Starting /dee2/code/volunteer_pipeline.sh SRR13695413 current disk space = 3051171344384 free memory = 890071036 SRR13695413 SRAfilesize 7cf7109851b8452a59d2d9e45b581875 SRR13695413.sra SRR13695413.sra file validated SRR13695413 is paired end SRR13695413 is conventional basespace SRR13695413 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695413_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.668 37.0 37.0 37.0 37.0 37.0 2 36.33475 37.0 37.0 37.0 37.0 37.0 3 36.6075 37.0 37.0 37.0 37.0 37.0 4 36.6345 37.0 37.0 37.0 37.0 37.0 5 36.615 37.0 37.0 37.0 37.0 37.0 6 36.609 37.0 37.0 37.0 37.0 37.0 7 36.471 37.0 37.0 37.0 37.0 37.0 8 36.682 37.0 37.0 37.0 37.0 37.0 9 36.577 37.0 37.0 37.0 37.0 37.0 10-14 36.6104 37.0 37.0 37.0 37.0 37.0 15-19 36.560700000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.531099999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.45399999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.4698 37.0 37.0 37.0 37.0 37.0 35-39 36.4505 37.0 37.0 37.0 37.0 37.0 40-44 36.41709999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.4178 37.0 37.0 37.0 37.0 37.0 50-54 36.43920000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.4116 37.0 37.0 37.0 37.0 37.0 60-64 36.3365 37.0 37.0 37.0 37.0 37.0 65-69 36.379200000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.3427 37.0 37.0 37.0 37.0 37.0 75-79 36.3121 37.0 37.0 37.0 37.0 37.0 80-84 36.2316 37.0 37.0 37.0 37.0 37.0 85-89 36.282 37.0 37.0 37.0 37.0 37.0 90-94 36.2632 37.0 37.0 37.0 37.0 37.0 95-99 36.2004 37.0 37.0 37.0 37.0 37.0 100-104 36.1708 37.0 37.0 37.0 37.0 37.0 105-109 36.1485 37.0 37.0 37.0 37.0 37.0 110-114 36.1649 37.0 37.0 37.0 37.0 37.0 115-119 36.106700000000004 37.0 37.0 37.0 37.0 37.0 120-124 36.1259 37.0 37.0 37.0 37.0 37.0 125-129 36.1125 37.0 37.0 37.0 37.0 37.0 130-134 36.0781 37.0 37.0 37.0 37.0 37.0 135-139 35.985 37.0 37.0 37.0 37.0 37.0 140-144 35.8839 37.0 37.0 37.0 37.0 37.0 145-149 35.6907 37.0 37.0 37.0 37.0 37.0 150-151 35.43925 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 0.0 23 0.0 24 1.0 25 3.0 26 9.0 27 7.0 28 13.0 29 11.0 30 26.0 31 32.0 32 34.0 33 57.0 34 134.0 35 323.0 36 2970.0 37 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.425000000000004 13.5 8.25 40.825 2 21.538074893189243 12.8424227192762 35.86328223171651 29.75622015581805 3 18.85 14.45 27.075 39.625 4 23.0 22.3 24.8 29.9 5 23.95 30.425 24.25 21.375 6 22.525000000000002 32.125 23.925 21.425 7 15.75 28.675 40.9 14.674999999999999 8 17.4 27.325 32.925 22.35 9 18.5 22.8 35.225 23.474999999999998 10-14 19.41 29.25 28.705000000000002 22.634999999999998 15-19 20.27 28.294999999999998 28.055000000000003 23.380000000000003 20-24 20.69 27.82 27.97 23.52 25-29 20.06 28.310000000000002 27.950000000000003 23.68 30-34 20.405 28.549999999999997 28.29 22.755 35-39 20.54 28.93 27.465 23.064999999999998 40-44 20.435 28.685 27.27 23.61 45-49 20.395 28.225 27.46 23.919999999999998 50-54 20.7 28.42 27.229999999999997 23.65 55-59 20.07 28.315 28.285 23.330000000000002 60-64 20.315 28.389999999999997 27.445000000000004 23.849999999999998 65-69 20.395 29.085 26.63 23.89 70-74 20.115 28.599999999999998 27.355 23.93 75-79 20.965 27.875 27.16 24.0 80-84 20.565 28.249999999999996 27.935 23.25 85-89 20.495 27.83 27.805000000000003 23.87 90-94 20.445 28.79 27.515 23.25 95-99 20.625 27.29 28.73 23.355 