Starting /dee2/code/volunteer_pipeline.sh SRR13695414 current disk space = 3051179864064 free memory = 1447455520 SRR13695414 SRAfilesize a52bd36249b90483853029cbed429984 SRR13695414.sra SRR13695414.sra file validated SRR13695414 is paired end SRR13695414 is conventional basespace SRR13695414 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695414_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.559 37.0 37.0 37.0 37.0 37.0 2 36.3775 37.0 37.0 37.0 37.0 37.0 3 36.5215 37.0 37.0 37.0 37.0 37.0 4 36.567 37.0 37.0 37.0 37.0 37.0 5 36.5755 37.0 37.0 37.0 37.0 37.0 6 36.562 37.0 37.0 37.0 37.0 37.0 7 36.4175 37.0 37.0 37.0 37.0 37.0 8 36.5375 37.0 37.0 37.0 37.0 37.0 9 36.578 37.0 37.0 37.0 37.0 37.0 10-14 36.5567 37.0 37.0 37.0 37.0 37.0 15-19 36.492599999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.546499999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.4441 37.0 37.0 37.0 37.0 37.0 30-34 36.3974 37.0 37.0 37.0 37.0 37.0 35-39 36.435700000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.40410000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.4071 37.0 37.0 37.0 37.0 37.0 50-54 36.3738 37.0 37.0 37.0 37.0 37.0 55-59 36.3798 37.0 37.0 37.0 37.0 37.0 60-64 36.370000000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.3228 37.0 37.0 37.0 37.0 37.0 70-74 36.309900000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.312599999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.269099999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.2577 37.0 37.0 37.0 37.0 37.0 90-94 36.142100000000006 37.0 37.0 37.0 37.0 37.0 95-99 36.119600000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.10209999999999 37.0 37.0 37.0 37.0 37.0 105-109 36.1575 37.0 37.0 37.0 37.0 37.0 110-114 36.128600000000006 37.0 37.0 37.0 37.0 37.0 115-119 36.075 37.0 37.0 37.0 37.0 37.0 120-124 35.987700000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.983000000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.99640000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.991699999999994 37.0 37.0 37.0 37.0 37.0 140-144 35.8542 37.0 37.0 37.0 37.0 37.0 145-149 35.7189 37.0 37.0 37.0 37.0 37.0 150-151 35.48425 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 1.0 25 3.0 26 5.0 27 9.0 28 12.0 29 19.0 30 28.0 31 40.0 32 53.0 33 77.0 34 114.0 35 289.0 36 2967.0 37 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.574999999999996 11.4 9.275 47.75 2 20.43630892678034 14.844533600802407 37.938816449348046 26.78034102306921 3 17.474999999999998 17.5 26.525 38.5 4 23.025000000000002 25.424999999999997 21.8 29.75 5 24.0 31.2 23.325000000000003 21.475 6 19.775000000000002 33.425 24.55 22.25 7 13.775 29.225 39.65 17.349999999999998 8 19.6 25.074999999999996 31.2 24.125 9 17.9 22.650000000000002 34.375 25.074999999999996 10-14 20.06 28.665000000000003 27.884999999999998 23.39 15-19 19.939999999999998 27.825 28.444999999999997 23.79 20-24 19.580000000000002 29.525000000000002 27.384999999999998 23.51 25-29 19.715 27.765 28.65 23.87 30-34 19.49 28.09 27.57 24.85 35-39 20.645 27.310000000000002 27.92 24.125 40-44 20.73 29.275000000000002 26.369999999999997 23.625 45-49 20.54 27.77 27.815 23.875 50-54 20.205000000000002 28.74 27.785 23.27 55-59 20.145 28.694999999999997 27.73 23.43 60-64 20.855 28.1 27.765 23.28 65-69 20.27 28.68 27.685 23.365 70-74 19.96 27.744999999999997 28.465 23.830000000000002 75-79 20.02 28.09 27.975 23.915 80-84 20.669999999999998 28.605000000000004 