Starting /dee2/code/volunteer_pipeline.sh SRR11845458 current disk space = 3088811364352 free memory = 1575148196 SRR11845458 SRAfilesize 369a597af7de41fd8b588f14e75d7f0e SRR11845458.sra SRR11845458.sra file validated SRR11845458 is paired end SRR11845458 is conventional basespace SRR11845458 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR11845458_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.042 37.0 37.0 37.0 37.0 37.0 2 35.6075 37.0 37.0 37.0 37.0 37.0 3 36.0295 37.0 37.0 37.0 37.0 37.0 4 36.1635 37.0 37.0 37.0 37.0 37.0 5 36.1355 37.0 37.0 37.0 37.0 37.0 6 36.2295 37.0 37.0 37.0 37.0 37.0 7 35.986 37.0 37.0 37.0 37.0 37.0 8 36.0695 37.0 37.0 37.0 37.0 37.0 9 36.112 37.0 37.0 37.0 37.0 37.0 10-14 36.222699999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.227900000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.1915 37.0 37.0 37.0 37.0 37.0 25-29 36.1156 37.0 37.0 37.0 37.0 37.0 30-34 36.100100000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.1029 37.0 37.0 37.0 37.0 37.0 40-44 36.0372 37.0 37.0 37.0 37.0 37.0 45-49 35.938300000000005 37.0 37.0 37.0 37.0 37.0 50-54 35.7582 37.0 37.0 37.0 37.0 37.0 55-59 35.66289999999999 37.0 37.0 37.0 37.0 37.0 60-64 35.644099999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.521699999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.6498 37.0 37.0 37.0 37.0 37.0 75-79 35.626200000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.485400000000006 37.0 37.0 37.0 34.6 37.0 85-89 35.751400000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.564 37.0 37.0 37.0 37.0 37.0 95-99 35.575900000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.611599999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.581 37.0 37.0 37.0 37.0 37.0 110-114 35.5363 37.0 37.0 37.0 37.0 37.0 115-119 35.4332 37.0 37.0 37.0 34.6 37.0 120-124 35.28750000000001 37.0 37.0 37.0 32.2 37.0 125-129 35.156400000000005 37.0 37.0 37.0 27.4 37.0 130-134 35.21040000000001 37.0 37.0 37.0 29.8 37.0 135-139 35.115899999999996 37.0 37.0 37.0 27.4 37.0 140-144 35.0609 37.0 37.0 37.0 27.4 37.0 145-149 34.6322 37.0 37.0 37.0 25.0 37.0 150 34.65 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 0.0 24 2.0 25 4.0 26 13.0 27 25.0 28 36.0 29 42.0 30 70.0 31 78.0 32 104.0 33 195.0 34 234.0 35 519.0 36 2413.0 37 264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8 4.425 4.8 48.975 2 23.879093198992443 3.9798488664987404 30.856423173803528 41.28463476070529 3 24.75 4.2 16.475 54.574999999999996 4 31.7 7.7 17.224999999999998 43.375 5 36.575 12.025 20.724999999999998 30.675 6 31.05 15.950000000000001 27.55 25.45 7 19.675 22.95 39.95 17.424999999999997 8 18.0 18.099999999999998 36.275 27.625 9 24.075 15.675 33.525 26.724999999999998 10-14 21.88 26.625 27.68 23.815 15-19 27.42 26.224999999999998 24.545 21.81 20-24 25.525 24.765 25.230000000000004 24.48 25-29 24.0 23.135 25.759999999999998 27.105 30-34 21.54 23.665 22.795 32.0 35-39 25.865 18.905 26.040000000000003 29.189999999999998 40-44 26.32 21.37 25.4 26.91 45-49 23.605 21.7 24.51 30.185000000000002 50-54 22.415 22.035 25.264999999999997 30.285 55-59 19.009999999999998 26.275 25.405 29.310000000000002 60-64 24.310000000000002 23.115 26.369999999999997 26.205000000000002 65-69 18.990000000000002 18.895 30.104999999999997 32.01 70-74 22.31 22.98 27.689999999999998 27.02 75-79 28.035 24.490000000000002 19.275000000000002 28.199999999999996 80-84 23.395 24.52 21.875 30.209999999999997 85-89 25.424999999999997 20.01 24.45 30.115 90-94 24.455 24.815 25.215 25.515 95-99 19.405 25.39 26.919999999999998 28.285 100-104 21.34 24.81 19.82 34.03 105-109 23.95 24.435000000000002 18.315 33.300000000000004 110-114 22.45 25.1 22.195 30.255 115-119 20.73 21.584999999999997 28.799999999999997 28.884999999999998 120-124 22.78 28.21 24.875 24.135 125-129 25.169999999999998 21.105 21.725 32.0 130-134 27.22 17.88 22.845 32.055 135-139 27.400000000000002 18.345 24.03 30.225 140-144 27.05 20.47 18.43 34.050000000000004 145-149 30.320000000000004 23.369999999999997 21.14 25.169999999999998 150 24.5 27.35 20.75 27.400000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.5 28 0.5 29 1.5 30 3.5 31 4.5 32 5.5 33 6.0 34 7.5 35 11.5 36 27.0 37 40.5 38 38.0 39 39.5 40 34.5 41 39.5 42 53.0 43 44.0 44 60.0 45 148.5 46 209.0 47 201.5 48 178.0 49 150.0 50 155.0 51 323.5 52 326.5 53 182.0 54 156.5 55 125.5 56 114.5 57 92.5 58 89.5 59 236.5 60 241.5 61 81.5 62 79.5 63 93.0 64 102.0 65 91.5 66 43.0 67 16.5 68 28.0 69 35.0 70 19.5 71 12.0 72 12.5 73 20.5 74 13.5 75 2.0 76 2.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.75 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.275 #Duplication Level Percentage of deduplicated Percentage of total 1 67.69945778466305 21.85 2 13.632842757552286 8.799999999999999 3 5.189775367931835 5.025 4 2.5561580170410534 3.3000000000000003 5 1.549186676994578 2.5 6 1.7815646785437647 3.45 7 1.0844306738962044 2.45 8 1.0844306738962044 2.8000000000000003 9 0.23237800154918667 0.675 >10 4.570100697134005 30.75 >50 0.3872966692486445 8.075000000000001 >100 0.23237800154918667 10.325 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 173 4.324999999999999 No Hit CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA 129 3.225 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 111 2.775 No Hit CCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA 74 1.8499999999999999 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 73 1.825 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGA 68 1.7000000000000002 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 