Starting /dee2/code/volunteer_pipeline.sh SRR13695415 current disk space = 3051206762496 free memory = 1157676076 SRR13695415 SRAfilesize 09e6b504c461c821e59accc296b4bdc9 SRR13695415.sra SRR13695415.sra file validated SRR13695415 is paired end SRR13695415 is conventional basespace SRR13695415 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695415_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5715 37.0 37.0 37.0 37.0 37.0 2 36.3475 37.0 37.0 37.0 37.0 37.0 3 36.559 37.0 37.0 37.0 37.0 37.0 4 36.596 37.0 37.0 37.0 37.0 37.0 5 36.595 37.0 37.0 37.0 37.0 37.0 6 36.508 37.0 37.0 37.0 37.0 37.0 7 36.5445 37.0 37.0 37.0 37.0 37.0 8 36.5975 37.0 37.0 37.0 37.0 37.0 9 36.424 37.0 37.0 37.0 37.0 37.0 10-14 36.5458 37.0 37.0 37.0 37.0 37.0 15-19 36.5192 37.0 37.0 37.0 37.0 37.0 20-24 36.4685 37.0 37.0 37.0 37.0 37.0 25-29 36.429199999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.4459 37.0 37.0 37.0 37.0 37.0 35-39 36.409 37.0 37.0 37.0 37.0 37.0 40-44 36.415800000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.3738 37.0 37.0 37.0 37.0 37.0 50-54 36.3264 37.0 37.0 37.0 37.0 37.0 55-59 36.3224 37.0 37.0 37.0 37.0 37.0 60-64 36.321 37.0 37.0 37.0 37.0 37.0 65-69 36.2497 37.0 37.0 37.0 37.0 37.0 70-74 36.277699999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.211 37.0 37.0 37.0 37.0 37.0 80-84 36.1722 37.0 37.0 37.0 37.0 37.0 85-89 36.180099999999996 37.0 37.0 37.0 37.0 37.0 90-94 36.1406 37.0 37.0 37.0 37.0 37.0 95-99 36.0787 37.0 37.0 37.0 37.0 37.0 100-104 36.081599999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.08290000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.0223 37.0 37.0 37.0 37.0 37.0 115-119 35.9978 37.0 37.0 37.0 37.0 37.0 120-124 35.9362 37.0 37.0 37.0 37.0 37.0 125-129 35.8819 37.0 37.0 37.0 37.0 37.0 130-134 35.8538 37.0 37.0 37.0 37.0 37.0 135-139 35.7732 37.0 37.0 37.0 37.0 37.0 140-144 35.7202 37.0 37.0 37.0 37.0 37.0 145-149 35.4649 37.0 37.0 37.0 37.0 37.0 150-151 35.310500000000005 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 4.0 25 4.0 26 6.0 27 13.0 28 15.0 29 23.0 30 28.0 31 37.0 32 42.0 33 76.0 34 135.0 35 320.0 36 2956.0 37 339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.15 11.575000000000001 8.75 40.525 2 21.027568922305765 13.508771929824562 34.78696741854637 30.67669172932331 3 19.325 18.5 25.1 37.075 4 22.15 26.525 22.3 29.025000000000002 5 22.725 32.65 23.325000000000003 21.3 6 22.125 34.275 23.599999999999998 20.0 7 17.875 26.1 40.625 15.4 8 18.475 26.35 30.725 24.45 9 18.0 24.15 34.699999999999996 23.150000000000002 10-14 19.8 29.360000000000003 27.900000000000002 22.939999999999998 15-19 19.43 27.85 28.62 24.099999999999998 20-24 20.225 28.29 28.265 23.22 25-29 20.02 28.46 28.294999999999998 23.225 30-34 19.525000000000002 28.804999999999996 27.689999999999998 23.98 35-39 20.349999999999998 28.29 28.74 22.62 40-44 20.25 29.005 27.195000000000004 23.549999999999997 45-49 19.8 28.415000000000003 27.755000000000003 24.03 50-54 19.825 28.299999999999997 27.01 24.865000000000002 55-59 