Starting /dee2/code/volunteer_pipeline.sh SRR12560176
    current disk space = 3056358842368
    free memory = 1464182828 
SRR12560176 SRAfilesize
119e825d4f743ff6cd1748f9fc458def  SRR12560176.sra
SRR12560176.sra file validated
SRR12560176 is single end
SRR12560176 is conventional basespace
SRR12560176 read1 length is 114-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR12560176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	114-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89125	32.0	32.0	32.0	32.0	32.0
2	31.22625	32.0	32.0	32.0	32.0	32.0
3	34.58875	37.0	32.0	37.0	32.0	37.0
4	36.09325	37.0	37.0	37.0	32.0	37.0
5	35.90825	37.0	37.0	37.0	32.0	37.0
6	39.19275	41.0	41.0	41.0	37.0	41.0
7	39.53825	41.0	41.0	41.0	37.0	41.0
8	39.66825	41.0	41.0	41.0	37.0	41.0
9	39.2845	41.0	41.0	41.0	37.0	41.0
10-14	39.50865	41.0	41.0	41.0	37.0	41.0
15-19	39.592150000000004	41.0	41.0	41.0	37.0	41.0
20-24	39.46175	41.0	41.0	41.0	37.0	41.0
25-29	39.07755	41.0	41.0	41.0	37.0	41.0
30-34	38.9744	41.0	41.0	41.0	35.0	41.0
35-39	39.2769	41.0	41.0	41.0	37.0	41.0
40-44	39.28365000000001	41.0	41.0	41.0	36.0	41.0
45-49	39.2119	41.0	41.0	41.0	37.0	41.0
50-54	38.9843	41.0	41.0	41.0	36.0	41.0
55-59	38.9267	41.0	41.0	41.0	34.0	41.0
60-64	38.72265	41.0	40.2	41.0	34.0	41.0
65-69	38.662400000000005	41.0	39.4	41.0	33.0	41.0
70-74	38.63645	41.0	40.2	41.0	32.0	41.0
75-79	38.70755	41.0	39.4	41.0	34.0	41.0
80-84	38.915350000000004	41.0	41.0	41.0	33.0	41.0
85-89	38.9028	41.0	41.0	41.0	34.0	41.0
90-94	39.0676	41.0	41.0	41.0	35.0	41.0
95-99	38.8517	41.0	41.0	41.0	33.0	41.0
100-104	38.81955	41.0	41.0	41.0	35.0	41.0
105-109	38.65155	41.0	41.0	41.0	32.0	41.0
110-114	38.538	41.0	39.4	41.0	32.0	41.0
115-119	38.16959239809953	41.0	37.0	41.0	32.0	41.0
120-124	38.0944230805075	41.0	37.0	41.0	32.0	41.0
125-129	37.69583222201436	41.0	37.0	41.0	30.0	41.0
130-134	37.158866694919816	41.0	37.0	41.0	27.0	41.0
135-139	36.63806985686837	41.0	37.0	41.0	25.0	41.0
140-144	36.4225082964129	41.0	37.0	41.0	24.0	41.0
145-149	36.446608884583426	41.0	37.0	41.0	25.0	41.0
150-151	36.97519416566414	39.0	34.5	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	6.0
23	5.0
24	8.0
25	9.0
26	12.0
27	9.0
28	24.0
29	30.0
30	39.0
31	54.0
32	68.0
33	120.0
34	118.0
35	152.0
36	208.0
37	305.0
38	439.0
39	701.0
40	1693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.700000000000003	11.975	15.65	44.675
2	24.325	15.875	35.925000000000004	23.875
3	21.325	22.725	25.825	30.125
4	24.625	28.575	20.575	26.224999999999998
5	23.925	33.225	24.025	18.825
6	18.25	33.800000000000004	26.6	21.349999999999998
7	14.374999999999998	27.05	39.375	19.2
8	17.175	25.05	33.225	24.55
9	18.425	22.95	33.175	25.45
10-14	20.080000000000002	29.57	27.384999999999998	22.965
15-19	20.48	27.99	28.095	23.435
20-24	20.485	28.405	28.34	22.770000000000003
25-29	20.055	28.53	27.97	23.445
30-34	20.01	28.675	27.994999999999997	23.32
35-39	20.395	28.544999999999998	28.349999999999998	22.71
40-44	21.099999999999998	28.78	27.384999999999998	22.735
45-49	20.89	28.205000000000002	28.16	22.745
50-54	20.96	28.46	27.839999999999996	22.74
55-59	20.89	28.060000000000002	28.33	22.720000000000002
60-64	20.755000000000003	27.975	27.939999999999998	23.330000000000002
65-69	20.865000000000002	27.58	28.38	23.175
70-74	21.785	27.735	27.750000000000004	22.73
75-79	20.055	28.565	27.735	23.645
80-84	20.674999999999997	28.715000000000003	27.82	22.79
85-89	21.145	28.904999999999998	27.224999999999998	22.725
90-94	21.17	27.83	27.66	23.34
95-99	20.74	28.325	27.935	23.0
100-104	20.72	27.334999999999997	28.335	23.61
