Starting /dee2/code/volunteer_pipeline.sh SRR13695419 current disk space = 3050872803328 free memory = 1493872120 SRR13695419 SRAfilesize 2ff0abf1255c6f1842fbababb92c8020 SRR13695419.sra SRR13695419.sra file validated SRR13695419 is paired end SRR13695419 is conventional basespace SRR13695419 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695419_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.651 37.0 37.0 37.0 37.0 37.0 2 36.408 37.0 37.0 37.0 37.0 37.0 3 36.5675 37.0 37.0 37.0 37.0 37.0 4 36.5475 37.0 37.0 37.0 37.0 37.0 5 36.539 37.0 37.0 37.0 37.0 37.0 6 36.671 37.0 37.0 37.0 37.0 37.0 7 36.601 37.0 37.0 37.0 37.0 37.0 8 36.57 37.0 37.0 37.0 37.0 37.0 9 36.5305 37.0 37.0 37.0 37.0 37.0 10-14 36.5569 37.0 37.0 37.0 37.0 37.0 15-19 36.52760000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.511 37.0 37.0 37.0 37.0 37.0 25-29 36.4819 37.0 37.0 37.0 37.0 37.0 30-34 36.4055 37.0 37.0 37.0 37.0 37.0 35-39 36.4 37.0 37.0 37.0 37.0 37.0 40-44 36.3356 37.0 37.0 37.0 37.0 37.0 45-49 36.3323 37.0 37.0 37.0 37.0 37.0 50-54 36.3078 37.0 37.0 37.0 37.0 37.0 55-59 36.376999999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.3553 37.0 37.0 37.0 37.0 37.0 65-69 36.280699999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.2673 37.0 37.0 37.0 37.0 37.0 75-79 36.2841 37.0 37.0 37.0 37.0 37.0 80-84 36.1813 37.0 37.0 37.0 37.0 37.0 85-89 36.181 37.0 37.0 37.0 37.0 37.0 90-94 36.169799999999995 37.0 37.0 37.0 37.0 37.0 95-99 36.132600000000004 37.0 37.0 37.0 37.0 37.0 100-104 36.138999999999996 37.0 37.0 37.0 37.0 37.0 105-109 36.104400000000005 37.0 37.0 37.0 37.0 37.0 110-114 36.086299999999994 37.0 37.0 37.0 37.0 37.0 115-119 36.0944 37.0 37.0 37.0 37.0 37.0 120-124 35.9968 37.0 37.0 37.0 37.0 37.0 125-129 35.9583 37.0 37.0 37.0 37.0 37.0 130-134 36.00580000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.8938 37.0 37.0 37.0 37.0 37.0 140-144 35.8266 37.0 37.0 37.0 37.0 37.0 145-149 35.6409 37.0 37.0 37.0 37.0 37.0 150-151 35.398250000000004 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 2.0 23 5.0 24 6.0 25 6.0 26 5.0 27 11.0 28 13.0 29 21.0 30 22.0 31 34.0 32 42.0 33 69.0 34 109.0 35 304.0 36 2958.0 37 392.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.325 13.875000000000002 9.2 39.6 2 22.617853560682047 13.866599799398195 32.873620862587764 30.641925777331995 3 18.9 17.4 24.8 38.9 4 23.0 23.474999999999998 22.25 31.275 5 23.275000000000002 28.449999999999996 26.5 21.775 6 24.25 31.474999999999998 24.349999999999998 19.925 7 17.175 28.249999999999996 38.574999999999996 16.0 8 18.224999999999998 27.85 31.7 22.225 9 18.075 26.474999999999998 34.325 21.125 10-14 19.59 30.805 27.250000000000004 22.355 15-19 20.46 27.655 28.57 23.315 20-24 19.37 28.565 28.845 23.22 25-29 20.05 28.685 27.229999999999997 24.035 30-34 19.25 29.585 27.169999999999998 23.995 35-39 19.794999999999998 29.07 27.785 23.35 40-44 20.585 28.78 26.974999999999998 23.66 45-49 20.48 28.82 27.04 23.66 50-54 20.835 28.415000000000003 27.68 23.07 55-59 21.51 28.48 26.545 23.465 60-64 20.349999999999998 27.994999999999997 27.560000000000002 24.095 65-69 20.375 29.28 27.05 