Starting /dee2/code/volunteer_pipeline.sh SRR13695418 current disk space = 3050504572928 free memory = 1433751464 SRR13695418 SRAfilesize e73e24f8122c1ca1d1535e21f648a36a SRR13695418.sra SRR13695418.sra file validated SRR13695418 is paired end SRR13695418 is conventional basespace SRR13695418 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695418_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.617 37.0 37.0 37.0 37.0 37.0 2 36.32025 37.0 37.0 37.0 37.0 37.0 3 36.4995 37.0 37.0 37.0 37.0 37.0 4 36.59 37.0 37.0 37.0 37.0 37.0 5 36.624 37.0 37.0 37.0 37.0 37.0 6 36.5875 37.0 37.0 37.0 37.0 37.0 7 36.5075 37.0 37.0 37.0 37.0 37.0 8 36.5465 37.0 37.0 37.0 37.0 37.0 9 36.564 37.0 37.0 37.0 37.0 37.0 10-14 36.5592 37.0 37.0 37.0 37.0 37.0 15-19 36.5043 37.0 37.0 37.0 37.0 37.0 20-24 36.498999999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.439499999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.4064 37.0 37.0 37.0 37.0 37.0 35-39 36.3673 37.0 37.0 37.0 37.0 37.0 40-44 36.3943 37.0 37.0 37.0 37.0 37.0 45-49 36.415800000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.361599999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.364999999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.3177 37.0 37.0 37.0 37.0 37.0 65-69 36.2734 37.0 37.0 37.0 37.0 37.0 70-74 36.290200000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.25150000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.181 37.0 37.0 37.0 37.0 37.0 85-89 36.2158 37.0 37.0 37.0 37.0 37.0 90-94 36.1257 37.0 37.0 37.0 37.0 37.0 95-99 36.1066 37.0 37.0 37.0 37.0 37.0 100-104 36.052 37.0 37.0 37.0 37.0 37.0 105-109 36.0354 37.0 37.0 37.0 37.0 37.0 110-114 36.026500000000006 37.0 37.0 37.0 37.0 37.0 115-119 35.9418 37.0 37.0 37.0 37.0 37.0 120-124 35.9731 37.0 37.0 37.0 37.0 37.0 125-129 35.9265 37.0 37.0 37.0 37.0 37.0 130-134 35.932100000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.8568 37.0 37.0 37.0 37.0 37.0 140-144 35.7904 37.0 37.0 37.0 37.0 37.0 145-149 35.597500000000004 37.0 37.0 37.0 37.0 37.0 150-151 35.39 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 3.0 24 4.0 25 1.0 26 6.0 27 9.0 28 18.0 29 31.0 30 24.0 31 27.0 32 50.0 33 72.0 34 126.0 35 334.0 36 2934.0 37 361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.75 11.65 8.125 49.475 2 17.935192162773173 15.699572971615172 38.30695805074102 28.058276814870638 3 18.4 16.150000000000002 25.8 39.65 4 21.05 26.125 23.325000000000003 29.5 5 24.2 31.775 24.2 19.825 6 18.525 33.6 25.674999999999997 22.2 7 15.425 28.575 38.9 17.1 8 17.025000000000002 27.325 31.900000000000002 23.75 9 17.45 21.875 37.15 23.525 10-14 19.115 30.270000000000003 27.865000000000002 22.75 15-19 19.6 28.64 27.834999999999997 23.925 20-24 19.59 28.549999999999997 27.67 24.19 25-29 19.57 28.77 27.96 23.7 30-34 19.285 28.389999999999997 28.325 24.0 35-39 20.31 28.84 27.485 23.365 40-44 19.515 28.76 27.925 