Starting /dee2/code/volunteer_pipeline.sh SRR13695406 current disk space = 3051218055168 free memory = 1506960992 SRR13695406 SRAfilesize 1c5d3461fca01042d26b856925662511 SRR13695406.sra SRR13695406.sra file validated SRR13695406 is paired end SRR13695406 is conventional basespace SRR13695406 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695406_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5355 37.0 37.0 37.0 37.0 37.0 2 36.197 37.0 37.0 37.0 37.0 37.0 3 36.6115 37.0 37.0 37.0 37.0 37.0 4 36.553 37.0 37.0 37.0 37.0 37.0 5 36.528 37.0 37.0 37.0 37.0 37.0 6 36.612 37.0 37.0 37.0 37.0 37.0 7 36.4945 37.0 37.0 37.0 37.0 37.0 8 36.5235 37.0 37.0 37.0 37.0 37.0 9 36.6045 37.0 37.0 37.0 37.0 37.0 10-14 36.54129999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.550799999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.525600000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.4517 37.0 37.0 37.0 37.0 37.0 30-34 36.40859999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.4039 37.0 37.0 37.0 37.0 37.0 40-44 36.367999999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.4108 37.0 37.0 37.0 37.0 37.0 50-54 36.3601 37.0 37.0 37.0 37.0 37.0 55-59 36.366099999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.330499999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.296400000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.2337 37.0 37.0 37.0 37.0 37.0 75-79 36.2876 37.0 37.0 37.0 37.0 37.0 80-84 36.1694 37.0 37.0 37.0 37.0 37.0 85-89 36.174 37.0 37.0 37.0 37.0 37.0 90-94 36.1033 37.0 37.0 37.0 37.0 37.0 95-99 36.0575 37.0 37.0 37.0 37.0 37.0 100-104 36.0523 37.0 37.0 37.0 37.0 37.0 105-109 36.11130000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.040000000000006 37.0 37.0 37.0 37.0 37.0 115-119 36.04129999999999 37.0 37.0 37.0 37.0 37.0 120-124 35.9568 37.0 37.0 37.0 37.0 37.0 125-129 35.8977 37.0 37.0 37.0 37.0 37.0 130-134 35.865500000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.896 37.0 37.0 37.0 37.0 37.0 140-144 35.7727 37.0 37.0 37.0 37.0 37.0 145-149 35.598699999999994 37.0 37.0 37.0 37.0 37.0 150-151 35.55375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 7.0 26 3.0 27 18.0 28 18.0 29 16.0 30 32.0 31 29.0 32 39.0 33 91.0 34 125.0 35 324.0 36 2938.0 37 359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.574999999999996 10.95 9.55 47.925000000000004 2 17.780010045203415 14.490205926670017 38.67403314917127 29.0557508789553 3 18.15 17.625 24.625 39.6 4 22.05 25.6 22.275 30.075000000000003 5 23.150000000000002 30.099999999999998 25.424999999999997 21.325 6 18.7 36.7 25.074999999999996 19.525000000000002 7 16.925 27.250000000000004 39.025 16.8 8 17.825 25.05 34.875 22.25 9 17.625 22.325 35.5 24.55 10-14 18.61 29.92 28.634999999999998 22.835 15-19 19.900000000000002 28.04 27.625 24.435000000000002 20-24 19.375 28.465 28.535 23.625 25-29 19.685 29.145 27.73 23.44 30-34 19.505 27.544999999999998 28.305000000000003 24.645 35-39 20.39 28.315 27.065 24.23 40-44 19.21 28.77 27.99 24.03 45-49 20.25 28.665000000000003 27.62 23.465 50-54 20.41 28.13 27.98 23.48 55-59 20.31 28.144999999999996 27.650000000000002 23.895 60-64 20.06 27.665 28.645 23.630000000000003 65-69 19.744999999999997 28.310000000000002 28.285 23.66 70-74 20.09 28.549999999999997 27.68 23.68 75-79 20.485 28.51 27.584999999999997 23.419999999999998 80-84 20.01 27.894999999999996 28.64 23.455000000000002 85-89 20.41 27.744999999999997 27.985 23.86 90-94 20.445 29.425 27.500000000000004 22.63 95-99 20.74 28.12 27.925 23.215 100-104 21.23 28.065 