23.294999999999998 70-74 21.705 28.275 26.595000000000002 23.425 75-79 20.474999999999998 27.54 27.515 24.47 80-84 21.065 27.1 27.625 24.21 85-89 21.39 27.505000000000003 27.49 23.615 90-94 21.92 27.284999999999997 27.07 23.724999999999998 95-99 20.895 28.23 27.0 23.875 100-104 21.165 28.244999999999997 26.33 24.26 105-109 21.08 28.435 27.465 23.02 110-114 21.42 27.35 27.134999999999998 24.095 115-119 21.435000000000002 28.084999999999997 26.555 23.925 120-124 21.545 28.945 25.71 23.799999999999997 125-129 21.72 28.799999999999997 26.119999999999997 23.36 130-134 21.055 27.485 26.88 24.58 135-139 22.64 27.445000000000004 25.895000000000003 24.02 140-144 21.845 27.865000000000002 26.13 24.16 145-149 21.86 27.29 25.415 25.435000000000002 150-151 20.9 28.775000000000002 26.5875 23.7375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.5 19 1.5 20 1.0 21 1.0 22 2.5 23 2.0 24 1.5 25 5.5 26 8.0 27 9.5 28 7.0 29 7.0 30 13.0 31 22.0 32 38.0 33 56.0 34 68.0 35 84.0 36 97.5 37 104.0 38 127.0 39 142.0 40 159.0 41 182.5 42 197.5 43 231.5 44 255.5 45 245.0 46 231.0 47 233.5 48 223.5 49 204.5 50 187.0 51 160.0 52 143.5 53 124.0 54 95.5 55 88.0 56 75.5 57 43.0 58 32.5 59 27.5 60 14.0 61 12.5 62 9.5 63 2.5 64 5.0 65 7.0 66 4.0 67 1.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.5 73 1.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.3 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.375 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29129662522202 50.875 2 18.82770870337478 26.5 3 5.61278863232682 11.85 4 1.9538188277087036 5.5 5 0.7815275310834813 2.75 6 0.3552397868561279 1.5 7 0.10657193605683836 0.525 8 0.0 0.0 9 0.035523978685612786 0.22499999999999998 >10 0.035523978685612786 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCATTAATTAAGCTGCTTCATTATCACAAGAGAAACAAGAGAGGGAGA 11 0.27499999999999997 No Hit TGGCATTGTTTGCTGCACTTGCAGTGCTGTTTGCAGAACTGTTCGTATAA 9 0.22499999999999998 No Hit GTCGGGTATGGGTAATTAGGTTTCAAGTCTAGTAGGGTAGTATTCATTAC 7 0.17500000000000002 No Hit GTCCAGTCTGATTTCTGGCAAGCCAGTAGGTGTCAGCGAGAGGGCCCTCT 7 0.17500000000000002 No Hit ACTACTACTGGTTCAAGATAAGGAGCACTAGGACATTCAGCTCAAATGTT 7 0.17500000000000002 No Hit ATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATA 6 0.15 No Hit CCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 6 0.15 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 6 0.15 No Hit GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT 6 0.15 No Hit GCCCTTTTGCTTCTGCTGGATGGAGCCATCAGCTTCACCGTTAACCTCTT 6 0.15 No Hit AGGCATCCTCAATGCGGAGTTTGCGATCCTCAAGCTCAGTCTCCGTTGCA 6 0.15 No Hit CCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGA 6 0.15 No Hit AGACGATGGATTCTTTGATGGGCTGGAACTTAAAGGACTGAAGATCATAT 6 0.15 No Hit CCTGAAGCCCCTACTGTTCCTCTTTCCTCTAGCTTTCTCAAACTTCCTTC 6 0.15 No Hit GCAACGCAGCTCGGGATGTTCATGGTAGCACCTAACTGTATGGCATAGTT 6 0.15 No Hit ATATAATGAAAGACAAGATTGATTTTAAACTCTCTTAACTAAGAAGTTTT 5 0.125 No Hit AACAAATCCAAAACCCCACATTTATATATTTATATGTTATGATATGTACA 5 0.125 No Hit CTCTCTTCTCCACAGTAATCTTTCTTTTCGACTGTCTCATTTATTTCTAA 5 0.125 No Hit AGGACATGAAACCTTTTTACGTTCTTAGAATAGGTTAGTTCAAGACAATA 5 0.125 No Hit GGACAGGACAACCTAGTAAAGTGAAACCAAACTATCAAGACCAGTTTAAA 