Starting /dee2/code/volunteer_pipeline.sh SRR12768916 current disk space = 3057120509952 free memory = 1339637508 SRR12768916 SRAfilesize 8bd5d9b77939a530f30e490ff5b34f24 SRR12768916.sra SRR12768916.sra file validated SRR12768916 is paired end SRR12768916 is conventional basespace SRR12768916 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR12768916_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61625 37.0 37.0 37.0 37.0 37.0 2 36.642 37.0 37.0 37.0 37.0 37.0 3 36.6065 37.0 37.0 37.0 37.0 37.0 4 36.49 37.0 37.0 37.0 37.0 37.0 5 36.458 37.0 37.0 37.0 37.0 37.0 6 36.5405 37.0 37.0 37.0 37.0 37.0 7 36.4575 37.0 37.0 37.0 37.0 37.0 8 36.433 37.0 37.0 37.0 37.0 37.0 9 36.459 37.0 37.0 37.0 37.0 37.0 10-14 36.4666 37.0 37.0 37.0 37.0 37.0 15-19 36.4317 37.0 37.0 37.0 37.0 37.0 20-24 36.4176 37.0 37.0 37.0 37.0 37.0 25-29 36.299699999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.2916 37.0 37.0 37.0 37.0 37.0 35-39 36.2808 37.0 37.0 37.0 37.0 37.0 40-44 36.2252 37.0 37.0 37.0 37.0 37.0 45-49 36.1794 37.0 37.0 37.0 37.0 37.0 50-54 36.1331 37.0 37.0 37.0 37.0 37.0 55-59 36.06529999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.0695 37.0 37.0 37.0 37.0 37.0 65-69 36.034000000000006 37.0 37.0 37.0 37.0 37.0 70-74 35.7512 37.0 37.0 37.0 37.0 37.0 75-79 35.379799999999996 37.0 37.0 37.0 34.6 37.0 80-84 34.726 37.0 37.0 37.0 25.0 37.0 85-89 33.8532 37.0 37.0 37.0 22.2 37.0 90-94 32.988099999999996 37.0 37.0 37.0 13.8 37.0 95-99 31.7421 37.0 27.4 37.0 11.0 37.0 100-104 30.918799999999997 37.0 25.0 37.0 11.0 37.0 105-109 30.489799999999995 37.0 25.0 37.0 11.0 37.0 110-114 30.6342 37.0 25.0 37.0 11.0 37.0 115-119 31.2077 37.0 25.0 37.0 11.0 37.0 120-124 32.1219 37.0 34.6 37.0 11.0 37.0 125-129 32.7128 37.0 37.0 37.0 11.0 37.0 130-134 33.0642 37.0 37.0 37.0 11.0 37.0 135-139 33.4432 37.0 37.0 37.0 16.6 37.0 140-144 33.84734999999999 37.0 37.0 37.0 25.0 37.0 145-149 34.325399999999995 37.0 37.0 37.0 25.0 37.0 150 34.321 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 0.0 22 0.0 23 6.0 24 5.0 25 13.0 26 26.0 27 36.0 28 40.0 29 46.0 30 75.0 31 88.0 32 151.0 33 420.0 34 1583.0 35 692.0 36 765.0 37 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.4339622641509433 94.31446540880502 2.591194968553459 1.6603773584905661 2 1.375 2.825 5.7 90.10000000000001 3 12.65 70.375 9.75 7.225 4 19.950000000000003 34.875 32.35 12.825000000000001 5 33.074999999999996 24.125 27.800000000000004 15.0 6 28.000000000000004 25.424999999999997 31.474999999999998 15.1 7 23.474999999999998 25.2 34.4 16.925 8 25.924999999999997 23.775 32.525 17.775 9 28.050000000000004 24.349999999999998 31.95 15.65 10-14 26.025 23.97 32.495000000000005 17.51 15-19 25.285000000000004 24.985 31.595000000000002 18.135 20-24 25.195 25.46 31.285 18.060000000000002 25-29 24.415 26.695 31.069999999999997 17.82 30-34 24.45 27.694999999999997 29.915000000000003 17.94 35-39 25.290000000000003 28.065 29.12 17.525 40-44 25.995 29.4 27.1 17.505000000000003 45-49 26.055 29.959999999999997 25.380000000000003 18.605 50-54 26.200000000000003 29.935000000000002 24.435000000000002 19.43 55-59 25.174999999999997 30.325000000000003 23.23 21.27 60-64 23.865 29.695 23.27 23.169999999999998 65-69 22.21 30.490000000000002 23.415 23.885 70-74 21.095 31.155 24.255 23.494999999999997 75-79 20.915 32.1 23.965 23.02 80-84 21.09 30.865 25.72 22.325 85-89 20.105 