Starting /dee2/code/volunteer_pipeline.sh SRR13695407 current disk space = 3051256700928 free memory = 1437935444 SRR13695407 SRAfilesize 3daf12aedb4ec5fd04040ee8dfb95201 SRR13695407.sra SRR13695407.sra file validated SRR13695407 is paired end SRR13695407 is conventional basespace SRR13695407 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695407_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5565 37.0 37.0 37.0 37.0 37.0 2 36.42775 37.0 37.0 37.0 37.0 37.0 3 36.5115 37.0 37.0 37.0 37.0 37.0 4 36.5845 37.0 37.0 37.0 37.0 37.0 5 36.596 37.0 37.0 37.0 37.0 37.0 6 36.5085 37.0 37.0 37.0 37.0 37.0 7 36.545 37.0 37.0 37.0 37.0 37.0 8 36.581 37.0 37.0 37.0 37.0 37.0 9 36.5245 37.0 37.0 37.0 37.0 37.0 10-14 36.5295 37.0 37.0 37.0 37.0 37.0 15-19 36.5247 37.0 37.0 37.0 37.0 37.0 20-24 36.474599999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.441199999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.4187 37.0 37.0 37.0 37.0 37.0 35-39 36.4036 37.0 37.0 37.0 37.0 37.0 40-44 36.3719 37.0 37.0 37.0 37.0 37.0 45-49 36.366499999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.3123 37.0 37.0 37.0 37.0 37.0 55-59 36.3566 37.0 37.0 37.0 37.0 37.0 60-64 36.318200000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.245400000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.2532 37.0 37.0 37.0 37.0 37.0 75-79 36.245000000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.241299999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.1969 37.0 37.0 37.0 37.0 37.0 90-94 36.1642 37.0 37.0 37.0 37.0 37.0 95-99 36.122 37.0 37.0 37.0 37.0 37.0 100-104 36.1015 37.0 37.0 37.0 37.0 37.0 105-109 36.120000000000005 37.0 37.0 37.0 37.0 37.0 110-114 36.076299999999996 37.0 37.0 37.0 37.0 37.0 115-119 36.080400000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.973400000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.9432 37.0 37.0 37.0 37.0 37.0 130-134 35.9283 37.0 37.0 37.0 37.0 37.0 135-139 35.8855 37.0 37.0 37.0 37.0 37.0 140-144 35.7352 37.0 37.0 37.0 37.0 37.0 145-149 35.656 37.0 37.0 37.0 37.0 37.0 150-151 35.480999999999995 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 0.0 19 0.0 20 1.0 21 0.0 22 2.0 23 3.0 24 4.0 25 3.0 26 9.0 27 8.0 28 21.0 29 13.0 30 30.0 31 39.0 32 44.0 33 75.0 34 102.0 35 313.0 36 2894.0 37 437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.425000000000004 12.075 9.525 40.975 2 19.47302383939774 13.500627352572145 35.708908406524465 31.317440401505646 3 19.275000000000002 17.224999999999998 27.125 36.375 4 20.325 24.425 23.974999999999998 31.275 5 24.5 29.525000000000002 24.7 21.275 6 20.625 32.375 24.4 22.6 7 16.275000000000002 28.050000000000004 42.0 13.675 8 18.45 25.775 32.824999999999996 22.95 9 17.4 23.599999999999998 36.325 22.675 10-14 19.54 30.335 27.875 22.25 15-19 18.884999999999998 28.89 27.97 24.255 20-24 20.865000000000002 28.655 27.85 22.63 25-29 20.205000000000002 27.96 27.634999999999998 24.2 30-34 19.63 28.505000000000003 27.985 23.880000000000003 35-39 20.015 29.23 27.389999999999997 23.365 40-44 20.71 28.860000000000003 26.950000000000003 23.48 45-49 19.875 28.78 28.065 23.28 50-54 19.919999999999998 30.39 26.525 23.165 55-59 20.085 29.175 27.045 23.695 60-64 20.155 29.365000000000002 27.455000000000002 23.025000000000002 65-69 20.59 28.975 