Starting /dee2/code/volunteer_pipeline.sh SRR12768913 current disk space = 3056992915456 free memory = 1467228896 SRR12768913 SRAfilesize ed4615156719c201babdd505638cf4c7 SRR12768913.sra SRR12768913.sra file validated SRR12768913 is paired end SRR12768913 is conventional basespace SRR12768913 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR12768913_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7205 37.0 37.0 37.0 37.0 37.0 2 36.361 37.0 37.0 37.0 37.0 37.0 3 36.4265 37.0 37.0 37.0 37.0 37.0 4 35.9295 37.0 37.0 37.0 37.0 37.0 5 35.568 37.0 37.0 37.0 37.0 37.0 6 35.7185 37.0 37.0 37.0 37.0 37.0 7 35.404 37.0 37.0 37.0 37.0 37.0 8 35.5945 37.0 37.0 37.0 37.0 37.0 9 35.3055 37.0 37.0 37.0 37.0 37.0 10-14 35.2996 37.0 37.0 37.0 29.8 37.0 15-19 35.974199999999996 37.0 37.0 37.0 37.0 37.0 20-24 35.7601 37.0 37.0 37.0 37.0 37.0 25-29 35.7667 37.0 37.0 37.0 37.0 37.0 30-34 35.43300000000001 37.0 37.0 37.0 32.2 37.0 35-39 35.5789 37.0 37.0 37.0 34.6 37.0 40-44 35.8754 37.0 37.0 37.0 37.0 37.0 45-49 35.762299999999996 37.0 37.0 37.0 37.0 37.0 50-54 35.785900000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.7822 37.0 37.0 37.0 37.0 37.0 60-64 35.756499999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.57299999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.4604 37.0 37.0 37.0 37.0 37.0 75-79 35.0301 37.0 37.0 37.0 27.4 37.0 80-84 34.119299999999996 37.0 37.0 37.0 25.0 37.0 85-89 33.2772 37.0 37.0 37.0 16.6 37.0 90-94 32.1976 37.0 34.6 37.0 11.0 37.0 95-99 30.7617 37.0 25.0 37.0 11.0 37.0 100-104 29.995600000000003 37.0 25.0 37.0 11.0 37.0 105-109 29.3645 37.0 25.0 37.0 11.0 37.0 110-114 29.485200000000003 37.0 25.0 37.0 11.0 37.0 115-119 29.7832 37.0 25.0 37.0 11.0 37.0 120-124 30.6427 37.0 25.0 37.0 11.0 37.0 125-129 31.261599999999998 37.0 25.0 37.0 11.0 37.0 130-134 32.0922 37.0 34.6 37.0 11.0 37.0 135-139 32.443799999999996 37.0 37.0 37.0 11.0 37.0 140-144 33.0976 37.0 37.0 37.0 11.0 37.0 145-149 33.543099999999995 37.0 37.0 37.0 19.4 37.0 150 33.786 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 2.0 22 6.0 23 12.0 24 16.0 25 21.0 26 36.0 27 45.0 28 73.0 29 87.0 30 135.0 31 177.0 32 331.0 33 787.0 34 1308.0 35 394.0 36 543.0 37 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.6258129064532265 94.89744872436218 2.076038019009505 1.4007003501750876 2 1.55 2.675 5.45 90.325 3 11.75 71.45 9.35 7.449999999999999 4 19.475 35.699999999999996 31.4 13.425 5 32.5 24.075 29.7 13.725000000000001 6 28.975 24.224999999999998 31.525 15.275 7 23.625 26.825 32.45 17.1 8 25.05 24.725 32.074999999999996 18.15 9 28.499999999999996 24.575 31.1 15.825 10-14 25.885 24.895 31.324999999999996 17.895 15-19 25.629999999999995 25.285000000000004 31.09 17.995 20-24 25.185000000000002 25.014999999999997 31.44 18.360000000000003 25-29 24.18 25.91 31.380000000000003 18.529999999999998 30-34 24.64 27.355 29.87 18.135 35-39 25.16 27.560000000000002 29.354999999999997 17.925 40-44 25.869999999999997 29.134999999999998 27.13 17.865000000000002 45-49 25.97 29.555 25.615 18.86 50-54 25.685000000000002 29.995 24.215 20.105 55-59 24.795 30.615 23.305 21.285 60-64 24.02 30.075000000000003 22.725 23.18 65-69 21.575 28.865000000000002 24.135 25.424999999999997 70-74 19.755 28.355000000000004 24.88 27.01 75-79 18.89 27.155 26.064999999999998 27.889999999999997 80-84 20.035 25.8 