27.425 23.28 105-109 20.105 29.580000000000002 27.24 23.075000000000003 110-114 20.49 28.7 27.575 23.235 115-119 20.61 28.43 27.6 23.36 120-124 20.185 28.765 28.09 22.96 125-129 20.44 28.475 27.515 23.57 130-134 20.7 28.475 27.83 22.994999999999997 135-139 20.849999999999998 28.17 28.17 22.81 140-144 20.75 27.884999999999998 27.655 23.71 145-149 21.26 27.91 27.355 23.474999999999998 150-151 21.6625 27.425 27.3375 23.575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 2.0 22 2.5 23 0.5 24 1.5 25 3.5 26 3.0 27 9.0 28 14.0 29 13.0 30 20.5 31 29.5 32 31.5 33 42.5 34 61.0 35 83.5 36 93.5 37 105.0 38 129.0 39 158.5 40 204.5 41 248.5 42 251.5 43 223.0 44 234.5 45 260.0 46 258.5 47 242.0 48 217.5 49 217.5 50 184.0 51 145.5 52 133.5 53 85.0 54 56.0 55 55.0 56 44.5 57 29.0 58 27.0 59 30.5 60 17.0 61 7.0 62 7.5 63 4.5 64 2.0 65 1.5 66 2.5 67 2.0 68 1.0 69 0.0 70 0.0 71 1.0 72 1.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.824999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 65.77795463589335 41.325 2 20.01591723040191 25.15 3 7.759649820931158 14.625 4 3.8201352964584165 9.6 5 1.5121368881814563 4.75 6 0.6764822920811778 2.55 7 0.3581376840429765 1.575 8 0.03979307600477517 0.2 9 0.03979307600477517 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTCATCCCAGTTGCTTTCTCTATCAACTTGATCAAATTCAATGCCTTTG 9 0.22499999999999998 No Hit TAACTTTTTGCCATCTCCCCATTATACAATGGATTCGACAAAGTCCCGTG 8 0.2 No Hit CTGGCCTTCGTATGAATTTTTCTCCAAACCGTCCGATTAAAGGAGTATCC 7 0.17500000000000002 No Hit CCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCA 7 0.17500000000000002 No Hit CATAAAGTGGTCATAAAACAGTGATTAATCCATCGCCGTCTCTCTCCTTT 7 0.17500000000000002 No Hit GCCATCTCTACAATCATTGCTTTAGTGATCATTTCACCTGAGAAGCAAAC 7 0.17500000000000002 No Hit CCGGAAAGCCGCCTTCAGCCTTCCTTCTCTTCCCTATCGCCCAAGACAGT 7 0.17500000000000002 No Hit CACAGGTTGCAATACCACACATGTTCTTCCCCATCTCCATCTTAAAGTAA 7 0.17500000000000002 No Hit CTCCTTTTCTCTTTCCTTTTCTCTTTCCTTTTCTCTTTCCTTTTCTCTAA 7 0.17500000000000002 No Hit ATCAGCAGCCACCACAATAATAGCAATGTCAGTCACCCTCGCTCCACGGG 7 0.17500000000000002 No Hit AGCAGAACAATCAGTACTATGGCATTGTTGGTATGATCAGCATGAGAGCT 7 0.17500000000000002 No Hit CCAAAACCTTTAAATCTGCTAATTGCTTCACGATCGAAAAACTTTGCAGT 6 0.15 No Hit TTCCCATGTTCGGCCTGGATCATTATAGCGAGCGAGCAAACAACCCATCT 6 0.15 No Hit TGAGGCTTGACATCTCTATGCATTATTCCTTGCGAGTGACAGTAATCTAG 6 0.15 No Hit CACGCAATTCCTTTAATTTCTCATCATCCTTGTAAAATGTAGGATTCCTA 6 0.15 No Hit GAAAGACTCCGAAGCACCCGGTGTTTTGCTTCTCCGCAGATAACCCACTC 6 0.15 No Hit GCTCCTAGCACTTTCCTAGCAGTCTCCACTGAAGAGGTAAGAGATGATGA 6 0.15 No Hit GCCTCTCTTCTTCAAAAATATGCGGCATAGAACCCAATTTTCCATTGGCA 6 0.15 No Hit ACTTGACAAGAATATTGATGGCTTCCTCTGTGCTGCCACCATGCCCTTGT 6 0.15 No Hit GCAAGATACAACATGGAATCACCCAATAAAGCTCATGTTTAATAAATAAA 6 0.15 No Hit TTCTCTTCATTTTTTGAATTCAATAGTTCCTGGCTCGCTCTTGCAACATC 6 0.15 No Hit AATCACCCCTGCCTTGGTCATTTCTTTGCTGAACTCTTGCATCGTAACAG 6 0.15 No Hit CAAGATTATCCATTTTTAAACTGTATTGTTATCCTTACATCTCATGAGCT 6 0.15 No Hit CCTGTTTGTCTATCTGCACTGACAATAGGAGGCATGCCAGAACTAGTTCC 6 0.15 No Hit GTTTTTATTAAATTAAGTTCAACAGTTTTTCTCCAACTGCCTTCAACATG 6 0.15 No Hit CAGAAAAATTATGTTTAGGAGAACGCACAGAAAAATCAACTCCGGTACAG 6 0.15 No Hit CCCACCTATAATGTTCGCGATTCTCAGCGTCTCATTAACAACGCATTGAG 6 0.15 No Hit GGTGGGTTAGCTTTGTTGTCACTATAGAGAAATGGGATTGTGCTGTTTCA 6 0.15 No Hit CCACCGGCCCTAGCAGTAGTACTAGGCATTGCAGGCGCGATAAGCGCCTC 5 0.125 No Hit GGTCGATAGTTTTGTGTTATCTATGTCTTCCATAACCTTGGCGGCCGTAG 5 0.125 No Hit GTTAGAGGTTTCCTCATCAATCTCGCATCCAAATCCAAAATACCTCTCAC 5 0.125 No Hit GTGGGCTATGCGTTGTCTGCTCATAATCTTCCACTATATTCACTTGAATC 5 0.125 No Hit