20.685000000000002 28.544999999999998 27.445000000000004 23.325000000000003 60-64 19.869999999999997 28.73 28.055000000000003 23.345 65-69 19.495 28.58 27.63 24.295 70-74 20.09 28.335 28.175 23.400000000000002 75-79 20.22 28.055000000000003 27.650000000000002 24.075 80-84 19.509999999999998 28.79 27.525 24.175 85-89 20.674999999999997 28.189999999999998 27.875 23.26 90-94 21.279999999999998 28.16 27.245 23.315 95-99 20.075000000000003 28.49 27.775 23.66 100-104 20.57 28.575 27.325 23.53 105-109 20.325 28.105000000000004 27.744999999999997 23.825 110-114 20.27 29.165000000000003 27.310000000000002 23.255 115-119 20.235 28.025 27.839999999999996 23.9 120-124 20.535 28.13 27.205000000000002 24.13 125-129 20.674999999999997 28.665000000000003 27.245 23.415 130-134 21.185000000000002 28.305000000000003 27.084999999999997 23.425 135-139 20.655 29.365000000000002 26.290000000000003 23.69 140-144 20.815 27.54 27.83 23.815 145-149 20.665 28.46 27.355 23.52 150-151 20.1875 28.575 27.9125 23.325000000000003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 1.0 3 1.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 4.0 25 5.5 26 3.0 27 6.0 28 12.5 29 20.0 30 20.0 31 22.5 32 31.0 33 35.0 34 43.5 35 69.5 36 92.5 37 101.0 38 116.0 39 140.0 40 182.5 41 221.5 42 258.0 43 272.0 44 275.5 45 284.5 46 263.5 47 254.0 48 235.5 49 190.0 50 161.5 51 157.0 52 128.0 53 87.0 54 78.0 55 60.5 56 34.0 57 36.5 58 34.5 59 17.0 60 12.5 61 11.0 62 6.0 63 2.5 64 2.0 65 1.5 66 2.0 67 1.5 68 2.0 69 1.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.25 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.975 #Duplication Level Percentage of deduplicated Percentage of total 1 71.81136120042872 50.24999999999999 2 19.006788138620937 26.6 3 5.966416577349054 12.525 4 1.5362629510539478 4.3 5 1.1789924973204717 4.125 6 0.35727045373347627 1.5 7 0.1429081814933905 0.7000000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGGGCGACAAGGATCCTTGATGCCAACAATAGCAAGCAAAACAAGCTCA 7 0.17500000000000002 No Hit CCTATTTCTTGTACATGTAAAATTAACTAGTCCACAGGCTCTACAGACGG 7 0.17500000000000002 No Hit GGACACATCAAGTTCTGGGACGACTTAGACACCTTTCGGCTTGGCGGCAA 7 0.17500000000000002 No Hit CCCCTTCAAGCAAAACTCAAATGCAGCCCACAAAACTGAACTCTCAGCTG 7 0.17500000000000002 No Hit CGTCAGTACTGGCTGTTTCCAAGAGAAAGGCAACTTGATCATGCTCATGC 6 0.15 No Hit CAATCAAGGCCACAATGCGAGCATAAATAACTTAAATATACCTTCACTCT 6 0.15 No Hit CCCAATCAGCGGTACTTGATGAAGCCTATTTCCTTGGCATTGCTGCGGAA 6 0.15 No Hit GTGATGACTTGAGAAATGATGCCGAGGCCATCTTTCTTTTTTCTGTTTTT 6 0.15 No Hit TTTTCTCTTTAAAAATAATATTAATAAATAATAAAGTTGAGAATCATCGT 6 0.15 No Hit GCTTCAACAGGTCTCTGTCTTCGGGGCTCAAAGCGGATGGTCATTGTGTG 6 0.15 No Hit CACCACATCACTAGGTAGCAAAAGAGACACTCCTTTTGCCTTGGCCTTCT 6 0.15 No Hit CGTCAATGTCAAGTTCCTCAAGCTCCAACCACTTAACAGTGTCATAGCCT 6 0.15 No Hit GCTCAATCTTTCCCAAGGAATTCCCTGGATATCCTTTCCCTTCCGAGCAT 6 0.15 No Hit GGCCCAGCTGCTTTCCCTATCACTGAATCATAAGTATATAAAGTTCCTAT 6 0.15 No Hit GTCCAGTTGTACAGACCTAGGATCAAGGGGGATAGTACCCACTGTTAGAA 5 0.125 No Hit AAGGAAGACTATGCCATTTTACGTGAAAATAAGATATGTTTTAAAGGTTC 5 0.125 No Hit