Starting /dee2/code/volunteer_pipeline.sh SRR13695416 current disk space = 3050882551808 free memory = 1461917412 SRR13695416 SRAfilesize b7578921c74b9e796207d91bf7a93d9f SRR13695416.sra SRR13695416.sra file validated SRR13695416 is paired end SRR13695416 is conventional basespace SRR13695416 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695416_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.601 37.0 37.0 37.0 37.0 37.0 2 36.453 37.0 37.0 37.0 37.0 37.0 3 36.478 37.0 37.0 37.0 37.0 37.0 4 36.6225 37.0 37.0 37.0 37.0 37.0 5 36.536 37.0 37.0 37.0 37.0 37.0 6 36.549 37.0 37.0 37.0 37.0 37.0 7 36.5165 37.0 37.0 37.0 37.0 37.0 8 36.521 37.0 37.0 37.0 37.0 37.0 9 36.4805 37.0 37.0 37.0 37.0 37.0 10-14 36.5487 37.0 37.0 37.0 37.0 37.0 15-19 36.4916 37.0 37.0 37.0 37.0 37.0 20-24 36.5205 37.0 37.0 37.0 37.0 37.0 25-29 36.4967 37.0 37.0 37.0 37.0 37.0 30-34 36.3972 37.0 37.0 37.0 37.0 37.0 35-39 36.4197 37.0 37.0 37.0 37.0 37.0 40-44 36.386799999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.4106 37.0 37.0 37.0 37.0 37.0 50-54 36.363099999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3018 37.0 37.0 37.0 37.0 37.0 60-64 36.3291 37.0 37.0 37.0 37.0 37.0 65-69 36.2663 37.0 37.0 37.0 37.0 37.0 70-74 36.286500000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.2581 37.0 37.0 37.0 37.0 37.0 80-84 36.171 37.0 37.0 37.0 37.0 37.0 85-89 36.126799999999996 37.0 37.0 37.0 37.0 37.0 90-94 36.1101 37.0 37.0 37.0 37.0 37.0 95-99 36.0963 37.0 37.0 37.0 37.0 37.0 100-104 36.0466 37.0 37.0 37.0 37.0 37.0 105-109 36.087900000000005 37.0 37.0 37.0 37.0 37.0 110-114 36.018100000000004 37.0 37.0 37.0 37.0 37.0 115-119 36.0212 37.0 37.0 37.0 37.0 37.0 120-124 35.9053 37.0 37.0 37.0 37.0 37.0 125-129 35.947199999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.8927 37.0 37.0 37.0 37.0 37.0 135-139 35.826800000000006 37.0 37.0 37.0 37.0 37.0 140-144 35.7322 37.0 37.0 37.0 37.0 37.0 145-149 35.5219 37.0 37.0 37.0 37.0 37.0 150-151 35.335499999999996 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 1.0 23 0.0 24 2.0 25 2.0 26 7.0 27 9.0 28 13.0 29 25.0 30 25.0 31 51.0 32 48.0 33 72.0 34 130.0 35 319.0 36 2941.0 37 353.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.75 11.1 10.299999999999999 45.85 2 20.401002506265662 13.609022556390975 36.99248120300752 28.997493734335837 3 16.175 17.424999999999997 27.1 39.300000000000004 4 20.674999999999997 24.85 24.425 30.049999999999997 5 23.9 31.15 23.325000000000003 21.625 6 20.65 35.425000000000004 24.5 19.425 7 14.875 28.375 38.975 17.775 8 17.8 26.224999999999998 31.474999999999998 24.5 9 16.7 25.924999999999997 36.05 21.325 10-14 19.595000000000002 29.12 28.689999999999998 22.595000000000002 15-19 19.375 28.64 27.52 24.465 20-24 20.525 28.189999999999998 28.199999999999996 23.085 25-29 19.314999999999998 28.285 27.82 24.58 30-34 19.93 28.725 27.189999999999998 24.154999999999998 35-39 19.134999999999998 28.845 27.915 24.104999999999997 40-44 20.325 27.589999999999996 28.13 23.955000000000002 45-49 19.49 28.000000000000004 28.165000000000003 24.345 50-54 19.675 29.075 27.310000000000002 