Starting /dee2/code/volunteer_pipeline.sh SRR13695410 current disk space = 3051253899264 free memory = 1415486956 SRR13695410 SRAfilesize e1a502e84a24510c35bf9e8470a48ced SRR13695410.sra SRR13695410.sra file validated SRR13695410 is paired end SRR13695410 is conventional basespace SRR13695410 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695410_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.601 37.0 37.0 37.0 37.0 37.0 2 36.198 37.0 37.0 37.0 37.0 37.0 3 36.536 37.0 37.0 37.0 37.0 37.0 4 36.6045 37.0 37.0 37.0 37.0 37.0 5 36.575 37.0 37.0 37.0 37.0 37.0 6 36.655 37.0 37.0 37.0 37.0 37.0 7 36.451 37.0 37.0 37.0 37.0 37.0 8 36.499 37.0 37.0 37.0 37.0 37.0 9 36.5895 37.0 37.0 37.0 37.0 37.0 10-14 36.5599 37.0 37.0 37.0 37.0 37.0 15-19 36.4945 37.0 37.0 37.0 37.0 37.0 20-24 36.5366 37.0 37.0 37.0 37.0 37.0 25-29 36.4705 37.0 37.0 37.0 37.0 37.0 30-34 36.4187 37.0 37.0 37.0 37.0 37.0 35-39 36.3861 37.0 37.0 37.0 37.0 37.0 40-44 36.3962 37.0 37.0 37.0 37.0 37.0 45-49 36.381299999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.3352 37.0 37.0 37.0 37.0 37.0 55-59 36.349199999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.293400000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.2924 37.0 37.0 37.0 37.0 37.0 70-74 36.2532 37.0 37.0 37.0 37.0 37.0 75-79 36.23 37.0 37.0 37.0 37.0 37.0 80-84 36.157799999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.1863 37.0 37.0 37.0 37.0 37.0 90-94 36.1113 37.0 37.0 37.0 37.0 37.0 95-99 36.045100000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.076100000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.111900000000006 37.0 37.0 37.0 37.0 37.0 110-114 36.003400000000006 37.0 37.0 37.0 37.0 37.0 115-119 35.9799 37.0 37.0 37.0 37.0 37.0 120-124 35.8886 37.0 37.0 37.0 37.0 37.0 125-129 35.9457 37.0 37.0 37.0 37.0 37.0 130-134 35.95060000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.8837 37.0 37.0 37.0 37.0 37.0 140-144 35.7573 37.0 37.0 37.0 37.0 37.0 145-149 35.636199999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.406499999999994 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 2.0 22 1.0 23 0.0 24 0.0 25 4.0 26 10.0 27 3.0 28 17.0 29 21.0 30 35.0 31 39.0 32 54.0 33 69.0 34 110.0 35 338.0 36 2956.0 37 339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.624999999999996 13.125 10.2 45.050000000000004 2 18.7374245472837 13.606639839034203 35.73943661971831 31.91649899396378 3 18.75 16.675 25.3 39.275 4 22.2 24.85 22.85 30.099999999999998 5 23.575 27.375 25.3 23.75 6 22.325 33.4 23.925 20.349999999999998 7 15.85 27.375 40.1 16.675 8 19.5 27.375 30.2 22.925 9 17.775 22.45 35.725 24.05 10-14 20.06 28.860000000000003 27.705000000000002 23.375 15-19 19.8 27.58 27.900000000000002 24.72 20-24 20.355 28.255000000000003 27.750000000000004 23.64 25-29 20.169999999999998 28.215 27.905 23.71 30-34 20.145 27.905 27.485 24.465 35-39 20.555 27.935 26.640000000000004 24.87 40-44 20.3 28.384999999999998 27.185 24.13 45-49 21.044999999999998 28.07 27.38 23.505000000000003 50-54 19.935 27.794999999999998 28.194999999999997 24.075 55-59 20.419999999999998 28.384999999999998 27.425 23.77 60-64 21.38 27.73 27.765 23.125 65-69 20.66 28.249999999999996 27.715 23.375 70-74 20.985 27.750000000000004 27.605 23.66 75-79 20.745 27.735 27.24 