5 0.125 No Hit ATCAGATTTTGGGTTTTGGGTGAGTGAACCTCACTTGGCCATCATGACTT 5 0.125 No Hit GTAGTGTTTGGATGGCAAGAAAGTGAAGGAAAAGGTGATTTGTTTTCGAT 5 0.125 No Hit GGCCCTCCACCCACTCTGTACCCTTCAACGAATCCCATAAGCACAACCTG 5 0.125 No Hit CGAGAATATCATCAAGCGAAATATTCCCAATCTCCTTTCTGATAAGAGAT 5 0.125 No Hit GCAAAATATTAAAGAAGAAATCAATTTTTGAATAAATAGAGGAAATCCAA 5 0.125 No Hit AGAGGGAATAGTAGAAAGGAGATTCAGCTGGCGGATCTGACACTCTCAAT 5 0.125 No Hit ACGCCCTGTACACCATATTACAGAAATAGGATTACAAGCACAACAACCAT 5 0.125 No Hit CCCCTCCTTGGTAGTGGCTGCAGGTAAGGAGCTTGATCTCTGAAACTCTA 5 0.125 No Hit CTCAGGATCCTTGATTTGCTTCGGCGGGAGAAGATCCCAGTCAGTGTACA 5 0.125 No Hit GGGCTTAGACCAAGACGCAACCCTCCAAGGCACCTAAATATCAGTAACAG 5 0.125 No Hit GCACTTCTTGGATTTGAACAGAACCATCTCAACGACGAGGCATGCGGGGC 5 0.125 No Hit CTCGTCAACTCAGGAACAGCTCCAAAAACACCAATGGTTGATCTTCTTTG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACGACATCTCGTAT 5 0.125 TruSeq Adapter, Index 19 (97% over 37bp) CTCCACGCTGGGCTTTCCCTCCATGGAACAGCTCACTCTTCCCTTCTTTG 5 0.125 No Hit GGGGTGGGTCTTGGTAATCTTTTGCAGAGAATGATCCTGCCTCTGCCACT 5 0.125 No Hit CTTCCAAAGACACTCCATGATTGATCACCTGAAAAAAGCCATAGTTCTTG 5 0.125 No Hit AGCAAGCTCTTCAAATGGCACCAATGGCAGATCCTTCAATTTCCTTGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.1875 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3625 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.425 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.7250000000000001 0.0 0.0 0.0 0.0 86-87 0.8999999999999999 0.0 0.0 0.0 0.0 88-89 1.0875 0.0 0.0 0.0 0.0 90-91 1.3125 0.0 0.0 0.0 0.0 92-93 1.6375 0.0 0.0 0.0 0.0 94-95 1.8875000000000002 0.0 0.0 0.0 0.0 96-97 2.1375 0.0 0.0 0.0 0.0 98-99 2.675 0.0 0.0 0.0 0.0 100-101 3.05 0.0 0.0 0.0 0.0 102-103 3.325 0.0 0.0 0.0 0.0 104-105 3.7625 0.0 0.0 0.0 0.0 106-107 4.3625 0.0 0.0 0.0 0.0 108-109 5.0 0.0 0.0 0.0 0.0 110-111 5.449999999999999 0.0 0.0 0.0 0.0 112-113 5.8625 0.0 0.0 0.0 0.0 114-115 6.550000000000001 0.0 0.0 0.0 0.0 116-117 7.1625 0.0 0.0 0.0 0.0 118-119 8.0125 0.0 0.0 0.0 0.0 120-121 8.6875 0.0 0.0 0.0 0.0 122-123 9.575 0.0 0.0 0.0 0.0 124-125 10.175 0.0 0.0 0.0 0.0 126-127 11.024999999999999 0.0 0.0 0.0 0.0 128-129 11.9 0.0 0.0 0.0 0.0 130-131 12.925 0.0 0.0 0.0 0.0 132-133 14.125 0.0 0.0 0.0 0.0 134-135 14.9 0.0 0.0 0.0 0.0 136-137 15.962499999999999 0.0 0.0 0.0 0.0 138-139 16.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGTGG 10 0.006830828 145.0 1 GGGTGGG 10 0.006830828 145.0 2 ATACAGG 10 0.006830828 145.0 145 >>END_MODULE SRR13695419 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695419_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.32475 37.0 37.0 37.0 37.0 37.0 2 36.4485 37.0 37.0 37.0 37.0 37.0 3 36.2715 37.0 37.0 37.0 37.0 37.0 4 36.3825 37.0 37.0 37.0 37.0 37.0 5 36.4685 37.0 37.0 37.0 37.0 37.0 6 36.363 37.0 37.0 37.0 37.0 37.0 7 36.3965 37.0 37.0 37.0 37.0 37.0 8 36.445 37.0 37.0 37.0 37.0 37.0 9 36.422 37.0 37.0 37.0 37.0 37.0 10-14 36.4365 37.0 37.0 37.0 37.0 37.0 15-19 36.3812 37.0 37.0 37.0 37.0 37.0 20-24 36.378750000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.30455 37.0 37.0 37.0 37.0 37.0 30-34 36.25415 37.0 37.0 37.0 37.0 37.0 35-39 36.27335 37.0 