Starting /dee2/code/volunteer_pipeline.sh SRR13695420 current disk space = 3050789191680 free memory = 1579070016 SRR13695420 SRAfilesize 40e73a0107e1e112b4b4ca8417c4aff3 SRR13695420.sra SRR13695420.sra file validated SRR13695420 is paired end SRR13695420 is conventional basespace SRR13695420 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695420_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6325 37.0 37.0 37.0 37.0 37.0 2 36.17375 37.0 37.0 37.0 37.0 37.0 3 36.57 37.0 37.0 37.0 37.0 37.0 4 36.6285 37.0 37.0 37.0 37.0 37.0 5 36.518 37.0 37.0 37.0 37.0 37.0 6 36.6285 37.0 37.0 37.0 37.0 37.0 7 36.5145 37.0 37.0 37.0 37.0 37.0 8 36.5775 37.0 37.0 37.0 37.0 37.0 9 36.5405 37.0 37.0 37.0 37.0 37.0 10-14 36.585 37.0 37.0 37.0 37.0 37.0 15-19 36.527300000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.512699999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.478 37.0 37.0 37.0 37.0 37.0 30-34 36.3707 37.0 37.0 37.0 37.0 37.0 35-39 36.3929 37.0 37.0 37.0 37.0 37.0 40-44 36.389300000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.3313 37.0 37.0 37.0 37.0 37.0 50-54 36.3155 37.0 37.0 37.0 37.0 37.0 55-59 36.2591 37.0 37.0 37.0 37.0 37.0 60-64 36.308499999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.2466 37.0 37.0 37.0 37.0 37.0 70-74 36.2431 37.0 37.0 37.0 37.0 37.0 75-79 36.2291 37.0 37.0 37.0 37.0 37.0 80-84 36.155100000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.1119 37.0 37.0 37.0 37.0 37.0 90-94 36.061 37.0 37.0 37.0 37.0 37.0 95-99 36.067400000000006 37.0 37.0 37.0 37.0 37.0 100-104 36.1109 37.0 37.0 37.0 37.0 37.0 105-109 36.0493 37.0 37.0 37.0 37.0 37.0 110-114 35.981199999999994 37.0 37.0 37.0 37.0 37.0 115-119 36.035199999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.966 37.0 37.0 37.0 37.0 37.0 125-129 35.9432 37.0 37.0 37.0 37.0 37.0 130-134 35.8685 37.0 37.0 37.0 37.0 37.0 135-139 35.851 37.0 37.0 37.0 37.0 37.0 140-144 35.796800000000005 37.0 37.0 37.0 37.0 37.0 145-149 35.635099999999994 37.0 37.0 37.0 37.0 37.0 150-151 35.42375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 3.0 23 2.0 24 2.0 25 1.0 26 7.0 27 8.0 28 15.0 29 17.0 30 23.0 31 45.0 32 49.0 33 84.0 34 136.0 35 359.0 36 2897.0 37 350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.35 12.0 8.3 42.35 2 19.330480745028947 13.566574377045054 36.194311603322426 30.908633274603574 3 17.325 18.325 27.175 37.175000000000004 4 21.575 23.625 25.724999999999998 29.075 5 23.549999999999997 29.599999999999998 24.575 22.275 6 21.525 34.449999999999996 22.775000000000002 21.25 7 15.5 29.75 38.0 16.75 8 17.075000000000003 27.0 32.4 23.525 9 18.099999999999998 22.475 35.775 23.65 10-14 19.355 29.5 28.349999999999998 22.795 15-19 20.1 29.42 26.625 23.855 20-24 19.439999999999998 28.585 27.615000000000002 24.36 25-29 20.305 28.58 27.034999999999997 24.08 30-34 19.615 29.035 28.044999999999998 23.305 35-39 19.89 29.330000000000002 26.945000000000004 23.835 40-44 19.52 28.655 27.88 23.945 45-49 20.53 28.615000000000002 27.400000000000002 23.455000000000002 50-54 20.064999999999998 28.785 27.63 23.52 55-59 19.814999999999998 28.42 27.445000000000004 24.32 60-64 20.215 28.299999999999997 27.515 23.97 65-69 20.5 28.985 27.62 22.895 70-74 19.975 