28.715000000000003 27.68 23.5 90-94 20.51 25.89 30.15 23.45 95-99 22.16 24.32 29.759999999999998 23.76 100-104 25.21 24.395 28.595 21.8 105-109 32.11 23.23 25.555 19.105 110-114 39.37 22.305 22.285 16.04 115-119 46.47 20.005 19.49 14.035 120-124 52.82 17.31 17.244999999999997 12.625 125-129 58.085 15.045 15.58 11.29 130-134 61.19 14.594999999999999 14.555000000000001 9.66 135-139 65.38000000000001 13.594999999999999 12.504999999999999 8.52 140-144 69.57347867393369 11.51557577878894 10.920546027301366 7.990399519975999 145-149 72.35000000000001 10.334999999999999 9.905 7.41 150 74.375 9.825000000000001 9.775 6.0249999999999995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 2.0 9 1.5 10 1.5 11 1.5 12 1.0 13 1.0 14 2.0 15 2.0 16 1.5 17 1.5 18 1.5 19 2.5 20 3.5 21 5.5 22 4.0 23 3.0 24 4.5 25 4.5 26 5.5 27 4.5 28 5.0 29 11.0 30 12.5 31 12.5 32 21.5 33 31.0 34 39.5 35 54.5 36 66.5 37 76.0 38 104.5 39 128.0 40 147.5 41 178.0 42 224.0 43 268.5 44 324.0 45 367.5 46 351.0 47 343.5 48 313.5 49 245.0 50 200.5 51 157.5 52 107.5 53 63.0 54 31.0 55 18.5 56 10.0 57 9.5 58 9.5 59 4.0 60 2.5 61 1.5 62 1.0 63 1.0 64 0.5 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.625 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.25 #Duplication Level Percentage of deduplicated Percentage of total 1 88.46846846846846 73.65 2 8.528528528528529 14.2 3 1.5915915915915915 3.975 4 0.42042042042042044 1.4000000000000001 5 0.2702702702702703 1.125 6 0.3003003003003003 1.5 7 0.09009009009009009 0.525 8 0.06006006006006006 0.4 9 0.03003003003003003 0.22499999999999998 >10 0.24024024024024024 3.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACAAGTAGGAGCTCGAATTTCCCCGATCGTTCAAAGATCGGAAGAGCACA 21 0.525 No Hit ACGTGTTGCTGTTGATATTTAATTTGGTAATTTGATGTCAATTTTCTGAT 21 0.525 No Hit ACATGTTAGTGCTCCATGATCTTGAGAAGGAAACCTTGTCATTCCTTCCA 17 0.42500000000000004 No Hit ACGTGTGATCTTTGGTATTGTAATAAAAAAAATAGATCGGAAGAGCACAC 16 0.4 No Hit ACTATGCGACCAGATCTCTGATGCGGTGCTCGATGCTTGCCTTGAGCAGG 12 0.3 No Hit ACATCCCAATTTCAGACTGTGAGTAACCCTGTAAGGAGATCGGAAGAGCA 12 0.3 No Hit ACGTGTTCTTTTGAAGATTGTTGTTCGATTGTTACCTGAGAAAAGAAGAT 11 0.27499999999999997 No Hit ACCATGTCTGTTGGTACGTGTGATCTTTGGTATTGTAATAAAAAAAATAG 10 0.25 No Hit ACAAACGTGTTGCTGTTGATATTTAATTTGGTAATTTGATGTCAATTTTC 9 0.22499999999999998 No Hit ACAAAACTGTATGGAGTCTATTAAACTTCTATGCCTACGTGAAAAACCAC 8 0.2 No Hit ACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTT 8 0.2 No Hit ACATGATGTGTGCTGCTGATCCTCGCCATGGCAGATATCTCACAGCATCT 7 0.17500000000000002 No Hit ACAGAATTTCGAGCTCTTGCCTCCTCCCGGAGATCGGAAGAGCACACGTC 7 0.17500000000000002 No Hit ACAAACTATGCGACCAGATCTCTGATGCGGTGCTCGATGCTTGCCTTGAG 7 0.17500000000000002 No Hit ACATGATAGAGTGTCTGCTGTTTATGCCCTTCTTTGTTGCTTGGGTATAT 6 0.15 No Hit ACAAAACTGTTGGCGGAGGTGATGATGCTTTCAACACCTTTTTCAGTGAA 6 0.15 No Hit ACAAGACTGTCTATCCGATCGAGAAGGTTACGGTAAATGGGACTCCATAC 6 0.15 No Hit ACATCTTATGGGTTAACCCTGATTGTGGACTCAAGACTCGCAAGTAGATC 6 0.15 No Hit ACATCCACTTTTGAAAGCTTCAGGAGCTGGAAGGATTGTCTTTGTGTCCT 6 0.15 No Hit ACATTTGCTAGAACTCTGTGCTGGAGCCTATCTCTTCTTCTGTGGATGCT 6 0.15 No Hit ACATATGGATCGGTGGATCTGGTATGGATCTTAGAAGCAAAGCCAGGAGA 6 0.15 No Hit ACAAGGATAGCACTCTGATCATGCAACTCCTAAGGGATAACCTCACTCTT 6 0.15 No Hit ACAGAATAACTTCTCTGAAGATGCTGAAACCGATATGGTCGATGAGATCG 6 0.15 No Hit ACAAGATGTTGATGATAATGTGCTTACTGATCTCAAAAGAGATCGGAAGA 6 0.15 No Hit ACAAACTATGTGACCAGATCTCTGATGCAGTGCTCGATGCCTGCCTTGAG 5 0.125 No Hit