Starting /dee2/code/volunteer_pipeline.sh SRR12192578
    current disk space = 3085094895616
    free memory = 1580593784 
SRR12192578 SRAfilesize
4698c96caab50be7cc709bd5f895e195  SRR12192578.sra
SRR12192578.sra file validated
SRR12192578 is single end
SRR12192578 is conventional basespace
SRR12192578 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR12192578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.20375	32.0	2.0	32.0	2.0	32.0
2	31.65875	32.0	32.0	32.0	32.0	32.0
3	34.17875	32.0	32.0	37.0	32.0	37.0
4	36.03875	37.0	37.0	37.0	32.0	37.0
5	36.56625	37.0	37.0	37.0	37.0	37.0
6	40.1605	41.0	41.0	41.0	37.0	41.0
7	40.2545	41.0	41.0	41.0	37.0	41.0
8	40.42275	41.0	41.0	41.0	41.0	41.0
9	40.31725	41.0	41.0	41.0	41.0	41.0
10-14	40.376	41.0	41.0	41.0	41.0	41.0
15-19	40.37474999999999	41.0	41.0	41.0	41.0	41.0
20-24	40.3976	41.0	41.0	41.0	41.0	41.0
25-29	40.368300000000005	41.0	41.0	41.0	41.0	41.0
30-34	40.245250000000006	41.0	41.0	41.0	38.6	41.0
35-39	40.3588	41.0	41.0	41.0	41.0	41.0
40-44	40.21295	41.0	41.0	41.0	40.2	41.0
45-49	40.291	41.0	41.0	41.0	41.0	41.0
50-54	40.24615	41.0	41.0	41.0	39.4	41.0
55-59	40.19755	41.0	41.0	41.0	40.2	41.0
60-64	40.085449999999994	41.0	41.0	41.0	39.4	41.0
65-69	40.08475	41.0	41.0	41.0	37.8	41.0
70-74	40.035650000000004	41.0	41.0	41.0	37.0	41.0
75-79	39.807599999999994	41.0	41.0	41.0	37.0	41.0
80-84	40.0655	41.0	41.0	41.0	37.0	41.0
85-89	40.11965	41.0	41.0	41.0	37.8	41.0
90-94	40.069100000000006	41.0	41.0	41.0	37.0	41.0
95-99	39.9506	41.0	41.0	41.0	37.0	41.0
100-104	39.9307	41.0	41.0	41.0	37.0	41.0
105-109	39.882400000000004	41.0	41.0	41.0	37.0	41.0
110-114	39.8235	41.0	41.0	41.0	37.0	41.0
115-119	39.7165	41.0	41.0	41.0	37.0	41.0
120-124	39.4209	41.0	41.0	41.0	37.0	41.0
125-129	39.27225	41.0	41.0	41.0	37.0	41.0
130-134	39.1678	41.0	41.0	41.0	37.0	41.0
135-139	39.1441	41.0	41.0	41.0	37.0	41.0
140-144	38.97055	41.0	41.0	41.0	37.0	41.0
145-149	38.707	41.0	40.2	41.0	33.0	41.0
150	38.67025	41.0	41.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	3.0
25	2.0
26	3.0
27	7.0
28	9.0
29	11.0
30	20.0
31	20.0
32	19.0
33	39.0
34	45.0
35	56.0
36	79.0
37	150.0
38	213.0
39	546.0
40	2778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.730703259005146	15.09433962264151	15.480274442538594	40.694682675814754
2	21.525	21.224999999999998	34.725	22.525000000000002
3	23.7	23.75	26.525	26.025
4	25.494120590442833	28.821616212159118	22.34175631723793	23.34250688016012
5	26.224999999999998	32.975	21.825	18.975
6	19.375	37.375	23.549999999999997	19.7
7	18.7	18.775	41.025	21.5
8	20.525	23.150000000000002	28.375	27.950000000000003
9	21.05	21.95	31.574999999999996	25.424999999999997
10-14	22.37	28.310000000000002	25.814999999999998	23.505000000000003
15-19	22.975	27.305	26.619999999999997	23.1
20-24	22.45	27.61	26.784999999999997	23.155
25-29	23.075000000000003	27.005000000000003	26.345000000000002	23.575
30-34	22.93	26.61	26.93	23.53
35-39	23.735	26.855	26.340000000000003	23.07
40-44	23.189999999999998	26.795	26.590000000000003	23.425
45-49	23.145	26.224999999999998	27.134999999999998	23.494999999999997
50-54	23.455000000000002	26.895000000000003	27.24	22.41
55-59	23.255	26.66	26.625	23.46
60-64	23.115	26.650000000000002	27.084999999999997	23.150000000000002
65-69	22.495	27.35	26.5	23.655
70-74	22.814999999999998	27.245	26.369999999999997	23.57
75-79	23.77	26.650000000000002	25.965	23.615
80-84	23.425	26.895000000000003	26.565	23.115
85-89	23.54	27.04	26.69	22.73
90-94	23.265	26.665	26.36	23.71
95-99	23.315	26.740000000000002	27.245	22.7
100-104	23.297473104828622	26.8951713785339	26.584938704028023	23.222416812609456
105-109	22.830000000000002	26.645000000000003	27.235	23.29
110-114	22.965669102191974	26.508857972174958	27.08938044239816	23.436092483234912
115-119	22.984193677470987	26.800720288115247	26.65566226490596	23.559423769507802
120-124	23.49554420746971	26.22909782717533	27.075197757084208	23.200160208270752
125-129	23.082699239086903	26.39167000400481	27.588105726872246	22.937525030036042
130-134	23.02032235459005	26.80949043948343	27.08479327259986	23.08539393332666
135-139	23.029969480162105	27.36778906289088	26.10696953019463	23.49527192675239
140-144	23.905	27.51	26.1	22.485
145-149	24.107410741074105	27.63776377637764	25.6025602560256	22.65226522652265
150	23.05	27.025	26.450000000000003	23.474999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.5
24	2.0
25	1.0
26	2.5
27	4.5
28	5.5
29	10.5
30	17.0
31	21.5
32	23.5
33	25.0
34	28.0
35	41.0
36	57.5
37	77.0
38	114.5
39	154.0
40	185.0
41	208.5
42	226.5
43	260.0
44	271.0
45	254.0
46	254.0
47	257.0
48	214.5
49	165.0
50	152.0
51	124.0
52	91.5
53	79.0
54	72.0
55	53.0
56	41.0
57	42.0
58	39.0
59	43.5
60	45.0
61	38.5
62	34.0
63	32.0
64	45.0
65	51.0
66	38.0
67	36.5
68	32.0
69	17.0
70	6.5
71	0.5
72	0.5
73	0.5
74	1.5
75	2.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	41.699999999999996
2	0.0
3	0.0
4	0.075
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.075
105-109	0.0
110-114	0.09
115-119	0.04
120-124	0.13
125-129	0.12
130-134	0.11
135-139	0.065
140-144	0.0
145-149	0.01
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.72500000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.21311047270828	92.10000000000001
2	3.3951423348132668	6.5
3	0.2872812744841995	0.8250000000000001
4	0.05223295899712719	0.2
5	0.026116479498563595	0.125
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.026116479498563595	0.25
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAGAGGATCCCTCGAGGTCGACATGGTGAGCAAGGGCGAGGAGCTGTTC	10	0.25	No Hit
NTAGAGGATCCCTCGAGGTCGACATGGTGAGCAAGGGCGAGGAGCTGTTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.2875	0.0	0.0	0.0	0.0
136-137	1.375	0.0	0.0	0.0	0.0
138	2.175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	20	0.0063356147	28.615	140-144
>>END_MODULE
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104641 READS because READLEN < 1
Read 1104641 spots for SRR12192578.sra
Written 1104641 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
Rejected 1104627 READS because READLEN < 1
Read 1104627 spots for SRR12192578.sra
Written 1104627 spots for SRR12192578.sra
SRR ids: ['SRR12192578.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_u9isausd
SRR12192578.sra spots: 22092554
blocks: [[1, 1104627], [1104628, 2209254], [2209255, 3313881], [3313882, 4418508], [4418509, 5523135], [5523136, 6627762], [6627763, 7732389], [7732390, 8837016], [8837017, 9941643], [9941644, 11046270], [11046271, 12150897], [12150898, 13255524], [13255525, 14360151], [14360152, 15464778], [15464779, 16569405], [16569406, 17674032], [17674033, 18778659], [18778660, 19883286], [19883287, 20987913], [20987914, 22092554]]
SRR12192578 file size 7443166
SRR12192578 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR12192578 SRR12192578_1.fastq
Input file:	SRR12192578_1.fastq
trimmed:	SRR12192578-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Fri Feb 14 06:48:34 2025 >> started