56 1.4000000000000001 No Hit CTCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTATAGGTCG 52 1.3 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 49 1.225 No Hit GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 47 1.175 No Hit CTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAAT 46 1.15 No Hit CCGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGA 42 1.05 No Hit CGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACT 41 1.0250000000000001 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 37 0.9249999999999999 No Hit CCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG 36 0.8999999999999999 No Hit ATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGG 34 0.8500000000000001 No Hit GCCGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTG 32 0.8 No Hit GGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGA 30 0.75 No Hit GCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCAG 30 0.75 No Hit GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCC 30 0.75 No Hit GTCGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGA 29 0.7250000000000001 No Hit CCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTA 29 0.7250000000000001 No Hit CTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCAC 28 0.7000000000000001 No Hit CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC 27 0.675 No Hit CCCTCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTATAGGT 26 0.65 No Hit GTCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGG 24 0.6 No Hit CCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGA 24 0.6 No Hit CCCGTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAA 24 0.6 No Hit CTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATC 23 0.575 No Hit CCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCT 23 0.575 No Hit GTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCC 22 0.5499999999999999 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 21 0.525 No Hit GTGGGAATTGTACTTCAAGGCGGCCCGCGCGGCTCTTTCACCGCGAGGGC 21 0.525 No Hit GTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGA 19 0.475 No Hit CTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGA 19 0.475 No Hit CCCCCGTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGG 18 0.44999999999999996 No Hit GGCCGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTT 18 0.44999999999999996 No Hit CCTCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTATAGGTC 17 0.42500000000000004 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGGCATCTCGGATG 17 0.42500000000000004 TruSeq Adapter, Index 18 (97% over 38bp) CCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAG 17 0.42500000000000004 No Hit GTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATT 17 0.42500000000000004 No Hit CCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTC 16 0.4 No Hit CCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAG 15 0.375 No Hit GTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTA 14 0.35000000000000003 No Hit CTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCA 14 0.35000000000000003 No Hit TGGGAATTGTACTTCAAGGCGGCCCGCGCGGCTCTTTCACCGCGAGGGCT 14 0.35000000000000003 No Hit GGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCA 14 0.35000000000000003 No Hit CCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCG 13 0.325 No Hit ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAG 13 0.325 No Hit CCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT 13 0.325 No Hit CCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTCCC 12 0.3 No Hit CTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCA 12 0.3 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGGCATCTCGTATG 12 0.3 TruSeq Adapter, Index 18 (97% over 38bp) CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCC 12 0.3 No Hit CCCGCCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTAT 12 0.3 No Hit CCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACC 12 0.3 No Hit GGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCC 12 0.3 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 11 0.27499999999999997 No Hit GGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTC 11 0.27499999999999997 No Hit CGGCCGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGT 11 0.27499999999999997 No Hit GCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGC 10 0.25 No Hit CCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCT 10 0.25 No Hit CCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACC 10 0.25 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 10 0.25 No Hit CTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATC 10 0.25 No Hit CTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTT 10 0.25 No Hit CGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT 10 0.25 No Hit CCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGCGAAC 9 0.22499999999999998 No Hit CCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAA 9 0.22499999999999998 No Hit GCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTT 9 0.22499999999999998 No Hit GGGAATTGTACTTCAAGGCGGCCCGCGCGGCTCTTTCACCGCGAGGGCTT 8 0.2 No Hit CTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC 8 0.2 No Hit CCCCACCTGACAATGTCTTCCGCCCGGATCGGCCGCCGAAGCGGCCTTGG 8 0.2 No Hit GTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAG 8 0.2 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 8 0.2 No Hit TTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCAC 8 0.2 No Hit CGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTC 8 0.2 No Hit CTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATC 8 0.2 No Hit GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT 8 0.2 No Hit CTCCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACC 8 0.2 No Hit CCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTAT 8 0.2 No Hit CCCCGTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGA 8 0.2 No Hit GGCAGAAATTTGAATGATGCGTCGCCAGCACGAAGGCCGTGCGATCCGTC 8 0.2 No Hit TCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAAC 8 0.2 No Hit GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATT 7 0.17500000000000002 No Hit CTCTGACTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACT 7 0.17500000000000002 No Hit GCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTG 7 0.17500000000000002 No Hit CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACT 7 0.17500000000000002 No Hit CCCCCCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCC 7 0.17500000000000002 No Hit GTCGACCTTTTATCTAATAAATGCGTCCCTTCCAGAAGTCGGGGTTTGTT 7 0.17500000000000002 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 7 0.17500000000000002 No Hit CCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGC 7 0.17500000000000002 No Hit CCGCCAATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCT 7 0.17500000000000002 No Hit TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCC 7 0.17500000000000002 No Hit GCCCTCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTATAGG 7 0.17500000000000002 No Hit GCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATC 7 0.17500000000000002 No Hit GCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGG 7 0.17500000000000002 No Hit CTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCGCCGAAGCGGCCTT 7 0.17500000000000002 No Hit CCCCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTC 6 0.15 No Hit TTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTT 6 0.15 No Hit CCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGG 6 0.15 No Hit GCCCCCGATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCACCGAG 6 0.15 No Hit AGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGC 6 0.15 No Hit CCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAA 6 0.15 No Hit GTCGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTG 6 0.15 No Hit CCCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGTCCTAA 6 0.15 No Hit CTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCC 6 0.15 No Hit CTCCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGC 6 0.15 No Hit CCCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTCC 6 0.15 No Hit CCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCA 6 0.15 No Hit CCGTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAAC 6 0.15 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCA 6 0.15 No Hit GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC 6 0.15 No Hit TCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGC 6 0.15 No Hit CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT 6 0.15 No Hit TGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAG 6 0.15 No Hit CTCCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACC 6 0.15 No Hit GCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCC 6 0.15 No Hit CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC 6 0.15 No Hit CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA 6 0.15 No Hit GCCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTC 6 0.15 No Hit GGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAAT 5 0.125 No Hit TCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGC 5 0.125 No Hit CTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTC 5 0.125 No Hit CCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCTCTATCCTACCA 5 0.125 No Hit CTCCGGTCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATC 5 0.125 No Hit CTCTCGATTCCGTGGCGCGGCTCAACGAAGCAGCCGCGCCGTCCTACCTA 5 0.125 No Hit CCCCGCCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTA 5 0.125 No Hit CCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTCCCG 5 0.125 No Hit GTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGCGA 5 0.125 No Hit CTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGAC 5 0.125 No Hit CTCCCCCCAGAACCCAAAAACTTTGATTTCTCATAAGGTGCTGGCGGAGT 5 0.125 No Hit GTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCAT 5 0.125 No Hit CGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCC 5 0.125 No Hit CTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTC 5 0.125 No Hit GGGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTC 5 0.125 No Hit CGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATG 5 0.125 No Hit CGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGAT 5 0.125 No Hit CTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGC 5 0.125 No Hit GACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGA 5 0.125 No Hit CTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.037500000000000006 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1625 0.0 0.0 0.0 0.0 64-65 0.2875 0.0 0.0 0.0 0.0 66-67 0.3 0.0 0.0 0.0 0.0 68-69 0.3 0.0 0.0 0.0 0.0 70-71 0.3375 0.0 0.0 0.0 0.0 72-73 0.3625 0.0 0.0 0.0 0.0 74-75 0.4375 0.0 0.0 0.0 0.0 76-77 0.5874999999999999 0.0 0.0 0.0 0.0 78-79 0.7 0.0 0.0 0.0 0.0 80-81 0.8375 0.0 0.0 0.0 0.0 82-83 1.0 0.0 0.0 0.0 0.0 84-85 1.125 0.0 0.0 0.0 0.0 86-87 1.3875 0.0 0.0 0.0 0.0 88-89 1.5499999999999998 0.0 0.0 0.0 0.0 90-91 1.6375000000000002 0.0 0.0 0.0 0.0 92-93 1.8125 0.0 0.0 0.0 0.0 94-95 2.2125 0.0 0.0 0.0 0.0 96-97 2.6875 0.0 0.0 0.0 0.0 98-99 3.0875 0.0 0.0 0.0 0.0 100-101 3.4749999999999996 0.0 0.0 0.0 0.0 102-103 4.2125 0.0 0.0 0.0 0.0 104-105 4.5625 0.0 