Starting /dee2/code/volunteer_pipeline.sh SRR13695409 current disk space = 3051181506560 free memory = 1083760628 SRR13695409 SRAfilesize d4ed4006203879e4faf216255bfc9489 SRR13695409.sra SRR13695409.sra file validated SRR13695409 is paired end SRR13695409 is conventional basespace SRR13695409 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695409_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.499 37.0 37.0 37.0 37.0 37.0 2 36.3755 37.0 37.0 37.0 37.0 37.0 3 36.5405 37.0 37.0 37.0 37.0 37.0 4 36.554 37.0 37.0 37.0 37.0 37.0 5 36.5995 37.0 37.0 37.0 37.0 37.0 6 36.5325 37.0 37.0 37.0 37.0 37.0 7 36.37 37.0 37.0 37.0 37.0 37.0 8 36.479 37.0 37.0 37.0 37.0 37.0 9 36.4325 37.0 37.0 37.0 37.0 37.0 10-14 36.497 37.0 37.0 37.0 37.0 37.0 15-19 36.5027 37.0 37.0 37.0 37.0 37.0 20-24 36.4671 37.0 37.0 37.0 37.0 37.0 25-29 36.4317 37.0 37.0 37.0 37.0 37.0 30-34 36.4132 37.0 37.0 37.0 37.0 37.0 35-39 36.40050000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.3719 37.0 37.0 37.0 37.0 37.0 45-49 36.3425 37.0 37.0 37.0 37.0 37.0 50-54 36.324799999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3524 37.0 37.0 37.0 37.0 37.0 60-64 36.325199999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.2377 37.0 37.0 37.0 37.0 37.0 70-74 36.2636 37.0 37.0 37.0 37.0 37.0 75-79 36.2567 37.0 37.0 37.0 37.0 37.0 80-84 36.195499999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.2053 37.0 37.0 37.0 37.0 37.0 90-94 36.151300000000006 37.0 37.0 37.0 37.0 37.0 95-99 36.1888 37.0 37.0 37.0 37.0 37.0 100-104 36.081 37.0 37.0 37.0 37.0 37.0 105-109 36.158 37.0 37.0 37.0 37.0 37.0 110-114 36.1559 37.0 37.0 37.0 37.0 37.0 115-119 36.0913 37.0 37.0 37.0 37.0 37.0 120-124 36.0025 37.0 37.0 37.0 37.0 37.0 125-129 35.994 37.0 37.0 37.0 37.0 37.0 130-134 35.9827 37.0 37.0 37.0 37.0 37.0 135-139 35.9247 37.0 37.0 37.0 37.0 37.0 140-144 35.8458 37.0 37.0 37.0 37.0 37.0 145-149 35.6623 37.0 37.0 37.0 37.0 37.0 150-151 35.548 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 2.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 1.0 24 1.0 25 2.0 26 4.0 27 10.0 28 17.0 29 29.0 30 35.0 31 40.0 32 38.0 33 64.0 34 104.0 35 300.0 36 2965.0 37 386.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.05 11.375 14.875 46.7 2 19.468138484696436 14.626191670847968 34.44555945810336 31.46011038635223 3 19.85 17.325 23.549999999999997 39.275 4 23.125 25.0 21.675 30.2 5 24.275 30.8 24.325 20.599999999999998 6 22.275 36.525 21.775 19.425 7 15.425 27.150000000000002 39.975 17.45 8 17.45 27.175 33.050000000000004 22.325 9 18.65 25.05 34.775 21.525 10-14 18.995 30.72 26.995 23.29 15-19 19.975 27.935 28.335 23.755000000000003 20-24 19.31 29.175 27.965 23.549999999999997 25-29 20.05 28.244999999999997 27.455000000000002 24.25 30-34 19.78 29.535 27.325 23.36 35-39 20.57 28.105000000000004 27.425 23.9 40-44 19.865 28.625 27.735 23.775 45-49 20.055 28.384999999999998 28.255000000000003 23.305 50-54 19.925 28.255000000000003 27.51 24.310000000000002 55-59 19.495 28.895 28.13 23.48 60-64 19.59 29.425 27.060000000000002 23.925 65-69 20.69 28.910000000000004 27.04 23.36 70-74 19.97 28.925 27.52 23.585 75-79 19.91 28.315 27.985 23.79 80-84 20.02 