Fri Feb 14 06:48:47 2025 >> done (13.010s)
22092554 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
22092554 (100.00%) reads available; of these:
  138509 ( 0.63%) trimmed reads available after processing
21954045 (99.37%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 30	       1	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       1	  0.00%
 37	       0	  0.00%
 38	       1	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       1	  0.00%
 45	       1	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       1	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       1	  0.00%
 53	       1	  0.00%
 54	       0	  0.00%
 55	       2	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       1	  0.00%
 59	       0	  0.00%
 60	       2	  0.00%
 61	       0	  0.00%
 62	       1	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       1	  0.00%
 66	       4	  0.00%
 67	       3	  0.00%
 68	       3	  0.00%
 69	       0	  0.00%
 70	       0	  0.00%
 71	       0	  0.00%
 72	       0	  0.00%
 73	       2	  0.00%
 74	       0	  0.00%
 75	       1	  0.00%
 76	       1	  0.00%
 77	       2	  0.00%
 78	       0	  0.00%
 79	       1	  0.00%
 80	       0	  0.00%
 81	       1	  0.00%
 82	       1	  0.00%
 83	       1	  0.00%
 84	       1	  0.00%
 85	       0	  0.00%
 86	       1	  0.00%
 87	       1	  0.00%
 88	       1	  0.00%
 89	       1	  0.00%
 90	       1	  0.00%
 91	       2	  0.00%
 92	       5	  0.00%
 93	       1	  0.00%
 94	       2	  0.00%
 95	       3	  0.00%
 96	       4	  0.00%
 97	       2	  0.00%
 98	       3	  0.00%
 99	       2	  0.00%
100	       4	  0.00%
101	       2	  0.00%
102	       5	  0.00%
103	       3	  0.00%
104	       3	  0.00%
105	       5	  0.00%
106	       8	  0.00%
107	       6	  0.00%
108	       3	  0.00%
109	      17	  0.00%
110	       7	  0.00%
111	      12	  0.00%
112	      12	  0.00%
113	       7	  0.00%
114	      11	  0.00%
115	      18	  0.00%
116	      18	  0.00%
117	      22	  0.00%
118	      10	  0.00%
119	       0	  0.00%
120	       0	  0.00%
121	       0	  0.00%
122	       0	  0.00%
123	       0	  0.00%
124	       0	  0.00%
125	       0	  0.00%
126	       0	  0.00%
127	       0	  0.00%
128	       0	  0.00%
129	       0	  0.00%
130	       0	  0.00%
131	       0	  0.00%
132	       0	  0.00%
133	       0	  0.00%
134	       0	  0.00%
135	       0	  0.00%
136	       0	  0.00%
137	       0	  0.00%
138	       0	  0.00%
139	       1	  0.00%
140	       7	  0.00%
141	      13	  0.00%
142	      32	  0.00%
143	      95	  0.00%
144	     209	  0.00%
145	     481	  0.00%
146	    1220	  0.01%
147	    3877	  0.02%
148	   17153	  0.08%
149	  115183	  0.52%
150	21954045	 99.37%
22092554 reads passed initial QC