23.94 55-59 19.855 28.055000000000003 27.825 24.265 60-64 19.814999999999998 28.42 28.075 23.69 65-69 20.78 28.444999999999997 27.54 23.235 70-74 20.06 28.249999999999996 27.425 24.265 75-79 19.985 28.43 28.04 23.544999999999998 80-84 20.44 28.675 26.700000000000003 24.185000000000002 85-89 20.13 28.725 27.16 23.985 90-94 20.645 28.549999999999997 27.855 22.95 95-99 20.525 28.405 27.72 23.35 100-104 20.53 28.110000000000003 27.525 23.835 105-109 20.57 29.115000000000002 27.224999999999998 23.09 110-114 20.45 28.244999999999997 28.03 23.275000000000002 115-119 20.805 28.945 27.045 23.205000000000002 120-124 21.07 28.025 27.275 23.630000000000003 125-129 20.630000000000003 27.815 27.325 24.23 130-134 20.66 28.415000000000003 27.150000000000002 23.775 135-139 21.25 29.054999999999996 26.02 23.674999999999997 140-144 21.195 28.38 26.995 23.43 145-149 21.0 28.939999999999998 26.235000000000003 23.825 150-151 21.45 28.225 26.400000000000002 23.925 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 2.5 21 3.5 22 1.0 23 1.5 24 6.0 25 5.5 26 1.5 27 2.5 28 9.0 29 15.5 30 20.0 31 27.0 32 36.0 33 48.5 34 51.0 35 53.5 36 87.5 37 119.0 38 146.0 39 183.0 40 200.5 41 208.5 42 211.0 43 257.5 44 264.5 45 240.5 46 247.5 47 240.5 48 235.5 49 214.0 50 172.5 51 132.5 52 121.0 53 99.5 54 71.0 55 56.0 56 47.5 57 54.0 58 48.0 59 24.0 60 10.5 61 6.5 62 3.5 63 4.5 64 4.5 65 1.0 66 1.0 67 1.0 68 0.5 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.25 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 68.58108108108108 45.675 2 19.81981981981982 26.400000000000002 3 7.31981981981982 14.625 4 2.6276276276276276 7.000000000000001 5 0.9009009009009009 3.0 6 0.4129129129129129 1.6500000000000001 7 0.2627627627627628 1.225 8 0.03753753753753754 0.2 9 0.03753753753753754 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 9 0.22499999999999998 No Hit CACATTGCGATTTGCACGTTCGTCTTTAGTGAATATATAATGATCTGGTA 8 0.2 No Hit GCCATTATCAACACCTTCACTTTCAACCTGATCTTCTTCTCCACTTTCAA 7 0.17500000000000002 No Hit GTCCATATCCATTAGGTGAAAACAAGTTTAAGCAGTAAATATAGTCCATA 7 0.17500000000000002 No Hit GCCACAAACTTGATCCCCATAGAGAACATTCATTGTCGTGCCAAAACCAG 7 0.17500000000000002 No Hit ACTTGGTATGTGCATGTATTGATTAAGCCTTTCAACAGAAATGATGTAAT 7 0.17500000000000002 No Hit GACCGATCTAGGGATGAGAACGTCCCCGATGTTGTTGTGCCATGGGTCAG 7 0.17500000000000002 No Hit TGTATACGCCACACATCCACCATGCAAAAAGTAAACCCTCACAAATGAGA 7 0.17500000000000002 No Hit TCCAGCAGTAATTGCTAAATGTCTATAGGAAGGGACCACTCCCTTACTCC 7 0.17500000000000002 No Hit GTCTCACAATCTCAAACTTGGGCTTGTATGTCAAATCAACAACTTGCTCC 6 0.15 No Hit CTTTCTCAATCTCTGCAATTTCTTCCCTAATGGAGATAAGCGCATCACAG 6 0.15 No Hit ATTCCTTCATACCTCCACCTGCTGCACTGGGATAATATATCATGATGCTG 6 0.15 No Hit AGGAAATTTTGGGACCCCATTTGAAGATAAAGAAAACCCGGTAAAAGTTT 6 0.15 No Hit CGTGATTCGACACAATAATCCCTGCTGCTCCAGCTTGAACTGAAAGCCTT 6 0.15 No Hit TAGGTGTGGTAGTTTTAAGGGAGTGATTCTTGCTGAAGGTGGCAAAGCAA 6 0.15 No Hit GTTCCTATTTGTTGTCGAGACGCAGACCATAGGCTCATTCATGCGAGCAT 6 0.15 No Hit CCGGACAAGAGGCCTGCATACATGCTCGAGCTGCCATTGTCTTGTCCTAT 6 0.15 No Hit CCCCACATATCAGCATTCTATACAAAACAAAAGGAGTTTCATACTACAAA 6 0.15 No Hit CAAGGATGAATCCCAACGCCGTTACTCTTAGCAACCTCCTTTTCTACTTC 