Starting /dee2/code/volunteer_pipeline.sh SRR12560178
    current disk space = 3056842186752
    free memory = 1442845016 
SRR12560178 SRAfilesize
b5a1ea9e182d727cbcda1072dd868cd0  SRR12560178.sra
SRR12560178.sra file validated
SRR12560178 is single end
SRR12560178 is conventional basespace
SRR12560178 read1 length is 96-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR12560178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	96-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88625	32.0	32.0	32.0	32.0	32.0
2	31.24875	32.0	32.0	32.0	32.0	32.0
3	34.59875	37.0	32.0	37.0	32.0	37.0
4	36.02425	37.0	37.0	37.0	32.0	37.0
5	36.059	37.0	37.0	37.0	32.0	37.0
6	39.1955	41.0	37.0	41.0	37.0	41.0
7	39.54425	41.0	41.0	41.0	37.0	41.0
8	39.5345	41.0	41.0	41.0	37.0	41.0
9	39.2015	41.0	41.0	41.0	37.0	41.0
10-14	39.48	41.0	41.0	41.0	37.0	41.0
15-19	39.5713	41.0	41.0	41.0	37.0	41.0
20-24	39.54765	41.0	41.0	41.0	37.0	41.0
25-29	39.1047	41.0	41.0	41.0	37.0	41.0
30-34	38.9937	41.0	41.0	41.0	35.0	41.0
35-39	39.367000000000004	41.0	41.0	41.0	37.0	41.0
40-44	39.28975	41.0	41.0	41.0	37.0	41.0
45-49	39.317400000000006	41.0	41.0	41.0	37.0	41.0
50-54	39.096999999999994	41.0	41.0	41.0	36.0	41.0
55-59	39.09375	41.0	41.0	41.0	37.0	41.0
60-64	38.72425	41.0	41.0	41.0	33.0	41.0
65-69	38.71585	41.0	39.4	41.0	33.0	41.0
70-74	38.86095	41.0	41.0	41.0	33.0	41.0
75-79	38.90355	41.0	39.4	41.0	35.0	41.0
80-84	39.11579999999999	41.0	41.0	41.0	37.0	41.0
85-89	39.120650000000005	41.0	41.0	41.0	36.0	41.0
90-94	39.3267	41.0	41.0	41.0	37.0	41.0
95-99	39.11191336584146	41.0	41.0	41.0	37.0	41.0
100-104	39.01225337600033	41.0	41.0	41.0	35.0	41.0
105-109	38.88699349674838	41.0	41.0	41.0	33.0	41.0
110-114	38.700200100050026	41.0	41.0	41.0	32.0	41.0
115-119	38.39029440963337	41.0	37.8	41.0	32.0	41.0
120-124	38.34182728410512	41.0	37.0	41.0	32.0	41.0
125-129	38.040930678209214	41.0	37.0	41.0	32.0	41.0
130-134	37.45659904833458	41.0	37.0	41.0	28.0	41.0
135-139	36.93844227397947	41.0	37.0	41.0	27.0	41.0
140-144	36.744563816919836	41.0	37.0	41.0	27.0	41.0
145-149	36.78998932263174	41.0	37.0	41.0	28.0	41.0
150-151	37.158632818653544	41.0	34.5	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	3.0
24	5.0
25	8.0
26	5.0
27	10.0
28	17.0
29	19.0
30	43.0
31	50.0
32	70.0
33	88.0
34	120.0
35	159.0
36	228.0
37	294.0
38	394.0
39	748.0
40	1737.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.474999999999998	11.774999999999999	16.35	44.4
2	24.099999999999998	17.549999999999997	36.449999999999996	21.9
3	21.25	23.474999999999998	23.7	31.574999999999996
4	25.0	29.975	20.575	24.45
5	22.900000000000002	33.675	23.125	20.3
6	19.175	34.175	26.0	20.65
7	13.375	26.125	40.550000000000004	19.950000000000003
8	17.0	22.25	34.35	26.400000000000002
9	19.075	21.825	32.975	26.125
10-14	19.72	29.565	27.24	23.474999999999998
15-19	19.86	28.244999999999997	27.915	23.98
20-24	20.380000000000003	28.67	27.355	23.595
25-29	20.39	28.660000000000004	27.075	23.875
30-34	20.03	28.310000000000002	27.955000000000002	23.705000000000002
35-39	20.580000000000002	28.405	27.650000000000002	23.365
40-44	20.3	28.655	27.495000000000005	23.549999999999997
45-49	20.605	28.04	27.805000000000003	23.549999999999997
50-54	20.47	28.49	27.615000000000002	23.425
55-59	19.950000000000003	28.535	27.515	24.0
60-64	20.25	28.335	27.810000000000002	23.605
65-69	20.635	28.005000000000003	27.375	23.985
