Starting /dee2/code/volunteer_pipeline.sh SRR13695417 current disk space = 3051199864832 free memory = 1028586580 SRR13695417 SRAfilesize 9350af454309fea486de68fc0369e251 SRR13695417.sra SRR13695417.sra file validated SRR13695417 is paired end SRR13695417 is conventional basespace SRR13695417 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695417_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5535 37.0 37.0 37.0 37.0 37.0 2 36.357 37.0 37.0 37.0 37.0 37.0 3 36.5565 37.0 37.0 37.0 37.0 37.0 4 36.619 37.0 37.0 37.0 37.0 37.0 5 36.627 37.0 37.0 37.0 37.0 37.0 6 36.571 37.0 37.0 37.0 37.0 37.0 7 36.4865 37.0 37.0 37.0 37.0 37.0 8 36.557 37.0 37.0 37.0 37.0 37.0 9 36.564 37.0 37.0 37.0 37.0 37.0 10-14 36.569 37.0 37.0 37.0 37.0 37.0 15-19 36.5279 37.0 37.0 37.0 37.0 37.0 20-24 36.531000000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.470299999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.457800000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.4556 37.0 37.0 37.0 37.0 37.0 40-44 36.4245 37.0 37.0 37.0 37.0 37.0 45-49 36.39059999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.3831 37.0 37.0 37.0 37.0 37.0 55-59 36.389900000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.3794 37.0 37.0 37.0 37.0 37.0 65-69 36.2993 37.0 37.0 37.0 37.0 37.0 70-74 36.3013 37.0 37.0 37.0 37.0 37.0 75-79 36.3039 37.0 37.0 37.0 37.0 37.0 80-84 36.250099999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.2255 37.0 37.0 37.0 37.0 37.0 90-94 36.1443 37.0 37.0 37.0 37.0 37.0 95-99 36.091699999999996 37.0 37.0 37.0 37.0 37.0 100-104 36.0644 37.0 37.0 37.0 37.0 37.0 105-109 36.12180000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.0989 37.0 37.0 37.0 37.0 37.0 115-119 36.1008 37.0 37.0 37.0 37.0 37.0 120-124 36.0503 37.0 37.0 37.0 37.0 37.0 125-129 35.9699 37.0 37.0 37.0 37.0 37.0 130-134 35.9381 37.0 37.0 37.0 37.0 37.0 135-139 35.8831 37.0 37.0 37.0 37.0 37.0 140-144 35.7966 37.0 37.0 37.0 37.0 37.0 145-149 35.6338 37.0 37.0 37.0 37.0 37.0 150-151 35.445499999999996 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 0.0 23 1.0 24 1.0 25 2.0 26 6.0 27 8.0 28 20.0 29 16.0 30 28.0 31 42.0 32 46.0 33 62.0 34 109.0 35 291.0 36 3030.0 37 335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.875 11.625 6.45 45.050000000000004 2 18.23204419889503 14.61577096936213 38.548468106479156 28.603716725263688 3 18.15 16.225 27.474999999999998 38.15 4 22.8 25.25 23.599999999999998 28.349999999999998 5 21.875 32.775 26.275 19.075 6 19.35 35.575 23.825 21.25 7 14.499999999999998 26.275 42.199999999999996 17.025000000000002 8 16.625 25.55 33.650000000000006 24.175 9 16.75 24.6 35.099999999999994 23.549999999999997 10-14 19.15 29.835 28.175 22.84 15-19 19.634999999999998 27.76 28.625 23.98 20-24 19.189999999999998 29.325000000000003 27.16 24.325 25-29 19.994999999999997 28.134999999999998 28.115000000000002 23.755000000000003 30-34 19.77 27.515 27.775 24.94 35-39 19.11 29.104999999999997 28.610000000000003 23.175 40-44 20.165 28.765 27.71 23.36 45-49 19.665 28.689999999999998 27.694999999999997 23.95 50-54 19.59 29.03 29.125 22.255 55-59 19.775000000000002 28.255000000000003 28.42 23.549999999999997 60-64 19.775000000000002 27.76 28.84 23.625 65-69 19.855 28.895 27.839999999999996 23.41 70-74 20.505000000000003 