6 0.15 No Hit CACCATATTTGTCGACATATTGGTACACACCCTTTCCCTTGGGCTTCCTT 6 0.15 No Hit CACAGACAGATGAAAAGAAAAGAGGCAGCACTGCTGTGTATATATGTGAG 5 0.125 No Hit CTCGAATCAATTACAAAAGTTGGAACAGCCAGATATTTAAAGTAGACTTT 5 0.125 No Hit CTGGTTGAAAACACCTGTTGGTAGAGCAGAAGTGGCAACTTCCAGCATAT 5 0.125 No Hit GTCCTCATTTTTTCTTCCTCATTCCCTGGATACTCTCTAATCATAAAAAG 5 0.125 No Hit GTTTAGTTAATGTCAATGGTAAATCATGCATTTAATCCAAATGAACAGAG 5 0.125 No Hit CCAACAAAAACTCCGACATGCTCGGCGGCAACGGCCTCAACCCGGACCCA 5 0.125 No Hit CTTAATGACATTGGCAATACTAATACAAAACAGTAGATGAGTACATTTTA 5 0.125 No Hit GTCAGTTCTGAAGTAGCCACCACTCTTCGAAATTGGAGCAGCTCGCCAGT 5 0.125 No Hit GTTCCAAGGTTGTAGAACTTCAAATAGTCCTTCCAGTCAAAGAAGACTCT 5 0.125 No Hit CTTTCAAACTACGTACCATACAGATTATGCACTTTCAGCACATATGATTA 5 0.125 No Hit GTTTGGTGATCTTGAGGATTTTAGGGAAGGAAAGTTGTGGGATTTGGAGT 5 0.125 No Hit CTCCTTGTTGTTGTTGTTGTTGTTATCAACTGTTTCGTCCCCCTTTCCCT 5 0.125 No Hit CTCCTAAATGGATCTCTGAAAATAGCTTGTGGATATAGGATCACTTCACT 5 0.125 No Hit GAAGATAAGAAAGCAGCGGATAGAACTGCACCAGAAAGAAAATCCAGTAC 5 0.125 No Hit TCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAG 5 0.125 No Hit ACCGCCTGTTGCCACCCGTTCAGTTGGATTCTTCCTCCTCCTATCATTCC 5 0.125 No Hit GAGCTGGTGGGATACCATCTAGGCGGAAGCTGCCAAGAGATTTGTTGTCC 5 0.125 No Hit AAATAGCCAAGAAAACCAGAGTAACCCTTTCGAGTAGTCTCCCCACTCCA 5 0.125 No Hit GCCTGCTTCTGTGCCCTGCAAACCAGCTGGGCGGGCTTGAGGTTAACCAC 5 0.125 No Hit CCCCTTTACCACTCCAATGCAAGAGACTAACAGGACCAGGGTGCAAATCT 5 0.125 No Hit GGACAGGGAACAAAGTTTTGGATGTCAGCAGCAATCCAATTTCAGCTCTG 5 0.125 No Hit TACCACACTGCGGACACTCCTCTGGCCCCATTCCTCCACTTCCATTGCTG 5 0.125 No Hit GTCATCGTAATCTTCTGCCCCAATACCACACTCTAGTCCAGCATTTACCT 5 0.125 No Hit CTTGATAGTAACAGAAACAGACCAAAATCTAGGATACAAGGAAAACAAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.23750000000000002 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.36250000000000004 0.0 0.0 0.0 0.0 84-85 0.44999999999999996 0.0 0.0 0.0 0.0 86-87 0.5375000000000001 0.0 0.0 0.0 0.0 88-89 0.75 0.0 0.0 0.0 0.0 90-91 0.9 0.0 0.0 0.0 0.0 92-93 1.0875 0.0 0.0 0.0 0.0 94-95 1.2125 0.0 0.0 0.0 0.0 96-97 1.4 0.0 0.0 0.0 0.0 98-99 1.625 0.0 0.0 0.0 0.0 100-101 1.7125 0.0 0.0 0.0 0.0 102-103 2.1375 0.0 0.0 0.0 0.0 104-105 2.5 0.0 0.0 0.0 0.0 106-107 3.25 0.0 0.0 0.0 0.0 108-109 3.475 0.0 0.0 0.0 0.0 110-111 3.8625 0.0 0.0 0.0 0.0 112-113 4.262499999999999 0.0 0.0 0.0 0.0 114-115 4.5875 0.0 0.0 0.0 0.0 116-117 4.9125 0.0 0.0 0.0 0.0 118-119 5.3875 0.0 0.0 0.0 0.0 120-121 6.0375 0.0 0.0 0.0 0.0 122-123 6.45 0.0 0.0 0.0 0.0 124-125 6.9375 0.0 0.0 0.0 0.0 126-127 7.325 0.0 0.0 0.0 0.0 128-129 8.075 0.0 0.0 0.0 0.0 130-131 8.8125 0.0 0.0 0.0 0.0 132-133 9.425 0.0 0.0 0.0 0.0 134-135 9.7625 0.0 0.0 0.0 0.0 136-137 10.325 0.0 0.0 0.0 0.0 138-139 10.