28.410000000000004 27.61 23.96 85-89 20.135 28.965000000000003 26.895000000000003 24.005000000000003 90-94 20.32 28.68 27.08 23.919999999999998 95-99 21.38 27.800000000000004 26.905 23.915 100-104 20.49 28.12 27.48 23.91 105-109 21.115000000000002 28.04 27.295 23.549999999999997 110-114 20.46 28.315 26.85 24.375 115-119 21.02 28.235 26.77 23.974999999999998 120-124 20.625 28.475 26.52 24.38 125-129 21.66 28.78 25.840000000000003 23.72 130-134 21.015 28.794999999999998 26.340000000000003 23.849999999999998 135-139 21.16 29.07 25.4 24.37 140-144 21.42 28.82 25.215 24.545 145-149 21.01 28.799999999999997 25.81 24.38 150-151 23.0875 28.712500000000002 24.2875 23.9125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.5 23 2.5 24 3.5 25 3.5 26 5.5 27 6.0 28 7.0 29 8.5 30 20.0 31 29.0 32 34.5 33 48.0 34 61.5 35 68.5 36 93.0 37 113.0 38 126.0 39 148.0 40 184.5 41 217.5 42 221.5 43 243.0 44 263.0 45 275.0 46 282.0 47 247.5 48 220.5 49 225.5 50 184.5 51 139.5 52 113.0 53 84.5 54 74.5 55 58.0 56 45.0 57 42.0 58 29.5 59 18.0 60 21.0 61 15.5 62 4.5 63 3.0 64 2.5 65 1.5 66 0.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.35000000000000003 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.30000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 70.42459736456809 48.1 2 19.070278184480234 26.05 3 6.808199121522694 13.950000000000001 4 2.0863836017569546 5.7 5 0.9150805270863837 3.125 6 0.43923865300146414 1.7999999999999998 7 0.21961932650073207 1.05 8 0.0 0.0 9 0.036603221083455345 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCC 9 0.22499999999999998 No Hit CTCCATTTGACAACCGTCCCCCAATATAGCATATGTGTAGTGGTCAACAA 7 0.17500000000000002 No Hit ACCAATTCTCGAGCTGCCATGGATAAAGGAGAGAAGGCAATGGTAGCTAT 7 0.17500000000000002 No Hit TCGCATGTACTGTACCGCCATCTTAACAGTAAATTTTGGAAGCCTGATTT 7 0.17500000000000002 No Hit ATAGTAGTTAGCCCTGTATAGATCGTATGCATGTGGGAGCAAGCGCACAA 7 0.17500000000000002 No Hit GGACTTTTATTTATCAGTCCAGGAGTTTCACTAAGCTTTGTATTAACCTC 7 0.17500000000000002 No Hit CTTGTGCCTGCTCTTTGGATCAACTGCATCTTTCTCGCTGTGCGGTAAAA 7 0.17500000000000002 No Hit GTCGAATCCAATGATACGGATAAAGGAGTTAGGGTAAGCTTTCTTCGCCT 6 0.15 No Hit CTCCGCCACCTGAACTAGAAGAATCACAACCTGAACCACCATTAATGCTT 6 0.15 No Hit GGGCTGAAAAGGATTAAAATGTGCCTGGTTTTGCTGATTCAAAGCAGCAT 6 0.15 No Hit CCTTGTTATTTCAAGCTAAAGGTTCTCCTTTTGAATCTCAGTTGCTAGAT 6 0.15 No Hit CATAAAAACAACCTAAATGCAATCTCATGTGCATTTTTCCAGGTATGCAT 6 0.15 No Hit CCCTCTCTTGGTGAATGGAGCTGCAAATGTCTCAAAGAATTTGCTCTGGA 6 0.15 No Hit GCTGGCAGAACACTTTCCTTGTACGCAACTGATTGCTCATCAAAAAGCTC 6 0.15 No Hit GGCCAGTGTAGATTCAACCTGTTTCTTGGTCAAAGGCAAAGTAGCAACTT 6 0.15 No Hit GAACTGTGAATGCTCTTGCAAAATTTGCTCCACTAGTGGCCAATCTCTTC 6 0.15 No Hit CCAGGTCACCAACCATAACCAAAACTGAGAAGTGAGCGGAGGAGGGGTGG 6 0.15 No Hit CTTCCTGTTGCTGCAGCTGCTGCTCCTGTTTACGAGCATCTGCCATCTGT 6 0.15 No Hit TTGAAATAATAATAACGATGGACGGTGACCTTTTTTCCGAGATGATATTG 6 0.15 No Hit GTGGGGTTGTCGAAGGCTGTTTGGCCATGGCGGAACCAGTTAGGAATGAG 5 0.125 No Hit GCTGTATGAGTTGGAAGACCCATGAAAGATGAGAGAGATAGAGAATCAGT 5 0.125 No Hit CGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAG 5 0.125 No Hit CGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCA 5 0.125 No Hit CCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATT 5 0.125 No Hit AATGAACTTCCCAGATGTGGTTGAGCAGGTTGATGAAGGGATATTTTGAT 5 0.125 No Hit GCTAAATCCAGGTGGAGGACCAAGGTGATGAACAGGACAGCTTACTGGAT 5 0.125 No Hit CTGTAATATCTGCAACCAACTCTGCTTCAATTTTTTGCAACCAACTTACT 5 0.125 No Hit GTTAGCTGTTGCCACGGTATTGGGGTCGAAACCACCCTAAGATGCGGTTG 5 0.125 No Hit CTATAATGTTATAAACATGACAAGAACAACCACTTACAACAATCTTGACT 5 0.125 No Hit CCACCCTCAGATGCAAAACTGCATACTCTACTGCTGCCCCAACTCCAGCG 5 0.125 No Hit CTGAAGCTAAAATATTAAAAACAAAACCCGACCCGAATACCCACTCAATC 5 0.125 No Hit CGGGAAAGCTGAGGTAGACTTGAGGCCGTTGAATGGTGCAACCATGTTGG 5 0.125 No Hit GACATCTTTAAATAAAAAGGCATGCTCTCAACTTCCCAGAGTAAGTTATT 5 0.125 No Hit ACCACCATAGCATCTTGGCCCAAATATCCTGCTAGCCACCGTGCATTCCT 5 0.125 No Hit CATGACTAGATATTGTTGTTGAGCTTCAGTTCTTATCTCTTCTTTCGTCT 5 0.125 No Hit CCTGAAGATGATGTTGGGATCTTGCTCGATAAAATCCCTCCTTCCAAAGG 5 0.125 No Hit CTAGCATTGCCCATCTGCAGTGAACAAGTTCTGCTTGCACATTCCATCTC 5 0.125 No Hit AGCTTATTTTCTACCTCAATTTCACAGAGGACTCTTTGGGTCACTCCATC 5 0.125 No Hit CTGGAAAGGGGTTCAGAGGGGGGTCTATTCCTATCCCCAAACAGGCAAGA 5 0.125 No Hit TGCAGCTCTTTGGGGTCACAGAAAAGTTTAGCAGCATCTTGAACATGTAG 5 0.125 No Hit TGCTCATGTTCTCTAATTTGCATTCTCTGCAAGTGGGCCTGCAAATTCGA 5 0.125 No Hit GGCTAAATCCTCCACTAGTAATTTCTCCAATGTTAGTCCCTTTCTCATCC 5 0.125 No Hit CTTCCCATAGCAGCTGGATAAACAGGAGCAGAAACCCCTGACAAATGTGC 5 0.125 No Hit CTCCCAGGTTCAATAATGAGATTGAGATCTCGTGAGAGCACCAGTTCACG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.25 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.55 0.0 0.0 0.0 0.0 84-85 0.55 0.0 0.0 0.0 0.0 86-87 0.65 0.0 0.0 0.0 0.0 88-89 0.8 0.0 0.0 0.0 0.0 90-91 0.8625 0.0 0.0 0.0 0.0 92-93 0.9874999999999999 0.0 0.0 0.0 0.0 94-95 1.1375000000000002 0.0 0.0 0.0 0.0 96-97 1.2125 0.0 0.0 0.0 0.0 98-99 1.3125 0.0 0.0 0.0 0.0 100-101 1.625 0.0 0.0 0.0 0.0 102-103 1.9 0.0 0.0 0.0 0.0 104-105 2.3375 0.0 0.0 0.0 0.0 106-107 2.625 0.0 0.0 0.0 0.0 108-109 2.925 0.0 0.0 0.0 0.0 110-111 3.15 0.0 0.0 0.0 0.0 112-113 3.4000000000000004 0.0 0.0 0.0 0.0 114-115 3.575 0.0 0.0 0.0 0.0 116-117 3.875 0.0 0.0 0.0 0.0 118-119 4.1625 0.0 0.0 0.0 0.0 120-121 4.7 0.0 0.0 0.0 0.0 122-123 5.3375 0.0 0.0 0.0 0.0 124-125 5.575 0.0 0.0 0.0 0.0 126-127 6.025 0.0 0.0 0.0 0.0 128-129 6.5125 0.0 0.0 0.0 0.0 130-131 7.112500000000001 0.0 0.0 0.0 0.0 132-133 7.725 0.0 0.0 0.0 0.0 134-135 8.649999999999999 0.0 0.0 0.0 0.0 136-137 9.0 0.0 0.0 0.0 0.0 138-139 9.5125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695409 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695409_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1875 37.0 37.0 37.0 37.0 37.0 2 36.258 37.0 37.0 37.0 37.0 37.0 3 36.2095 37.0 37.0 37.0 37.0 37.0 4 36.2755 37.0 37.0 37.0 37.0 37.0 5 36.3565 37.0 37.0 37.0 37.0 37.0 6 36.3 37.0 37.0 37.0 37.0 37.0 7 36.363 37.0 37.0 37.0 37.0 37.0 8 36.375 37.0 37.0 37.0 37.0 37.0 9 36.3345 37.0 37.0 37.0 37.0 37.0 10-14 36.3116 37.0 37.0 37.0 37.0 37.0 15-19 36.329899999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.25625 37.0 37.0 37.0 37.0 37.0 25-29 36.2271 37.0 37.0 37.0 37.0 37.0 30-34 36.1657 37.0 37.0 37.0 37.0 37.0 35-39 36.2148 37.0 37.0 37.0 37.0 37.0 40-44 36.193 37.0 37.0 37.0 37.0 37.0 45-49 36.1268 37.0 37.0 37.0 37.0 37.0 50-54 