100-104 20.195 29.39 26.6 23.815 105-109 20.345 28.33 27.445000000000004 23.880000000000003 110-114 21.245 27.355 27.944999999999997 23.455000000000002 115-119 20.31 27.965 27.47 24.255 120-124 21.355 27.93 27.400000000000002 23.315 125-129 20.78 28.46 26.93 23.830000000000002 130-134 21.115000000000002 27.810000000000002 27.215 23.86 135-139 21.81 27.744999999999997 26.52 23.925 140-144 21.475 28.21 26.405 23.91 145-149 20.985 28.255000000000003 26.47 24.29 150-151 21.775 26.6125 26.6625 24.95 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 0.5 24 2.5 25 3.5 26 4.5 27 9.5 28 9.5 29 11.5 30 10.5 31 20.5 32 32.5 33 30.5 34 56.0 35 75.5 36 81.5 37 93.0 38 116.5 39 151.5 40 188.5 41 208.5 42 222.0 43 267.5 44 297.5 45 271.5 46 250.5 47 265.0 48 247.0 49 210.5 50 187.5 51 155.5 52 132.5 53 106.5 54 69.0 55 47.5 56 42.5 57 36.5 58 23.0 59 17.0 60 13.0 61 9.5 62 7.0 63 3.0 64 0.5 65 0.5 66 0.5 67 2.0 68 2.0 69 0.0 70 0.5 71 2.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.925 #Duplication Level Percentage of deduplicated Percentage of total 1 68.63860447478196 45.25 2 19.226393629124004 25.35 3 7.318923018581722 14.475 4 2.7682973075464545 7.3 5 1.3651877133105803 4.5 6 0.37921880925293894 1.5 7 0.15168752370117558 0.7000000000000001 8 0.11376564277588168 0.6 9 0.0 0.0 >10 0.037921880925293895 0.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGCTAGAAGATAAGAAGATGGCTTCTCTTCCCATCGCCTTCGCTGTTCCT 13 0.325 No Hit AGGCACTCCAAATTGAAAAGCAAGGTTTGCTCCAAGTCCCAAATTTGTAC 8 0.2 No Hit ACGTAATCAAGTTGGCGAGCGCCGTGATTCGACACTATAATTCCTGCTGC 8 0.2 No Hit CCCCAGTTGGCATTTCAAATATTTGTTCCTTGGGGGAGTCCCATGTGATC 8 0.2 No Hit CTCTGGTCTCGTACCATCGATGGCATACCCATCCGACCATTCATTATAGA 7 0.17500000000000002 No Hit CATATTTGTCGACATATTGGTACACCCCCTTTCCCCTGGGCTTCCTTCCA 7 0.17500000000000002 No Hit CCCTTATTCAGTGTAGTGGCATTTTCATTCTAAGCCATGAAGCGCTTCTT 7 0.17500000000000002 No Hit CAGGTGTGTTCCTAAGAGCTGCTAGCATAGTAGATGCCAGTGAACGTCTT 7 0.17500000000000002 No Hit CCACTGATGATAATAACAAATACTGTTGTCCAGAAGCTTCCATAAAACCA 6 0.15 No Hit CTCCAGTATACTTGCCAAGAGTTGCCTCTGAATTTCCCTTGCACCTAACC 6 0.15 No Hit ATTTGAGGGATTTTACACAGCACTTTCTTGATCTTCTTGCGGCATTTCTT 6 0.15 No Hit GTTGTCCAGTTGAGATGGCCTAATTGAGGTTGCTGATAAGCACCCACTTG 6 0.15 No Hit CCACACCATTCTCTCTCCCGGATACCGCTGCTAGCACAAAGAGCGCAACT 6 0.15 No Hit CTCCCCTCGTAGCTAATGACCGAAAAACAGGAAATACAACAATTGGAGTT 6 0.15 No Hit GGTCAAAGTCAAACTTGTCTTCATCAGCTGGATCAATTATTTGAATGAAA 6 0.15 No Hit TCTCGAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTT 6 0.15 No Hit CGTCTCTAGGTCAATGCCACGTGCTTGAAAGTAGAAGAGTTGGGTTTCTT 6 0.15 No Hit CCCATGAGGACAACTTGGAATCCAAGGACAGCAAGGATGCTTTGGGCATG 6 0.15 No Hit GCCCCCTCTTCCCTTTGGGTCCAAGAAAGATGAACCTCGGTAGGATGGTA 5 0.125 No Hit TCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTGCATCCAAACATGGG 5 0.125 No Hit GACTGGGACAAGTGATTTGCAGCTCAGACTTTGCCATTGGCACGGGCTCC 5 0.125 No Hit CCCTTCTCTAACTTCTTCATTGACACAGAAAGCCTCCCAAGCTCCCCAAC 5 0.125 No Hit GCCTGTTTTGATCAATTGCAACAAACAAAATCCCCTTATTCCTAGCCAAA 5 0.125 No Hit GACCCACTTCCTCACCTCATCGTCATATACTCGGGCCCTGAGAGCACCAA 5 0.125 No Hit CGGGTATTCAAGCATTGATAACATATCTGAATTGGCACTTTGTATCACCA 5 0.125 No Hit GTCTTTTTGGCTTTCTTCTGAGCTAAAACCAACATAGGTATTCTTTTGTC 5 0.125 No Hit GGGTTTGATCTCCATAGGCTGGAACCTGGGTACCCTTTGATTATAGGTGG 5 0.125 No Hit TCTTGACTGTTGCAGTGTTTGAGCAAATGCCTGATACTTCCGGATGTCAG 5 0.125 No Hit CACCACAGATATTGCGACCCCTGCAGATTTTGTAATAGCCATTCTCTCCC 