29.255 27.339999999999996 23.43 75-79 20.015 27.415 28.09 24.48 80-84 19.650000000000002 27.925 27.98 24.445 85-89 20.835 27.800000000000004 27.145000000000003 24.22 90-94 20.385 28.37 27.18 24.065 95-99 20.635 27.775 27.800000000000004 23.79 100-104 21.095 29.12 26.965 22.82 105-109 20.93 28.405 27.3 23.365 110-114 20.87 27.83 27.72 23.580000000000002 115-119 20.955 28.275 27.07 23.7 120-124 20.825 28.925 26.35 23.9 125-129 20.724999999999998 27.985 27.0 24.29 130-134 21.085 27.605 27.215 24.095 135-139 21.355 28.685 25.8 24.16 140-144 21.545 28.265 26.775 23.415 145-149 21.165 28.89 25.575 24.37 150-151 22.0625 28.4375 25.6125 23.8875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.5 19 3.0 20 0.5 21 1.0 22 1.0 23 0.0 24 2.0 25 5.5 26 6.5 27 5.0 28 5.5 29 7.5 30 15.5 31 31.0 32 32.5 33 35.5 34 64.5 35 79.0 36 90.0 37 126.5 38 137.0 39 148.5 40 173.5 41 199.5 42 248.0 43 280.0 44 247.5 45 235.0 46 251.5 47 224.0 48 207.5 49 200.0 50 171.0 51 156.0 52 130.0 53 96.5 54 88.5 55 78.0 56 68.5 57 47.5 58 34.5 59 26.5 60 10.0 61 5.0 62 5.0 63 3.0 64 1.0 65 3.0 66 2.5 67 0.5 68 0.5 69 0.5 70 0.5 71 0.5 72 1.0 73 0.5 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.675 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 69.04761904761905 45.675 2 18.669690098261526 24.7 3 7.747543461829176 15.375 4 2.8344671201814062 7.5 5 0.944822373393802 3.125 6 0.26455026455026454 1.05 7 0.1889644746787604 0.8750000000000001 8 0.15117157974300832 0.8 9 0.15117157974300832 0.8999999999999999 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATGATGCTGCCAACATATTCACATCTGTCAGGATACATACTCATTATA 9 0.22499999999999998 No Hit CCTCATTTCCTCTTCAATGTTAACATCGTGCAGTGTAGTCTCCTCACACT 9 0.22499999999999998 No Hit GTCCAACACTGCGACAACACGCTCGCAACCTTAAAAAGTTCACCCATGTT 9 0.22499999999999998 No Hit CCAGGGACTGTCACAAGAAGGGTACGGTATGCCTGGCGGTTAGCCTCGGT 9 0.22499999999999998 No Hit CTCGTTATCATACCAAGCAATCACCTTAACCATGTCGTCTCCCATCACCA 8 0.2 No Hit CAGGTTAGGTATGAATCGAATCAATGGAAAGCTACCAGAACGGGGTTAGA 8 0.2 No Hit GCCAGTAATTGTGCTGCATTGCTAGGGGTTCCCAAGATACCATTTATACA 8 0.2 No Hit ATCGGCACCAGCAACAATCTCCAATATTTCTCCAGTAATCTTGGCCTGAC 8 0.2 No Hit GACCGAACTCCTGAGCAGAGACCACATGAGAAATTACCAGAATTACGAGG 7 0.17500000000000002 No Hit TACTTTTCTCACACCAGTCTCTCCTTCAGAAGCCAGTGAAAAGACCACTG 7 0.17500000000000002 No Hit AGGAAATCTGCTGACTCACAGACAGGACCAACCACATCAAAAGTTGAAAC 7 0.17500000000000002 No Hit CCATCAATCTGGCTCTTGTAACCAATAACAGTCAAAACAGTGATATAAAG 7 0.17500000000000002 No Hit CAACAACCGAAGAATCAAGATACTCCTGCAAGCCCTCCAGATCATCAAAG 7 0.17500000000000002 No Hit GCTGCCGTGCCCGTTGGAGCTCCTGTTCCAGTTGGCTGAGCTTCAAGCGG 6 0.15 No Hit CCTCTCTTCCCATTACTCTTTGCACCCTCACCATCATTTCCGTTACCAAT 6 0.15 No Hit GCTACTTTCTACAGATGTTCTAGTGGATTAGGAATAAGCTCCATGATGAA 6 0.15 No Hit TTCTCGGTGAACTCTTCCTCTAACCACTTGTGATCCTTGATAACCTCCTT 6 0.15 No Hit CTCTGCACTGCCCTCCTCATCATCTTCATCTTCATCATTGACTTCCGACA 6 0.15 No Hit GCCTGCTTTTCACGGCGCTTGTAGTTTGTAAATGTTATTACAGAAAGCAA 6 0.15 No Hit GCTCTGCCAAGGGTGATCCATGCCTCCGCCCATGACGATTCCAACTCAGT 6 0.15 No Hit CCCACGACCGTCTCAGCAGCTCCCGCAAAGTGACCCTTCTCTGGTGCCAC 5 0.125 No Hit GTGCTTCTCAACAGTAGTCCAAACCTTGGATACCCAAGGGCAAGTTGTAT 5 0.125 No Hit GTAACGTCAGCATGGATGGCCTCATCCAGCATGCGAGAGAGACAGCGAAG 5 0.125 No Hit