0.0 0.0 0.0 106-107 5.137499999999999 0.0 0.0 0.0 0.0 108-109 5.7 0.0 0.0 0.0 0.0 110-111 6.375 0.0 0.0 0.0 0.0 112-113 6.975 0.0 0.0 0.0 0.0 114-115 7.7125 0.0 0.0 0.0 0.0 116-117 8.25 0.0 0.0 0.0 0.0 118-119 8.850000000000001 0.0 0.0 0.0 0.0 120-121 9.3875 0.0 0.0 0.0 0.0 122-123 9.9 0.0 0.0 0.0 0.0 124-125 10.5125 0.0 0.0 0.0 0.0 126-127 11.3875 0.0 0.0 0.0 0.0 128-129 12.35 0.0 0.0 0.0 0.0 130-131 13.1375 0.0 0.0 0.0 0.0 132-133 13.725 0.0 0.0 0.0 0.0 134-135 14.4875 0.0 0.0 0.0 0.0 136-137 15.2 0.0 0.0 0.0 0.0 138 15.925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGGTG 25 3.201967E-8 144.0 1 GCGGTGT 30 9.508585E-8 120.0 2 CGGTGTG 30 9.508585E-8 120.0 3 CCGGACT 20 3.687869E-4 108.0 3 CTCCCTA 20 3.687869E-4 108.0 8 GGACTCC 20 3.687869E-4 108.0 5 CGGACTC 20 3.687869E-4 108.0 4 ACTCCCT 20 3.687869E-4 108.0 7 TCCGGAC 20 3.687869E-4 108.0 2 GACTCCC 20 3.687869E-4 108.0 6 TCCCTAA 20 3.687869E-4 108.0 9 GGTGTGT 35 2.3845314E-7 102.85714 4 GTGTGTA 40 5.2840005E-7 90.0 5 TGTGTAC 45 1.0653384E-6 80.0 6 GTGTACA 50 1.9936197E-6 72.0 7 TGTACAA 50 1.9936197E-6 72.0 8 GTACAAA 55 3.5124358E-6 65.454544 9 CTCCGGA 40 0.005777437 54.0 1 TGACTCG 55 5.0997805E-6 20.945454 40-44 GTCAACG 55 5.0997805E-6 20.945454 25-29 >>END_MODULE SRR11845458 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR11845458_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.65425 37.0 37.0 37.0 37.0 37.0 2 35.4325 37.0 37.0 37.0 37.0 37.0 3 35.5795 37.0 37.0 37.0 37.0 37.0 4 35.456 37.0 37.0 37.0 37.0 37.0 5 35.726 37.0 37.0 37.0 37.0 37.0 6 35.6715 37.0 37.0 37.0 37.0 37.0 7 35.525 37.0 37.0 37.0 37.0 37.0 8 35.5705 37.0 37.0 37.0 37.0 37.0 9 35.6725 37.0 37.0 37.0 37.0 37.0 10-14 35.783500000000004 37.0 37.0 37.0 37.0 37.0 15-19 35.8032 37.0 37.0 37.0 37.0 37.0 20-24 35.7534 37.0 37.0 37.0 37.0 37.0 25-29 35.638299999999994 37.0 37.0 37.0 37.0 37.0 30-34 35.6152 37.0 37.0 37.0 37.0 37.0 35-39 35.6053 37.0 37.0 37.0 37.0 37.0 40-44 35.5522 37.0 37.0 37.0 37.0 37.0 45-49 35.6068 37.0 37.0 37.0 37.0 37.0 50-54 35.5077 37.0 37.0 37.0 37.0 37.0 55-59 35.4794 37.0 37.0 37.0 37.0 37.0 60-64 35.5515 37.0 37.0 37.0 37.0 37.0 65-69 35.4741 37.0 37.0 37.0 37.0 37.0 70-74 35.424 37.0 37.0 37.0 37.0 37.0 75-79 35.45700000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.4272 37.0 37.0 37.0 37.0 37.0 85-89 35.3837 37.0 37.0 37.0 37.0 37.0 90-94 35.5544 37.0 37.0 37.0 37.0 37.0 95-99 35.4577 37.0 37.0 37.0 37.0 37.0 100-104 35.332100000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.469100000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.3768 37.0 37.0 37.0 34.6 37.0 115-119 35.445800000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.3989 37.0 37.0 37.0 37.0 37.0 125-129 35.3318 37.0 37.0 37.0 34.6 37.0 130-134 35.0957 37.0 37.0 37.0 25.0 37.0 135-139 35.0373 37.0 37.0 37.0 25.0 37.0 140-144 34.698699999999995 37.0 37.0 37.0 25.0 37.0 145-149 34.6625 37.0 37.0 37.0 25.0 37.0 150 34.531 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 1.0 16 1.0 17 2.0 18 3.0 19 3.0 20 6.0 21 1.0 22 6.0 23 6.0 24 12.0 25 9.0 26 14.0 27 21.0 28 31.0 29 46.0 30 42.0 31 58.0 32 121.0 33 173.0 34 297.0 35 729.0 36 2247.0 37 169.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.730547910933204 16.23717788341256 8.581436077057793 34.450838128596445 2 30.175 23.05 30.8 15.975 3 24.0 25.424999999999997 27.750000000000004 22.825 4 28.325 29.225 20.424999999999997 22.025 5 31.7 28.249999999999996 20.549999999999997 19.5 6 23.799999999999997 34.775 24.099999999999998 17.325 7 25.1 21.6 33.1 20.200000000000003 8 27.474999999999998 19.400000000000002 27.775 25.35 9 27.875 21.95 26.85 23.325000000000003 10-14 29.335 27.12 22.035 21.51 15-19 28.88 25.759999999999998 23.935000000000002 21.425 20-24 29.599999999999998 24.985 23.25 22.165000000000003 25-29 29.630000000000003 25.490000000000002 22.905 21.975 30-34 30.869999999999997 25.785000000000004 21.154999999999998 22.189999999999998 35-39 27.500000000000004 26.419999999999998 23.465 22.615 40-44 28.634999999999998 25.495 23.35 22.52 45-49 27.810000000000002 26.724999999999998 23.064999999999998 22.400000000000002 50-54 28.64 27.735 21.12 22.505 55-59 30.06 24.985 23.03 21.925 60-64 30.659999999999997 25.205 22.255 21.88 65-69 28.68 24.85 23.235 23.235 70-74 29.7 25.869999999999997 22.33 22.1 75-79 29.37 23.885 23.565 23.18 80-84 29.525000000000002 24.805 22.21 23.46 85-89 27.82 24.43 22.96 24.79 90-94 30.165 24.555 22.53 22.75 95-99 29.005 25.564999999999998 21.785 23.645 100-104 30.595 26.615 20.82 21.97 105-109 31.28 24.055 22.375 22.29 110-114 31.455 26.33 20.385 21.83 115-119 30.635 25.264999999999997 21.955 22.145 120-124 31.724999999999998 24.455 20.585 23.235 125-129 32.565 23.915 20.685000000000002 22.835 130-134 33.035 25.665 19.355 21.945 135-139 33.86 23.11 19.43 23.599999999999998 140-144 33.69 24.15 20.085 22.075 145-149 33.739999999999995 22.425 20.745 23.09 150 33.324999999999996 24.8 21.0 20.875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 1.0 17 0.5 18 0.0 19 0.5 20 0.5 21 0.5 22 0.5 23 0.0 24 0.5 25 1.5 26 2.0 27 1.5 28 1.5 29 2.5 30 2.5 31 2.5 32 3.5 33 3.5 34 6.5 35 26.0 36 39.0 37 31.0 38 30.0 39 62.0 40 68.5 41 71.0 42 80.5 43 74.0 44 120.0 45 176.0 46 195.5 47 194.0 48 170.0 49 122.5 50 138.5 51 232.0 52 286.0 53 256.5 54 202.5 55 221.0 56 245.5 57 206.5 58 147.0 59 94.5 60 66.0 61 44.0 62 36.0 63 46.5 64 36.5 65 16.0 66 12.5 67 15.5 68 20.0 69 22.0 70 20.0 71 11.0 72 6.5 73 9.5 74 13.0 75 16.0 76 13.0 77 9.0 78 5.0 79 2.0 80 2.0 81 0.5 82 0.5 83 0.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 1.0 94 1.5 95 2.0 96 1.5 97 1.5 98 1.5 99 4.5 100 20.