26.31 27.855 85-89 18.655 23.915 28.375 29.054999999999996 90-94 19.66 21.740000000000002 30.035 28.565 95-99 21.825 21.775 29.535 26.865 100-104 25.635 21.465 28.23 24.67 105-109 30.880000000000003 20.62 26.334999999999997 22.165000000000003 110-114 37.445 18.78 24.335 19.439999999999998 115-119 43.79 18.34 20.935000000000002 16.935 120-124 51.525 15.584999999999999 18.575 14.315 125-129 57.550000000000004 13.755 15.709999999999999 12.985 130-134 61.46 12.754999999999999 14.885000000000002 10.9 135-139 66.535 10.93 12.8 9.735000000000001 140-144 70.02000000000001 10.27 11.205 8.505 145-149 73.57000000000001 8.790000000000001 9.8 7.84 150 75.325 9.3 8.975 6.4 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 1.5 8 0.0 9 0.5 10 1.5 11 1.0 12 1.0 13 1.5 14 1.0 15 0.5 16 0.0 17 2.0 18 4.0 19 3.5 20 3.0 21 3.5 22 2.5 23 2.5 24 4.0 25 3.5 26 4.5 27 8.5 28 9.0 29 9.5 30 14.5 31 17.0 32 16.5 33 21.0 34 32.5 35 40.0 36 57.5 37 77.0 38 90.5 39 107.0 40 125.0 41 149.0 42 179.0 43 195.5 44 217.5 45 275.5 46 307.0 47 312.5 48 321.5 49 318.0 50 275.0 51 218.5 52 191.0 53 151.5 54 97.5 55 49.5 56 26.0 57 16.0 58 7.0 59 7.5 60 5.0 61 4.0 62 3.0 63 1.0 64 0.5 65 1.0 66 1.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.55 #Duplication Level Percentage of deduplicated Percentage of total 1 90.32729398012857 77.275 2 7.364114552893045 12.6 3 1.1981297486849796 3.075 4 0.2630040911747516 0.8999999999999999 5 0.3798947983635301 1.625 6 0.0 0.0 7 0.11689070718877849 0.7000000000000001 8 0.08766803039158387 0.6 9 0.029222676797194622 0.22499999999999998 >10 0.23378141437755698 3.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACATGTTAGTGCTCCATGATCTTGAGAAGGAAACCTTGTCATTCCTTCCA 25 0.625 No Hit ACGTGTTGCTGTTGATATTTAATTTGGTAATTTGATGTCAATTTTCTGAT 17 0.42500000000000004 No Hit ACAAGTAGGAGCTCGAATTTCCCCGATCGTTCAAAGATCGGAAGAGCACA 15 0.375 No Hit ACAACGATCAAGAGCGACTGTGCTATTCCTGCAATTCTTGCAAAGCTGGT 14 0.35000000000000003 No Hit ACGTGTTCTTTTGAAGATTGTTGTTCGATTGTTACCTGAGAAAAGAAGAT 14 0.35000000000000003 No Hit ACAAACTATGTGACCAGATCTCTGATGCAGTGCTCGATGCCTGCCTTGAG 13 0.325 No Hit ACGTGTGATCTTTGGTATTGTAATAAAAAAAATAGATCGGAAGAGCACAC 11 0.27499999999999997 No Hit ACTATGCGACCAGATCTCTGATGCGGTGCTCGATGCTTGCCTTGAGCAGG 11 0.27499999999999997 No Hit ACATTGGATTTGTTTCTGACGACGTTGGTCTTGATGCTGAGATCGGAAGA 9 0.22499999999999998 No Hit ACTATGTGACCAGATCTCTGATGCAGTGCTCGATGCCTGCCTTGAGCAGG 8 0.2 No Hit ACAGAATAACTTCTCTGAAGATGCTGAAACCGATATGGTCGATGAGATCG 8 0.2 No Hit ACATCCCAATTTCAGACTGTGAGTAACCCTGTAAGGAGATCGGAAGAGCA 8 0.2 No Hit ACAGACGTGGTTCCGAGGATGTAAGCAAAGATCGGAAGAGCACACGTCTG 7 0.17500000000000002 Illumina Multiplexing PCR Primer 2.01 (100% over 22bp) ACAAAACTGTATGGAGTCTATTAAACTTCTATGCCTACGTGAAAAACCAC 7 0.17500000000000002 No Hit ACAATATGAGTCGGTCAGGTTCTTTTCATCATTTAGAGAAAGACTGTGGA 7 0.17500000000000002 No Hit ACAAGGCCTATGGGATGACTGCATTTGAATATCATGGCACGGATCCAAGA 7 0.17500000000000002 No Hit ACAAAACCTTTGGGAATTGGAATATGATCCTCGTTGTAGTTTCTCTCAAG 5 0.125 No Hit ACGTGTCATTTATCTGGTTTGATCAATTCTGAAGCTATTATTTGTTTCCA 5 0.125 No Hit ACATCTTATGGGTTAACCCTGATTGTGGACTCAAGACTCGCAAGTAGATC 5 0.125 No Hit ACAATGTTGGTTTCAATGTTAAGAATGTTGCTGTCAAGGATCTCAAAGAT 5 0.125 No Hit ACAAACGTGTTGCTGTTGATATTTAATTTGGTAATTTGATGTCAATTTTC 5 0.125 No Hit ACAAGGAGATCCTTAAGATTGTGAAGGAGAGCTTTGACTTTAGGCCTGGA 5 0.125 No Hit ACATATGGATCGGTGGTTCTGGTATGGACCTTAGAAGCAAAGCCAGGAGA 