GCCATGTTCAACAAGATGACACCCAGGTCTGTTTGTTTCTCTAGCAACGA 5 0.125 No Hit CCATTCTCCTGATACCCAAGAACAACTAAATTAATAATACCAAAATTTAA 5 0.125 No Hit ACAACACTCCATCCATATCAAAAAGCACAGCAGATACCTTACCCCACTCA 5 0.125 No Hit CGGGATTCCACGTGCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGAT 5 0.125 No Hit GGATTAGACTCTAAATTCTGTTTTAAAAAGTCCCTTGATGGGGCTTCCTT 5 0.125 No Hit GCAACTCTTGCGCAACTCGAGTTACTTCTTCTTGTTGTTCTTTAGTTAGC 5 0.125 No Hit TCATAATTCACAATTTTCAGTGAGTTCAAAGTCGAGCAGAGCGTTTTACT 5 0.125 No Hit CTCCACATTCCACTCCTCAGCAACAGCCTTCAATTCATCCACATCCACCT 5 0.125 No Hit GATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAA 5 0.125 No Hit TTATTGTTTTAAAATAGAAAGTACAAATATAAATATAAAAAGAACAAACA 5 0.125 No Hit TGGGAATTTAATGTGCAGGGTGACCTCGATCATAAATGAAGCACGAAGTG 5 0.125 No Hit ATGGATTTGAACTCTTGAAAGAGTTTGTAACCTCAGAATAATTTGATGAA 5 0.125 No Hit CTGCACAATAGCTCTCCCCCTCTTAACAGGGTCCCCACTCTTAAAAACCC 5 0.125 No Hit CCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCT 5 0.125 No Hit GTCCATTCTGTGTCCAGGCAGCAAATCCACCAGCAATGTCAGTCACTGCA 5 0.125 No Hit TTTTTTTTTTTTGCAAAAGTTATAATTGAAATGTGAAAACATCGTTCAAA 5 0.125 No Hit CTCTCACTTTCCGGGGGCGAAGTTTGTGGCATATGCCCAGGCGTTGTTGT 5 0.125 No Hit CTTGCGGATATACTCAAAAAGCTCTAAAATGGCAGAGTTCAGCAGATTAT 5 0.125 No Hit GTTGTAATCATTCCAGTGTCACCGATGCATTCAAATGAGTAATCTGCCCC 5 0.125 No Hit GGGTAAGCTTTCTTTGCCTCCTCAAGCTCAAGCAACACTTGAGATGCCTC 5 0.125 No Hit GTTTCTATAACCTTGGAGCTAGGCGATTGAGAGGATGGTCCCAATCGGCT 5 0.125 No Hit CCTAAAAGTAATCCTGAAAGAACAAAATGATGCACTTAAAACAGAGATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.5 0.0 0.0 0.0 0.0 90-91 0.6 0.0 0.0 0.0 0.0 92-93 0.7749999999999999 0.0 0.0 0.0 0.0 94-95 0.975 0.0 0.0 0.0 0.0 96-97 1.1375000000000002 0.0 0.0 0.0 0.0 98-99 1.3125 0.0 0.0 0.0 0.0 100-101 1.9125 0.0 0.0 0.0 0.0 102-103 2.2875 0.0 0.0 0.0 0.0 104-105 2.45 0.0 0.0 0.0 0.0 106-107 2.7125 0.0 0.0 0.0 0.0 108-109 3.175 0.0 0.0 0.0 0.0 110-111 3.6125 0.0 0.0 0.0 0.0 112-113 4.0375 0.0 0.0 0.0 0.0 114-115 4.5625 0.0 0.0 0.0 0.0 116-117 5.0 0.0 0.0 0.0 0.0 118-119 5.275 0.0 0.0 0.0 0.0 120-121 5.7875 0.0 0.0 0.0 0.0 122-123 6.475 0.0 0.0 0.0 0.0 124-125 6.825 0.0 0.0 0.0 0.0 126-127 7.5 0.0 0.0 0.0 0.0 128-129 8.2125 0.0 0.0 0.0 0.0 130-131 8.6875 0.0 0.0 0.0 0.0 132-133 9.6375 0.0 0.0 0.0 0.0 134-135 10.4625 0.0 0.0 0.0 0.0 136-137 11.35 0.0 0.0 0.0 0.0 138-139 11.837499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGAC 10 0.006830828 145.0 3 GGTTCCC 10 0.006830828 145.0 145 ACTCTAA 10 0.006830828 145.0 8 ATATGGC 10 0.006830828 145.0 7 AGACTCT 10 0.006830828 145.0 6 GACTCTA 10 0.006830828 145.0 7 AGTTAAT 10 0.006830828 145.0 2 TATGGCA 10 0.006830828 145.0 8 >>END_MODULE SRR13695420 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695420_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.98 37.0 37.0 37.0 37.0 37.0 2 36.164 37.0 37.0 37.0 37.0 37.0 3 36.198 37.0 37.0 37.0 37.0 37.0 4 36.184 37.0 37.0 37.0 37.0 37.0 5 36.25 37.0 37.0 37.0 37.0 37.0 6 36.292 37.0 37.0 37.0 37.0 37.0 7 36.2685 37.0 37.0 37.0 37.0 37.0 8 36.375 37.0 37.0 37.0 37.0 37.0 9 36.289 37.0 37.0 37.0 37.0 37.0 10-14 36.275 37.0 37.0 37.0 37.0 37.0 15-19 36.2681 37.0 37.0 37.0 37.0 37.0 20-24 36.1976 37.0 37.0 37.0 37.0 37.0 25-29 36.1267 37.0 37.0 37.0 37.0 37.0 30-34 36.0654 37.0 37.0 37.0 37.0 