5 0.125 No Hit CACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACAC 5 0.125 No Hit CATCACCCGATGGAGCATGTGAGATTTGGACGCGGGAGTCAGCTGCAAGC 5 0.125 No Hit GTTCCTCTGAACTTTGGTTTTCTTTTGGATTACCCCAGAGATCGCTCCAG 5 0.125 No Hit ATATCATCATCTTCATCACTCCAATCATCTTCATAATCATCATCTTCATC 5 0.125 No Hit GGGCAATTGACGTCGTGACTGCATCCAGTATAAGCTGTGTCTTCCTGCTT 5 0.125 No Hit GTCCCTTGCATTTTTCCTCCACGCTGGGCTTTCCCTCCATGGAACAGCTC 5 0.125 No Hit CGCTTGGCGATGTCTTCGAGCTTAGAGATGATGGTGGAGGCAGGCTTGTT 5 0.125 No Hit GCCAGTTGTACGGAGGTAGTTATCCAATTCCACACCCTTGTCAACTCCAG 5 0.125 No Hit ATTTACAACAGCGACCAGTGGAGGAGAACGCATGGCAGGATGACCTGCAC 5 0.125 No Hit GTCAGAAGCCACAAATAAGGTGGAAGTGGACTTGTCGAAGACAATGAATG 5 0.125 No Hit CCGGCATTGTTCTTGAAGACAATCTTTTCACCAGCGGGTACAGAGAATTC 5 0.125 No Hit CAGGTTTCTTAAAAATCAAATCTGTGGTAGCAAATGGAACCATGTACTCT 5 0.125 No Hit GGCTTGCTTTCTCTGCCTCCTTTTCCCAGTCGCATCGAATGGTAATGATG 5 0.125 No Hit GTCCAAAACCATGCATTCATCAGCGTTTTCTACAAGCTGGTGGACAGAAA 5 0.125 No Hit GTCATCATCATCATCTAGTCTCAGTAACCTCCTTGTATGTAAGCATTCAG 5 0.125 No Hit AGCAGGGTATCCACTATCAGCAGGCATTTCTGCCAGCCGTCCAGAAATTT 5 0.125 No Hit CTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGA 5 0.125 No Hit CCTGATTCTACCAAATCTTTCTGGGTCCGGGCGGGCTGGCTGGCTTCGTT 5 0.125 No Hit CTCGATTCATTATTCGGATTCCAAAACCTAAACTAATCACTAATCCAAGA 5 0.125 No Hit ATGGGCGGATGCTTGACTGTAAGTTCAAATCCAAGCCTAAGAGATAAACT 5 0.125 No Hit CTGGATCTTGAAGAAGTGGACCTGTTCGTAATGATAAAGGAGGCTAAAGT 5 0.125 No Hit CCCCAATTCGCTCCCATCCTCCATAAAGAACCCATTGTGAAACCAACTAA 5 0.125 No Hit GCCATCAAACCTTTCTTGATGTCCGCCTGTAATTCCCTGATTGGAATTGC 5 0.125 No Hit GGATGCGACCCCAATCAGTTTGAGAAACCCCAGGTCAGGAGTTCGGGTAA 5 0.125 No Hit TCCCCGGCCTGCAAACTCTTTGCTAACATCCTAGCCATGACTAACTTTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0125 0.0 0.0 0.0 0.0 76-77 0.11249999999999999 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.36250000000000004 0.0 0.0 0.0 0.0 86-87 0.48750000000000004 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.825 0.0 0.0 0.0 0.0 92-93 1.175 0.0 0.0 0.0 0.0 94-95 1.3375 0.0 0.0 0.0 0.0 96-97 1.475 0.0 0.0 0.0 0.0 98-99 1.6625 0.0 0.0 0.0 0.0 100-101 1.85 0.0 0.0 0.0 0.0 102-103 2.1375 0.0 0.0 0.0 0.0 104-105 2.4375 0.0 0.0 0.0 0.0 106-107 2.85 0.0 0.0 0.0 0.0 108-109 3.05 0.0 0.0 0.0 0.0 110-111 3.3625 0.0 0.0 0.0 0.0 112-113 3.825 0.0 0.0 0.0 0.0 114-115 4.2875 0.0 0.0 0.0 0.0 116-117 4.575 0.0 0.0 0.0 0.0 118-119 4.9125 0.0 0.0 0.0 0.0 120-121 5.35 0.0 0.0 0.0 0.0 122-123 5.875 0.0 0.0 0.0 0.0 124-125 6.225 0.0 0.0 0.0 0.0 126-127 6.737500000000001 0.0 0.0 0.0 0.0 128-129 7.2875 0.0 0.0 0.0 0.0 130-131 8.0125 0.0 0.0 0.0 0.0 132-133 8.8 0.0 0.0 0.0 0.0 134-135 9.7625 0.0 0.0 0.0 0.0 136-137 10.65 0.0 0.0 0.0 0.0 138-139 11.4875 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAGTA 10 0.006830828 145.0 2 ACGAAGA 10 0.006830828 145.0 3 >>END_MODULE SRR13695413 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695413_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.23675 37.0 37.0 37.0 37.0 37.0 2 36.292 37.0 37.0 37.0 37.0 37.0 3 36.177 37.0 37.0 37.0 37.0 37.0 4 36.377 37.0 37.0 37.0 37.0 37.0 5 36.312 37.0 37.0 37.0 37.0 37.0 6 36.262 37.0 37.0 37.0 37.0 37.0 7 36.34 37.0 37.0 37.0 37.0 37.0 8 36.3635 37.0 37.0 37.0 37.0 37.0 9 36.279 37.0 37.0 37.0 37.0 37.0 10-14 36.31270000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.330799999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.25085 