37.0 37.0 37.0 37.0 40-44 36.27685 37.0 37.0 37.0 37.0 37.0 45-49 36.22235 37.0 37.0 37.0 37.0 37.0 50-54 36.185750000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.18835 37.0 37.0 37.0 37.0 37.0 60-64 36.18705 37.0 37.0 37.0 37.0 37.0 65-69 36.1241 37.0 37.0 37.0 37.0 37.0 70-74 36.03675 37.0 37.0 37.0 37.0 37.0 75-79 36.10545 37.0 37.0 37.0 37.0 37.0 80-84 36.09065 37.0 37.0 37.0 37.0 37.0 85-89 36.052749999999996 37.0 37.0 37.0 37.0 37.0 90-94 36.047349999999994 37.0 37.0 37.0 37.0 37.0 95-99 36.04825 37.0 37.0 37.0 37.0 37.0 100-104 36.027249999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.02265 37.0 37.0 37.0 37.0 37.0 110-114 35.900349999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.87855 37.0 37.0 37.0 37.0 37.0 120-124 35.858850000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.852850000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.64045 37.0 37.0 37.0 37.0 37.0 135-139 35.614250000000006 37.0 37.0 37.0 37.0 37.0 140-144 35.42525 37.0 37.0 37.0 34.6 37.0 145-149 35.212149999999994 37.0 37.0 37.0 32.2 37.0 150-151 34.99925 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 3.0 15 0.0 16 1.0 17 2.0 18 0.0 19 0.0 20 3.0 21 3.0 22 3.0 23 7.0 24 4.0 25 5.0 26 13.0 27 8.0 28 15.0 29 8.0 30 22.0 31 28.0 32 43.0 33 71.0 34 175.0 35 449.0 36 2847.0 37 288.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.502630919569036 19.293410172889 14.18190929591581 32.02204961162616 2 27.750000000000004 27.224999999999998 27.450000000000003 17.575 3 23.599999999999998 26.924999999999997 30.025000000000002 19.45 4 25.924999999999997 30.975 24.65 18.45 5 26.700000000000003 34.849999999999994 21.925 16.525000000000002 6 21.475 39.050000000000004 21.5 17.974999999999998 7 22.15 22.5 37.65 17.7 8 24.275 24.275 27.85 23.599999999999998 9 23.400000000000002 26.075 28.025 22.5 10-14 24.884999999999998 28.935 25.235000000000003 20.945 15-19 24.060000000000002 27.83 26.595000000000002 21.515 20-24 24.0960240060015 28.30207551887972 26.3615903975994 21.240310077519382 25-29 24.946236559139784 27.326831707926978 26.776694173543387 20.950237559389848 30-34 23.840960240060017 28.017004251062765 27.216804201050266 20.92523130782696 35-39 24.301075268817204 27.011752938234558 27.60190047511878 21.085271317829456 40-44 24.23105776444111 28.312078019504877 26.881720430107524 20.575143785946487 45-49 23.96599149787447 28.577144286071515 26.796699174793698 20.660165041260314 50-54 24.321080270067515 28.217054263565895 26.641660415103775 20.820205051262818 55-59 23.470867716929234 28.57214303575894 27.116779194798703 20.84021005251313 60-64 24.321080270067515 27.67691922980745 26.641660415103775 21.360340085021257 65-69 23.704740948189638 27.520504100820165 27.40548109621924 21.369273854770952 70-74 24.116029007251814 27.2768192048012 27.796949237309327 20.810202550637662 75-79 23.835958989747436 27.536884221055264 27.27181795448862 21.355338834708675 80-84 23.80595148787197 28.267066766691674 26.426606651662915 21.500375093773442 85-89 23.760940235058765 28.327081770442607 26.16154038509627 21.75043760940235 90-94 23.53088272068017 28.30207551887972 26.271567891972992 