ACAACGATCAAGAGCGACTGTGCTATTCCTGCAATTCTTGCAAAGCTGGT 5 0.125 No Hit ACATGGGAGACTGTCAAGCTTCTCAAGCCCAATGCCTAAGTCTTTGGGAT 5 0.125 No Hit ACATTGGATTTGTTTCTGACGACGTTGGTCTTGATGCTGAGATCGGAAGA 5 0.125 No Hit ACATCGATCCTTATCATGGTGAGAAGGCTGCTGAGCTCCTTGTTGACTTC 5 0.125 No Hit ACAGAATAACTACTCTGAAGAGGCTGAAACCGATATGGTTGATGAGATCG 5 0.125 No Hit ACATTATGCTTTCGTGCTTTGCGTAGAACTGTGTGCTAATGGGCTGGTCC 5 0.125 No Hit ACCGAAGATGTCTGTCTGATTTTGTGATATACTTAGTATATGTGTATGAT 5 0.125 No Hit ACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.05 0.0 0.0 0.0 0.0 5 0.05 0.0 0.0 0.0 0.0 6 0.05 0.0 0.0 0.0 0.0 7 0.05 0.0 0.0 0.0 0.0 8 0.05 0.0 0.0 0.0 0.0 9 0.05 0.0 0.0 0.0 0.0 10-11 0.2625 0.0 0.0 0.0 0.0 12-13 0.3 0.0 0.0 0.0 0.0 14-15 0.375 0.0 0.0 0.0 0.0 16-17 0.4375 0.0 0.0 0.0 0.0 18-19 0.5375000000000001 0.0 0.0 0.0 0.0 20-21 0.625 0.0 0.0 0.0 0.0 22-23 0.8 0.0 0.0 0.0 0.0 24-25 1.125 0.0 0.0 0.0 0.0 26-27 1.7000000000000002 0.0 0.0 0.0 0.0 28-29 2.425 0.0 0.0 0.0 0.0 30-31 3.8125 0.0 0.0 0.0 0.0 32-33 5.3875 0.0 0.0 0.0 0.0 34-35 8.4 0.0 0.0 0.0 0.0 36-37 11.0375 0.0 0.0 0.0 0.0 38-39 13.75 0.0 0.0 0.0 0.0 40-41 16.775 0.0 0.0 0.0 0.0 42-43 20.2875 0.0 0.0 0.0 0.0 44-45 23.987499999999997 0.0 0.0 0.0 0.0 46-47 27.575 0.0 0.0 0.0 0.0 48-49 31.5 0.0 0.0 0.0 0.0 50-51 35.087500000000006 0.0 0.0 0.0 0.0 52-53 38.8 0.0 0.0 0.0 0.0 54-55 41.85 0.0 0.0 0.0 0.0 56-57 44.125 0.0 0.0 0.0 0.0 58-59 46.025000000000006 0.0 0.0 0.0 0.0 60-61 48.0125 0.0 0.0 0.0 0.0 62-63 50.0 0.0 0.0 0.0 0.0 64-65 51.7 0.0 0.0 0.0 0.0 66-67 53.7625 0.0 0.0 0.0 0.0 68-69 55.837500000000006 0.0 0.0 0.0 0.0 70-71 58.0125 0.0 0.0 0.0 0.0 72-73 59.725 0.0 0.0 0.0 0.0 74-75 61.537499999999994 0.0 0.0 0.0 0.0 76-77 62.9875 0.0 0.0 0.0 0.0 78-79 64.05 0.0 0.0 0.0 0.0 80-81 65.1625 0.0 0.0 0.0 0.0 82-83 66.25 0.0 0.0 0.0 0.0 84-85 67.3875 0.0 0.0 0.0 0.0 86-87 68.275 0.0 0.0 0.0 0.0 88-89 69.0375 0.0 0.0 0.0 0.0 90-91 70.05 0.0 0.0 0.0 0.0 92-93 71.0875 0.0 0.0 0.0 0.0 94-95 71.875 0.0 0.0 0.0 0.0 96-97 72.55000000000001 0.0 0.0 0.0 0.0 98-99 73.25 0.0 0.0 0.0 0.0 100-101 74.0125 0.0 0.0 0.0 0.0 102-103 74.3375 0.0 0.0 0.0 0.0 104-105 75.05 0.0 0.0 0.0 0.0 106-107 75.575 0.0 0.0 0.0 0.0 108-109 76.0125 0.0 0.0 0.0 0.0 110-111 76.5375 0.0 0.0 0.0 0.0 112-113 77.15 0.0 0.0 0.0 0.0 114-115 77.6125 0.0 0.0 0.0 0.0 116-117 78.15 0.0 0.0 0.0 0.0 118-119 78.65 0.0 0.0 0.0 0.0 120-121 79.0625 0.0 0.0 0.0 0.0 122-123 79.61250000000001 0.0 0.0 0.0 0.0 124-125 79.9625 0.0 0.0 0.0 0.0 126-127 80.32499999999999 0.0 0.0 0.0 0.0 128-129 80.7375 0.0 0.0 0.0 0.0 130-131 81.19999999999999 0.0 0.0 0.0 0.0 132-133 81.73750000000001 0.0 0.0 0.0 0.0 134-135 82.11250000000001 0.0 0.0 0.0 0.0 136-137 82.3625 0.0 0.0 0.0 0.0 138 82.45 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGATG 10 0.006973645 144.0 2 ACATGAT 10 0.006973645 144.0 1 ATTGGTA 10 0.006973645 144.0 8 TGATTGG 10 0.006973645 144.0 6 GATTGGT 10 0.006973645 144.0 7 ACATTGT 10 0.006973645 144.0 1 ACATCAA 15 1.1730364E-4 144.0 1 ACAATGT 10 0.006973645 144.0 1 ACAATCT 10 0.006973645 144.0 1 GGGGGGG 6155 0.0 5.380991 140-144 >>END_MODULE SRR12768916 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR12768916_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0555 37.0 37.0 37.0 37.0 37.0 2 36.149 37.0 37.0 37.0 37.0 37.0 3 36.3495 37.0 37.0 37.0 37.0 37.0 4 36.479 37.0 37.0 37.0 37.0 37.0 5 36.52 37.0 37.0 37.0 37.0 37.0 6 