24.279999999999998 80-84 20.715 28.165000000000003 27.405 23.715 85-89 21.455 28.189999999999998 27.13 23.225 90-94 20.385 28.43 27.229999999999997 23.955000000000002 95-99 22.035 27.605 27.05 23.31 100-104 21.335 28.199999999999996 27.005000000000003 23.46 105-109 21.740000000000002 27.445000000000004 27.05 23.765 110-114 21.92 27.77 27.13 23.18 115-119 21.335 28.705000000000002 26.665 23.294999999999998 120-124 21.86 28.38 26.91 22.85 125-129 22.259999999999998 26.96 27.229999999999997 23.549999999999997 130-134 21.310000000000002 28.305000000000003 26.965 23.419999999999998 135-139 21.529999999999998 27.68 26.605 24.185000000000002 140-144 21.845 28.175 26.205000000000002 23.775 145-149 21.605 27.305 26.405 24.685000000000002 150-151 22.25 27.3125 26.987499999999997 23.45 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.5 22 0.5 23 0.0 24 0.0 25 1.0 26 2.0 27 2.0 28 6.5 29 9.0 30 11.0 31 20.5 32 33.0 33 36.0 34 43.0 35 65.0 36 77.0 37 89.5 38 118.5 39 138.0 40 169.0 41 212.0 42 217.5 43 227.5 44 281.0 45 287.0 46 248.0 47 243.0 48 251.5 49 234.5 50 191.5 51 161.0 52 135.0 53 115.0 54 96.5 55 72.5 56 53.5 57 40.0 58 26.5 59 18.5 60 19.5 61 18.0 62 13.0 63 4.5 64 2.0 65 1.0 66 1.5 67 3.5 68 2.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.6 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.2 #Duplication Level Percentage of deduplicated Percentage of total 1 71.37096774193549 48.675000000000004 2 17.595307917888565 24.0 3 6.744868035190615 13.8 4 2.4560117302052786 6.7 5 1.3929618768328444 4.75 6 0.21994134897360706 0.8999999999999999 7 0.1466275659824047 0.7000000000000001 8 0.0 0.0 9 0.036656891495601175 0.22499999999999998 >10 0.036656891495601175 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCACCATTAGCAACATGGAAGTCATAAAGGACAGTCTTTTTCAGCTCAG 10 0.25 No Hit CCCATGGTGTTCTGATGGAGAACTGGCTTTCTCTCTTATGGAAGAGTGGA 9 0.22499999999999998 No Hit GCCACATGGGATAATTCCATGGTTCCAATGCCCCAGAAGCTTGGTGGCCC 7 0.17500000000000002 No Hit CTCAGGTACTTGTCAGCCAATTGGACTCTCTTCACATTCTCTTGCTCCTG 7 0.17500000000000002 No Hit TCTGGTTTATACCTCGATTTGTCATATAACTCCATGGACTCCCACCTCGG 7 0.17500000000000002 No Hit CTCCAATTCAAGTAAGGCTTGCTGCTCTCCCGGAGCCCCCTCAAAAGTGT 7 0.17500000000000002 No Hit GTTGAGTAAACTTTTAGAATTTGGCAATATAATTTCTGGTGGTAAACCCT 6 0.15 No Hit GGGTTAAGGTGTTAATCACCTGATTACATGAAATCGCTGCTTTAGTGGTG 6 0.15 No Hit CCCACATCACCAACCTCCTTCAAGATAAACTTTGCCATTCCTGCACCCAT 6 0.15 No Hit GCTTTGGTTTAGGAGCGGGCTTCTTCTTGGAAAAGAGAGCAACAGTCTTG 6 0.15 No Hit CGATCATGCGCTTCACCTCATCTAGGTGGCTTCCTTTTAGTGATTCTGCC 6 0.15 No Hit TTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAA 6 0.15 No Hit TTCGTGGTTTTTGCTGCTGGAGCTGGGTTGGAGCTGCGGAAGAAGGGAGC 5 0.125 No Hit CTAGTCAATTGTTCAGATACTATTTCATCTGCTAAAAGGTCTGAATCAGG 5 0.125 No Hit TTTCCCTCCATGGAACAGCTCACTCTTCCCTTCTTTGCCATTGCTGGCAA 5 0.125 No Hit GCCGGCAATCCACAGATAATTTTAGTATCTCAACAGCGAGTGTATTTAGG 5 0.125 No Hit GTTTAACCAGGTAGATACCGACCCAGATTGGCAGTGAGCATAGCAAGAAT 5 0.125 No Hit GTCTGATATTGCCACGAGGCATTAAAGGGCTTTCTTCACATCAAAGTAAT 5 0.125 No Hit ATATGATGTCCGACATCTTTCATATGTTCGCTCGAGGAAAGTTTGTACAT 5 0.125 No Hit CCACTTTCCAATTCTCCGGCACTCATCATGATTGGGCTAATTCCCATCTT 5 0.125 No Hit CTGCTTTATCACTTCATGAGCTTCCGAAAGCTTTCCAGCATTCCGCAAAT 5 0.125 No Hit ACTGTCTTAATTTGTTCAGCACATCAGTTGCATCGAACCCAGCATTATCA 5 0.125 No Hit