CAGGAACAACTTAAGCACACTAATAAAGCAGAATTGCACATATTCACAGC 5 0.125 No Hit CGGGTGTTGAGTAGTCTTTAATGGCCTCTTGGAATGCTTGAAGCATTGCA 5 0.125 No Hit CGCTTAAATAATTTAGTATCGAAATTTCAAAAAGCCAAAGTCTCTCAACT 5 0.125 No Hit CGGGAAAAGAAAGTTGAGGAAATTTGTCAAGTTCGTGAGGTAGAAAGATA 5 0.125 No Hit CTCGGTGTTCTCTAGCCCGATTGAGGCGAGACGTTTTCCACAGGTAGCGT 5 0.125 No Hit CTATATCTTTCTGCTCCACCCCTAGAAGACTATATGTCTCATTCATCGGA 5 0.125 No Hit CTGTAGTCAATCTAGTCCTCTTGGAATGGCGCATACATTTGATAGCTTCC 5 0.125 No Hit TAGGATTCAGATTAACAGGAGAAGGGACAGCTGTGTGTGGTGACATCGCA 5 0.125 No Hit CTTCAAACACATGACCACGTTTCTGGTTAAGAACACTGTAGATGCCACCA 5 0.125 No Hit TCTGAAGTATGACTCTCACTCCATTTTGGATCATTCCATGATTTTTCCAC 5 0.125 No Hit CCGCGGAGAAGTGGACTGACTGCTGTCCTGTAGGCGGGGAGGTTGGACAG 5 0.125 No Hit GTCCTTGTGGGTCCAGTGGTTGTAAGGAATGGCCAACGCAAACATGAACA 5 0.125 No Hit ATTCCCACAGCTATTCTTTTGAAGGTGGTCTCTTGATCTTATATCAGCCT 5 0.125 No Hit CCTTTTGAGCCCACCAGATTTCTTTAACGCCATTGAAATGATTCAACCAA 5 0.125 No Hit GCAATGGATGAGTGACCTGGCTGAGACTTATTTGAGTTCTTCGTATGAGT 5 0.125 No Hit GCCCAAATTGTTGATTTAAATGTACCTCGTTCTCTCTTGTACTTGTCTTG 5 0.125 No Hit GTTCTTGCATTGGTTAAGAAGTACTCCTTTATTGATCCTACGCCAAGCAT 5 0.125 No Hit CGAGAATATTGCAAACCTTAACTTCATCTCCCCCTCTGTCTCATACCTCT 5 0.125 No Hit ACCAGGAGCAATGTCAGTTGTTAGAGGTCCAAGAGTATAGAAAGGAGCTT 5 0.125 No Hit TGTATGACTTGGGGAAAAAGAGGTTGCTTAGGAAGTGTGAGAATAAGCTG 5 0.125 No Hit GTTTCAGTGAGGTTCTTCTTCTCTATAGCACATCCAAGTCTAGCTCTGAG 5 0.125 No Hit CCCGGATCCAAGCAAACGGGTTGCGGGATCTTGACGCGCCATCCCAGGGT 5 0.125 No Hit GCATGGTGATATCTATATAAATAAGGAGTCCCCAATTGAAACAAGGGGGC 5 0.125 No Hit CTCCTCTCTCTCTCTCCCTCTCTATCCTTTCCCTCTCCTTCTCTTTCTCT 5 0.125 No Hit CCCTGGGCTTCCTTCCAGATGCATCTAACCCGTTCCTCAGGTTCATGCCA 5 0.125 No Hit CCTGTCTCGATGTTCCCTGCTACTCTTGTGCCGATCCCTCTCTCCATCTC 5 0.125 No Hit CCTAGAAAAGGAATTAGAATTTGTAAGTGCAGCTTCCATCATCACTCCTT 5 0.125 No Hit GAGCTGTCTGCCTGTATGGATGAGGGGGACTCTGTTTGGCTTGGCACTGC 5 0.125 No Hit CCAATCTTCTCAAACTTATGGTTTATGCTCTTCTTTTGCTTGAGCCACAC 5 0.125 No Hit GTCAACATATCCAATAATACAAAAAAAACATTAGCATTTATCAATTGAAG 5 0.125 No Hit CACTACTGTAACCCCCAGCCTTTTCAACCAAGAACCCCAATGGCGCAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0125 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.575 0.0 0.0 0.0 0.0 94-95 0.7124999999999999 0.0 0.0 0.0 0.0 96-97 0.875 0.0 0.0 0.0 0.0 98-99 0.9125000000000001 0.0 0.0 0.0 0.0 100-101 0.95 0.0 0.0 0.0 0.0 102-103 1.2000000000000002 0.0 0.0 0.0 0.0 104-105 1.4500000000000002 0.0 0.0 0.0 0.0 106-107 1.6749999999999998 0.0 0.0 0.0 0.0 108-109 1.9874999999999998 0.0 0.0 0.0 0.0 110-111 2.3625 0.0 0.0 0.0 0.0 112-113 2.7875 0.0 0.0 0.0 0.0 114-115 3.0375 0.0 0.0 0.0 0.0 116-117 3.2625 0.0 0.0 0.0 0.0 118-119 3.4625 0.0 0.0 0.0 0.0 120-121 3.775 0.0 0.0 0.0 0.0 122-123 4.3625 0.0 0.0 0.0 0.0 124-125 4.8375 0.0 0.0 0.0 0.0 126-127 5.3375 0.0 0.0 0.0 0.0 128-129 5.775 0.0 0.0 0.0 0.0 130-131 6.2875 0.0 0.0 0.0 0.0 132-133 6.574999999999999 0.0 0.0 0.0 0.0 134-135 7.075 0.0 0.0 0.0 0.0 136-137 7.5875 0.0 0.0 0.0 0.0 138-139 8.