36.711 37.0 37.0 37.0 37.0 37.0 7 36.6785 37.0 37.0 37.0 37.0 37.0 8 36.64 37.0 37.0 37.0 37.0 37.0 9 36.5655 37.0 37.0 37.0 37.0 37.0 10-14 36.577549999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.483799999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.4645 37.0 37.0 37.0 37.0 37.0 25-29 36.4188 37.0 37.0 37.0 37.0 37.0 30-34 36.414 37.0 37.0 37.0 37.0 37.0 35-39 36.369600000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.3765 37.0 37.0 37.0 37.0 37.0 45-49 36.3552 37.0 37.0 37.0 37.0 37.0 50-54 36.3281 37.0 37.0 37.0 37.0 37.0 55-59 36.2959 37.0 37.0 37.0 37.0 37.0 60-64 36.2484 37.0 37.0 37.0 37.0 37.0 65-69 36.1545 37.0 37.0 37.0 37.0 37.0 70-74 36.060500000000005 37.0 37.0 37.0 37.0 37.0 75-79 36.0087 37.0 37.0 37.0 37.0 37.0 80-84 35.892849999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.5 37.0 37.0 37.0 37.0 37.0 90-94 34.964800000000004 37.0 37.0 37.0 27.4 37.0 95-99 34.23935 37.0 37.0 37.0 25.0 37.0 100-104 33.380300000000005 37.0 37.0 37.0 11.0 37.0 105-109 32.90689999999999 37.0 37.0 37.0 11.0 37.0 110-114 32.363749999999996 37.0 37.0 37.0 11.0 37.0 115-119 32.3327 37.0 37.0 37.0 11.0 37.0 120-124 32.282799999999995 37.0 37.0 37.0 11.0 37.0 125-129 31.948300000000007 37.0 34.6 37.0 11.0 37.0 130-134 31.69545 37.0 29.8 37.0 11.0 37.0 135-139 31.658350000000002 37.0 29.8 37.0 11.0 37.0 140-144 31.843200000000003 37.0 34.6 37.0 11.0 37.0 145-149 31.79805 37.0 32.2 37.0 11.0 37.0 150 31.82725 37.0 37.0 37.0 11.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 11.0 23 11.0 24 7.0 25 15.0 26 15.0 27 14.0 28 17.0 29 54.0 30 132.0 31 209.0 32 250.0 33 289.0 34 420.0 35 1036.0 36 1431.0 37 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.675 24.5 34.875 24.95 2 17.549999999999997 29.349999999999998 21.7 31.4 3 12.725 24.125 39.0 24.15 4 17.95 27.0 29.575000000000003 25.474999999999998 5 20.025000000000002 30.775000000000002 25.55 23.65 6 16.825000000000003 30.599999999999998 28.749999999999996 23.825 7 17.05 28.725 26.375 27.85 8 16.425 34.300000000000004 26.125 23.150000000000002 9 17.599999999999998 31.6 26.924999999999997 23.875 10-14 17.92589629481474 30.72153607680384 25.84629231461573 25.506275313765688 15-19 17.745 30.835 26.534999999999997 24.884999999999998 20-24 18.11 31.685000000000002 25.345000000000002 24.86 25-29 18.555 31.4 25.490000000000002 24.555 30-34 19.645000000000003 29.935000000000002 27.060000000000002 23.36 35-39 21.39 29.785 26.5 22.325 40-44 22.03 28.189999999999998 26.950000000000003 22.830000000000002 45-49 23.14 27.865000000000002 27.595 21.4 50-54 23.02 28.525 27.355 21.099999999999998 55-59 23.66 28.144999999999996 26.640000000000004 21.555 60-64 23.380000000000003 28.18 27.375 21.065 65-69 24.21 27.605 27.224999999999998 20.96 70-74 25.679999999999996 25.045 28.26 21.015 75-79 27.029999999999998 23.674999999999997 27.735 21.560000000000002 80-84 26.651332566628334 23.926196309815488 28.286414320716034 21.13605680284014 85-89 26.169999999999998 24.43 27.6 21.8 90-94 26.5 26.205000000000002 27.224999999999998 20.07 95-99 27.461373068653433 27.481374068703435 26.896344817240863 18.160908045402273 100-104 29.74 27.98 26.575 15.705 105-109 35.46 27.255000000000003 23.77 13.514999999999999 110-114 42.337116855842794 23.781189059452974 21.52607630381519 12.355617780889045 115-119 47.260000000000005 21.005 19.88 11.855 120-124 50.955 20.94 17.775 