105-109	20.9	28.46	27.455000000000002	23.185
110-114	21.2	27.634999999999998	27.465	23.7
115-119	21.745436359089773	27.591897974493623	28.307076769192296	22.355588897224308
120-124	21.55754514079928	27.084479567848746	28.089831441004353	23.268143850347624
125-129	21.028926033430086	27.925132619357424	28.34551095986388	22.700430387348614
130-134	21.256135430231392	27.221276169488128	28.528498447360512	22.994089952919964
135-139	21.866225431207383	27.366626554352187	27.93321299638989	22.833935018050543
140-144	22.446108235767046	27.355409275915783	27.82272247625747	22.375760012059693
145-149	21.643654149895553	27.375554083660266	27.813725989708054	23.16706577673613
150-151	20.329366177691668	28.037641848879048	27.968447273733737	23.664544699695544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	39.5
2	1.5
3	1.0
4	1.0
5	0.0
6	0.0
7	0.5
8	0.5
9	0.0
10	0.5
11	0.5
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.5
19	0.5
20	0.5
21	1.5
22	1.5
23	0.5
24	1.0
25	4.0
26	3.5
27	5.5
28	9.5
29	9.5
30	12.5
31	16.0
32	25.5
33	29.5
34	39.5
35	55.5
36	64.0
37	87.0
38	121.5
39	147.0
40	180.5
41	210.0
42	231.0
43	251.5
44	258.5
45	260.5
46	240.0
47	243.5
48	245.5
49	206.0
50	166.0
51	146.0
52	128.5
53	98.0
54	71.5
55	64.0
56	70.5
57	54.5
58	35.5
59	33.5
60	23.0
61	12.5
62	10.5
63	7.5
64	8.0
65	7.0
66	4.0
67	3.0
68	2.5
69	3.0
70	2.0
71	0.0
72	0.5
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
114	1.0
115	0.0
116	0.0
117	0.0
118	0.0
119	0.0
120	0.0
121	0.0
122	1.0
123	0.0
124	1.0
125	0.0
126	1.0
127	0.0
128	0.0
129	0.0
130	2.0
131	1.0
132	1.0
133	1.0
134	1.0
135	0.0
136	1.0
137	1.0
138	1.0
139	0.0
140	3.0
141	5.0
142	2.0
143	3.0
144	7.0
145	9.0
146	17.0
147	36.0
148	49.0
149	93.0
150	300.0
151	3463.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.38044914134743	91.2
2	3.4610303830911495	6.550000000000001
3	0.10568031704095111	0.3
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.05284015852047556	1.95
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41	1.0250000000000001	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37	0.9249999999999999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.025	0.0	0.0	0.0	0.0
96-97	0.025	0.0	0.0	0.0	0.0
98-99	0.025	0.0	0.0	0.0	0.0
100-101	0.025	0.0	0.0	0.0	0.0
102-103	0.025	0.0	0.0	0.0	0.0
104-105	0.025	0.0	0.0	0.0	0.0
106-107	0.025	0.0	0.0	0.0	0.0
108-109	0.025	0.0	0.0	0.0	0.0
110-111	0.025	0.0	0.0	0.0	0.0
112-113	0.025	0.0	0.0	0.0	0.0
114-115	0.025	0.0	0.0	0.0	0.0
116-117	0.025	0.0	0.0	0.0	0.0
118-119	0.025	0.0	0.0	0.0	0.0
120-121	0.025	0.0	0.0	0.0	0.0
122-123	0.025	0.0	0.0	0.0	0.0
124-125	0.025	0.0	0.0	0.0	0.0
126-127	0.025	0.0	0.0	0.0	0.0
128-129	0.025	0.0	0.0	0.0	0.0
130-131	0.025	0.0	0.0	0.0	0.0
132-133	0.025	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138-139	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACTA	10	0.0068537686	144.8375	5
AAGATCC	10	0.0068537686	144.8375	9
CTAGAAT	10	0.0068537686	144.8375	1
>>END_MODULE
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561138 spots for SRR12560176.sra
Written 1561138 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
Read 1561136 spots for SRR12560176.sra
Written 1561136 spots for SRR12560176.sra
SRR ids: ['SRR12560176.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_21alqn62
SRR12560176.sra spots: 31222722
blocks: [[1, 1561136], [1561137, 3122272], [3122273, 4683408], [4683409, 6244544], [6244545, 7805680], [7805681, 9366816], [9366817, 10927952], [10927953, 12489088], [12489089, 14050224], [14050225, 15611360], [15611361, 17172496], [17172497, 18733632], [18733633, 20294768], [20294769, 21855904], [21855905, 23417040], [23417041, 24978176], [24978177, 26539312], [26539313, 28100448], [28100449, 29661584], [29661585, 31222722]]
