Starting /dee2/code/volunteer_pipeline.sh SRR13695411 current disk space = 3051170566144 free memory = 1514113116 SRR13695411 SRAfilesize 0cc53afdfe6d4ecfab6eb58f1ade828b SRR13695411.sra SRR13695411.sra file validated SRR13695411 is paired end SRR13695411 is conventional basespace SRR13695411 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695411_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.582 37.0 37.0 37.0 37.0 37.0 2 36.37075 37.0 37.0 37.0 37.0 37.0 3 36.6315 37.0 37.0 37.0 37.0 37.0 4 36.6435 37.0 37.0 37.0 37.0 37.0 5 36.648 37.0 37.0 37.0 37.0 37.0 6 36.621 37.0 37.0 37.0 37.0 37.0 7 36.462 37.0 37.0 37.0 37.0 37.0 8 36.5995 37.0 37.0 37.0 37.0 37.0 9 36.5415 37.0 37.0 37.0 37.0 37.0 10-14 36.5948 37.0 37.0 37.0 37.0 37.0 15-19 36.5515 37.0 37.0 37.0 37.0 37.0 20-24 36.5774 37.0 37.0 37.0 37.0 37.0 25-29 36.538599999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.477000000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.456 37.0 37.0 37.0 37.0 37.0 40-44 36.461400000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.4423 37.0 37.0 37.0 37.0 37.0 50-54 36.4213 37.0 37.0 37.0 37.0 37.0 55-59 36.4341 37.0 37.0 37.0 37.0 37.0 60-64 36.4275 37.0 37.0 37.0 37.0 37.0 65-69 36.3399 37.0 37.0 37.0 37.0 37.0 70-74 36.3326 37.0 37.0 37.0 37.0 37.0 75-79 36.334500000000006 37.0 37.0 37.0 37.0 37.0 80-84 36.251400000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.338100000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.278000000000006 37.0 37.0 37.0 37.0 37.0 95-99 36.1622 37.0 37.0 37.0 37.0 37.0 100-104 36.2408 37.0 37.0 37.0 37.0 37.0 105-109 36.1991 37.0 37.0 37.0 37.0 37.0 110-114 36.168 37.0 37.0 37.0 37.0 37.0 115-119 36.1602 37.0 37.0 37.0 37.0 37.0 120-124 36.04729999999999 37.0 37.0 37.0 37.0 37.0 125-129 36.020300000000006 37.0 37.0 37.0 37.0 37.0 130-134 36.057700000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.9505 37.0 37.0 37.0 37.0 37.0 140-144 35.90089999999999 37.0 37.0 37.0 37.0 37.0 145-149 35.7136 37.0 37.0 37.0 37.0 37.0 150-151 35.637 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 0.0 22 0.0 23 0.0 24 1.0 25 3.0 26 2.0 27 4.0 28 13.0 29 19.0 30 28.0 31 27.0 32 48.0 33 53.0 34 117.0 35 299.0 36 3000.0 37 384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.449999999999996 14.075 7.249999999999999 37.225 2 20.767494356659142 13.167795334838225 35.214446952595935 30.850263355906698 3 19.225 17.8 25.674999999999997 37.3 4 22.650000000000002 25.2 22.525000000000002 29.625 5 22.225 29.325000000000003 26.375 22.075 6 23.075000000000003 32.95 22.975 21.0 7 14.875 28.95 39.95 16.225 8 15.875 29.275000000000002 31.574999999999996 23.275000000000002 9 17.9 24.85 34.699999999999996 22.55 10-14 19.06 29.794999999999998 28.075 23.07 15-19 19.64 28.549999999999997 27.91 23.9 20-24 19.765 29.035 27.169999999999998 24.03 25-29 19.84 28.42 28.299999999999997 23.44 30-34 19.88 28.249999999999996 27.85 24.02 35-39 19.34 29.095 27.750000000000004 23.815 40-44 19.17 29.67 27.200000000000003 23.96 45-49 20.16 29.13 27.334999999999997 23.375 50-54 19.785 29.145 27.224999999999998 23.845 55-59 19.869999999999997 28.275 27.915 23.94 60-64 20.405 29.07 27.735 22.79 65-69 19.96 