26.995 23.44 70-74 20.215 27.785 27.975 24.025 75-79 20.22 28.335 27.47 23.974999999999998 80-84 20.560000000000002 28.575 26.965 23.9 85-89 20.635 27.639999999999997 27.925 23.799999999999997 90-94 20.41 28.02 27.339999999999996 24.23 95-99 20.715 28.065 27.139999999999997 24.08 100-104 19.950000000000003 29.654999999999998 27.24 23.155 105-109 20.525 27.96 28.084999999999997 23.43 110-114 20.01 27.87 28.27 23.849999999999998 115-119 21.154999999999998 28.060000000000002 26.889999999999997 23.895 120-124 20.685000000000002 28.235 26.974999999999998 24.104999999999997 125-129 21.3 27.62 27.245 23.835 130-134 22.06 27.715 26.565 23.66 135-139 21.46 28.720000000000002 26.105 23.715 140-144 20.810000000000002 27.750000000000004 27.46 23.98 145-149 21.335 27.975 26.75 23.94 150-151 21.975 26.8625 27.537499999999998 23.625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 2.0 23 2.0 24 1.5 25 2.0 26 2.0 27 8.5 28 15.5 29 14.0 30 12.0 31 14.0 32 30.0 33 50.5 34 67.5 35 89.0 36 105.5 37 118.0 38 142.0 39 146.0 40 169.0 41 202.0 42 214.0 43 239.0 44 248.0 45 258.0 46 255.5 47 254.0 48 253.5 49 214.5 50 174.5 51 155.5 52 128.5 53 91.5 54 74.5 55 73.0 56 61.5 57 37.5 58 17.0 59 18.0 60 19.5 61 8.0 62 2.0 63 2.0 64 2.5 65 1.0 66 2.0 67 2.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.375 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.825 #Duplication Level Percentage of deduplicated Percentage of total 1 70.5860670844084 47.875 2 18.54036122373756 25.15 3 6.671581275340952 13.575000000000001 4 2.5064504238849983 6.800000000000001 5 1.0320678215997052 3.5000000000000004 6 0.36859565057132326 1.5 7 0.11057869517139698 0.525 8 0.07371913011426465 0.4 9 0.11057869517139698 0.675 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCACCAAGCCAAGCACCACCACCAGGGCTAACAGATTGTTCAACAATGGC 9 0.22499999999999998 No Hit GCACTCATCATGATTGGGCTAATTCCCATCTTGGCAAAGACCAGTTCACA 9 0.22499999999999998 No Hit GCCAGCGGATCGCTCTCATTTACAAGTGCAAGGATCGCAGGTACAGTTGG 9 0.22499999999999998 No Hit AAATTATTACAAACATAATTTAATATCTCCTTCGCAAAGCAGCACTGTAC 8 0.2 No Hit CATAGCTTCGATCAAAGTCGGGGTCCTTACGCTCAACCTCACGGTCCCGA 8 0.2 No Hit GTTTTCAGTATGCTTTACAGCAGGATTTGTAATGTTGGATGCTGCTACTA 7 0.17500000000000002 No Hit CTGCACTCTTCACATCAATATCAAGGAAATCTTTCTCAGCGTTGTCTTTG 7 0.17500000000000002 No Hit GTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACC 7 0.17500000000000002 No Hit GTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTT 6 0.15 No Hit ATCGGCATTACATCAAAGCGACAACCTATTTAACCCCTTTCTATGCCCTA 6 0.15 No Hit GTGACGCTGAACATACAATCATGGATCTTGGTTTTATTGGGGTCATTTGA 6 0.15 No Hit GGGACAACATTGACATGACAAATACAAGGCAATGACAATTGTAACTAATG 6 0.15 No Hit CTTTACTAGAGCTGCCCAGTTCCAGTTCGTCTGCAAGGTCCTCGCATTAC 6 0.15 No Hit CTGAGGGTCAAAGCTGCATTGTAGTAAAGAGCATCTGCAACCACTTGCAT 6 0.15 No Hit CTTCATTAAAACCACACCAGAGGCCACAGACATGGCCAATACATAACAAT 6 0.15 No Hit CTCTATTGATAAAGAGATCTTTGATTTGTTCTCAATGGTTGAGCCTTGGT 6 0.15 No Hit AATCACTCAGGAGCTGATTGCAAGTTGGCCGCACATATGTCTGAAAACAA 6 0.15 No Hit GGATGATTGTTTCCATGGAACAATGGTTTGGTTGCTTGATTTTAGGGTGG 6 0.15 No Hit CAACAATCTCTGACCAATAGTCACAAAACTAGTCTCCTGATTTGACATAA 5 0.125 No Hit GCCCCTTCACCTTGAATGGTGGTCCTGAGCCAATCACCAGCATCTTCCCA 5 0.125 No Hit CCCACACTGAATATAAATGCCTTGGTCAACAGTCCTCTATCAAAACCGCA 5 0.125 No Hit CTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCT 5 0.125 No Hit GTCTCTCATTCAAAAACACAACTCTTGCACTGTTGATAACATAAGTTTGA 5 0.125 No Hit CTGCACTATCAATGTATGGGGGAACAAGAACTGCAGTCACTGTGTCACTG 5 0.125 No Hit GGGAAGAGCATTGCATTCAACAATCTGATTCTTGTTCTCATCATTCTTAA 5 0.125 No Hit CTGTAATGCCAATAGTGGCCACCGGCCCTTCATGCTTCACCCACTCATGT 5 0.125 No Hit CGAATCTTTGCTCTCGTTATGTGATCTCAGACAAATATAAAGTAACTAAA 5 0.125 No Hit GATGAAGTTAGAAGCTTGTTTGCCGCTGTATCTCATCATGTTTGTCACTA 5 0.125 No Hit CCCAGATTTCCATTTTTCACTACTTTCGGAATCGGACTCCCTTTTGGAGC 5 0.125 No Hit GGAGTGGTGATGATGATTTCTCTTGTGTTGATGGTGTTTGTGACGCTGAA 5 0.125 No Hit CACCATTTCCACATTCTTTCGACAACGGCTCCCCACACAATCCTGGATTC 5 0.125 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5 0.125 No Hit CTGTAACAAGGCCTCCCCATCTGTCCTTTTCGGTCTGCTTCTCCTCATCG 5 0.125 No Hit GGTTTCTCCAAGTCAGTAACCTTGGTTGCCCCATCGGCATCCAGCATTAG 5 0.125 No Hit GCTTCATCTTGGGTGGCTCTTTCTGGGCCTTGAATCGGTAATCAGTGTCG 5 0.125 No Hit GCCATGGGTTTGGAGATTGGTTCATTGCGTTGAGGTTCAGGGTTGCTTCG 5 0.125 No Hit TTTTTTTTTTGACAAAGTTGGAAAATATTTTTTGTGATAATTGACAAGAA 5 0.125 No Hit TACAGGCATATGAGCTTACAGAGCTAGTTCGCTCTTCGGTTAAAATTGTA 5 0.125 No Hit CGCCAGCGTTTCTCACTTGGGGGTAAAAGCAGCAAAAACGGTGGCATGAC 5 0.125 No Hit GTACCAGTCAGGATCATATGGCGCAAGCTCCTTAAATTTAGCAGTCTTCA 5 0.125 No Hit GTTATTTCCTCAGTCATCTGTTTCTGCCCACAGAGAACAGCACCAGTGCC 5 0.125 No Hit TGGGTAGGGGAGAGAAGACGATGGTAGCTAAGAGAATGCATGCGATAAGA 5 0.125 No Hit ACTTCTTCTTCCTGCAGTTGACAGCACGAGGATGGAGGCGTGCATAACAT 5 0.125 No Hit CCTTGGGAAGCATCCAAGCCAGCATTTGAGATAGCTTTAGGTATGGCGAG 5 0.125 No Hit GAAGGATTCATCTGAGTCTTTTATTGAAACAAAAGAGGCCAAGTAATGAA 5 0.125 No Hit GTTTTGATGTTCATCTCCAAACAATATCATCGAACGGCCAAATGTAAAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.11249999999999999 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.2375 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.425 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.8375 0.0 0.0 0.0 0.0 90-91 0.975 0.0 0.0 0.0 0.0 92-93 1.0499999999999998 0.0 0.0 0.0 0.0 94-95 1.2 0.0 0.0 0.0 0.0 96-97 1.4125 0.0 0.0 0.0 0.0 98-99 1.6625 0.0 0.0 0.0 0.0 100-101 2.0625 0.0 0.0 0.0 0.0 102-103 2.275 0.0 0.0 0.0 0.0 104-105 2.6 0.0 0.0 0.0 0.0 106-107 2.825 0.0 0.0 0.0 0.0 108-109 3.0999999999999996 0.0 0.0 0.0 0.0 110-111 3.3125 0.0 0.0 0.0 0.0 112-113 3.525 0.0 0.0 0.0 0.0 114-115 4.1625 0.0 0.0 0.0 0.0 116-117 4.574999999999999 0.0 0.0 0.0 0.0 118-119 5.0125 0.0 0.0 0.0 0.0 120-121 5.625 0.0 0.0 0.0 0.0 122-123 6.1125 0.0 0.0 0.0 0.0 124-125 6.525 0.0 0.0 0.0 0.0 126-127 7.0375 0.0 0.0 0.0 0.0 128-129 7.5875 0.0 0.0 0.0 0.0 130-131 8.1375 0.0 0.0 0.0 0.0 132-133 8.725000000000001 0.0 0.0 0.0 0.0 134-135 9.175 0.0 0.0 0.0 0.0 136-137 9.8 0.0 0.0 0.0 0.0 138-139 10.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTAGA 10 0.006830828 145.0 3 CTGCGAT 10 0.006830828 145.0 1 >>END_MODULE SRR13695407 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695407_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.14 37.0 37.0 37.0 37.0 37.0 2 36.007 37.0 37.0 37.0 37.0 37.0 3 35.9415 37.0 37.0 37.0 37.0 37.0 4 36.0795 37.0 37.0 37.0 37.0 37.0 5 36.192 37.0 37.0 37.0 37.0 37.0 6 36.1235 37.0 37.0 37.0 37.0 37.0 7 36.1465 37.0 37.0 37.0 37.0 37.0 8 36.2435 37.0 37.0 37.0 37.0 37.0 9 36.199 37.0 37.0 37.0 37.0 37.0 10-14 36.179700000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.2185 37.0 37.0 37.0 