TAAAGTTTTAGAGGTATTCGAGATCAGAACAACGTAAATCTTTGGTCAGA 5 0.125 No Hit CCGCAAACCAAGGTTTTGCATATAGGACAGAATCACCGCCACGAGCACCA 5 0.125 No Hit CTCGGGCTTCATGTAAGGTTCATCCTCAAAGACATCAAGGCCAACACGAA 5 0.125 No Hit CCCACTTGTTGCGAAGAAGGTACTCAATTTCCTGGGCCAATTGCTCAGTA 5 0.125 No Hit CCCAGATCCTATAGTAACATTGGATGCTTGTGCTCTAAATGTGTCGCCTC 5 0.125 No Hit CTCTAATCTGAGCTCTTAATTTTCTAATTGTAGATTCTTGAGCAGCCTGC 5 0.125 No Hit GCCAATTGTTGTTACTATAATATGACCTGTCTCTGTACCATTACCATCCA 5 0.125 No Hit CTCATGATCATCAGGAAACATAGCTGGAGACGGTGGAATGATGACATCTT 5 0.125 No Hit CTGGAATCTCAATATTGGCTGTCATTACTGGTGGAGCAACATACTCCTCA 5 0.125 No Hit CACCGGTACTTGCTGAGCTGCTCCAGCAGTGCATCCTAGGCCTCTAAAGG 5 0.125 No Hit CTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTC 5 0.125 No Hit CTCGGTTTCCAGACCAGTTTGTCCAAAGACATTTTCAATCTGGTGAACTG 5 0.125 No Hit GGACGGCGGAGGAGCAGGCGGGCTAGGTGGAGGAGTGGGATAGTGTGGCC 5 0.125 No Hit CGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGTTATCTCGTAT 5 0.125 TruSeq Adapter, Index 6 (97% over 37bp) TGCCCGAGGAACCTGCCACAGCAGAACCATTTGTTTGACCAGTTATCGGT 5 0.125 No Hit GTTCTCAGGTGGATAAATAGGCTCGATCTCACGAACAATGCAACCTTGAG 5 0.125 No Hit GGAGGAATCTCTGAGTTTTTGAAGAGAGACAGAAGGAGAAAGGGAGTCTT 5 0.125 No Hit GATTGTTTCATTGAAGAGAACTGGTTCTTGGCTCACAAGCCCCATTTGCT 5 0.125 No Hit CTCCTGCAGTGCGATTTAGTTCCTTTCCCCTTGATATCTGATACTCATTG 5 0.125 No Hit CACGGATATGTTAGCAAGTTTAGTAGTGAACAATTGATTGATTCCTTCTC 5 0.125 No Hit GGTCGCACAGGTGTCTTTGATGGACTCACCCTTATCGGCAATGAACCCAG 5 0.125 No Hit TCTGACAGTAAACAAATGATACATATCACACTGCAAACCAGATTGATGCC 5 0.125 No Hit ATGAACAGGAACAATGAGGATTACCGTTCTCCAGCAGTTCCAGCAATGAC 5 0.125 No Hit CTTCAAATCACCTTGCTCCAGTCGTTGGATAGTTTCAGCAAGCTTTTGAA 5 0.125 No Hit CTGGTGAACATAGTCTTTTCCAGGAAATAAAGGTTGCCCAGTCATGATTT 5 0.125 No Hit GCCAAACTTCTCTGCTGCTGAAAGTAAGCCTGCTTTCTCAGCTTTGGTTA 5 0.125 No Hit CGTCGACGTCGGGAACTAGCTCAAACATGCTCGAGCACCCCTTTTATTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.425 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.65 0.0 0.0 0.0 0.0 94-95 0.8374999999999999 0.0 0.0 0.0 0.0 96-97 1.05 0.0 0.0 0.0 0.0 98-99 1.275 0.0 0.0 0.0 0.0 100-101 1.5750000000000002 0.0 0.0 0.0 0.0 102-103 1.7625000000000002 0.0 0.0 0.0 0.0 104-105 1.925 0.0 0.0 0.0 0.0 106-107 2.125 0.0 0.0 0.0 0.0 108-109 2.3375 0.0 0.0 0.0 0.0 110-111 2.65 0.0 0.0 0.0 0.0 112-113 2.8499999999999996 0.0 0.0 0.0 0.0 114-115 3.2 0.0 0.0 0.0 0.0 116-117 3.5875000000000004 0.0 0.0 0.0 0.0 118-119 4.1125 0.0 0.0 0.0 0.0 120-121 4.625 0.0 0.0 0.0 0.0 122-123 5.2625 0.0 0.0 0.0 0.0 124-125 5.8875 0.0 0.0 0.0 0.0 126-127 6.1875 0.0 0.0 0.0 0.0 128-129 6.9375 0.0 0.0 0.0 0.0 130-131 7.55 0.0 0.0 0.0 0.0 132-133 8.3625 0.0 0.0 0.0 0.0 134-135 9.0375 0.0 0.0 0.0 0.0 136-137 9.587499999999999 0.0 0.0 0.0 0.0 138-139 10.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695410 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695410_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.20675 37.0 37.0 37.0 37.0 37.0 2 36.322 37.0 37.0 37.0 37.0 37.0 3 36.0565 37.0 37.0 37.0 37.0 37.0 4 36.251 37.0 37.0 37.0 37.0 37.0 5 36.4205 37.0 37.0 37.0 37.0 37.0 6 36.2675 37.0 37.0 37.0 37.0 37.0 7 36.3075 37.0 37.0 37.0 37.0 37.0 8 36.387 37.0 37.0 37.0 37.0 37.0 9 36.284 37.0 37.0 37.0 37.0 37.0 10-14 36.310500000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.233799999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.20875 