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCCAA 10 0.006830828 145.0 1 GCTAGTA 10 0.006830828 145.0 2 CCAAATG 10 0.006830828 145.0 4 GTATCCA 10 0.006830828 145.0 6 AGTATCC 10 0.006830828 145.0 5 GCCACTT 10 0.006830828 145.0 145 GGCTAGT 10 0.006830828 145.0 1 >>END_MODULE SRR13695415 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695415_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.04675 37.0 37.0 37.0 37.0 37.0 2 36.099 37.0 37.0 37.0 37.0 37.0 3 36.098 37.0 37.0 37.0 37.0 37.0 4 36.203 37.0 37.0 37.0 37.0 37.0 5 36.228 37.0 37.0 37.0 37.0 37.0 6 36.1085 37.0 37.0 37.0 37.0 37.0 7 36.25 37.0 37.0 37.0 37.0 37.0 8 36.1675 37.0 37.0 37.0 37.0 37.0 9 36.152 37.0 37.0 37.0 37.0 37.0 10-14 36.1141 37.0 37.0 37.0 37.0 37.0 15-19 36.1229 37.0 37.0 37.0 37.0 37.0 20-24 36.113099999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.005 37.0 37.0 37.0 37.0 37.0 30-34 36.01520000000001 37.0 37.0 37.0 37.0 37.0 35-39 35.9735 37.0 37.0 37.0 37.0 37.0 40-44 35.943599999999996 37.0 37.0 37.0 37.0 37.0 45-49 35.962700000000005 37.0 37.0 37.0 37.0 37.0 50-54 35.8568 37.0 37.0 37.0 37.0 37.0 55-59 35.8307 37.0 37.0 37.0 37.0 37.0 60-64 35.822500000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.7881 37.0 37.0 37.0 37.0 37.0 70-74 35.7692 37.0 37.0 37.0 37.0 37.0 75-79 35.8048 37.0 37.0 37.0 37.0 37.0 80-84 35.7996 37.0 37.0 37.0 37.0 37.0 85-89 35.67139999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.6143 37.0 37.0 37.0 37.0 37.0 95-99 35.6667 37.0 37.0 37.0 37.0 37.0 100-104 35.6366 37.0 37.0 37.0 37.0 37.0 105-109 35.5517 37.0 37.0 37.0 37.0 37.0 110-114 35.514599999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.4803 37.0 37.0 37.0 37.0 37.0 120-124 35.423700000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.5157 37.0 37.0 37.0 37.0 37.0 130-134 35.292500000000004 37.0 37.0 37.0 32.2 37.0 135-139 35.3695 37.0 37.0 37.0 34.6 37.0 140-144 35.1976 37.0 37.0 37.0 29.8 37.0 145-149 35.11919999999999 37.0 37.0 37.0 27.4 37.0 150-151 34.786500000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 4.0 15 1.0 16 3.0 17 1.0 18 1.0 19 2.0 20 1.0 21 1.0 22 2.0 23 6.0 24 4.0 25 10.0 26 14.0 27 19.0 28 19.0 29 32.0 30 31.0 31 54.0 32 79.0 33 116.0 34 222.0 35 589.0 36 2608.0 37 179.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.72465581977472 20.826032540675847 14.292866082603254 25.15644555694618 2 27.075 25.25 31.65 16.025 3 20.375 27.750000000000004 31.4 20.474999999999998 4 22.275 35.025 23.45 19.25 5 25.650000000000002 35.225 21.8 17.325 6 21.475 38.550000000000004 22.650000000000002 17.325 7 19.950000000000003 21.975 38.6 19.475 8 20.375 26.5 29.425 23.7 9 23.799999999999997 24.45 30.325000000000003 21.425 10-14 23.29 29.770000000000003 26.715 20.225 15-19 22.805 28.935 27.750000000000004 20.51 20-24 23.41 27.87 28.37 20.349999999999998 25-29 22.705000000000002 27.97 28.57 20.755000000000003 30-34 22.24 28.794999999999998 28.04 20.925 35-39 22.189999999999998 28.494999999999997 28.055000000000003 21.26 40-44 22.165000000000003 28.645 28.050000000000004 21.14 45-49 23.085 28.175 28.705000000000002 20.035 50-54 22.065 27.865000000000002 28.38 21.69 55-59 23.46 