27.16 23.565 85-89 20.46 29.585 26.985 22.97 90-94 20.77 28.08 28.29 22.86 95-99 20.265 28.155 27.384999999999998 24.195 100-104 20.669999999999998 28.144999999999996 28.09 23.095 105-109 20.794999999999998 27.92 28.075 23.21 110-114 20.23 28.57 28.315 22.884999999999998 115-119 20.865000000000002 27.83 27.779999999999998 23.525 120-124 20.09 28.660000000000004 26.755000000000003 24.495 125-129 20.745 27.395000000000003 27.884999999999998 23.974999999999998 130-134 20.474999999999998 27.38 28.175 23.97 135-139 20.87 28.665000000000003 27.37 23.095 140-144 20.755000000000003 28.375 27.115000000000002 23.755000000000003 145-149 20.705000000000002 28.360000000000003 26.71 24.224999999999998 150-151 21.025 28.525 26.8125 23.6375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 1.0 23 1.5 24 3.0 25 4.0 26 2.5 27 4.5 28 19.0 29 22.5 30 20.5 31 22.5 32 28.5 33 40.5 34 57.0 35 71.5 36 92.0 37 108.0 38 116.5 39 152.0 40 178.5 41 197.0 42 246.0 43 255.5 44 238.5 45 249.5 46 243.0 47 247.5 48 255.5 49 217.0 50 179.0 51 150.5 52 118.0 53 103.5 54 91.5 55 73.0 56 48.5 57 34.5 58 23.0 59 18.5 60 18.5 61 11.5 62 7.0 63 6.0 64 6.0 65 2.0 66 3.5 67 4.0 68 1.0 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.3 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.67500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 66.71820641669888 43.15 2 20.873598763046 27.0 3 7.112485504445304 13.8 4 3.1696946269810593 8.200000000000001 5 1.3915732508697332 4.5 6 0.30923850019327404 1.2 7 0.27058368766911484 1.225 8 0.07730962504831851 0.4 9 0.038654812524159254 0.22499999999999998 >10 0.038654812524159254 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTAAATTGCTTCGTATATATTAATAGCTAACTTAGCACATATTTCTTCA 12 0.3 No Hit CAGAGCTCAAAACTGCCTTTGACAAAATCATAATAACCACCCTTCAGTCC 9 0.22499999999999998 No Hit CAAGCCATGCACCACCTCCAGGGCTAACTGATTGCTCAATTATAGCAACA 8 0.2 No Hit GGCTGGCTTGTACCTTCCTGGTTCTTCCACTGGCAGACCAAGAGCTCGAC 8 0.2 No Hit CCACTGGAGAACTTTATTTAGTATTCTCACATAAATAGTTCTGATCATTT 7 0.17500000000000002 No Hit GATGAGGAGCTCCCTTGAGGACATTGTTGTGGATATCAGCTTTGCCTTTC 7 0.17500000000000002 No Hit GCAGGTATGGCAACCTTTGTTGTGTTTGTTACTGACCTGAGAAAAGCCAT 7 0.17500000000000002 No Hit ATGCAGACAATGCGAAAGAAACCCATACAAACATTTTATATATCTATTGA 7 0.17500000000000002 No Hit CTTCACAATATTAGGGCAGGCATCAGTAATACTGTCGACGTGAAATTTGA 7 0.17500000000000002 No Hit CCTAGCTGACTTCTTTGGCAGCTGCGATAACAGCCTCTGTAGTAATACCG 7 0.17500000000000002 No Hit CTCTTCAACGCTGGATGTAGCCCACGAGGACAATGTCATGATCGTCCCAG 7 0.17500000000000002 No Hit GGAGAATTGGAGGGAATGGAGGTGTAGCTATTCCCATATCAGTATCTTGA 6 0.15 No Hit AACCAGTTTAAGAATGCGCTCTCGAGCAAAATATCTAGCTATCAGAAAAG 6 0.15 No Hit CGTTGCCGGCGTGCTGCTGTTGATGAAGATTGAAGAGGAAGCCCAAAGCC 6 0.15 No Hit CACCCTTCGGCTTGGAGGCGATAAAACTGATGCACTGCACTTGACGAGTG 6 0.15 No Hit TCAGTCTTCAGTGATTGTTGATGCTTGGTAAGCCTCCGGCGGATAGCTCT 6 0.15 No Hit CTATTTTCATGCATCAACTATCTCTCCAGCCTCAGCAGCAGCAAGCACAA 6 0.15 No Hit CTCAGCCACACCCCTCAATACGTGCAAAACCCCAATTTGCATAGACCGCT 6 0.15 No Hit CTCCACACCCTTCAGCTTCAACACCAGAGATCCTCAAGAGCGCATCAATA 6 0.15 No Hit CCATCTACATTCCCAGTAGTAATCTCATAAATTATTGGGCAGCCTTCGTA 5 0.125 No Hit GACCAGTGTGGTAGGCGAGATACCCATTTAGGTTTGCCTTGTGATGCAAC 5 0.125 No Hit CGTGAAATCCTTTGATTGAAGAAATTCTATGTGCTGAGGAGGAGCTTTGT 5 0.125 No Hit CTCCGGTTCCATTCCCCGCAGTAAAATCCCCTCCTTGACACATGAATCCA 5 0.125 No Hit TGGAGGAAAAATATGCGAGGGTTCCAGCTCCTGCTTCCAATCAATCACCA 5 0.125 No Hit CCCATCAAAAACACACCCAACAAAATGTCCACCACTAGCAAGCAACACAA 5 0.125 No Hit