28.715000000000003 27.894999999999996 23.43 70-74 20.455000000000002 29.035 27.279999999999998 23.23 75-79 20.395 28.845 27.47 23.29 80-84 20.244999999999997 29.17 27.310000000000002 23.275000000000002 85-89 20.175 28.875 28.044999999999998 22.905 90-94 20.28 27.875 28.08 23.765 95-99 20.02 28.185 28.005000000000003 23.79 100-104 20.205000000000002 28.715000000000003 27.750000000000004 23.330000000000002 105-109 20.455000000000002 28.044999999999998 27.445000000000004 24.055 110-114 20.474999999999998 28.110000000000003 27.32 24.095 115-119 20.835 27.965 27.060000000000002 24.14 120-124 20.695 29.365000000000002 26.215 23.724999999999998 125-129 20.48 28.315 26.99 24.215 130-134 20.515 28.634999999999998 27.310000000000002 23.54 135-139 20.415 28.134999999999998 26.705000000000002 24.745 140-144 20.775 28.925 26.515 23.785 145-149 21.275 28.18 26.25 24.295 150-151 21.075 27.3875 27.1375 24.4 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 0.5 24 2.5 25 4.0 26 6.0 27 7.0 28 6.5 29 14.0 30 19.5 31 29.5 32 39.5 33 49.5 34 63.0 35 80.5 36 94.5 37 119.0 38 165.0 39 185.5 40 198.0 41 195.0 42 205.5 43 247.0 44 246.5 45 275.0 46 264.5 47 220.5 48 229.0 49 209.5 50 165.5 51 143.0 52 117.0 53 84.5 54 74.5 55 60.5 56 46.5 57 26.5 58 20.0 59 26.5 60 23.0 61 12.5 62 6.5 63 4.5 64 3.0 65 2.0 66 0.0 67 1.5 68 1.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.325 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.0 #Duplication Level Percentage of deduplicated Percentage of total 1 68.88059701492537 46.150000000000006 2 19.47761194029851 26.1 3 7.574626865671642 15.225 4 2.5 6.7 5 1.0447761194029852 3.5000000000000004 6 0.2985074626865672 1.2 7 0.1492537313432836 0.7000000000000001 8 0.0373134328358209 0.2 9 0.0373134328358209 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGGTGATTTTCCAAGAGATCTCCCAGAACCCGAAACTGGTTGAATTTC 9 0.22499999999999998 No Hit GTGATGATGGGCGGGATTTGAAGCTTATGAACACAGATGGATGGTGATGA 8 0.2 No Hit CCTGATTACAAACCTTATTGCATAAATACACAAGGAACGGTTTAGTCTTT 7 0.17500000000000002 No Hit GGCCTGTGAAGGATTGAAGGGGACTATCAATAACCCTAGACGTGCCCATT 7 0.17500000000000002 No Hit GGAGCAAGTTGGGAAGCCACCACATCAAAGAAGGATCCCTCACTAGCGTT 7 0.17500000000000002 No Hit GTTGTTGAACGCTCTGACTGAGAAAATCGAATCAAGATGTACATTAGGAG 7 0.17500000000000002 No Hit CTGTCAACTTCCCAGAGTAAGTTATTAGGCATGGGGCTGCTGAACATCCA 6 0.15 No Hit CACTTCTCATTCATTTACAAAATCGATCACAGAATCTACTAGAAGAGCGA 6 0.15 No Hit GAGTTCTCCTCTACAAGGATCTGAAAAAACTGGTACTGTAGTGTTGTAGA 6 0.15 No Hit CTTTGATTGGATATCACGGTGAAGTACCATCCTTGCTAGTATCCACTCTA 6 0.15 No Hit CGACAGTACAATTGGATGTTCAATCGTCCGAGCAATACAAAGCAGAAAAA 6 0.15 No Hit GCCTCCTTTTCACATTGTGTACAGAGGTCTGGGAAAGGTGCATTGGCATG 6 0.15 No Hit ACGGGCTTGATGTAACGGGAGTCTCAAGACTTCCAATGAAGGGATCGCCA 6 0.15 No Hit CCGGCTCAGTATTCTTCCAAGAGGTGTGGAGTCATAGAAAGACATGGGTG 6 0.15 No Hit ACACAATTGATCAAGAAAGGCTCTGTCATGAGATATGATGACCATCGGCA 5 0.125 No Hit GAGAATCGAAAGGAAAAAAGAAAATTCAAGCTTTCGGAAATTTCAGAAGT 5 0.125 No Hit GACGTCTAAGATTGAAGTTGAAGAAGCATCTATGATGGGAATGTCAATGA 5 0.125 No Hit GTGATCACATAAAACTCCTCTACCTGGGCTTTCCTCAACAGTCCACCGGT 5 0.125 No Hit GCATTGAATAGATCGGCGGATAAGAATATTTGTCCGTCTGTAATGGAAAT 5 0.125 No Hit CGCCAACAGAGAATAAAGGGGGGAAAGCTGAAGCGGACGGCAGCACTGAT 5 0.125 No Hit CCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTC 5 0.125 No Hit GCCAGCAGTGTCCCAGCCGTAGTCACCAGGGAACTCACCAGTCAAGTAGG 5 0.125 No Hit GTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGC 5 0.125 No Hit GTAACCAAATGGGGTATACTGAGCGTTGCAGGCTCGGTGGCTCTCAACCT 5 0.125 No Hit GTTGAGCATATCAGTGTGTTACCTTTCATGAACTGGATGCCCTGTGTCAG 5 0.125 No Hit GACGAGTATTGCATTGCATTTTACAACAGTAAAGCATGCATGCAAGCAAA 5 0.125 No Hit GCACCCAATTCTTCTCCTGACCAAACTATAGCTTTCATTTTCACAAGATC 5 0.125 No Hit GGACTATCTTCTTTGACTTTTACACGCGTTGCTATATCCACATCAACCTG 5 0.125 No Hit GTTCTTGTTAATGTCAGTATTGAAAAGGGTCTCCATTTCATCAAAATCAG 5 0.125 No Hit CCCTTCTCCATTCCCTTCAACCCACACATATAGACATAAGTGTTGTCTTT 5 0.125 No Hit CTTCAATTGCACTTCCTGCACGAGCAATGCCAGAGAGGATTCTTCGTCGG 5 0.125 No Hit GTTTGCGAAGATGACGTTCCCGTCTTTTTCTGATATCTATACCGGCTAAA 5 0.125 No Hit GTAGGTTGATCCTTATTTTCTGTGGTGAAATCCACATCCTTCGGATGGGA 5 0.125 No Hit CACATGTTCTTCCCCATCTCCATCTTAAAGTAACCGTTGTCGCCCCAGTC 5 0.125 No Hit ATAGTATTTTCGCTACAGGGCCCCGCTGTTGTTGTACATTATTTATTGTT 5 0.125 No Hit ATGCTAATCGAGTCTGGCTCTAGCTCAAACAAATTGTCTAGGTCAGGCGA 5 0.125 No Hit GTCTCTTGCCTCATATGGGTAGTCCATCCACCTGTAGTCCCATTCATAAT 5 0.125 No Hit GCTGCATAAGATATGGGTGCAGAAATGGCATCTTGTGGTACCCACTGATG 5 0.125 No Hit CATAAATATAGAACCAAAATCACCGGATTGGTTCTAGAAGAGGATTGTAG 5 0.125 No Hit AGCAGCGGCTTCGTCGATGTTGAATTTCATGGGGAAATTCCAAAGAGGGT 5 0.125 No Hit GCACTTTCTACTCAAGTTCAAATTTTTGTTCCATGTGGTACAGTTTGTGA 5 0.125 No Hit GTCCTGAGTGTGTATTGGCTGGTGTGGTTGCTGATCTTGTGCCATTGCAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.3 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.4 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.7375 0.0 0.0 0.0 0.0 94-95 0.9874999999999999 0.0 0.0 0.0 0.0 96-97 1.1 0.0 0.0 0.0 0.0 98-99 1.225 0.0 0.0 0.0 0.0 100-101 1.5125000000000002 0.0 0.0 0.0 0.0 102-103 1.7 0.0 0.0 0.0 0.0 104-105 1.9874999999999998 0.0 0.0 0.0 0.0 106-107 2.4125 0.0 0.0 0.0 0.0 108-109 2.8375000000000004 0.0 0.0 0.0 0.0 110-111 3.275 0.0 0.0 0.0 0.0 112-113 3.6125 0.0 0.0 0.0 0.0 114-115 3.95 0.0 0.0 0.0 0.0 116-117 4.225 0.0 0.0 0.0 0.0 118-119 4.5125 0.0 0.0 0.0 0.0 120-121 4.975 0.0 0.0 0.0 0.0 122-123 5.625 0.0 0.0 0.0 0.0 124-125 6.1375 0.0 0.0 0.0 0.0 126-127 6.5125 0.0 0.0 0.0 0.0 128-129 6.824999999999999 0.0 0.0 0.0 0.0 130-131 7.3125 0.0 0.0 0.0 0.0 132-133 7.7375 0.0 0.0 0.0 0.0 134-135 8.3875 0.0 0.0 0.0 0.0 136-137 8.975000000000001 0.0 0.0 0.0 0.0 138-139 9.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTATT 10 0.006830828 145.0 7 GTATTCT 10 0.006830828 145.0 9 TCAGTAT 10 0.006830828 145.0 6 CTCAGTA 10 0.006830828 145.0 5 GGCTCAG 10 0.006830828 145.0 3 TTGGTGG 10 0.006830828 145.0 9 CGGCTCA 10 0.006830828 145.0 2 GCTCAGT 10 0.006830828 145.0 4 CCGGCTC 10 0.006830828 145.0 1 >>END_MODULE SRR13695411 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695411_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2595 37.0 37.0 37.0 37.0 37.0 2 36.1585 37.0 37.0 37.0 37.0 37.0 3 36.0425 37.0 37.0 37.0 37.0 37.0 4 36.255 37.0 37.0 37.0 37.0 37.0 5 36.273 37.0 37.0 37.0 37.0 37.0 6 36.2865 37.0 37.0 37.0 37.0 37.0 7 36.245 37.0 37.0 37.0 37.0 37.0 8 36.3405 37.0 37.0 37.0 37.0 37.0 9 36.27 37.0 37.0 37.0 37.0 37.0 10-14 36.309 37.0 37.0 37.0 37.0 