5 0.125 No Hit ACCAAGGGAGCTTTCGTGCAGCCTGCTTTGTATGAGGCTTTTGGATTGAC 5 0.125 No Hit ACCATGTCTGTTGGTACGTGTGATCTTTGGTATTGTAATAAAAAAAATAG 5 0.125 No Hit ACAGAATAACTACTCTGAAGAGGCTGAAACCGATATGGTTGATGAGATCG 5 0.125 No Hit ACAAACTATGCGACCAGATCTCTGATGCGGTGCTCGATGCTTGCCTTGAG 5 0.125 No Hit ACATGTGTGGTCTAGTTTTTGACGATCTTTTGGCAGATCGGAAGAGCACA 5 0.125 No Hit ACAATGAAGGTTGTAGCCGCTTACTTGCTCGCCGTTCTCGGTGGCAAGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.075 0.0 0.0 0.0 0.0 6 0.075 0.0 0.0 0.0 0.0 7 0.075 0.0 0.0 0.0 0.0 8 0.075 0.0 0.0 0.0 0.0 9 0.1 0.0 0.0 0.0 0.0 10-11 0.2 0.0 0.0 0.0 0.0 12-13 0.2625 0.0 0.0 0.0 0.0 14-15 0.3 0.0 0.0 0.0 0.0 16-17 0.375 0.0 0.0 0.0 0.0 18-19 0.4375 0.0 0.0 0.0 0.0 20-21 0.5375 0.0 0.0 0.0 0.0 22-23 0.8500000000000001 0.0 0.0 0.0 0.0 24-25 1.2125 0.0 0.0 0.0 0.0 26-27 1.6 0.0 0.0 0.0 0.0 28-29 2.4375 0.0 0.0 0.0 0.0 30-31 3.7750000000000004 0.0 0.0 0.0 0.0 32-33 5.525 0.0 0.0 0.0 0.0 34-35 8.125 0.0 0.0 0.0 0.0 36-37 10.925 0.0 0.0 0.0 0.0 38-39 13.825 0.0 0.0 0.0 0.0 40-41 17.0625 0.0 0.0 0.0 0.0 42-43 20.2875 0.0 0.0 0.0 0.0 44-45 23.3125 0.0 0.0 0.0 0.0 46-47 26.612499999999997 0.0 0.0 0.0 0.0 48-49 30.25 0.0 0.0 0.0 0.0 50-51 33.675 0.0 0.0 0.0 0.0 52-53 37.2 0.0 0.0 0.0 0.0 54-55 40.3875 0.0 0.0 0.0 0.0 56-57 43.075 0.0 0.0 0.0 0.0 58-59 45.525000000000006 0.0 0.0 0.0 0.0 60-61 48.0625 0.0 0.0 0.0 0.0 62-63 50.375 0.0 0.0 0.0 0.0 64-65 52.4625 0.0 0.0 0.0 0.0 66-67 54.8 0.0 0.0 0.0 0.0 68-69 56.6375 0.0 0.0 0.0 0.0 70-71 58.4375 0.0 0.0 0.0 0.0 72-73 60.125 0.0 0.0 0.0 0.0 74-75 62.0625 0.0 0.0 0.0 0.0 76-77 63.7625 0.0 0.0 0.0 0.0 78-79 64.9375 0.0 0.0 0.0 0.0 80-81 65.86250000000001 0.0 0.0 0.0 0.0 82-83 67.0375 0.0 0.0 0.0 0.0 84-85 68.2125 0.0 0.0 0.0 0.0 86-87 69.0875 0.0 0.0 0.0 0.0 88-89 69.8125 0.0 0.0 0.0 0.0 90-91 70.67500000000001 0.0 0.0 0.0 0.0 92-93 71.6125 0.0 0.0 0.0 0.0 94-95 72.4375 0.0 0.0 0.0 0.0 96-97 73.2125 0.0 0.0 0.0 0.0 98-99 74.01249999999999 0.0 0.0 0.0 0.0 100-101 74.86250000000001 0.0 0.0 0.0 0.0 102-103 75.73750000000001 0.0 0.0 0.0 0.0 104-105 76.2625 0.0 0.0 0.0 0.0 106-107 76.9375 0.0 0.0 0.0 0.0 108-109 77.475 0.0 0.0 0.0 0.0 110-111 77.9625 0.0 0.0 0.0 0.0 112-113 78.5875 0.0 0.0 0.0 0.0 114-115 79.1 0.0 0.0 0.0 0.0 116-117 79.5 0.0 0.0 0.0 0.0 118-119 79.8625 0.0 0.0 0.0 0.0 120-121 80.0625 0.0 0.0 0.0 0.0 122-123 80.42500000000001 0.0 0.0 0.0 0.0 124-125 80.9 0.0 0.0 0.0 0.0 126-127 81.4 0.0 0.0 0.0 0.0 128-129 81.725 0.0 0.0 0.0 0.0 130-131 82.01249999999999 0.0 0.0 0.0 0.0 132-133 82.3 0.0 0.0 0.0 0.0 134-135 82.55 0.0 0.0 0.0 0.0 136-137 82.8375 0.0 0.0 0.0 0.0 138 83.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGCCT 10 0.006973645 144.0 1 ACAGCCA 10 0.006973645 144.0 1 CTCTGCG 10 0.006973645 144.0 9 ACAGAGA 10 0.006973645 144.0 1 AACGATC 10 0.006973645 144.0 3 ACACTAG 10 0.006973645 144.0 1 ACATTTC 10 0.006973645 144.0 1 ACATATG 10 0.006973645 144.0 1 GCTGATA 10 0.006973645 144.0 9 ATATGGA 10 0.006973645 144.0 3 ACCCAGT 10 0.006973645 144.0 1 ACGATCA 10 0.006973645 144.0 4 GCTCTGC 10 0.006973645 144.0 8 ACAACGA 10 0.006973645 144.0 1 CAACGAT 10 0.006973645 144.0 2 CGATCAA 10 0.006973645 144.0 5 AAAGGGG 150 0.0024146368 8.639999 120-124 CTGCTTG 170 0.0074696713 7.6235294 110-114 TCTTCTG 195 0.0030106967 7.3846154 105-109 GGGGGGG 6670 0.0 5.116642 140-144 >>END_MODULE SRR12768913 