37.0 35-39 36.031400000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.0447 37.0 37.0 37.0 37.0 37.0 45-49 36.0477 37.0 37.0 37.0 37.0 37.0 50-54 36.013400000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.9535 37.0 37.0 37.0 37.0 37.0 60-64 35.9234 37.0 37.0 37.0 37.0 37.0 65-69 35.9056 37.0 37.0 37.0 37.0 37.0 70-74 35.8533 37.0 37.0 37.0 37.0 37.0 75-79 35.852500000000006 37.0 37.0 37.0 37.0 37.0 80-84 35.8618 37.0 37.0 37.0 37.0 37.0 85-89 35.73440000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.7749 37.0 37.0 37.0 37.0 37.0 95-99 35.726800000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.7296 37.0 37.0 37.0 37.0 37.0 105-109 35.71249999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.6229 37.0 37.0 37.0 37.0 37.0 115-119 35.563 37.0 37.0 37.0 37.0 37.0 120-124 35.5198 37.0 37.0 37.0 37.0 37.0 125-129 35.514799999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.4486 37.0 37.0 37.0 37.0 37.0 135-139 35.2829 37.0 37.0 37.0 34.6 37.0 140-144 35.1509 37.0 37.0 37.0 29.8 37.0 145-149 34.959700000000005 37.0 37.0 37.0 25.0 37.0 150-151 34.692375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 4.0 13 8.0 14 4.0 15 3.0 16 3.0 17 3.0 18 2.0 19 2.0 20 0.0 21 3.0 22 0.0 23 4.0 24 2.0 25 5.0 26 10.0 27 14.0 28 9.0 29 22.0 30 28.0 31 42.0 32 61.0 33 98.0 34 210.0 35 578.0 36 2632.0 37 251.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.9466800804829 23.516096579476862 11.066398390342053 28.470824949698187 2 25.825 26.400000000000002 31.025000000000002 16.75 3 21.925 26.8 32.025 19.25 4 25.0 32.7 22.55 19.75 5 26.575 37.25 20.349999999999998 15.825 6 21.15 37.974999999999994 21.475 19.400000000000002 7 20.95 23.075000000000003 37.7 18.275 8 20.875 26.55 28.875 23.7 9 23.150000000000002 23.3 31.8 21.75 10-14 24.099999999999998 28.9 26.595000000000002 20.405 15-19 23.905 27.925 27.950000000000003 20.22 20-24 23.431715857928964 28.429214607303656 27.253626813406704 20.885442721360683 25-29 23.051525762881443 28.159079539769884 27.698849424712357 21.09054527263632 30-34 22.826413206603302 28.124062031015505 27.85892946473237 21.190595297648823 35-39 23.621810905452726 28.829414707353678 27.168584292146075 20.380190095047524 40-44 23.206603301650823 29.164582291145575 26.898449224612307 20.730365182591296 45-49 23.02151075537769 27.71385692846423 28.29414707353677 20.97048524262131 50-54 23.19159579789895 28.264132066033014 27.358679339669834 21.1855927963982 55-59 23.506753376688344 27.1935967983992 27.593796898449224 21.70585292646323 60-64 23.47173586793397 27.99899949974988 27.56378189094547 20.965482741370685 65-69 23.159263705482193 27.39095638255302 28.07623049219688 21.373549419767908 70-74 23.85192596298149 28.354177088544276 26.788394197098548 21.005502751375687 75-79 23.36168084042021 28.14407203601801 27.658829414707352 20.83541770885443 80-84 23.121560780390197 28.46423211605803 27.178589294647328 21.235617808904454 85-89 22.681340670335167 27.5687843921961 28.644322161080538 21.105552776388194 90-94 24.192096048024013 28.53926963481741 26.29814907453727 20.97048524262131 95-99 23.461730865432717 28.284142071035518 27.46873436718359 20.785392696348172 100-104 23.486743371685844 28.119059529764883 