37.0 15-19 36.2599 37.0 37.0 37.0 37.0 37.0 20-24 36.24995 37.0 37.0 37.0 37.0 37.0 25-29 36.212650000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.16215 37.0 37.0 37.0 37.0 37.0 35-39 36.18735 37.0 37.0 37.0 37.0 37.0 40-44 36.16415 37.0 37.0 37.0 37.0 37.0 45-49 36.13125 37.0 37.0 37.0 37.0 37.0 50-54 36.049350000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.08265 37.0 37.0 37.0 37.0 37.0 60-64 36.036350000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.01735000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.90725 37.0 37.0 37.0 37.0 37.0 75-79 35.94 37.0 37.0 37.0 37.0 37.0 80-84 35.968050000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.7697 37.0 37.0 37.0 37.0 37.0 90-94 35.8072 37.0 37.0 37.0 37.0 37.0 95-99 35.812400000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.812149999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.83005000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.70745000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.65915 37.0 37.0 37.0 37.0 37.0 120-124 35.57275 37.0 37.0 37.0 37.0 37.0 125-129 35.59005 37.0 37.0 37.0 37.0 37.0 130-134 35.37055 37.0 37.0 37.0 34.6 37.0 135-139 35.38445 37.0 37.0 37.0 37.0 37.0 140-144 35.09285 37.0 37.0 37.0 27.4 37.0 145-149 34.93555 37.0 37.0 37.0 25.0 37.0 150-151 34.516000000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 2.0 14 4.0 15 3.0 16 1.0 17 2.0 18 1.0 19 0.0 20 4.0 21 2.0 22 2.0 23 3.0 24 2.0 25 2.0 26 10.0 27 10.0 28 6.0 29 13.0 30 23.0 31 46.0 32 55.0 33 114.0 34 244.0 35 602.0 36 2647.0 37 199.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.72945891783567 22.019038076152306 11.54809619238477 26.703406813627257 2 28.799999999999997 26.0 29.125 16.075 3 21.075 26.325 32.0 20.599999999999998 4 24.474999999999998 32.45 23.025000000000002 20.05 5 25.575 36.8 21.6 16.025 6 20.549999999999997 40.575 20.974999999999998 17.9 7 22.375 23.75 36.625 17.25 8 20.825 26.174999999999997 29.2 23.799999999999997 9 23.45 25.25 27.575 23.724999999999998 10-14 23.48 29.2 27.055 20.265 15-19 23.285 28.845 26.855 21.015 20-24 23.618266393237633 28.68503976391737 26.94943230130546 20.747261541539537 25-29 23.112334250688015 28.56642481861396 27.965974480860645 20.355266449837377 30-34 23.11771474310871 27.69022962629446 28.375606583620993 20.816449046975837 35-39 23.79332766468264 27.794728154854198 26.67433601760616 21.737608162857 40-44 23.41787983390865 28.38060933513432 27.465105808194508 20.73640502276252 45-49 22.330048521834826 27.752488619878946 28.387774498524337 21.52968835976189 50-54 23.588255889561346 27.92977542139749 27.21952683439204 21.26244185464913 55-59 23.50763072304228 27.98598949211909 27.700775581686266 20.805604203152363 60-64 23.4332016205672 28.499974991246933 26.769369279247734 21.297454108938126 65-69 23.128469270390557 29.014352152822926 26.934040106015907 20.923138470770617 70-74 23.502626970227674 28.03102326745059 27.495621716287218 20.970728046034527 75-79 24.37462477486492 27.636581949169504 27.341404842905742 20.647388433059835 80-84 23.835958989747436 28.56714178544636 26.47161790447612 21.12528132033008 85-89 23.6865806064245 28.179725808065648 27.52927048934254 20.604423096167316 