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR12768913_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.442 37.0 37.0 37.0 37.0 37.0 2 36.155 37.0 37.0 37.0 37.0 37.0 3 36.2565 37.0 37.0 37.0 37.0 37.0 4 36.308 37.0 37.0 37.0 37.0 37.0 5 36.5315 37.0 37.0 37.0 37.0 37.0 6 36.1775 37.0 37.0 37.0 37.0 37.0 7 36.567 37.0 37.0 37.0 37.0 37.0 8 36.5515 37.0 37.0 37.0 37.0 37.0 9 36.4065 37.0 37.0 37.0 37.0 37.0 10-14 36.4649 37.0 37.0 37.0 37.0 37.0 15-19 36.4753 37.0 37.0 37.0 37.0 37.0 20-24 36.378499999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.373 37.0 37.0 37.0 37.0 37.0 30-34 36.199600000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.1386 37.0 37.0 37.0 37.0 37.0 40-44 36.1826 37.0 37.0 37.0 37.0 37.0 45-49 36.1991 37.0 37.0 37.0 37.0 37.0 50-54 36.1426 37.0 37.0 37.0 37.0 37.0 55-59 36.099900000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.1046 37.0 37.0 37.0 37.0 37.0 65-69 36.1302 37.0 37.0 37.0 37.0 37.0 70-74 35.994600000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.7283 37.0 37.0 37.0 37.0 37.0 80-84 35.329899999999995 37.0 37.0 37.0 34.6 37.0 85-89 34.876400000000004 37.0 37.0 37.0 27.4 37.0 90-94 34.1828 37.0 37.0 37.0 25.0 37.0 95-99 33.4872 37.0 37.0 37.0 19.4 37.0 100-104 32.992599999999996 37.0 37.0 37.0 11.0 37.0 105-109 32.6605 37.0 37.0 37.0 11.0 37.0 110-114 32.387299999999996 37.0 37.0 37.0 11.0 37.0 115-119 32.4388 37.0 37.0 37.0 11.0 37.0 120-124 32.291999999999994 37.0 37.0 37.0 11.0 37.0 125-129 32.4584 37.0 37.0 37.0 11.0 37.0 130-134 32.5145 37.0 37.0 37.0 11.0 37.0 135-139 32.6904 37.0 37.0 37.0 11.0 37.0 140-144 32.825 37.0 37.0 37.0 11.0 37.0 145-149 32.945299999999996 37.0 37.0 37.0 11.0 37.0 150 32.81 37.0 37.0 37.0 11.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.0 20 2.0 21 7.0 22 8.0 23 3.0 24 14.0 25 12.0 26 15.0 27 16.0 28 25.0 29 60.0 30 92.0 31 161.0 32 209.0 33 290.0 34 496.0 35 1482.0 36 1025.0 37 79.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 17.95 22.875 33.1 26.075 2 17.150000000000002 29.875 20.424999999999997 32.550000000000004 3 13.275 23.95 39.45 23.325000000000003 4 19.45 27.1 27.875 25.575 5 19.900000000000002 31.8 25.35 22.95 6 18.224999999999998 31.075000000000003 27.6 23.1 7 18.099999999999998 28.925 26.075 26.900000000000002 8 17.45 33.7 25.724999999999998 23.125 9 18.65 32.324999999999996 26.174999999999997 22.85 10-14 19.005 30.625000000000004 25.365 25.005 15-19 18.44 30.855 26.200000000000003 24.505 20-24 18.4 30.869999999999997 25.36 25.369999999999997 25-29 18.005 30.735 25.465 25.795 30-34 19.56 29.675 27.185 23.580000000000002 35-39 20.96 29.880000000000003 26.529999999999998 22.63 40-44 21.795 28.52 26.36 23.325000000000003 45-49 23.13 28.335 26.83 21.705 50-54 23.025000000000002 28.205000000000002 27.439999999999998 21.33 55-59 24.11 27.91 26.525 21.455 60-64 24.55 27.82 26.99 20.64 65-69 24.740000000000002 27.325 27.125 20.810000000000002 70-74 25.64 25.130000000000003 28.28 20.95 75-79 27.625 24.125 27.455000000000002 20.794999999999998 80-84 27.189999999999998 23.115 28.084999999999997 21.61 85-89 26.465 23.935000000000002 28.24 21.36 90-94 27.589999999999996 25.5 26.995 19.915 95-99 29.92 26.125 25.95 18.005 100-104 33.739999999999995 24.64 25.69 15.93 105-109 39.195 23.919999999999998 22.81 14.075 110-114 46.19 21.060000000000002 20.605 12.145 115-119 