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATCCA 10 0.006830828 145.0 8 >>END_MODULE SRR13695416 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695416_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.31975 37.0 37.0 37.0 37.0 37.0 2 36.144 37.0 37.0 37.0 37.0 37.0 3 36.1165 37.0 37.0 37.0 37.0 37.0 4 36.192 37.0 37.0 37.0 37.0 37.0 5 36.2875 37.0 37.0 37.0 37.0 37.0 6 36.2735 37.0 37.0 37.0 37.0 37.0 7 36.233 37.0 37.0 37.0 37.0 37.0 8 36.2505 37.0 37.0 37.0 37.0 37.0 9 36.3145 37.0 37.0 37.0 37.0 37.0 10-14 36.272800000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.26270000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.20465 37.0 37.0 37.0 37.0 37.0 25-29 36.1908 37.0 37.0 37.0 37.0 37.0 30-34 36.1252 37.0 37.0 37.0 37.0 37.0 35-39 36.170100000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.1879 37.0 37.0 37.0 37.0 37.0 45-49 36.16205 37.0 37.0 37.0 37.0 37.0 50-54 36.069399999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.0947 37.0 37.0 37.0 37.0 37.0 60-64 35.948899999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.9665 37.0 37.0 37.0 37.0 37.0 70-74 35.9875 37.0 37.0 37.0 37.0 37.0 75-79 35.93215 37.0 37.0 37.0 37.0 37.0 80-84 35.98285 37.0 37.0 37.0 37.0 37.0 85-89 35.891400000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.85845 37.0 37.0 37.0 37.0 37.0 95-99 35.8787 37.0 37.0 37.0 37.0 37.0 100-104 35.85260000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.8078 37.0 37.0 37.0 37.0 37.0 110-114 35.693 37.0 37.0 37.0 37.0 37.0 115-119 35.6841 37.0 37.0 37.0 37.0 37.0 120-124 35.608000000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.60334999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.4045 37.0 37.0 37.0 37.0 37.0 135-139 35.314099999999996 37.0 37.0 37.0 32.2 37.0 140-144 35.1935 37.0 37.0 37.0 29.8 37.0 145-149 35.007000000000005 37.0 37.0 37.0 29.8 37.0 150-151 34.689750000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.0 14 2.0 15 2.0 16 2.0 17 1.0 18 0.0 19 0.0 20 1.0 21 3.0 22 3.0 23 6.0 24 4.0 25 4.0 26 6.0 27 8.0 28 13.0 29 15.0 30 27.0 31 42.0 32 62.0 33 112.0 34 232.0 35 609.0 36 2613.0 37 230.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.78848560700876 21.65206508135169 14.117647058823529 33.441802252816025 2 26.35 26.05 31.525 16.075 3 21.099999999999998 28.9 28.95 21.05 4 23.974999999999998 33.025 23.974999999999998 19.025 5 24.425 36.3 23.075000000000003 16.2 6 20.125 36.9 24.8 18.175 7 18.075 21.775 40.575 19.575 8 20.150000000000002 24.85 29.849999999999998 25.15 9 21.3 24.125 30.65 23.925 10-14 22.695 30.04 26.674999999999997 20.59 15-19 23.055 27.529999999999998 28.605000000000004 20.810000000000002 20-24 22.55563890972743 28.962240560140035 27.7569392348087 20.72518129532383 25-29 22.42121060530265 28.67933966983492 27.528764382191095 21.370685342671337 30-34 23.269307723089234 28.456382553021207 27.81112444977991 20.463185274109644 35-39 23.086926077823346 28.433530059017702 27.98839651895569 20.491147344203263 40-44 22.904161664665867 27.03581432573029 28.761504601840738 21.298519407763106 45-49 22.397839243735305 28.094833191617063 28.775071274946228 20.732256289701397 50-54 23.056917075122538 28.6185855756727 27.993398019405824 20.331099329798942 55-59 23.656828414207105 27.898949474737368 27.25862931465733 21.1855927963982 60-64 22.646794038211464 27.89336801040312 28.573572071621488 20.88626587976393 65-69 24.027402740274027 27.2977297729773 27.86278627862786 20.812081208120812 70-74 22.7663831915958 28.114057028514257 27.99899949974988 