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.975 #Duplication Level Percentage of deduplicated Percentage of total 1 64.31350750416898 28.925 2 16.23123957754308 14.6 3 6.614785992217899 8.924999999999999 4 3.3907726514730405 6.1 5 2.2790439132851583 5.125 6 1.6675931072818233 4.5 7 1.1117287381878822 3.5000000000000004 8 0.9449694274596998 3.4000000000000004 9 0.44469149527515284 1.7999999999999998 >10 2.9460811561978875 21.65 >50 0.055586436909394105 1.4749999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCC 59 1.4749999999999999 No Hit GTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATAC 43 1.075 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 39 0.975 No Hit GCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATA 34 0.8500000000000001 No Hit CCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAA 34 0.8500000000000001 No Hit GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG 32 0.8 No Hit CTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCA 28 0.7000000000000001 No Hit CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT 23 0.575 No Hit CACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTC 23 0.575 No Hit GGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAG 23 0.575 No Hit CTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTT 23 0.575 No Hit GTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGT 22 0.5499999999999999 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 20 0.5 No Hit CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTC 19 0.475 No Hit CAACGAGTCTATAGCCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTC 18 0.44999999999999996 No Hit CGAGTCTATAGCCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTCATC 18 0.44999999999999996 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 17 0.42500000000000004 No Hit CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC 17 0.42500000000000004 No Hit GTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG 16 0.4 No Hit GTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG 16 0.4 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA 15 0.375 No Hit CTTTAAATAGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCACGGAA 15 0.375 No Hit GAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTA 15 0.375 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 14 0.35000000000000003 No Hit CCAGCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCA 14 0.35000000000000003 No Hit GGTCGTGCCTCCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCA 13 0.325 No Hit TTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTAC 13 0.325 No Hit GTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTACGA 13 0.325 No Hit CATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCCT 13 0.325 No Hit GGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCA 13 0.325 No Hit GACAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAAC 13 0.325 No Hit GTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTC 12 0.3 No Hit CTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATG 12 0.3 No Hit AACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTC 12 0.3 No Hit CATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAT 12 0.3 No Hit ACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACA 12 0.3 No Hit GGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTC 12 0.3 No Hit CCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGT 12 0.3 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTCTATAGCC 11 0.27499999999999997 No Hit ATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCC 11 0.27499999999999997 No Hit CAACTTTCGATGGTAGGATAGAGGCCTACCATGGTGGTGACGGGTGACGG 11 0.27499999999999997 No Hit CTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTA 11 0.27499999999999997 No Hit GTCGTGCCTCCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAA 11 0.27499999999999997 No Hit CTGATGTATTCAACGAGTCTATAGCCTTGGCCGACAGGCCCGGGTAATCT 11 0.27499999999999997 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 10 0.25 No Hit CAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACCCGGC 10 0.25 No Hit CGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACG 10 0.25 No Hit GCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTA 10 0.25 No Hit GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC 10 0.25 No Hit GGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATG 10 0.25 No Hit GAACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCA 10 0.25 No Hit AGCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAAT 10 0.25 No Hit GTGCCTCCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCC 10 0.25 No Hit GATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAA 10 0.25 No Hit GGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATC 9 0.22499999999999998 No Hit CTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCC 9 0.22499999999999998 No Hit GGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGT 9 0.22499999999999998 No Hit GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT 9 0.22499999999999998 No Hit TGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTCTATAGC 9 0.22499999999999998 No Hit GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA 9 0.22499999999999998 No Hit TTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAAC 9 0.22499999999999998 No Hit CAGCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAA 9 0.22499999999999998 No Hit ATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTA 8 0.2 No Hit CCTCAGCCTGCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTC 8 0.2 No Hit TTTGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCA 8 0.2 No Hit GGATGGCGCTGAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGCGCCA 8 0.2 No Hit AGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTA 8 0.2 No Hit TGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA 8 0.2 No Hit GACCTCAGCCTGCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCT 8 0.2 No Hit CAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGG 8 0.2 No Hit GACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAA 8 0.2 No Hit CATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTTAGGACTCCGC 8 0.2 No Hit CGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAG 8 0.2 No Hit GGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTGCAA 8 0.2 No Hit CGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCT 8 0.2 No Hit CTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACCCGGCCGT 8 0.2 No Hit CGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAA 8 0.2 No Hit GTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGG 8 0.2 No Hit CGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 8 0.2 No Hit ATTCAACGAGTCTATAGCCTTGGCCGACAGGCCCGGGTAATCTTTGAAAT 7 0.17500000000000002 No Hit TGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAA 7 0.17500000000000002 No Hit CCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTTCGTTGAGC 7 0.17500000000000002 No Hit GGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAA 7 0.17500000000000002 No Hit CCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGT 7 0.17500000000000002 No Hit TGACCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAG 7 0.17500000000000002 No Hit GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA 7 0.17500000000000002 No Hit CTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCA 7 0.17500000000000002 No Hit GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA 7 0.17500000000000002 No Hit TAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCA 7 0.17500000000000002 No Hit CGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTGACCC 7 0.17500000000000002 No Hit GAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTGACCCTCCGCGG 7 0.17500000000000002 No Hit AGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCA 7 0.17500000000000002 No Hit CGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTT 7 0.17500000000000002 No Hit GTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGC 7 0.17500000000000002 No Hit CTCGTCCCTTCTACCGGCGATGCGCTCCTGGCCTTAACTGGCCGGGTCGT 7 0.17500000000000002 No Hit CGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATT 7 0.17500000000000002 No Hit AATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAG 7 0.17500000000000002 No Hit CAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAG 7 0.17500000000000002 No Hit CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG 7 0.17500000000000002 No Hit CTGCAAAACCCGGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATC 6 0.15 No Hit GCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCT 6 0.15 No Hit GATGTATTCAACGAGTCTATAGCCTTGGCCGACAGGCCCGGGTAATCTTT 6 0.15 No Hit GACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAA 6 0.15 No Hit GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC 6 0.15 No Hit GTTCGAGTGAGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC 6 0.15 No Hit CGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGC 6 0.15 No Hit GTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGA 6 0.15 No Hit ATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGT 6 0.15 No Hit GTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCAC 6 0.15 No Hit CAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA 6 0.15 No Hit CGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCA 6 0.15 No Hit GAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGG 6 0.15 No Hit GAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTG 6 0.15 No Hit GTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAAC 6 0.15 No Hit AGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCTTCCAGGCC 6 0.15 No Hit ATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTA 6 0.15 No Hit GGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAA 6 0.15 No Hit CTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGG 6 0.15 No Hit GGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCG 6 0.15 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAA 6 0.15 No Hit CGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTG 6 0.15 No Hit AGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGA 6 0.15 No Hit CGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAA 6 0.15 No Hit ATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG 6 0.15 No Hit ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC 6 0.15 No Hit GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG 6 0.15 No Hit CTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAACAG 6 0.15 No Hit CAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGC 6 0.15 No Hit AGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGA 6 0.15 No Hit CGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGAT 5 0.125 No Hit CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCCTACCATGG 5 0.125 No Hit GGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAG 5 0.125 No Hit CTTTCGATGGTAGGATAGAGGCCTACCATGGTGGTGACGGGTGACGGAGA 5 0.125 No Hit CGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCCGGGGCGCGAGCCC 5 0.125 No Hit AGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGG 5 0.125 No Hit GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTA 5 