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86125 34.0 33.0 34.0 31.0 34.0 2 33.0675 34.0 33.0 34.0 31.0 34.0 3 33.1625 34.0 33.0 34.0 31.0 34.0 4 36.47425 37.0 37.0 37.0 35.0 37.0 5 36.4025 37.0 37.0 37.0 35.0 37.0 6 36.385 37.0 37.0 37.0 35.0 37.0 7 36.4435 37.0 37.0 37.0 35.0 37.0 8 36.3945 37.0 37.0 37.0 35.0 37.0 9 38.281 39.0 39.0 39.0 37.0 39.0 10-11 38.19125 39.0 39.0 39.0 37.0 39.0 12-13 38.246625 39.0 39.0 39.0 37.0 39.0 14-15 39.824875 41.0 40.0 41.0 37.5 41.0 16-17 39.719625 41.0 40.0 41.0 37.0 41.0 18-19 39.696124999999995 41.0 40.0 41.0 37.0 41.0 20-21 39.692 41.0 40.0 41.0 37.0 41.0 22-23 39.640375 41.0 40.0 41.0 37.0 41.0 24-25 39.566374999999994 41.0 40.0 41.0 36.5 41.0 26-27 39.507000000000005 41.0 40.0 41.0 36.5 41.0 28-29 39.35787500000001 41.0 39.0 41.0 36.0 41.0 30-31 39.23075 41.0 39.0 41.0 36.0 41.0 32-33 39.057375 41.0 39.0 41.0 35.5 41.0 34-35 38.9505 40.0 39.0 41.0 35.0 41.0 36-37 38.76675 40.0 38.0 41.0 35.0 41.0 38-39 38.701 40.0 38.0 41.0 35.0 41.0 40-41 38.417 40.0 38.0 41.0 34.0 41.0 42-43 38.219 40.0 38.0 41.0 34.0 41.0 44-45 38.487875 40.0 38.0 41.0 34.0 41.0 46-47 38.476625 40.0 38.0 41.0 34.0 41.0 48-49 38.445750000000004 40.0 38.0 41.0 34.0 41.0 50-51 38.257375 40.0 37.5 41.0 34.0 41.0 52-53 38.090625 40.0 37.0 41.0 33.5 41.0 54-55 37.83075 40.0 37.0 41.0 33.0 41.0 56-57 37.608875 40.0 36.0 41.0 33.0 41.0 58-59 37.215625 39.0 35.5 41.0 33.0 41.0 60-61 36.914500000000004 39.0 35.0 41.0 32.0 41.0 62-63 36.6605 39.0 35.0 40.5 32.0 41.0 64-65 36.351875 37.5 35.0 40.0 31.5 41.0 66-67 36.013 37.0 35.0 39.5 31.5 41.0 68-69 35.66525 36.5 35.0 39.0 31.0 41.0 70-71 35.35225 36.0 35.0 39.0 31.5 41.0 72-73 34.963375 35.5 35.0 38.0 31.0 40.0 74-75 34.201 35.0 35.0 37.0 30.5 39.0 76-77 33.814625 35.0 34.5 37.0 30.0 39.0 78-79 33.605374999999995 35.0 34.0 36.0 30.0 38.0 80-81 33.319500000000005 35.0 34.0 36.0 29.5 37.0 82-83 33.095625 35.0 34.0 35.5 29.5 37.0 84-85 32.98125 35.0 34.0 35.0 30.0 36.0 86-87 32.761125 35.0 34.0 35.0 29.5 36.0 88-89 32.554500000000004 35.0 34.0 35.0 29.0 36.0 90-91 32.42425 35.0 34.0 35.0 29.0 36.0 92-93 32.355625 35.0 34.0 35.0 29.0 35.5 94-95 32.250625 35.0 34.0 35.0 29.0 35.0 96-97 32.10925 35.0 34.0 35.0 28.5 35.0 98-99 32.012875 35.0 34.0 35.0 28.0 35.0 100 31.90475 35.0 34.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-11 0.0 1101 12-13 0.0 1101 14-15 0.0 1101 16-17 0.0 1101 18-19 0.0 1101 20-21 0.0 1101 22-23 0.0 1101 24-25 0.0 1101 26-27 0.0 1101 28-29 0.0 1101 30-31 0.0 1101 32-33 0.0 1101 34-35 0.0 1101 36-37 0.0 1101 38-39 0.0 1101 40-41 0.0 1101 42-43 0.0 1101 44-45 0.0 1101 46-47 0.0 1101 48-49 0.0 1101 50-51 0.0 1101 52-53 0.0 1101 54-55 0.0 1101 56-57 0.0 1101 58-59 0.0 1101 60-61 0.0 1101 62-63 0.0 1101 64-65 0.0 1101 66-67 0.0 1101 68-69 0.0 1101 70-71 0.0 1101 72-73 0.0 1101 74-75 0.0 1101 76-77 0.0 1101 78-79 0.0 1101 80-81 0.0 1101 82-83 0.0 1101 84-85 0.0 1101 86-87 0.0 1101 88-89 0.0 1101 90-91 0.0 1101 92-93 0.0 1101 94-95 0.0 1101 96-97 0.0 1101 98-99 0.0 1101 100 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 1.0 12 3.0 13 4.0 14 3.0 15 5.0 16 6.0 17 6.0 18 13.0 19 9.0 20 10.0 21 4.0 22 7.0 23 10.0 24 21.0 25 19.0 26 14.0 27 32.0 28 37.0 29 66.0 30 46.0 31 63.0 32 73.0 33 93.0 34 130.0 35 179.0 36 430.0 37 995.0 38 1426.0 39 291.