27.82 28.29 23.385 75-79 19.86 29.13 27.24 23.77 80-84 21.085 28.645 27.54 22.73 85-89 19.33 29.175 28.15 23.345 90-94 20.135 28.21 27.83 23.825 95-99 20.285 28.92 27.944999999999997 22.85 100-104 20.474999999999998 28.985 27.389999999999997 23.150000000000002 105-109 21.695 28.285 27.310000000000002 22.71 110-114 20.25 28.115000000000002 28.060000000000002 23.575 115-119 20.69 29.304999999999996 26.41 23.595 120-124 19.675 28.895 27.450000000000003 23.98 125-129 20.71 28.294999999999998 26.729999999999997 24.265 130-134 20.315 28.144999999999996 27.860000000000003 23.68 135-139 21.154999999999998 27.965 27.060000000000002 23.82 140-144 20.02 28.050000000000004 27.284999999999997 24.645 145-149 20.419999999999998 28.325 27.58 23.674999999999997 150-151 20.9125 27.675 25.112499999999997 26.3 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 2.0 21 1.0 22 0.0 23 0.0 24 2.0 25 4.0 26 5.0 27 5.5 28 11.5 29 21.5 30 25.5 31 30.0 32 36.0 33 47.0 34 60.5 35 66.0 36 87.0 37 115.0 38 136.0 39 171.5 40 205.5 41 230.0 42 273.0 43 271.0 44 230.5 45 282.5 46 275.5 47 231.5 48 223.0 49 178.0 50 161.5 51 138.0 52 104.0 53 85.5 54 66.5 55 41.0 56 35.5 57 32.5 58 24.0 59 24.5 60 17.5 61 11.5 62 9.5 63 4.0 64 2.5 65 4.0 66 2.5 67 0.0 68 0.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 68.76876876876878 45.800000000000004 2 19.85735735735736 26.450000000000003 3 7.057057057057057 14.099999999999998 4 2.5525525525525525 6.800000000000001 5 0.7507507507507507 2.5 6 0.6756756756756757 2.7 7 0.22522522522522523 1.05 8 0.11261261261261261 0.6 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTGTATCCCTAATTGAAGCTGCAGCCTTCTCATCCTTTGCAACCAGGAT 8 0.2 No Hit CAGATGGGATCACATTCTGAACCTCTGGTGCCAGAGATGCAGCTGTGAAA 8 0.2 No Hit CACTGCTTCAAGCTTCCGAGCAATCTCATCAACTCTCTGTTGGTCTTCAG 8 0.2 No Hit TGGAAGCTCAAAATCTCACGAACTGCAGAGCTTTGATACACATCAAGTTT 7 0.17500000000000002 No Hit AAATAATAGAGATTTAAATAGGAGAAATCATCCAAAAACAGCAGAAAAGT 7 0.17500000000000002 No Hit CACATGTGTCTAAAGAAGCGCTGATGGCTTGACCAATATACTCTCCACAA 7 0.17500000000000002 No Hit ATTGCTACAACTGGTGCTTCGAGCACCGCCGCATTCTTCGCCATGAATTC 7 0.17500000000000002 No Hit ACCAATTTCAAATGACACACTGGGCATGGAGGATAGACTGCTACATTCAA 7 0.17500000000000002 No Hit GAGCAATCATTTTACATGGTGGACACCATGAAGCAGTAAAATCCACGACA 7 0.17500000000000002 No Hit CCGTGCATGAGGCTCCATCAAGCTCCAGAGACTGCCAGGAATTCCTTCTC 6 0.15 No Hit ATCATTGTTGTCTATGGCTTGCCCTACTTTACTTAGCTTGTACACAAGAT 6 0.15 No Hit GCCCTCTCAAATGCTCATTAAGAATCCCAGAAAAGTCAATCCTTAGGATT 6 0.15 No Hit CTGAGTTTGATTTCCTTCCAACTGCCAGTCCTGGAGATTTGGCAAGTGAA 6 0.15 No Hit CTCAAAACCAACATCAAAGCAACCTTAGCAAACCCATTTTGCGCATACCC 6 0.15 No Hit GCATCTAGAAGCATACAACTTTGTATAAAGCTGATTTTGTTTTCTGTTGA 6 0.15 No Hit CACTTCTGAACTTCCATGCTCCAGTTACTACTGATCTGAGGGTGACTGCT 6 0.15 No Hit CCCATAGGCAACTTCGCACAACAAGGATGAAGATCAAATCCACAGTCTTT 6 0.15 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTATTACCGCGGCTGCT 6 0.15 No Hit GACCCATCGGTTGTCTCTGTATAACGCTCCATGACAGGTTGTACGATCTT 6 0.15 No Hit GTCCCCATGCTATCAAGTTACAATGGAGCTGTGAGCTACATTAGATTTTA 6 0.15 No Hit CAGCTAAACTCTCTCTCATCACTTGCATTGACAGATGCATCAGAATTTCT 6 0.15 No Hit CCCTTATTGTCACTTGCTAATATCTGCATGTATACTTTTGTTTCCTTCGC 6 0.15 No Hit CTCATCTTTCACAAAATGCAGAGCGACTTCTGGGAACCCCTTCTGTTGCA 6 0.15 No Hit CAGGGCTTTTCTCCTGTCATTAGCCTTCGCCTCAGCAATTCTCCTATACA 6 0.15 No Hit