27.83 28.23 20.48 60-64 22.95 28.345 27.775 20.93 65-69 22.470000000000002 28.615000000000002 28.110000000000003 20.805 70-74 22.564999999999998 28.465 28.325 20.645 75-79 23.01 27.43 28.58 20.979999999999997 80-84 22.295 28.585 27.85 21.27 85-89 23.835 27.860000000000003 26.71 21.595 90-94 22.955000000000002 28.08 28.29 20.674999999999997 95-99 22.35 29.01 27.97 20.669999999999998 100-104 23.474999999999998 28.73 27.21 20.585 105-109 23.330000000000002 27.6 28.89 20.18 110-114 24.044999999999998 28.685 27.034999999999997 20.235 115-119 24.11 28.410000000000004 26.205000000000002 21.275 120-124 24.615000000000002 28.37 27.339999999999996 19.675 125-129 24.355 27.41 28.055000000000003 20.18 130-134 24.91 28.315 26.740000000000002 20.035 135-139 24.46 27.765 27.97 19.805 140-144 24.8 27.544999999999998 27.165 20.49 145-149 25.119999999999997 28.139999999999997 27.05 19.689999999999998 150-151 25.6 28.3125 26.387500000000003 19.7 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.0 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 3.5 19 3.0 20 0.0 21 1.5 22 3.5 23 2.5 24 2.5 25 4.5 26 9.0 27 11.5 28 9.0 29 15.5 30 26.0 31 30.5 32 31.0 33 31.5 34 50.0 35 78.5 36 103.0 37 128.0 38 125.5 39 160.0 40 217.5 41 236.0 42 254.0 43 261.0 44 274.0 45 290.5 46 272.5 47 227.0 48 196.5 49 175.0 50 164.5 51 134.5 52 98.5 53 81.5 54 67.5 55 49.5 56 29.0 57 30.0 58 26.0 59 23.5 60 20.5 61 10.0 62 6.5 63 4.0 64 2.0 65 3.0 66 1.0 67 1.5 68 1.5 69 0.5 70 1.0 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.65 #Duplication Level Percentage of deduplicated Percentage of total 1 72.92993630573248 51.525 2 17.8343949044586 25.2 3 6.015569709837226 12.75 4 1.875442321302194 5.3 5 0.8846426043878274 3.125 6 0.3184713375796179 1.35 7 0.10615711252653928 0.525 8 0.0 0.0 9 0.035385704175513094 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAGATTGTGAAAAAAGAAAGGCAGAAGCAAGTTCAGTAATGGCAGCCTC 9 0.22499999999999998 No Hit TGATGACATGGCTGCCTGCAGCTTGCGATGTGTTGCTATTGCATACAGAC 7 0.17500000000000002 No Hit GTTGGAGAAAGGTTGGGTCTACCGTGAGCACCACAGCTCACCAGGGTACT 7 0.17500000000000002 No Hit CTAAAGTTAGCATTTTTAACGTGTTTCCGGCAAGCTCTCCCTAAAGTTAG 7 0.17500000000000002 No Hit TTTCAAACCCAAAGAGGCCATTTGCTGCCATTGTGGGTGGTTCTAAGGTC 6 0.15 No Hit GTGGAAATGGAAGATCTTATGTTTTGATACTCGAAGCTGGTCCTTCAGCT 6 0.15 No Hit GATAAGATATAGACACTTGTTATAGGCTATCTTGCACTGGCAGTGTAGTC 6 0.15 No Hit GGAAAGGTGCCCTCTTTTCTCTTAGCGTTTCTTCTCCAAGCTCAAAGATT 6 0.15 No Hit AGAAATCGAAAGCTGAGGACGAGAAAGGTCGCGATCACCACCGGAGAGAC 6 0.15 No Hit TGCACTCGAACTGTTCAAAGCTCTCTAGCTAGCTAGTTTCCATATTCGGT 6 0.15 No Hit AGTGGCACTTGAGAGCGGCGCAGCGAGTTAGGGTTTTCTCCGTCGATTAA 6 0.15 No Hit GAGTGTTTGTCGCTACCACCTACATACTTGATGGGCCTTTCAGTTTCATC 6 0.15 No Hit GAAGGTTCGATTGAGTCAGCCGCAGATCACATCTTAATCCAAAGAGATCT 6 0.15 No Hit TGGTAATAAAAAAAATTTATTCTTTTTTAGTACCAAATTTGCCTTTGCTA 5 0.125 No Hit ACAGAGGTGGTGGGCAGGTCATTCCTACTGCCAGGAGGGTTATCTATGCC 5 0.125 No Hit CTTGAGGCGGAAGAGCAAAAGGTCGAGCAACTGACCGAGGACAAGTCCAA 5 0.125 No Hit GTTCGCAGGAAGAAATGCGTGGAGTCATATCCAAGGCATACTTCTTCCAG 5 0.125 No Hit