70-74	20.93	28.52	27.12	23.43
75-79	20.26	27.655	27.96	24.125
80-84	20.549999999999997	27.625	27.884999999999998	23.94
85-89	20.974999999999998	27.715	27.29	24.02
90-94	20.695	28.075	27.525	23.705000000000002
95-99	20.878131719757963	27.614142121318196	27.509126368955343	23.998599789968495
100-104	20.954429493271974	27.537391826321844	28.107648441798812	23.400530238607374
105-109	20.770385192596297	27.808904452226113	27.64382191095548	23.77688844422211
110-114	20.70535267633817	27.923961980990498	27.538769384692348	23.83191595797899
115-119	21.031825460368296	27.527021617293833	27.762209767814248	23.67894315452362
120-124	20.58072590738423	27.63454317897372	27.639549436795996	24.14518147684606
125-129	20.761141712568854	27.74161241862794	27.46119178768152	24.036054081121684
130-134	20.500876533934385	27.613323315802656	27.988980716253444	23.896819434009515
135-139	21.182068620085147	27.39794640621087	27.643375907838717	23.776609065865266
140-144	21.394882087305568	27.52634219769192	27.395885599598596	23.68289011540391
145-149	20.9715940850653	27.46074495655267	27.435337161441133	24.132323796940902
150-151	20.970608339029393	27.436773752563226	27.34107997265892	24.251537935748463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	3.0
2	0.5
3	1.0
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	1.0
13	1.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	1.5
24	3.0
25	5.0
26	7.0
27	8.0
28	8.0
29	11.0
30	17.5
31	30.0
32	31.5
33	35.5
34	48.5
35	59.0
36	67.0
37	88.5
38	121.0
39	147.0
40	194.0
41	208.0
42	216.5
43	253.0
44	277.0
45	279.5
46	272.5
47	258.5
48	217.5
49	194.5
50	190.5
51	156.5
52	123.5
53	103.0
54	80.5
55	63.5
56	52.0
57	45.5
58	31.0
59	20.5
60	16.0
61	12.5
62	8.5
63	3.5
64	3.5
65	4.5
66	2.5
67	3.0
68	2.0
69	1.0
70	0.5
71	0.0
72	0.5
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
96-97	1.0
98-99	0.0
100-101	1.0
102-103	0.0
104-105	0.0
106-107	0.0
108-109	0.0
110-111	0.0
112-113	0.0
114-115	1.0
116-117	1.0
118-119	1.0
120-121	0.0
122-123	0.0
124-125	0.0
126-127	2.0
128-129	0.0
130-131	0.0
132-133	0.0
134-135	0.0
136-137	0.0
138-139	3.0
140-141	3.0
142-143	6.0
144-145	13.0
146-147	55.0
148-149	110.0
150-151	3803.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.1
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.17585848074923	92.425
2	3.6680541103017688	7.049999999999999
3	0.1300728407908429	0.375
4	0.0	0.0
5	0.0	0.0
6	0.026014568158168577	0.15
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCATG	10	0.0068519996	144.85	8
TTCATGG	10	0.0068519996	144.85	9
>>END_MODULE
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276826 spots for SRR12560178.sra
Written 2276826 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
Read 2276814 spots for SRR12560178.sra
Written 2276814 spots for SRR12560178.sra
SRR ids: ['SRR12560178.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_x9c88rub
SRR12560178.sra spots: 45536292
blocks: [[1, 2276814], [2276815, 4553628], [4553629, 6830442], [6830443, 9107256], [9107257, 11384070], [11384071, 13660884], [13660885, 15937698], [15937699, 18214512], [18214513, 20491326], [20491327, 22768140], [22768141, 25044954], [25044955, 27321768], [27321769, 29598582], [29598583, 31875396], [31875397, 34152210], [34152211, 36429024], [36429025, 38705838], [38705839, 40982652], [40982653, 43259466], [43259467, 45536292]]
SRR12560178 file size 15017835