37.0 37.0 37.0 37.0 37.0 25-29 36.201049999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.16105 37.0 37.0 37.0 37.0 37.0 35-39 36.18155 37.0 37.0 37.0 37.0 37.0 40-44 36.15645 37.0 37.0 37.0 37.0 37.0 45-49 36.14615 37.0 37.0 37.0 37.0 37.0 50-54 36.08855 37.0 37.0 37.0 37.0 37.0 55-59 36.09375 37.0 37.0 37.0 37.0 37.0 60-64 36.13685 37.0 37.0 37.0 37.0 37.0 65-69 35.99255000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.03345 37.0 37.0 37.0 37.0 37.0 75-79 36.051649999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.04295 37.0 37.0 37.0 37.0 37.0 85-89 35.87365 37.0 37.0 37.0 37.0 37.0 90-94 35.90525 37.0 37.0 37.0 37.0 37.0 95-99 35.888549999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.903949999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.887649999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.77315 37.0 37.0 37.0 37.0 37.0 115-119 35.74115 37.0 37.0 37.0 37.0 37.0 120-124 35.69835 37.0 37.0 37.0 37.0 37.0 125-129 35.732350000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.56595 37.0 37.0 37.0 37.0 37.0 135-139 35.560199999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.43194999999999 37.0 37.0 37.0 34.6 37.0 145-149 35.3667 37.0 37.0 37.0 37.0 37.0 150-151 35.07325 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 3.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 1.0 15 2.0 16 0.0 17 2.0 18 0.0 19 1.0 20 0.0 21 2.0 22 0.0 23 4.0 24 5.0 25 5.0 26 5.0 27 13.0 28 11.0 29 14.0 30 28.0 31 30.0 32 55.0 33 99.0 34 183.0 35 554.0 36 2744.0 37 237.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.8306148055207 23.663739021329988 12.396486825595986 29.109159347553327 2 27.800000000000004 23.225 33.050000000000004 15.925 3 21.55 28.299999999999997 28.725 21.425 4 22.900000000000002 34.425 23.05 19.625 5 25.674999999999997 37.35 20.424999999999997 16.55 6 20.7 38.775 23.400000000000002 17.125 7 19.925 24.775 37.225 18.075 8 20.1 25.900000000000002 28.725 25.275 9 21.925 25.825 29.799999999999997 22.45 10-14 23.52 29.5 26.19 20.79 15-19 23.169999999999998 28.73 26.58 21.52 20-24 23.332499374530897 28.211158368776584 27.410557918438826 21.04578433825369 25-29 22.626970227670753 28.661496122091567 28.071053289967473 20.640480360270203 30-34 23.007255441581187 28.386289717287966 28.10607955966975 20.500375281461096 35-39 22.952214160620464 29.29196897673255 26.89016762571929 20.8656492369277 40-44 22.722041531148363 28.83162371778834 27.380535401551164 21.065799349512133 45-49 23.29246935201401 27.990993244933698 27.715786840130097 21.000750562922192 50-54 22.431823867900928 27.850888166124594 28.126094570928196 21.591193395046286 55-59 22.61696272204153 27.290467850888167 28.481361020765572 21.611208406304726 60-64 22.476857643232425 27.825869402051538 27.77082812109082 21.92644483362522 65-69 23.6977733299975 27.46559919939955 27.295471603702776 21.541155866900176 70-74 23.86289717287966 27.070302727045288 27.675756817613212 21.391043282461847 75-79 22.61195896922692 27.690768076057044 28.256192144108084 21.441080810607957 80-84 23.262446835126344 27.995996997748314 27.075306479859897 21.66624968726545 85-89 22.066549912434326 28.386289717287966 27.4906179634726 22.056542406805104 90-94 24.003002251688766 27.72579434575932 26.830122591943955 21.441080810607957 95-99 23.802852139104328 28.04103077307981 