0.125 No Hit GGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAG 5 0.125 No Hit CAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCT 5 0.125 No Hit CCTCGTCCCTTCTACCGGCGATGCGCTCCTGGCCTTAACTGGCCGGGTCG 5 0.125 No Hit CACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTG 5 0.125 No Hit CCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACCCGGCCGTCGGGGC 5 0.125 No Hit CAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCT 5 0.125 No Hit CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG 5 0.125 No Hit GGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCT 5 0.125 No Hit AAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGT 5 0.125 No Hit CAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA 5 0.125 No Hit TGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCAT 5 0.125 No Hit CAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACT 5 0.125 No Hit GGTGACCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCTTCCAGGCCA 5 0.125 No Hit TGACAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATT 5 0.125 No Hit CCCTCCGCGGCCAGCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAA 5 0.125 No Hit GAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGC 5 0.125 No Hit ACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGC 5 0.125 No Hit TGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCC 5 0.125 No Hit CTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTATTTGCTA 5 0.125 No Hit GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT 5 0.125 No Hit TGCCTCCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCT 5 0.125 No Hit GTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGA 5 0.125 No Hit CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT 5 0.125 No Hit CCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAG 5 0.125 No Hit AGACAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAA 5 0.125 No Hit GTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGA 5 0.125 No Hit CAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCG 5 0.125 No Hit CACTGATGTATTCAACGAGTCTATAGCCTTGGCCGACAGGCCCGGGTAAT 5 0.125 No Hit GGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTG 5 0.125 No Hit ATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATC 5 0.125 No Hit GCCAGCTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGC 5 0.125 No Hit CGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCA 5 0.125 No Hit CGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGGTGACCCTCCGCG 5 0.125 No Hit CTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.037500000000000006 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1625 0.0 0.0 0.0 0.0 64-65 0.2875 0.0 0.0 0.0 0.0 66-67 0.3 0.0 0.0 0.0 0.0 68-69 0.3 0.0 0.0 0.0 0.0 70-71 0.3375 0.0 0.0 0.0 0.0 72-73 0.3625 0.0 0.0 0.0 0.0 74-75 0.4375 0.0 0.0 0.0 0.0 76-77 0.6125 0.0 0.0 0.0 0.0 78-79 0.725 0.0 0.0 0.0 0.0 80-81 0.8625 0.0 0.0 0.0 0.0 82-83 1.025 0.0 0.0 0.0 0.0 84-85 1.15 0.0 0.0 0.0 0.0 86-87 1.425 0.0 0.0 0.0 0.0 88-89 1.6 0.0 0.0 0.0 0.0 90-91 1.7 0.0 0.0 0.0 0.0 92-93 1.8875 0.0 0.0 0.0 0.0 94-95 2.3 0.0 0.0 0.0 0.0 96-97 2.8 0.0 0.0 0.0 0.0 98-99 3.2125 0.0 0.0 0.0 0.0 100-101 3.5999999999999996 0.0 0.0 0.0 0.0 102-103 4.324999999999999 0.0 0.0 0.0 0.0 104-105 4.6875 0.0 0.0 0.0 0.0 106-107 5.25 0.0 0.0 0.0 0.0 108-109 5.7875 0.0 0.0 0.0 0.0 110-111 6.5 0.0 0.0 0.0 0.0 112-113 7.125 0.0 0.0 0.0 0.0 114-115 7.9 0.0 0.0 0.0 0.0 116-117 8.4375 0.0 0.0 0.0 0.0 118-119 9.024999999999999 0.0 0.0 0.0 0.0 120-121 9.5625 0.0 0.0 0.0 0.0 122-123 10.075 0.0 0.0 0.0 0.0 124-125 10.6875 0.0 0.0 0.0 0.0 126-127 11.6125 0.0 0.0 0.0 0.0 128-129 12.5625 0.0 0.0 0.0 0.0 130-131 13.337499999999999 0.0 0.0 0.0 0.0 132-133 13.962499999999999 0.0 0.0 0.0 0.0 134-135 14.675 0.0 0.0 0.0 0.0 136-137 15.4375 0.0 0.0 0.0 0.0 138 16.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACCAG 10 0.006973645 144.0 2 GGTCCAG 10 0.006973645 144.0 8 CTTACCA 10 0.006973645 144.0 1 GTCCAGA 10 0.006973645 144.0 9 AGGTCCA 10 0.006973645 144.0 7 CAGGTCC 10 0.006973645 144.0 6 TACCAGG 10 0.006973645 144.0 3 CCAGGTC 10 0.006973645 144.0 5 ACCAGGT 10 0.006973645 144.0 4 GGAAGTT 40 6.0911432E-5 72.0 1 TTTGAGG 45 1.0917089E-4 64.0 6 GAGGCAA 45 1.0917089E-4 64.0 9 GTTTGAG 45 1.0917089E-4 64.0 5 TTGAGGC 45 1.0917089E-4 64.0 7 TGAGGCA 45 1.0917089E-4 64.0 8 AGTTTGA 45 1.0917089E-4 64.0 4 AAGTTTG 45 1.0917089E-4 64.0 3 GAAGTTT 50 1.838823E-4 57.6 2 ATCGGAA 60 0.0047032754 14.4 140-144 >>END_MODULE Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105580 spots for SRR11845458.sra Written 1105580 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra Read 1105572 spots for SRR11845458.sra Written 1105572 spots for SRR11845458.sra SRR ids: ['SRR11845458.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ww4xnevp SRR11845458.sra spots: 22111448 blocks: [[1, 1105572], [1105573, 2211144], [2211145, 3316716], [3316717, 4422288], [4422289, 5527860], [5527861, 6633432], [6633433, 7739004], [7739005, 8844576], [8844577, 9950148], [9950149, 11055720], [11055721, 12161292], [12161293, 13266864], [13266865, 14372436], [14372437, 15478008], [15478009, 16583580], [16583581, 17689152], [17689153, 18794724], [18794725, 19900296], [19900297, 21005868], [21005869, 22111448]] SRR11845458 file size 7449550 SRR11845458 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11845458 SRR11845458_1.fastq SRR11845458_2.fastq Input file: SRR11845458_1.fastq Paired file: SRR11845458_2.fastq trimmed: SRR11845458-trimmed-pair1.fastq, SRR11845458-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Feb 13 16:07:47 2025 >> started