SRR12560178 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR12560178 SRR12560178_1.fastq
Input file:	SRR12560178_1.fastq
trimmed:	SRR12560178-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Mon Feb 10 21:15:34 2025 >> started

Mon Feb 10 21:16:13 2025 >> done (39.274s)
45536292 reads processed; of these:
       7 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
45536285 (100.00%) reads available; of these:
    5247 ( 0.01%) trimmed reads available after processing
45531038 (99.99%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       2	  0.00%
 19	       3	  0.00%
 20	       4	  0.00%
 21	      14	  0.00%
 22	       8	  0.00%
 23	      10	  0.00%
 24	      14	  0.00%
 25	      21	  0.00%
 26	       9	  0.00%
 27	      18	  0.00%
 28	      20	  0.00%
 29	      39	  0.00%
 30	      41	  0.00%
 31	      21	  0.00%
 32	      40	  0.00%
 33	      28	  0.00%
 34	      38	  0.00%
 35	      33	  0.00%
 36	      32	  0.00%
 37	      22	  0.00%
 38	      43	  0.00%
 39	      44	  0.00%
 40	     138	  0.00%
 41	     131	  0.00%
 42	     141	  0.00%
 43	     151	  0.00%
 44	     241	  0.00%
 45	     142	  0.00%
 46	     162	  0.00%
 47	     167	  0.00%
 48	     143	  0.00%
 49	     191	  0.00%
 50	     160	  0.00%
 51	     170	  0.00%
 52	     160	  0.00%
 53	     205	  0.00%
 54	     216	  0.00%
 55	     220	  0.00%
 56	     222	  0.00%
 57	     398	  0.00%
 58	     240	  0.00%
 59	     244	  0.00%
 60	     256	  0.00%
 61	     254	  0.00%
 62	     267	  0.00%
 63	     261	  0.00%
 64	     230	  0.00%
 65	     290	  0.00%
 66	     310	  0.00%
 67	     287	  0.00%
 68	     304	  0.00%
 69	     303	  0.00%
 70	     334	  0.00%
 71	     293	  0.00%
 72	     308	  0.00%
 73	     350	  0.00%
 74	     356	  0.00%
 75	     362	  0.00%
 76	     425	  0.00%
 77	     416	  0.00%
 78	     454	  0.00%
 79	     535	  0.00%
 80	     599	  0.00%
 81	     615	  0.00%
 82	     667	  0.00%
 83	     783	  0.00%
 84	     764	  0.00%
 85	     817	  0.00%
 86	     997	  0.00%
 87	     945	  0.00%
 88	    1055	  0.00%
 89	    1176	  0.00%
 90	    1208	  0.00%
 91	    1299	  0.00%
 92	    1338	  0.00%
 93	    1240	  0.00%
 94	    1351	  0.00%
 95	    1426	  0.00%
 96	    1499	  0.00%
 97	    1680	  0.00%
 98	    1865	  0.00%
 99	    1874	  0.00%
100	    2023	  0.00%
101	    2178	  0.00%
102	    2136	  0.00%
103	    2441	  0.01%
104	    2617	  0.01%
105	    2755	  0.01%
106	    2735	  0.01%
107	    2977	  0.01%
108	    3017	  0.01%
109	    3175	  0.01%
110	    3742	  0.01%
111	    3943	  0.01%
112	    4088	  0.01%
113	    4351	  0.01%
114	    4604	  0.01%
115	    4868	  0.01%
116	    5050	  0.01%
117	    5466	  0.01%
118	    5677	  0.01%
119	    5969	  0.01%
120	    6260	  0.01%
121	    7129	  0.02%
122	    7808	  0.02%
123	    8263	  0.02%
124	    8854	  0.02%
125	    9239	  0.02%
126	    9877	  0.02%
127	   10409	  0.02%
128	   11244	  0.02%
129	   12206	  0.03%
130	   13403	  0.03%
131	   14724	  0.03%
132	   15842	  0.03%
133	   17546	  0.04%
134	   19294	  0.04%
135	   21155	  0.05%
136	   23823	  0.05%
137	   27173	  0.06%
138	   29933	  0.07%
139	   35643	  0.08%
140	   39336	  0.09%
141	   47701	  0.10%
142	   58527	  0.13%
143	   71739	  0.16%
144	   95794	  0.21%
145	  128514	  0.28%
146	  173459	  0.38%
147	  257480	  0.57%
148	  455218	  1.00%
149	  889002	  1.95%
150	 4475252	  9.83%
151	38426487	 84.39%