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.293319939728782 39.55298844801607 21.72275238573581 12.430939226519337 2 32.25 32.975 18.925 15.85 3 30.65 33.125 21.9 14.325 4 28.325 31.324999999999996 23.599999999999998 16.75 5 25.525 32.2 24.375 17.9 6 31.624999999999996 26.55 22.225 19.6 7 29.75 28.425 23.3 18.525 8 23.925 31.275 28.1 16.7 9 23.724999999999998 32.775 26.275 17.224999999999998 10-11 25.362499999999997 31.8 24.725 18.1125 12-13 22.85 31.724999999999998 25.887500000000003 19.537499999999998 14-15 23.799999999999997 30.15 26.174999999999997 19.875 16-17 23.724999999999998 29.512500000000003 26.075 20.6875 18-19 23.4625 29.1375 27.625 19.775000000000002 20-21 24.2875 29.9 27.2625 18.55 22-23 24.8125 30.412499999999998 25.362499999999997 19.412499999999998 24-25 22.8625 30.1875 25.724999999999998 21.224999999999998 26-27 24.0 28.8875 26.825 20.2875 28-29 22.75 30.475 26.2875 20.4875 30-31 23.799999999999997 29.1875 27.5875 19.425 32-33 23.7875 29.9625 26.275 19.975 34-35 24.025 29.062500000000004 26.4125 20.5 36-37 23.6875 28.9875 26.275 21.05 38-39 23.575 30.9625 26.3625 19.1 40-41 22.9625 29.875 27.8375 19.325 42-43 24.175 29.6375 25.85 20.3375 44-45 22.900000000000002 28.7 27.3875 21.0125 46-47 23.799999999999997 29.525000000000002 26.8 19.875 48-49 23.375 29.0875 27.725 19.8125 50-51 24.45 29.325000000000003 26.275 19.950000000000003 52-53 25.1875 29.15 24.875 20.7875 54-55 24.2875 28.525 27.250000000000004 19.9375 56-57 23.6375 28.8625 28.000000000000004 19.5 58-59 23.4625 28.075 28.325 20.1375 60-61 24.099999999999998 28.4 26.974999999999998 20.525 62-63 23.1625 28.525 29.037499999999998 19.275000000000002 64-65 24.6875 30.725 25.887500000000003 18.7 66-67 23.65 30.025000000000002 27.4125 18.912499999999998 68-69 23.400000000000002 29.775000000000002 27.474999999999998 19.35 70-71 23.052881610201275 30.428803600450056 27.353419177397175 19.164895611951493 72-73 24.2375 30.2625 26.737499999999997 18.7625 74-75 23.9375 30.362499999999997 26.325 19.375 76-77 25.0375 28.775000000000002 26.887499999999996 19.3 78-79 23.8625 29.1375 27.4125 19.5875 80-81 24.962500000000002 29.15 26.325 19.5625 82-83 23.075000000000003 28.599999999999998 27.437499999999996 20.8875 84-85 23.3625 29.549999999999997 27.187499999999996 19.900000000000002 86-87 25.362499999999997 28.249999999999996 26.987499999999997 19.400000000000002 88-89 24.65 27.787499999999998 26.474999999999998 21.087500000000002 90-91 24.5375 29.262500000000003 26.474999999999998 19.725 92-93 23.962500000000002 27.987499999999997 27.725 20.325 94-95 23.4875 28.425 28.225 19.8625 96-97 23.9 28.199999999999996 27.8625 20.0375 98-99 24.0375 28.475 28.3375 19.15 100 25.650000000000002 27.125 27.425 19.8 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.5 6 1.0 7 0.5 8 0.0 9 0.0 10 0.5 11 1.5 12 3.0 13 3.0 14 1.5 15 2.5 16 5.5 17 4.5 18 2.0 19 5.0 20 8.0 21 6.0 22 6.5 23 11.0 24 12.5 25 11.5 26 9.5 27 13.0 28 17.0 29 19.0 30 24.5 31 30.5 32 39.5 33 43.0 34 55.0 35 83.0 36 104.5 37 127.0 38 127.5 39 145.5 40 194.0 41 219.0 42 237.5 43 240.0 44 224.0 45 232.0 46 246.0 47 218.0 48 195.0 49 181.0 50 150.5 51 134.0 52 108.0 53 85.5 54 79.5 55 60.0 56 43.5 57 35.0 58 26.5 59 22.5 60 23.0 61 18.5 62 16.0 63 12.5 64 9.5 65 10.0 66 7.0 67 6.0 68 9.0 69 8.5 70 4.5 71 4.5 72 5.0 73 3.5 74 2.0 75 1.5 76 0.5 