GGTCTATCATAACATTGTGAGGCTTGACATCTCTATGCATTATTCCTTGC 5 0.125 No Hit ATTATTATAATCCGTTTCTGGGTTGCTCAAGTAACTAAAATCCTGGCTTA 5 0.125 No Hit GCGGTGTTCTTGATGTTTTCCTTGAGTAGTTCCTCCTCATCTCCTCTGCC 5 0.125 No Hit GCCAGGGACTGTCACAAGAAGGGTACGGTATGCCTGGCGGTTAGCCTCGG 5 0.125 No Hit CGCTTGAGAAGGTGTGAGGCCTGTTTAACACCTCCAGTGTCGTTGATGGC 5 0.125 No Hit CTGGCTTCTTGCTGAGACCAAAAGGATCGTAACCATAGTCTCCAGGGACT 5 0.125 No Hit CTCCATTATCAGCAGGCAGATCGACAAGAACAAGAAAAGTATGTTACTGG 5 0.125 No Hit GCCTTGACTTGTTTGGCATAGGCCTTGAACCCAGGGGTTTGGACCTGTTT 5 0.125 No Hit GGGGTATGCTGCAGATATGTGATCAGAACAAGGAATGCATTCACCACAAG 5 0.125 No Hit CCCTTACCTCTGCAATATACCCCAAGAATTCAACACAGAGTGGCTTTCTG 5 0.125 No Hit CAAGAAAGACACAGTTCGATCTCCAGGAGACAATGTGTTGGTAAGTGCAC 5 0.125 No Hit GTTGGCATTGCTTCAATCAAGTTCTGATCCAGCAGAACCTTGTTGCTCCC 5 0.125 No Hit GAAACAGAGTAAAGAAGAAACAGGGGAGGTGAAAAAAAAAGAAGAAAAAG 5 0.125 No Hit GTCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTA 5 0.125 No Hit CTTCATCTTCTAAACCACCCAACTCCACTTCTTCCACAGCATGTTGACCA 5 0.125 No Hit CTCCTTTTCAACTTCCTTCGGATTCCTCATCCAAATCGGTTTTGTCTCAT 5 0.125 No Hit CAGAAAGAGCAGAAAAGGTGTTGGAATCCACTTCACATTCTCACCAAAGA 5 0.125 No Hit CCACACCAGAGGCCACAGACATGGCCAATACATAACAATGAAGAAGACAC 5 0.125 No Hit GTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTT 5 0.125 No Hit AGCTCATTTGCAAAGACCTTTACAAGCTCCCCCTTGCGCTTCCTGTCATC 5 0.125 No Hit CTCCCCAGGAAAATGTGTCAATGCTTGCTGTTTCATGCTTTCCTGTCAAC 5 0.125 No Hit GCTTCACTAAAATCCTCACAGGTTTATTCATGAACTTCCTCGTAATTTCT 5 0.125 No Hit TTCCTCATACTTCGAGGAACAGAAGGCATTAAATTGCCACGGCTCCCCTG 5 0.125 No Hit GTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACC 5 0.125 No Hit CTGCCTTGAAAAATTCACATAGTATAGACTTGGGATCGACACCAACTGGC 5 0.125 No Hit CCACGTTGGGAAGTGCCCGTAGGCTTGTCACTGGCACCCGGCGAGTAAAC 5 0.125 No Hit CATCCAAGAACACTGGAACTCGTCCTTGTGCAGCCTTGACAACCTCTTCC 5 0.125 No Hit CTGCAATCTATTAAAGGTCTAGTCATGAAGCCAATGGTAGAATTTGAGTC 5 0.125 No Hit CAATTCAAATATGTGCTGACTTAGGGCCTCATGTCTTTGCATGGCCACTG 5 0.125 No Hit CTTCGTTACTCACAACCACCCATGACTTGTAGCGAGGAGACTGCGGAATA 5 0.125 No Hit ATCCTGTGTAACCTCTTAAATAGAAACTTGTATCAGAAGGCAACAAAGTC 5 0.125 No Hit GCCCTTGTCGCCTATACCTTCCACCGGATGCTAAAAATCCAGGACAGGGC 5 0.125 No Hit ACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCG 5 0.125 No Hit CCGGTATTCAGTTTCTTCAGGGGTGAAGGAGGAAGGGGGCTTAGCCTGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.125 0.0 0.0 0.0 0.0 88-89 0.21250000000000002 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.44999999999999996 0.0 0.0 0.0 0.0 96-97 0.6 0.0 0.0 0.0 0.0 98-99 0.7875 0.0 0.0 0.0 0.0 100-101 0.9375 0.0 0.0 0.0 0.0 102-103 1.1625 0.0 0.0 0.0 0.0 104-105 1.5 0.0 0.0 0.0 0.0 106-107 1.775 0.0 0.0 0.0 0.0 108-109 2.1875 0.0 0.0 0.0 0.0 110-111 2.35 0.0 0.0 0.0 0.0 112-113 2.625 0.0 0.0 0.0 0.0 114-115 2.875 0.0 0.0 0.0 0.0 116-117 3.05 0.0 0.0 0.0 0.0 118-119 3.55 0.0 0.0 0.0 0.0 120-121 4.225 0.0 0.0 0.0 0.0 122-123 4.5375 0.0 0.0 0.0 0.0 124-125 4.9375 0.0 0.0 0.0 0.0 126-127 5.3625 0.0 0.0 0.0 0.0 128-129 6.1625 0.0 0.0 0.0 0.0 130-131 6.6875 0.0 0.0 0.0 0.0 132-133 7.375 0.0 0.0 0.0 0.0 134-135 7.862500000000001 0.0 0.0 0.0 0.0 136-137 8.350000000000001 0.0 0.0 0.0 0.0 138-139 8.85 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGCC 10 0.006830828 145.0 6 AATCTAT 10 0.006830828 145.0 5 ACTTTTT 10 0.006830828 145.0 3 TAACTTT 10 0.006830828 145.0 1 TGCCATC 10 0.006830828 145.0 9 TGCTTCT 10 0.006830828 145.0 145 >>END_MODULE SRR13695406 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695406_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0925 37.0 37.0 37.0 37.0 37.0 2 36.187 37.0 37.0 37.0 