23.799999999999997 45-49 20.02 28.64 27.644999999999996 23.695 50-54 19.73 28.185 28.115000000000002 23.97 55-59 20.135 28.73 27.625 23.51 60-64 20.369999999999997 29.099999999999998 27.325 23.205000000000002 65-69 19.81 28.694999999999997 27.905 23.59 70-74 20.335 28.435 28.015 23.215 75-79 20.27 27.88 28.235 23.615 80-84 20.26 29.465000000000003 27.575 22.7 85-89 20.595 29.18 27.529999999999998 22.695 90-94 20.005 28.78 27.944999999999997 23.27 95-99 19.814999999999998 28.895 27.134999999999998 24.154999999999998 100-104 20.665 28.725 27.465 23.145 105-109 20.369999999999997 28.560000000000002 27.83 23.24 110-114 21.025 28.515 27.33 23.13 115-119 21.7 27.915 27.72 22.665 120-124 20.34 28.65 27.625 23.385 125-129 20.915 29.099999999999998 27.13 22.855 130-134 21.07 29.675 26.58 22.675 135-139 21.475 28.939999999999998 26.840000000000003 22.745 140-144 21.044999999999998 28.585 26.995 23.375 145-149 21.21 28.4 26.640000000000004 23.75 150-151 21.775 27.462500000000002 26.950000000000003 23.8125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.5 6 0.5 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 2.0 17 1.5 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 3.5 25 6.5 26 4.5 27 6.5 28 12.0 29 18.0 30 20.5 31 28.5 32 32.0 33 38.5 34 61.5 35 86.0 36 112.0 37 127.0 38 138.0 39 161.5 40 196.5 41 217.5 42 235.0 43 265.5 44 262.5 45 247.0 46 243.0 47 223.0 48 207.0 49 197.0 50 172.5 51 153.0 52 130.5 53 92.5 54 74.0 55 51.5 56 35.0 57 31.0 58 23.5 59 27.0 60 21.0 61 12.0 62 7.5 63 3.0 64 2.5 65 1.5 66 1.0 67 1.0 68 0.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.8 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98050139275766 52.400000000000006 2 18.871866295264624 27.1 3 5.43175487465181 11.700000000000001 4 1.7757660167130918 5.1 5 0.5571030640668524 2.0 6 0.31337047353760444 1.35 7 0.06963788300835655 0.35000000000000003 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCAATCGGCACTCCATCTTGTAAGACTCAGTTTAGTTAACTTATCGGC 7 0.17500000000000002 No Hit CATCCTTTGCCTCTCCACTCCCAATAACTCTCTCCACGTTTACTATTTTG 7 0.17500000000000002 No Hit CAGGTAGACAGGAGCGCCGGCGCCAACACGCTCAGCATACTTGCCGGCCT 6 0.15 No Hit CCAGTGACAACATAAATGTATCTCCCATCTGTGACCATTCCCAAGTGTGA 6 0.15 No Hit CTCCATTATCACATGGCAAGGTGGAAACCCCAGCTGCAGAAACCCCAGCT 6 0.15 No Hit GTCACCACACCACTCTTCCCAATTGCAGAAGTCCGGTACCCTTCAAGATT 6 0.15 No Hit TTTTTTTTTAGTCGGTCTAAATAGTGAGTATCATGATCAAATAAGAATGG 6 0.15 No Hit TCCATGGCGAGGAACTTGACTGGCTTCTTCATTGGCATCACAGGGATCTT 6 0.15 No Hit GCAATTACTTCCACTTATGCAAGAGCCCAGACCCTGAAAACAACTTAAAA 6 0.15 No Hit CACTGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTGTTTCCCT 6 0.15 No Hit TTATGATTTAAAATCGTTACCAAAAAAGAGAGTAAAACAAGACAATATAA 6 0.15 No Hit CTGTAACCTTAAGACTCGGGTCCAACTTTCCTGCTAGAGCCAACAAAAGA 5 0.125 No Hit CAACAACAAAATCAACGGTGATGATGATGAGAAAGAGAAGGGAAATTTGG 5 0.125 No Hit CCCAGCTGCTTCAGCTACCTCTGGAATCACCATGGAAGCTGTAAGTGCAC 5 0.125 No Hit