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44999999999999996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0125 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.925 #Duplication Level Percentage of deduplicated Percentage of total 1 98.1301027126679 93.15 2 1.2641559125625494 2.4 3 0.23702923360547798 0.675 4 0.07900974453515934 0.3 5 0.18435607058203846 0.8750000000000001 6 0.0 0.0 7 0.02633658151171978 0.17500000000000002 8 0.02633658151171978 0.2 9 0.0 0.0 >10 0.02633658151171978 0.675 >50 0.02633658151171978 1.55 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG 62 1.55 TruSeq Adapter, Index 6 (100% over 49bp) CAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTG 27 0.675 No Hit TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC 8 0.2 No Hit GCTCTTAGTACTGCACCATCTCATCGTCATGTGATCCTTTTGCTCCTCCC 7 0.17500000000000002 No Hit AGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAA 5 0.125 No Hit AACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCT 5 0.125 No Hit GCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAA 5 0.125 No Hit GGGAGAAAAATTCCTCTAAAAAACAAAATAAAAATTAACAAAAAGTCAAA 5 0.125 No Hit GGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTG 5 0.125 No Hit GGTGTTGCGGGGAATTTGGACTGTTACTTTGAAAAAATTAGAGTGTTTAA 5 0.125 No Hit TTTGACCTCAAATCAGGTAGGAACACCCGCTGAACTTAAGCATATCAATA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.075 0.0 0.0 0.0 0.0 2 2.125 0.0 0.0 0.0 0.0 3 2.125 0.0 0.0 0.0 0.0 4 2.125 0.0 0.0 0.0 0.0 5 2.125 0.0 0.0 0.0 0.0 6 2.125 0.0 0.0 0.0 0.0 7 2.125 0.0 0.0 0.0 0.0 8 2.125 0.0 0.0 0.0 0.0 9 2.125 0.0 0.0 0.0 0.0 10-11 2.125 0.0 0.0 0.0 0.0 12-13 2.125 0.0 0.0 0.0 0.0 14-15 2.1375 0.0 0.0 0.0 0.0 16-17 2.15 0.0 0.0 0.0 0.0 18-19 2.15 0.0 0.0 0.0 0.0 20-21 2.15 0.0 0.0 0.0 0.0 22-23 2.15 0.0 0.0 0.0 0.0 24-25 2.15 0.0 0.0 0.0 0.0 26-27 2.15 0.0 0.0 0.0 0.0 28-29 2.175 0.0 0.0 0.0 0.0 30-31 2.1875 0.0 0.0 0.0 0.0 32-33 2.2 0.0 0.0 0.0 0.0 34-35 2.2 0.0 0.0 0.0 0.0 36-37 2.2 0.0 0.0 0.0 0.0 38-39 2.2 0.0 0.0 0.0 0.0 40-41 2.2 0.0 0.0 0.0 0.0 42-43 2.2 0.0 0.0 0.0 0.0 44-45 2.2 0.0 0.0 0.0 0.0 46-47 2.2 0.0 0.0 0.0 0.0 48-49 2.2 0.0 0.0 0.0 0.0 50-51 2.2 0.0 0.0 0.0 0.0 52-53 2.2 0.0 0.0 0.0 0.0 54-55 2.225 0.0 0.0 0.0 0.0 56-57 2.2375 0.0 0.0 0.0 0.0 58-59 2.25 0.0 0.0 0.0 0.0 60-61 2.2874999999999996 0.0 0.0 0.0 0.0 62-63 2.3 0.0 0.0 0.0 0.0 64-65 2.3125 0.0 0.0 0.0 0.0 66-67 2.35 0.0 0.0 0.0 0.0 68-69 2.35 0.0 0.0 0.0 0.0 70-71 2.35 0.0 0.0 0.0 0.0 72-73 2.3875 0.0 0.0 0.0 0.0 74-75 2.4 0.0 0.0 0.0 0.0 76-77 2.4 0.0 0.0 0.0 0.0 78-79 2.4 0.0 0.0 0.0 0.0 80-81 2.4 0.0 0.0 0.0 0.0 82-83 2.4 0.0 0.0 0.0 0.0 84-85 2.4124999999999996 0.0 0.0 0.0 0.0 86-87 2.425 0.0 0.0 0.0 0.0 88 2.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 30 9.30595E-9 95.17722 1 TCGGAAG 35 2.5283953E-10 93.9875 4 CGGAAGA 35 2.5283953E-10 93.9875 5 ATCGGAA 35 2.5283953E-10 93.9875 3 AAGAGCA 40 7.2941475E-10 82.23906 8 GATCGGA 40 7.2941475E-10 82.23906 2 GAAGAGC 40 7.2941475E-10 82.23906 7 AGAGCAC 40 7.2941475E-10 82.23906 9 GGAAGAG 45 1.8535502E-9 73.101395 6 ACACGTC 35 7.270137E-8 46.99375 14-15 GTATGCC 35 7.270137E-8 46.99375 46-47 CCAATAT 35 7.270137E-8 46.99375 36-37 GTCACGC 35 7.270137E-8 46.99375 30-31 CACACGT 35 7.270137E-8 46.99375 12-13 ACGTCTG 35 7.270137E-8 46.99375 16-17 TGCCGTC 35 7.270137E-8 46.99375 48-49 CCAGTCA 30 1.4145098E-6 