36.11370000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.0984 37.0 37.0 37.0 37.0 37.0 60-64 36.0727 37.0 37.0 37.0 37.0 37.0 65-69 36.02905 37.0 37.0 37.0 37.0 37.0 70-74 35.9045 37.0 37.0 37.0 37.0 37.0 75-79 36.0441 37.0 37.0 37.0 37.0 37.0 80-84 35.9775 37.0 37.0 37.0 37.0 37.0 85-89 35.910399999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.8709 37.0 37.0 37.0 37.0 37.0 95-99 35.92555 37.0 37.0 37.0 37.0 37.0 100-104 35.850100000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.88289999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.7645 37.0 37.0 37.0 37.0 37.0 115-119 35.757600000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.6699 37.0 37.0 37.0 37.0 37.0 125-129 35.6666 37.0 37.0 37.0 37.0 37.0 130-134 35.51369999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.557 37.0 37.0 37.0 37.0 37.0 140-144 35.424 37.0 37.0 37.0 34.6 37.0 145-149 35.235299999999995 37.0 37.0 37.0 32.2 37.0 150-151 34.95025 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 0.0 16 1.0 17 2.0 18 0.0 19 0.0 20 0.0 21 1.0 22 2.0 23 4.0 24 4.0 25 8.0 26 8.0 27 6.0 28 15.0 29 26.0 30 26.0 31 36.0 32 48.0 33 90.0 34 212.0 35 586.0 36 2705.0 37 216.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.86466165413534 16.716791979949875 21.979949874686717 35.43859649122807 2 28.225 23.775 29.575000000000003 18.425 3 20.9 27.200000000000003 29.65 22.25 4 21.7 31.775 26.325 20.200000000000003 5 26.974999999999998 33.650000000000006 23.925 15.45 6 22.325 36.75 23.325000000000003 17.599999999999998 7 20.3 21.4 38.75 19.55 8 22.6 26.35 26.900000000000002 24.15 9 19.85 24.325 31.85 23.974999999999998 10-14 23.575 28.475 26.015 21.935 15-19 22.634999999999998 28.16 27.96 21.245 20-24 23.11771474310871 27.590174596027815 27.28500675371454 22.00710390714893 25-29 22.80780780780781 27.772772772772775 28.113113113113116 21.306306306306304 30-34 23.598879103282627 27.496997598078465 27.83226581265012 21.07185748598879 35-39 22.723634180508302 27.46147688613168 28.722233340004 21.092655593356014 40-44 23.503803042433947 27.186749399519616 27.65712570056045 21.65232185748599 45-49 22.675873111177825 28.299809866906834 28.01461022715901 21.00970679475633 50-54 23.759255553331997 27.28637182309386 28.457074244546725 20.497298379027416 55-59 22.62762762762763 27.3973973973974 28.66866866866867 21.306306306306304 60-64 23.4740844506704 26.996197718631176 27.9217530518311 21.60796477886732 65-69 23.658280398139347 27.96478767568649 27.76471765117791 20.612214274996248 70-74 23.23823823823824 27.312312312312315 28.373373373373372 21.076076076076074 75-79 23.628903122498 27.146717373899122 28.252602081665334 20.971777421937553 80-84 24.54727363681841 27.458729364682345 27.72886443221611 20.265132566283143 85-89 23.781403262936642 27.734961465318786 27.925132619357424 20.558502652387148 90-94 23.70422253352011 27.476485891534917 27.596557934760856 21.22273364018411 95-99 24.31837510630847 27.50512782030117 27.450097553654512 20.726399519735857 100-104 23.843843843843842 27.962962962962962 27.402402402402405 20.79079079079079 105-109 24.05905905905906 27.36736736736737 27.762762762762762 20.81081081081081 110-114 24.44966980188113 