GTATGCAGCTGTCATCTCCAAATGCAACCCCTGGGACTAATGCAACCTGG 5 0.125 No Hit GGTGGCGACAACAGCAGCACCGACTTCTTTCAATGAAGCCTTGATGCTGA 5 0.125 No Hit GCCATGTCCCGAGCATTGTCATAATCATTCAGCTCAACGAGCATCTTCTT 5 0.125 No Hit CCAACTAATAAACACCCTTTAGGAATTTTAGCTCCTAAGGCTGTGTACTT 5 0.125 No Hit TTTCCCTTTCCTCGTCTGTCATCTCGTCCTTAAAAAACTCTTCACCAAAC 5 0.125 No Hit CCCAGAGCTCAAAACTGCCTTTGACAAAATCATAATAACCACCCTTCAGT 5 0.125 No Hit CAAGAACAACAACAGGTACAAGCAACATAGAATTCTACTAAATAGAGTTA 5 0.125 No Hit GGCTGATTCCAGTCCTTGCCCTGATAATGTGTGGGCGAAATCCCCTGAAA 5 0.125 No Hit GCCAGAACTATAAATAAGGTTTTGGAGTTCCTCGAAGCAGCAAAGATGAT 5 0.125 No Hit GTGGGATGTTGGAAATTCTAAGTGTTTCATTGACGACCATTTGGGTAAAA 5 0.125 No Hit TCCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGT 5 0.125 No Hit TTTCACAGCCAACAGGAAAGACGATCTGTTCTCAATTTCCCCTATCATGT 5 0.125 No Hit GTCCATACTTTTTAAGCACACAACCTTGCTTGCTTCTTCTAATCCACTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.5375000000000001 0.0 0.0 0.0 0.0 94-95 0.6625 0.0 0.0 0.0 0.0 96-97 0.75 0.0 0.0 0.0 0.0 98-99 1.05 0.0 0.0 0.0 0.0 100-101 1.2125 0.0 0.0 0.0 0.0 102-103 1.4125 0.0 0.0 0.0 0.0 104-105 1.7125 0.0 0.0 0.0 0.0 106-107 2.05 0.0 0.0 0.0 0.0 108-109 2.4375 0.0 0.0 0.0 0.0 110-111 2.8875 0.0 0.0 0.0 0.0 112-113 3.1125 0.0 0.0 0.0 0.0 114-115 3.575 0.0 0.0 0.0 0.0 116-117 3.7875 0.0 0.0 0.0 0.0 118-119 4.15 0.0 0.0 0.0 0.0 120-121 4.525 0.0 0.0 0.0 0.0 122-123 4.7375 0.0 0.0 0.0 0.0 124-125 5.125 0.0 0.0 0.0 0.0 126-127 5.725 0.0 0.0 0.0 0.0 128-129 6.3125 0.0 0.0 0.0 0.0 130-131 6.775 0.0 0.0 0.0 0.0 132-133 7.2875 0.0 0.0 0.0 0.0 134-135 7.800000000000001 0.0 0.0 0.0 0.0 136-137 8.2375 0.0 0.0 0.0 0.0 138-139 8.8625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTTG 10 0.006830828 145.0 6 GTTTGGG 10 0.006830828 145.0 8 TGTTTGG 10 0.006830828 145.0 7 TCGTGTT 10 0.006830828 145.0 4 TTCCTGT 10 0.006830828 145.0 145 GCTGTCA 10 0.006830828 145.0 8 GCTCGTG 10 0.006830828 145.0 2 TGCTCGT 10 0.006830828 145.0 1 CCACATA 10 0.006830828 145.0 9 TTCCACA 10 0.006830828 145.0 7 GGTAGTT 10 0.006830828 145.0 1 CGTGTTT 10 0.006830828 145.0 5 AAAGATA 10 0.006830828 145.0 145 CTCGTGT 10 0.006830828 145.0 3 >>END_MODULE SRR13695418 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695418_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1425 37.0 37.0 37.0 37.0 37.0 2 36.2655 37.0 37.0 37.0 37.0 37.0 3 36.09 37.0 37.0 37.0 37.0 37.0 4 36.1715 37.0 37.0 37.0 37.0 37.0 5 36.364 37.0 37.0 37.0 37.0 37.0 6 36.2505 37.0 37.0 37.0 37.0 37.0 7 36.3565 37.0 37.0 37.0 37.0 37.0 8 36.3855 37.0 37.0 37.0 37.0 37.0 9 36.256 37.0 37.0 37.0 37.0 37.0 10-14 36.294399999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.211400000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.201 37.0 37.0 37.0 37.0 37.0 25-29 36.1776 37.0 37.0 37.0 37.0 37.0 30-34 36.161899999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.148199999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.13365 