37.0 37.0 3 36.134 37.0 37.0 37.0 37.0 37.0 4 36.122 37.0 37.0 37.0 37.0 37.0 5 36.2745 37.0 37.0 37.0 37.0 37.0 6 36.3165 37.0 37.0 37.0 37.0 37.0 7 36.148 37.0 37.0 37.0 37.0 37.0 8 36.2475 37.0 37.0 37.0 37.0 37.0 9 36.2365 37.0 37.0 37.0 37.0 37.0 10-14 36.2801 37.0 37.0 37.0 37.0 37.0 15-19 36.25 37.0 37.0 37.0 37.0 37.0 20-24 36.26435 37.0 37.0 37.0 37.0 37.0 25-29 36.14585 37.0 37.0 37.0 37.0 37.0 30-34 36.108549999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.075849999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.085750000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.02675000000001 37.0 37.0 37.0 37.0 37.0 50-54 35.928450000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.972049999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.98975 37.0 37.0 37.0 37.0 37.0 65-69 35.88680000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.85695 37.0 37.0 37.0 37.0 37.0 75-79 35.896750000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.878550000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.78655 37.0 37.0 37.0 37.0 37.0 90-94 35.730450000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.73505 37.0 37.0 37.0 37.0 37.0 100-104 35.68825 37.0 37.0 37.0 37.0 37.0 105-109 35.67575000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.65545 37.0 37.0 37.0 37.0 37.0 115-119 35.53485 37.0 37.0 37.0 37.0 37.0 120-124 35.561350000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.54105 37.0 37.0 37.0 37.0 37.0 130-134 35.488350000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.281150000000004 37.0 37.0 37.0 32.2 37.0 140-144 35.21485 37.0 37.0 37.0 32.2 37.0 145-149 34.98595 37.0 37.0 37.0 25.0 37.0 150-151 34.83825 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.0 15 0.0 16 0.0 17 2.0 18 3.0 19 1.0 20 0.0 21 0.0 22 2.0 23 2.0 24 13.0 25 10.0 26 10.0 27 11.0 28 11.0 29 21.0 30 28.0 31 35.0 32 74.0 33 115.0 34 257.0 35 594.0 36 2600.0 37 207.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.44204716507777 19.719016557952834 13.020572002007025 33.81836427496237 2 25.825 25.2 34.25 14.725 3 20.875 26.900000000000002 32.75 19.475 4 22.725 33.45 24.025 19.8 5 25.174999999999997 35.875 22.3 16.650000000000002 6 19.025 41.675000000000004 22.675 16.625 7 20.05 22.650000000000002 37.925 19.375 8 20.8 25.674999999999997 29.95 23.575 9 19.475 24.474999999999998 31.525 24.525 10-14 22.125 29.404999999999998 27.235 21.235 15-19 21.985 29.715000000000003 27.43 20.87 20-24 22.40060015003751 28.232058014503625 28.747186796699175 20.62015503875969 25-29 21.11027756939235 28.80720180045011 28.57214303575894 21.510377594398598 30-34 21.510377594398598 28.987246811702928 27.941985496374095 21.560390097524383 35-39 22.040510127531885 28.197049262315577 28.1470367591898 21.615403850962743 40-44 21.69542385596399 29.067266816704173 28.9472368092023 20.29007251812953 45-49 21.990497624406103 28.927231807951987 28.587146786696678 20.49512378094524 50-54 21.72043010752688 28.73718429607402 29.202300575143784 20.340085021255312 55-59 22.165541385346337 27.861965491372843 28.877219304826205 21.09527381845461 60-64 22.565641410352587 29.0622655663916 27.44186046511628 20.930232558139537 65-69 22.749549909981997 28.845769153830762 27.880576115223043 20.524104820964194 70-74 22.735683920980247 28.497124281070267 27.521880470117527 21.24531132783196 75-79 22.975743935983996 28.1470367591898 28.047011752938232 20.830207551887973 80-84 22.315578894723682 28.55213803450863 27.926981745436358 21.205301325331334 85-89 22.875718929732432 