37.0 37.0 20-24 36.176550000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.20315 37.0 37.0 37.0 37.0 37.0 30-34 36.1309 37.0 37.0 37.0 37.0 37.0 35-39 36.09955 37.0 37.0 37.0 37.0 37.0 40-44 36.1206 37.0 37.0 37.0 37.0 37.0 45-49 36.06955000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.0218 37.0 37.0 37.0 37.0 37.0 55-59 36.00175 37.0 37.0 37.0 37.0 37.0 60-64 36.012750000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.97115 37.0 37.0 37.0 37.0 37.0 70-74 35.97405 37.0 37.0 37.0 37.0 37.0 75-79 35.94715 37.0 37.0 37.0 37.0 37.0 80-84 35.915499999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.8451 37.0 37.0 37.0 37.0 37.0 90-94 35.87065 37.0 37.0 37.0 37.0 37.0 95-99 35.8295 37.0 37.0 37.0 37.0 37.0 100-104 35.71945 37.0 37.0 37.0 37.0 37.0 105-109 35.7498 37.0 37.0 37.0 37.0 37.0 110-114 35.68265 37.0 37.0 37.0 37.0 37.0 115-119 35.62365 37.0 37.0 37.0 37.0 37.0 120-124 35.57105 37.0 37.0 37.0 37.0 37.0 125-129 35.55625 37.0 37.0 37.0 37.0 37.0 130-134 35.32185 37.0 37.0 37.0 32.2 37.0 135-139 35.2553 37.0 37.0 37.0 34.6 37.0 140-144 35.05035 37.0 37.0 37.0 27.4 37.0 145-149 34.9363 37.0 37.0 37.0 25.0 37.0 150-151 34.727000000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 2.0 15 0.0 16 0.0 17 0.0 18 1.0 19 2.0 20 1.0 21 1.0 22 4.0 23 4.0 24 7.0 25 7.0 26 9.0 27 11.0 28 22.0 29 19.0 30 25.0 31 44.0 32 84.0 33 109.0 34 211.0 35 586.0 36 2661.0 37 186.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.27008032128514 21.335341365461847 13.805220883534137 28.589357429718877 2 27.725 26.05 29.975 16.25 3 20.0 27.900000000000002 33.6 18.5 4 23.7 30.575000000000003 25.124999999999996 20.599999999999998 5 25.95 34.925 22.55 16.575 6 20.925 38.35 22.8 17.925 7 22.225 21.15 38.35 18.275 8 23.599999999999998 26.224999999999998 27.650000000000002 22.525000000000002 9 24.6 24.65 28.775000000000002 21.975 10-14 23.635 29.185 26.855 20.325 15-19 23.45 27.755000000000003 27.935 20.86 20-24 23.410534740633285 28.09264168875994 28.302736231304088 20.194087339302687 25-29 23.27745809357018 27.73580185138854 28.04103077307981 20.945709281961474 30-34 23.08385031018611 27.951771062637583 28.89733840304182 20.06704022413448 35-39 23.562959627795287 27.36004802641453 27.87533143228776 21.201660913502426 40-44 22.648589153492097 28.44206523914349 27.931759055433258 20.97758655193116 45-49 23.362849567261996 28.075441492821053 27.700235129321126 20.861473810595825 50-54 23.881940970485243 28.194097048524263 27.1935967983992 20.730365182591296 55-59 22.697022767075307 27.410557918438826 28.641481110833123 21.25093820365274 60-64 23.0076542098154 28.02541397768773 27.720246135374456 21.246685677122418 65-69 23.485871467866968 28.052013003250813 27.916979244811202 20.545136284071017 70-74 23.742807105328996 27.280460345258945 27.990993244933698 20.98573930447836 75-79 23.014959723820482 27.16765897833592 27.933156551758643 21.884224746084953 80-84 23.741870935467734 27.973986993496748 27.238619309654826 21.045522761380692 85-89 23.806664665265686 28.229760832582805 27.073951766236366 20.88962273591514 90-94 24.118265045775175 27.980389214067735 27.45510030516784 20.446245434989244 95-99 23.516758379189596 28.049024512256125 27.693846923461727 20.740370185092548 100-104 24.34325744308231 28.361270953214913 