37.0 37.0 37.0 37.0 37.0 25-29 36.232549999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.105399999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.100049999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.05965 37.0 37.0 37.0 37.0 37.0 45-49 36.0762 37.0 37.0 37.0 37.0 37.0 50-54 36.03025 37.0 37.0 37.0 37.0 37.0 55-59 36.051249999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.02835 37.0 37.0 37.0 37.0 37.0 65-69 35.9676 37.0 37.0 37.0 37.0 37.0 70-74 35.94475 37.0 37.0 37.0 37.0 37.0 75-79 36.000249999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.9388 37.0 37.0 37.0 37.0 37.0 85-89 35.8432 37.0 37.0 37.0 37.0 37.0 90-94 35.83515 37.0 37.0 37.0 37.0 37.0 95-99 35.8387 37.0 37.0 37.0 37.0 37.0 100-104 35.836149999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.8137 37.0 37.0 37.0 37.0 37.0 110-114 35.754949999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.74875 37.0 37.0 37.0 37.0 37.0 120-124 35.70465 37.0 37.0 37.0 37.0 37.0 125-129 35.7426 37.0 37.0 37.0 37.0 37.0 130-134 35.58705 37.0 37.0 37.0 37.0 37.0 135-139 35.50930000000001 37.0 37.0 37.0 37.0 37.0 140-144 35.50515 37.0 37.0 37.0 34.6 37.0 145-149 35.312 37.0 37.0 37.0 34.6 37.0 150-151 35.19325 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 3.0 15 1.0 16 3.0 17 2.0 18 5.0 19 0.0 20 2.0 21 2.0 22 5.0 23 4.0 24 6.0 25 6.0 26 5.0 27 7.0 28 19.0 29 15.0 30 32.0 31 37.0 32 48.0 33 81.0 34 181.0 35 550.0 36 2735.0 37 249.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.518193224592224 22.409033877038894 14.504391468005018 31.56838143036386 2 29.575000000000003 24.7 27.700000000000003 18.025 3 21.349999999999998 27.575 29.525000000000002 21.55 4 22.5 32.300000000000004 25.775 19.425 5 27.275 34.1 21.75 16.875 6 20.674999999999997 39.475 22.225 17.625 7 20.65 23.275000000000002 36.275 19.8 8 22.125 26.55 26.55 24.775 9 22.625 24.175 31.374999999999996 21.825 10-14 23.34 29.885 26.515 20.26 15-19 23.09 27.715 27.200000000000003 21.995 20-24 23.476173808690433 27.961398069903492 27.44637231861593 21.11605580279014 25-29 23.31082770692673 27.73693423355839 27.38184546136534 21.57039259814954 30-34 22.757275727572758 27.982798279827982 28.277827782778274 20.982098209820983 35-39 23.666183309165458 27.67638381919096 26.82134106705335 21.83609180459023 40-44 23.133470020503076 28.349252387858183 27.154073110966642 21.363204480672103 45-49 22.667266726672665 28.15781578157816 27.34773477347735 21.827182718271825 50-54 22.95614780739037 28.536426821341067 26.911345567278367 21.5960798039902 55-59 23.12578144536134 28.017004251062765 27.016754188547136 21.840460115028755 60-64 21.791089554477725 27.841392069603483 27.951397569878495 22.4161208060403 65-69 23.580000000000002 26.155 28.49 21.775 70-74 23.265816454113526 27.376844211052763 27.79194798699675 21.56539134783696 75-79 23.488523278491773 27.669150372555883 27.414112116817524 21.42821423213482 80-84 24.015 27.355 27.32 21.310000000000002 85-89 23.439687937587518 28.125625125025007 26.915383076615324 21.519303860772155 90-94 23.546177308865442 27.67638381919096 28.116405820291014 20.661033051652584 95-99 24.099999999999998 27.445000000000004 27.3 21.154999999999998 100-104 24.241060265066267 26.95173793448362 