CTGCAGGATAGAATGGCATAGGAAAACTGGAAGGGCAAAAGGCAGGTGGG 5 0.125 No Hit CCAGCCTTTTCAAGATTGCACGGTTAGCTAGCCTCCTTGCACGTTCCATG 5 0.125 No Hit CTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCT 5 0.125 No Hit AGCTGCTAACATTGCTGATACATATTTGCCTAGATTGACAAGGTGATTTA 5 0.125 No Hit CACCACAGCACCACTCCTCAAGCTTTTGGGTCTTAAGCCCTGGCGAGATG 5 0.125 No Hit TGCAGGCAAGAGACAGCACCCTGAGTTGGAGGCTTGAGCACGAGGGAGTT 5 0.125 No Hit CCCAAACGGACGGACGGAAAGCATATAACACTATGAGCAAAAACCATTTG 5 0.125 No Hit GGATCGCTCTCATTTACAAGTGCAAGGATCGCAGGTACAGTTGGCTCCAC 5 0.125 No Hit CTCCCTTCAATCCTTGAGAAAGGGTTCGGTCACCAGGGCCATGAGTGTTA 5 0.125 No Hit CGACTCACAATTTACAAATTCTTTTTTGCTCTTTGAATTTCCTATCCTAA 5 0.125 No Hit TGTGATCCCATGCCCCAGTCACAAAAAAACTTGTCAAGCATGCATTTCCA 5 0.125 No Hit GGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGT 5 0.125 No Hit ATTGGGTTCTGATCAGTCAAAAGATGAATGATCTCCATGGCATGTTTGAC 5 0.125 No Hit GCAACTATGAAGAAATTTTCTGAAGAAAAACCTTGTGTGTGTAGCACGTG 5 0.125 No Hit GTGCCATTGTTGATTAATGATTGTTTGCGGAGAATATACGAGACAAAGTC 5 0.125 No Hit GTGCTGGCTTTGGACTTGCATCTCACAGGCTAGCTTTCAATACAACATAT 5 0.125 No Hit CGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCA 5 0.125 No Hit AGGGTCCTCAGCAAGCCCGAGTGGGTCAAACCCATTATCACCTGGAAGGC 5 0.125 No Hit CCTGATTCTGCCAAAATCTCTTCTCTTAGAGTTCAAAACACTCCGGGAAT 5 0.125 No Hit GCTTCAACTTGAAATTCTCATCAGCAAAGTTCTCCCCATAAATTGATTCC 5 0.125 No Hit CCAGCTGCTTTCCAGCAAAGATCAACCTTTGTTGGTCTGGGGGGATACCT 5 0.125 No Hit GTCCTAGCAGCCGATGCAGCTCTTGCTGTTGCGTACTCCCCTTCAGGATG 5 0.125 No Hit GGGCGGTGGAGCGGCACCGGACAATGGAGGCAGAAGGCAAGCGGAGGGTC 5 0.125 No Hit CGACATCGAGAACCTTACCGAGCTTAGCCTCGTTTTCCTCCACCGCTGCA 5 0.125 No Hit CCTCATCTTGTATCTGTAGCCCTTGGTAACCCCAGTGATCAAGTTCTCAA 5 0.125 No Hit CTTGGATTTGAATTTGAATTGCTCATCGATTAATTAACCGGTGAGTTCTG 5 0.125 No Hit CGTTGCCACGGGCTTACTCGCCACCGGTTTGGCCGCTACCGGTTTGGCCG 5 0.125 No Hit TGGCTGTAGACTGAATGTTCCATCTAGGGCATTTGGTTGCCCCAAAGCCT 5 0.125 No Hit CTATAGCACGCAGACCTGCCCGGGCATAACTGTCTGATGAAGGAACCCAA 5 0.125 No Hit ACCTACCCTACACTTTCTGTGGTGTTCAATACTGGAAGGAAAAATAATAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.037500000000000006 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.1125 0.0 0.0 0.0 0.0 88-89 0.1625 0.0 0.0 0.0 0.0 90-91 0.21250000000000002 0.0 0.0 0.0 0.0 92-93 0.2875 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.55 0.0 0.0 0.0 0.0 98-99 0.725 0.0 0.0 0.0 0.0 100-101 0.9125 0.0 0.0 0.0 0.0 102-103 1.125 0.0 0.0 0.0 0.0 104-105 1.2374999999999998 0.0 0.0 0.0 0.0 106-107 1.5375 0.0 0.0 0.0 0.0 108-109 1.75 0.0 0.0 0.0 0.0 110-111 1.9375 0.0 0.0 0.0 0.0 112-113 2.075 0.0 0.0 0.0 0.0 114-115 2.25 0.0 0.0 0.0 0.0 116-117 2.6 0.0 0.0 0.0 0.0 118-119 2.9124999999999996 0.0 0.0 0.0 0.0 120-121 3.075 0.0 0.0 0.0 0.0 122-123 3.4125 0.0 0.0 0.0 0.0 124-125 3.6375 0.0 0.0 0.0 0.0 126-127 3.8875 0.0 0.0 0.0 0.0 128-129 4.5375 0.0 0.0 0.0 0.0 130-131 4.9125 0.0 0.0 0.0 0.0 132-133 5.4125 0.0 0.0 0.0 0.0 134-135 5.887499999999999 0.0 0.0 0.0 0.0 136-137 6.4 0.0 0.0 0.0 0.0 138-139 6.925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATACG 10 0.006830828 145.0 4 ATACGAA 10 0.006830828 145.0 6 CGAATGC 10 0.006830828 145.0 9 TACGAAT 10 0.006830828 145.0 7 AATACGA 10 0.006830828 145.0 5 ACGAATG 10 0.006830828 145.0 8 >>END_MODULE SRR13695414 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695414_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.16825 37.0 37.0 37.0 37.0 37.0 2 36.0485 37.0 37.0 37.0 37.0 37.0 3 36.0445 37.0 37.0 37.0 37.0 37.0 4 36.0945 37.0 37.0 37.0 