27.238619309654826 21.155577788894448 105-109 23.816908454227114 28.3991995997999 27.27863931965983 20.505252626313155 110-114 24.14207103551776 27.373686843421712 27.818909454727365 20.665332666333168 115-119 24.592296148074038 28.019009504752372 27.073536768384194 20.315157578789396 120-124 25.067533766883443 28.844422211105552 26.253126563281644 19.834917458729365 125-129 25.352676338169083 28.174087043521762 26.718359179589797 19.754877438719358 130-134 26.385831498899336 27.331398839303585 27.506503902341407 18.776265759455672 135-139 26.4032016008004 27.978989494747374 26.703351675837915 18.914457228614307 140-144 27.64382191095548 28.14407203601801 25.652826413206604 18.55927963981991 145-149 27.813906953476735 27.178589294647328 26.488244122061033 18.519259629814908 150-151 27.82989368355222 27.40462789243277 26.52908067542214 18.23639774859287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 1.0 5 1.5 6 1.5 7 1.0 8 0.5 9 1.0 10 0.5 11 0.5 12 0.5 13 0.0 14 1.0 15 1.5 16 0.5 17 0.5 18 1.0 19 0.5 20 0.0 21 1.0 22 1.5 23 2.5 24 3.5 25 2.5 26 2.0 27 4.0 28 10.0 29 13.5 30 13.5 31 22.5 32 27.0 33 30.0 34 42.5 35 72.5 36 97.5 37 113.5 38 127.0 39 143.5 40 173.0 41 212.0 42 230.5 43 252.0 44 265.5 45 259.5 46 269.5 47 253.5 48 242.5 49 222.0 50 182.0 51 155.5 52 128.5 53 93.5 54 72.5 55 67.0 56 43.5 57 28.5 58 25.5 59 19.5 60 16.0 61 9.0 62 5.5 63 4.5 64 4.5 65 1.5 66 1.0 67 1.0 68 0.5 69 1.5 70 1.0 71 0.5 72 1.0 73 0.5 74 0.0 75 0.5 76 1.0 77 0.5 78 0.5 79 0.5 80 0.5 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.05 30-34 0.05 35-39 0.05 40-44 0.05 45-49 0.05 50-54 0.05 55-59 0.05 60-64 0.05 65-69 0.04 70-74 0.05 75-79 0.05 80-84 0.05 85-89 0.05 90-94 0.05 95-99 0.05 100-104 0.05 105-109 0.05 110-114 0.05 115-119 0.05 120-124 0.05 125-129 0.05 130-134 0.06 135-139 0.05 140-144 0.05 145-149 0.05 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.375 #Duplication Level Percentage of deduplicated Percentage of total 1 69.22787193973635 45.95 2 18.794726930320152 24.95 3 7.269303201506591 14.475 4 3.0131826741996233 8.0 5 1.0169491525423728 3.375 6 0.18832391713747645 0.75 7 0.22598870056497175 1.05 8 0.18832391713747645 1.0 9 0.07532956685499058 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCACCTTCCAATCTTAACCATCCACCCCATCTCCCCGGCACCAGAAAA 9 0.22499999999999998 No Hit ATTGACACACCTTGATGGCAGGCAGCTTCTTATCAAATCTAACCCTGGTG 9 0.22499999999999998 No Hit AGATGGCCTCGGCATCATTTCTCAAGTCATCACCAGTTCTAGACAAGTCT 8 0.2 No Hit GAAATTGACAAGGCTCTTGGTCATTCTTAGGGCATCATAGGACATGAGTA 8 0.2 No Hit CCAAGTTTTCCTTCTTGTCGACTAGTCATATTTCCTAGCTAACACCTGAA 8 0.2 No Hit CACCAATTTTACAGTTTGAGCAGGATCCCGTTCAGATTCTTGATGCCTTG 8 0.2 No Hit GAGAAACAATTAAATGAAGGGATTTGCATTGATGGTTTGATGTTAACTTA 8 0.2 No Hit CATGGATTTCTCTTCCCAAGGGCAAGGGTATTAAGTTGTCTATCATCGAG 7 0.17500000000000002 No Hit GAGAAGAAAGCAGAGCCCGAGCCTTCTCATGAGATTTTGACAAACCCAGC 7 0.17500000000000002 No Hit CTGGGGCACTAATCTGGGTATTCACGACCTCAATTTCGGATATACTCATG 7 0.17500000000000002 No Hit AGGCTTTCAGTTCAAGCTGGAGCAGCAGGGATTATTGTGTCGAATCACGG 7 0.17500000000000002 No Hit CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 7 0.17500000000000002 No Hit AAACTAATGGAACAAAAAATTTTGTTGTGATAGATGGAAGCATGGCTGAA 7 0.17500000000000002 No Hit