51.629999999999995 18.905 17.91 11.555 120-124 56.3 17.28 16.384999999999998 10.035 125-129 60.595 15.885 14.845 8.674999999999999 130-134 65.03999999999999 14.185 13.184999999999999 7.59 135-139 68.915 11.575000000000001 12.345 7.165000000000001 140-144 71.635 11.265 10.565 6.535 145-149 73.33 10.03 10.58 6.0600000000000005 150 74.7 8.924999999999999 10.65 5.7250000000000005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 4.0 2 0.0 3 0.5 4 0.5 5 0.0 6 1.0 7 1.5 8 1.0 9 1.0 10 1.0 11 1.5 12 1.5 13 0.5 14 1.0 15 1.5 16 2.5 17 3.5 18 3.5 19 3.0 20 1.5 21 2.0 22 3.0 23 4.0 24 3.0 25 2.0 26 2.0 27 5.0 28 7.5 29 8.0 30 13.5 31 13.0 32 12.0 33 26.0 34 33.0 35 35.0 36 48.5 37 71.0 38 88.0 39 96.0 40 126.0 41 145.5 42 174.0 43 208.0 44 239.5 45 276.5 46 298.5 47 315.5 48 333.0 49 306.0 50 258.0 51 230.0 52 171.0 53 120.0 54 97.5 55 69.0 56 46.5 57 31.0 58 16.0 59 11.0 60 6.0 61 2.5 62 2.0 63 3.0 64 2.0 65 1.5 66 0.5 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.125 #Duplication Level Percentage of deduplicated Percentage of total 1 89.59881129271918 75.375 2 7.934621099554234 13.350000000000001 3 1.188707280832095 3.0 4 0.44576523031203563 1.5 5 0.17830609212481427 0.75 6 0.1485884101040119 0.75 7 0.05943536404160475 0.35000000000000003 8 0.1485884101040119 1.0 9 0.0 0.0 >10 0.2971768202080238 3.925 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGGAAGGAATGACAAGGTTTCCTTCTCAAGATCATGGAGCACTAACATGT 25 0.625 No Hit AGTTCACAATCCAAAGCCTAATCAGAAAATTGACATCAAATTACCAAATT 21 0.525 No Hit TTGAACGATCGGGGAAATTCGAGCTCCTACTTGTGATCGTCGGACTGTAG 18 0.44999999999999996 No Hit CCTGCTCAAGGCAAGCATCGAGCACCGCATCAGAGATCTGGTCGCATAGT 18 0.44999999999999996 No Hit TCTTTTCTCAGGTAACAATCGAACAACAATCTTCAAAAGAACACGTGATC 15 0.375 No Hit CTGGGTCCTGCTCAAGGCAGGCATCGAGCACTGCATCAGAGATCTGGTCA 14 0.35000000000000003 No Hit TAATGACCGAGACCTTCCTCCAACTCTTCCTCAGGTTGGCTAGCAAACCA 13 0.325 No Hit ATTTTTTTTATTACAATACCAAAGATCACACGTGATCGTCGGACTGTAGA 12 0.3 No Hit CAGCATCAAGACCAACGTCGTCAGAAACAAATCCAATGTGATCGTCGGAC 11 0.27499999999999997 No Hit CCTTACAGGGTTACTCACAGTCTGAAATTGGGATGTGATCGTCGGACTGT 10 0.25 No Hit GCTTTCTTGAGAGAAACTACAACGAGGATCATATTCCAATTCCCAAAGGT 8 0.2 No Hit CCTGCTCAAGGCAGGCATCGAGCACTGCATCAGAGATCTGGTCACATAGT 8 0.2 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.2 No Hit GATCCACAGTCTTTCTCTAAATGATGAAAAGAACCTGACCGACTCATATT 8 0.2 No Hit CATCGACCATATCGGTTTCAGCATCTTCAGAGAAGTTATTCTGTGATCGT 8 0.2 No Hit ATCTTAATGCATGAGATTAGACCATTGAGGATAGCTAACGATTATAGACC 7 0.17500000000000002 No Hit TTGCTTACATCCTCGGAACCACGTCTGTGATCGTCGGACTGTAGAACTCT 7 0.17500000000000002 Illumina DpnII expression Sequencing Primer (100% over 22bp) AGCTTGGGTCCGATCGTTTTGAGCTCAAATTTCTTCACAGACCACGCAAG 6 0.15 No Hit CCTGGCTTTGCTTCTAAGGTCCATACCAGAACCACCGATCCATATGTGAT 6 0.15 No Hit ATGAAACCTCTGGTGCACTTTACGTGGTTTTTCACGTAGGCATAGAAGTT 6 0.15 No Hit AAATGGAAACAAATAATAGCTTCAGAATTGATCAAACCAGATAAATGACA 6 0.15 No Hit CATGGCCTCAACAACAGTCAATCCAAAAGCCTCATACAAAGCAGGCTGCA 6 0.15 No Hit CATCAACCATATCGGTTTCAGCCTCTTCAGAGTAGTTATTCTGTGATCGT 5 0.125 No Hit TTGAGATCCTTGACAGCAACATTCTTAACATTGAAACCAACATTGTGATC 5 0.125 No Hit ACTTGCGAGTCTTGAGTCCACAATCAGGGTTAACCCATAAGATGTGATCG 5 0.125 No Hit TGCCACCGAGAACGGCGAGCAAGTAAGCGGCTACAACCTTCATTGTGATC 5 0.125 No Hit CACGCCGCAAATAGAGGACACAAAGACAATCCTTCCAGCTCCTGAAGCTT 5 0.125 No Hit GCCAAAAGATCGTCAAAAACTAGACCACACATGTGATCGTCGGACTGTAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.025 