37.0 37.0 5 36.19 37.0 37.0 37.0 37.0 37.0 6 36.1595 37.0 37.0 37.0 37.0 37.0 7 36.103 37.0 37.0 37.0 37.0 37.0 8 36.1405 37.0 37.0 37.0 37.0 37.0 9 36.0825 37.0 37.0 37.0 37.0 37.0 10-14 36.2168 37.0 37.0 37.0 37.0 37.0 15-19 36.17380000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.09605 37.0 37.0 37.0 37.0 37.0 25-29 36.032050000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.05605 37.0 37.0 37.0 37.0 37.0 35-39 36.00725 37.0 37.0 37.0 37.0 37.0 40-44 35.98075 37.0 37.0 37.0 37.0 37.0 45-49 35.97905 37.0 37.0 37.0 37.0 37.0 50-54 35.91095 37.0 37.0 37.0 37.0 37.0 55-59 35.931050000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.87415 37.0 37.0 37.0 37.0 37.0 65-69 35.79705 37.0 37.0 37.0 37.0 37.0 70-74 35.76695 37.0 37.0 37.0 37.0 37.0 75-79 35.80425 37.0 37.0 37.0 37.0 37.0 80-84 35.77625 37.0 37.0 37.0 37.0 37.0 85-89 35.70555 37.0 37.0 37.0 37.0 37.0 90-94 35.59175 37.0 37.0 37.0 37.0 37.0 95-99 35.58835 37.0 37.0 37.0 37.0 37.0 100-104 35.65875 37.0 37.0 37.0 37.0 37.0 105-109 35.64565 37.0 37.0 37.0 37.0 37.0 110-114 35.53135 37.0 37.0 37.0 37.0 37.0 115-119 35.51735 37.0 37.0 37.0 37.0 37.0 120-124 35.41895 37.0 37.0 37.0 34.6 37.0 125-129 35.490050000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.34045 37.0 37.0 37.0 32.2 37.0 135-139 35.36845 37.0 37.0 37.0 34.6 37.0 140-144 35.177049999999994 37.0 37.0 37.0 29.8 37.0 145-149 35.114349999999995 37.0 37.0 37.0 27.4 37.0 150-151 34.744 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 2.0 14 0.0 15 1.0 16 3.0 17 3.0 18 2.0 19 0.0 20 3.0 21 2.0 22 0.0 23 4.0 24 3.0 25 3.0 26 14.0 27 11.0 28 11.0 29 28.0 30 31.0 31 52.0 32 81.0 33 126.0 34 237.0 35 661.0 36 2545.0 37 174.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.004257450538443 20.535937891309793 15.702479338842975 32.75732531930879 2 26.0 25.324999999999996 32.65 16.025 3 21.099999999999998 28.125 30.275000000000002 20.5 4 22.975 32.175 25.75 19.1 5 23.525 37.4 23.275000000000002 15.8 6 20.1 39.025 22.625 18.25 7 18.725 21.075 40.725 19.475 8 20.349999999999998 23.974999999999998 32.7 22.975 9 21.9 22.925 32.15 23.025000000000002 10-14 22.425 29.385 26.669999999999998 21.52 15-19 22.400000000000002 28.055000000000003 28.275 21.27 20-24 22.247786725353873 28.755064272495375 28.50497674185965 20.492172260291103 25-29 22.44683512634476 27.535651738804102 28.651488616462345 21.36602451838879 30-34 23.130034522439587 28.47851103217091 27.447841096712867 20.94361334867664 35-39 23.09808432951533 28.254889211223926 28.14485069774421 20.50217576151653 40-44 23.11771474310871 28.59072489869428 27.945369953474408 20.3461904047226 45-49 23.11771474310871 27.630196608134472 27.960378208014408 21.29171044074241 50-54 22.86300205071775 28.139848947131497 27.90976841894663 21.087380583204123 55-59 22.216662496872654 28.186139604703524 28.576432324243186 21.020765574180636 60-64 22.57790226579303 26.789376281698594 28.439953983894362 22.192767468614015 65-69 23.35350302545382 27.19407911186678 28.199229884482673 21.25318797819673 70-74 23.582687015261445 27.875906930197647 27.710783087315487 20.830622967225416 75-79 22.697022767075307 28.30122591943958 27.46559919939955 21.536152114085564 80-84 24.431107776944234 26.866716679169794 28.192048012003003 20.510127531882972 85-89 23.632724543407555 27.905929447085313 27.875906930197647 20.58543907930948 90-94 23.695663048371767 28.077634935721075 27.94257415837127 20.284127857535893 95-99 