27.836959239809957 20.97024256064016 105-109 23.39467893578716 28.525705141028208 26.490298059611924 21.589317863572717 110-114 24.70123506175309 27.316365818290915 26.961348067403367 21.02105105255263 115-119 24.348652297844676 27.97919687953193 27.169075361304195 20.5030754613192 120-124 24.42110527631908 28.37209302325581 26.401600400100023 20.80520130032508 125-129 25.600120024004802 27.675535107021403 26.730346069213844 19.99399879975995 130-134 25.34387035462412 28.11984194468064 25.87905767018456 20.65723003051068 135-139 25.902770831249374 27.488246473942183 26.698009402820844 19.910973291987595 140-144 26.57632881644082 27.531376568828442 26.156307815390768 19.735986799339965 145-149 27.11813544063219 26.387916374912475 26.41292387716315 20.08102430729219 150-151 27.151075537768882 27.75137568784392 25.512756378189096 19.5847923961981 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.5 12 1.0 13 0.5 14 0.5 15 1.0 16 1.0 17 1.0 18 0.5 19 0.5 20 0.5 21 0.5 22 1.5 23 2.0 24 1.5 25 0.5 26 0.5 27 2.0 28 5.5 29 8.0 30 5.0 31 11.5 32 26.5 33 37.0 34 50.5 35 50.0 36 63.0 37 103.0 38 126.0 39 158.5 40 202.0 41 218.0 42 225.5 43 250.0 44 261.5 45 264.0 46 261.0 47 252.5 48 243.5 49 215.0 50 185.0 51 152.0 52 133.5 53 119.5 54 98.0 55 75.5 56 46.0 57 27.0 58 24.5 59 29.5 60 19.0 61 7.5 62 7.0 63 3.0 64 1.0 65 1.5 66 1.0 67 1.5 68 1.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.0 98 1.0 99 0.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.01 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.02 110-114 0.005 115-119 0.015 120-124 0.025 125-129 0.02 130-134 0.034999999999999996 135-139 0.03 140-144 0.005 145-149 0.03 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.125 #Duplication Level Percentage of deduplicated Percentage of total 1 71.22935779816514 48.525 2 17.68807339449541 24.099999999999998 3 6.862385321100918 14.025000000000002 4 2.385321100917431 6.5 5 1.3577981651376148 4.625 6 0.29357798165137616 1.2 7 0.07339449541284404 0.35000000000000003 8 0.03669724770642202 0.2 9 0.03669724770642202 0.22499999999999998 >10 0.03669724770642202 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTCTTTTGAGCTCTCCAAGATTTGCTTTCCTTTGAACAACACTCACCAC 10 0.25 No Hit CCGCCACAAATCTCGGAGACAGGTCTGAGAAACAAGTGGGGCAAACCTAA 9 0.22499999999999998 No Hit CTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 8 0.2 No Hit CGTGTATGAAGGATGTTATAAGATTGTCAAATCGGTTAAATGAGAAGCCA 7 0.17500000000000002 No Hit GCCTGAAAACCATGGTGTCCCATATTCATTAACTGAGGAGTTTGTCCCTG 7 0.17500000000000002 No Hit AGTGTTTGTGCAACCTCTTAATTCGACAGGGTACCGAAGAAGAATGGCAT 6 0.15 No Hit GGAAGATATTGTCAAGCTTGTTGATACCTTCCCTGGTCAATCTATCGATT 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 6 0.15 No Hit GAACCTCACTGGGCTTTCTGTTATTCTATGCTCCCTAGAGTCTCTCGTAG 6 0.15 No Hit GTGAAGCTTCTAATCTGCTAGCTCCAATAGATTCATCAATCATGGCAGGT 6 0.15 No Hit ATCTTAATCTTGCATGCTGAGCAGCCCTCTTGTATTCTTACACACACGAG 6 0.15 No Hit CACAACAGAAGCGCCTGAGCAGCTCGAAAGACAGAAAGCTTTGCAACAAA 6 0.15 No Hit CGCTAAGATGGAATTTCAATTTCAGGTCCGTAGTTCTGTTACTGGTGCAT 6 0.15 No Hit CCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGGAAAAGTCACACTAGAG 5 0.125 No Hit CTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAA 5 0.125 No Hit CTGAGGTCAGAAAGCAATGGAGGAGCGACTGAAACACTTGACATTAGTTG 5 0.125 No Hit