0.0 0.0 4 0.0 0.0 0.025 0.0 0.0 5 0.0 0.0 0.075 0.0 0.0 6 0.0 0.0 0.075 0.0 0.0 7 0.0 0.0 0.075 0.0 0.0 8 0.0 0.0 0.075 0.0 0.0 9 0.0 0.0 0.1 0.0 0.0 10-11 0.0 0.0 0.225 0.0 0.0 12-13 0.0 0.0 0.2875 0.0 0.0 14-15 0.0 0.0 0.325 0.0 0.0 16-17 0.0 0.0 0.4 0.0 0.0 18-19 0.0 0.0 0.4625 0.0 0.0 20-21 0.0 0.0 0.55 0.0 0.0 22-23 0.0 0.0 0.8375 0.0 0.0 24-25 0.0 0.0 1.2000000000000002 0.0 0.0 26-27 0.0 0.0 1.5875 0.0 0.0 28-29 0.0 0.0 2.4375 0.0 0.0 30-31 0.0 0.0 3.7875 0.0 0.0 32-33 0.0 0.0 5.550000000000001 0.0 0.0 34-35 0.0 0.0 8.149999999999999 0.0 0.0 36-37 0.0 0.0 11.0125 0.0 0.0 38-39 0.0 0.0 13.9375 0.0 0.0 40-41 0.0 0.0 17.1 0.0 0.0 42-43 0.0 0.025 20.25 0.0 0.0 44-45 0.0 0.025 23.1875 0.0 0.0 46-47 0.0 0.025 26.4875 0.0 0.0 48-49 0.0 0.025 30.2125 0.0 0.0 50-51 0.0 0.025 33.675 0.0 0.0 52-53 0.0 0.025 37.1875 0.0 0.0 54-55 0.0 0.025 40.225 0.0 0.0 56-57 0.0 0.025 42.8375 0.0 0.0 58-59 0.0 0.025 45.2625 0.0 0.0 60-61 0.0 0.025 47.7125 0.0 0.0 62-63 0.0 0.025 49.9375 0.0 0.0 64-65 0.0 0.025 52.0875 0.0 0.0 66-67 0.0 0.025 54.3875 0.0 0.0 68-69 0.0 0.025 56.2 0.0 0.0 70-71 0.0 0.025 58.0125 0.0 0.0 72-73 0.0 0.025 59.7625 0.0 0.0 74-75 0.0 0.025 61.7625 0.0 0.0 76-77 0.0 0.025 63.4125 0.0 0.0 78-79 0.0 0.025 64.625 0.0 0.0 80-81 0.0 0.025 65.4875 0.0 0.0 82-83 0.0 0.025 66.5875 0.0 0.0 84-85 0.0 0.025 67.7375 0.0 0.0 86-87 0.0 0.025 68.69999999999999 0.0 0.0 88-89 0.0 0.025 69.4625 0.0 0.0 90-91 0.0 0.025 70.35 0.0 0.0 92-93 0.0 0.025 71.2875 0.0 0.0 94-95 0.0 0.025 72.0875 0.0 0.0 96-97 0.0 0.025 72.775 0.0 0.0 98-99 0.0 0.025 73.5375 0.0 0.0 100-101 0.0 0.025 74.375 0.0 0.0 102-103 0.0 0.025 75.25 0.0 0.0 104-105 0.0 0.025 75.8125 0.0 0.0 106-107 0.0 0.025 76.51249999999999 0.0 0.0 108-109 0.0 0.025 77.05000000000001 0.0 0.0 110-111 0.0 0.025 77.575 0.0 0.0 112-113 0.0 0.025 78.1625 0.0 0.0 114-115 0.0 0.025 78.6875 0.0 0.0 116-117 0.0 0.025 79.0875 0.0 0.0 118-119 0.0 0.025 79.4375 0.0 0.0 120-121 0.0 0.025 79.625 0.0 0.0 122-123 0.0 0.025 79.975 0.0 0.0 124-125 0.0 0.025 80.42500000000001 0.0 0.0 126-127 0.0 0.025 80.9 0.0 0.0 128-129 0.0 0.025 81.2375 0.0 0.0 130-131 0.0 0.025 81.525 0.0 0.0 132-133 0.0 0.025 81.8 0.0 0.0 134-135 0.0 0.025 82.05 0.0 0.0 136-137 0.0 0.025 82.325 0.0 0.0 138 0.0 0.025 82.525 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTTTG 10 0.006973645 144.0 8 ATGACCG 10 0.006973645 144.0 3 TGATCCT 10 0.006973645 144.0 5 TAATGAC 10 0.006973645 144.0 1 CTGGCTT 10 0.006973645 144.0 2 ACCGAGA 10 0.006973645 144.0 6 TGCTTGA 10 0.006973645 144.0 1 GACCGAG 10 0.006973645 144.0 5 CCTTTGA 10 0.006973645 144.0 9 TTGATCC 10 0.006973645 144.0 4 CCGAGAC 10 0.006973645 144.0 7 CGAGACC 10 0.006973645 144.0 8 CTTTGCT 10 0.006973645 144.0 6 TTGCTTC 10 0.006973645 144.0 8 CCTGGCT 10 0.006973645 144.0 1 ATCCTTT 10 0.006973645 144.0 7 >>END_MODULE Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273015 spots for SRR12768913.sra Written 2273015 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra Read 2273011 spots for SRR12768913.sra Written 2273011 spots for SRR12768913.sra SRR ids: ['SRR12768913.