28.24694816890134 27.671602961777065 19.631779067440462 115-119 24.2992992992993 27.767767767767772 26.97197197197197 20.96096096096096 120-124 24.754754754754753 27.74274274274274 27.557557557557555 19.944944944944947 125-129 25.282754479031126 27.039335401861674 27.995195676108498 19.6827144429987 130-134 24.82982982982983 27.21221221221221 27.51751751751752 20.44044044044044 135-139 25.54054054054054 28.468468468468465 26.226226226226224 19.764764764764763 140-144 26.736041624974987 28.036822093255953 25.76045627376426 19.466680008004804 145-149 26.49149149149149 28.203203203203202 25.945945945945947 19.35935935935936 150-151 28.47847847847848 27.565065065065063 25.788288288288285 18.16816816816817 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 1.5 21 1.5 22 0.0 23 0.5 24 1.0 25 0.5 26 2.0 27 2.5 28 3.0 29 8.5 30 16.0 31 21.5 32 27.0 33 36.5 34 50.0 35 61.0 36 73.0 37 92.0 38 121.5 39 145.0 40 163.0 41 219.0 42 228.0 43 229.5 44 281.0 45 304.5 46 297.0 47 279.0 48 259.5 49 244.5 50 191.5 51 128.0 52 119.5 53 100.0 54 68.5 55 54.0 56 48.0 57 32.5 58 20.5 59 18.0 60 14.0 61 9.0 62 5.5 63 6.0 64 2.5 65 1.5 66 3.0 67 2.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.1 30-34 0.08 35-39 0.06 40-44 0.08 45-49 0.06999999999999999 50-54 0.06 55-59 0.1 60-64 0.06 65-69 0.034999999999999996 70-74 0.1 75-79 0.08 80-84 0.05 85-89 0.09 90-94 0.06 95-99 0.055 100-104 0.1 105-109 0.1 110-114 0.06 115-119 0.1 120-124 0.1 125-129 0.09 130-134 0.1 135-139 0.1 140-144 0.06 145-149 0.1 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.89999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 71.55297532656023 49.3 2 18.069666182873732 24.9 3 6.676342525399129 13.8 4 1.995645863570392 5.5 5 1.0885341074020318 3.75 6 0.43541364296081275 1.7999999999999998 7 0.10885341074020319 0.525 8 0.036284470246734396 0.2 9 0.036284470246734396 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACCAGTCCCCAGGGTACTATGATGGACGCTACTGGACTATGTGGAAACT 9 0.22499999999999998 No Hit GATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTG 8 0.2 No Hit TGTTTTTGCTCTTGATTTTTCGGGTTCTGGCTTATCTGATGGTGATTATG 7 0.17500000000000002 No Hit GCTGTATGTTACAGTATGCTCTGCTTCACCTTGCTGGTTATGATAGTGTC 7 0.17500000000000002 No Hit GCATGGAGTACACTTACAGCAGCACTTATCAGCGTTCCCTTTGGGTGGAT 7 0.17500000000000002 No Hit TGTACACAAGTCTCTCCCACCTCCTGTGCATTCTACTTTTCCACCAGAAA 6 0.15 No Hit TGAAAGCTTTAGTGATAGCTATTCTTATAGCTACCATTGCCTTCTCTCCT 6 0.15 No Hit TGCAAGGCGTTTCAATTTAGACGATATTTCTGGAATTGATCTCAAGTATT 6 0.15 No Hit TCTGAGATCATTAATGAAAGTCTGATTGAATCTGTGGATTCCTTGAATCC 6 0.15 No Hit CAGCCGGCAACTTTGCTAAGGGAGATTACACTGCTGCGGCAGTAAAAATT 6 0.15 No Hit GGTTGAAGTATGCATCTTCACATGAGTGGGTGAAGCATGAAGGGCCGGTG 6 0.15 No Hit TTTTGGTACAGTCAATCCTTCCATAGCAACTTAACTACCAAGCAAATATG 6 0.15 No Hit AGGAAGGATTTTGAATTTGTGAAAACCAGTCTTGCTGCAAAAGGCTTTGA 6 0.15 No Hit TACTGGCCAACTTGTCGAGAGATCTTTCTCCCAACAATATTGGAAAATAT 6 0.15 No Hit GTGGGCTTTCAGCTGTACACGTGCCGTATGCTTATGGTTTTGCCATTATT 6 0.15 No Hit TGGCAAATAGGTTTTGGTTCAGGATTTAAGTGCAACAGTGCCGTGTGGCG 6 0.15 No Hit TGGAATTTGAAGCTCATCTGAGTTCATACGATCATAATCATAGAAAGCGA 6 0.15 No Hit CTTAATTCCTCTTCATCTATATATACCTCTAGATTCTCTGTTAAAAGGTT 5 0.125 No Hit