10.33 125-129 55.815000000000005 19.295 15.634999999999998 9.254999999999999 130-134 59.60298014900744 17.630881544077205 14.740737036851842 8.025401270063504 135-139 63.373168658432924 14.830741537076852 14.480724036201812 7.315365768288415 140-144 66.405 14.249999999999998 12.889999999999999 6.455 145-149 69.15845792289615 12.850642532126608 12.025601280064004 5.965298264913246 150 72.06801700425106 11.302825706426606 10.552638159539885 6.0765191297824455 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 6.5 2 4.5 3 5.0 4 3.0 5 1.0 6 0.5 7 1.0 8 1.0 9 0.0 10 1.5 11 2.0 12 0.5 13 2.0 14 3.0 15 2.5 16 2.0 17 0.5 18 0.0 19 3.0 20 3.5 21 2.0 22 2.5 23 1.5 24 2.5 25 5.5 26 9.0 27 10.5 28 6.5 29 5.0 30 10.0 31 15.5 32 15.0 33 20.0 34 36.0 35 45.5 36 62.0 37 89.5 38 94.0 39 94.0 40 103.5 41 145.0 42 188.0 43 215.5 44 263.5 45 309.5 46 327.5 47 316.5 48 315.0 49 293.5 50 233.5 51 185.5 52 156.5 53 123.0 54 89.0 55 62.5 56 38.5 57 22.0 58 14.0 59 7.0 60 3.0 61 1.0 62 1.0 63 2.5 64 3.0 65 2.5 66 1.0 67 1.0 68 1.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.005 85-89 0.0 90-94 0.0 95-99 0.005 100-104 0.0 105-109 0.0 110-114 0.005 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.005 135-139 0.005 140-144 0.0 145-149 0.005 150 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.975 #Duplication Level Percentage of deduplicated Percentage of total 1 88.76167520337451 73.65 2 8.315757758360952 13.8 3 1.4160891834890026 3.5249999999999995 4 0.5122024706236818 1.7000000000000002 5 0.24103645676408555 1.0 6 0.24103645676408555 1.2 7 0.1807773425730642 1.05 8 0.06025911419102139 0.4 9 0.0 0.0 >10 0.27116601385959627 3.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGTTCACAATCCAAAGCCTAATCAGAAAATTGACATCAAATTACCAAATT 26 0.65 No Hit TTGAACGATCGGGGAAATTCGAGCTCCTACTTGTGATCGTCGGACTGTAG 22 0.5499999999999999 No Hit CCTGCTCAAGGCAAGCATCGAGCACCGCATCAGAGATCTGGTCGCATAGT 20 0.5 No Hit TGGAAGGAATGACAAGGTTTCCTTCTCAAGATCATGGAGCACTAACATGT 16 0.4 No Hit ATTTTTTTTATTACAATACCAAAGATCACACGTGATCGTCGGACTGTAGA 16 0.4 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.35000000000000003 No Hit CCTTACAGGGTTACTCACAGTCTGAAATTGGGATGTGATCGTCGGACTGT 12 0.3 No Hit TCTTTTCTCAGGTAACAATCGAACAACAATCTTCAAAAGAACACGTGATC 11 0.27499999999999997 No Hit ATTTTTTTTATTACAATACCAAAGATCACACGTACCAACAGACATGGTGA 10 0.25 No Hit AGCTGCTCCCGCACCTATTAATTTAGCACCTTCTAACATTCTCTTTTTTC 8 0.2 No Hit TCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAATG 8 0.2 No Hit ATCAAGCCAATCCCAAAGACTTAGGCATTGGGCTTGAGAAGCTTGACAGT 7 0.17500000000000002 No Hit ATGAAACCTCTGGTGCACTTTACGTGGTTTTTCACGTAGGCATAGAAGTT 7 0.17500000000000002 No Hit GCTCATTTTGCCACGAAACATTGCAGATGCTGTGAGATATCTGCCATGGC 7 0.17500000000000002 No Hit CATGGCCTCAACAACAGTCAATCCAAAAGCCTCATACAAAGCAGGCTGCA 7 0.17500000000000002 No Hit TCTTTCCAAAGGCAAAATCATAGCATCCACAGAAGAAGAGATAGGCTCCA 7 0.17500000000000002 No Hit CATCGACCATATCGGTTTCAGCATCTTCAGAGAAGTTATTCTGTGATCGT 7 0.17500000000000002 No Hit CTGGGTCCTGCTCAAGGCAGGCATCGAGCACTGCATCAGAGATCTGGTCA 6 0.15 No Hit CCGGGAGGAGGCAAGAGCTCGAAATTCTGTGATCGTCGGACTGTAGAACT 6 0.15 No Hit CCTGGCTTTGCTTCTAAGATCCATACCAGATCCACCGATCCATATGTGAT 6 0.15 No Hit CCTGGACATCCGAAGTCCAAAGAGTGAGGTTATCCCTTAGGAGTTGCATG 6 0.15 No Hit GATATACCCAAGCAACAAAGAAGGGCATAAACAGCAGACACTCTATCATG 6 0.15 No Hit ACTTGCGAGTCTTGAGTCCACAATCAGGGTTAACCCATAAGATGTGATCG 6 0.15 No Hit TAATGACCGAGACCTTCCTCCAACTCTTCCTCAGGTTGGCTAGCAAACCA 