27.295471603702776 20.0 105-109 24.241969378564995 27.329130391273893 28.74011808265786 19.688782147503254 110-114 24.693581469808397 28.235529541247683 26.814748111461306 20.256140877482615 115-119 24.761094711562514 28.033221594036124 27.34277280232151 19.862910892079853 120-124 25.14886164623468 27.975981986489867 27.025268951713787 19.84988741556167 125-129 25.56661830189623 27.97818582078351 26.44719067393806 20.008005203382197 130-134 25.72944297082228 27.34097392522897 27.61123066913568 19.31835243481307 135-139 26.53622898318655 26.906525220176142 26.89151321056846 19.665732586068856 140-144 26.71969583270799 27.440092050627847 26.939816899294613 18.900395217369553 145-149 27.68714971977582 27.141713370696557 26.20096076861489 18.97017614091273 150-151 28.16020025031289 26.44555694618273 27.23404255319149 18.16020025031289 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 0.5 20 0.5 21 0.5 22 0.5 23 3.5 24 4.5 25 1.5 26 0.0 27 3.0 28 9.5 29 12.5 30 14.0 31 13.5 32 22.0 33 38.5 34 50.5 35 66.5 36 97.5 37 112.5 38 124.5 39 165.5 40 174.0 41 196.0 42 264.0 43 275.0 44 274.0 45 291.5 46 288.0 47 250.5 48 214.5 49 196.5 50 156.0 51 131.5 52 117.5 53 93.0 54 80.5 55 65.5 56 56.0 57 42.5 58 20.5 59 15.0 60 12.0 61 11.5 62 9.5 63 5.5 64 2.5 65 1.5 66 0.5 67 0.0 68 0.5 69 1.5 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.045 25-29 0.075 30-34 0.06 35-39 0.055 40-44 0.06 45-49 0.055 50-54 0.05 55-59 0.075 60-64 0.055 65-69 0.025 70-74 0.075 75-79 0.065 80-84 0.05 85-89 0.06999999999999999 90-94 0.055 95-99 0.05 100-104 0.075 105-109 0.06999999999999999 110-114 0.055 115-119 0.065 120-124 0.075 125-129 0.065 130-134 0.095 135-139 0.08 140-144 0.055 145-149 0.08 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.95 #Duplication Level Percentage of deduplicated Percentage of total 1 71.33922001471672 48.475 2 17.91758646063282 24.349999999999998 3 6.548933038999265 13.350000000000001 4 2.35467255334805 6.4 5 0.9565857247976454 3.25 6 0.515084621044886 2.1 7 0.11037527593818984 0.525 8 0.14716703458425312 0.8 9 0.07358351729212656 0.44999999999999996 >10 0.03679175864606328 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 12 0.3 No Hit GAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCA 9 0.22499999999999998 No Hit CCAGAGGAAAGGGTATGGTGGACTCTCTTTTCCAAGCCCCCCAGGGAACT 9 0.22499999999999998 No Hit TGTCAAGAGAAATGACAAGAAGGTACATGATGGACATGATAACTCATGCA 8 0.2 No Hit AAAAAAAGAAGAAATCTCCCTATCATTTTTCTTTCTTTTCTTCCCCATGT 8 0.2 No Hit ATCTGCTCCAGCGATTTCTGATTTAAATTCTGGGCGGGGTTTAATTGAAT 8 0.2 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 8 0.2 No Hit TATGATGGCTGCAACTGATCTCTTAGCTGCTGGTTTTACTGCAGTGACTG 7 0.17500000000000002 No Hit GATGTGTCAATCCCTGTTGTACTGATTCCAAAGTCAGGGGGTCAATCTCT 7 0.17500000000000002 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 7 0.17500000000000002 No Hit GTCTCTTGTCATTCGAATTCTGATCCTTCAACACTACCACCATCGTCTGA 6 0.15 No Hit CTGTAGACCGAAATCACTCAACACCTTCCAATCTCCAATCCTTCATGGCA 6 0.15 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit GATGTTGGTTACCCAGGTGGGCTATGGTTTGACCCACTTGGATGGGGCAG 6 0.15 No Hit GGGACTGCACATTTCCTTGCATGCAGGGAACTGGATTACCAACAAAGCCT 6 0.15 No Hit TCCTCATCCACAAGAGATAATTGAGTTTCCATCTGTCATCGTGAGCAGTG 6 0.15 No Hit GTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGA 6 0.15 No