GCTCCCTCTGATCCTGGACTTCAAGATTCCACTCTTTCAAGAAGCTATCA 6 0.15 No Hit CTCCAATTCTCTGGCACCAAATTCTCTTCTTCAATTTTAGGAACAACCCT 6 0.15 No Hit CTCCTTAAGAAACTCTTCACCTGATGCCAAATCCAAAGGTATAATCTTTA 6 0.15 No Hit TTGTAATGAGCGTGGAGACTTCGCCTCCTGGCTTGGTAATAAGGCTGCTT 5 0.125 No Hit ACTCAATAATGGAAGACATAAACATTATACTCCACAATAGCATCACCAGT 5 0.125 No Hit CTTGCCTTTGGCTCCAACAAGCTTCTGCCACCCAAATGTTGATCCAGCTT 5 0.125 No Hit GAAGTTAGTAAGGCTCGGGCTCTCCTCCTCCTCCTCAAATAAATGAAGAA 5 0.125 No Hit GGACTGATCTACCCATTCCAGTGCCAACATTGTAGATTCCAACTTTCCCA 5 0.125 No Hit CCACAGACAGGTTTCCCATTATGGACAAGAACTGGGATTTGCTTGTTAAC 5 0.125 No Hit CTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCTCACTC 5 0.125 No Hit CCTGGCATTGCCCATCTGCAGTGAACAAGTTCTGCTTGCACATTCCATCT 5 0.125 No Hit GGGAATGCGTGCTCATGTGAAAGGCAGTAGTTGCAAACTGGTGATGCGCC 5 0.125 No Hit GTGGACAAGTATTCTGGTGCTATGTGGCCCATTGTCCCGCGAACTTGAGT 5 0.125 No Hit CTGGATACTAATTTACATCACCACACACCACTCAAATCACACACACACAA 5 0.125 No Hit GCTCGTAGAACATCCTAACTGGAGCTACACCAGCAATGATTGTCTGACTT 5 0.125 No Hit CCAATGCAAAAAAAGAAAAAAAAAAAGGGCAAAAACTATTCTAGCTATGG 5 0.125 No Hit GTGATGATCAGGTGGAGGTGCACGCGGGCCTGGAGGAGGAGGTTCACGGT 5 0.125 No Hit GCCTCGAGAAGAATTTGGGCACACTTCACCTGAAACAAATTGAAAGAAGC 5 0.125 No Hit GCTTGTTGCCACTCAAAAACGTAAACTGTCGTCGTATTGTAGTAGGAGGA 5 0.125 No Hit CCAAGACACAAGACTAAAGGAGGCACGCATAAGACATTAAAAAAACTAGT 5 0.125 No Hit ACTTGACGCGTGTTGTCGAATCCGATTATACGGATAAAGGCGTTAGGGTA 5 0.125 No Hit GCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTT 5 0.125 No Hit CCCAGTCGTCTTCCAGTAACCTTTCTCAGTAGCACGATTCGTCTTAGACC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.037500000000000006 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.7 0.0 0.0 0.0 0.0 90-91 0.7875000000000001 0.0 0.0 0.0 0.0 92-93 0.8374999999999999 0.0 0.0 0.0 0.0 94-95 0.925 0.0 0.0 0.0 0.0 96-97 1.0375 0.0 0.0 0.0 0.0 98-99 1.3875 0.0 0.0 0.0 0.0 100-101 1.675 0.0 0.0 0.0 0.0 102-103 1.875 0.0 0.0 0.0 0.0 104-105 2.0625 0.0 0.0 0.0 0.0 106-107 2.2375 0.0 0.0 0.0 0.0 108-109 2.4625 0.0 0.0 0.0 0.0 110-111 2.7875 0.0 0.0 0.0 0.0 112-113 3.125 0.0 0.0 0.0 0.0 114-115 3.4375 0.0 0.0 0.0 0.0 116-117 3.7125 0.0 0.0 0.0 0.0 118-119 4.1625 0.0 0.0 0.0 0.0 120-121 4.4625 0.0 0.0 0.0 0.0 122-123 4.85 0.0 0.0 0.0 0.0 124-125 5.525 0.0 0.0 0.0 0.0 126-127 5.949999999999999 0.0 0.0 0.0 0.0 128-129 6.387499999999999 0.0 0.0 0.0 0.0 130-131 6.775 0.0 0.0 0.0 0.0 132-133 7.3375 0.0 0.0 0.0 0.0 134-135 7.9125 0.0 0.0 0.0 0.0 136-137 8.462499999999999 0.0 0.0 0.0 0.0 138-139 9.3125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTTGCA 10 0.006830828 145.0 6 GTTGCAC 10 0.006830828 145.0 7 TTTTTAA 20 3.5877043E-4 108.75 5 >>END_MODULE SRR13695417 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695417_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.173 37.0 37.0 37.0 37.0 37.0 2 36.194 37.0 37.0 37.0 37.0 37.0 3 36.238 37.0 37.0 37.0 37.0 37.0 4 36.2785 37.0 37.0 37.0 37.0 37.0 5 36.259 37.0 37.0 37.0 37.0 37.0 6 36.284 37.0 37.0 37.0 37.0 37.0 7 36.366 37.0 37.0 37.0 37.0 37.0 8 36.351 37.0 37.0 37.0 37.0 37.0 9 36.365 37.0 37.0 37.0 37.0 37.0 10-14 36.3219 37.0 37.0 37.0 37.0 37.0 15-19 36.298 37.0 37.0 37.0 37.0 37.0 20-24 36.2495 37.0 37.0 37.0 37.0 37.0 25-29 36.2074 37.0 37.0 37.0 37.0 37.0 30-34 36.1438 37.0 37.0 37.0 37.0 37.0 35-39 36.155899999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.074 