21.12056028014007 75-79 23.925766594967733 28.267720474213398 27.507378320244108 20.29913461057476 80-84 23.37584396099025 27.796949237309327 27.82195548887222 21.005251312828207 85-89 23.74687343671836 28.31415707853927 27.303651825912954 20.635317658829415 90-94 24.00840294102936 27.03946381233432 28.13484719651878 20.81728605011754 95-99 23.46704011203361 28.64359307792338 26.993097929378813 20.8962688806642 100-104 24.06703351675838 28.38419209604802 26.863431715857928 20.68534267133567 105-109 22.81640820410205 28.104052026013004 27.973986993496748 21.105552776388194 110-114 24.187256176853055 29.39881964589377 26.84305291587476 19.570871261378414 115-119 24.072036018009005 28.4392196098049 27.078539269634817 20.410205102551277 120-124 23.871935967983994 28.254127063531765 28.024012006003 19.849924962481243 125-129 25.78160172077435 27.68745935671052 26.44690110549747 20.08403781701766 130-134 25.012506253126567 28.61430715357679 26.52826413206603 19.844922461230617 135-139 25.6128064032016 28.064032016008007 27.288644322161083 19.034517258629315 140-144 26.102830849254776 27.8333500050015 26.217865359607885 19.84595378613584 145-149 26.59829914957479 27.20360180090045 26.918459229614804 19.279639819909956 150-151 27.176088044022013 28.23911955977989 25.78789394697349 18.79689844922461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 0.5 11 1.0 12 3.0 13 2.0 14 0.5 15 0.5 16 0.0 17 0.5 18 1.0 19 1.5 20 1.5 21 1.5 22 1.5 23 1.0 24 2.0 25 7.5 26 7.5 27 4.5 28 8.5 29 12.5 30 17.5 31 23.5 32 29.5 33 48.5 34 64.5 35 75.0 36 86.5 37 108.5 38 124.5 39 144.5 40 176.5 41 224.0 42 258.0 43 265.5 44 266.0 45 262.5 46 268.5 47 260.0 48 246.0 49 201.5 50 150.0 51 134.0 52 114.5 53 97.5 54 78.0 55 46.0 56 37.0 57 35.0 58 27.0 59 14.5 60 7.5 61 9.5 62 7.5 63 9.0 64 9.0 65 4.5 66 2.5 67 0.0 68 0.5 69 0.5 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.05 30-34 0.04 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.01 70-74 0.05 75-79 0.045 80-84 0.025 85-89 0.05 90-94 0.034999999999999996 95-99 0.03 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.64999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 68.67966991747937 45.775 2 19.42985746436609 25.900000000000002 3 7.689422355588897 15.375 4 2.550637659414854 6.800000000000001 5 0.9377344336084021 3.125 6 0.48762190547636913 1.95 7 0.1875468867216804 0.8750000000000001 8 0.037509377344336084 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATGTTTGTGGCCCTGGTTCTATTGGGATCTTCAAGAAAGAGTTTGGAC 8 0.2 No Hit CTGGGATTTTTGGATGTGCTGATGATACCGAAAGCTGCTGGACAGGACTA 7 0.17500000000000002 No Hit GTCTGTTTGGATATTTGCAGAACCCAGCTATAAGATATTTGATTATGCAA 7 0.17500000000000002 No Hit AGAAGAACAATAAGGCATCTCAAAATACCAGAAGTAATCTTGTATTTTTT 7 0.17500000000000002 No Hit GCCTTTCTTGGCAAATGGAAGCCCACCAAGAATGATACCCTTGCTAAGCG 7 0.17500000000000002 No Hit GCAAATGAGTGGTTGATTCAACGGCTTGAAATATTTAGTGCAACTGTTCT 7 0.17500000000000002 No Hit CAGCACATACACCAGTACTCTTGCTTGCTCTTTGGGTAGGGGGCACCAGC 6 0.15 No Hit GAGAGACATGGAAGCGGTGCTCCAAACCAAAGGGCTTCTTTCACTACCTT 6 0.15 No Hit TTCAGCTCTAGAGGACAAGGATAATATCCAATCTTCAGTTGACCTAACCG 6 0.15 