Hit GATCCATATGTTGTTACTTCCATGATTGCAGAAACCACCATCCTGTGGAA 6 0.15 No Hit CCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGGAAACTGTGGGTGTGG 6 0.15 No Hit GAACTACTACGATTTTTCAAAAGGAGGTACTCATCCGAGTTTGTTGGTGG 6 0.15 No Hit AAAGATTGCTTGTTTCAAAGTGAACACCGATGATTGCCCAAACATTGCAT 6 0.15 No Hit TTTTCTTTCCGATTTAACACTAGATTGTAAGAAAGGAAGGAAAAAAACCC 6 0.15 No Hit AACAAACTGACATTCTTATTTAATATTCCATTATTTATTATTACTGATCA 6 0.15 No Hit CGGCGATTGAACTAGTAAGGGTCCATCTAATTATTCTCATCCTCTGGGAA 6 0.15 No Hit TAAGGATGGTTCTGATTCTCGAGCTGTGAATAGTGTTATTAGGAGGGCTG 5 0.125 No Hit CTTTTCTTTCCGATTTAACACTAGATTGTAAGAAAGGAAGGAAAAAAACC 5 0.125 No Hit CTTGCCCATCATTCCTGTTGTTGGAGATTACTGGGTTCTGTCTCTTGATG 5 0.125 No Hit CGCAGCTTTCGCTTGAATCAAAAGAGCTCTCTTTTAGCAGAAAAACAATG 5 0.125 No Hit TGTTTTTCCTTGAAAAGGGCACCCCAGATGAACAAAGAATCAAGAGGACA 5 0.125 No Hit CCAACTTCCGTGAGGTTGCAATTAAAGGATTTTGGGAAATGTATGATCCT 5 0.125 No Hit ATCTAAGAGAAAGGCCAGAATTCTCGAAGAGATGGACACTGCAGAGAAAG 5 0.125 No Hit GTTGATGATGGAAGTGCTGATGCGACAAAACGAGTAGCTTTTGACTTCGT 5 0.125 No Hit GGATGGATGATCAAGGTGAAGCCAAGCAACCCATCAGAATTACAGTCCCT 5 0.125 No Hit TGGGAGAAGATTGTTGGAGACAAAGGAAAGGCTATTGGAATTGACCGGTT 5 0.125 No Hit ATTCAATAGAATGCACTCTTACTTTTCAAAGTTGGCTTATTCAATAGTGC 5 0.125 No Hit GCTCAGATTAACACCAAAATAAATCCAGAGTTGCTGAGATCCTTGGGAGA 5 0.125 No Hit GAACCATGCCATTATTTTCACTCGCGGGGAAGCCTTGCAAACAATAGACA 5 0.125 No Hit GCACTGAGAATCTCTGTTGCCTCCTCCACAGCTCACCTCTCTCCTTCCTA 5 0.125 No Hit ATTTTCGCCACTGGATCTGGCATCAGTCCAATTCGATCTCTTATAGAGTC 5 0.125 No Hit TGGTGAGAGTGTATTGCCACTTTATAGCTTCGTGGATTATGCTGCTGATA 5 0.125 No Hit AACCCTAACAACCACCGCCCAAACCGGGCACAACCCAATCGTCGTAAGCG 5 0.125 No Hit GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 5 0.125 No Hit CATTTTGGCCATATACACCTTCCATCCAATTGTTGTATGGACTAAGAGAA 5 0.125 No Hit AAGGAAGGGATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGGAAGCA 5 0.125 No Hit AGAGGTTCTTTGTTCAATGCAAGCAGCACGCTGATTCACACAAGAGTGAG 5 0.125 No Hit GGTTGATGGAATGCTCAAAGACGGTTTGTGGGATGTTTATAATGATTATG 5 0.125 No Hit CCAAGTGGCTCTCTCTCACAAGGAGTTGAACAAGTCTACCATCAAACGTG 5 0.125 No Hit CCCCAGCCGTCCCTGGAATCATGTTTTTGTCTGGTGGGCAATCTGAGGTC 5 0.125 No Hit GCTAGCTAGAAGTGACTCTACCCTTTGCATTACTTTTTCAATCAATCACT 5 0.125 No Hit GATCTCTCAAGCAATGGTTTTGAAGGAGGAATTCCAGAGGCCCTCGGAGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.11249999999999999 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.2375 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.4 0.0 0.0 0.0 0.0 82-83 0.425 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.8375 0.0 0.0 0.0 0.0 90-91 1.0 0.0 0.0 0.0 0.0 92-93 1.0750000000000002 0.0 0.0 0.0 0.0 94-95 1.25 0.0 0.0 0.0 0.0 96-97 1.4625 0.0 0.0 0.0 0.0 98-99 1.7125 0.0 0.0 0.0 0.0 100-101 2.1125 0.0 0.0 0.0 0.0 102-103 2.375 0.0 0.0 0.0 0.0 104-105 2.7 0.0 0.0 0.0 0.0 106-107 2.925 0.0 0.0 0.0 0.0 108-109 3.2 0.0 0.0 0.0 0.0 110-111 3.4124999999999996 0.0 0.0 0.0 0.0 112-113 3.625 0.0 0.0 0.0 0.0 114-115 4.2625 0.0 0.0 0.0 0.0 116-117 4.675000000000001 0.0 0.0 0.0 0.0 118-119 5.1125 0.0 0.0 0.0 0.0 120-121 5.75 0.0 0.0 0.0 0.0 122-123 6.25 0.0 0.0 0.0 0.0 124-125 6.725 0.0 0.0 0.0 0.0 126-127 7.2875 0.0 0.0 0.0 0.0 128-129 7.862500000000001 0.0 0.0 0.0 0.0 130-131 8.4375 0.0 0.0 0.0 0.0 132-133 9.0625 0.0 0.0 0.0 0.0 134-135 9.5 0.0 0.0 0.0 0.0 136-137 10.125 0.0 0.0 0.0 0.0 138-139 10.