27.135351513635225 21.020765574180636 100-104 23.362521891418563 27.4906179634726 27.980985739304476 21.165874405804352 105-109 24.11808856642482 28.186139604703524 27.615711783837877 20.080060045033775 110-114 23.70778083562672 28.45133850387791 27.05028771578684 20.79059294470853 115-119 23.582687015261445 28.91668751563673 26.659994996247182 20.84063047285464 120-124 24.0180135101326 28.056042031523642 27.31548661496122 20.610457843382537 125-129 25.12884663497623 27.865899424568426 27.05028771578684 19.954966224668503 130-134 25.486663664114495 27.44833108141921 26.592603713156183 20.472401541310113 135-139 26.381104883907124 27.256805444355486 26.901521216973578 19.46056845476381 140-144 26.720040030022517 28.236177132849637 25.749311983987994 19.294470853139856 145-149 27.827261809447556 27.361889511609288 25.66052842273819 19.150320256204964 150-151 28.015515515515517 27.665165165165167 24.5995995995996 19.71971971971972 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 0.5 18 1.0 19 1.0 20 0.0 21 0.5 22 0.5 23 0.0 24 1.5 25 1.5 26 1.5 27 3.0 28 5.0 29 8.5 30 12.0 31 14.5 32 17.0 33 32.5 34 46.5 35 57.5 36 80.5 37 105.0 38 131.5 39 161.0 40 186.5 41 221.5 42 262.5 43 282.5 44 278.0 45 289.0 46 293.0 47 260.5 48 224.5 49 209.5 50 175.0 51 133.0 52 109.0 53 81.0 54 72.5 55 60.0 56 40.0 57 30.0 58 22.5 59 25.0 60 22.5 61 13.5 62 11.5 63 4.0 64 0.0 65 0.0 66 0.0 67 1.0 68 2.5 69 1.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.075 25-29 0.075 30-34 0.075 35-39 0.075 40-44 0.075 45-49 0.075 50-54 0.075 55-59 0.075 60-64 0.075 65-69 0.075 70-74 0.075 75-79 0.075 80-84 0.075 85-89 0.075 90-94 0.075 95-99 0.075 100-104 0.075 105-109 0.075 110-114 0.075 115-119 0.075 120-124 0.075 125-129 0.075 130-134 0.08499999999999999 135-139 0.08 140-144 0.075 145-149 0.08 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.2 #Duplication Level Percentage of deduplicated Percentage of total 1 69.37311178247734 45.925 2 18.731117824773413 24.8 3 7.212990936555891 14.325 4 2.56797583081571 6.800000000000001 5 1.2084592145015105 4.0 6 0.49093655589123864 1.95 7 0.18882175226586104 0.8750000000000001 8 0.18882175226586104 1.0 9 0.0 0.0 >10 0.0377643504531722 0.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCATAGAACTGGTCTTCCTTTTGCAAACTCCCAATAATCCAGTCAAGAA 13 0.325 No Hit GTCAATTCACCGAATCAGAAATATCTGACTTTGTATCCACTAAAAACCTT 8 0.2 No Hit CTCAAGCCTCTCTCTTCACCCCCACGACCCTCTCCACCCTAAAATCAAGC 8 0.2 No Hit CCATCATCGTCACTGGTAACGATTTTTCAACATTGTATGCTCCTCTTATC 8 0.2 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 8 0.2 No Hit CTTTGAAGGTTTGGACCTTGGCAAGATGGACAAGGCCGATGACTCTGGAC 8 0.2 No Hit CTACATCCCATTCGTTGAAAGATTCTGCATCTTTCTGTCAGAAGTGTTTA 7 0.17500000000000002 No Hit GCCGTTGCCACCGTCAACCGCACCCCGGCACAAGCCAACATGGTGGCACC 7 0.17500000000000002 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 7 0.17500000000000002 No Hit TTCAGATTTTGGATTGGCAAAACTGGTTGGCAAAACAGGCGAGGGAGAAG 7 0.17500000000000002 No Hit CAGTAATGGCAGCCTCAGTTATGGCTTCACTGAACCTGAAACCATCTCCA 7 0.17500000000000002 No Hit AAGGCATTGAATGACCAGCATGTTCTTCTTGAAGGCACCCTCCTCAAGCC 6 0.15 No Hit GGATTCCTTTTTACGGCTGGGGACAACATCCTTTTTTTCAGTCATTTGGT 6 0.15 No Hit GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT 6 0.15 No Hit TGTCAACAGGCAACAATACGGTTCTCAATGGAGGAAATGGCCTGGTGGGA 6 0.15 No Hit TGTGAAATTCCACTGGAAACCAACATGTGGGGTCAAGTCTTTATTGGAAG 6 0.15 No