37.0 37.0 37.0 37.0 37.0 45-49 36.1637 37.0 37.0 37.0 37.0 37.0 50-54 36.0586 37.0 37.0 37.0 37.0 37.0 55-59 36.06589999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.0338 37.0 37.0 37.0 37.0 37.0 65-69 35.982000000000006 37.0 37.0 37.0 37.0 37.0 70-74 35.922599999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.978500000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.923500000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.8935 37.0 37.0 37.0 37.0 37.0 90-94 35.80500000000001 37.0 37.0 37.0 37.0 37.0 95-99 35.847899999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.783300000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.772 37.0 37.0 37.0 37.0 37.0 110-114 35.679899999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.777 37.0 37.0 37.0 37.0 37.0 120-124 35.655100000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.577600000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.433 37.0 37.0 37.0 37.0 37.0 135-139 35.47389999999999 37.0 37.0 37.0 37.0 37.0 140-144 35.3565 37.0 37.0 37.0 32.2 37.0 145-149 35.05585 37.0 37.0 37.0 27.4 37.0 150-151 34.868375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 8.0 24 4.0 25 14.0 26 7.0 27 10.0 28 12.0 29 23.0 30 27.0 31 42.0 32 57.0 33 100.0 34 206.0 35 606.0 36 2690.0 37 189.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.54942298043151 21.12393376818866 11.866532865027597 31.46011038635223 2 26.05 26.575 33.025 14.35 3 21.05 27.35 32.225 19.375 4 24.325 34.8 22.775000000000002 18.099999999999998 5 26.575 34.75 22.45 16.225 6 17.849999999999998 40.050000000000004 24.85 17.25 7 20.175 22.825 38.975 18.025 8 20.549999999999997 26.1 30.7 22.650000000000002 9 22.825 23.0 30.375000000000004 23.799999999999997 10-14 22.465 30.59 26.21 20.735 15-19 22.7 28.71 27.994999999999997 20.595 20-24 22.586293146573286 28.81940970485243 27.763881940970485 20.830415207603803 25-29 21.85592796398199 29.41470735367684 27.86893446723362 20.860430215107552 30-34 22.631315657828914 28.034017008504254 27.808904452226113 21.52576288144072 35-39 22.22111055527764 29.074537268634316 28.459229614807402 20.245122561280642 40-44 22.546273136568285 28.25912956478239 28.459229614807402 20.735367683841922 45-49 21.87093546773387 28.919459729864933 28.779389694847424 20.430215107553774 50-54 23.19159579789895 27.99399699849925 28.30915457728864 20.505252626313155 55-59 23.496748374187092 27.54377188594297 28.18409204602301 20.775387693846923 60-64 22.376188094047023 27.55377688844422 28.804402201100547 21.265632816408203 65-69 22.934173669467786 27.62104841936775 28.85654261704682 20.588235294117645 70-74 23.29664832416208 26.908454227113555 28.61930965482741 21.17558779389695 75-79 23.051525762881443 28.3791895947974 28.18409204602301 20.38519259629815 80-84 23.771885942971487 27.843921960980488 27.923961980990498 20.460230115057527 85-89 23.486743371685844 27.57878939469735 27.443721860930463 21.490745372686344 90-94 23.196598299149578 28.459229614807402 28.009004502251127 20.335167583791897 95-99 23.781890945472735 27.753876938469237 27.973986993496748 20.490245122561284 100-104 22.916458229114557 28.90945472736368 27.428714357178592 20.74537268634317 105-109 23.431715857928964 28.60430215107554 27.593796898449224 20.370185092546272 