Thu Feb 13 16:08:10 2025 >> done (23.198s) 22111448 read pairs processed; of these: 12 ( 0.00%) short read pairs filtered out after trimming by size control 225073 ( 1.02%) empty read pairs filtered out after trimming by size control 21886363 (98.98%) read pairs available; of these: 4454606 (20.35%) trimmed read pairs available after processing 17431757 (79.65%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 0 0.00% 21 4 0.00% 22 8 0.00% 23 9 0.00% 24 7 0.00% 25 9 0.00% 26 20 0.00% 27 41 0.00% 28 61 0.00% 29 86 0.00% 30 86 0.00% 31 187 0.00% 32 209 0.00% 33 332 0.00% 34 339 0.00% 35 475 0.00% 36 443 0.00% 37 482 0.00% 38 567 0.00% 39 892 0.00% 40 1112 0.01% 41 1123 0.01% 42 1361 0.01% 43 1097 0.01% 44 1405 0.01% 45 1527 0.01% 46 1532 0.01% 47 1909 0.01% 48 2008 0.01% 49 2294 0.01% 50 2124 0.01% 51 2435 0.01% 52 2936 0.01% 53 2852 0.01% 54 2983 0.01% 55 3286 0.02% 56 3596 0.02% 57 3749 0.02% 58 4655 0.02% 59 5490 0.03% 60 6515 0.03% 61 6144 0.03% 62 6352 0.03% 63 5953 0.03% 64 6497 0.03% 65 5876 0.03% 66 7049 0.03% 67 6757 0.03% 68 6817 0.03% 69 7387 0.03% 70 7621 0.03% 71 8485 0.04% 72 8829 0.04% 73 9034 0.04% 74 9918 0.05% 75 10443 0.05% 76 15979 0.07% 77 17172 0.08% 78 14193 0.06% 79 15187 0.07% 80 14795 0.07% 81 17409 0.08% 82 17653 0.08% 83 17474 0.08% 84 20720 0.09% 85 23313 0.11% 86 18376 0.08% 87 20772 0.09% 88 21194 0.10% 89 20954 0.10% 90 22605 0.10% 91 25555 0.12% 92 30984 0.14% 93 33231 0.15% 94 34422 0.16% 95 39915 0.18% 96 40222 0.18% 97 38252 0.17% 98 45111 0.21% 99 49413 0.23% 100 48293 0.22% 101 47510 0.22% 102 54080 0.25% 103 53695 0.25% 104 54906 0.25% 105 55693 0.25% 106 52300 0.24% 107 62880 0.29% 108 58300 0.27% 109 60168 0.27% 110 62075 0.28% 111 70906 0.32% 112 58262 0.27% 113 61407 0.28% 114 66199 0.30% 115 77421 0.35% 116 63855 0.29% 117 67417 0.31% 118 64276 0.29% 119 61507 0.28% 120 62694 0.29% 121 67498 0.31% 122 77621 0.35% 123 82961 0.38% 124 72571 0.33% 125 80764 0.37% 126 104767 0.48% 127 96512 0.44% 128 105313 0.48% 129 82933 0.38% 130 67464 0.31% 131 71523 0.33% 132 80030 0.37% 133 96213 0.44% 134 93741 0.43% 135 88194 0.40% 136 94900 0.43% 137 81236 0.37% 138 91140 0.42% 139 89686 0.41% 140 93689 0.43% 141 86187 0.39% 142 73141 0.33% 143 72826 0.33% 144 68708 0.31% 145 67186 0.31% 146 74375 0.34% 147 83161 0.38% 148 83590 0.38% 149 74521 0.34% 150 17431757 79.65% 21886363 reads passed initial QC criterion=sequence-density sequence-density=0.73 sequence-density-rank=1 fanout-score=26.40 fanout-score-rank=18 prefix-density=19.12 prefix-fanout=1.0 sequence=AAGATTACCCGAGCCTGTCGGCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=617.14 fanout-score-rank=1 prefix-density=4.60 prefix-fanout=1.0 sequence=ACATGGAACCTGTCCCCTCTTCG criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=27.17 fanout-score-rank=31 prefix-density=11.84 prefix-fanout=1.0 sequence=GGCCGACAGGCTCGGGTAATCTT criterion=fanout-score sequence-density=0.11 sequence-density-rank=16 fanout-score=271.85 fanout-score-rank=1 prefix-density=16.90 prefix-fanout=1.8 sequence=TTCAACGAGGACTTCCTAGTAAG SRR11845458 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 13 16:08:51 Started mapping on | Feb 13 16:08:51 Finished on | Feb 13 16:16:08 Mapping speed, Million of reads per hour | 180.30 Number of input reads | 21886363 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 1923375 Uniquely mapped reads % | 8.79% Average mapped length | 277.82 Number of splices: Total | 704290 Number of splices: Annotated (sjdb) | 681526 Number of splices: GT/AG | 690548 Number of splices: GC/AG | 9402 Number of splices: AT/AC | 832 Number of splices: Non-canonical | 3508 Mismatch rate per base, % | 0.32% Deletion rate per base | 0.02% Deletion average length | 2.61 Insertion rate per base | 0.02% Insertion average length | 2.96 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 343318 % of reads mapped to multiple loci | 1.57% Number of reads mapped to too many loci | 16478093 % of reads mapped to too many loci | 75.29% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.13% % of reads unmapped: other | 13.23% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 19619670 19619670 19619670 N_multimapping 343318 343318 343318 N_noFeature 549109 1758542 685433 N_ambiguous 36155 423 7264 UnstrandedReadsAssigned:1338111 PositiveStrandReadsAssigned:164410 NegativeStrandReadsAssigned:1230678 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=149 echo kmer=145 SRR11845458 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR11845458-trimmed-pair1.fastq SRR11845458-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,886,363 reads, 10,651,057 reads pseudoaligned [quant] estimated average fragment length: 198.416 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,054 rounds 52401 SRR11845458.ke.tsv 34699 SRR11845458.se.tsv 87100 total ==> SRR11845458.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1820.58 55 1.94469 Potri.005G024800.1.v4.1 1035 837.584 2 0.153709 Potri.004G059700.1.v4.1 961 763.584 3 0.252908 Potri.007G009000.2.v4.1 1416 1218.58 0 0 Potri.003G141000.2.v4.1 2943 2745.58 12 0.281349 Potri.016G087400.1.v4.1 270 98.1047 11 7.21774 Potri.015G069301.1.v4.1 564 366.584 0 0 Potri.010G195200.1.v4.1 1773 1575.58 16 0.653697 Potri.012G127500.1.v4.1 977 779.584 198 16.3493 ==> SRR11845458.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 214 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 13 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 4 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR11845458 completed mapping pipeline successfully