6 0.15 No Hit CACGCCGCAAATAGAGGACACAAAGACAATCCTTCCAGCTCCTGAAGCTT 6 0.15 No Hit CATCAACCATATCGGTTTCAGCCTCTTCAGAGTAGTTATTCTGTGATCGT 5 0.125 No Hit TACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTGA 5 0.125 No Hit ATCTTAATGCATGAGATTAGACCATTGAGGATAGCTAACGATTATAGACC 5 0.125 No Hit CCCAGTGAGCGGGATCGACCTTGCACTTCTCAAAGAAGTCAACAAGGAGC 5 0.125 No Hit CAAAGTCACTAACAGTCCATTCAGCAACTTGATTAGGGGCCTTGAATATC 5 0.125 No Hit GATCCACAGTCTTTCTCTAAATGATGAAAAGAACCTGACCGACTCATATT 5 0.125 No Hit CAGCATCAAGACCAACGTCGTCAGAAACAAATCCAATGTGATCGTCGGAC 5 0.125 No Hit CTTCTTCCAGGAAAACAGTTCGGCCACCATGATTTAGAGATGTGATCGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.025 0.0 0.0 4 0.0 0.0 0.05 0.0 0.0 5 0.0 0.0 0.05 0.0 0.0 6 0.0 0.0 0.075 0.0 0.0 7 0.0 0.0 0.075 0.0 0.0 8 0.0 0.0 0.075 0.0 0.0 9 0.0 0.0 0.075 0.0 0.0 10-11 0.0 0.0 0.275 0.0 0.0 12-13 0.0 0.0 0.3 0.0 0.0 14-15 0.0 0.0 0.375 0.0 0.0 16-17 0.0 0.0 0.4375 0.0 0.0 18-19 0.0 0.0 0.525 0.0 0.0 20-21 0.0 0.0 0.6 0.0 0.0 22-23 0.0 0.0 0.775 0.0 0.0 24-25 0.0 0.0 1.0499999999999998 0.0 0.0 26-27 0.0 0.0 1.625 0.0 0.0 28-29 0.0 0.0 2.3375 0.0 0.0 30-31 0.0 0.0 3.6500000000000004 0.0 0.0 32-33 0.0 0.0 5.175 0.0 0.0 34-35 0.0 0.0 8.125 0.0 0.0 36-37 0.0 0.0 10.7375 0.0 0.0 38-39 0.0 0.0 13.2875 0.0 0.0 40-41 0.0 0.0 16.175 0.0 0.0 42-43 0.0 0.0 19.6125 0.0 0.0 44-45 0.0 0.0 23.1625 0.0 0.0 46-47 0.0 0.0 26.637500000000003 0.0 0.0 48-49 0.0 0.0 30.5375 0.0 0.0 50-51 0.0 0.0 34.0875 0.0 0.0 52-53 0.0 0.0 37.8125 0.0 0.0 54-55 0.0 0.0 40.7375 0.0 0.0 56-57 0.0 0.0 42.95 0.0 0.0 58-59 0.0 0.0 44.875 0.0 0.0 60-61 0.0 0.0 46.8625 0.0 0.0 62-63 0.0 0.0 48.825 0.0 0.0 64-65 0.0 0.0 50.4625 0.0 0.0 66-67 0.0 0.0 52.5375 0.0 0.0 68-69 0.0 0.0 54.575 0.0 0.0 70-71 0.0 0.0 56.7625 0.0 0.0 72-73 0.0 0.0 58.4875 0.0 0.0 74-75 0.0 0.0 60.3 0.0 0.0 76-77 0.0 0.0 61.725 0.0 0.0 78-79 0.0 0.0 62.7625 0.0 0.0 80-81 0.0 0.0 63.875 0.0 0.0 82-83 0.0 0.0 64.95 0.0 0.0 84-85 0.0 0.0 66.05 0.0 0.0 86-87 0.0 0.0 66.95 0.0 0.0 88-89 0.0 0.0 67.76249999999999 0.0 0.0 90-91 0.0 0.0 68.80000000000001 0.0 0.0 92-93 0.0 0.0 69.86250000000001 0.0 0.0 94-95 0.0 0.0 70.7 0.0 0.0 96-97 0.0 0.0 71.42500000000001 0.0 0.0 98-99 0.0 0.0 72.125 0.0 0.0 100-101 0.0 0.0 72.9125 0.0 0.0 102-103 0.0 0.0 73.2375 0.0 0.0 104-105 0.0 0.0 73.95 0.0 0.0 106-107 0.0 0.0 74.475 0.0 0.0 108-109 0.0 0.0 74.92500000000001 0.0 0.0 110-111 0.0 0.0 75.4625 0.0 0.0 112-113 0.0 0.0 76.0625 0.0 0.0 114-115 0.0 0.0 76.5125 0.0 0.0 116-117 0.0 0.0 77.05000000000001 0.0 0.0 118-119 0.0 0.0 77.5375 0.0 0.0 120-121 0.0 0.0 77.98750000000001 0.0 0.0 122-123 0.0 0.0 78.57499999999999 0.0 0.0 124-125 0.0 0.0 78.98750000000001 0.0 0.0 126-127 0.0 0.0 79.325 0.0 0.0 128-129 0.0 0.0 79.75 0.0 0.0 130-131 0.0 0.0 80.2125 0.0 0.0 132-133 0.0 0.0 80.75 0.0 0.0 134-135 0.0 0.0 81.13749999999999 0.0 0.0 136-137 0.0 0.0 81.3875 0.0 0.0 138 0.0 0.0 81.5 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGCCA 10 0.006973645 144.0 9 AAAAAAA 175 0.009680958 7.405714 90-94 GGGGGGG 6165 0.0 5.021898 140-144 >>END_MODULE Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777376 spots for SRR12768916.sra Written 2777376 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra Read 2777373 spots for SRR12768916.sra Written 2777373 spots for SRR12768916.sra SRR ids: ['SRR12768916.