No Hit CCAGGATTCGACGGCCGAAGACACTCCCAGACTTGCTCGCCGGAAGGAAT 6 0.15 No Hit TAAGAAGGTAAACTCATATCCAACAGTTAGAGGATTTACTGCAATAGGTA 6 0.15 No Hit AGATGTTGCCAAGCGTCTTATGGACTATGGATTTCATGCACCTACAATGT 6 0.15 No Hit CACAACTCTAAATTTTGCATCTTTAGTCATCAGCCATGGCCAACATGATC 6 0.15 No Hit GAACTTTCAAGCAGCCATTGGGAAAGAAATTGTTGATGTTACTCTGATTG 6 0.15 No Hit AAGACTTGCGCCCAGATTTTTGAAGAAGCTGGGTGTGACACGCCTGCAAG 6 0.15 No Hit CTGGTGATGATGCAGCTACCAACATTTCTCCGGCTACTCCTCCCCTTTTC 6 0.15 No Hit GCCTTTTCTTGTGAATGAATTGGTGCCACAACATCTTCTTCATGACCGGC 6 0.15 No Hit GTCAAGAAGATCAAGACCGATACGCCTTATGGAACTGGTGGTGGCATGAA 6 0.15 No Hit CAAGGCTGCTGACTCTGGACTTGCGTCGTATGTTGCTGGTCAAATTGATC 6 0.15 No Hit CCGAGGTGGTGGTGGGTTTGAGCTTTATTACGATGACGGTGGTGGGTCCG 5 0.125 No Hit AAATGTTTCAAAAGATATTGGATGAAGCATTGGCAGGTGATAATGTTGGG 5 0.125 No Hit GAAGTTGTTGCCCTTGGTGCTGCAGTTCAGGCTGGTGTCTTGGCTGGAGA 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit AGGTTGTTCTTGAATGAAAGAAAAACTTGGGTCCATCTATTAGCACTTTA 5 0.125 No Hit TGTATATAATACTACTAGTTATAGAGAGTGAGTGACAACTAGTAGCTAGC 5 0.125 No Hit CCAGAGTTCACTCTTATGGAATGAAGGTGATGTTGACCCTGTTCCATCAC 5 0.125 No Hit CTTCATCCAAGTGGACCACTAACTTTCTTAGTGCTGCTTCAACTGTTCGA 5 0.125 No Hit CCAACGGAGCTGGAAAGAGTACCTTGCTGAAGCTTATGACGGGTGACTTG 5 0.125 No Hit CATTAACCTTAACCTCTCAGACTCCACTGAGAAAATCATTGCTGAGTACT 5 0.125 No Hit CAATCACCAAAACTCGAACTGAAATTGGATCCAGGAGATTGAAACCTTCA 5 0.125 No Hit TGGAACTACAGAAGAGAATGAGAATTTACGAGTTGGGTTCATTGCCACCA 5 0.125 No Hit TTTTGAATATCAAACTTCTCGCTGAAAAAGGCTCCTCTTTCTTTCAGCAG 5 0.125 No Hit GAATAAGCCTCCAGTTGTCAGTGTGCTGAAAAGATTGTGTATGTAGCCAT 5 0.125 No Hit TATGTTTTTAAGTAGTTGTGTGTATGTTCTGTGGTATTCCATGCTATGTG 5 0.125 No Hit GTTTTTAATAATGGAAATGGGATACTTTTGTCTAATATGGTAACATGAAG 5 0.125 No Hit CACGTTTTCCATCTGCTGTTTATCTAACCTTGTACAAATGATATCTTTTC 5 0.125 No Hit CACTACCTGAAGATGATGAACAACAACAACATCAACAAAATCAAAAGCCA 5 0.125 No Hit GTCAACAACAACGCCTGGGCATATGCCACCAACTTCGCCCCCGGAAAGTG 5 0.125 No Hit CTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACT 5 0.125 No Hit CTGGGGAATGTAGAATGTATATATGATGGAGTGGGGAGACTACTCGAAAG 5 0.125 No Hit CTCCAAGGACCCTCAGAAGTTCGAGGAATACAAAGTCAAGGAAATTAAGA 5 0.125 No Hit ATTTGTGCCCTTGATTTCCGTATGGATGAATGGGGAGTAGATGTGGCTTT 5 0.125 No Hit TGCTTGTGAAACAAGTAAAGATGAAAATGGAAAGAACAAGATTTTAGGAA 5 0.125 No Hit AGAGGCCTAAATCTCTGGGCTGTTCGGACCACCGAGGTTATTCAAACGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.23750000000000002 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.36250000000000004 0.0 0.0 0.0 0.0 84-85 0.44999999999999996 0.0 0.0 0.0 0.0 86-87 0.5375000000000001 0.0 0.0 0.0 0.0 88-89 0.75 0.0 0.0 0.0 0.0 90-91 0.9 0.0 0.0 0.0 0.0 92-93 1.0875 0.0 0.0 0.0 0.0 94-95 1.2125 0.0 0.0 0.0 0.0 96-97 1.4 0.0 0.0 0.0 0.0 98-99 1.625 0.0 0.0 0.0 0.0 100-101 1.7375 0.0 0.0 0.0 0.0 102-103 2.1625 0.0 0.0 0.0 0.0 104-105 2.5250000000000004 0.0 0.0 0.0 0.0 106-107 3.2625 0.0 0.0 0.0 0.0 108-109 3.475 0.0 0.0 0.0 0.0 110-111 3.8625 0.0 0.0 0.0 0.0 112-113 4.262499999999999 0.0 0.0 0.0 0.0 114-115 4.5875 0.0 0.0 0.0 0.0 116-117 4.9 0.0 0.0 0.0 0.0 118-119 5.3625 0.0 0.0 0.0 0.0 120-121 6.0125 0.0 0.0 0.0 0.0 122-123 6.4 0.0 0.0 0.0 0.0 124-125 6.8875 0.0 0.0 0.0 0.0 126-127 7.275 0.0 0.0 0.0 0.0 128-129 8.0625 0.0 0.0 0.0 0.0 130-131 8.8125 0.0 0.0 0.0 0.0 132-133 9.45 0.0 0.0 0.0 0.0 134-135 9.8125 0.0 0.0 0.0 0.0 136-137 10.375 0.0 0.0 0.0 0.0 138-139 11.