Hit CACACAACACAAACAAGACCAGCAAAAACCAGGACAAAAAAGTTCAAGAA 6 0.15 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 6 0.15 No Hit CAACTTTCCACTTGTCTGTTGGTGATCAAACACAAGATTTACACAGTAGG 6 0.15 No Hit TTTGAAGAAAAAAAGAAACAACTTTACTGACAATTACTTGTTTGGTCAGA 6 0.15 No Hit TGATGAGATGTGATAAATCTTCATTTATACGGCTCTCTCTCGGATATCTC 6 0.15 No Hit TAAATCAACAACTGGACCACAGATAGTGTTCGTGAGAAGAAACAGCTGAA 6 0.15 No Hit GGTAATGGTTTGTACAATCATCAATGTTCATGGCAGAGTACGGGACAAGA 6 0.15 No Hit CTCGGATGAAGTACAAAGATGGAAACCTTTATGTTCCTAATCCACAGGCT 6 0.15 No Hit GATAAACGCATCAGGCACATTCCTGTGATTGATGATAAGGAAATGATTGG 5 0.125 No Hit GGGTGGTTATGATAAGGTTGTTGCAGAGAAGAAATGGAGGGAAGTGGGTT 5 0.125 No Hit AAGTTGTAGCATCCGTATCTAACTTCAGTGTTGTTTCAATCGATGAAGAT 5 0.125 No Hit TCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCA 5 0.125 No Hit AAAACCATTAATCCTCATAGGTGTTGACCTATAGTAATGTATTGTCAAGT 5 0.125 No Hit CAATGTACCAATCCAGGGGAATTTACATGACTGCAAAGGTTGCCTTTTGC 5 0.125 No Hit GGGGGAATAGTTGTCTATGACAACACACTATGGGGAGGGACAGTTGCTTT 5 0.125 No Hit GTAATCTCTTCGTTGAGATGGGGTGTTGCGGGCGGAAGAAAAACTATGGT 5 0.125 No Hit TGAAAGTGTAAGCAAGAATGCCCCACCTGAGTTCCAAAACACTAAGCTCA 5 0.125 No Hit TGGGAAAGTGGCAACTTTCCATTGCTCTTTTTTGGCTCATTTGACAATGG 5 0.125 No Hit GGAAAATGGCATCACCATTTACAGCGGCAACAACTCTTTCATGCACTTCT 5 0.125 No Hit CCCCTCTCTCCTTAAATTATAAATCCTCTGTCATTCTTGAAATCTTGCTA 5 0.125 No Hit GTGTATTACAATGAAGCAAGTGGTGGAAGATATGTGCCACGTGCTGTTCT 5 0.125 No Hit AAGGACATGGAGGAGGATAGAGAACATGTACCCTCTTCCCAGTGCAGGTC 5 0.125 No Hit AGAAAACAATGGGTGATCAGCAGAATGTGCCAGCCACAGTGGAACCTAAG 5 0.125 No Hit ATGCTTGGAGCACTAGGCTGCATCACCCCAGAGATCCTTGAGAAATGGGT 5 0.125 No Hit AAAAACCATGGCAACCCTTCGTTTCACTCCATCTCCTTCCTCCATCCTCA 5 0.125 No Hit ACCCAATATTGCATCCACAACACAATGAAGTAACACATCCCCATCAGAGT 5 0.125 No Hit AAATCTTCAATTAGTCCAGCAAGCAATTGAAAGTTGCCCGGTTGATTGCA 5 0.125 No Hit ACTCTAACTCTGATACTGTTGTGTATGTGGGTTGTGGAGAAAGAGGAAAT 5 0.125 No Hit CCAAAGCAAGAAGTGGCGAAGCCTTGCAACTTAAATGCTTTCGATATTAT 5 0.125 No Hit CATAAACCTAGGTTGCTACTATGCTATTGGAGTTCCTCTTGGATTCCTAA 5 0.125 No Hit GAAACTTTGCCTGAAACTTTTATGCTAAACCTGTCTCGGTTGAGGTCTGT 5 0.125 No Hit GTCAACTTTCTTGTTTCTTGATCAAATTGTTTGGCTTGGAAGAAGTGGCA 5 0.125 No Hit AAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATG 5 0.125 No Hit GATCTCATCGGCAGCCGTTGCCACCGTCAACCGCACCCCGGCACAAGCCA 5 0.125 No Hit CCTTTGTAGCAGTGCTCAGTCCTGACTCAAGCTTCTTCCAAATTGAAGAA 5 0.125 No Hit CTCATTTGGTAGAGAAGGACCGGCTATTGCTCATCAAAGAGAAGCTTCGT 5 0.125 No Hit AGAGGAGGCAAAAAGAGAACCCTGAGGCTATGGAGGAAGATGTTGAGGAT 5 0.125 No Hit ATAGCTACTTCTTGAAGATGGCAAGCGAGGCTGGAGAAGCAACCTCTGGA 5 0.125 No Hit AACTGATCTCTAGATTCGGCACATTGGAGAATTTGTTGAAATGTGTTGAT 5 0.125 No Hit CAGTAGCTAGTACAGGCTATTTACCCTTTAATCATGTGTTGTCAGAGAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.037500000000000006 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.3125 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.375 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.675 0.0 0.0 0.0 0.0 90-91 0.8500000000000001 0.0 0.0 0.0 0.0 92-93 1.2 0.0 0.0 0.0 0.0 94-95 1.3625 0.0 0.0 0.0 0.0 96-97 1.5 0.0 0.0 0.0 0.0 98-99 1.6875 0.0 0.0 0.0 0.0 100-101 1.875 0.0 0.0 0.0 0.0 102-103 2.1625 0.0 0.0 0.0 0.0 104-105 2.4625 0.0 0.0 0.0 0.0 106-107 2.875 0.0 0.0 0.0 0.0 108-109 3.075 0.0 0.0 0.0 0.0 110-111 3.3875 0.0 0.0 0.0 0.0 112-113 3.85 0.0 0.0 0.0 0.0 114-115 4.3125 0.0 0.0 0.0 0.0 116-117 4.6 0.0 0.0 0.0 0.0 118-119 4.975 0.0 0.0 0.0 0.0 