46.99375 26-27 CACGCCA 35 7.270137E-8 46.99375 32-33 CACGTCT 35 7.270137E-8 46.99375 14-15 TATGCCG 35 7.270137E-8 46.99375 46-47 >>END_MODULE SRR10225145 read2 length is 100 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR10225145_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31475 34.0 31.0 34.0 31.0 34.0 2 32.50725 34.0 31.0 34.0 31.0 34.0 3 32.50225 34.0 31.0 34.0 31.0 34.0 4 35.98575 37.0 37.0 37.0 35.0 37.0 5 35.84525 37.0 37.0 37.0 35.0 37.0 6 35.78675 37.0 37.0 37.0 35.0 37.0 7 35.75 37.0 37.0 37.0 35.0 37.0 8 35.8015 37.0 37.0 37.0 35.0 37.0 9 37.6325 39.0 39.0 39.0 35.0 39.0 10-11 37.6045 39.0 39.0 39.0 35.0 39.0 12-13 37.55175 39.0 39.0 39.0 35.0 39.0 14-15 39.081875 41.0 40.0 41.0 36.0 41.0 16-17 39.059124999999995 41.0 40.0 41.0 36.0 41.0 18-19 38.870374999999996 41.0 39.0 41.0 35.0 41.0 20-21 38.760625000000005 41.0 39.0 41.0 35.5 41.0 22-23 38.624875 41.0 39.0 41.0 35.0 41.0 24-25 38.503125 41.0 39.0 41.0 34.5 41.0 26-27 38.341625 41.0 39.0 41.0 34.0 41.0 28-29 38.21725 41.0 39.0 41.0 34.0 41.0 30-31 37.936375 40.0 38.0 41.0 33.5 41.0 32-33 37.806375 40.0 38.0 41.0 33.0 41.0 34-35 37.694125 40.0 38.0 41.0 33.0 41.0 36-37 37.620625000000004 40.0 38.0 41.0 32.5 41.0 38-39 37.4815 40.0 38.0 41.0 32.5 41.0 40-41 37.563874999999996 40.0 38.0 41.0 32.5 41.0 42-43 37.493375 40.0 38.0 41.0 33.0 41.0 44-45 37.512 40.0 38.0 41.0 32.5 41.0 46-47 37.619375000000005 40.0 38.0 41.0 33.0 41.0 48-49 37.53975 40.0 38.0 41.0 32.5 41.0 50-51 37.4285 40.0 37.5 41.0 33.0 41.0 52-53 37.203374999999994 40.0 37.0 41.0 31.5 41.0 54-55 37.071875 40.0 37.0 41.0 32.0 41.0 56-57 36.86925 40.0 36.0 41.0 31.5 41.0 58-59 36.667375 40.0 36.0 41.0 31.0 41.0 60-61 36.374875 39.0 35.0 41.0 31.0 41.0 62-63 36.08825 39.0 35.0 41.0 31.0 41.0 64-65 35.583625 38.0 35.0 40.5 30.0 41.0 66-67 34.9305 37.0 35.0 40.0 28.0 41.0 68-69 34.482124999999996 37.0 35.0 39.0 28.0 41.0 70-71 34.1935 36.0 35.0 39.0 28.5 41.0 72-73 33.780249999999995 36.0 34.5 39.0 27.5 40.0 74-75 33.380625 35.0 34.0 37.0 26.5 39.5 76-77 33.14375 35.0 34.0 37.0 27.5 39.0 78-79 32.78675 35.0 34.0 36.5 27.0 39.0 80-81 32.512625 35.0 34.0 36.0 26.0 37.0 82-83 32.1575 35.0 34.0 36.0 25.0 37.0 84-85 31.894875 35.0 34.0 35.5 25.0 36.5 86-87 31.69025 35.0 33.5 35.0 25.0 36.0 88-89 31.545125 35.0 33.5 35.0 24.5 36.0 90-91 31.296750000000003 35.0 33.0 35.0 23.0 36.0 92-93 31.19525 35.0 33.0 35.0 22.0 35.5 94-95 30.948999999999998 35.0 33.0 35.0 20.0 35.0 96-97 30.746375 35.0 33.0 35.0 18.0 35.0 98-99 30.639875 35.0 33.0 35.0 8.5 35.0 100 30.53 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-11 0.0 1101 12-13 0.0 1101 14-15 0.0 1101 16-17 0.0 1101 18-19 0.0 1101 20-21 0.0 1101 22-23 0.0 1101 24-25 0.0 1101 26-27 0.0 1101 28-29 0.0 1101 30-31 0.0 1101 32-33 0.0 1101 34-35 0.0 1101 36-37 0.0 1101 38-39 0.0 1101 40-41 0.0 1101 42-43 0.0 1101 44-45 0.0 1101 46-47 0.0 1101 48-49 0.0 1101 50-51 0.0 1101 52-53 0.0 1101 54-55 0.0 1101 56-57 0.0 1101 58-59 0.0 1101 60-61 0.0 1101 62-63 0.0 1101 64-65 0.0 1101 66-67 0.0 1101 68-69 0.0 1101 70-71 0.0 1101 72-73 0.0 1101 74-75 0.0 1101 76-77 0.0 1101 78-79 0.0 1101 80-81 0.0 1101 82-83 0.0 1101 84-85 0.0 1101 86-87 0.0 1101 88-89 0.0 1101 90-91 0.0 1101 92-93 0.0 1101 94-95 0.0 1101 96-97 0.0 1101 98-99 0.0 1101 100 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29.0 3 10.0 4 8.0 5 6.0 6 4.0 7 11.0 8 6.0 9 9.0 10 13.0 11 3.0 12 6.0 13 8.0 14 5.0 15 3.0 16 6.0 17 8.0 18 9.0 19 13.0 20 16.0 21 14.0 22 18.0 23 19.0 24 26.0 25 38.0 26 35.0 27 32.0 28 34.0 29 37.0 30 49.0 31 58.0 32 51.0 33 85.0 34 131.0 35 193.0 36 374.0 37 835.0 38 1456.0 39 341.