27.28182045511378 28.667166791697923 21.175293823455867 90-94 22.545636409102276 28.13703425856464 28.992248062015502 20.32508127031758 95-99 23.090772693173292 27.611902975743934 27.991997999499873 21.305326331582897 100-104 24.051012753188296 27.71692923230808 27.95198799699925 20.280070017504375 105-109 23.6609152288072 28.33208302075519 27.6419104776194 20.365091272818205 110-114 23.565891472868216 28.787196799199798 27.89197299324831 19.754938734683673 115-119 24.461115278819705 27.671917979494875 27.95198799699925 19.91497874468617 120-124 23.72093023255814 27.84196049012253 28.11702925731433 20.320080020005 125-129 24.781195298824706 27.7569392348087 26.70667666916729 20.7551887971993 130-134 24.921230307576895 28.502125531382845 26.966741685421354 19.609902475618902 135-139 24.921230307576895 28.267066766691674 27.291822955738937 19.5198799699925 140-144 26.486621655413856 27.176794198549636 27.301825456364092 19.03475868967242 145-149 25.481370342585645 27.516879219804952 27.631907976994246 19.369842460615153 150-151 26.419104776194047 28.14453613403351 26.84421105276319 18.592148037009252 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.5 20 2.5 21 2.5 22 0.5 23 2.0 24 5.5 25 11.5 26 12.0 27 10.5 28 15.0 29 15.5 30 16.0 31 29.5 32 48.5 33 53.0 34 58.5 35 98.0 36 114.0 37 125.0 38 164.0 39 191.0 40 207.0 41 225.0 42 260.0 43 261.0 44 244.0 45 247.0 46 246.5 47 236.0 48 210.0 49 171.0 50 142.5 51 122.0 52 95.0 53 77.0 54 69.5 55 54.0 56 41.0 57 35.0 58 23.5 59 16.0 60 13.5 61 8.0 62 3.5 63 3.0 64 1.5 65 0.5 66 0.5 67 1.0 68 1.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.02 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.025 135-139 0.025 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.349999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 66.41673243883189 42.075 2 20.086819258089978 25.45 3 7.300710339384373 13.875000000000002 4 3.591160220994475 9.1 5 1.22336227308603 3.875 6 0.9865824782951855 3.75 7 0.2762430939226519 1.225 8 0.03946329913180742 0.2 9 0.07892659826361484 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGGAAGCTTTAAGCATGTAATATTGGCTCCTTGTGTCATTCACAACATCT 9 0.22499999999999998 No Hit AGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 9 0.22499999999999998 No Hit GGAGAAAGCAGAGATTGCTGGTACACCACAGAAGCAAGATTCTGCTTTGA 8 0.2 No Hit ATGGCAAGACAACCCTATTGGATCATATTCGTAAAAGCAAGGTGGCTGCC 7 0.17500000000000002 No Hit ATTGCCTTCAAATGAAGCTTCGATGCTTTTAACTTCTAATCCAATTTATT 7 0.17500000000000002 No Hit ATCAATGGAGCGTACTGCTTTATCACTAGGACAGGATACACTGGAGAAGA 7 0.17500000000000002 No Hit GGTAGTTTCCGTCTGTACAAGGAAGGAGTTTACACCACCAGCACTTGTGG 7 0.17500000000000002 No Hit CTTTGAGAGCAACCCAATTCTCCGGATCCCACCTAACGTTGAAAAAGATT 7 0.17500000000000002 No Hit CAACAATACGACGGCGTTTGCCTCTAGTCGGGGCGTTTTGCATGATAAGC 7 0.17500000000000002 No Hit TGCAAATTTAGCACCTTCAGATGTGAATTCAAGAGGGGAAAATTGGGATT 7 0.17500000000000002 No Hit ATTGAAGACTGTGTGAGAGCTGGTTGCTATGCAGCAAATGTCATTATCCA 6 0.15 No Hit GTCGAAACAGATTTCAGAACCGGAGATGATCCATGGTGGACTTAAGTCAC 6 0.15 No Hit GTGATGGCCTTGTTCAATGAAGAACCATGCATCTACACTGCTGATATTAA 6 0.15 No Hit GGAGCATGAACAGATTAGAGCAAAGAAGGGTGAGGGGGAAGCTCTTGAGT 6 0.15 No Hit ATGTAGTGAAGCTTCTAGACATTGTTAGAGATCAGCACTCAAAAACTCCT 6 0.15 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit AAAAAAGAAAGATGGCCTCGGCATCATTTCTCAAGTCATCACCAGTTCTA 6 0.15 No Hit GACAGCTTATGTCTCACCTAGTTCAAGAGTTTTTGGGAGAGGTGAACTTG 6 0.15 No Hit GTTTGACATGTTGCGGAGACAATCACTTCGCCCTGACTATATCAAGATGT 6 0.15 No Hit TGAAGGTGACATGAGCAGTGCTGAGCATCAAGAAGTGATTTCTAATGACC 6 0.15 No Hit