120-121 5.425 0.0 0.0 0.0 0.0 122-123 5.9625 0.0 0.0 0.0 0.0 124-125 6.3 0.0 0.0 0.0 0.0 126-127 6.8125 0.0 0.0 0.0 0.0 128-129 7.362500000000001 0.0 0.0 0.0 0.0 130-131 8.0875 0.0 0.0 0.0 0.0 132-133 8.8625 0.0 0.0 0.0 0.0 134-135 9.837499999999999 0.0 0.0 0.0 0.0 136-137 10.725 0.0 0.0 0.0 0.0 138-139 11.5875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra Read 1104588 spots for SRR13695413.sra Written 1104588 spots for SRR13695413.sra Read 1104583 spots for SRR13695413.sra Written 1104583 spots for SRR13695413.sra SRR ids: ['SRR13695413.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_l34an94t SRR13695413.sra spots: 22091665 blocks: [[1, 1104583], [1104584, 2209166], [2209167, 3313749], [3313750, 4418332], [4418333, 5522915], [5522916, 6627498], [6627499, 7732081], [7732082, 8836664], [8836665, 9941247], [9941248, 11045830], [11045831, 12150413], [12150414, 13254996], [13254997, 14359579], [14359580, 15464162], [15464163, 16568745], [16568746, 17673328], [17673329, 18777911], [18777912, 19882494], [19882495, 20987077], [20987078, 22091665]] SRR13695413 file size 7486013 SRR13695413 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695413 SRR13695413_1.fastq SRR13695413_2.fastq Input file: SRR13695413_1.fastq Paired file: SRR13695413_2.fastq trimmed: SRR13695413-trimmed-pair1.fastq, SRR13695413-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:14:32 2025 >> started Wed Feb 12 01:14:57 2025 >> done (25.430s) 22091665 read pairs processed; of these: 163 ( 0.00%) short read pairs filtered out after trimming by size control 1339 ( 0.01%) empty read pairs filtered out after trimming by size control 22090163 (99.99%) read pairs available; of these: 3646285 (16.51%) trimmed read pairs available after processing 18443878 (83.49%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 1 0.00% 20 1 0.00% 21 0 0.00% 22 2 0.00% 23 1 0.00% 24 5 0.00% 25 1 0.00% 26 4 0.00% 27 2 0.00% 28 6 0.00% 29 3 0.00% 30 4 0.00% 31 4 0.00% 32 1 0.00% 33 6 0.00% 34 8 0.00% 35 14 0.00% 36 7 0.00% 37 12 0.00% 38 12 0.00% 39 14 0.00% 40 30 0.00% 41 17 0.00% 42 33 0.00% 43 34 0.00% 44 39 0.00% 45 29 0.00% 46 36 0.00% 47 60 0.00% 48 86 0.00% 49 68 0.00% 50 106 0.00% 51 96 0.00% 52 134 0.00% 53 192 0.00% 54 177 0.00% 55 179 0.00% 56 221 0.00% 57 264 0.00% 58 360 0.00% 59 366 0.00% 60 436 0.00% 61 575 0.00% 62 672 0.00% 63 814 0.00% 64 812 0.00% 65 992 0.00% 66 1180 0.01% 67 1347 0.01% 68 1430 0.01% 69 1682 0.01% 70 1978 0.01% 71 2310 0.01% 72 2753 0.01% 73 3229 0.01% 74 3570 0.02% 75 4129 0.02% 76 4443 0.02% 77 5071 0.02% 78 5669 0.03% 79 6265 0.03% 80 6859 0.03% 81 7903 0.04% 82 8760 0.04% 83 9886 0.04% 84 10930 0.05% 85 12189 0.06% 86 13209 0.06% 87 14171 0.06% 88 15055 0.07% 89 15947 0.07% 90 17101 0.08% 91 18301 0.08% 92 19437 0.09% 93 21493 0.10% 94 22752 0.10% 95 24331 0.11% 96 26254 0.12% 97 27403 0.12% 98 28526 0.13% 99 29900 0.14% 100 31074 0.14% 101 31954 0.14% 102 33061 0.15% 103 35115 0.16% 104 36574 0.17% 105 37903 0.17% 106 40389 0.18% 107 41485 0.19% 108 42700 0.19% 109 44088 0.20% 110 44473 0.20% 111 46237 0.21% 112 47678 0.22% 113 48606 0.22% 114 50581 0.23% 115 51601 0.23% 116 54150 0.25% 117 55567 0.25% 118 57227 0.26% 119 57459 0.26% 120 59374 0.27% 121 60451 0.27% 122 62181 0.28% 123 62979 0.29% 124 64061 0.29% 125 64961 0.29% 126 66877 0.30% 127 68569 0.31% 128 69685 0.32% 129 