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_oudc968j SRR12768913.sra spots: 45460224 blocks: [[1, 2273011], [2273012, 4546022], [4546023, 6819033], [6819034, 9092044], [9092045, 11365055], [11365056, 13638066], [13638067, 15911077], [15911078, 18184088], [18184089, 20457099], [20457100, 22730110], [22730111, 25003121], [25003122, 27276132], [27276133, 29549143], [29549144, 31822154], [31822155, 34095165], [34095166, 36368176], [36368177, 38641187], [38641188, 40914198], [40914199, 43187209], [43187210, 45460224]] SRR12768913 file size 15338883 SRR12768913 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR12768913 SRR12768913_1.fastq SRR12768913_2.fastq Input file: SRR12768913_1.fastq Paired file: SRR12768913_2.fastq trimmed: SRR12768913-trimmed-pair1.fastq, SRR12768913-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Feb 10 21:57:22 2025 >> started Mon Feb 10 21:58:14 2025 >> done (51.867s) 45460224 read pairs processed; of these: 194239 ( 0.43%) short read pairs filtered out after trimming by size control 10208 ( 0.02%) empty read pairs filtered out after trimming by size control 45255777 (99.55%) read pairs available; of these: 39206153 (86.63%) trimmed read pairs available after processing 6049624 (13.37%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 21635 0.05% 19 31233 0.07% 20 33330 0.07% 21 59332 0.13% 22 60596 0.13% 23 76431 0.17% 24 135671 0.30% 25 112062 0.25% 26 154722 0.34% 27 189885 0.42% 28 288023 0.64% 29 262917 0.58% 30 411246 0.91% 31 363106 0.80% 32 353959 0.78% 33 657313 1.45% 34 665705 1.47% 35 579287 1.28% 36 753467 1.66% 37 612361 1.35% 38 621032 1.37% 39 796385 1.76% 40 598812 1.32% 41 719389 1.59% 42 709492 1.57% 43 736354 1.63% 44 853359 1.89% 45 830626 1.84% 46 968029 2.14% 47 724923 1.60% 48 864202 1.91% 49 720459 1.59% 50 1005952 2.22% 51 792420 1.75% 52 739898 1.63% 53 637771 1.41% 54 716389 1.58% 55 611424 1.35% 56 598462 1.32% 57 602895 1.33% 58 494036 1.09% 59 562498 1.24% 60 576870 1.27% 61 473349 1.05% 62 495387 1.09% 63 446138 0.99% 64 472172 1.04% 65 450929 1.00% 66 528719 1.17% 67 381685 0.84% 68 416077 0.92% 69 440806 0.97% 70 629851 1.39% 71 380489 0.84% 72 442866 0.98% 73 418944 0.93% 74 422236 0.93% 75 312822 0.69% 76 307014 0.68% 77 279034 0.62% 78 334331 0.74% 79 252855 0.56% 80 273643 0.60% 81 285260 0.63% 82 215568 0.48% 83 221260 0.49% 84 280962 0.62% 85 213130 0.47% 86 207649 0.46% 87 214145 0.47% 88 203664 0.45% 89 193729 0.43% 90 256075 0.57% 91 181735 0.40% 92 185808 0.41% 93 190021 0.42% 94 189631 0.42% 95 172417 0.38% 96 215149 0.48% 97 146822 0.32% 98 153014 0.34% 99 182764 0.40% 100 138186 0.31% 101 133394 0.29% 102 160272 0.35% 103 140542 0.31% 104 131036 0.29% 105 135858 0.30% 106 123948 0.27% 107 127394 0.28% 108 141232 0.31% 109 129161 0.29% 110 138682 0.31% 111 122550 0.27% 112 108619 0.24% 113 125207 0.28% 114 115228 0.25% 115 111240 0.25% 116 102597 0.23% 117 117155 0.26% 118 111170 0.25% 119 95255 0.21% 120 95460 0.21% 121 97522 0.22% 122 88144 0.19% 123 89008 0.20% 124 99560 0.22% 125 81485 0.18% 126 92398 0.20% 127 83816 0.19% 128 84199 0.19% 129 79915 0.18% 130 79555 0.18% 131 79035 0.17% 132 93470 0.21% 133 69743 0.15% 134 71519 0.16% 135 77635 0.17% 136 71442 0.16% 137 68572 0.15% 138 77406 0.17% 139 66163 0.15% 140 82284 0.18% 141 65617 0.14% 142 63508 0.14% 143 61524 0.14% 144 81784 0.18% 145 66901 0.15% 146 66836 0.15% 147 65672 0.15% 148 64153 0.14% 149 60993 0.13% 150 6049624 13.37% 45255777 reads passed initial QC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=1.75 fanout-score-rank=13 