AGATTTCCTTGTACCATCCTACCGGGGCTCTTCTTTCCTGGACCCAAAGG 5 0.125 No Hit CGGTGAGGTAACTGACCCAATCTACCCAGGTGGAAGCTTCGACCCACTGG 5 0.125 No Hit GCACATAACACCAGTAGAGGCAGGACTGAATTGGGCGATAGGGAAGAGAA 5 0.125 No Hit ATCCGAGCCCAGGGATTTGAAGTTGATTACTTAAACATTGGAGGTGGTTT 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit CTTTGGAATTAGCAGCAAATATGCTATATTTGCCGACATACAGATTGGGA 5 0.125 No Hit ATTTAGACAAGCTGTAGAGTACAGGAAAGGAGGGAACATAACAGACAGAG 5 0.125 No Hit GCATAATTCCTAGTCTCTACCTCCTCCTTCTCAGTTTATACATCTCTAAA 5 0.125 No Hit GGAGTTAAATAGTAAGAAAAAGGCACACTTGCATGTGCAAAATGGACACG 5 0.125 No Hit CAAGGACACGAGGAGGTAGAGTCCTTGAATGATGAGGAATTCCATGCCAC 5 0.125 No Hit GGAGAGACTGGTGTGAGAAAAGTACTTCAAATGCTGCGTGAGGAGTTTGA 5 0.125 No Hit CCTCGATCGAGGGAGTTGAAAAGGGTGGCTACATCATTTCAGATAACTCC 5 0.125 No Hit GTCTCAAGTCTGCCGCAGCTTTCCCAGTCAGTACCAGAAAGGCTAATGAC 5 0.125 No Hit ATCGAAACCTTAAATTCCCTGTCATGATAAGCAAACTCTCAGCTCTTTGT 5 0.125 No Hit ATCTGTTCAGAAGGATGGCATAGAACCTGATAAAGGATTGGCTGAACCAC 5 0.125 No Hit GGTCTCGTGACCGGAGTCGGACCATACCAAAACCTCCTGGATCACCTGGC 5 0.125 No Hit CGATCCTGTTGAGAAGATTAAATCCGGCTTCATTCACTTCAAGAAGGAGA 5 0.125 No Hit TGATGTTGTCAATTAGCTTCTTTCGGGTTTCTCATTCCTATTTTTTGAGG 5 0.125 No Hit CACAAACACTGAGCAACAGAGAGGAAGCAAGAAATTTGCAGAGCAGAGAT 5 0.125 No Hit ATTCGGGTGGATATTATACGACCCGAAGATGGGAAACCGATCAGTGCTGG 5 0.125 No Hit CTGTAGTACACTTGAAATCTGAGCCAGTCATGTCTTCTGTCTCTGGTTTT 5 0.125 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 5 0.125 No Hit TGGACATGGTCACCCCAACAGGCACGAGCAACTCTAACATGGGCATACTT 5 0.125 No Hit TTGTGGGTGGTTCTAAGGTCTCATCGAAGATTGGAGTCATTGAGTCACTC 5 0.125 No Hit GTCAGTTCCATCAAAATGGAATCCAAGCAAGGAGAGAGAGTTCCCTGGTC 5 0.125 No Hit GGGAATGATAGATTTGTGGTCTTAAAAGAAGCACATTTGTCAGGGGTTTC 5 0.125 No Hit CAAAAGACCACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAATGT 5 0.125 No Hit CAGGTAGCACCCCTCCTCCATGGCTTGATGGAAGCCTCCCTGGAGACTTT 5 0.125 No Hit GTTGGGGCAGCAGTAGAGTATGCAGTTTTGCATCTGAAGGTGGAATACAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.25 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.55 0.0 0.0 0.0 0.0 84-85 0.55 0.0 0.0 0.0 0.0 86-87 0.625 0.0 0.0 0.0 0.0 88-89 0.775 0.0 0.0 0.0 0.0 90-91 0.8375 0.0 0.0 0.0 0.0 92-93 0.95 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.1625 0.0 0.0 0.0 0.0 98-99 1.2625 0.0 0.0 0.0 0.0 100-101 1.5750000000000002 0.0 0.0 0.0 0.0 102-103 1.8375 0.0 0.0 0.0 0.0 104-105 2.2625 0.0 0.0 0.0 0.0 106-107 2.55 0.0 0.0 0.0 0.0 108-109 2.8499999999999996 0.0 0.0 0.0 0.0 110-111 3.075 0.0 0.0 0.0 0.0 112-113 3.325 0.0 0.0 0.0 0.0 114-115 3.5 0.0 0.0 0.0 0.0 116-117 3.8 0.0 0.0 0.0 0.0 118-119 4.0875 0.0 0.0 0.0 0.0 120-121 4.625 0.0 0.0 0.0 0.0 122-123 5.2625 0.0 0.0 0.0 0.0 124-125 5.5 0.0 0.0 0.0 0.0 126-127 5.95 0.0 0.0 0.0 0.0 128-129 6.425000000000001 0.0 0.0 0.0 0.0 130-131 6.987500000000001 0.0 0.0 0.0 0.0 132-133 7.575 0.0 0.0 0.0 0.0 134-135 8.5 0.0 0.0 0.0 0.0 136-137 8.850000000000001 0.0 0.0 0.0 0.0 138-139 9.3375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGGGG 65 2.0128899E-5 22.33106 145 >>END_MODULE Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716530 spots for SRR13695409.sra Written 716530 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra Read 716525 spots for SRR13695409.sra Written 716525 spots for SRR13695409.sra SRR ids: ['SRR13695409.