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.15 16.025 15.4 30.425 2 28.525 7.225 20.325 43.925 3 18.3 8.9 21.75 51.05 4 20.75 7.249999999999999 22.55 49.45 5 23.25 11.175 22.825 42.75 6 30.282570642660666 12.878219554888723 26.65666416604151 30.182545636409102 7 19.85 25.525 34.5 20.125 8 13.3 32.1 35.475 19.125 9 15.975 31.900000000000002 31.900000000000002 20.225 10-11 17.05 32.0 31.087500000000002 19.8625 12-13 16.6875 29.262500000000003 31.674999999999997 22.375 14-15 16.425 27.8875 33.775 21.912499999999998 16-17 17.6375 28.475 31.624999999999996 22.2625 18-19 18.025 29.4 31.5375 21.0375 20-21 17.7375 30.012499999999996 30.599999999999998 21.65 22-23 19.7 29.3875 29.512500000000003 21.4 24-25 18.75 30.862499999999997 28.95 21.4375 26-27 17.8625 31.837500000000002 29.75 20.549999999999997 28-29 19.6875 31.15 28.025 21.1375 30-31 19.037499999999998 30.062499999999996 28.5875 22.3125 32-33 18.575 28.812500000000004 29.349999999999998 23.2625 34-35 18.475 29.95 28.775000000000002 22.8 36-37 18.6125 30.025000000000002 29.299999999999997 22.0625 38-39 18.6875 28.8375 29.7125 22.7625 40-41 18.8375 30.1875 28.237499999999997 22.7375 42-43 18.925 29.799999999999997 28.5625 22.7125 44-45 19.85 30.049999999999997 27.775 22.325 46-47 18.712500000000002 28.7 28.8625 23.724999999999998 48-49 20.375 28.262500000000003 28.762500000000003 22.6 50-51 20.0125 29.275000000000002 27.150000000000002 23.5625 52-53 17.3625 31.7375 28.575 22.325 54-55 18.637500000000003 30.599999999999998 28.8375 21.925 56-57 17.712500000000002 30.9875 29.875 21.425 58-59 17.775 30.5125 29.762499999999996 21.95 60-61 18.05 31.424999999999997 27.6375 22.8875 62-63 18.275 30.662499999999998 27.6125 23.45 64-65 18.8125 29.5 27.775 23.9125 66-67 18.75 29.65 28.1375 23.4625 68-69 17.9 30.162499999999998 28.712500000000002 23.225 70-71 18.512500000000003 30.55 28.3125 22.625 72-73 19.875 29.4375 28.575 22.112499999999997 74-75 18.875 29.475 28.212500000000002 23.4375 76-77 19.825 28.762500000000003 28.712500000000002 22.7 78-79 19.425 29.862499999999997 27.55 23.1625 80-81 18.725 29.625 27.762500000000003 23.8875 82-83 19.6125 28.925 27.5625 23.9 84-85 19.075 28.262500000000003 28.3375 24.325 86-87 18.875 30.412499999999998 26.787499999999998 23.925 88-89 19.8 28.712500000000002 27.474999999999998 24.0125 90-91 19.7625 29.049999999999997 27.737499999999997 23.45 92-93 20.525 28.799999999999997 26.875 23.799999999999997 94-95 19.537499999999998 29.725 28.3875 22.35 96-97 19.5125 29.362500000000004 28.525 22.6 98-99 18.2625 29.375 29.037499999999998 23.325000000000003 100 19.275000000000002 30.45 28.075 22.2 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.5 12 0.5 13 1.5 14 4.5 15 5.5 16 4.0 17 3.0 18 4.0 19 6.5 20 9.5 21 10.0 22 10.0 23 10.0 24 15.0 25 23.5 26 25.5 27 24.0 28 26.5 29 35.0 30 47.5 31 61.5 32 75.0 33 77.0 34 77.0 35 102.0 36 140.0 37 150.0 38 161.5 39 185.5 40 201.0 41 192.5 42 179.0 43 199.5 44 211.0 45 200.0 46 188.5 47 177.5 48 162.5 49 141.5 50 119.5 51 108.5 52 85.5 53 67.0 54 62.0 55 47.5 56 38.5 57 42.5 58 48.0 59 48.5 60 38.5 61 26.5 62 23.0 63 15.0 64 12.5 65 12.5 66 8.0 67 5.5 68 4.5 69 5.0 70 3.5 71 3.5 72 5.5 73 8.5 74 6.5 75 1.