prefix-density=0.92 prefix-fanout=1.0 sequence=ATCTCTGATGCGGTGCTCGATGCTTGCCTTGAGCAGGACCCAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=61.18 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=6.1 sequence=TTTTTCTTCATTTTACGTGTTCTTTTGAAGATTGTTGTTCGATTGTTACCTGAGAAAAGA criterion=sequence-density sequence-density=0.92 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=12 prefix-density=0.96 prefix-fanout=2.2 sequence=GCATCAGAGATCTGGTCGCATAGTTTGT criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=32.20 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=4.8 sequence=CCAGAACCAGTACCACCTCCAACAGCATTGAACACCAAAAACCCTTGAAGGCCAGTGCAGTTATCAGCTAACTTTCGGATACGATCCAAGCAGAGATCAACAATCTCTTTCCCAATGGTATAGTGGCCACGGGCAAAGTTGT SRR12768913 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 10 21:58:43 Started mapping on | Feb 10 21:58:44 Finished on | Feb 10 22:01:24 Mapping speed, Million of reads per hour | 1018.25 Number of input reads | 45255777 Average input read length | 149 UNIQUE READS: Uniquely mapped reads number | 37445057 Uniquely mapped reads % | 82.74% Average mapped length | 132.81 Number of splices: Total | 13470598 Number of splices: Annotated (sjdb) | 13237012 Number of splices: GT/AG | 13207870 Number of splices: GC/AG | 199281 Number of splices: AT/AC | 10712 Number of splices: Non-canonical | 52735 Mismatch rate per base, % | 0.19% Deletion rate per base | 0.02% Deletion average length | 2.02 Insertion rate per base | 0.01% Insertion average length | 1.57 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1666308 % of reads mapped to multiple loci | 3.68% Number of reads mapped to too many loci | 293540 % of reads mapped to too many loci | 0.65% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 12.75% % of reads unmapped: other | 0.18% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6144412 6144412 6144412 N_multimapping 1666308 1666308 1666308 N_noFeature 1763930 1866785 37053225 N_ambiguous 419172 129918 825 UnstrandedReadsAssigned:35261955 PositiveStrandReadsAssigned:35448354 NegativeStrandReadsAssigned:391007 Dataset is classified positive stranded MeadianReadLen=61 20thPercentileLength=42 echo kmer=37 SRR12768913 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR12768913-trimmed-pair1.fastq SRR12768913-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 45,255,777 reads, 34,397,978 reads pseudoaligned [quant] estimated average fragment length: 65.3463 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,241 rounds 52401 SRR12768913.ke.tsv 34699 SRR12768913.se.tsv 87100 total ==> SRR12768913.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1953.65 925.567 20.9492 Potri.005G024800.1.v4.1 1035 970.654 264 12.0267 Potri.004G059700.1.v4.1 961 896.654 39 1.9233 Potri.007G009000.2.v4.1 1416 1351.65 0 0 Potri.003G141000.2.v4.1 2943 2878.65 720.781 11.0719 Potri.016G087400.1.v4.1 270 206.156 381 81.7214 Potri.015G069301.1.v4.1 564 499.66 0 0 Potri.010G195200.1.v4.1 1773 1708.65 107 2.76909 Potri.012G127500.1.v4.1 977 912.654 2612 126.553 ==> SRR12768913.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1911 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 404 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 42 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 13 SRR12768913 completed mapping pipeline successfully