23.38052123455555 27.467360312140464 28.127657445850634 21.024461007453354 100-104 23.682762071553665 28.72654490868151 27.340505379034276 20.250187640730548 105-109 23.492619464598448 27.36552414310733 28.506379784838632 20.63547660745559 110-114 23.64827689691392 28.429950482668936 27.52463362176762 20.397138998649528 115-119 23.987990993244935 28.636477358018514 27.175381536152116 20.20015011258444 120-124 24.033024768576432 27.660745559169374 27.610708031023268 20.695521641230926 125-129 24.470906088957822 27.21769149947466 27.82808825736729 20.48331415420023 130-134 25.133850387790847 27.74080560420315 27.540655491618715 19.584688516387292 135-139 24.96372279209407 27.350512884663498 27.975981986489867 19.709782336752564 140-144 25.93407692692442 27.294553093582753 26.149152203271147 20.622217776221678 145-149 25.719289467100324 27.47560670502877 26.885163872904677 19.919939954966225 150-151 25.906930197648236 27.195396547410557 27.33299974981236 19.564673505128845 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 1.0 7 1.5 8 0.5 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 1.5 22 1.5 23 1.5 24 2.0 25 3.5 26 5.5 27 5.0 28 7.5 29 15.0 30 26.0 31 38.0 32 40.5 33 49.5 34 58.0 35 62.5 36 83.5 37 107.5 38 146.0 39 181.5 40 198.5 41 232.0 42 254.0 43 265.0 44 278.5 45 246.5 46 227.5 47 234.5 48 219.0 49 195.5 50 144.5 51 125.0 52 133.5 53 105.5 54 75.5 55 54.0 56 39.0 57 28.0 58 17.5 59 15.5 60 16.0 61 10.0 62 8.5 63 6.5 64 1.5 65 0.5 66 0.0 67 0.5 68 2.0 69 2.5 70 1.5 71 0.5 72 1.0 73 1.0 74 0.0 75 0.0 76 1.0 77 1.5 78 0.5 79 0.5 80 1.5 81 1.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.034999999999999996 25-29 0.075 30-34 0.065 35-39 0.034999999999999996 40-44 0.055 45-49 0.055 50-54 0.034999999999999996 55-59 0.075 60-64 0.034999999999999996 65-69 0.015 70-74 0.075 75-79 0.075 80-84 0.025 85-89 0.075 90-94 0.045 95-99 0.045 100-104 0.075 105-109 0.075 110-114 0.034999999999999996 115-119 0.075 120-124 0.075 125-129 0.065 130-134 0.075 135-139 0.075 140-144 0.034999999999999996 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.25 #Duplication Level Percentage of deduplicated Percentage of total 1 67.70114942528735 44.175 2 20.306513409961685 26.5 3 6.781609195402299 13.275 4 3.1800766283524906 8.3 5 1.1494252873563218 3.75 6 0.3448275862068966 1.35 7 0.42145593869731796 1.925 8 0.03831417624521073 0.2 9 0.03831417624521073 0.22499999999999998 >10 0.03831417624521073 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCTTGGACGGTACAGTGTCAAAACAGGAACCTGGGTTTTGAAATCAGTG 12 0.3 No Hit CTTTCCCAGACCTCTGTACACAATGTGAAAAGGAGGCAGTGAACGTGTCC 9 0.22499999999999998 No Hit TTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAG 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 7 0.17500000000000002 No Hit GCCTGAAGATGTTGCCAAGCGTCTTATGGACTATGGATTTCATGCACCTA 7 0.17500000000000002 No Hit GAAGAAATTCCAGATGGTGCAGTTGCTTTGCTGACGCCTGACATGCCTGA 7 0.17500000000000002 No Hit AAAGAATCCATCGTCTCTCGTGAAATGACCCGTCGTTACATGACAGACAT 7 0.17500000000000002 No Hit CTCCAAGATTGTTTAAGTGGGAGGATTTAACAGTTGATTTTAAAGATGGA 7 0.17500000000000002 No Hit CTGATAATGAAGATTTTGATGAAGAAAATAGCATTGAATATGGTGAAGCT 7 0.17500000000000002 No Hit TAGTTTTAATGATCTCTCTTATTTTCATCTAGCGAATACTGTTTTGTTGA 7 0.17500000000000002 No Hit AGGGAGCACTGCATAGCTTACAAGCTTGTAAGAGATGGCTTCCTCCTCTA 7 0.17500000000000002 No Hit GATAACTCCTCTAGTAATAAGCCTGATGTTATCTTGATTGGTACTGGTTC 7 0.17500000000000002 No Hit GAGTTTCTTAGCAACCAGTCTACAAAAGATCCACGATATGGCTCAACTAC 