90-94 23.739495798319325 27.516006402561022 27.846138455382153 20.898359343737493 95-99 23.727118135440634 28.388516554966493 27.103130939281783 20.781234370311093 100-104 23.787840880660497 27.920940705529144 27.66574931198399 20.62546910182637 105-109 24.398298724043034 27.995996997748314 27.25544158118589 20.350262697022767 110-114 23.993397689191216 27.544640624218474 28.229880458160356 20.23208122842995 115-119 24.763572679509632 28.136102076557417 27.075306479859897 20.025018764073053 120-124 24.193144858643983 27.960970728046036 27.91593695271454 19.929947460595447 125-129 24.94121178766198 28.138289888427476 26.767398809225995 20.153099514684545 130-134 24.858643982987243 27.60570427820866 26.77508131098324 20.760570427820866 135-139 25.95946960220165 26.810107580685518 27.695771828871653 19.534650988241182 140-144 26.20917321062372 26.324213474716153 27.21952683439204 20.247086480268095 145-149 26.69001751313485 27.435576682511886 26.21966474856142 19.654741055791845 150-151 28.283712784588445 27.282962221666253 25.569176882662 18.864148111083313 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.5 10 0.5 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.5 19 1.0 20 0.0 21 2.0 22 2.0 23 0.0 24 0.5 25 2.5 26 6.0 27 7.5 28 8.0 29 10.0 30 17.0 31 19.0 32 18.5 33 26.5 34 35.5 35 51.0 36 74.0 37 98.5 38 133.0 39 162.5 40 194.0 41 219.0 42 254.5 43 278.5 44 270.0 45 283.5 46 291.5 47 254.0 48 233.5 49 224.0 50 164.5 51 129.5 52 106.0 53 87.5 54 83.5 55 64.5 56 56.5 57 38.5 58 16.0 59 16.0 60 12.5 61 6.5 62 6.5 63 4.0 64 5.5 65 4.0 66 1.0 67 1.5 68 1.0 69 1.0 70 1.0 71 0.5 72 1.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 1.0 80 1.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.034999999999999996 25-29 0.075 30-34 0.055 35-39 0.034999999999999996 40-44 0.055 45-49 0.045 50-54 0.034999999999999996 55-59 0.075 60-64 0.034999999999999996 65-69 0.015 70-74 0.075 75-79 0.06 80-84 0.025 85-89 0.06999999999999999 90-94 0.04 95-99 0.03 100-104 0.075 105-109 0.075 110-114 0.034999999999999996 115-119 0.075 120-124 0.075 125-129 0.065 130-134 0.075 135-139 0.075 140-144 0.034999999999999996 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.65 #Duplication Level Percentage of deduplicated Percentage of total 1 69.66001478196601 47.125 2 19.17960088691796 25.95 3 7.354028085735403 14.924999999999999 4 2.2172949002217295 6.0 5 1.0347376201034737 3.5000000000000004 6 0.3325942350332594 1.35 7 0.11086474501108648 0.525 8 0.07390983000739099 0.4 9 0.03695491500369549 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCCATCCCAGTAGTGAGGATTTTGATAGTGGAGTGGATGATCTTGAAA 9 0.22499999999999998 No Hit GTTTGTCTGAAGAATCAGTGTTGGTAAATTCGTTAACGCCACTTTGCGGT 8 0.2 No Hit ATACAAACACTCGCACCATCCGATGATGTTCGTATGAGCAGTGTCATTGG 8 0.2 No Hit ATTGATTGGACACAATAAAACTGTGAGTTATGTGAAGTTTGTAGATACAA 7 0.17500000000000002 No Hit AAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATG 7 0.17500000000000002 No Hit TGCCTTCTTATTTCCGGGGCCCTGAAATTCCTTCAACGGTCTTCACTCTT 7 0.17500000000000002 No Hit TGATTAAATGGGCGCAGGTTTATTGCCATTGCACGTCAGTCTGCTGTTGC 6 0.15 No Hit CAGGTACCAGATATAATTGACATATGCATATGTTAGGGATGCTTGACCTT 6 0.15 No Hit