110-114 24.34217108554277 28.494247123561784 26.828414207103553 20.335167583791897 115-119 23.731865932966485 27.538769384692348 27.763881940970485 20.965482741370685 120-124 24.772386193096548 27.533766883441718 26.96348174087044 20.730365182591296 125-129 24.467233616808404 27.61880940470235 28.149074537268636 19.76488244122061 130-134 24.689751801441155 27.822257806244995 27.967373899119295 19.520616493194556 135-139 25.85551330798479 27.836702021212727 27.28637182309386 19.021412847708625 140-144 26.43321660830415 27.55377688844422 26.82341170585293 19.189594797398698 145-149 26.144379408674773 27.515133323327827 27.39506728700786 18.945419980989545 150-151 26.817669878613444 26.24202227505944 28.16919033913152 18.771117507195594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 0.5 11 0.0 12 0.0 13 1.0 14 1.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.5 21 3.5 22 5.0 23 2.5 24 1.5 25 4.0 26 6.0 27 11.0 28 13.0 29 12.5 30 13.5 31 22.0 32 36.5 33 42.5 34 61.0 35 75.0 36 103.0 37 131.0 38 144.5 39 172.0 40 211.0 41 239.0 42 257.0 43 284.0 44 285.5 45 278.0 46 239.0 47 208.0 48 210.0 49 193.0 50 165.5 51 124.5 52 89.5 53 69.0 54 62.5 55 47.0 56 32.5 57 30.0 58 20.5 59 22.0 60 20.5 61 15.5 62 12.0 63 6.5 64 3.5 65 1.5 66 1.0 67 0.5 68 0.0 69 0.5 70 0.5 71 1.0 72 1.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.05 30-34 0.05 35-39 0.05 40-44 0.05 45-49 0.05 50-54 0.05 55-59 0.05 60-64 0.05 65-69 0.04 70-74 0.05 75-79 0.05 80-84 0.05 85-89 0.05 90-94 0.05 95-99 0.05 100-104 0.05 105-109 0.05 110-114 0.05 115-119 0.05 120-124 0.05 125-129 0.05 130-134 0.08 135-139 0.06 140-144 0.05 145-149 0.055 150-151 0.11249999999999999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.07499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 69.58628401043609 46.675 2 19.194931047335075 25.75 3 7.007081625046589 14.099999999999998 4 2.459932910920611 6.6000000000000005 5 0.8199776369735371 2.75 6 0.5963473723443906 2.4 7 0.18635855385762207 0.8750000000000001 8 0.07454342154304883 0.4 9 0.07454342154304883 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AACAGACGTTTTTCTGTGCTTCATATTATCGAGAGAGAAAATGGCCGAAG 9 0.22499999999999998 No Hit GAAGCGTTTGAGGAATGAAGCAGTTGCAGAGCCAATTATTGGGGAATTGC 9 0.22499999999999998 No Hit GGTAAACCCTCCAACCAAACCAGCTACTTTAACCTTTCTTCAAGCTACTC 8 0.2 No Hit AGGAGATTGATGATCCTGAAGCTACAAAACCAGAAGATTGGGATGAAGAG 8 0.2 No Hit GCCCTAGAACAACCCAAGAAAAAGCAGCCTCCTTCAAAATCGATCTCTTT 7 0.17500000000000002 No Hit GGTTGAATTTGGTAAAGATTTTCTTTTGCAGCATCATTCAACCCGTAAAT 7 0.17500000000000002 No Hit GGGAGATGGAACATGTTTTGATGTTTTCCAGAAGTTTCTAGGTGTTCTTG 7 0.17500000000000002 No Hit ATAATCATCACTTCACTTTATTTCCTCTCTCTCTCAACAACCCCATTTCC 7 0.17500000000000002 No Hit GTTAAGTGTGCTGATGCACATGCAATTGCTGATGCTGCTTACAAAAGCGA 7 0.17500000000000002 No Hit CAAGCTGAGATTTGAGTACTCAGAATTTCCATTCAAGTGTGATGGCTGCA 6 0.15 No Hit CATGAGTGGAAAAGTCACTGAACACATCATTCCTTCTTGCAAAAAGATTG 6 0.15 No Hit GAATGACGTCCTTCACCAACGGCTCCAACAGTATGAGAACCGTTGGTCAG 6 0.15 No Hit AGACAGACATGTCCCTGCCAACTCTGCTAAACCCACAAACCCCAAAACTA 6 0.15 No Hit GTTATGACCTTCACCTCAAACCTGATCTTTCAGTCTGTACCTTTTCTGGC 6 0.15 No Hit GGCCGATATCAAAAGTGCTGGTTTTGTTGCACCAGGACCAGTGAAGGCAG 6 0.15 No Hit ATTGGATCTTCAATTGGGATCTCTACACCTGCAAAATCTGTTGCTAGATT 6 0.15 No Hit GTGGTATAATTAGAACCCTTGATGTCCCAATATATATCACTAAGATTTCT 