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.025 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.15 #Duplication Level Percentage of deduplicated Percentage of total 1 96.67203435319377 90.05 2 2.0128824476650564 3.75 3 0.7514761137949544 2.1 4 0.2683843263553409 1.0 5 0.1878690284487386 0.8750000000000001 6 0.026838432635534086 0.15 7 0.0 0.0 8 0.05367686527106817 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.026838432635534086 1.675 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 67 1.675 Illumina Single End PCR Primer 1 (100% over 50bp) CCCTTTCAACAATTTCACGTACTGTTTAACTCTCTTTTCAAAGTTCTTTT 8 0.2 No Hit GCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCC 8 0.2 No Hit GCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTT 6 0.15 No Hit TCCCTTTCAACAATTTCACGTACTGTTTAACTCTCTTTTCAAAGTTCTTT 5 0.125 No Hit GCCCCCAACTATCCCTATTAATCATTACGTCAATCCTAGAAACCAACAAA 5 0.125 No Hit CCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTC 5 0.125 No Hit GACGCGTACACAAGACAAAGGATTTATAGGAACCCTTTGCTGTTTATTAT 5 0.125 No Hit ACCCCGTATTGTTATTTATTGTCACTACCTCCCCGTGTCGGGATTGGGTA 5 0.125 No Hit GTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGA 5 0.125 No Hit CCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.875 0.0 0.0 0.0 0.0 2 1.9 0.0 0.0 0.0 0.0 3 1.9 0.0 0.0 0.0 0.0 4 1.9 0.0 0.0 0.0 0.0 5 1.9 0.0 0.0 0.0 0.0 6 1.9 0.0 0.0 0.0 0.0 7 1.9 0.0 0.0 0.0 0.0 8 1.9 0.0 0.0 0.0 0.0 9 1.9 0.0 0.0 0.0 0.0 10-11 1.9 0.0 0.0 0.0 0.0 12-13 1.9 0.0 0.0 0.0 0.0 14-15 1.9249999999999998 0.0 0.0 0.0 0.0 16-17 1.95 0.0 0.0 0.0 0.0 18-19 1.9625 0.0 0.0 0.0 0.0 20-21 2.0 0.0 0.0 0.0 0.0 22-23 2.0374999999999996 0.0 0.0 0.0 0.0 24-25 2.05 0.0 0.0 0.0 0.0 26-27 2.0625 0.0 0.0 0.0 0.0 28-29 2.1 0.0 0.0 0.0 0.0 30-31 2.1125 0.0 0.0 0.0 0.0 32-33 2.125 0.0 0.0 0.0 0.0 34-35 2.15 0.0 0.0 0.0 0.0 36-37 2.15 0.0 0.0 0.0 0.0 38-39 2.15 0.0 0.0 0.0 0.0 40-41 2.15 0.0 0.0 0.0 0.0 42-43 2.15 0.0 0.0 0.0 0.0 44-45 2.15 0.0 0.0 0.0 0.0 46-47 2.15 0.0 0.0 0.0 0.0 48-49 2.15 0.0 0.0 0.0 0.0 50-51 2.15 0.0 0.0 0.0 0.0 52-53 2.15 0.0 0.0 0.0 0.0 54-55 2.175 0.0 0.0 0.0 0.0 56-57 2.1875 0.0 0.0 0.0 0.0 58-59 2.2 0.0 0.0 0.0 0.0 60-61 2.2375 0.0 0.0 0.0 0.0 62-63 2.25 0.0 0.0 0.0 0.0 64-65 2.2625 0.0 0.0 0.0 0.0 66-67 2.3 0.0 0.0 0.0 0.0 68-69 2.3 0.0 0.0 0.0 0.0 70-71 2.3 0.0 0.0 0.0 0.0 72-73 2.3125 0.0 0.0 0.0 0.0 74-75 2.325 0.0 0.0 0.0 0.0 76-77 2.325 0.0 0.0 0.0 0.0 78-79 2.325 0.0 0.0 0.0 0.0 80-81 2.325 0.0 0.0 0.0 0.0 82-83 2.325 0.0 0.0 0.0 0.0 84-85 2.3375000000000004 0.0 0.0 0.0 0.0 86-87 2.35 0.0 0.0 0.0 0.0 88 2.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGAGCG 35 2.5283953E-10 93.99999 8 GATCGGA 35 2.5283953E-10 93.99999 2 GAAGAGC 35 2.5283953E-10 93.99999 7 TCGGAAG 35 2.5283953E-10 93.99999 4 CGGAAGA 35 2.5283953E-10 93.99999 5 ATCGGAA 35 2.5283953E-10 93.99999 3 GGAAGAG 35 2.5283953E-10 93.99999 6 AGATCGG 35 2.5283953E-10 93.99999 1 AGAGCGT 40 7.2759576E-10 82.25 9 GTAGATC 35 7.262497E-8 46.999996 32-33 GAGCGTC 35 7.262497E-8 46.999996 10-11 TGGTCGC 35 7.262497E-8 46.999996 42-43 ATCTCGG 35 7.262497E-8 46.999996 36-37 GAGTGTA 35 7.262497E-8 46.999996 28-29 GTCGTGT 35 7.262497E-8 46.999996 14-15 GTCGCCG 35 7.262497E-8 46.999996 44-45 GTGTAGG 35 7.262497E-8 46.999996 16-17 GTGTAGA 35 7.262497E-8 46.999996 30-31 AAGAGTG 35 7.262497E-8 46.999996 26-27 GTATCAT 35 7.262497E-8 46.999996 50-51 >>END_MODULE Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011125 spots for SRR10225145.sra Written 5011125 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra Read 5011108 spots for SRR10225145.sra Written 5011108 spots for SRR10225145.sra SRR ids: ['SRR10225145.