GATCTTCTAGGGAAACTTAATTGGTCTGATCATCTTCCTTGGCTTGCAAA 6 0.15 No Hit GGCAGCTAATGTTGATGATCCTCATGTCAGCACATTGCGTTTAATTACGG 6 0.15 No Hit AGAATACAGATCCAGGTTGATTTTCTTTATCCTCTGTTTCTTAGCTTTTT 6 0.15 No Hit ATTGGCGCTCGGAGCCTCTGGCATATTCATTGGCCGACCAGTGGTCTTTT 6 0.15 No Hit GGTCAAAGTTTGCTACCCTGCCAAGACTAAGGTTTTAGCAGAACATGCCA 6 0.15 No Hit AGGCAGTCAAATGGCCACTACTGCTTCTCCAATGGCCAGCCAGCTCAAAA 6 0.15 No Hit AGGGAAGCCAGCTGGAGTCCTAACAGGACACTTAGAAGGCATAACATTTA 6 0.15 No Hit CATGTTGATTTCACACTTGAAGTGGAACGAGCTCTCAGGGTTTTGGACAG 5 0.125 No Hit CCCACGCAGCTCTTGCCTCTTCAAGAATCCCTGCCAATACAAGACTTCCC 5 0.125 No Hit CAGGATTAATAAACTGAAAAACCATAACCAGATTCACAGACTTCCTCAAT 5 0.125 No Hit CCTAACTCTTCATAACAATTTTCTTGATTGATCAGAGCTTTCATCATTAT 5 0.125 No Hit GAAAAGATAACATGGCCTGCTGAGATGGGCTCTCCTCCTCCCCTGTACTT 5 0.125 No Hit GATTAATAACATTCATCATGACATAGGAACCCATGTCATTCATCATCTCT 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CGTTACTGTTCAAGTGAATTAAATTTCACTTGAACAGTGCAAACACAAAG 5 0.125 No Hit TACAAGAAAGGCATGAAAATTTGCAATAAATGCTGCCAAAGTTTCCAATA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit CTTTACCACAACTGTGAGACTTAATTGGAAACAATGGCTTATATGTGTTG 5 0.125 No Hit TGTAAATCACATTACGTGAAACCCTTACCTGAAGTGTATACCCTCAAACA 5 0.125 No Hit CACACTTTAATTATTTATGATGATCTTTCCAAACAAGCGCAAGCTTATCG 5 0.125 No Hit CCTTAACTCATAAAAAAAGATAGACCGAGTGTTCGAGTCGTTCCAATCCC 5 0.125 No Hit AGATGAGGATGTCGCTTGGGAAGATTTTGCTGCAGGACCCAGATTTGTTG 5 0.125 No Hit GAAAGAGAAAGCTGATCTTGCAATTCAGAATCTCAGATGCCTTCCTCAGG 5 0.125 No Hit AAGAATGGTAGGTTGGCTATGTTCTCAATGTTCGGATTCTTTGTCCAGGC 5 0.125 No Hit ATACAATGTCAGAGTGGATGAGGTAAAATCAAAATTAAAGGGTCATCAGA 5 0.125 No Hit GGGAGATGCTGTCCCATTGAAGCTGAACTCCAACTTTCCATCACATCATT 5 0.125 No Hit TGGCAATGGCTACTCAAGCCTCTCTCTTCACCCCCACGACCCTCTCCACC 5 0.125 No Hit GTCAGCTACTGCACAGACAACAAGAAGTCCAAAGCCAAAGAAGAAAGGTA 5 0.125 No Hit GGGCCCTGGTGCTGAACAAGGAGGAATACTTCAGACATCATCATGCAAAC 5 0.125 No Hit GTATAGCCGATCTAATAAAAGAGCTGTTGCAGAGGCTGGTGGTGTTCAAG 5 0.125 No Hit AGCCATATGATTCTGTTATTGCAATAGCTTTTTAACAGCAAATCTGACTG 5 0.125 No Hit GCCAATGGATGAAGAAGAGAAGGTTGTGCTCGATTATTCATCTGATCCGT 5 0.125 No Hit GCTTACCATTGATAACCAAACCTCTAAGAAGAAGAAGCTCCTCTATAGGT 5 0.125 No Hit AAACCAGATCCTTTTGCTGAGGATAAACCAAGAATCTTTAAAAGGTTATC 5 0.125 No Hit GTGGAGAGTTTGATGCCCTCTACATTTGAGCGAAATGTTTATAGCTTGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.3 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.4 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.7375 0.0 0.0 0.0 0.0 94-95 0.9874999999999999 0.0 0.0 0.0 0.0 96-97 1.1 0.0 0.0 0.0 0.0 98-99 1.225 0.0 0.0 0.0 0.0 100-101 1.5125000000000002 0.0 0.0 0.0 0.0 102-103 1.7 0.0 0.0 0.0 0.0 104-105 1.9874999999999998 0.0 0.0 0.0 0.0 106-107 2.425 0.0 0.0 0.0 0.0 108-109 2.8875 0.0 0.0 0.0 0.0 110-111 3.325 0.0 0.0 0.0 0.0 112-113 3.6625 0.0 0.0 0.0 0.0 114-115 4.0 0.0 0.0 0.0 0.0 116-117 4.2875 0.0 0.0 0.0 0.0 118-119 4.5875 0.0 0.0 0.0 0.0 120-121 5.0625 0.0 0.0 0.0 0.0 122-123 5.725 0.0 0.0 0.0 0.0 124-125 6.2625 0.0 0.0 0.0 0.0 126-127 6.6375 0.0 0.0 0.0 0.0 128-129 6.9375 0.0 0.0 0.0 0.0 130-131 7.4125 0.0 0.0 0.0 0.0 132-133 7.825 0.0 0.0 0.0 0.0 134-135 8.4625 0.0 0.0 0.0 0.0 136-137 9.024999999999999 0.0 0.0 0.0 0.0 138-139 9.