70449 0.32% 130 71885 0.33% 131 72113 0.33% 132 72842 0.33% 133 74252 0.34% 134 75284 0.34% 135 75734 0.34% 136 77196 0.35% 137 78114 0.35% 138 79256 0.36% 139 81993 0.37% 140 82435 0.37% 141 83330 0.38% 142 83903 0.38% 143 83483 0.38% 144 85305 0.39% 145 85581 0.39% 146 85722 0.39% 147 87007 0.39% 148 89616 0.41% 149 90173 0.41% 150 91149 0.41% 151 18443878 83.49% 22090163 reads passed initial QC criterion=sequence-density sequence-density=0.50 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=25 prefix-density=0.51 prefix-fanout=2.1 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.02 sequence-density-rank=30 fanout-score=162.23 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=13.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=23 prefix-density=0.69 prefix-fanout=2.0 sequence=AACCGCACCCCGGCACAGGCCAACATGGTTGCACCATTCAACGGCCTCAAGTCTACCTCAGCTTTCCCGGTCACCAGAAAGGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=10.12 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=1.0 sequence=GCTACACAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA SRR13695413 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:15:37 Started mapping on | Feb 12 01:15:37 Finished on | Feb 12 01:17:46 Mapping speed, Million of reads per hour | 616.47 Number of input reads | 22090163 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 21011420 Uniquely mapped reads % | 95.12% Average mapped length | 292.01 Number of splices: Total | 20370424 Number of splices: Annotated (sjdb) | 19958086 Number of splices: GT/AG | 19939462 Number of splices: GC/AG | 347889 Number of splices: AT/AC | 12479 Number of splices: Non-canonical | 70594 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 495911 % of reads mapped to multiple loci | 2.24% Number of reads mapped to too many loci | 44541 % of reads mapped to too many loci | 0.20% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.35% % of reads unmapped: other | 0.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 583081 583081 583081 N_multimapping 495911 495911 495911 N_noFeature 615332 20483561 914756 N_ambiguous 372968 2221 142940 UnstrandedReadsAssigned:20023120 PositiveStrandReadsAssigned:525638 NegativeStrandReadsAssigned:19953724 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695413 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695413-trimmed-pair1.fastq SRR13695413-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,090,163 reads, 19,978,318 reads pseudoaligned [quant] estimated average fragment length: 228.723 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,069 rounds 52401 SRR13695413.ke.tsv 34699 SRR13695413.se.tsv 87100 total ==> SRR13695413.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1790.28 521 12.7364 Potri.005G024800.1.v4.1 1035 807.277 399 21.6311 Potri.004G059700.1.v4.1 961 733.324 0 0 Potri.007G009000.2.v4.1 1416 1188.28 0 0 Potri.003G141000.2.v4.1 2943 2715.28 1158.09 18.6662 Potri.016G087400.1.v4.1 270 92.7771 1248 588.711 Potri.015G069301.1.v4.1 564 342.622 0 0 Potri.010G195200.1.v4.1 1773 1545.28 174 4.92801 Potri.012G127500.1.v4.1 977 749.297 34 1.98588 ==> SRR13695413.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 221 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 444 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2 SRR13695413 completed mapping pipeline successfully