TTCTAAGGCAGAATTGAAGGAGAAGCTGGCAAGTTTGTATGAGGTGAAGG 5 0.125 No Hit GTACGGTTACTACCCTGCTTGCTATCCTTCACAACCTCCCGTACCATCTC 5 0.125 No Hit GTGAGGGCCAATGGTCATGGGAATAAGCGTGGGTGGAGTTCATGGTTTGA 5 0.125 No Hit TCCCTTTAACTGCAAAGCGCATGACAGGAATTGTTTCGCGAGGAGGATCA 5 0.125 No Hit AGGCTATCTTGCACTGGCAGTGTAGTCTCCTATCAGCAAAAACAGAAAAA 5 0.125 No Hit TGTGAAGAGAGTCCAATTGGCTGACAAGTACCTGAGCGAGGCTTCTCTTG 5 0.125 No Hit CCCCCAACCACGTATGCAATATGATGACAAGAGTCGGGTGGAAAAGGCAA 5 0.125 No Hit TGAATGGAATGCAAGAACACAAGTGACAATGTGGTATGATACGACAAAAA 5 0.125 No Hit AAGAGTTTCTTATTGCAAACAAAGTCAGAAACTCAAGATGTCAAGGAACT 5 0.125 No Hit GAGGAAGCAAAGAAAATGAGCATAGTAGCGAAAGAGACAATCGAAGTGAT 5 0.125 No Hit CGTCTATGTGGTTTCATGCAAGGCCTTATTGGGCAATATGCAGTACCTAT 5 0.125 No Hit CAATGGGGAGTGGAAGATCTGCTGATTGCTGTAATGAGTTTCTAAAAGGA 5 0.125 No Hit ATTCTGGGCAAGTTACAGTGGATGCAGATGATGATATTGGATCTGTGCTA 5 0.125 No Hit CGGAAAAAGAGGTTGGGAAGGTGAGCATGGAGTGGTTGGCTGGGGAGAAA 5 0.125 No Hit TCCTAAAGAAGTTAAAACAACTTAACAAGTTTTCTGAGGAAAATGTCTCT 5 0.125 No Hit TTTTTTTTTTCTTGATATTATTAATTACGTCAAGACTTTTTTCAGTAAGA 5 0.125 No Hit GTTAAAGAGAAGGGTGTCTTCACAAATGTTATTTCACCATCTTCCAAAGC 5 0.125 No Hit TGATGAACAAAGAAGGCGAACTTGGACTACTGCCAAACCATTTCTTAATG 5 0.125 No Hit GCCTGTACTTGCAGAAATGAAATGATTCTTGAATGTCACCCACATAAATC 5 0.125 No Hit GTTCTCAGGGTGTTTACTATTATTTCTATCAAATATTCAGGGATAGAGCT 5 0.125 No Hit CTATGTGAAGCTGTGGCAACATGGGAACAGGTGCGGAATAGTACTACAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0125 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.425 0.0 0.0 0.0 0.0 92-93 0.575 0.0 0.0 0.0 0.0 94-95 0.7124999999999999 0.0 0.0 0.0 0.0 96-97 0.875 0.0 0.0 0.0 0.0 98-99 0.9125000000000001 0.0 0.0 0.0 0.0 100-101 0.95 0.0 0.0 0.0 0.0 102-103 1.2000000000000002 0.0 0.0 0.0 0.0 104-105 1.4249999999999998 0.0 0.0 0.0 0.0 106-107 1.65 0.0 0.0 0.0 0.0 108-109 1.9625 0.0 0.0 0.0 0.0 110-111 2.3625 0.0 0.0 0.0 0.0 112-113 2.8375 0.0 0.0 0.0 0.0 114-115 3.0875 0.0 0.0 0.0 0.0 116-117 3.325 0.0 0.0 0.0 0.0 118-119 3.5625 0.0 0.0 0.0 0.0 120-121 3.875 0.0 0.0 0.0 0.0 122-123 4.475 0.0 0.0 0.0 0.0 124-125 4.9625 0.0 0.0 0.0 0.0 126-127 5.449999999999999 0.0 0.0 0.0 0.0 128-129 5.9 0.0 0.0 0.0 0.0 130-131 6.4125 0.0 0.0 0.0 0.0 132-133 6.699999999999999 0.0 0.0 0.0 0.0 134-135 7.2 0.0 0.0 0.0 0.0 136-137 7.6875 0.0 0.0 0.0 0.0 138-139 8.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGGGA 10 0.006830828 145.0 7 GATCTTC 10 0.006830828 145.0 1 CAAAGTG 10 0.006830828 145.0 145 TTCTAGG 10 0.006830828 145.0 5 TTTATGT 10 0.006830828 145.0 3 CTTCTAG 10 0.006830828 145.0 4 TTATGTC 10 0.006830828 145.0 4 AGGGAAG 30 0.0017973486 72.5 9 >>END_MODULE Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra Read 750595 spots for SRR13695415.sra Written 750595 spots for SRR13695415.sra Read 750576 spots for SRR13695415.sra Written 750576 spots for SRR13695415.sra SRR ids: ['SRR13695415.