6 0.15 No Hit CCGAGGACCATTTTTTAAGATCTTACTCAGCGCATTGTCAATTTCTGATT 6 0.15 No Hit GGAACAATGCCTGTTGGAGGGCCTGTTGATGCACTCCCTTACTGCTATAT 6 0.15 No Hit AAAACACCCAGAAGTGAAGTTATTTCAATCCTGAATTGCTTATGCAGTGA 6 0.15 No Hit AACTTTGTAAGATCAATGCAGTGGGAACAGAAGATGAGATTTTTGAAAAA 6 0.15 No Hit GGGAGTGTAATAACAAGTGGGTTTTCGTGGAATTTGTTTGCTATGACTGG 6 0.15 No Hit TTCTAAAAACTATTTTTCATTTCCTTTTCTCTCTAGAATATTAGATCTCC 6 0.15 No Hit CTCAAATTAGCTTCTGTACTTTTGATGGGACTCAGGGTGTCAGTATGAAC 6 0.15 No Hit CTTTCAAAGAAGGAGGCAACTGATAATATGCTTTCTGAAATAGGAGAGCA 6 0.15 No Hit CTCGCTTCTAGATGGTGAAGGACAGAATTTGCCTTATAGGAATTTGATGG 5 0.125 No Hit CAAACAACGAACAATGATCTTCATTTTTCTCTCACTTCTCCTTCTCGTTG 5 0.125 No Hit AATTTCTGCTCCAGATTTTGTTCACCTGGAGAAAACAAAGATTCAACTCC 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit GCTGTTTCCATCTCTTCTTCCAGTTCCCAGAAGACTGGGTTCCTCGTTGG 5 0.125 No Hit GGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCTTGACGGAGC 5 0.125 No Hit CTTGTATAAGAGATTATATCGATGTTACTGATCTTGTTGATGCTCATGTT 5 0.125 No Hit AAACAACAACAGAGAGCTGTCGGAAGAAAGAAATTAGAAGATGCACAATC 5 0.125 No Hit GAACCGTGAGCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTC 5 0.125 No Hit GGAGATGAAATCTCAGGAACTGGGACAGTTGCCTCGTCACTAACAAGTTA 5 0.125 No Hit ATTGAAGTTAGGGACATTAGCCTTGAAAACATTGAGTAAACCATTGGCCA 5 0.125 No Hit CTTGGCAAGGCCTTGAACGACATCATGGTCCAACAACACAACACTCTAAG 5 0.125 No Hit GCCCCTTCTAGTTGCAGAATCATTGGGCCGTCAGACATTATTGGAAATTT 5 0.125 No Hit GTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGG 5 0.125 No Hit CACAGGAACCTATTACGTCTGATAGGGTTTTGCACAACAACTACAGAACG 5 0.125 No Hit GCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCACACTTTCCTTCT 5 0.125 No Hit CTCATAATTGGTTGGTGCAACACTTGCTGCCTTGCAGGTTTAATGATCAG 5 0.125 No Hit TATTGGTCATCATAACCATGAGATCCGACTGGGATAAGGAGGCGGAAAAG 5 0.125 No Hit ATTGAAAGGAAATACTTGGTTCTGAGGAATATCAATTTAGCTGGAACAAT 5 0.125 No Hit GGTGGCTACATCATTTCAGATAACTCCTCTGGTAACAAGCCTGATGTCAT 5 0.125 No Hit CAAGAAAACAAAGCAAAAGAACCCTAACTTGCAAGTTGCAACATCTTCTT 5 0.125 No Hit GTCCTCCGTCTCCGAGGGGGTATGCAGATTTTTGTGAAGACCTTGACAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.5375 0.0 0.0 0.0 0.0 88-89 0.7250000000000001 0.0 0.0 0.0 0.0 90-91 0.8125 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 0.95 0.0 0.0 0.0 0.0 96-97 1.0625 0.0 0.0 0.0 0.0 98-99 1.4125 0.0 0.0 0.0 0.0 100-101 1.725 0.0 0.0 0.0 0.0 102-103 1.925 0.0 0.0 0.0 0.0 104-105 2.1125 0.0 0.0 0.0 0.0 106-107 2.2874999999999996 0.0 0.0 0.0 0.0 108-109 2.5125 0.0 0.0 0.0 0.0 110-111 2.8375 0.0 0.0 0.0 0.0 112-113 3.175 0.0 0.0 0.0 0.0 114-115 3.4875 0.0 0.0 0.0 0.0 116-117 3.7625 0.0 0.0 0.0 0.0 118-119 4.2125 0.0 0.0 0.0 0.0 120-121 4.512499999999999 0.0 0.0 0.0 0.0 122-123 4.875 0.0 0.0 0.0 0.0 124-125 5.55 0.0 0.0 0.0 0.0 126-127 6.0 0.0 0.0 0.0 0.0 128-129 6.4375 0.0 0.0 0.0 0.0 130-131 6.862500000000001 0.0 0.0 0.0 0.0 132-133 7.425 0.0 0.0 0.0 0.0 134-135 8.05 0.0 0.0 0.0 0.0 136-137 8.6375 0.0 0.0 0.0 0.0 138-139 9.5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTTAA 10 0.0068062083 145.15189 1 TAAATAC 10 0.007070461 143.3375 5 TTTTAAA 10 0.007070461 143.3375 2 TACTGCG 10 0.007070461 143.3375 9 TTGAAGA 10 0.007070461 143.3375 2 GGGGGGG 40 0.007605626 18.143986 140-144 AAAAAAA 70 3.8051072E-5 16.588789 80-84 >>END_MODULE Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189355 spots for SRR13695417.sra Written 1189355 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra Read 1189346 spots for SRR13695417.sra Written 1189346 spots for SRR13695417.sra SRR ids: ['SRR13695417.