GTGGAACAGGCTTTGCTGGCCATGGGGATCATCTCATGGCAACAGAGTTC 5 0.125 No Hit CGTAATTGATGAGGTTCTTGGGACTTGTGAGATTTTCGAGGAAAAGCAAG 5 0.125 No Hit AGAGAACCTTGGCATGACATCCATTCCCGGCTTGAGGGGCCTATTGCTTG 5 0.125 No Hit GCCTGCCTAATATCAAGGTTCCTCTCATCTTGGGTATTTGGGGAGGCAAA 5 0.125 No Hit TGACTGGCGAAGTATATCAAGAAACTTTGTGGTGACAAGAACACCTACCC 5 0.125 No Hit GGGGCTGACTCTTCCAAAAACACCACTAACTTCAAGTAGCAGCAGCAGCG 5 0.125 No Hit AGATAAGGTTGCTTCAATGCTGCCGAAAAAGTATTCCCAGCTTGGGAATG 5 0.125 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 5 0.125 No Hit TGATACTCTTGCTAAAGCAATTATTGGAACAATTGGAGACGTGGATTCAT 5 0.125 No Hit AGCTACACAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTT 5 0.125 No Hit AGCAGATCAACCTTTCCGTTTTCCTGCCACTTTCACATTTGTTGTGAGAG 5 0.125 No Hit AAGAAATGTTTGAAGACATGATAGTCAACGGCCAATTACCAAATGTATTT 5 0.125 No Hit CTCAAACTAGTTAGTTGATTTTAGTTTGAGCGGTAGAAATTAATATTGGA 5 0.125 No Hit CTTCATCAGATTTTTCCCTTCTTCTCGCCAGCCTAGAGCTTGGCTCTTTG 5 0.125 No Hit GTTGTGAGTTAGCAAAAGACTCATGGACGCAAGTTCTAGTGATTCTAAGT 5 0.125 No Hit TGGTTGGGATGTTTGGCGAAATGATATACGCAAGGGTTTTTGGTAACTCG 5 0.125 No Hit TGGAAAGCAGAGGGAAACAGATCTAAAAAATAACTAAAACAGACTGCTTG 5 0.125 No Hit CTATCTATGAGGGTAACAGGGTGTGCTTGGTATCAAGGTCAAGATCATGC 5 0.125 No Hit TAGAATATCGTGTTGACTGCCAGCTGTTACAACATCTACATTCAGAAAAC 5 0.125 No Hit ACTGGACTTGGCAGCTCTTCAACAAGAGGATTGTGGATAGATTCTTGCTA 5 0.125 No Hit GGCGAAGGCTGATGAGATTGCGAAGTTGATGAATGAAAATGAGCACCTGA 5 0.125 No Hit GGAGGAACATCTAGTCTGTTATTTGAAGCATCGTCATCGTCATCATCTAT 5 0.125 No Hit TCTTGTTCCCCCTCTCGAGAACGGACGGACAGAAACTAATGCTGCCCTTG 5 0.125 No Hit CATGGTTAATAAATATTATGATCTTGTTACCAGCTTTTATGAGTTTGGCT 5 0.125 No Hit AAAACCCAGAAGCCAACAAGAGCTGCAACCCATTTCAACTGAAACAGATC 5 0.125 No Hit CATAATGCTGGCGTGGACAAGTTACCTGAGGAAATGAATGACATGAAAAT 5 0.125 No Hit CGGGAAGGTTTCCACAGTTAACACTGGCTATTCTAAGAGGGCCTACGTGA 5 0.125 No Hit GCCTGTGGTTGGGATGATAAATGGAGTTCCTGAGGTAGTCTTGAAAATCT 5 0.125 No Hit AATCTCACCACATATGGACCTTTTGGAAATGCTACCGGCACTTCTTTCTC 5 0.125 No Hit TGATGAGTCGGGAACAACATTAGACAATGTGAAGGGAGAAATTGAACTTC 5 0.125 No Hit CACGGATCTACATCAAATAATACGCTCCAACCAACTATTGACAGATGATC 5 0.125 No Hit ACAATGAAGAAGGAAGCAATCCTTGTAAACTGCAGTAGGGGTCCAGTGGT 5 0.125 No Hit TTTTGCGCTGTTTTTGTTATTGTCTGCCTTTCTATGCTTTACTGGCTAAG 5 0.125 No Hit CAAAGCTTTTCCAAAATCCAGAAGATGGAGGCCACAGCATTGTGTGGCAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.45 0.0 0.0 0.0 0.0 90-91 0.5875 0.0 0.0 0.0 0.0 92-93 0.675 0.0 0.0 0.0 0.0 94-95 0.8625 0.0 0.0 0.0 0.0 96-97 1.075 0.0 0.0 0.0 0.0 98-99 1.2999999999999998 0.0 0.0 0.0 0.0 100-101 1.6 0.0 0.0 0.0 0.0 102-103 1.8 0.0 0.0 0.0 0.0 104-105 1.9749999999999999 0.0 0.0 0.0 0.0 106-107 2.175 0.0 0.0 0.0 0.0 108-109 2.3875 0.0 0.0 0.0 0.0 110-111 2.7 0.0 0.0 0.0 0.0 112-113 2.9000000000000004 0.0 0.0 0.0 0.0 114-115 3.25 0.0 0.0 0.0 0.0 116-117 3.6125 0.0 0.0 0.0 0.0 118-119 4.1375 0.0 0.0 0.0 0.0 120-121 4.65 0.0 0.0 0.0 0.0 122-123 5.3 0.0 0.0 0.0 0.0 124-125 5.9125 0.0 0.0 0.0 0.0 126-127 6.225 0.0 0.0 0.0 0.0 128-129 7.0 0.0 0.0 0.0 0.0 130-131 7.625 0.0 0.0 0.0 0.0 132-133 8.4375 0.0 0.0 0.0 0.0 134-135 9.1125 0.0 0.0 0.0 0.0 136-137 9.662500000000001 0.0 0.0 0.0 0.0 138-139 10.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221419 spots for SRR13695410.sra Written 1221419 spots for SRR13695410.sra Read 1221437 spots for SRR13695410.sra Written 1221437 spots for SRR13695410.sra SRR ids: ['SRR13695410.