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194074 spots for SRR13695416.sra Written 194074 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra Read 194062 spots for SRR13695416.sra Written 194062 spots for SRR13695416.sra SRR ids: ['SRR13695416.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_txk9cs6b SRR13695416.sra spots: 3881252 blocks: [[1, 194062], [194063, 388124], [388125, 582186], [582187, 776248], [776249, 970310], [970311, 1164372], [1164373, 1358434], [1358435, 1552496], [1552497, 1746558], [1746559, 1940620], [1940621, 2134682], [2134683, 2328744], [2328745, 2522806], [2522807, 2716868], [2716869, 2910930], [2910931, 3104992], [3104993, 3299054], [3299055, 3493116], [3493117, 3687178], [3687179, 3881252]] SRR13695416 file size 1309269 SRR13695416 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695416 SRR13695416_1.fastq SRR13695416_2.fastq Input file: SRR13695416_1.fastq Paired file: SRR13695416_2.fastq trimmed: SRR13695416-trimmed-pair1.fastq, SRR13695416-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:28:39 2025 >> started Wed Feb 12 01:28:43 2025 >> done (4.447s) 3881252 read pairs processed; of these: 28 ( 0.00%) short read pairs filtered out after trimming by size control 566 ( 0.01%) empty read pairs filtered out after trimming by size control 3880658 (99.98%) read pairs available; of these: 601621 (15.50%) trimmed read pairs available after processing 3279037 (84.50%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 26 1 0.00% 27 1 0.00% 28 3 0.00% 29 1 0.00% 30 2 0.00% 31 0 0.00% 32 1 0.00% 33 1 0.00% 34 4 0.00% 35 3 0.00% 36 6 0.00% 37 4 0.00% 38 2 0.00% 39 3 0.00% 40 13 0.00% 41 5 0.00% 42 7 0.00% 43 9 0.00% 44 4 0.00% 45 6 0.00% 46 6 0.00% 47 20 0.00% 48 17 0.00% 49 14 0.00% 50 18 0.00% 51 17 0.00% 52 37 0.00% 53 29 0.00% 54 31 0.00% 55 32 0.00% 56 45 0.00% 57 60 0.00% 58 79 0.00% 59 70 0.00% 60 63 0.00% 61 113 0.00% 62 129 0.00% 63 115 0.00% 64 142 0.00% 65 157 0.00% 66 167 0.00% 67 195 0.01% 68 238 0.01% 69 241 0.01% 70 340 0.01% 71 335 0.01% 72 425 0.01% 73 483 0.01% 74 495 0.01% 75 630 0.02% 76 715 0.02% 77 690 0.02% 78 868 0.02% 79 953 0.02% 80 1052 0.03% 81 1084 0.03% 82 1293 0.03% 83 1483 0.04% 84 1543 0.04% 85 1861 0.05% 86 1933 0.05% 87 2087 0.05% 88 2274 0.06% 89 2433 0.06% 90 2667 0.07% 91 2857 0.07% 92 2969 0.08% 93 3242 0.08% 94 3563 0.09% 95 3680 0.09% 96 3990 0.10% 97 4303 0.11% 98 4438 0.11% 99 4407 0.11% 100 4622 0.12% 101 4807 0.12% 102 5143 0.13% 103 5409 0.14% 104 5752 0.15% 105 6184 0.16% 106 6339 0.16% 107 6513 0.17% 108 6843 0.18% 109 6981 0.18% 110 7334 0.19% 111 7674 0.20% 112 7474 0.19% 113 7808 0.20% 114 8212 0.21% 115 8372 0.22% 116 8822 0.23% 117 8986 0.23% 118 9229 0.24% 119 9220 0.24% 120 9555 0.25% 121 9854 0.25% 122 9898 0.26% 123 10183 0.26% 124 10746 0.28% 125 10796 0.28% 126 11229 0.29% 127 11721 0.30% 128 11744 0.30% 129 11728 0.30% 130 12175 0.31% 131 12054 0.31% 132 