GTTGAAGGTGTTCGGACTTTCAAGAATACATTGTATTGTAAGAAGGCTGT 6 0.15 No Hit GTCTAGAAGAACAGTAAACCGTCTTTATGAAAATAAGGCACAGATGAATT 6 0.15 No Hit CTGCATAGCTTACAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 6 0.15 No Hit CAAGGAGACCATAAGTATACATGATATTACCTTCGTTCGACAAGTGTCTT 6 0.15 No Hit GCTTTCCCAGTCAGTACCAGAAAGGCTAATGACATTACTTCCATTGCAAG 6 0.15 No Hit GATCTCGGTTTCACTGGTATCATGCTGTTTTTGCCGTAGGACTATTGGCA 6 0.15 No Hit CATCAGATCACTACCTTAGAACCATAAACGCGGATTTTGATATGGACATT 6 0.15 No Hit GTGGTAGTAGGGGCACGCAGACACAAAAACACAGAAAGAAGAAGAAGAAG 6 0.15 No Hit CGTGGTTATCCAACTGAATTTGCCTCTTACTTTCATTATTGTCGTTCACT 6 0.15 No Hit CCCGTTCTTCCCCTTAACATCGAGGCAACTAACCATGAAGATGCTGTGAA 6 0.15 No Hit ATGGGAATCGATCCAACAGAGCCACAGGCTCTGGCTACTGGAAGGCAACT 6 0.15 No Hit AGGAGGCATTGCTGCAGTAGATAAATTCAGTGGGAAACCAACTGAGGCTA 6 0.15 No Hit TTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATC 6 0.15 No Hit GCTTTATTGACTACCTTTCCCTTCACTTTTGCCTCTTCAAAGAGAAGTTT 6 0.15 No Hit GGGAACATGACCGTGATGGTGAAAGGGAGAGGGAGAGAGAGAAGGAGACG 6 0.15 No Hit GGTCTATCTAGACATTGTTAGAGATCAGCACTCAAAAACTCCTAGCCTCA 5 0.125 No Hit ATTCACTGATTTCTCAGGTCTCCGCAGCTCATCAGCTTTCCTTCCCTTTA 5 0.125 No Hit GTCCCGTCTCACTAACCCGTCATTCAAACCTTCACCTTCTTTCTTCTTTT 5 0.125 No Hit GAACCATCAAGTTTGAGGAGAAAGATGGTATTGACTATGCTGCTGTGACC 5 0.125 No Hit GACTTTGAATTGGTGGGGTACAAATGGTGAACACTGTGAATTGGCAACTT 5 0.125 No Hit CTCCAATCTATCAACAAAACCACTAACCCCGCCGCCCGTTTCACTTCCAT 5 0.125 No Hit GTGGTGCTCTTTTGCTTTGTGATATGGCTCACATCAGTGGCCTTGTTGCT 5 0.125 No Hit GAAAGAGTTCCCAAAGGGTTTGCTCTTATTTATTATCGTGGGAAGAATTA 5 0.125 No Hit CGGGCAATGAATAGGAACAACGAGGATTACCGTTCACCAGCAGTTCCAGC 5 0.125 No Hit ATTGTTATTTCTTTCTTTTTCGCTCGTGGTTTTGTTAAGACTGGATTAGG 5 0.125 No Hit CAGAAAGCTTTGCAACAAAGAGATATGGCTTCCCTGGCAGCATCCAGAGC 5 0.125 No Hit GATAGGTTTGCTTGCCACTACGATCCATATGGCCCTATCTACAATGATCG 5 0.125 No Hit GTTGTGAATGCTCCCGGGGTCACTGCTGATATTAGTCACATGGACACTGG 5 0.125 No Hit CACCAAACTGCCAATTCTAGTGAAGGGTGTACTCACTGCTGAGGATGCAA 5 0.125 No Hit CTTCAAACATCTCAATTTCCTCTTCATCTCATCAGTCTCACATCAATCCC 5 0.125 No Hit CGACAAGCTTCCTCTCAGTGGTTGTAAGGGCTGTGGGAGAGAGGAAATAG 5 0.125 No Hit GGGCCTCCAAATTTCAGGCGAAGCACTTCCAGGAAGAGAACTCCAGGCAT 5 0.125 No Hit GCTAGCTAGAAGTCACTCTACCCTTCGCATTACTTCCTCAATCAACCACT 5 0.125 No Hit CGGGAGAGCATACTAGGGATGTTAAAGAACAGGAAATTGGCCTGGAGTTC 5 0.125 No Hit CATTGATCTTGAAGACCACGCTGAGGAATTGATCGTTTCTCTTGATTCAT 5 0.125 No Hit AGAAGAAAGTGAATGCAAATGCTATTCCTCATGATAAAGATTCTAGAATA 5 0.125 No Hit GTTCATCAAAGAAAAAAAAGATATAACACCCTATTAGGCGAATAAAGACT 5 0.125 No Hit TTTTCTAATTTGACACTGCTTTAAATTTTCCTCATTTCGCTGCTAAAAAT 5 0.125 No Hit CTTCATTAAAACCACACCAGAGGCCACAGACATGGCCAATACATAACAAT 5 0.125 No Hit CACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 5 0.125 No Hit CTTGTTGAAAGGTTACATCGAAGAATCTGAAAGGTGGGAAAGAAACGAAG 5 0.125 No Hit GTGGTTTGGTACGGAAAAATGGGTATCATAGTTCAACGATTGTGCCTAGC 5 0.125 No Hit TCAAGATACATTCTAGAGAGAATCACTGAACAAGCTGGTGTCGTGCTCTC 5 0.125 No Hit CGGAGAAGAAGCAAGACATTATTGTTATTGCTATAACATAAAATCATATA 5 0.125 No Hit GGCAACAATTTTTTAAAAAATTCTTTGAAAATCCCTCTTTTTTGCTCGAC 5 0.125 No Hit TGGAAGAGGAGGAAGAACCAAAAGAAGGAGAAGAGGTGCCAGAGGGTGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.125 0.0 0.0 0.0 0.0 88-89 0.21250000000000002 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.44999999999999996 0.0 0.0 0.0 0.0 96-97 0.6 0.0 0.0 0.0 0.0 98-99 0.7875 0.0 0.0 0.0 0.0 100-101 0.9375 0.0 0.0 0.0 0.0 102-103 1.1625 0.0 0.0 0.0 0.0 104-105 1.475 0.0 0.0 0.0 0.0 106-107 1.75 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.325 0.0 0.0 0.0 0.0 112-113 2.5999999999999996 0.0 0.0 0.0 0.0 114-115 2.8499999999999996 0.0 0.0 0.0 0.0 116-117 3.0250000000000004 0.0 0.0 0.0 0.0 118-119 3.525 0.0 0.0 0.0 0.0 120-121 4.2 0.0 0.0 0.0 0.0 122-123 4.5125 0.0 0.0 0.0 0.0 124-125 4.949999999999999 0.0 0.0 0.0 0.0 126-127 5.4 0.0 0.0 0.0 0.0 128-129 6.2125 0.0 0.0 0.0 0.0 130-131 6.737500000000001 0.0 0.0 0.0 0.0 132-133 7.45 0.0 0.0 0.0 0.0 134-135 7.9375 0.0 0.0 0.0 0.0 136-137 8.4375 0.0 0.0 0.0 0.0 138-139 8.