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3q7nhg15 SRR10225145.sra spots: 100222177 blocks: [[1, 5011108], [5011109, 10022216], [10022217, 15033324], [15033325, 20044432], [20044433, 25055540], [25055541, 30066648], [30066649, 35077756], [35077757, 40088864], [40088865, 45099972], [45099973, 50111080], [50111081, 55122188], [55122189, 60133296], [60133297, 65144404], [65144405, 70155512], [70155513, 75166620], [75166621, 80177728], [80177729, 85188836], [85188837, 90199944], [90199945, 95211052], [95211053, 100222177]] SRR10225145 file size 27408755 SRR10225145 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR10225145 SRR10225145_1.fastq SRR10225145_2.fastq Input file: SRR10225145_1.fastq Paired file: SRR10225145_2.fastq trimmed: SRR10225145-trimmed-pair1.fastq, SRR10225145-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 23:59:08 2025 >> started Wed Feb 12 00:00:53 2025 >> done (104.445s) 100222177 read pairs processed; of these: 634668 ( 0.63%) short read pairs filtered out after trimming by size control 3363376 ( 3.36%) empty read pairs filtered out after trimming by size control 96224133 (96.01%) read pairs available; of these: 13832814 (14.38%) trimmed read pairs available after processing 82391319 (85.62%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 26257 0.03% 19 17459 0.02% 20 22637 0.02% 21 10142 0.01% 22 6625 0.01% 23 8105 0.01% 24 18336 0.02% 25 19899 0.02% 26 15715 0.02% 27 11961 0.01% 28 8624 0.01% 29 10500 0.01% 30 10777 0.01% 31 8055 0.01% 32 8494 0.01% 33 6649 0.01% 34 5480 0.01% 35 5963 0.01% 36 6402 0.01% 37 6898 0.01% 38 7594 0.01% 39 8080 0.01% 40 8944 0.01% 41 9431 0.01% 42 9835 0.01% 43 11534 0.01% 44 11525 0.01% 45 12561 0.01% 46 12976 0.01% 47 13671 0.01% 48 14962 0.02% 49 15982 0.02% 50 17215 0.02% 51 18494 0.02% 52 19177 0.02% 53 20742 0.02% 54 22611 0.02% 55 26090 0.03% 56 26353 0.03% 57 28894 0.03% 58 30354 0.03% 59 159372 0.17% 60 169520 0.18% 61 108527 0.11% 62 120084 0.12% 63 130468 0.14% 64 129911 0.14% 65 136425 0.14% 66 143411 0.15% 67 147584 0.15% 68 144617 0.15% 69 149520 0.16% 70 151903 0.16% 71 149494 0.16% 72 150371 0.16% 73 160879 0.17% 74 161119 0.17% 75 167322 0.17% 76 178846 0.19% 77 169341 0.18% 78 169704 0.18% 79 173935 0.18% 80 183593 0.19% 81 193865 0.20% 82 202501 0.21% 83 217797 0.23% 84 223779 0.23% 85 226177 0.24% 86 218467 0.23% 87 240745 0.25% 88 256966 0.27% 89 286168 0.30% 90 335181 0.35% 91 502247 0.52% 92 351958 0.37% 93 420147 0.44% 94 431115 0.45% 95 1777924 1.85% 96 577482 0.60% 97 717903 0.75% 98 1014393 1.05% 99 1700050 1.77% 100 82391319 85.62% 96224133 reads passed initial QC criterion=sequence-density sequence-density=1.71 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=26 prefix-density=1.69 prefix-fanout=1.9 sequence=GATTCCCCTAGTAAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=33.03 