37.0 37.0 37.0 37.0 37.0 45-49 36.096500000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.0364 37.0 37.0 37.0 37.0 37.0 55-59 36.043899999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.0162 37.0 37.0 37.0 37.0 37.0 65-69 35.9718 37.0 37.0 37.0 37.0 37.0 70-74 35.895500000000006 37.0 37.0 37.0 37.0 37.0 75-79 35.8831 37.0 37.0 37.0 37.0 37.0 80-84 35.9759 37.0 37.0 37.0 37.0 37.0 85-89 35.838800000000006 37.0 37.0 37.0 37.0 37.0 90-94 35.8425 37.0 37.0 37.0 37.0 37.0 95-99 35.839600000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.7888 37.0 37.0 37.0 37.0 37.0 105-109 35.795100000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.6906 37.0 37.0 37.0 37.0 37.0 115-119 35.6919 37.0 37.0 37.0 37.0 37.0 120-124 35.5632 37.0 37.0 37.0 37.0 37.0 125-129 35.57965 37.0 37.0 37.0 37.0 37.0 130-134 35.478899999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.40815 37.0 37.0 37.0 34.6 37.0 140-144 35.3835 37.0 37.0 37.0 34.6 37.0 145-149 35.171299999999995 37.0 37.0 37.0 27.4 37.0 150-151 34.991625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 2.0 17 1.0 18 2.0 19 2.0 20 3.0 21 3.0 22 3.0 23 7.0 24 3.0 25 6.0 26 6.0 27 9.0 28 6.0 29 19.0 30 33.0 31 45.0 32 52.0 33 111.0 34 208.0 35 640.0 36 2644.0 37 194.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.734939759036145 20.230923694779115 14.65863453815261 31.37550200803213 2 26.924999999999997 25.35 32.4 15.325 3 20.875 26.75 32.375 20.0 4 22.900000000000002 33.25 24.425 19.425 5 25.6 35.975 22.575 15.85 6 19.7 38.775 23.75 17.775 7 18.125 22.55 40.699999999999996 18.625 8 21.275 24.0 30.625000000000004 24.099999999999998 9 21.175 23.025000000000002 32.25 23.549999999999997 10-14 22.115000000000002 29.854999999999997 27.32 20.71 15-19 23.345 28.285 27.405 20.965 20-24 21.992199219921993 28.497849784978495 28.44784478447845 21.062106210621064 25-29 21.95097548774387 28.7743871935968 27.99899949974988 21.275637818909455 30-34 22.8245649129826 28.325665133026607 28.210642128425683 20.63912782556511 35-39 22.36223622362236 28.70787078707871 27.84278427842784 21.087108710871085 40-44 22.465616404101024 28.367091772943237 28.00700175043761 21.16029007251813 45-49 22.879575915183036 28.52070414082817 27.4004800960192 21.199239847969594 50-54 23.235 28.215 27.57 20.979999999999997 55-59 22.851425712856425 28.569284642321165 27.953976988494244 20.625312656328166 60-64 22.847284728472847 28.03780378037804 27.742774277427745 21.372137213721373 65-69 22.775000000000002 27.889999999999997 28.689999999999998 20.645 70-74 22.641320660330166 28.194097048524263 27.888944472236116 21.275637818909455 75-79 22.84185255576673 27.718315494648394 28.55856757027108 20.881264379313794 80-84 22.939999999999998 28.599999999999998 27.76 20.7 85-89 23.45438175270108 28.371348539415763 27.36594637855142 20.80832332933173 90-94 23.347334733473346 27.35273527352735 28.292829282928295 