21.895473868467118 95-99 24.701175293823454 28.52213053263316 26.751687921980494 20.02500625156289 100-104 24.98624656164041 27.54688672168042 27.076769192298073 20.390097524381094 105-109 24.296074018504626 27.691922980745186 27.35183795948987 20.660165041260314 110-114 25.22630657664416 27.81695423855964 26.941735433858465 20.015003750937733 115-119 25.791447861965494 28.457114278569644 26.391597899474867 19.359839959989998 120-124 26.106526631657918 27.58689672418104 26.42160540135034 19.884971242810703 125-129 25.721430357589398 28.592148037009252 26.0865216304076 19.599899974993747 130-134 26.691672918229557 28.1470367591898 25.81645411352838 19.344836209052264 135-139 27.246811702925733 27.22680670167542 26.021505376344084 19.504876219054765 140-144 28.672168042010505 27.09177294323581 26.296574143535885 17.939484871217804 145-149 28.91722930732683 26.786696674168542 25.506376594148538 18.78969742435609 150-151 28.95723930982746 27.981995498874717 25.44386096524131 17.616904226056516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 1.5 10 2.5 11 1.0 12 1.0 13 1.0 14 0.0 15 0.0 16 2.0 17 2.0 18 0.0 19 0.0 20 0.5 21 1.5 22 1.5 23 1.5 24 2.5 25 1.5 26 0.0 27 3.0 28 4.0 29 3.5 30 11.0 31 13.5 32 18.5 33 26.0 34 26.0 35 34.5 36 55.5 37 77.0 38 106.0 39 142.5 40 179.5 41 236.0 42 251.0 43 229.0 44 253.0 45 284.0 46 274.5 47 261.5 48 244.5 49 217.0 50 197.0 51 167.5 52 128.0 53 107.0 54 103.5 55 88.0 56 61.5 57 42.5 58 36.0 59 30.5 60 17.0 61 8.5 62 8.0 63 5.5 64 5.0 65 4.5 66 2.5 67 0.5 68 0.0 69 1.0 70 1.0 71 1.0 72 1.0 73 0.0 74 1.0 75 1.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.02 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.025 135-139 0.025 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 72.8525980911983 51.525 2 18.487097914457408 26.150000000000002 3 5.4789678331565925 11.625 4 1.8734535171438673 5.3 5 0.7423117709437964 2.625 6 0.2827854365500177 1.2 7 0.17674089784376104 0.8750000000000001 8 0.03534817956875221 0.2 9 0.03534817956875221 0.22499999999999998 >10 0.03534817956875221 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCCACAACAATATCTTGACCAGCCTTAAATTTCATTAGGGCTCATT 11 0.27499999999999997 No Hit ATGTCCAGGGAGAGATTGCGCTACTTAGAAGCTTTGGCAATTTATTGTGA 9 0.22499999999999998 No Hit TGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTA 8 0.2 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 7 0.17500000000000002 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 7 0.17500000000000002 No Hit CAATATTGCAAATAGATTGGATTACGACCCTAGTTCTATTCTCAAAGTTT 7 0.17500000000000002 No Hit GAGCTGAGTTCCAAGCCAGCGATCTTGGTTTAAGCATTGAAAACACATCA 7 0.17500000000000002 No Hit CTGGCAGCTTGGATTGAGGTAATTTGGAACCCATTTTTTCTTCATCTGCC 7 0.17500000000000002 No Hit GTTTACAGAGACAGCTAGGGCCAGGATTGAGAAGGCTGGAGGAGAGTGCT 6 0.15 No Hit AAAAAGATCAACCTTCTTTCATTCTCATCCATCGGTTGAATAAGTTACCG 6 0.15 No Hit CTCATCGTTGACCAGATGATAACGTGGGACATTATCTTTGGGGCAATACC 6 0.15 No Hit CAGCAAAAAATGTCTCAAGGCTCTGCCTACCAAACTAGGCAGCGGCAAGG 6 0.15 No Hit ATCTCATCGGCAGCCGTTGCCACCGTCAACCGCACCCCGGTACAAGCCAA 6 0.15 No Hit CTATAAACCTCAAATCCTCTTTCTTGGATCACAAATCATCCTTCCATGGC 6 0.15 No Hit CACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGG 6 0.15 No Hit CTGCTTTGGTATCCTTTGTAGCAGTGCTCAGTCCTGACTCAAGCTTCTTC 6 0.15 No Hit CAACTGGTGCTGCTGCTATGACAGGAAAATTGCAGGCTTTTAATCAGAAT 5 0.125 No Hit CGATATTGGAAGTCTTGTTTAGTGGAGCAAGGTTGGGATGGAAGGACCAC 5 0.125 No Hit TCGCTGTTCTCATGTCATCTATCATTTTTTCGACTTCATCGATGAGAGAG 5 0.125 No Hit CCACTAGTGAGTGTATTGTCATTGAAGATGCACTAGCTGGAGTACAGGCT 5 0.125 No Hit GTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCAC 5 0.125 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 5 0.125 No Hit TGTTGAGGTTAATACAAAGCTTAGTGAAACTCCTGGACTGATAAATAAAA 5 0.125 No Hit CTTCATTTCTGCTGCTGAGTTGCGTCATGTCATGACAAATCTCGGGGAGA 5 0.125 No Hit CAAAGGAAGCTAAGCAGTTGGAATTGAAGGATAGCAAGGTTGTGACAGAA 5 0.125 No Hit CAAGAGGCCAAAACCTCTCTTCTTCTGTCAACCCTTCTTTGAAGAACTTG 5 0.125 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 5 0.125 No Hit TCATGTTATACTCACTGGTTCTTACAGATAGTCCCTCAATTAAGTGGGGT 5 0.125 No Hit GCTATGTTAGGCCGGAATCTGAACGTCCCCGGCTATGGGAAGTTTCAACG 5 0.125 No Hit GTTAAAGTTAGCGGGGTGAAGATAAGCCCGAATCCGGTGAAGAAAGGGAA 5 0.125 No Hit ATTGAGAGAGGAACTTTCTATGGTTAGATCCCTACATCCTAGAGGCCTGG 5 0.125 No Hit CGATACAGAGAGTTTGGGATCCCAACTAATTGGTTATTAGATTCAGGAGT 5 0.125 No Hit CCCGTACAGTATCATGTTTGGACCAGATATCTGTGGACACAGCACCAAGA 5 0.125 No Hit TGATGATGTTGTGTTCTTAAAACTTGATTGCAACCAAGAAAACAAGCCGT 5 0.125 No Hit GTCAAGTTCGGTGAGTCAGTTTGGTTCAAGGCTGGAGCTCAAATCTTCTC 5 0.125 No Hit GAATTCTGAAAGCATATGGTTGTATTGGTTTTTAATCTGTGGGTTTACAG 5 0.125 No Hit GGCACGTGAAGTTGAAAAGCTACGTGTAGAGCTTTCTAGTTCTGATAATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.23750000000000002 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.325 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.4625 0.0 0.0 0.0 0.0 84-85 0.6625 0.0 0.0 0.0 0.0 86-87 0.8500000000000001 0.0 0.0 0.0 0.0 88-89 1.0375 0.0 0.0 0.0 0.0 90-91 1.2625 0.0 0.0 0.0 0.0 92-93 1.5875 0.0 0.0 0.0 0.0 94-95 1.8375 0.0 0.0 0.0 0.0 96-97 2.0875 0.0 0.0 0.0 0.0 98-99 2.625 0.0 0.0 0.0 0.0 100-101 3.0 0.0 0.0 0.0 0.0 102-103 3.2750000000000004 0.0 0.0 0.0 0.0 104-105 3.7125000000000004 0.0 0.0 0.0 0.0 106-107 4.3125 0.0 0.0 0.0 0.0 108-109 4.9125 0.0 0.0 0.0 0.0 110-111 5.324999999999999 0.0 0.0 0.0 0.0 112-113 5.7375 0.0 0.0 0.0 0.0 114-115 6.449999999999999 0.0 0.0 0.0 0.0 116-117 7.05 0.0 0.0 0.0 0.0 118-119 7.9 0.0 0.0 0.0 0.0 120-121 8.6125 0.0 0.0 0.0 0.0 122-123 9.5 0.0 0.0 0.0 0.0 124-125 10.100000000000001 0.0 0.0 0.0 0.0 126-127 10.95 0.0 0.0 0.0 0.0 128-129 11.725000000000001 0.0 0.0 0.0 0.0 130-131 12.6875 0.0 0.0 0.0 0.0 132-133 13.875 0.0 0.0 0.0 0.0 134-135 14.65 0.0 0.0 0.0 0.0 136-137 15.7 0.0 0.0 0.0 0.0 138-139 16.487499999999997 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACATT 10 0.006830828 145.0 145 >>END_MODULE Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra Read 707483 spots for SRR13695419.sra Written 707483 spots for SRR13695419.sra Read 707466 spots for SRR13695419.sra Written 707466 spots for SRR13695419.sra SRR ids: ['SRR13695419.