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_o84d5b9x SRR13695415.sra spots: 15011539 blocks: [[1, 750576], [750577, 1501152], [1501153, 2251728], [2251729, 3002304], [3002305, 3752880], [3752881, 4503456], [4503457, 5254032], [5254033, 6004608], [6004609, 6755184], [6755185, 7505760], [7505761, 8256336], [8256337, 9006912], [9006913, 9757488], [9757489, 10508064], [10508065, 11258640], [11258641, 12009216], [12009217, 12759792], [12759793, 13510368], [13510369, 14260944], [14260945, 15011539]] SRR13695415 file size 5079877 SRR13695415 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695415 SRR13695415_1.fastq SRR13695415_2.fastq Input file: SRR13695415_1.fastq Paired file: SRR13695415_2.fastq trimmed: SRR13695415-trimmed-pair1.fastq, SRR13695415-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:16:03 2025 >> started Wed Feb 12 01:16:21 2025 >> done (18.643s) 15011539 read pairs processed; of these: 121 ( 0.00%) short read pairs filtered out after trimming by size control 1962 ( 0.01%) empty read pairs filtered out after trimming by size control 15009456 (99.99%) read pairs available; of these: 1642456 (10.94%) trimmed read pairs available after processing 13367000 (89.06%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 2 0.00% 20 0 0.00% 21 0 0.00% 22 3 0.00% 23 0 0.00% 24 1 0.00% 25 2 0.00% 26 1 0.00% 27 4 0.00% 28 3 0.00% 29 2 0.00% 30 5 0.00% 31 1 0.00% 32 6 0.00% 33 5 0.00% 34 1 0.00% 35 7 0.00% 36 6 0.00% 37 12 0.00% 38 5 0.00% 39 8 0.00% 40 5 0.00% 41 18 0.00% 42 11 0.00% 43 18 0.00% 44 9 0.00% 45 21 0.00% 46 19 0.00% 47 20 0.00% 48 40 0.00% 49 45 0.00% 50 49 0.00% 51 67 0.00% 52 94 0.00% 53 70 0.00% 54 107 0.00% 55 94 0.00% 56 96 0.00% 57 108 0.00% 58 124 0.00% 59 166 0.00% 60 205 0.00% 61 231 0.00% 62 265 0.00% 63 338 0.00% 64 401 0.00% 65 398 0.00% 66 419 0.00% 67 499 0.00% 68 569 0.00% 69 682 0.00% 70 866 0.01% 71 928 0.01% 72 1045 0.01% 73 1205 0.01% 74 1433 0.01% 75 1619 0.01% 76 1770 0.01% 77 1812 0.01% 78 2190 0.01% 79 2449 0.02% 80 2698 0.02% 81 2932 0.02% 82 3541 0.02% 83 3768 0.03% 84 4199 0.03% 85 4811 0.03% 86 5162 0.03% 87 5460 0.04% 88 5614 0.04% 89 6047 0.04% 90 6348 0.04% 91 7080 0.05% 92 7489 0.05% 93 8358 0.06% 94 8948 0.06% 95 9732 0.06% 96 10365 0.07% 97 10756 0.07% 98 10868 0.07% 99 11719 0.08% 100 12197 0.08% 101 12695 0.08% 102 13416 0.09% 103 14007 0.09% 104 15072 0.10% 105 15547 0.10% 106 16540 0.11% 107 16817 0.11% 108 17392 0.12% 109 18111 0.12% 110 18171 0.12% 111 19047 0.13% 112 19904 0.13% 113 20430 0.14% 114 21700 0.14% 115 22414 0.15% 116 22796 0.15% 117 23589 0.16% 118 24283 0.16% 119 24643 0.16% 120 25523 0.17% 121 26596 0.18% 122 26849 0.18% 123 27924 0.19% 124 28942 0.19% 125 29327 0.20% 126 30411 0.20% 127 31027 0.21% 128 31448 0.21% 129 31718 0.21% 130 32789 0.22% 131 32838 0.22% 132 33823 0.23% 133 34358 0.23% 134 35388 0.24% 135 35917 0.24% 136 37304 0.25% 137 37612 0.25% 138 37723 0.25% 139 39741 0.26% 140 39496 0.26% 141 40227 0.27% 142 40580 0.27% 143 41156 0.27% 144 42201 0.28% 145 42582 0.28% 146 43552 0.29% 147 44419 0.30% 148 44786 0.30% 149 45236 0.30% 150 45712 0.30% 151 13367000 89.06% 15009456 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.60 fanout-score-rank=31 prefix-density=0.29 prefix-fanout=2.3 