21.007100710071008 95-99 22.835 27.529999999999998 28.449999999999996 21.185000000000002 100-104 23.091545772886445 28.059029514757377 27.86893446723362 20.98049024512256 105-109 23.85954381752701 27.395958383353342 28.12625050020008 20.618247298919567 110-114 23.097309730973098 28.72787278727873 27.84278427842784 20.332033203320332 115-119 23.241972591777532 28.84365309592878 27.618285485645693 20.296088826647996 120-124 23.96698349174587 28.91445722861431 27.138569284642323 19.9799899949975 125-129 24.253488721052367 28.90011504026409 27.134497073975893 19.711899164707646 130-134 24.56473884330598 28.38202921753052 27.746647988793278 19.30658395037022 135-139 25.55905748161489 28.550702886587626 26.824753614487967 19.06548601730952 140-144 25.70757075707571 27.42774277427743 27.787778777877786 19.076907690769076 145-149 26.468234117058532 26.993496748374184 26.638319159579787 19.899949974987493 150-151 26.46654158849281 28.067542213883677 26.666666666666668 18.79924953095685 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 2.5 18 2.5 19 0.5 20 0.5 21 1.5 22 4.0 23 6.0 24 4.5 25 3.5 26 6.5 27 9.5 28 9.0 29 11.0 30 16.5 31 24.0 32 37.0 33 44.5 34 66.0 35 89.5 36 92.0 37 101.0 38 139.0 39 175.0 40 196.0 41 212.5 42 233.5 43 260.0 44 277.5 45 285.5 46 265.5 47 249.5 48 226.5 49 182.5 50 159.5 51 141.5 52 117.5 53 95.0 54 69.0 55 42.0 56 31.5 57 25.0 58 19.5 59 21.0 60 12.5 61 4.0 62 5.0 63 4.0 64 1.5 65 4.5 66 3.0 67 2.0 68 2.0 69 0.5 70 1.0 71 0.5 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.05 30-34 0.02 35-39 0.01 40-44 0.025 45-49 0.02 50-54 0.0 55-59 0.05 60-64 0.01 65-69 0.0 70-74 0.05 75-79 0.03 80-84 0.0 85-89 0.04 90-94 0.01 95-99 0.0 100-104 0.05 105-109 0.04 110-114 0.01 115-119 0.03 120-124 0.05 125-129 0.034999999999999996 130-134 0.06 135-139 0.055 140-144 0.01 145-149 0.05 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.89999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 73.12239221140473 52.575 2 18.67176634214186 26.85 3 5.632823365785813 12.15 4 1.6689847009735743 4.8 5 0.48678720445062584 1.7500000000000002 6 0.34770514603616137 1.5 7 0.03477051460361613 0.17500000000000002 8 0.03477051460361613 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTGTGTGCTCGTACATGTACATCAGGAAAAAAGAGAAGGAAAAAA 8 0.2 No Hit CTCACAACTCCACCGCCACGTTAGTCCTCCTCTATGTTAAGCCCCCGCCA 7 0.17500000000000002 No Hit CTGATTTCATAGAAGACTGGGTCAAAGTTTGCTACCCTGCCAAGACTAAG 6 0.15 No Hit CCAGGATGAAGCTTGGGTGAAACTAAGTGGAGGTCCGAACCGACTGATGT 6 0.15 No Hit CTTAGAGGAAGTCGAGCAACATCCCTCGTTTCTAACAACTCTCCACTACC 6 0.15 No Hit AGGAGATTGTGAAAAAAGAAAGGCAGAAGCAAGTTCAGTAATGGCAGCCT 6 0.15 No Hit CTCTCAAGCCGCTGTAAGTGTCGTCACATTCATTTAAGTTGCCTGTTTTA 6 0.15 No Hit CCCACCATCAACTTGTATACATATCGATAGAGTGGTCGCTGGGCAGACAA 6 0.15 No Hit GTTTCTTCTACAGAAGAAGATGAATGGGAGACAGTGGGGCCAAAAAACAA 6 0.15 No Hit TGGGGCTGCAATACAAATTAAGGATCTTCTATATGTTTTTGCTGGATATG 6 0.15 No Hit