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_k35b74_5 SRR13695417.sra spots: 23786929 blocks: [[1, 1189346], [1189347, 2378692], [2378693, 3568038], [3568039, 4757384], [4757385, 5946730], [5946731, 7136076], [7136077, 8325422], [8325423, 9514768], [9514769, 10704114], [10704115, 11893460], [11893461, 13082806], [13082807, 14272152], [14272153, 15461498], [15461499, 16650844], [16650845, 17840190], [17840191, 19029536], [19029537, 20218882], [20218883, 21408228], [21408229, 22597574], [22597575, 23786929]] SRR13695417 file size 8062138 SRR13695417 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695417 SRR13695417_1.fastq SRR13695417_2.fastq Input file: SRR13695417_1.fastq Paired file: SRR13695417_2.fastq trimmed: SRR13695417-trimmed-pair1.fastq, SRR13695417-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:18:20 2025 >> started Wed Feb 12 01:18:47 2025 >> done (27.116s) 23786929 read pairs processed; of these: 170 ( 0.00%) short read pairs filtered out after trimming by size control 738 ( 0.00%) empty read pairs filtered out after trimming by size control 23786021 (100.00%) read pairs available; of these: 2920228 (12.28%) trimmed read pairs available after processing 20865793 (87.72%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 4 0.00% 20 3 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 2 0.00% 25 5 0.00% 26 3 0.00% 27 4 0.00% 28 1 0.00% 29 14 0.00% 30 11 0.00% 31 5 0.00% 32 5 0.00% 33 11 0.00% 34 18 0.00% 35 11 0.00% 36 23 0.00% 37 10 0.00% 38 20 0.00% 39 21 0.00% 40 22 0.00% 41 31 0.00% 42 26 0.00% 43 36 0.00% 44 36 0.00% 45 36 0.00% 46 55 0.00% 47 63 0.00% 48 57 0.00% 49 94 0.00% 50 89 0.00% 51 131 0.00% 52 152 0.00% 53 131 0.00% 54 165 0.00% 55 193 0.00% 56 188 0.00% 57 231 0.00% 58 277 0.00% 59 336 0.00% 60 389 0.00% 61 447 0.00% 62 524 0.00% 63 624 0.00% 64 729 0.00% 65 787 0.00% 66 819 0.00% 67 960 0.00% 68 1071 0.00% 69 1189 0.00% 70 1464 0.01% 71 1585 0.01% 72 1824 0.01% 73 2090 0.01% 74 2467 0.01% 75 2693 0.01% 76 2956 0.01% 77 3353 0.01% 78 3741 0.02% 79 4139 0.02% 80 4603 0.02% 81 5128 0.02% 82 6002 0.03% 83 6332 0.03% 84 7353 0.03% 85 8095 0.03% 86 8647 0.04% 87 9280 0.04% 88 10087 0.04% 89 10616 0.04% 90 11670 0.05% 91 12634 0.05% 92 13278 0.06% 93 14457 0.06% 94 15625 0.07% 95 16711 0.07% 96 17491 0.07% 97 18650 0.08% 98 19759 0.08% 99 20527 0.09% 100 21488 0.09% 101 22703 0.10% 102 23788 0.10% 103 24916 0.10% 104 26201 0.11% 105 27134 0.11% 106 28011 0.12% 107 30040 0.13% 108 31016 0.13% 109 32086 0.13% 110 33175 0.14% 111 34340 0.14% 112 35137 0.15% 113 36564 0.15% 114 38566 0.16% 115 40056 0.17% 116 41144 0.17% 117 42376 0.18% 118 44143 0.19% 119 44287 0.19% 120 46123 0.19% 121 47165 0.20% 122 48409 0.20% 123 49706 0.21% 124 50786 0.21% 125 52245 0.22% 126 54548 0.23% 127 55596 0.23% 128 55989 0.24% 129 57069 0.24% 130 58883 0.25% 131 59240 0.25% 132 60027 0.25% 133 61908 0.26% 134 62728 0.26% 135 63844 0.27% 136 65316 0.27% 137 67239 0.28% 138 67954 0.29% 139 70253 0.30% 140 70359 0.30% 141 71760 0.30% 142 72354 0.30% 143 72844 0.31% 144 75098 0.32% 145 75742 0.32% 146 77050 0.32% 