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGGGG 110 8.488292E-6 13.212889 140-144 >>END_MODULE Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912587 spots for SRR13695407.sra Written 912587 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra Read 912577 spots for SRR13695407.sra Written 912577 spots for SRR13695407.sra SRR ids: ['SRR13695407.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_h66a119x SRR13695407.sra spots: 18251550 blocks: [[1, 912577], [912578, 1825154], [1825155, 2737731], [2737732, 3650308], [3650309, 4562885], [4562886, 5475462], [5475463, 6388039], [6388040, 7300616], [7300617, 8213193], [8213194, 9125770], [9125771, 10038347], [10038348, 10950924], [10950925, 11863501], [11863502, 12776078], [12776079, 13688655], [13688656, 14601232], [14601233, 15513809], [15513810, 16426386], [16426387, 17338963], [17338964, 18251550]] SRR13695407 file size 6180974 SRR13695407 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695407 SRR13695407_1.fastq SRR13695407_2.fastq Input file: SRR13695407_1.fastq Paired file: SRR13695407_2.fastq trimmed: SRR13695407-trimmed-pair1.fastq, SRR13695407-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:59:14 2025 >> started Wed Feb 12 00:59:35 2025 >> done (21.549s) 18251550 read pairs processed; of these: 154 ( 0.00%) short read pairs filtered out after trimming by size control 9857 ( 0.05%) empty read pairs filtered out after trimming by size control 18241539 (99.95%) read pairs available; of these: 2768790 (15.18%) trimmed read pairs available after processing 15472749 (84.82%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 2 0.00% 20 0 0.00% 21 2 0.00% 22 1 0.00% 23 4 0.00% 24 1 0.00% 25 3 0.00% 26 1 0.00% 27 4 0.00% 28 6 0.00% 29 3 0.00% 30 2 0.00% 31 3 0.00% 32 4 0.00% 33 5 0.00% 34 6 0.00% 35 6 0.00% 36 17 0.00% 37 4 0.00% 38 11 0.00% 39 21 0.00% 40 30 0.00% 41 33 0.00% 42 21 0.00% 43 36 0.00% 44 44 0.00% 45 43 0.00% 46 57 0.00% 47 62 0.00% 48 78 0.00% 49 112 0.00% 50 131 0.00% 51 162 0.00% 52 147 0.00% 53 177 0.00% 54 208 0.00% 55 190 0.00% 56 252 0.00% 57 259 0.00% 58 281 0.00% 59 430 0.00% 60 480 0.00% 61 564 0.00% 62 605 0.00% 63 665 0.00% 64 739 0.00% 65 855 0.00% 66 925 0.01% 67 1058 0.01% 68 1248 0.01% 69 1343 0.01% 70 1662 0.01% 71 1883 0.01% 72 2088 0.01% 73 2477 0.01% 74 2770 0.02% 75 3207 0.02% 76 3516 0.02% 77 3682 0.02% 78 4073 0.02% 79 4671 0.03% 80 5195 0.03% 81 5642 0.03% 82 6272 0.03% 83 6882 0.04% 84 8089 0.04% 85 8678 0.05% 86 9410 0.05% 87 9991 0.05% 88 10834 0.06% 89 11577 0.06% 90 12543 0.07% 91 13173 0.07% 92 13863 0.08% 93 15486 0.08% 94 16286 0.09% 95 17074 0.09% 96 17810 0.10% 97 19756 0.11% 98 20403 0.11% 99 20924 0.11% 100 21895 0.12% 101 22398 0.12% 102 23532 0.13% 103 24388 0.13% 104 25799 0.14% 105 27210 0.15% 106 28536 0.16% 107 29717 0.16% 108 30732 0.17% 109 31632 0.17% 110 32105 0.18% 111 33562 0.18% 112 33986 0.19% 113 35510 0.19% 114 37570 0.21% 115 38084 0.21% 116 39544 0.22% 117 41130 0.23% 118 42383 0.23% 119 42573 0.23% 120 44250 0.24% 121 46079 0.25% 122 45774 0.25% 123 46947 0.26% 124 48154 0.26% 125 48716 0.27% 126 50963 0.28% 127 52011 0.29% 128 52897 0.29% 129 53731 0.29% 130 54895 0.30% 131 55362 0.30% 132 55567 0.30% 133 57408 0.31% 134 57982 0.32% 135 58831 0.32% 136 59170 0.32% 137 61383 0.34% 138 62230 