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6fg7gglh SRR13695419.sra spots: 14149337 blocks: [[1, 707466], [707467, 1414932], [1414933, 2122398], [2122399, 2829864], [2829865, 3537330], [3537331, 4244796], [4244797, 4952262], [4952263, 5659728], [5659729, 6367194], [6367195, 7074660], [7074661, 7782126], [7782127, 8489592], [8489593, 9197058], [9197059, 9904524], [9904525, 10611990], [10611991, 11319456], [11319457, 12026922], [12026923, 12734388], [12734389, 13441854], [13441855, 14149337]] SRR13695419 file size 4786863 SRR13695419 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695419 SRR13695419_1.fastq SRR13695419_2.fastq Input file: SRR13695419_1.fastq Paired file: SRR13695419_2.fastq trimmed: SRR13695419-trimmed-pair1.fastq, SRR13695419-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:51:21 2025 >> started Wed Feb 12 01:51:36 2025 >> done (15.205s) 14149337 read pairs processed; of these: 103 ( 0.00%) short read pairs filtered out after trimming by size control 15586 ( 0.11%) empty read pairs filtered out after trimming by size control 14133648 (99.89%) read pairs available; of these: 3153003 (22.31%) trimmed read pairs available after processing 10980645 (77.69%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 0 0.00% 23 1 0.00% 24 3 0.00% 25 2 0.00% 26 2 0.00% 27 5 0.00% 28 1 0.00% 29 6 0.00% 30 4 0.00% 31 5 0.00% 32 3 0.00% 33 2 0.00% 34 10 0.00% 35 25 0.00% 36 13 0.00% 37 23 0.00% 38 16 0.00% 39 20 0.00% 40 28 0.00% 41 35 0.00% 42 43 0.00% 43 40 0.00% 44 37 0.00% 45 42 0.00% 46 77 0.00% 47 85 0.00% 48 118 0.00% 49 125 0.00% 50 142 0.00% 51 153 0.00% 52 170 0.00% 53 219 0.00% 54 262 0.00% 55 274 0.00% 56 303 0.00% 57 376 0.00% 58 445 0.00% 59 533 0.00% 60 561 0.00% 61 704 0.00% 62 836 0.01% 63 927 0.01% 64 1051 0.01% 65 1137 0.01% 66 1257 0.01% 67 1470 0.01% 68 1704 0.01% 69 1818 0.01% 70 2224 0.02% 71 2491 0.02% 72 2880 0.02% 73 3426 0.02% 74 3741 0.03% 75 4435 0.03% 76 4686 0.03% 77 5140 0.04% 78 5840 0.04% 79 6457 0.05% 80 7135 0.05% 81 8063 0.06% 82 9128 0.06% 83 10105 0.07% 84 11219 0.08% 85 12073 0.09% 86 13162 0.09% 87 14244 0.10% 88 15156 0.11% 89 16126 0.11% 90 17119 0.12% 91 18050 0.13% 92 19034 0.13% 93 20848 0.15% 94 22052 0.16% 95 23735 0.17% 96 25339 0.18% 97 26635 0.19% 98 28026 0.20% 99 28643 0.20% 100 29438 0.21% 101 30427 0.22% 102 31457 0.22% 103 32226 0.23% 104 34554 0.24% 105 36126 0.26% 106 37602 0.27% 107 38808 0.27% 108 39997 0.28% 109 41515 0.29% 110 41796 0.30% 111 42792 0.30% 112 43837 0.31% 113 44630 0.32% 114 45801 0.32% 115 47646 0.34% 116 48557 0.34% 117 50307 0.36% 118 51159 0.36% 119 51636 0.37% 120 52278 0.37% 121 53286 0.38% 122 53427 0.38% 123 53364 0.38% 124 54910 0.39% 125 55936 0.40% 126 57416 0.41% 127 58579 0.41% 128 58861 0.42% 129 59262 0.42% 130 60614 0.43% 131 60890 0.43% 132 60537 0.43% 133 61360 0.43% 134 61770 0.44% 135 62646 0.44% 136 62636 0.44% 137 63283 0.45% 138 64961 0.46% 139 66005 0.47% 140 65668 0.46% 141 66563 0.47% 142 67273 0.48% 143 67503 0.48% 144 67589 0.48% 145 68074 0.48% 146 67669 0.48% 147 68476 0.48% 148 69482 0.49% 149 69860 0.49% 150 70188 0.50% 151 10980645 77.69% 14133648 reads passed initial QC criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.99 fanout-score-rank=13 prefix-density=0.49 prefix-fanout=2.6 sequence=CTGATGCACTGCACTTGACG