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTAAT 10 0.006830828 145.0 4 TAATGTT 10 0.006830828 145.0 7 GCTAATG 10 0.006830828 145.0 5 AATGTTG 10 0.006830828 145.0 8 >>END_MODULE Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848265 spots for SRR13695411.sra Written 848265 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra Read 848260 spots for SRR13695411.sra Written 848260 spots for SRR13695411.sra SRR ids: ['SRR13695411.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0e1id32k SRR13695411.sra spots: 16965205 blocks: [[1, 848260], [848261, 1696520], [1696521, 2544780], [2544781, 3393040], [3393041, 4241300], [4241301, 5089560], [5089561, 5937820], [5937821, 6786080], [6786081, 7634340], [7634341, 8482600], [8482601, 9330860], [9330861, 10179120], [10179121, 11027380], [11027381, 11875640], [11875641, 12723900], [12723901, 13572160], [13572161, 14420420], [14420421, 15268680], [15268681, 16116940], [16116941, 16965205]] SRR13695411 file size 5743818 SRR13695411 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695411 SRR13695411_1.fastq SRR13695411_2.fastq Input file: SRR13695411_1.fastq Paired file: SRR13695411_2.fastq trimmed: SRR13695411-trimmed-pair1.fastq, SRR13695411-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:19:04 2025 >> started Wed Feb 12 01:19:24 2025 >> done (19.953s) 16965205 read pairs processed; of these: 95 ( 0.00%) short read pairs filtered out after trimming by size control 2783 ( 0.02%) empty read pairs filtered out after trimming by size control 16962327 (99.98%) read pairs available; of these: 2474516 (14.59%) trimmed read pairs available after processing 14487811 (85.41%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 3 0.00% 20 5 0.00% 21 1 0.00% 22 1 0.00% 23 0 0.00% 24 0 0.00% 25 1 0.00% 26 3 0.00% 27 3 0.00% 28 4 0.00% 29 4 0.00% 30 4 0.00% 31 3 0.00% 32 4 0.00% 33 6 0.00% 34 5 0.00% 35 8 0.00% 36 6 0.00% 37 11 0.00% 38 9 0.00% 39 14 0.00% 40 13 0.00% 41 11 0.00% 42 12 0.00% 43 13 0.00% 44 23 0.00% 45 23 0.00% 46 22 0.00% 47 30 0.00% 48 42 0.00% 49 39 0.00% 50 77 0.00% 51 77 0.00% 52 86 0.00% 53 97 0.00% 54 102 0.00% 55 115 0.00% 56 120 0.00% 57 185 0.00% 58 172 0.00% 59 204 0.00% 60 284 0.00% 61 267 0.00% 62 340 0.00% 63 357 0.00% 64 407 0.00% 65 467 0.00% 66 592 0.00% 67 634 0.00% 68 778 0.00% 69 861 0.01% 70 961 0.01% 71 1214 0.01% 72 1402 0.01% 73 1581 0.01% 74 1837 0.01% 75 2026 0.01% 76 2221 0.01% 77 2532 0.01% 78 2728 0.02% 79 3214 0.02% 80 3512 0.02% 81 4081 0.02% 82 4596 0.03% 83 5197 0.03% 84 5847 0.03% 85 6216 0.04% 86 6951 0.04% 87 7541 0.04% 88 8071 0.05% 89 8628 0.05% 90 9753 0.06% 91 10230 0.06% 92 11182 0.07% 93 12473 0.07% 94 13294 0.08% 95 14148 0.08% 96 15492 0.09% 97 16386 0.10% 98 16792 0.10% 99 17794 0.10% 100 18993 0.11% 101 19331 0.11% 102 20991 0.12% 103 21677 0.13% 104 22810 0.13% 105 24527 0.14% 106 25222 0.15% 107 26777 0.16% 108 26861 0.16% 109 28356 0.17% 110 28851 0.17% 111 29766 0.18% 112 30973 0.18% 113 32119 0.19% 114 33413 0.20% 115 34630 0.20% 116 35395 0.21% 117 37668 0.22% 118 38443 0.23% 119 38479 0.23% 120 40172 0.24% 121 41521 0.24% 122 41470 0.24% 123 42956 0.25% 124 44473 0.26% 125 44976 0.27% 126 46367 0.27% 127 47095 0.28% 128 48705 0.29% 129 49499 0.29% 130 51139 0.30% 131 50676 