7 0.17500000000000002 No Hit GCCTTTCTTATCGCATGCATTCTCTTGGCTACCATTGTCTTCTCTCCCTT 7 0.17500000000000002 No Hit TACTCGTTGCTCTTTCTCTCCCTCTCAGTTACACTTTGTTGGTTTTTAGT 6 0.15 No Hit CAGAAGCGAAGCAATGGCAAGAATCAAGGTTCATGAGTTGAGACAGAAAT 6 0.15 No Hit GTCTTGTTGGTTGGTGTTGTTGGTGGGTTTGGTGCTGTTGGGTATATCTA 6 0.15 No Hit ATGGAAGTTTGACTTCGCTCCATTCATGGTTTTGATTATTGCTATTCTAA 6 0.15 No Hit AGCAAAGATAATCATCACTTCACTTTATTTCCTCTCTCTCTCAACAACCC 6 0.15 No Hit AAACTTTTAAGTAATGGCAGTCGCTTTCTACGATCTCACCTCAGCCGCTG 6 0.15 No Hit GTTGGGTCTACCGCGAGCACCACCAGTCCCCAGGGTACTATGATGGACGC 6 0.15 No Hit GATCACGGTCACGGTCACGGTCCAGTCTTCACTAATTCTCTTTCCTCACT 6 0.15 No Hit AGAGTATTGGCATGATTGATAGTGTTCCAGGAATGAAAGCACTTGACATG 6 0.15 No Hit CCGACCTGATGTCTCCACTGTCTGTGTTGGACTGGCAGCTAGTATGGGGG 5 0.125 No Hit GTAGTCTCCTATCAGCAAAAACAGAAAAAAGAAAGATGGCCTCGGCATCA 5 0.125 No Hit GGAATAGATTGTTTATTCTGTTTTATAGTTAAGCAGTCATTCTCTGGTTT 5 0.125 No Hit CGAAGCTGCTGAAAGGAATGCTAAAGAAATTAAGAAAGGTGTCAAAAAGT 5 0.125 No Hit AAGAAAGCGGCGGCTCCCGCTCCCGCTGTTGCTGCTGGTAAACCTGCTGC 5 0.125 No Hit AGTTAATATCTCCTGTATGCACAGATGCACTTAAGATTAAAAAACCATCT 5 0.125 No Hit TGTTACTCTGCTGTGGACTTTAGTCCTTCTTGGAACCCTAGCAGTCACCC 5 0.125 No Hit AGGGAAACCAGTTAGTCGGGAACCAAAATCAAGGCTATGGCATCACTAGC 5 0.125 No Hit AAGGCATCCCCCCAGACCAGCAGAGGTTGATTTTTGCTGGGAAGCAGCTT 5 0.125 No Hit CTGCATAGCTTACAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 5 0.125 No Hit TATGGAGGTGGTGAAAACGGTGATGACCATTCTAATGGATCTTCTGTCAC 5 0.125 No Hit GCCGGCCGGATCGTGATTGAGCTGTTCGCGGACACAACTCCACGAACCGC 5 0.125 No Hit GGTGTTTGAGAGAGAGTGAAGTGATTGAGAGGTTGAAGTTTTTGATTCAT 5 0.125 No Hit CGATCAAGTCGTTACAGCTTCCTTTGTGTGATAAGGAACATCGTTCTATC 5 0.125 No Hit AGGAATGAAAGCACTTGACATGAACGCGGCCGAAGATGCAATTGTTAGGC 5 0.125 No Hit CAATAGTTCGAGAGGGCTGCCGCCGACGCCGAGAAATCATGAAGACAATC 5 0.125 No Hit CTGCTCTTGAAGCACCAAGGGTTCTGAGCTTGAACAGCAGCAAACATTTC 5 0.125 No Hit AAGCTGTTTCTTGCGTGCTTTAACATATTGCCAAAGTTTGGTCGTGGTCG 5 0.125 No Hit GTTTGAGTTCATTCCTGTCAACATGGCGGTAGGGGAGCACAAGAAAGAGC 5 0.125 No Hit TGATAAAGAATTGAGCACTAATACACTCCACAGTCATATAGGATCAGCTT 5 0.125 No Hit GCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCA 5 0.125 No Hit CAAAAAGCATGGGCTAAAACTCCACTATGGAAGAGAGCAGAGCTTCTTCA 5 0.125 No Hit AACTGTGATTTTGCTTCTCTCATTTCTCTTCCCCTAATCTCTCTCTCTCT 5 0.125 No Hit TTCTACTTTCATCCAGGGAACTTGCTGTTTTGTGAGCATTTCTTATTTGA 5 0.125 No Hit CAGAGATGTCAGATGTGTTTGCGGACTGATTCTCTACACAAAGTGATGGA 5 0.125 No Hit GGGCAAATGAAACGATTGCTGGGAAGGAAGCTAGACCTGCCAACAGCTGG 5 0.125 No Hit CATTAATGGAGTGTTTTGCTCTGAGTTATGAACGCCGAGTTCCAATTCTT 5 0.125 No Hit CCATCACTTGCAGATGCTTGGCTGTGTTTGGGAAACTGCATCTGGAAGAA 5 0.125 No Hit GTTTAAGTGGGAGGATTTAACAGTTGATTTTAAAGATGGAGATAACCAAT 5 0.125 No Hit TTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.037500000000000006 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.1125 0.0 0.0 0.0 0.0 88-89 0.1625 0.0 0.0 0.0 0.0 90-91 0.21250000000000002 0.0 0.0 0.0 0.0 92-93 0.2875 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.55 0.0 0.0 0.0 0.0 98-99 0.725 0.0 0.0 0.0 0.0 100-101 0.9125 0.0 0.0 0.0 0.0 102-103 1.125 0.0 0.0 0.0 0.0 104-105 1.2374999999999998 0.0 0.0 0.0 0.0 106-107 1.5375 0.0 0.0 0.0 0.0 108-109 1.75 0.0 0.0 0.0 0.0 110-111 1.9375 0.0 0.0 0.0 0.0 112-113 2.075 0.0 0.0 0.0 0.0 114-115 2.25 0.0 0.0 0.0 0.0 116-117 2.6 0.0 0.0 0.0 0.0 118-119 2.9124999999999996 0.0 0.0 0.0 0.0 120-121 3.0625 0.0 0.0 0.0 0.0 122-123 3.3875 0.0 0.0 0.0 0.0 124-125 3.6375 0.0 0.0 0.0 0.0 126-127 3.875 0.0 0.0 0.0 0.0 128-129 4.512499999999999 0.0 0.0 0.0 0.0 130-131 4.9125 0.0 0.0 0.0 0.0 132-133 5.4125 0.0 0.0 0.0 0.0 134-135 5.887499999999999 0.0 0.0 0.0 0.0 136-137 6.4 0.0 0.0 0.0 0.0 138-139 6.