CATCGTCCTCGTAAAAACATGTTCATGTTTCAGAAATGGGGATTTGACAA 6 0.15 No Hit GAAGGGTTCCTACAGGAGGGTGGTTCAGAAAACATGAATTTATTCAGCTA 6 0.15 No Hit GACAAATTGAAGAAGAAACTCTATTTGGAAGCACAGGCTCGAGTTTATTC 6 0.15 No Hit CCTTTGTTTTCTTTTTATTATTACTATTACTATTATTATTATTTTTTGTG 6 0.15 No Hit ACCAAGAGCGCTAAGCTCTCTGTGAACAAGAAACCAACCAAAGGAGGAGC 6 0.15 No Hit AGGACTTTGTCTGGCCTGTTGATTCTTCGTTTCATGGTCGCCTCATCATT 5 0.125 No Hit CTTCATTTAACATTAAGTCATCTGCTTCTCAAGTGGAGTCTCATAGATTA 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CAACTGTGCTAATGTTTGTTACTGCCAATGGTTTCCACAGAGGTGCAAGC 5 0.125 No Hit TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTCAAAC 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit AGGCATTGGCGCTCGGAGCCTCTGGCATATTCATTGGCCGACCAGTGGTC 5 0.125 No Hit GGGTGCCGTTGAGGGTTACAGAATTGCTGGCGGGCCACTCGGTGAGGTAA 5 0.125 No Hit CTGCATAAATGGTTGGATGATGAGTACTGCCCCGAGGAAACCAATGTTGA 5 0.125 No Hit GGATATTGATACAGGAATGAAGTTAGGAACGAACCACCCAATGGGTCCTT 5 0.125 No Hit ATTATCTTGCCAGTTGCAAAGGTAGCTCTTGACTGTTTATGTTATTAGGG 5 0.125 No Hit AACCCATCTCAAGTTTAGTCCAACTTTTCCAACGGAGGTCAAAAAATTTA 5 0.125 No Hit GGCTCAGCCTCTCCCTCCCCCATGGCTTCTTCTTCTTTGGCTCTGTTATC 5 0.125 No Hit GGAGGAACTAGGCCATCTTTTTTCGCTAGAATTATGTTTATTACATTTTC 5 0.125 No Hit GTTGAGTGAGGATGAGTACGAGTCTCTTGATGATGAAATGGCAGGCCAAG 5 0.125 No Hit AGTGCATCTTCTTTACAAAGAGCATCAGAGTCAACTAGCTGCCTTGCCAA 5 0.125 No Hit GTAATCGAAAAAGTCTTGACATTGACACGAAGGAAGGAAAAATACCTTGT 5 0.125 No Hit GGTTTCATAGACATGAATTTTTTCTACATCAGTTTGGGTGTAGGTTACAT 5 0.125 No Hit AGCCACCTTTTCTCCCTTATATGCAATGGTGGAGCAGCAACTTGGTTGGC 5 0.125 No Hit TTTCGAGAAGGGAAAAGGGTCTCAGAAACTGATTGTCGTGGATTTTACTG 5 0.125 No Hit TGTCGCGCAAGGTGCTAGAATTAGAGGGGCATCTCAAATAATTGGTGTTG 5 0.125 No Hit TCTTTCTCCTTCACAGAGTTTGCTGATTTTTTTGGTCGTTGCAAAATAGT 5 0.125 No Hit TAACATTCCTTACATGTACAGAGTTGGATCAGGAATGACCAAGAACCCCA 5 0.125 No Hit GTTTCTGTGTTGTATACATGTAGCCCAAAGAATAAATTAAAAGGGTTTTG 5 0.125 No Hit CTGTGGGTGTGGCTCTGGCTGCAAGTGCGGCAGCGGCTGTGGAGGATGCA 5 0.125 No Hit AAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAG 5 0.125 No Hit GTGGGATTTTGGGTACATGACTATGCAGAAAGGTTCCAGAGTTTAATGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.5249999999999999 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.8 0.0 0.0 0.0 0.0 94-95 0.9874999999999999 0.0 0.0 0.0 0.0 96-97 1.1375000000000002 0.0 0.0 0.0 0.0 98-99 1.3125 0.0 0.0 0.0 0.0 100-101 1.9125 0.0 0.0 0.0 0.0 102-103 2.3 0.0 0.0 0.0 0.0 104-105 2.475 0.0 0.0 0.0 0.0 106-107 2.7375 0.0 0.0 0.0 0.0 108-109 3.2 0.0 0.0 0.0 0.0 110-111 3.6375 0.0 0.0 0.0 0.0 112-113 4.0625 0.0 0.0 0.0 0.0 114-115 4.5875 0.0 0.0 0.0 0.0 116-117 5.0 0.0 0.0 0.0 0.0 118-119 5.275 0.0 0.0 0.0 0.0 120-121 5.7625 0.0 0.0 0.0 0.0 122-123 6.45 0.0 0.0 0.0 0.0 124-125 6.8375 0.0 0.0 0.0 0.0 126-127 7.55 0.0 0.0 0.0 0.0 128-129 8.2625 0.0 0.0 0.0 0.0 130-131 8.7375 0.0 0.0 0.0 0.0 132-133 9.6 0.0 0.0 0.0 0.0 134-135 10.4125 0.0 0.0 0.0 0.0 136-137 11.3 0.0 0.0 0.0 0.0 138-139 11.787500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGCAT 10 0.006830828 145.0 1 TTGGAGA 25 8.7132835E-4 87.0 6 >>END_MODULE Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256055 spots for SRR13695420.sra Written 1256055 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra Read 1256041 spots for SRR13695420.sra Written 1256041 spots for SRR13695420.sra SRR ids: ['SRR13695420.