0.30% 132 51533 0.30% 133 52556 0.31% 134 53131 0.31% 135 53696 0.32% 136 54871 0.32% 137 56063 0.33% 138 57338 0.34% 139 59156 0.35% 140 58591 0.35% 141 60134 0.35% 142 59625 0.35% 143 60288 0.36% 144 61962 0.37% 145 62146 0.37% 146 62606 0.37% 147 64641 0.38% 148 65012 0.38% 149 65842 0.39% 150 66544 0.39% 151 14487811 85.41% 16962327 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=32 prefix-density=0.51 prefix-fanout=2.0 sequence=GTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=35 fanout-score=40.95 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=7.8 sequence=AATGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTC criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=2.13 fanout-score-rank=31 prefix-density=0.44 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.15 sequence-density-rank=19 fanout-score=23.84 fanout-score-rank=1 prefix-density=0.73 prefix-fanout=5.0 sequence=AATGGCAGCCTCAGT SRR13695411 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:20:08 Started mapping on | Feb 12 01:20:08 Finished on | Feb 12 01:22:09 Mapping speed, Million of reads per hour | 504.66 Number of input reads | 16962327 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 16061191 Uniquely mapped reads % | 94.69% Average mapped length | 293.36 Number of splices: Total | 15641525 Number of splices: Annotated (sjdb) | 15311509 Number of splices: GT/AG | 15316799 Number of splices: GC/AG | 262911 Number of splices: AT/AC | 9680 Number of splices: Non-canonical | 52135 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 359515 % of reads mapped to multiple loci | 2.12% Number of reads mapped to too many loci | 23479 % of reads mapped to too many loci | 0.14% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.97% % of reads unmapped: other | 0.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 541812 541812 541812 N_multimapping 359515 359515 359515 N_noFeature 492698 15650628 715093 N_ambiguous 285736 1372 96767 UnstrandedReadsAssigned:15282757 PositiveStrandReadsAssigned:409191 NegativeStrandReadsAssigned:15249331 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695411 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695411-trimmed-pair1.fastq SRR13695411-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,962,327 reads, 15,277,938 reads pseudoaligned [quant] estimated average fragment length: 236.635 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 992 rounds 52401 SRR13695411.ke.tsv 34699 SRR13695411.se.tsv 87100 total ==> SRR13695411.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1782.36 482 14.8535 Potri.005G024800.1.v4.1 1035 799.365 391 26.8664 Potri.004G059700.1.v4.1 961 725.428 0 0 Potri.007G009000.2.v4.1 1416 1180.36 0 0 Potri.003G141000.2.v4.1 2943 2707.36 775 15.7229 Potri.016G087400.1.v4.1 270 89.4232 806 495.066 Potri.015G069301.1.v4.1 564 335.641 0 0 Potri.010G195200.1.v4.1 1773 1537.36 64 2.28655 Potri.012G127500.1.v4.1 977 741.397 113 8.37155 ==> SRR13695411.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 112 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 324 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695411 completed mapping pipeline successfully