CAATGGTTAAAGCAACTAATGCCACTGAGTTTAGGGCAATCGTTCAAGAA 6 0.15 No Hit CCTCTCCCAAAAGAAATACTCTCTCCACATTTCGGTTCGACTATTTGTTA 6 0.15 No Hit GTTCCATTCAATTGCGGTAGGGGTCAACGATATTGCGTACCTAGCCAACC 5 0.125 No Hit GTGGTGATAAAGTTCACCGGGCTCCGGAACTAGTAGAATTTTATCAGAGT 5 0.125 No Hit CAAGTTGCAGCCACTGGTGGATTTCATTTTATCAATAGGAAGGACACTGG 5 0.125 No Hit TTTAGATTTCAGCCATAACTTTCGTTCTGTTTATAAATAAGAGAAGATGA 5 0.125 No Hit AGAGAGGGAATTGAGACTCAAAGATTTCAGCTGGGTAACTAATGGACTCA 5 0.125 No Hit GTCAGGTTTGATCATTTAACAATTGAAGCTGACTGCCACTTTGGCACCAG 5 0.125 No Hit GAAGTTTCAAGAAGTGCCGGAAACCGGTGTGACGTTTGCTGATGTGGCCG 5 0.125 No Hit TCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTG 5 0.125 No Hit GCTTCTTGTTGAGAAGAGAGTAGAGAGGGCAGGAGCGAAGATCAGAGAAA 5 0.125 No Hit GGATGGGACTACATCCGGGATGGACCAGCATTGTACAAAAACATGGACCG 5 0.125 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 5 0.125 No Hit CACAAACAAGACCAGCAAAAACCAGGACAAAAAAAGTTCAAGAATGGCCA 5 0.125 No Hit AAGCAGTGTCCACCATGGTGACAGCATTCAGGGAGCGGAGAGATTTCTTG 5 0.125 No Hit GTTGACACAGAAATCTTCTTGAGAGGAAAAAAGCAGATCGAGACTTTCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.42500000000000004 0.0 0.0 0.0 0.0 92-93 0.4875 0.0 0.0 0.0 0.0 94-95 0.6125 0.0 0.0 0.0 0.0 96-97 0.7 0.0 0.0 0.0 0.0 98-99 1.0 0.0 0.0 0.0 0.0 100-101 1.1625 0.0 0.0 0.0 0.0 102-103 1.3624999999999998 0.0 0.0 0.0 0.0 104-105 1.6625 0.0 0.0 0.0 0.0 106-107 2.025 0.0 0.0 0.0 0.0 108-109 2.4124999999999996 0.0 0.0 0.0 0.0 110-111 2.8625 0.0 0.0 0.0 0.0 112-113 3.0875 0.0 0.0 0.0 0.0 114-115 3.55 0.0 0.0 0.0 0.0 116-117 3.7625 0.0 0.0 0.0 0.0 118-119 4.15 0.0 0.0 0.0 0.0 120-121 4.5125 0.0 0.0 0.0 0.0 122-123 4.7125 0.0 0.0 0.0 0.0 124-125 5.125 0.0 0.0 0.0 0.0 126-127 5.75 0.0 0.0 0.0 0.0 128-129 6.3125 0.0 0.0 0.0 0.0 130-131 6.775 0.0 0.0 0.0 0.0 132-133 7.3625 0.0 0.0 0.0 0.0 134-135 7.8625 0.0 0.0 0.0 0.0 136-137 8.2875 0.0 0.0 0.0 0.0 138-139 8.925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACCA 10 0.006830828 145.0 3 CACACCG 10 0.006830828 145.0 8 TGTCCAC 10 0.006830828 145.0 7 GGTCACC 10 0.006830828 145.0 2 AGCAGTG 10 0.006830828 145.0 2 AACCACA 10 0.006830828 145.0 5 AGTGTCC 10 0.006830828 145.0 5 GAACCAC 10 0.006830828 145.0 4 CCACACC 10 0.006830828 145.0 7 GTCCACC 10 0.006830828 145.0 8 TCCACCA 10 0.006830828 145.0 9 TCACCAG 10 0.006830828 145.0 4 CGGTCAC 10 0.006830828 145.0 1 CAGTGTC 10 0.006830828 145.0 4 AAGCAGT 10 0.006830828 145.0 1 GGGGGGG 40 0.0076550315 18.125 140-144 >>END_MODULE Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846300 spots for SRR13695418.sra Written 846300 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra Read 846284 spots for SRR13695418.sra Written 846284 spots for SRR13695418.sra SRR ids: ['SRR13695418.