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7xijak6y SRR13695410.sra spots: 24428398 blocks: [[1, 1221419], [1221420, 2442838], [2442839, 3664257], [3664258, 4885676], [4885677, 6107095], [6107096, 7328514], [7328515, 8549933], [8549934, 9771352], [9771353, 10992771], [10992772, 12214190], [12214191, 13435609], [13435610, 14657028], [14657029, 15878447], [15878448, 17099866], [17099867, 18321285], [18321286, 19542704], [19542705, 20764123], [20764124, 21985542], [21985543, 23206961], [23206962, 24428398]] SRR13695410 file size 8280138 SRR13695410 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695410 SRR13695410_1.fastq SRR13695410_2.fastq Input file: SRR13695410_1.fastq Paired file: SRR13695410_2.fastq trimmed: SRR13695410-trimmed-pair1.fastq, SRR13695410-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 00:47:40 2025 >> started Wed Feb 12 00:48:22 2025 >> done (42.015s) 24428398 read pairs processed; of these: 156 ( 0.00%) short read pairs filtered out after trimming by size control 9229 ( 0.04%) empty read pairs filtered out after trimming by size control 24419013 (99.96%) read pairs available; of these: 3235357 (13.25%) trimmed read pairs available after processing 21183656 (86.75%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 1 0.00% 21 1 0.00% 22 3 0.00% 23 0 0.00% 24 3 0.00% 25 2 0.00% 26 0 0.00% 27 7 0.00% 28 2 0.00% 29 13 0.00% 30 7 0.00% 31 4 0.00% 32 9 0.00% 33 14 0.00% 34 4 0.00% 35 9 0.00% 36 13 0.00% 37 22 0.00% 38 11 0.00% 39 21 0.00% 40 15 0.00% 41 18 0.00% 42 17 0.00% 43 30 0.00% 44 29 0.00% 45 34 0.00% 46 44 0.00% 47 43 0.00% 48 53 0.00% 49 77 0.00% 50 70 0.00% 51 101 0.00% 52 115 0.00% 53 143 0.00% 54 123 0.00% 55 139 0.00% 56 166 0.00% 57 180 0.00% 58 235 0.00% 59 298 0.00% 60 351 0.00% 61 361 0.00% 62 410 0.00% 63 487 0.00% 64 585 0.00% 65 558 0.00% 66 714 0.00% 67 775 0.00% 68 864 0.00% 69 1115 0.00% 70 1194 0.00% 71 1322 0.01% 72 1637 0.01% 73 1807 0.01% 74 2095 0.01% 75 2387 0.01% 76 2514 0.01% 77 2932 0.01% 78 3148 0.01% 79 3753 0.02% 80 4214 0.02% 81 4787 0.02% 82 5392 0.02% 83 5864 0.02% 84 6917 0.03% 85 7576 0.03% 86 8091 0.03% 87 8636 0.04% 88 9890 0.04% 89 10415 0.04% 90 11409 0.05% 91 12390 0.05% 92 13459 0.06% 93 14382 0.06% 94 16160 0.07% 95 17462 0.07% 96 18642 0.08% 97 19382 0.08% 98 20763 0.09% 99 21846 0.09% 100 23099 0.09% 101 24508 0.10% 102 25445 0.10% 103 27001 0.11% 104 28406 0.12% 105 30229 0.12% 106 31743 0.13% 107 33515 0.14% 108 34913 0.14% 109 35993 0.15% 110 36861 0.15% 111 38548 0.16% 112 39628 0.16% 113 41023 0.17% 114 42975 0.18% 115 44957 0.18% 116 46377 0.19% 117 48164 0.20% 118 49782 0.20% 119 50463 0.21% 120 52324 0.21% 121 53077 0.22% 122 55062 0.23% 123 55826 0.23% 124 56878 0.23% 125 58858 0.24% 126 61456 0.25% 127 62510 0.26% 128 64310 0.26% 129 64705 0.26% 130 66283 0.27% 131 67824 0.28% 132 67835 0.28% 133 70790 0.29% 134 70426 0.29% 135 71891 0.29% 136 73146 0.30% 137 74741 0.31% 138 75592 0.31% 139 78488 0.32% 140 78723 0.32% 141 80378 0.33% 142 81479 0.33% 143 82578 0.34% 144 82805 0.34% 145 84946 0.35% 146 85047 0.35% 147 86000 0.35% 148 88874 0.36% 149 90067 0.37% 150 90044 0.37% 151 21183656 86.75% 24419013 reads passed initial QC criterion=sequence-density sequence-density=0.59 