12210 0.31% 133 12546 0.32% 134 12613 0.33% 135 12826 0.33% 136 13240 0.34% 137 13426 0.35% 138 13580 0.35% 139 13747 0.35% 140 14177 0.37% 141 14395 0.37% 142 14416 0.37% 143 14553 0.38% 144 14614 0.38% 145 14335 0.37% 146 14967 0.39% 147 14919 0.38% 148 15215 0.39% 149 15449 0.40% 150 15782 0.41% 151 3279037 84.50% 3880658 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=28 prefix-density=0.30 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=281.40 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=16.9 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGT criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=30 prefix-density=0.36 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.12 sequence-density-rank=26 fanout-score=37.84 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=12.8 sequence=AAAGAAAAGAAAA SRR13695416 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:29:25 Started mapping on | Feb 12 01:29:25 Finished on | Feb 12 01:29:57 Mapping speed, Million of reads per hour | 436.57 Number of input reads | 3880658 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 3666198 Uniquely mapped reads % | 94.47% Average mapped length | 292.66 Number of splices: Total | 3511695 Number of splices: Annotated (sjdb) | 3431189 Number of splices: GT/AG | 3441519 Number of splices: GC/AG | 54180 Number of splices: AT/AC | 2263 Number of splices: Non-canonical | 13733 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.96 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 89288 % of reads mapped to multiple loci | 2.30% Number of reads mapped to too many loci | 12183 % of reads mapped to too many loci | 0.31% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.81% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 125218 125218 125218 N_multimapping 89288 89288 89288 N_noFeature 145313 3577012 196813 N_ambiguous 62777 396 24824 UnstrandedReadsAssigned:3458108 PositiveStrandReadsAssigned:88790 NegativeStrandReadsAssigned:3444561 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695416 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695416-trimmed-pair1.fastq SRR13695416-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,880,658 reads, 3,447,970 reads pseudoaligned [quant] estimated average fragment length: 237.52 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,010 rounds 52401 SRR13695416.ke.tsv 34699 SRR13695416.se.tsv 87100 total ==> SRR13695416.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1781.48 167 24.4475 Potri.005G024800.1.v4.1 1035 798.48 55 17.9637 Potri.004G059700.1.v4.1 961 724.518 0 0 Potri.007G009000.2.v4.1 1416 1179.48 0 0 Potri.003G141000.2.v4.1 2943 2706.48 155 14.9357 Potri.016G087400.1.v4.1 270 90.5478 194 558.756 Potri.015G069301.1.v4.1 564 335.662 0 0 Potri.010G195200.1.v4.1 1773 1536.48 10 1.69735 Potri.012G127500.1.v4.1 977 740.491 88 30.9928 ==> SRR13695416.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 100 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 59 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR13695416 completed mapping pipeline successfully