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_e58kdteb SRR13695418.sra spots: 16925696 blocks: [[1, 846284], [846285, 1692568], [1692569, 2538852], [2538853, 3385136], [3385137, 4231420], [4231421, 5077704], [5077705, 5923988], [5923989, 6770272], [6770273, 7616556], [7616557, 8462840], [8462841, 9309124], [9309125, 10155408], [10155409, 11001692], [11001693, 11847976], [11847977, 12694260], [12694261, 13540544], [13540545, 14386828], [14386829, 15233112], [15233113, 16079396], [16079397, 16925696]] SRR13695418 file size 5730391 SRR13695418 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695418 SRR13695418_1.fastq SRR13695418_2.fastq Input file: SRR13695418_1.fastq Paired file: SRR13695418_2.fastq trimmed: SRR13695418-trimmed-pair1.fastq, SRR13695418-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:08:47 2025 >> started Wed Feb 12 02:09:07 2025 >> done (20.249s) 16925696 read pairs processed; of these: 123 ( 0.00%) short read pairs filtered out after trimming by size control 801 ( 0.00%) empty read pairs filtered out after trimming by size control 16924772 (99.99%) read pairs available; of these: 1922319 (11.36%) trimmed read pairs available after processing 15002453 (88.64%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 2 0.00% 20 0 0.00% 21 1 0.00% 22 2 0.00% 23 2 0.00% 24 2 0.00% 25 3 0.00% 26 1 0.00% 27 2 0.00% 28 4 0.00% 29 1 0.00% 30 3 0.00% 31 6 0.00% 32 3 0.00% 33 3 0.00% 34 3 0.00% 35 3 0.00% 36 4 0.00% 37 7 0.00% 38 6 0.00% 39 12 0.00% 40 26 0.00% 41 9 0.00% 42 10 0.00% 43 20 0.00% 44 14 0.00% 45 26 0.00% 46 30 0.00% 47 41 0.00% 48 32 0.00% 49 54 0.00% 50 57 0.00% 51 69 0.00% 52 78 0.00% 53 96 0.00% 54 88 0.00% 55 89 0.00% 56 129 0.00% 57 127 0.00% 58 172 0.00% 59 233 0.00% 60 216 0.00% 61 285 0.00% 62 329 0.00% 63 357 0.00% 64 404 0.00% 65 482 0.00% 66 534 0.00% 67 582 0.00% 68 721 0.00% 69 752 0.00% 70 889 0.01% 71 1049 0.01% 72 1158 0.01% 73 1394 0.01% 74 1521 0.01% 75 1728 0.01% 76 1880 0.01% 77 2153 0.01% 78 2334 0.01% 79 2575 0.02% 80 2908 0.02% 81 3339 0.02% 82 3799 0.02% 83 4231 0.02% 84 4766 0.03% 85 5274 0.03% 86 5477 0.03% 87 6041 0.04% 88 6433 0.04% 89 6913 0.04% 90 7459 0.04% 91 8136 0.05% 92 8665 0.05% 93 9119 0.05% 94 10126 0.06% 95 10749 0.06% 96 11469 0.07% 97 12209 0.07% 98 12742 0.08% 99 13237 0.08% 100 14162 0.08% 101 14462 0.09% 102 15518 0.09% 103 16060 0.09% 104 16671 0.10% 105 17461 0.10% 106 18736 0.11% 107 19430 0.11% 108 20300 0.12% 109 21065 0.12% 110 21559 0.13% 111 22139 0.13% 112 23537 0.14% 113 23669 0.14% 114 24526 0.14% 115 26235 0.16% 116 27069 0.16% 117 27739 0.16% 118 28783 0.17% 119 29320 0.17% 120 30236 0.18% 121 30795 0.18% 122 31802 0.19% 123 32626 0.19% 124 33172 0.20% 125 34450 0.20% 126 35930 0.21% 127 35920 0.21% 128 37128 0.22% 129 37992 0.22% 130 38925 0.23% 131 39380 0.23% 132 39478 0.23% 133 40681 0.24% 134 41582 0.25% 135 42688 0.25% 136 43077 0.25% 137 44080 0.26% 138 45211 0.27% 139 46861 0.28% 140 47457 0.28% 141 47394 0.28% 142 48260 0.29% 143 48277 0.29% 144 49244 0.29% 145 50303 0.30% 146 50714 0.30% 147 52382 0.31% 148 53187 0.31% 149 53861 0.32% 150 54877 0.32% 151 