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_a8h2j0k1 SRR13695420.sra spots: 25120834 blocks: [[1, 1256041], [1256042, 2512082], [2512083, 3768123], [3768124, 5024164], [5024165, 6280205], [6280206, 7536246], [7536247, 8792287], [8792288, 10048328], [10048329, 11304369], [11304370, 12560410], [12560411, 13816451], [13816452, 15072492], [15072493, 16328533], [16328534, 17584574], [17584575, 18840615], [18840616, 20096656], [20096657, 21352697], [21352698, 22608738], [22608739, 23864779], [23864780, 25120834]] SRR13695420 file size 8515458 SRR13695420 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695420 SRR13695420_1.fastq SRR13695420_2.fastq Input file: SRR13695420_1.fastq Paired file: SRR13695420_2.fastq trimmed: SRR13695420-trimmed-pair1.fastq, SRR13695420-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:47:02 2025 >> started Wed Feb 12 01:47:28 2025 >> done (26.362s) 25120834 read pairs processed; of these: 172 ( 0.00%) short read pairs filtered out after trimming by size control 6550 ( 0.03%) empty read pairs filtered out after trimming by size control 25114112 (99.97%) read pairs available; of these: 4014262 (15.98%) trimmed read pairs available after processing 21099850 (84.02%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 3 0.00% 20 0 0.00% 21 2 0.00% 22 2 0.00% 23 3 0.00% 24 0 0.00% 25 4 0.00% 26 6 0.00% 27 2 0.00% 28 4 0.00% 29 2 0.00% 30 0 0.00% 31 4 0.00% 32 15 0.00% 33 5 0.00% 34 11 0.00% 35 2 0.00% 36 3 0.00% 37 13 0.00% 38 21 0.00% 39 13 0.00% 40 34 0.00% 41 23 0.00% 42 31 0.00% 43 41 0.00% 44 29 0.00% 45 37 0.00% 46 38 0.00% 47 71 0.00% 48 80 0.00% 49 83 0.00% 50 135 0.00% 51 137 0.00% 52 140 0.00% 53 155 0.00% 54 197 0.00% 55 220 0.00% 56 214 0.00% 57 289 0.00% 58 338 0.00% 59 429 0.00% 60 462 0.00% 61 590 0.00% 62 674 0.00% 63 793 0.00% 64 837 0.00% 65 947 0.00% 66 1068 0.00% 67 1224 0.00% 68 1432 0.01% 69 1602 0.01% 70 1880 0.01% 71 2020 0.01% 72 2677 0.01% 73 3144 0.01% 74 3537 0.01% 75 3895 0.02% 76 4403 0.02% 77 4631 0.02% 78 5186 0.02% 79 6076 0.02% 80 6739 0.03% 81 7526 0.03% 82 8571 0.03% 83 9586 0.04% 84 10843 0.04% 85 12164 0.05% 86 13073 0.05% 87 14106 0.06% 88 15271 0.06% 89 15933 0.06% 90 17725 0.07% 91 19328 0.08% 92 19688 0.08% 93 22007 0.09% 94 23478 0.09% 95 25758 0.10% 96 27367 0.11% 97 29404 0.12% 98 30130 0.12% 99 31764 0.13% 100 33275 0.13% 101 33671 0.13% 102 36133 0.14% 103 37480 0.15% 104 40316 0.16% 105 41544 0.17% 106 43993 0.18% 107 45310 0.18% 108 46305 0.18% 109 47643 0.19% 110 49321 0.20% 111 50743 0.20% 112 52381 0.21% 113 53398 0.21% 114 56230 0.22% 115 57686 0.23% 116 59621 0.24% 117 62262 0.25% 118 64051 0.26% 119 64499 0.26% 120 66703 0.27% 121 67357 0.27% 122 67745 0.27% 123 69910 0.28% 124 72435 0.29% 125 72738 0.29% 126 75487 0.30% 127 76192 0.30% 128 78853 0.31% 129 78867 0.31% 130 80558 0.32% 131 80517 0.32% 132 81286 0.32% 133 82541 0.33% 134 82567 0.33% 135 84133 0.34% 136 85635 0.34% 137 87682 0.35% 138 88364 0.35% 139 91638 0.36% 140 91298 0.36% 141 93053 0.37% 142 92843 0.37% 143 93076 0.37% 144 95757 0.38% 145 95413 0.38% 146 95631 0.38% 147 97745 0.39% 148 100042 0.40% 149 100155 0.40% 150 101804 0.41% 