SRR12560176 file size 10304958
SRR12560176 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR12560176 SRR12560176_1.fastq
Input file:	SRR12560176_1.fastq
trimmed:	SRR12560176-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Mon Feb 10 20:57:35 2025 >> started

Mon Feb 10 20:57:56 2025 >> done (20.311s)
31222722 reads processed; of these:
      22 ( 0.00%) short reads filtered out after trimming by size control
       8 ( 0.00%) empty reads filtered out after trimming by size control
31222692 (100.00%) reads available; of these:
    6653 ( 0.02%) trimmed reads available after processing
31216039 (99.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       5	  0.00%
 19	       3	  0.00%
 20	       5	  0.00%
 21	       7	  0.00%
 22	      10	  0.00%
 23	      17	  0.00%
 24	      22	  0.00%
 25	      24	  0.00%
 26	      21	  0.00%
 27	      14	  0.00%
 28	      19	  0.00%
 29	      23	  0.00%
 30	      38	  0.00%
 31	      31	  0.00%
 32	      34	  0.00%
 33	      33	  0.00%
 34	      31	  0.00%
 35	      46	  0.00%
 36	      36	  0.00%
 37	      44	  0.00%
 38	      36	  0.00%
 39	      55	  0.00%
 40	     166	  0.00%
 41	     201	  0.00%
 42	     172	  0.00%
 43	     215	  0.00%
 44	     204	  0.00%
 45	     180	  0.00%
 46	     213	  0.00%
 47	     226	  0.00%
 48	     195	  0.00%
 49	     223	  0.00%
 50	     209	  0.00%
 51	     220	  0.00%
 52	     243	  0.00%
 53	     229	  0.00%
 54	     241	  0.00%
 55	     225	  0.00%
 56	     255	  0.00%
 57	     443	  0.00%
 58	     254	  0.00%
 59	     318	  0.00%
 60	     277	  0.00%
 61	     268	  0.00%
 62	     310	  0.00%
 63	     297	  0.00%
 64	     273	  0.00%
 65	     265	  0.00%
 66	     299	  0.00%
 67	     322	  0.00%
 68	     303	  0.00%
 69	     335	  0.00%
 70	     370	  0.00%
 71	     305	  0.00%
 72	     329	  0.00%
 73	     337	  0.00%
 74	     301	  0.00%
 75	     306	  0.00%
 76	     382	  0.00%
 77	     362	  0.00%
 78	     369	  0.00%
 79	     394	  0.00%
 80	     387	  0.00%
 81	     394	  0.00%
 82	     428	  0.00%
 83	     392	  0.00%
 84	     442	  0.00%
 85	     408	  0.00%
 86	     543	  0.00%
 87	     432	  0.00%
 88	     423	  0.00%
 89	     462	  0.00%
 90	     509	  0.00%
 91	     534	  0.00%
 92	     482	  0.00%
 93	     505	  0.00%
 94	     502	  0.00%
 95	     515	  0.00%
 96	     563	  0.00%
 97	     598	  0.00%
 98	     626	  0.00%
 99	     626	  0.00%
100	     660	  0.00%
101	     677	  0.00%
102	     696	  0.00%
103	     795	  0.00%
104	     823	  0.00%
105	     822	  0.00%
106	     866	  0.00%
107	     867	  0.00%
108	     965	  0.00%
109	     991	  0.00%
110	    1041	  0.00%
111	    1049	  0.00%
112	    1037	  0.00%
113	    1168	  0.00%
114	    1235	  0.00%
115	    1260	  0.00%
116	    1282	  0.00%
117	    1307	  0.00%
118	    1430	  0.00%
119	    1513	  0.00%
120	    1558	  0.00%
121	    1581	  0.01%
122	    1686	  0.01%
123	    1775	  0.01%
124	    1947	  0.01%
125	    1997	  0.01%
126	    2231	  0.01%
127	    2432	  0.01%
128	    2536	  0.01%
129	    2875	  0.01%
130	    2927	  0.01%
131	    3498	  0.01%
132	    3898	  0.01%
133	    4378	  0.01%
134	    4990	  0.02%
135	    5913	  0.02%
136	    7053	  0.02%
137	    7950	  0.03%
138	    9294	  0.03%
139	   11457	  0.04%
140	   13085	  0.04%
141	   16492	  0.05%
142	   21396	  0.07%
143	   30983	  0.10%
144	   46485	  0.15%
145	   70945	  0.23%