15002453 88.64% 16924772 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=21 prefix-density=0.24 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=545.79 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=18.7 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=26 prefix-density=0.54 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.11 sequence-density-rank=23 fanout-score=40.76 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=13.2 sequence=AAAGAAAAGAAAA SRR13695418 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:09:46 Started mapping on | Feb 12 02:09:47 Finished on | Feb 12 02:12:04 Mapping speed, Million of reads per hour | 444.74 Number of input reads | 16924772 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 15661687 Uniquely mapped reads % | 92.54% Average mapped length | 294.88 Number of splices: Total | 15541408 Number of splices: Annotated (sjdb) | 15161523 Number of splices: GT/AG | 15230274 Number of splices: GC/AG | 239447 Number of splices: AT/AC | 9904 Number of splices: Non-canonical | 61783 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.86 Insertion rate per base | 0.02% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 439142 % of reads mapped to multiple loci | 2.59% Number of reads mapped to too many loci | 114302 % of reads mapped to too many loci | 0.68% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.05% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 824158 824158 824158 N_multimapping 439142 439142 439142 N_noFeature 648558 15260044 913738 N_ambiguous 257123 2007 119251 UnstrandedReadsAssigned:14756006 PositiveStrandReadsAssigned:399636 NegativeStrandReadsAssigned:14628698 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695418 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695418-trimmed-pair1.fastq SRR13695418-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,924,772 reads, 14,672,639 reads pseudoaligned [quant] estimated average fragment length: 253.341 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,206 rounds 52401 SRR13695418.ke.tsv 34699 SRR13695418.se.tsv 87100 total ==> SRR13695418.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1765.66 694 25.1947 Potri.005G024800.1.v4.1 1035 782.659 408 33.4152 Potri.004G059700.1.v4.1 961 708.801 1 0.090434 Potri.007G009000.2.v4.1 1416 1163.66 0 0 Potri.003G141000.2.v4.1 2943 2690.66 650.399 15.4945 Potri.016G087400.1.v4.1 270 84.4323 1360 1032.49 Potri.015G069301.1.v4.1 564 322.247 0 0 Potri.010G195200.1.v4.1 1773 1520.66 365 15.3857 Potri.012G127500.1.v4.1 977 724.725 157 13.8862 ==> SRR13695418.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 151 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 194 Potri.001G212900.v4.1 5 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 17 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 8 SRR13695418 completed mapping pipeline successfully