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGAGA 10 0.006830828 145.0 8 GGAGGGA 10 0.006830828 145.0 145 GAGGATT 20 0.00593511 29.0 55-59 AGGATTT 20 0.00593511 29.0 55-59 >>END_MODULE Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1002003 spots for SRR13695406.sra Written 1002003 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra Read 1001992 spots for SRR13695406.sra Written 1001992 spots for SRR13695406.sra SRR ids: ['SRR13695406.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4xyzm38f SRR13695406.sra spots: 20039851 blocks: [[1, 1001992], [1001993, 2003984], [2003985, 3005976], [3005977, 4007968], [4007969, 5009960], [5009961, 6011952], [6011953, 7013944], [7013945, 8015936], [8015937, 9017928], [9017929, 10019920], [10019921, 11021912], [11021913, 12023904], [12023905, 13025896], [13025897, 14027888], [14027889, 15029880], [15029881, 16031872], [16031873, 17033864], [17033865, 18035856], [18035857, 19037848], [19037849, 20039851]] SRR13695406 file size 6788717 SRR13695406 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695406 SRR13695406_1.fastq SRR13695406_2.fastq Input file: SRR13695406_1.fastq Paired file: SRR13695406_2.fastq trimmed: SRR13695406-trimmed-pair1.fastq, SRR13695406-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:03:11 2025 >> started Wed Feb 12 01:03:34 2025 >> done (22.556s) 20039851 read pairs processed; of these: 138 ( 0.00%) short read pairs filtered out after trimming by size control 1022 ( 0.01%) empty read pairs filtered out after trimming by size control 20038691 (99.99%) read pairs available; of these: 2462364 (12.29%) trimmed read pairs available after processing 17576327 (87.71%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 1 0.00% 20 3 0.00% 21 2 0.00% 22 3 0.00% 23 2 0.00% 24 0 0.00% 25 5 0.00% 26 1 0.00% 27 1 0.00% 28 6 0.00% 29 4 0.00% 30 2 0.00% 31 3 0.00% 32 9 0.00% 33 2 0.00% 34 4 0.00% 35 6 0.00% 36 9 0.00% 37 8 0.00% 38 12 0.00% 39 23 0.00% 40 10 0.00% 41 16 0.00% 42 14 0.00% 43 17 0.00% 44 21 0.00% 45 21 0.00% 46 27 0.00% 47 35 0.00% 48 34 0.00% 49 58 0.00% 50 59 0.00% 51 70 0.00% 52 69 0.00% 53 81 0.00% 54 95 0.00% 55 114 0.00% 56 143 0.00% 57 156 0.00% 58 177 0.00% 59 207 0.00% 60 257 0.00% 61 279 0.00% 62 326 0.00% 63 351 0.00% 64 417 0.00% 65 427 0.00% 66 558 0.00% 67 648 0.00% 68 741 0.00% 69 909 0.00% 70 945 0.00% 71 1190 0.01% 72 1406 0.01% 73 1499 0.01% 74 1788 0.01% 75 2022 0.01% 76 2319 0.01% 77 2454 0.01% 78 2784 0.01% 79 3293 0.02% 80 3556 0.02% 81 3975 0.02% 82 4290 0.02% 83 5053 0.03% 84 5786 0.03% 85 6298 0.03% 86 6847 0.03% 87 7458 0.04% 88 8116 0.04% 89 8490 0.04% 90 9400 0.05% 91 10096 0.05% 92 10773 0.05% 93 11869 0.06% 94 12813 0.06% 95 14318 0.07% 96 14846 0.07% 97 15789 0.08% 98 16178 0.08% 99 17321 0.09% 100 18517 0.09% 101 19228 0.10% 102 19960 0.10% 103 21543 0.11% 104 22223 0.11% 105 23517 0.12% 106 25013 0.12% 107 25885 0.13% 108 26512 0.13% 109 27554 0.14% 110 27829 0.14% 111 29410 0.15% 112 30141 0.15% 113 31322 0.16% 114 32833 0.16% 115 34524 0.17% 116 35316 0.18% 117 36786 0.18% 118 38028 0.19% 119 38334 0.19% 120 39733 0.20% 121 40515 0.20% 122 41855 0.21% 123 42356 0.21% 124 43840 