151 21099850 84.02% 25114112 reads passed initial QC criterion=sequence-density sequence-density=0.45 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=29 prefix-density=0.46 prefix-fanout=1.9 sequence=TGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=49.72 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=8.1 sequence=AAACAGAATATTTACTTTTAGCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATTGAGAACATAGCCAACCT criterion=sequence-density sequence-density=1.20 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=31 prefix-density=1.20 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCATT criterion=fanout-score sequence-density=0.29 sequence-density-rank=10 fanout-score=8.59 fanout-score-rank=1 prefix-density=0.49 prefix-fanout=5.1 sequence=AAGAAAGCTTACCCTAAC SRR13695420 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:48:06 Started mapping on | Feb 12 01:48:06 Finished on | Feb 12 01:50:41 Mapping speed, Million of reads per hour | 583.30 Number of input reads | 25114112 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 23457708 Uniquely mapped reads % | 93.40% Average mapped length | 292.27 Number of splices: Total | 22410374 Number of splices: Annotated (sjdb) | 21932172 Number of splices: GT/AG | 21940435 Number of splices: GC/AG | 375009 Number of splices: AT/AC | 14203 Number of splices: Non-canonical | 80727 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.03% Deletion average length | 3.03 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 587544 % of reads mapped to multiple loci | 2.34% Number of reads mapped to too many loci | 108828 % of reads mapped to too many loci | 0.43% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.69% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1069148 1069148 1069148 N_multimapping 587544 587544 587544 N_noFeature 743413 22855037 1038168 N_ambiguous 468598 2238 159226 UnstrandedReadsAssigned:22245697 PositiveStrandReadsAssigned:600433 NegativeStrandReadsAssigned:22260314 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695420 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695420-trimmed-pair1.fastq SRR13695420-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,114,112 reads, 22,403,436 reads pseudoaligned [quant] estimated average fragment length: 229.829 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,143 rounds 52401 SRR13695420.ke.tsv 34699 SRR13695420.se.tsv 87100 total ==> SRR13695420.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1789.17 774 15.3123 Potri.005G024800.1.v4.1 1035 806.171 486 21.3383 Potri.004G059700.1.v4.1 961 732.241 25 1.20847 Potri.007G009000.2.v4.1 1416 1187.17 0 0 Potri.003G141000.2.v4.1 2943 2714.17 1237.95 16.1442 Potri.016G087400.1.v4.1 270 92.2299 1056 405.268 Potri.015G069301.1.v4.1 564 341.318 0 0 Potri.010G195200.1.v4.1 1773 1544.17 248 5.68469 Potri.012G127500.1.v4.1 977 748.212 448 21.1936 ==> SRR13695420.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 568 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 556 Potri.001G212900.v4.1 222 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 12 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2 SRR13695420 completed mapping pipeline successfully