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAAAG 10 0.006830828 145.0 8 CGGCAAA 10 0.006830828 145.0 1 CAAACCA 35 0.0033124194 62.14286 4 >>END_MODULE Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858782 spots for SRR13695414.sra Written 858782 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra Read 858764 spots for SRR13695414.sra Written 858764 spots for SRR13695414.sra SRR ids: ['SRR13695414.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_dx9ycb8m SRR13695414.sra spots: 17175298 blocks: [[1, 858764], [858765, 1717528], [1717529, 2576292], [2576293, 3435056], [3435057, 4293820], [4293821, 5152584], [5152585, 6011348], [6011349, 6870112], [6870113, 7728876], [7728877, 8587640], [8587641, 9446404], [9446405, 10305168], [10305169, 11163932], [11163933, 12022696], [12022697, 12881460], [12881461, 13740224], [13740225, 14598988], [14598989, 15457752], [15457753, 16316516], [16316517, 17175298]] SRR13695414 file size 5815217 SRR13695414 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695414 SRR13695414_1.fastq SRR13695414_2.fastq Input file: SRR13695414_1.fastq Paired file: SRR13695414_2.fastq trimmed: SRR13695414-trimmed-pair1.fastq, SRR13695414-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:12:48 2025 >> started Wed Feb 12 01:13:07 2025 >> done (19.243s) 17175298 read pairs processed; of these: 108 ( 0.00%) short read pairs filtered out after trimming by size control 1310 ( 0.01%) empty read pairs filtered out after trimming by size control 17173880 (99.99%) read pairs available; of these: 1776518 (10.34%) trimmed read pairs available after processing 15397362 (89.66%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 4 0.00% 20 2 0.00% 21 3 0.00% 22 3 0.00% 23 3 0.00% 24 1 0.00% 25 2 0.00% 26 0 0.00% 27 1 0.00% 28 5 0.00% 29 2 0.00% 30 5 0.00% 31 5 0.00% 32 4 0.00% 33 6 0.00% 34 3 0.00% 35 7 0.00% 36 5 0.00% 37 6 0.00% 38 9 0.00% 39 10 0.00% 40 10 0.00% 41 15 0.00% 42 24 0.00% 43 13 0.00% 44 25 0.00% 45 27 0.00% 46 27 0.00% 47 36 0.00% 48 35 0.00% 49 45 0.00% 50 66 0.00% 51 85 0.00% 52 75 0.00% 53 101 0.00% 54 95 0.00% 55 84 0.00% 56 148 0.00% 57 149 0.00% 58 167 0.00% 59 218 0.00% 60 245 0.00% 61 238 0.00% 62 351 0.00% 63 333 0.00% 64 379 0.00% 65 403 0.00% 66 467 0.00% 67 616 0.00% 68 608 0.00% 69 651 0.00% 70 874 0.01% 71 1062 0.01% 72 1165 0.01% 73 1267 0.01% 74 1356 0.01% 75 1650 0.01% 76 1621 0.01% 77 1883 0.01% 78 2146 0.01% 79 2509 0.01% 80 2725 0.02% 81 2984 0.02% 82 3508 0.02% 83 3797 0.02% 84 4275 0.02% 85 4603 0.03% 86 5094 0.03% 87 5193 0.03% 88 5869 0.03% 89 6111 0.04% 90 6945 0.04% 91 7483 0.04% 92 7950 0.05% 93 8269 0.05% 94 9025 0.05% 95 10030 0.06% 96 10390 0.06% 97 11138 0.06% 98 11558 0.07% 99 11964 0.07% 100 12857 0.07% 101 13063 0.08% 102 13877 0.08% 103 14921 0.09% 104 15672 0.09% 105 16286 0.09% 106 17192 0.10% 107 17575 0.10% 108 18568 0.11% 109 18984 0.11% 110 19178 0.11% 111 20345 0.12% 112 21240 0.12% 113 21764 0.13% 114 23075 0.13% 115 23544 0.14% 116 24223 0.14% 117 25183 0.15% 118 25840 0.15% 119 26688 0.16% 120 27679 0.16% 121 28651 0.17% 122 29524 0.17% 123 29448 0.17% 124 30544 0.18% 125 31915 0.19% 126 32785 0.19% 127 33694 0.20% 128 33657 0.20% 129 34473 0.20% 130 35853 0.21% 131 36430 0.21% 132 36177 0.21% 133 38091 