0.22% 125 44554 0.22% 126 46086 0.23% 127 47657 0.24% 128 48321 0.24% 129 48954 0.24% 130 50137 0.25% 131 50276 0.25% 132 50467 0.25% 133 52434 0.26% 134 52927 0.26% 135 54682 0.27% 136 55077 0.27% 137 55791 0.28% 138 57025 0.28% 139 58792 0.29% 140 59076 0.29% 141 60678 0.30% 142 60114 0.30% 143 60591 0.30% 144 62764 0.31% 145 63174 0.32% 146 63882 0.32% 147 64825 0.32% 148 66616 0.33% 149 67147 0.34% 150 68124 0.34% 151 17576327 87.71% 20038691 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.31 fanout-score-rank=28 prefix-density=0.29 prefix-fanout=2.2 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=560.93 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=20.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=sequence-density sequence-density=0.75 sequence-density-rank=1 fanout-score=2.29 fanout-score-rank=28 prefix-density=0.77 prefix-fanout=2.2 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.07 sequence-density-rank=27 fanout-score=64.64 fanout-score-rank=1 prefix-density=0.47 prefix-fanout=9.9 sequence=AGAAAAGAAAACAGATTATCAAGCTTACTAGAATTATGGAAGGAATGAGTGTGGAGAACATGCACAAGATAGTGGTGGCAGTGGATGAGAGTGAGGAGAGCATGCATGCTCTTTCATGGTGTCTCAGCAACCTTATTTCTCACAACTCCACCGCCACGTTAGTCCTCCTCTAT SRR13695406 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:04:13 Started mapping on | Feb 12 01:04:14 Finished on | Feb 12 01:06:15 Mapping speed, Million of reads per hour | 596.19 Number of input reads | 20038691 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 19008761 Uniquely mapped reads % | 94.86% Average mapped length | 294.51 Number of splices: Total | 18558900 Number of splices: Annotated (sjdb) | 18132131 Number of splices: GT/AG | 18183725 Number of splices: GC/AG | 286319 Number of splices: AT/AC | 11076 Number of splices: Non-canonical | 77780 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 459868 % of reads mapped to multiple loci | 2.29% Number of reads mapped to too many loci | 81716 % of reads mapped to too many loci | 0.41% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.34% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 570281 570281 570281 N_multimapping 459868 459868 459868 N_noFeature 816471 18579207 1076989 N_ambiguous 286986 1753 116698 UnstrandedReadsAssigned:17905304 PositiveStrandReadsAssigned:427801 NegativeStrandReadsAssigned:17815074 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695406 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695406-trimmed-pair1.fastq SRR13695406-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,038,691 reads, 17,804,426 reads pseudoaligned [quant] estimated average fragment length: 248.381 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,143 rounds 52401 SRR13695406.ke.tsv 34699 SRR13695406.se.tsv 87100 total ==> SRR13695406.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1770.62 753 21.8138 Potri.005G024800.1.v4.1 1035 787.619 378 24.6171 Potri.004G059700.1.v4.1 961 713.777 16 1.14979 Potri.007G009000.2.v4.1 1416 1168.62 0 0 Potri.003G141000.2.v4.1 2943 2695.62 954.832 18.169 Potri.016G087400.1.v4.1 270 86.6928 880 520.668 Potri.015G069301.1.v4.1 564 326.645 0 0 Potri.010G195200.1.v4.1 1773 1525.62 323 10.8597 Potri.012G127500.1.v4.1 977 729.697 94 6.60766 ==> SRR13695406.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 360 Potri.001G233950.v4.1 3 Potri.001G122700.v4.1 313 Potri.001G212900.v4.1 25 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 26 Potri.001G416900.v4.1 3 Potri.001G452600.v4.1 2 SRR13695406 completed mapping pipeline successfully