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=19 prefix-density=0.60 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=21 fanout-score=380.00 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=15.2 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=sequence-density sequence-density=0.79 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=20 prefix-density=0.79 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=106.46 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=6.9 sequence=ACAACTTCAAGGGCAGTAGTCTTAAACCATACTCTAAAATCTTCTTATAATTCCAGTTGTAATATTCTGCTAGCATATAATGGCTTCTTCAATGAGCTTGAAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCACCACTAGCAGAAGCTGCCATCTCATGTGGCCAGGTGTCAAGCAGCTTGGCACAATGTATAACCTACCTCCAGAAGGGTGGGGCTGTGCCTGCAGCTTGCTGCAGTGGGTTGAAAGGACTTAATTCTGCAGCCACGACCACCGCCGACCGCCAAGGGGTCTGCAACTGTTTGAAATCCTTGGCTGGTAAGATCTCTGGCATCAACTATGGCGTGGCTGCTGGCCTCCCTTCAAAGTGTGGTGTATCCATCTCCTACAAAATCAGTCCTTCCACAGA SRR13695410 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 00:49:58 Started mapping on | Feb 12 00:49:58 Finished on | Feb 12 00:54:22 Mapping speed, Million of reads per hour | 332.99 Number of input reads | 24419013 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 20234442 Uniquely mapped reads % | 82.86% Average mapped length | 288.23 Number of splices: Total | 20217649 Number of splices: Annotated (sjdb) | 19833531 Number of splices: GT/AG | 19801275 Number of splices: GC/AG | 347267 Number of splices: AT/AC | 11715 Number of splices: Non-canonical | 57392 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 509905 % of reads mapped to multiple loci | 2.09% Number of reads mapped to too many loci | 28536 % of reads mapped to too many loci | 0.12% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 14.87% % of reads unmapped: other | 0.06% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3675198 3675198 3675198 N_multimapping 509905 509905 509905 N_noFeature 527985 19826799 735737 N_ambiguous 423089 4579 219401 UnstrandedReadsAssigned:19283368 PositiveStrandReadsAssigned:403064 NegativeStrandReadsAssigned:19279304 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695410 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695410-trimmed-pair1.fastq SRR13695410-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,419,013 reads, 21,578,151 reads pseudoaligned [quant] estimated average fragment length: 230.082 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,114 rounds 52401 SRR13695410.ke.tsv 34699 SRR13695410.se.tsv 87100 total ==> SRR13695410.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1788.92 526 12.6283 Potri.005G024800.1.v4.1 1035 805.918 238 12.6834 Potri.004G059700.1.v4.1 961 731.986 25 1.46685 Potri.007G009000.2.v4.1 1416 1186.92 0 0 Potri.003G141000.2.v4.1 2943 2713.92 1006 15.9202 Potri.016G087400.1.v4.1 270 92.7164 1111 514.643 Potri.015G069301.1.v4.1 564 341.894 0 0 Potri.010G195200.1.v4.1 1773 1543.92 24 0.667629 Potri.012G127500.1.v4.1 977 747.939 367 21.0741 ==> SRR13695410.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 335 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 283 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 4 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 9 SRR13695410 completed mapping pipeline successfully