147 77672 0.33% 148 79998 0.34% 149 80666 0.34% 150 81162 0.34% 151 20865793 87.72% 23786021 reads passed initial QC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.20 fanout-score-rank=24 prefix-density=0.35 prefix-fanout=2.1 sequence=TTAGCCTTTCTGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=521.60 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=17.6 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.15 fanout-score-rank=31 prefix-density=0.31 prefix-fanout=2.1 sequence=TGCAAGTGCGGCAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=121.86 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=9.7 sequence=AAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGGCCTCTCTGCTGACCCAGAGACCTTTGCCAAGAACCGTGAGCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTCTTGGATGCGTCTTCCCCGAGCTCTTGTCCCGCAACGGTGTCAAGTTCGGCGAGGCTGTATGGTTCAAGGCTGGAGCCCAGATCTTCAGCGAGGGTGGACTTGACTACTTGGGCAACCCAAGCTTGATCCACGCACAAAG SRR13695417 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:19:28 Started mapping on | Feb 12 01:19:28 Finished on | Feb 12 01:22:31 Mapping speed, Million of reads per hour | 467.92 Number of input reads | 23786021 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 22067039 Uniquely mapped reads % | 92.77% Average mapped length | 294.43 Number of splices: Total | 21858848 Number of splices: Annotated (sjdb) | 21368787 Number of splices: GT/AG | 21413593 Number of splices: GC/AG | 347651 Number of splices: AT/AC | 12662 Number of splices: Non-canonical | 84942 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 588030 % of reads mapped to multiple loci | 2.47% Number of reads mapped to too many loci | 177781 % of reads mapped to too many loci | 0.75% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.84% % of reads unmapped: other | 0.16% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1131250 1131250 1131250 N_multimapping 588030 588030 588030 N_noFeature 961705 21568861 1266908 N_ambiguous 330637 2214 136046 UnstrandedReadsAssigned:20774697 PositiveStrandReadsAssigned:495964 NegativeStrandReadsAssigned:20664085 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695417 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695417-trimmed-pair1.fastq SRR13695417-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,786,021 reads, 20,754,485 reads pseudoaligned [quant] estimated average fragment length: 245.875 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,163 rounds 52401 SRR13695417.ke.tsv 34699 SRR13695417.se.tsv 87100 total ==> SRR13695417.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1773.12 898 23.574 Potri.005G024800.1.v4.1 1035 790.125 424 24.9785 Potri.004G059700.1.v4.1 961 716.227 0 0 Potri.007G009000.2.v4.1 1416 1171.12 0 0 Potri.003G141000.2.v4.1 2943 2698.12 1088.88 18.7852 Potri.016G087400.1.v4.1 270 86.687 1055 566.493 Potri.015G069301.1.v4.1 564 327.586 0 0 Potri.010G195200.1.v4.1 1773 1528.12 119 3.6248 Potri.012G127500.1.v4.1 977 732.182 137 8.70959 ==> SRR13695417.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 179 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 283 Potri.001G212900.v4.1 17 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 20 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695417 completed mapping pipeline successfully