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_h9pphfpe SRR13695409.sra spots: 14330505 blocks: [[1, 716525], [716526, 1433050], [1433051, 2149575], [2149576, 2866100], [2866101, 3582625], [3582626, 4299150], [4299151, 5015675], [5015676, 5732200], [5732201, 6448725], [6448726, 7165250], [7165251, 7881775], [7881776, 8598300], [8598301, 9314825], [9314826, 10031350], [10031351, 10747875], [10747876, 11464400], [11464401, 12180925], [12180926, 12897450], [12897451, 13613975], [13613976, 14330505]] SRR13695409 file size 4848432 SRR13695409 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695409 SRR13695409_1.fastq SRR13695409_2.fastq Input file: SRR13695409_1.fastq Paired file: SRR13695409_2.fastq trimmed: SRR13695409-trimmed-pair1.fastq, SRR13695409-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:53:38 2025 >> started Wed Feb 12 00:53:54 2025 >> done (15.740s) 14330505 read pairs processed; of these: 91 ( 0.00%) short read pairs filtered out after trimming by size control 9802 ( 0.07%) empty read pairs filtered out after trimming by size control 14320612 (99.93%) read pairs available; of these: 1908735 (13.33%) trimmed read pairs available after processing 12411877 (86.67%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 0 0.00% 21 0 0.00% 22 1 0.00% 23 0 0.00% 24 0 0.00% 25 4 0.00% 26 2 0.00% 27 3 0.00% 28 1 0.00% 29 1 0.00% 30 1 0.00% 31 3 0.00% 32 4 0.00% 33 3 0.00% 34 4 0.00% 35 10 0.00% 36 4 0.00% 37 7 0.00% 38 10 0.00% 39 14 0.00% 40 26 0.00% 41 33 0.00% 42 11 0.00% 43 26 0.00% 44 33 0.00% 45 24 0.00% 46 29 0.00% 47 36 0.00% 48 37 0.00% 49 63 0.00% 50 61 0.00% 51 74 0.00% 52 99 0.00% 53 96 0.00% 54 109 0.00% 55 102 0.00% 56 112 0.00% 57 134 0.00% 58 188 0.00% 59 186 0.00% 60 271 0.00% 61 249 0.00% 62 347 0.00% 63 342 0.00% 64 421 0.00% 65 453 0.00% 66 472 0.00% 67 570 0.00% 68 576 0.00% 69 705 0.00% 70 834 0.01% 71 929 0.01% 72 1140 0.01% 73 1292 0.01% 74 1456 0.01% 75 1593 0.01% 76 1810 0.01% 77 2048 0.01% 78 2124 0.01% 79 2545 0.02% 80 2638 0.02% 81 3000 0.02% 82 3606 0.03% 83 3884 0.03% 84 4374 0.03% 85 4944 0.03% 86 5171 0.04% 87 5666 0.04% 88 5978 0.04% 89 6335 0.04% 90 6744 0.05% 91 7638 0.05% 92 7623 0.05% 93 8953 0.06% 94 9384 0.07% 95 9995 0.07% 96 11048 0.08% 97 11480 0.08% 98 12089 0.08% 99 12654 0.09% 100 13341 0.09% 101 13588 0.09% 102 14196 0.10% 103 14833 0.10% 104 16264 0.11% 105 16862 0.12% 106 18129 0.13% 107 18493 0.13% 108 19398 0.14% 109 20302 0.14% 110 20481 0.14% 111 21450 0.15% 112 22600 0.16% 113 22933 0.16% 114 23472 0.16% 115 25572 0.18% 116 26148 0.18% 117 26890 0.19% 118 28325 0.20% 119 28561 0.20% 120 29197 0.20% 121 29909 0.21% 122 30650 0.21% 123 31796 0.22% 124 33206 0.23% 125 34058 0.24% 126 35411 0.25% 127 36104 0.25% 128 37200 0.26% 129 38027 0.27% 130 39680 0.28% 131 40000 0.28% 132 40572 0.28% 133 41352 0.29% 134 41588 0.29% 135 42500 0.30% 136 43935 0.31% 137 45149 0.32% 138 45801 0.32% 139 47297 0.33% 140 47819 0.33% 141 48598 0.34% 142 48879 0.34% 143 49943 0.35% 144 50473 0.35% 145 51225 0.36% 146 51995 0.36% 147 53227 0.37% 148 54605 0.38% 149 55542 0.39% 150 56225 0.39% 151 12411877 86.67% 14320612 reads passed initial QC criterion=sequence-density sequence-density=0.66 sequence-density-rank=1 fanout-score=2.11 fanout-score-rank=16 prefix-density=0.67 prefix-fanout=2.1 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=24 fanout-score=193.22 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=10.