0.34% 139 64480 0.35% 140 64636 0.35% 141 65595 0.36% 142 66199 0.36% 143 66161 0.36% 144 68051 0.37% 145 68008 0.37% 146 69389 0.38% 147 70344 0.39% 148 72805 0.40% 149 72976 0.40% 150 74247 0.41% 151 15472749 84.82% 18241539 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=25 prefix-density=0.35 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=90.57 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=7.9 sequence=AAACAGAATATTTACTTTTAGCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATTGAGAACATAGCCAACCT criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.63 fanout-score-rank=21 prefix-density=0.49 prefix-fanout=2.5 sequence=TGCAAGTGCGGCAG criterion=fanout-score sequence-density=0.24 sequence-density-rank=9 fanout-score=14.45 fanout-score-rank=1 prefix-density=0.50 prefix-fanout=6.9 sequence=AAGAAAGCTTACCCTAAC SRR13695407 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:00:17 Started mapping on | Feb 12 01:00:18 Finished on | Feb 12 01:02:49 Mapping speed, Million of reads per hour | 434.90 Number of input reads | 18241539 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 16842761 Uniquely mapped reads % | 92.33% Average mapped length | 292.78 Number of splices: Total | 16496582 Number of splices: Annotated (sjdb) | 16119050 Number of splices: GT/AG | 16159476 Number of splices: GC/AG | 249270 Number of splices: AT/AC | 10213 Number of splices: Non-canonical | 77623 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.87 Insertion rate per base | 0.02% Insertion average length | 2.30 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 444757 % of reads mapped to multiple loci | 2.44% Number of reads mapped to too many loci | 35942 % of reads mapped to too many loci | 0.20% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.91% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 954276 954276 954276 N_multimapping 444757 444757 444757 N_noFeature 574096 16360904 801583 N_ambiguous 366707 1743 111091 UnstrandedReadsAssigned:15901958 PositiveStrandReadsAssigned:480114 NegativeStrandReadsAssigned:15930087 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695407 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695407-trimmed-pair1.fastq SRR13695407-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,241,539 reads, 15,915,312 reads pseudoaligned [quant] estimated average fragment length: 230.252 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,060 rounds 52401 SRR13695407.ke.tsv 34699 SRR13695407.se.tsv 87100 total ==> SRR13695407.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1788.75 987 25.0816 Potri.005G024800.1.v4.1 1035 805.748 502 28.3199 Potri.004G059700.1.v4.1 961 731.817 0 0 Potri.007G009000.2.v4.1 1416 1186.75 0 0 Potri.003G141000.2.v4.1 2943 2713.75 1088.5 18.2325 Potri.016G087400.1.v4.1 270 90.2305 1525.15 768.329 Potri.015G069301.1.v4.1 564 340.444 0 0 Potri.010G195200.1.v4.1 1773 1543.75 528 15.5469 Potri.012G127500.1.v4.1 977 747.791 52 3.16089 ==> SRR13695407.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 103 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 359 Potri.001G212900.v4.1 12 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 44 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695407 completed mapping pipeline successfully