criterion=fanout-score sequence-density=0.09 sequence-density-rank=26 fanout-score=15.09 fanout-score-rank=1 prefix-density=0.80 prefix-fanout=1.6 sequence=TGCTTGCTTCTAATCTTAAGGGCGCCCACAATTACGCTTGTAAGGATTTGGGCAACCTGCTGGTTTATAGGTGGGGATGGGATCTCGCTCGGCCAATTCTCGAGCAGTGCAAATGGATAAGGGAGAGAAGAC criterion=sequence-density sequence-density=0.66 sequence-density-rank=1 fanout-score=2.84 fanout-score-rank=16 prefix-density=0.74 prefix-fanout=2.5 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCATTGTTATGTATTGGCCATGTCTGTGGCCTCTGGTGTGGTTTTAATGAAGTCTTATAATTAGTGT criterion=fanout-score sequence-density=0.05 sequence-density-rank=32 fanout-score=61.01 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=8.7 sequence=AAGGGAAAGGGTGTGTACCAATATGTCGACAAATATGGTGCTAATGT SRR13695419 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:52:47 Started mapping on | Feb 12 01:52:47 Finished on | Feb 12 01:56:21 Mapping speed, Million of reads per hour | 237.76 Number of input reads | 14133648 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 12199314 Uniquely mapped reads % | 86.31% Average mapped length | 288.30 Number of splices: Total | 10361403 Number of splices: Annotated (sjdb) | 10140406 Number of splices: GT/AG | 10145470 Number of splices: GC/AG | 173498 Number of splices: AT/AC | 7915 Number of splices: Non-canonical | 34520 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.96 Insertion rate per base | 0.02% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 329058 % of reads mapped to multiple loci | 2.33% Number of reads mapped to too many loci | 73919 % of reads mapped to too many loci | 0.52% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.69% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1605434 1605434 1605434 N_multimapping 329058 329058 329058 N_noFeature 312559 11878341 450192 N_ambiguous 264050 1177 79972 UnstrandedReadsAssigned:11622705 PositiveStrandReadsAssigned:319796 NegativeStrandReadsAssigned:11669150 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=147 echo kmer=143 SRR13695419 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695419-trimmed-pair1.fastq SRR13695419-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,133,648 reads, 11,760,112 reads pseudoaligned [quant] estimated average fragment length: 211.819 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,330 rounds 52401 SRR13695419.ke.tsv 34699 SRR13695419.se.tsv 87100 total ==> SRR13695419.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.18 458 16.4279 Potri.005G024800.1.v4.1 1035 824.181 359 28.2351 Potri.004G059700.1.v4.1 961 750.2 2 0.172811 Potri.007G009000.2.v4.1 1416 1205.18 0 0 Potri.003G141000.2.v4.1 2943 2732.18 428.108 10.1569 Potri.016G087400.1.v4.1 270 97.8774 827.357 547.934 Potri.015G069301.1.v4.1 564 356.257 0 0 Potri.010G195200.1.v4.1 1773 1562.18 52 2.15769 Potri.012G127500.1.v4.1 977 766.2 314 26.5647 ==> SRR13695419.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 85 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 327 Potri.001G212900.v4.1 20 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR13695419 completed mapping pipeline successfully