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_18dqdhhv SRR12768916.sra spots: 55547463 blocks: [[1, 2777373], [2777374, 5554746], [5554747, 8332119], [8332120, 11109492], [11109493, 13886865], [13886866, 16664238], [16664239, 19441611], [19441612, 22218984], [22218985, 24996357], [24996358, 27773730], [27773731, 30551103], [30551104, 33328476], [33328477, 36105849], [36105850, 38883222], [38883223, 41660595], [41660596, 44437968], [44437969, 47215341], [47215342, 49992714], [49992715, 52770087], [52770088, 55547463]] SRR12768916 file size 18747266 SRR12768916 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR12768916 SRR12768916_1.fastq SRR12768916_2.fastq Input file: SRR12768916_1.fastq Paired file: SRR12768916_2.fastq trimmed: SRR12768916-trimmed-pair1.fastq, SRR12768916-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Mon Feb 10 22:14:32 2025 >> started Mon Feb 10 22:16:05 2025 >> done (92.501s) 55547463 read pairs processed; of these: 214968 ( 0.39%) short read pairs filtered out after trimming by size control 10230 ( 0.02%) empty read pairs filtered out after trimming by size control 55322265 (99.59%) read pairs available; of these: 47295676 (85.49%) trimmed read pairs available after processing 8026589 (14.51%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 24450 0.04% 19 35976 0.07% 20 37946 0.07% 21 67446 0.12% 22 69259 0.13% 23 87272 0.16% 24 152235 0.28% 25 126764 0.23% 26 175170 0.32% 27 217106 0.39% 28 328554 0.59% 29 300837 0.54% 30 470572 0.85% 31 418343 0.76% 32 408487 0.74% 33 770275 1.39% 34 767759 1.39% 35 670916 1.21% 36 880359 1.59% 37 711377 1.29% 38 720821 1.30% 39 923808 1.67% 40 698408 1.26% 41 844731 1.53% 42 826196 1.49% 43 861928 1.56% 44 1001627 1.81% 45 972514 1.76% 46 1146711 2.07% 47 853130 1.54% 48 1029334 1.86% 49 854030 1.54% 50 1196671 2.16% 51 941158 1.70% 52 884248 1.60% 53 771351 1.39% 54 853715 1.54% 55 731108 1.32% 56 711985 1.29% 57 721398 1.30% 58 592369 1.07% 59 667266 1.21% 60 693175 1.25% 61 571421 1.03% 62 593737 1.07% 63 536188 0.97% 64 572937 1.04% 65 544853 0.98% 66 635219 1.15% 67 465971 0.84% 68 512339 0.93% 69 540243 0.98% 70 794445 1.44% 71 470843 0.85% 72 539015 0.97% 73 515847 0.93% 74 524508 0.95% 75 384248 0.69% 76 378255 0.68% 77 342434 0.62% 78 415065 0.75% 79 312734 0.57% 80 340815 0.62% 81 352544 0.64% 82 269704 0.49% 83 272955 0.49% 84 345550 0.62% 85 263717 0.48% 86 258747 0.47% 87 266716 0.48% 88 254685 0.46% 89 242942 0.44% 90 317899 0.57% 91 227729 0.41% 92 231891 0.42% 93 238307 0.43% 94 236591 0.43% 95 217016 0.39% 96 272039 0.49% 97 183948 0.33% 98 192452 0.35% 99 230176 0.42% 100 172716 0.31% 101 167545 0.30% 102 202225 0.37% 103 178814 0.32% 104 167014 0.30% 105 172300 0.31% 106 158490 0.29% 107 161596 0.29% 108 178755 0.32% 109 166149 0.30% 110 178479 0.32% 111 156219 0.28% 112 139282 0.25% 113 160443 0.29% 114 146238 0.26% 115 142848 0.26% 116 131845 0.24% 117 149283 0.27% 118 142942 0.26% 119 122159 0.22% 120 122896 0.22% 121 125653 0.23% 122 114206 0.21% 123 115921 0.21% 124 128093 0.23% 125 104330 0.19% 126 120066 0.22% 127 108991 0.20% 128 109134 0.20% 129 102981 0.19% 130 102882 0.19% 131 101890 0.18% 132 120576 0.22% 133 89435 0.16% 134 92875 0.17% 135 101476 0.18% 136 92666 0.17% 137 87900 0.16% 138 100836 0.18% 139 85167 0.15% 140 107238 0.19% 141 85776 0.16% 142 82303 0.15% 143 80774 0.15% 144 107199 0.19% 145 86610 0.16% 146 86303 0.16% 147 86032 0.16% 148 83152 0.15% 149 81463 