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.55 sequence-density-rank=1 fanout-score=2.08 fanout-score-rank=16 prefix-density=0.55 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=25 fanout-score=23.29 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=1.9 sequence=CCACCACTAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGGCCTCTCTGCTGACCCAGAGACCTTTGCCAAGAACCGTGAGCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTCTTGGATGCGTCTTCCCCGAGCTCTTGTCCCGCAACGGTGTCAAGTTCGGCGAGGCTGTATGGTTCAAGGCTGGAGCCCAGATCTTCAGCGAGGGTGGACTTGACTACTTGGGCAACCCAAGCTTGATCCAC SRR13695409 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:54:35 Started mapping on | Feb 12 00:54:36 Finished on | Feb 12 00:56:00 Mapping speed, Million of reads per hour | 613.74 Number of input reads | 14320612 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 13633615 Uniquely mapped reads % | 95.20% Average mapped length | 294.31 Number of splices: Total | 13142487 Number of splices: Annotated (sjdb) | 12883000 Number of splices: GT/AG | 12860350 Number of splices: GC/AG | 231636 Number of splices: AT/AC | 7276 Number of splices: Non-canonical | 43225 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 354937 % of reads mapped to multiple loci | 2.48% Number of reads mapped to too many loci | 21236 % of reads mapped to too many loci | 0.15% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.10% % of reads unmapped: other | 0.07% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 332228 332228 332228 N_multimapping 354937 354937 354937 N_noFeature 369936 13342079 506107 N_ambiguous 244896 973 88962 UnstrandedReadsAssigned:13018783 PositiveStrandReadsAssigned:290563 NegativeStrandReadsAssigned:13038546 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695409 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695409-trimmed-pair1.fastq SRR13695409-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,320,612 reads, 13,071,659 reads pseudoaligned [quant] estimated average fragment length: 234.996 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,098 rounds 52401 SRR13695409.ke.tsv 34699 SRR13695409.se.tsv 87100 total ==> SRR13695409.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1784 321.087 11.6602 Potri.005G024800.1.v4.1 1035 801.004 216 17.4703 Potri.004G059700.1.v4.1 961 727.06 14 1.24749 Potri.007G009000.2.v4.1 1416 1182 0 0 Potri.003G141000.2.v4.1 2943 2709 537 12.8424 Potri.016G087400.1.v4.1 270 86.3539 713 534.919 Potri.015G069301.1.v4.1 564 335.269 0 0 Potri.010G195200.1.v4.1 1773 1539 18 0.757727 Potri.012G127500.1.v4.1 977 743.032 74 6.45215 ==> SRR13695409.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 463 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 277 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR13695409 completed mapping pipeline successfully