146	   97139	  0.31%
147	  141821	  0.45%
148	  280309	  0.90%
149	  536726	  1.72%
150	 2586285	  8.28%
151	27257205	 87.30%
31222692 reads passed initial QC


criterion=sequence-density
sequence-density=0.14
sequence-density-rank=1
fanout-score=7.54
fanout-score-rank=15
prefix-density=0.25
prefix-fanout=4.4
sequence=TCCTTGTCCTGGATCTTGGCCTTCAC


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=23
fanout-score=59.29
fanout-score-rank=1
prefix-density=0.34
prefix-fanout=11.9
sequence=ATCTCAGCAAACTT
                                 Started job on |	Feb 10 20:58:21
                             Started mapping on |	Feb 10 20:58:21
                                    Finished on |	Feb 10 20:59:50
       Mapping speed, Million of reads per hour |	1262.94

                          Number of input reads |	31222692
                      Average input read length |	150
                                    UNIQUE READS:
                   Uniquely mapped reads number |	28067083
                        Uniquely mapped reads % |	89.89%
                          Average mapped length |	150.25
                       Number of splices: Total |	12696028
            Number of splices: Annotated (sjdb) |	12491241
                       Number of splices: GT/AG |	12473968
                       Number of splices: GC/AG |	173896
                       Number of splices: AT/AC |	11872
               Number of splices: Non-canonical |	36292
                      Mismatch rate per base, % |	0.44%
                         Deletion rate per base |	0.03%
                        Deletion average length |	2.71
                        Insertion rate per base |	0.02%
                       Insertion average length |	2.28
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	923998
             % of reads mapped to multiple loci |	2.96%
        Number of reads mapped to too many loci |	381476
             % of reads mapped to too many loci |	1.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.25%
                     % of reads unmapped: other |	1.68%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2231611	2231611	2231611
N_multimapping	923998	923998	923998
N_noFeature	682352	27822865	798195
N_ambiguous	216189	2448	85639
UnstrandedReadsAssigned:27168542 PositiveStrandReadsAssigned:241770 NegativeStrandReadsAssigned:27183249
Dataset is classified negative stranded
MeadianReadLen=151 20thPercentileLength=151 echo kmer=147
SRR12560176 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in single-end mode
[quant] will process file 1: SRR12560176-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,222,692 reads, 28,269,061 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,261 rounds

  52401 SRR12560176.ke.tsv
  34699 SRR12560176.se.tsv
  87100 total
==> SRR12560176.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1919	2050	58.8624
Potri.005G024800.1.v4.1	1035	936	213	12.539
Potri.004G059700.1.v4.1	961	862	245	15.6609
Potri.007G009000.2.v4.1	1416	1317	0	0
Potri.003G141000.2.v4.1	2943	2844	705.102	13.6609
Potri.016G087400.1.v4.1	270	171	349	112.457
Potri.015G069301.1.v4.1	564	465	0	0
Potri.010G195200.1.v4.1	1773	1674	181	5.95775
Potri.012G127500.1.v4.1	977	878	5121	321.38

==> SRR12560176.se.tsv <==
Potri.001G166300.v4.1	0
Potri.001G448400.v4.1	19
Potri.001G233950.v4.1	0
Potri.001G122700.v4.1	119
Potri.001G212900.v4.1	0
Potri.001G182400.v4.1	6
Potri.001G256600.v4.1	0
Potri.001G040500.v4.1	1
Potri.001G416900.v4.1	1267
Potri.001G452600.v4.1	781
SRR12560176 completed mapping pipeline successfully