0.22% 134 38109 0.22% 135 39264 0.23% 136 40296 0.23% 137 41286 0.24% 138 42052 0.24% 139 43123 0.25% 140 43684 0.25% 141 44720 0.26% 142 45143 0.26% 143 44983 0.26% 144 46551 0.27% 145 47406 0.28% 146 48057 0.28% 147 49387 0.29% 148 51065 0.30% 149 51030 0.30% 150 51096 0.30% 151 15397362 89.66% 17173880 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.25 fanout-score-rank=19 prefix-density=0.38 prefix-fanout=2.1 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=535.02 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=19.2 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.50 sequence-density-rank=1 fanout-score=2.08 fanout-score-rank=32 prefix-density=0.50 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=123.73 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=7.4 sequence=ACAACTTCAAGGGCAGTAGTCTTAAACCATACTCTAAAATCTTCTTATAATTCCAGTTGTAATATTCTGCTAGCATATAATGGCTTCTTCAATGAGCTTGAAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCACCACTAGCAGAAGCTGCCATCTCATGTGGCCAGGTGTCAAGCAGCTTGGCACAATGTATAACCTACCTCCAGAAGGGTGGGGCTGTGCCTGCAGCTTGCTGCAGTGGGTTGAAAGGACT SRR13695414 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:13:46 Started mapping on | Feb 12 01:13:46 Finished on | Feb 12 01:15:40 Mapping speed, Million of reads per hour | 542.33 Number of input reads | 17173880 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 16270836 Uniquely mapped reads % | 94.74% Average mapped length | 295.45 Number of splices: Total | 15894708 Number of splices: Annotated (sjdb) | 15551932 Number of splices: GT/AG | 15573980 Number of splices: GC/AG | 251393 Number of splices: AT/AC | 8467 Number of splices: Non-canonical | 60868 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.90 Insertion rate per base | 0.02% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 395092 % of reads mapped to multiple loci | 2.30% Number of reads mapped to too many loci | 24508 % of reads mapped to too many loci | 0.14% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.71% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 508142 508142 508142 N_multimapping 395092 395092 395092 N_noFeature 634733 15911323 844418 N_ambiguous 255693 1479 104782 UnstrandedReadsAssigned:15380410 PositiveStrandReadsAssigned:358034 NegativeStrandReadsAssigned:15321636 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695414 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695414-trimmed-pair1.fastq SRR13695414-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,173,880 reads, 15,242,152 reads pseudoaligned [quant] estimated average fragment length: 256.275 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,092 rounds 52401 SRR13695414.ke.tsv 34699 SRR13695414.se.tsv 87100 total ==> SRR13695414.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1762.73 422.438 14.436 Potri.005G024800.1.v4.1 1035 779.725 152 11.7428 Potri.004G059700.1.v4.1 961 705.884 4 0.341347 Potri.007G009000.2.v4.1 1416 1160.73 0 0 Potri.003G141000.2.v4.1 2943 2687.73 761.578 17.0686 Potri.016G087400.1.v4.1 270 82.9701 839 609.129 Potri.015G069301.1.v4.1 564 319.46 0 0 Potri.010G195200.1.v4.1 1773 1517.73 97 3.84988 Potri.012G127500.1.v4.1 977 721.79 157 13.1026 ==> SRR13695414.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 331 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 199 Potri.001G212900.v4.1 5 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695414 completed mapping pipeline successfully