criterion=sequence-density
sequence-density=1.56
sequence-density-rank=1
fanout-score=80.36
fanout-score-rank=1
prefix-density=2.81
prefix-fanout=44.5
sequence=AGATCGGAAGAGCACA


criterion=fanout-score
sequence-density=1.56
sequence-density-rank=1
fanout-score=80.36
fanout-score-rank=1
prefix-density=2.81
prefix-fanout=44.5
sequence=AGATCGGAAGAGCACA
                                 Started job on |	Feb 14 06:49:21
                             Started mapping on |	Feb 14 06:49:21
                                    Finished on |	Feb 14 06:51:04
       Mapping speed, Million of reads per hour |	772.17

                          Number of input reads |	22092554
                      Average input read length |	149
                                    UNIQUE READS:
                   Uniquely mapped reads number |	18312104
                        Uniquely mapped reads % |	82.89%
                          Average mapped length |	149.02
                       Number of splices: Total |	9369110
            Number of splices: Annotated (sjdb) |	9152053
                       Number of splices: GT/AG |	9222906
                       Number of splices: GC/AG |	120395
                       Number of splices: AT/AC |	7046
               Number of splices: Non-canonical |	18763
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.90
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.70
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	986259
             % of reads mapped to multiple loci |	4.46%
        Number of reads mapped to too many loci |	88717
             % of reads mapped to too many loci |	0.40%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.23%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2794191	2794191	2794191
N_multimapping	986259	986259	986259
N_noFeature	599888	9547604	9302404
N_ambiguous	118244	28633	28040
UnstrandedReadsAssigned:17593972 PositiveStrandReadsAssigned:8735867 NegativeStrandReadsAssigned:8981660
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR12192578 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in single-end mode
[quant] will process file 1: SRR12192578-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,092,554 reads, 18,522,249 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,106 rounds

  52401 SRR12192578.ke.tsv
  34699 SRR12192578.se.tsv
  87100 total
==> SRR12192578.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1919	4444.48	156.866
Potri.005G024800.1.v4.1	1035	936	576	41.6801
Potri.004G059700.1.v4.1	961	862	3	0.23572
Potri.007G009000.2.v4.1	1416	1317	0	0
Potri.003G141000.2.v4.1	2943	2844	415.351	9.89162
Potri.016G087400.1.v4.1	270	171	946	374.694
Potri.015G069301.1.v4.1	564	465	0	0
Potri.010G195200.1.v4.1	1773	1674	451.944	18.2857
Potri.012G127500.1.v4.1	977	878	2671	206.045

==> SRR12192578.se.tsv <==
Potri.001G166300.v4.1	0
Potri.001G448400.v4.1	1
Potri.001G233950.v4.1	0
Potri.001G122700.v4.1	173
Potri.001G212900.v4.1	0
Potri.001G182400.v4.1	0
Potri.001G256600.v4.1	0
Potri.001G040500.v4.1	10
Potri.001G416900.v4.1	6
Potri.001G452600.v4.1	0
SRR12192578 completed mapping pipeline successfully