0.15% 150 8026589 14.51% 55322265 reads passed initial QC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=1.74 fanout-score-rank=12 prefix-density=0.91 prefix-fanout=1.0 sequence=ATCTCTGATGCGGTGCTCGATGCTTGCCTTGAGCAGGACCCAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=70.15 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=6.8 sequence=TTTTTCTTCATTTTACGTGTTCTTTTGAAGATTGTTGTTCGATTGTTACCTGAGAAAAGA criterion=sequence-density sequence-density=0.91 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=14 prefix-density=0.94 prefix-fanout=2.2 sequence=GCATCAGAGATCTGGTCGCATAGTTTGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=17.04 fanout-score-rank=1 prefix-density=0.03 prefix-fanout=6.4 sequence=TTTTTTTTTCTTTTCTCAGGTAACAATCGAACAACAATCTTCAAAAGAACACGTAAAATGAAGAAAA SRR12768916 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 10 22:16:38 Started mapping on | Feb 10 22:16:39 Finished on | Feb 10 22:20:26 Mapping speed, Million of reads per hour | 877.36 Number of input reads | 55322265 Average input read length | 153 UNIQUE READS: Uniquely mapped reads number | 45417656 Uniquely mapped reads % | 82.10% Average mapped length | 135.28 Number of splices: Total | 16412000 Number of splices: Annotated (sjdb) | 16119681 Number of splices: GT/AG | 16090168 Number of splices: GC/AG | 243801 Number of splices: AT/AC | 12914 Number of splices: Non-canonical | 65117 Mismatch rate per base, % | 0.20% Deletion rate per base | 0.02% Deletion average length | 2.04 Insertion rate per base | 0.01% Insertion average length | 1.58 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1971942 % of reads mapped to multiple loci | 3.56% Number of reads mapped to too many loci | 348746 % of reads mapped to too many loci | 0.63% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 13.53% % of reads unmapped: other | 0.18% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7932667 7932667 7932667 N_multimapping 1971942 1971942 1971942 N_noFeature 2183353 2308512 44933001 N_ambiguous 518162 158359 999 UnstrandedReadsAssigned:42716141 PositiveStrandReadsAssigned:42950785 NegativeStrandReadsAssigned:483656 Dataset is classified positive stranded MeadianReadLen=62 20thPercentileLength=43 echo kmer=39 SRR12768916 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR12768916-trimmed-pair1.fastq SRR12768916-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 55,322,265 reads, 41,758,176 reads pseudoaligned [quant] estimated average fragment length: 66.1557 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,214 rounds 52401 SRR12768916.ke.tsv 34699 SRR12768916.se.tsv 87100 total ==> SRR12768916.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1952.84 1044 19.4603 Potri.005G024800.1.v4.1 1035 969.844 360 13.5119 Potri.004G059700.1.v4.1 961 895.844 44 1.78787 Potri.007G009000.2.v4.1 1416 1350.84 0 0 Potri.003G141000.2.v4.1 2943 2877.84 853.041 10.7899 Potri.016G087400.1.v4.1 270 205.371 512 90.7502 Potri.015G069301.1.v4.1 564 498.857 0 0 Potri.010G195200.1.v4.1 1773 1707.84 165.492 3.52732 Potri.012G127500.1.v4.1 977 911.844 3240 129.342 ==> SRR12768916.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2159 Potri.001G233950.v4.1 3 Potri.001G122700.v4.1 513 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 56 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 9 SRR12768916 completed mapping pipeline successfully