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=3.2 sequence=AGAAATCAAAGTTTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTCTATAGCCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTC criterion=sequence-density sequence-density=1.02 sequence-density-rank=1 fanout-score=2.60 fanout-score-rank=21 prefix-density=2.64 prefix-fanout=1.0 sequence=GGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=105.58 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=11.7 sequence=TTTCCTCTTTAAGTTTGGCAGCCTCTTTACTTATTTCCTGGAATTTTTCCCTTACTTCCATTTGACCCTCCCTTATGCGTTTTTGACCCTCGCTAATCTCAACCATGTCAGCTCTTATCTTTTTGATGCTCCTACTTAATTTTTTGTCACTTTTTTTCCGTTTCAGATTCTTCCTTTGAAGCGCTCCGATGGAGGACCTCTGCACAACTCGTTTTGTATCCATGTTGCTCCTCATCTTAGGCGCAGGTAAAATGAT SRR10225145 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:01:34 Started mapping on | Feb 12 00:01:34 Finished on | Feb 12 00:21:43 Mapping speed, Million of reads per hour | 286.52 Number of input reads | 96224133 Average input read length | 195 UNIQUE READS: Uniquely mapped reads number | 65244832 Uniquely mapped reads % | 67.81% Average mapped length | 194.43 Number of splices: Total | 22010838 Number of splices: Annotated (sjdb) | 21254246 Number of splices: GT/AG | 21454843 Number of splices: GC/AG | 338721 Number of splices: AT/AC | 35480 Number of splices: Non-canonical | 181794 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.05% Deletion average length | 2.35 Insertion rate per base | 0.04% Insertion average length | 2.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 3230988 % of reads mapped to multiple loci | 3.36% Number of reads mapped to too many loci | 8547544 % of reads mapped to too many loci | 8.88% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 18.00% % of reads unmapped: other | 1.96% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 28204823 28204823 28204823 N_multimapping 3230988 3230988 3230988 N_noFeature 4275243 5221419 63660076 N_ambiguous 1176852 527514 14955 UnstrandedReadsAssigned:59792737 PositiveStrandReadsAssigned:59495899 NegativeStrandReadsAssigned:1569801 Dataset is classified positive stranded MeadianReadLen=100 20thPercentileLength=100 echo kmer=95 SRR10225145 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR10225145-trimmed-pair1.fastq SRR10225145-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 96,224,133 reads, 66,571,761 reads pseudoaligned [quant] estimated average fragment length: 248.953 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,260 rounds 52401 SRR10225145.ke.tsv 34699 SRR10225145.se.tsv 87100 total ==> SRR10225145.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1770.05 14744 79.5253 Potri.005G024800.1.v4.1 1035 787.047 1407 17.0674 Potri.004G059700.1.v4.1 961 713.051 2588 34.6512 Potri.007G009000.2.v4.1 1416 1168.05 0 0 Potri.003G141000.2.v4.1 2943 2695.05 2202 7.80056 Potri.016G087400.1.v4.1 270 63.9025 5106.2 762.878 Potri.015G069301.1.v4.1 564 316.577 0 0 Potri.010G195200.1.v4.1 1773 1525.05 177 1.10806 Potri.012G127500.1.v4.1 977 729.047 8020 105.025 ==> SRR10225145.se.tsv <== Potri.001G166300.v4.1 1 Potri.001G448400.v4.1 25 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 1079 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 19 Potri.001G416900.v4.1 19 Potri.001G452600.v4.1 1292 SRR10225145 completed mapping pipeline successfully