45536285 reads passed initial QC


criterion=sequence-density
sequence-density=0.13
sequence-density-rank=1
fanout-score=3.99
fanout-score-rank=29
prefix-density=0.20
prefix-fanout=2.6
sequence=ACCAAGTGGAGG


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=28
fanout-score=317.89
fanout-score-rank=1
prefix-density=0.69
prefix-fanout=30.5
sequence=TCTTCTTCTTCCT
                                 Started job on |	Feb 10 21:16:36
                             Started mapping on |	Feb 10 21:16:36
                                    Finished on |	Feb 10 21:18:27
       Mapping speed, Million of reads per hour |	1476.85

                          Number of input reads |	45536285
                      Average input read length |	150
                                    UNIQUE READS:
                   Uniquely mapped reads number |	42479726
                        Uniquely mapped reads % |	93.29%
                          Average mapped length |	150.11
                       Number of splices: Total |	20491373
            Number of splices: Annotated (sjdb) |	20186090
                       Number of splices: GT/AG |	20134626
                       Number of splices: GC/AG |	288414
                       Number of splices: AT/AC |	16298
               Number of splices: Non-canonical |	52035
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.03%
                        Deletion average length |	2.64
                        Insertion rate per base |	0.02%
                       Insertion average length |	2.31
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1304012
             % of reads mapped to multiple loci |	2.86%
        Number of reads mapped to too many loci |	324159
             % of reads mapped to too many loci |	0.71%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.96%
                     % of reads unmapped: other |	0.18%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1752547	1752547	1752547
N_multimapping	1304012	1304012	1304012
N_noFeature	1052646	42155421	1203177
N_ambiguous	305847	2508	130083
UnstrandedReadsAssigned:41121233 PositiveStrandReadsAssigned:321797 NegativeStrandReadsAssigned:41146466
Dataset is classified negative stranded
MeadianReadLen=151 20thPercentileLength=151 echo kmer=147
SRR12560178 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in single-end mode
[quant] will process file 1: SRR12560178-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 45,536,285 reads, 41,812,783 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,266 rounds

  52401 SRR12560178.ke.tsv
  34699 SRR12560178.se.tsv
  87100 total
==> SRR12560178.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1919	3404	68.0336
Potri.005G024800.1.v4.1	1035	936	491	20.1194
Potri.004G059700.1.v4.1	961	862	61	2.71413
Potri.007G009000.2.v4.1	1416	1317	1	0.0291221
Potri.003G141000.2.v4.1	2943	2844	1598.27	21.5541
Potri.016G087400.1.v4.1	270	171	605	135.696
Potri.015G069301.1.v4.1	564	465	0	0
Potri.010G195200.1.v4.1	1773	1674	280	6.41522
Potri.012G127500.1.v4.1	977	878	8069	352.48

==> SRR12560178.se.tsv <==
Potri.001G166300.v4.1	1
Potri.001G448400.v4.1	55
Potri.001G233950.v4.1	0
Potri.001G122700.v4.1	196
Potri.001G212900.v4.1	0
Potri.001G182400.v4.1	6
Potri.001G256600.v4.1	0
Potri.001G040500.v4.1	6
Potri.001G416900.v4.1	691
Potri.001G452600.v4.1	335
SRR12560178 completed mapping pipeline successfully