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCACCAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=482.83 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=18.9 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=sequence-density sequence-density=0.62 sequence-density-rank=1 fanout-score=2.26 fanout-score-rank=26 prefix-density=0.64 prefix-fanout=2.2 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.08 sequence-density-rank=24 fanout-score=59.97 fanout-score-rank=1 prefix-density=0.49 prefix-fanout=10.3 sequence=AGAAAAGAAAACAGATTATCAAGCTTACTAGAATTATGGAAGGAATGAGTGTGGAGAACATGCACAAGATAGTGGTGGCAGTGGATGAGAGTGAGGAGAGCATGCATGCTCTTTCATGGTGTCTCAGCAACCTTATTTCTCA SRR13695415 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:17:05 Started mapping on | Feb 12 01:17:06 Finished on | Feb 12 01:18:57 Mapping speed, Million of reads per hour | 486.79 Number of input reads | 15009456 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 13943875 Uniquely mapped reads % | 92.90% Average mapped length | 295.03 Number of splices: Total | 13482301 Number of splices: Annotated (sjdb) | 13171656 Number of splices: GT/AG | 13207826 Number of splices: GC/AG | 214014 Number of splices: AT/AC | 8247 Number of splices: Non-canonical | 52214 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 338896 % of reads mapped to multiple loci | 2.26% Number of reads mapped to too many loci | 94971 % of reads mapped to too many loci | 0.63% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.03% % of reads unmapped: other | 0.17% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 726857 726857 726857 N_multimapping 338896 338896 338896 N_noFeature 644559 13586878 880973 N_ambiguous 203648 1598 81894 UnstrandedReadsAssigned:13095668 PositiveStrandReadsAssigned:355399 NegativeStrandReadsAssigned:12981008 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695415 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695415-trimmed-pair1.fastq SRR13695415-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,009,456 reads, 13,031,479 reads pseudoaligned [quant] estimated average fragment length: 257.492 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,137 rounds 52401 SRR13695415.ke.tsv 34699 SRR13695415.se.tsv 87100 total ==> SRR13695415.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1761.51 435 18.0483 Potri.005G024800.1.v4.1 1035 778.508 236 22.1555 Potri.004G059700.1.v4.1 961 704.614 3 0.311173 Potri.007G009000.2.v4.1 1416 1159.51 0 0 Potri.003G141000.2.v4.1 2943 2686.51 954 25.9533 Potri.016G087400.1.v4.1 270 84.8774 452 389.205 Potri.015G069301.1.v4.1 564 320.117 0 0 Potri.010G195200.1.v4.1 1773 1516.51 121 5.83139 Potri.012G127500.1.v4.1 977 720.553 120 12.1716 ==> SRR13695415.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 112 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 174 Potri.001G212900.v4.1 9 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 18 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 10 SRR13695415 completed mapping pipeline successfully